Standard name
Human Ortholog
Description Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0.09 0 0 0 0 0 0.12 0.08
Bud 0 0 0 0.05 0 0 0 0 0 0 0 0 – – 0 0 0.1 0.05 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0.1 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0.08 0.05 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0.06 0.12 0.07 0.05 0.05 0 0.07 0.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0.08 0 0.09 0.05 0.12 0.05 0.13 0.19 0.13
Mitochondria 0.9 0.93 0.96 0.95 0.92 0.93 0.91 0.87 0.91 0.91 0.94 0.93 – – 0.71 0.05 0.07 0 0.62 0.65 0.69 0.55 0.4 0.45
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.08 0.07 0.08 0.06 0.08 0.12 0.11 0.07 0.08 0.09 0.06 0.06 – – 0.06 0.08 0.06 0.09 0 0 0 0 0 0
Nucleolus 0.09 0 0 0 0.07 0.09 0.14 0.13 0.12 0.1 0.11 0.09 – – 0.18 0.1 0.09 0.13 0 0 0 0 0 0
Peroxisomes 0.09 0.06 0.08 0.14 0 0 0 0 0 0.05 0 0 – – 0.06 0.51 0.47 0.35 0.18 0.11 0.17 0.15 0.18 0.13
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0.12 0 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0 0.08 0 0 0 0 0 0 0 0 0 0 – – 0.06 0 0.05 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 2 1 1 4 1 1 1 0 0 6 – – 0 4 8 1 4 13 2 0 4 3
Bud 5 4 8 14 13 10 21 21 21 15 18 28 – – 0 4 9 4 0 0 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 – – 0 0 0 0 1 0 0 0 0 0
Bud Site 4 0 0 2 1 5 7 7 6 7 4 9 – – 0 1 4 2 – – – – – –
Cell Periphery 5 9 0 2 0 0 2 0 2 1 5 0 – – 0 3 9 2 0 0 0 0 0 0
Cytoplasm 1 2 2 7 9 8 8 4 5 5 3 5 – – 0 10 5 7 0 0 0 0 0 0
Endoplasmic Reticulum 1 2 2 0 1 3 0 2 1 1 5 0 – – 0 2 1 0 0 0 0 0 0 0
Endosome 9 2 1 3 9 1 3 3 1 4 0 1 – – 0 4 6 10 20 15 8 0 2 4
Golgi 0 9 1 1 0 0 0 0 0 1 2 2 – – 0 10 2 8 13 39 9 2 7 5
Mitochondria 239 301 173 275 399 411 687 506 513 688 598 883 – – 12 6 7 1 173 216 123 11 16 20
Nucleus 4 0 0 0 1 2 4 4 1 0 2 0 – – 0 2 2 1 0 0 0 0 0 0
Nuclear Periphery 20 23 14 17 34 53 82 39 46 69 37 58 – – 1 10 6 8 2 3 1 0 0 0
Nucleolus 25 5 5 8 30 40 102 77 69 72 67 88 – – 3 12 8 11 1 0 0 0 0 1
Peroxisomes 23 21 15 41 16 19 23 26 19 38 12 40 – – 1 63 44 30 51 37 30 3 7 5
SpindlePole 5 0 3 7 1 1 6 12 9 10 7 7 – – 0 15 3 4 5 0 0 0 0 0
Vac/Vac Membrane 5 26 5 5 8 7 8 15 6 5 3 8 – – 1 4 5 5 1 3 0 0 0 0
Unique Cell Count 266 324 180 291 434 442 751 579 563 754 635 945 17 124 94 85 281 334 179 21 42 45
Labelled Cell Count 346 405 231 383 523 564 954 718 700 916 763 1135 18 150 119 94 281 334 179 21 42 45


