Standard name | |
---|---|
Human Ortholog | |
Description | Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 7 | 10 | 29 | 4 | 14 | 3 | 13 | – | 6 | 5 | 0 | 7 | 8 | 1 | 2 | 17 | 5 | 2 | 9 | 22 | 11 | 13 | 20 | 33 |
Bud | 12 | 15 | 20 | 15 | 27 | 24 | 32 | – | 60 | 37 | 17 | 29 | 23 | 16 | 13 | 11 | 5 | 7 | 1 | 4 | 0 | 0 | 1 | 3 |
Bud Neck | 0 | 0 | 5 | 0 | 1 | 1 | 3 | – | 1 | 1 | 3 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 1 | 1 | 3 | 4 | – | 15 | 16 | 15 | 13 | 1 | 0 | 0 | 2 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 1 | 1 | 0 | 1 | 2 | – | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 7 | 15 | 11 | 21 | 14 | 26 | – | 8 | 13 | 12 | 8 | 13 | 16 | 14 | 32 | 16 | 32 | 0 | 0 | 0 | 0 | 1 | 0 |
Endoplasmic Reticulum | 7 | 15 | 6 | 2 | 5 | 4 | 6 | – | 4 | 2 | 2 | 2 | 9 | 5 | 3 | 10 | 6 | 10 | 1 | 0 | 1 | 4 | 3 | 4 |
Endosome | 22 | 31 | 34 | 25 | 37 | 26 | 23 | – | 7 | 1 | 0 | 7 | 19 | 62 | 83 | 51 | 57 | 52 | 63 | 85 | 98 | 53 | 62 | 64 |
Golgi | 19 | 21 | 11 | 8 | 2 | 0 | 0 | – | 0 | 0 | 0 | 1 | 11 | 18 | 8 | 16 | 13 | 5 | 86 | 115 | 49 | 68 | 85 | 85 |
Mitochondria | 172 | 267 | 89 | 120 | 115 | 245 | 263 | – | 375 | 392 | 259 | 216 | 234 | 157 | 208 | 33 | 26 | 25 | 21 | 74 | 69 | 53 | 56 | 35 |
Nucleus | 1 | 1 | 2 | 2 | 3 | 4 | 4 | – | 2 | 1 | 4 | 6 | 0 | 0 | 1 | 6 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 1 | 2 | 0 | 1 | 1 | 2 | 2 | – | 2 | 5 | 0 | 0 | 2 | 2 | 4 | 3 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 |
Nucleolus | 1 | 1 | 0 | 0 | 1 | 3 | 11 | – | 9 | 17 | 8 | 4 | 4 | 4 | 8 | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 |
Peroxisomes | 8 | 22 | 24 | 29 | 25 | 40 | 29 | – | 21 | 22 | 8 | 15 | 32 | 57 | 74 | 6 | 7 | 7 | 41 | 39 | 71 | 49 | 70 | 71 |
SpindlePole | 2 | 8 | 59 | 29 | 69 | 61 | 76 | – | 43 | 64 | 35 | 58 | 6 | 20 | 16 | 67 | 53 | 50 | 1 | 7 | 7 | 5 | 3 | 6 |
Vac/Vac Membrane | 8 | 21 | 11 | 2 | 6 | 6 | 6 | – | 5 | 5 | 1 | 5 | 5 | 5 | 7 | 13 | 14 | 12 | 1 | 12 | 4 | 2 | 2 | 3 |
Unique Cell Count | 206 | 321 | 219 | 193 | 256 | 335 | 382 | 425 | 452 | 289 | 266 | 295 | 288 | 343 | 208 | 156 | 166 | 230 | 369 | 322 | 258 | 313 | 313 | |
Labelled Cell Count | 263 | 423 | 306 | 250 | 328 | 437 | 500 | 558 | 581 | 364 | 371 | 369 | 365 | 443 | 270 | 208 | 206 | 230 | 369 | 322 | 258 | 313 | 313 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.0 | 6.1 | 6.0 | 5.5 | 5.9 | 4.7 | 4.9 | – | 4.2 | 4.0 | 3.7 | 4.4 | 5.3 | 5.6 | 5.6 | 6.7 | 7.2 | 6.3 | 5.2 | 5.3 | 5.8 |