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.7 9.0 6.9 7.7 8.0 8.6 8.9 9.7 11.7 13.2 14.8 15.1 – – 8.5 11.0 11.2 11.9 – 9.5 –
Std Deviation (1e-4) 1.7 2.0 1.5 1.8 2.0 2.3 2.5 3.3 4.2 5.0 6.4 6.8 – – 1.2 3.5 4.1 3.3 – 1.8 –
Intensity Change (Log2) – – – 0.15 0.21 0.31 0.37 0.49 0.76 0.93 1.1 1.13 – – 0.29 0.67 0.7 0.78 – 0.46 –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Bud 0.2 -0.9 -1.5 -1.1 -0.5 -0.4 0 -1.1 -1.0 – – 0 0 1.7 0
Bud Neck 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Mitochondria -0.8 -1.9 -1.5 -2.1 -3.3 -2.2 -2.2 -1.0 -1.4 – – -4.2 -15.9 -14.7 -15.2
Nucleus 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Nuclear Periphery -0.8 0 1.5 1.2 -0.5 0.2 0.6 -1.0 -0.8 – – 0 0.1 -0.4 0.4
Nucleolus 0 2.0 2.7 4.1 4.0 3.7 3.0 3.2 2.9 – – 0 2.6 0 3.2
Peroxisomes 1.9 -2.4 -2.0 -3.2 -2.0 -2.8 -1.7 -4.3 -2.3 – – 0 8.3 7.4 5.5
SpindlePole 0 0 0 0 0 0 0 0 0 – – 0 3.8 0 0
Vacuole 0 0 0 0 0 0 0 0 0 – – 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 27.0456 30.8067 28.8496 24.8335 26.3056 27.2723 20.0653 25.6489 23.8418 22.8825 21.0388 24.4355 23.9834 27.7783 26.5555 21.6162 23.9548 24.732
Actin 0.0092 0.0154 0.0107 0.0063 0.0019 0.0036 0.028 0.0038 0.0199 0.0041 0.0074 0.0015 0.0068 0.0075 0.0059 0.001 0.0013 0.0006
Bud 0 0 0 0 0 0 0.0003 0 0.0001 0 0.0001 0 0 0.0001 0 0 0 0
Bud Neck 0.0001 0.0001 0 0 0 0 0.0022 0.0001 0.0046 0.0001 0 0 0.0002 0.0001 0 0 0 0
Bud Periphery 0 0.0001 0.0001 0 0 0.0001 0.0005 0 0.0002 0 0.0003 0 0 0.0001 0.0001 0 0 0
Bud Site 0.0002 0.0001 0.0001 0 0 0 0.0032 0.0002 0.0014 0.0001 0.0002 0 0.0001 0.0002 0.0001 0 0.0001 0
Cell Periphery 0.0001 0.0003 0.0001 0 0.0001 0.0001 0.0003 0.0001 0.0001 0.0002 0.0001 0 0.0001 0.0002 0.0001 0 0 0.0001
Cytoplasm 0 0 0 0 0 0 0.0013 0 0.0001 0 0 0 0.0002 0 0 0 0 0
Cytoplasmic Foci 0.0014 0.0011 0.0005 0.0003 0.0005 0.0003 0.017 0.0016 0.0027 0.0001 0.0011 0.001 0.0023 0.0011 0.0008 0.0022 0.003 0.0003
Eisosomes 0.0007 0.0015 0.0033 0.0004 0.0003 0.0008 0.0007 0.0004 0.0009 0.0044 0.0004 0.0003 0.0004 0.0003 0.0012 0.0002 0.0003 0.0002
Endoplasmic Reticulum 0 0 0 0 0 0.0002 0.0025 0 0.0002 0 0.0002 0 0.0002 0 0 0 0 0
Endosome 0.0009 0.0016 0.0011 0.001 0.0004 0.0018 0.0153 0.0005 0.0036 0.0006 0.0047 0.0002 0.002 0.0018 0.0008 0.0009 0.0012 0.0008
Golgi 0.008 0.0203 0.0138 0.0196 0.0034 0.0205 0.0162 0.0103 0.0093 0.0032 0.0056 0.0023 0.0062 0.0208 0.0104 0.0103 0.0044 0.0098
Lipid Particles 0.023 0.0296 0.017 0.007 0.0396 0.0202 0.0526 0.0218 0.0146 0.007 0.0361 0.0179 0.0398 0.0319 0.0399 0.0303 0.0542 0.022
Mitochondria 0.9344 0.9026 0.9354 0.9607 0.9323 0.9317 0.8013 0.9322 0.9117 0.9697 0.872 0.9537 0.9137 0.9047 0.922 0.9265 0.9071 0.9489
None 0.0002 0 0 0 0 0.0001 0.0037 0 0.0003 0 0.0003 0 0.0006 0 0 0 0 0
Nuclear Periphery 0.0001 0.0001 0 0 0 0.0036 0.0054 0 0.0004 0 0.0023 0 0.0009 0 0 0 0 0
Nucleolus 0.0034 0.0004 0.0001 0.0001 0.0001 0.0005 0.0009 0.0002 0.005 0.0001 0.0004 0 0.0004 0.0003 0.0002 0 0.0003 0.0001
Nucleus 0.0003 0.0001 0 0 0 0.0006 0.001 0 0.0003 0 0.0003 0 0.0003 0 0 0 0 0
Peroxisomes 0.0105 0.0098 0.011 0.0024 0.0123 0.0025 0.0366 0.0261 0.0134 0.0077 0.0204 0.0223 0.0129 0.0112 0.0076 0.0271 0.0251 0.0039
Punctate Nuclear 0.0002 0 0 0 0 0.0007 0.0025 0 0.0003 0 0.0007 0 0.0026 0 0 0 0 0
Vacuole 0.0005 0.0011 0.0003 0.0002 0.0003 0.0013 0.0014 0.0003 0.0012 0.0002 0.0016 0.0001 0.0004 0.0015 0.0006 0.0001 0.0004 0.0005
Vacuole Periphery 0.0067 0.0157 0.0063 0.002 0.0086 0.0113 0.0072 0.0022 0.0096 0.0024 0.0458 0.0007 0.0098 0.0181 0.0102 0.0013 0.0023 0.0127

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 102.9844 69.516 73.8904 121.8841 83.1118 75.6201 89.7592 87.827 86.3358 93.1895
Translational Efficiency 2.0715 2.5233 2.6405 1.4559 2.5496 2.8795 1.8298 1.9139 2.1396 2.1825

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1830 1518 2139 610 1125 1443 1869 1545 2955 2961 4008 2155

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 852.01 1014.71 1272.19 1455.46 896.16 1125.10 1289.69 1620.22 868.82 1068.51 1280.35 1573.58
Standard Deviation 169.18 249.57 231.97 307.29 169.51 260.13 220.36 389.64 170.66 260.67 226.80 375.61
Intensity Change Log 2 — 0.252125 0.578372 0.772533 — 0.328225 0.525196 0.854361 — 0.291637 0.551357 0.815060

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.005121 0.025594 0.019081 0.017776 0.020807 0.021667 0.011281 0.012438 0.011093 0.023680 0.015443 0.013949
Bud Neck 0.002412 0.002911 0.001207 0.003022 0.002588 0.001777 0.001457 0.001345 0.002479 0.002359 0.001323 0.001820
Bud Site 0.003591 0.004048 0.001173 0.003758 0.003319 0.001970 0.001936 0.002049 0.003488 0.003035 0.001529 0.002533
Cell Periphery 0.000373 0.000463 0.000091 0.000310 0.000308 0.000412 0.000088 0.000389 0.000348 0.000438 0.000089 0.000367
Cytoplasm 0.001161 0.001037 0.000465 0.000600 0.001662 0.000823 0.001162 0.000620 0.001352 0.000933 0.000790 0.000614
Cytoplasmic Foci 0.018990 0.021498 0.026557 0.020491 0.040962 0.020615 0.019664 0.005104 0.027355 0.021068 0.023343 0.009459
Eisosomes 0.000227 0.001236 0.000663 0.000648 0.000716 0.001466 0.000790 0.001291 0.000413 0.001348 0.000722 0.001109
Endoplasmic Reticulum 0.000322 0.000292 0.000275 0.001244 0.000398 0.000236 0.000110 0.000222 0.000351 0.000264 0.000198 0.000511
Endosome 0.016752 0.013985 0.009826 0.011630 0.025343 0.007354 0.007626 0.002923 0.020023 0.010754 0.008800 0.005388
Golgi 0.036635 0.059390 0.085844 0.076437 0.101674 0.053963 0.042756 0.023974 0.061396 0.056745 0.065752 0.038824
Lipid Particles 0.027508 0.018406 0.019763 0.007064 0.048922 0.016890 0.010959 0.001045 0.035661 0.017668 0.015658 0.002749
Mitochondria 0.840889* 0.775946* 0.803089* 0.809022* 0.641031* 0.822832* 0.862430* 0.928181* 0.764801* 0.798795* 0.830761* 0.894452*
Mitotic Spindle 0.002770 0.001204 0.003340 0.006366 0.001503 0.000862 0.003845 0.000909 0.002288 0.001038 0.003575 0.002454
None 0.001271 0.000836 0.001168 0.001326 0.000728 0.000911 0.000903 0.000977 0.001064 0.000873 0.001044 0.001076
Nuclear Periphery 0.000263 0.000108 0.000861 0.001225 0.000204 0.000158 0.000523 0.000107 0.000241 0.000132 0.000704 0.000423
Nuclear Periphery Foci 0.000527 0.000297 0.000441 0.000545 0.000545 0.000137 0.000327 0.000083 0.000534 0.000219 0.000388 0.000213
Nucleolus 0.000365 0.000812 0.000093 0.000349 0.000228 0.000469 0.000160 0.000301 0.000313 0.000645 0.000124 0.000315
Nucleus 0.000295 0.000521 0.000034 0.000061 0.000164 0.000504 0.000042 0.000049 0.000245 0.000513 0.000038 0.000053
Peroxisomes 0.037247 0.066572 0.015184 0.020390 0.106097* 0.042251 0.029226 0.007637 0.063459 0.054720 0.021732 0.011247
Vacuole 0.001675 0.002679 0.003363 0.007723 0.001342 0.002230 0.002206 0.005142 0.001548 0.002460 0.002824 0.005872
Vacuole Periphery 0.001604 0.002163 0.007482 0.010012 0.001460 0.002471 0.002511 0.005214 0.001549 0.002314 0.005164 0.006572