Std Deviation (1e-4) | 0.7 | 0.9 | 1.1 | 1.3 | 1.3 | 1.0 | 1.4 | – | 0.7 | 0.8 | 0.7 | 0.9 | 0.8 | 1.1 | 1.2 | 1.2 | 1.6 | 1.2 | 1.1 | 1.0 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.04 | -0.36 | -0.3 | – | -0.54 | -0.61 | -0.72 | -0.45 | -0.18 | -0.1 | -0.12 | 0.15 | 0.26 | 0.06 | -0.23 | -0.19 | -0.07 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -4.2 | -2.9 | 0 | -4.6 | – | 0 | 0 | 0 | 0 | -4.6 | -6.1 | -6.4 | -1.7 | -3.3 | -4.3 |
Bud | -0.5 | 0.5 | 0 | -0.3 | – | 0 | 0 | 0 | 0 | -0.5 | -1.6 | -2.6 | -1.5 | -2.3 | -1.9 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.5 | 0.6 | -1.4 | 0 | – | -3.2 | -2.4 | -1.3 | -2.0 | -1.2 | -0.6 | -1.4 | 2.8 | 1.2 | 3.7 |
Endoplasmic Reticulum | 0 | -0.6 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0.2 | 0 | 0 | 1.1 | 0 | 1.6 |
Endosome | -0.7 | -0.3 | 0 | -3.8 | – | 0 | 0 | 0 | 0 | -3.3 | 1.7 | 2.5 | 2.3 | 4.7 | 3.7 |
Golgi | -0.4 | -2.8 | 0 | 0 | – | 0 | 0 | 0 | 0 | -0.7 | 0.6 | -1.7 | 1.1 | 1.3 | -1.0 |
Mitochondria | 4.4 | 0.9 | 0 | 6.8 | – | 0 | 0 | 0 | 0 | 9.0 | 3.1 | 4.6 | -5.7 | -5.0 | -5.4 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1.2 | -0.4 | 0 | -1.4 | – | 0 | 0 | 0 | 0 | 0 | 2.7 | 3.2 | -3.3 | -2.2 | -2.4 |
SpindlePole | -2.9 | 0 | 0 | -2.0 | – | 0 | 0 | 0 | 0 | -8.4 | -6.1 | -7.6 | 1.2 | 1.5 | 0.7 |
Vacuole | -2.3 | -1.6 | -2.2 | -2.5 | – | -3.0 | -3.1 | -3.4 | -1.9 | -2.1 | -2.1 | -2.0 | 0.6 | 1.5 | 0.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.0743 | 0.5272 | 0.8401 | 0.3711 | 0.7544 | 0.7919 | 1.1756 | 1.7658 | 1.7003 | 1.7919 | 1.5725 | 1.5351 | 0.5909 | 1.1357 | 0.837 | 1.5795 | 0.0573 | 0.7366 |
Actin | 0.0753 | 0.0112 | 0.0318 | 0.0173 | 0.0243 | 0.0165 | 0.043 | 0.0253 | 0.0225 | 0.0337 | 0.0703 | 0.0216 | 0.0252 | 0.0237 | 0.0566 | 0.0717 | 0.0065 | 0.0222 |
Bud | 0.0011 | 0.0019 | 0.0007 | 0.0004 | 0.0005 | 0.0003 | 0.0003 | 0.0008 | 0.0062 | 0.0004 | 0 | 0.0003 | 0.0007 | 0.0029 | 0.0006 | 0.0075 | 0.0002 | 0.0001 |
Bud Neck | 0.0078 | 0.0008 | 0.0027 | 0.0019 | 0.0004 | 0.0015 | 0.0008 | 0.0006 | 0.0012 | 0.001 | 0.0002 | 0.0011 | 0.0014 | 0.0007 | 0.001 | 0.0025 | 0.0014 | 0.0017 |