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.51 -8.08 -4.43 2.14 0.39 -0.32 4.28 3.40 3.67 -0.59 -7.25 -3.32 -1.78 4.72 0.87
Bud Neck -0.58 2.13 -0.80 -0.18 -3.34 1.14 1.80 1.76 0.85 0.26 0.23 2.72 1.28 1.14 -1.57
Bud Site -0.33 2.69 -0.12 0.22 -2.92 1.44 1.64 1.33 -0.11 -0.19 0.60 3.05 1.26 0.67 -1.85
Cell Periphery -0.50 3.59 0.60 0.89 -2.75 -0.42 4.06 -0.60 0.08 -2.38 -0.57 4.87 -0.15 0.42 -2.98
Cytoplasm 0.31 1.70 1.35 2.54 -1.76 1.94 0.95 2.20 0.62 1.24 1.33 1.67 2.10 1.42 0.69
Cytoplasmic Foci -1.51 -4.54 -0.61 0.40 2.44 8.38 9.03 16.85 11.29 11.60 4.58 2.97 13.90 10.22 12.43
Eisosomes -6.96 -10.10 -3.40 3.11 0.11 -5.09 -0.88 -2.79 0.73 -2.45 -9.17 -7.18 -4.71 1.36 -2.60
Endoplasmic Reticulum 0.48 0.98 -1.48 -1.53 -1.56 2.45 5.31 2.80 0.26 -3.20 1.86 4.17 -0.88 -1.37 -1.76
Endosome 1.52 4.51 2.51 1.18 -1.09 8.34 8.40 10.92 4.45 5.32 6.85 9.06 11.58 5.62 4.37
Golgi -5.79 -11.68 -5.84 -2.36 1.28 8.35 10.83 14.69 7.98 5.64 1.43 -1.33 6.63 5.41 8.07
Lipid Particles 3.32 2.89 7.86 5.21 6.06 7.70 9.78 12.82 8.56 9.13 7.73 9.10 16.33 11.40 12.20
Mitochondria 6.45 4.23 2.43 -2.38 -0.44 -14.09 -18.26 -24.64 -12.51 -9.16 -4.29 -9.06 -17.11 -13.51 -10.06
Mitotic Spindle 2.49 -0.68 -2.30 -3.52 -1.93 1.86 -3.43 1.83 -0.06 4.28 3.03 -2.24 -0.29 -2.96 1.79
None 0.82 0.21 -0.09 -3.56 -1.48 -3.26 -3.24 -3.71 -0.96 -1.10 0.59 0.08 -0.02 -2.79 -0.55
Nuclear Periphery 2.64 -4.48 -1.87 -2.18 -0.70 1.02 -2.57 2.20 2.12 3.51 2.67 -4.97 -1.21 -2.00 1.66
Nuclear Periphery Foci 1.79 0.63 -0.08 -1.60 -0.64 2.92 1.37 3.33 2.51 3.07 3.34 1.39 3.23 0.11 2.43
Nucleolus -0.74 1.60 0.40 0.99 -2.00 -0.88 1.29 -0.64 0.57 -1.30 -0.94 1.73 0.31 1.18 -2.21
Nucleus -0.46 1.96 1.79 1.49 -2.50 -0.80 1.90 1.74 1.08 -0.67 -0.81 2.34 2.18 1.77 -1.65
Peroxisomes -6.14 7.43 3.90 8.76 -1.40 9.77 12.45 16.91 10.43 8.54 2.31 13.55 17.14 15.25 5.81
Vacuole -1.82 -3.56 -4.46 -3.57 -3.14 -1.81 -3.06 -3.66 -2.57 -2.79 -2.42 -4.37 -5.12 -3.82 -3.54
Vacuole Periphery -1.79 -10.45 -7.30 -6.64 -2.00 -1.80 -3.96 -3.97 -2.50 -2.80 -2.40 -11.05 -6.67 -5.25 -1.75
Standard name
Human Ortholog
Description Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis
Localization
Cell Percentages mitochondrion (86%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Atp7

Atp7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atp7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available