Bud Periphery | 0.0014 | 0.0014 | 0.0009 | 0.0005 | 0.0008 | 0.0003 | 0.0003 | 0.0006 | 0.0051 | 0.0005 | 0 | 0.0003 | 0.0012 | 0.003 | 0.0006 | 0.0148 | 0.0002 | 0.0002 |
Bud Site | 0.0201 | 0.0133 | 0.0022 | 0.0029 | 0.0006 | 0.0007 | 0.0011 | 0.0084 | 0.0096 | 0.0009 | 0.0001 | 0.0009 | 0.0034 | 0.0048 | 0.0014 | 0.0417 | 0.0005 | 0.0004 |
Cell Periphery | 0.0005 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0.0046 | 0.0001 | 0.0001 |
Cytoplasm | 0.0091 | 0.0181 | 0.015 | 0.002 | 0.0019 | 0.0021 | 0.0033 | 0.0094 | 0.0175 | 0.0019 | 0.0007 | 0.0041 | 0.0018 | 0.0161 | 0.0048 | 0.0032 | 0.0012 | 0.0016 |
Cytoplasmic Foci | 0.1247 | 0.2222 | 0.1842 | 0.1397 | 0.1651 | 0.1652 | 0.1695 | 0.2076 | 0.2012 | 0.1471 | 0.1602 | 0.139 | 0.1971 | 0.2004 | 0.1268 | 0.1446 | 0.1429 | 0.1393 |
Eisosomes | 0.0007 | 0.0001 | 0.0004 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0054 | 0.0009 | 0.0014 | 0.0019 | 0.003 | 0.0007 | 0.0013 | 0.0008 | 0.0003 | 0.0003 | 0.0003 | 0.0004 | 0.001 | 0.0022 | 0.0005 | 0.0022 | 0.0005 | 0.0004 |
Endosome | 0.1968 | 0.2315 | 0.2641 | 0.3348 | 0.2194 | 0.1984 | 0.2218 | 0.2257 | 0.1778 | 0.1908 | 0.1841 | 0.1477 | 0.1954 | 0.2333 | 0.1264 | 0.1438 | 0.2237 | 0.1765 |
Golgi | 0.1737 | 0.2269 | 0.2316 | 0.2134 | 0.2413 | 0.3341 | 0.2128 | 0.2643 | 0.2628 | 0.2481 | 0.2817 | 0.289 | 0.1711 | 0.2565 | 0.3016 | 0.2275 | 0.32 | 0.3446 |
Lipid Particles | 0.1409 | 0.0783 | 0.0693 | 0.0998 | 0.1035 | 0.0497 | 0.0651 | 0.0181 | 0.0288 | 0.0208 | 0.111 | 0.0847 | 0.153 | 0.0643 | 0.0784 | 0.0762 | 0.1772 | 0.092 |
Mitochondria | 0.0486 | 0.037 | 0.0361 | 0.0552 | 0.0927 | 0.0428 | 0.0201 | 0.0223 | 0.0361 | 0.0179 | 0.0029 | 0.0569 | 0.0188 | 0.0305 | 0.0444 | 0.1145 | 0.0222 | 0.0326 |
None | 0.0051 | 0.0202 | 0.0051 | 0.0023 | 0.0012 | 0.0006 | 0.0007 | 0.0068 | 0.0007 | 0.0002 | 0.0001 | 0.0002 | 0.0004 | 0.013 | 0.0073 | 0.0017 | 0.0002 | 0.0002 |
Nuclear Periphery | 0.0248 | 0.0006 | 0.0009 | 0.0025 | 0.0194 | 0.0038 | 0.0024 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0004 | 0.0017 | 0.0016 | 0.0028 | 0.0002 | 0.0001 |
Nucleolus | 0.0015 | 0.0003 | 0.0003 | 0.0006 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0006 | 0.0002 | 0.0016 | 0.0001 | 0.0001 |
Nucleus | 0.0227 | 0.0004 | 0.0003 | 0.0007 | 0.0015 | 0.0005 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0004 | 0.0006 | 0.0006 | 0.0025 | 0.0001 | 0 |
Peroxisomes | 0.1221 | 0.1271 | 0.1466 | 0.1122 | 0.1168 | 0.1754 | 0.2514 | 0.205 | 0.2216 | 0.3336 | 0.1867 | 0.2499 | 0.2161 | 0.1407 | 0.2401 | 0.1137 | 0.0984 | 0.185 |
Punctate Nuclear | 0.0092 | 0.0014 | 0.0012 | 0.0029 | 0.0015 | 0.0042 | 0.001 | 0.001 | 0.0043 | 0.0005 | 0.0002 | 0.0005 | 0.0011 | 0.001 | 0.0052 | 0.0016 | 0.0004 | 0.0005 |
Vacuole | 0.0048 | 0.0046 | 0.0029 | 0.0045 | 0.0018 | 0.0013 | 0.003 | 0.0014 | 0.0022 | 0.0011 | 0.0006 | 0.0017 | 0.0085 | 0.0024 | 0.0008 | 0.0158 | 0.0022 | 0.0011 |
Vacuole Periphery | 0.0037 | 0.0015 | 0.0022 | 0.0038 | 0.0038 | 0.0014 | 0.0019 | 0.001 | 0.0012 | 0.0008 | 0.0009 | 0.0013 | 0.0021 | 0.0014 | 0.0008 | 0.0046 | 0.0017 | 0.0014 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.4444 | 10.7308 | 12.7404 | 13.5669 | 6.848 | 9.118 | 13.7794 | 15.0853 | 11.544 | 14.0657 |
Translational Efficiency | 0.4679 | 0.5026 | 0.4325 | 0.4958 | 0.7767 | 0.6458 | 0.5867 | 0.4545 | 0.561 | 0.5013 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2056 | 89 | 385 | 1484 | 1071 | 1933 | 2296 | 2678 | 3127 | 2022 | 2681 | 4162 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 722.65 | 901.49 | 1021.64 | 911.72 | 675.22 | 811.06 | 933.20 | 960.84 | 706.41 | 815.04 | 945.90 | 943.33 |
Standard Deviation | 78.52 | 124.91 | 108.66 | 109.15 | 94.41 | 106.70 | 103.11 | 116.58 | 87.25 | 109.16 | 108.46 | 116.39 |
Intensity Change Log 2 | — | 0.319014 | 0.499518 | 0.335294 | — | 0.264451 | 0.466829 | 0.508939 | — | 0.292916 | 0.483820 | 0.421782 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002760 | 0.015757 | 0.033913 | 0.034148 | 0.001906 | 0.002689 | 0.010578 | 0.012637 | 0.002468 | 0.003264 | 0.013929 | 0.020307 |
Bud Neck | 0.002397 | 0.000727 | 0.003526 | 0.004477 | 0.002033 | 0.000625 | 0.001983 | 0.003612 | 0.002272 | 0.000630 | 0.002205 | 0.003920 |
Bud Site | 0.002904 | 0.013187 | 0.022115 | 0.037860 | 0.003379 | 0.002010 | 0.009715 | 0.022732 | 0.003066 | 0.002502 | 0.011496 | 0.028126 |
Cell Periphery | 0.000076 | 0.000137 | 0.000115 | 0.000077 | 0.000125 | 0.000053 | 0.000038 | 0.000047 | 0.000093 | 0.000057 | 0.000049 | 0.000058 |
Cytoplasm | 0.004848 | 0.003122 | 0.006451 | 0.031618 | 0.005013 | 0.002634 | 0.008703 | 0.025633 | 0.004904 | 0.002655 | 0.008379 | 0.027767 |
Cytoplasmic Foci | 0.201798* | 0.280224* | 0.310951* | 0.325777* | 0.195490* | 0.201859* | 0.360262* | 0.297539* | 0.199637* | 0.205308* | 0.353180* | 0.307608* |
Eisosomes | 0.000173 | 0.000103 | 0.000099 | 0.000102 | 0.000143 | 0.000087 | 0.000062 | 0.000058 | 0.000163 | 0.000088 | 0.000067 | 0.000074 |
Endoplasmic Reticulum | 0.000162 | 0.000279 | 0.003650 | 0.000804 | 0.000183 | 0.000132 | 0.000667 | 0.000286 | 0.000169 | 0.000138 | 0.001095 | 0.000471 |
Endosome | 0.118865 | 0.171930 | 0.142270 | 0.147901 | 0.151817 | 0.183067 | 0.197943 | 0.172846 | 0.130151 | 0.182577 | 0.189948 | 0.163952 |
Golgi | 0.489914* | 0.374939* | 0.097932 | 0.130087* | 0.450442* | 0.497778* | 0.175299* | 0.177196* | 0.476395* | 0.492371* | 0.164189* | 0.160399* |
Lipid Particles | 0.022597 | 0.007509 | 0.026414 | 0.006226 | 0.032914 | 0.013292 | 0.012044 | 0.003523 | 0.026130 | 0.013037 | 0.014107 | 0.004487 |
Mitochondria | 0.078826 | 0.030446 | 0.003881 | 0.006838 | 0.085265 | 0.051453 | 0.014785 | 0.027698 | 0.081032 | 0.050528 | 0.013219 | 0.020260 |
Mitotic Spindle | 0.004592 | 0.021852 | 0.217125* | 0.079018 | 0.006275 | 0.005205 | 0.112305* | 0.131566* | 0.005168 | 0.005938 | 0.127357* | 0.112830* |
None | 0.000121 | 0.000019 | 0.000052 | 0.000043 | 0.000232 | 0.000067 | 0.000066 | 0.000079 | 0.000159 | 0.000065 | 0.000064 | 0.000066 |
Nuclear Periphery | 0.000027 | 0.000019 | 0.000348 | 0.000227 | 0.000053 | 0.000054 | 0.000185 | 0.000184 | 0.000036 | 0.000053 | 0.000208 | 0.000199 |
Nuclear Periphery Foci | 0.000144 | 0.000115 | 0.005898 | 0.000935 | 0.000221 | 0.000195 | 0.001295 | 0.000394 | 0.000171 | 0.000191 | 0.001956 | 0.000587 |
Nucleolus | 0.000192 | 0.000046 | 0.000238 | 0.000132 | 0.000463 | 0.000071 | 0.000385 | 0.000194 | 0.000285 | 0.000070 | 0.000364 | 0.000172 |
Nucleus | 0.000120 | 0.000064 | 0.002639 | 0.001484 | 0.000610 | 0.000148 | 0.000640 | 0.001139 | 0.000288 | 0.000145 | 0.000927 | 0.001262 |
Peroxisomes | 0.065493 | 0.073280 | 0.095014 | 0.145291* | 0.058503 | 0.032352 | 0.068330 | 0.077735 | 0.063099 | 0.034153 | 0.072162 | 0.101823* |
Vacuole | 0.002104 | 0.003204 | 0.025969 | 0.044939 | 0.002043 | 0.003474 | 0.022689 | 0.040859 | 0.002083 | 0.003462 | 0.023160 | 0.042314 |
Vacuole Periphery | 0.001888 | 0.003044 | 0.001401 | 0.002016 | 0.002893 | 0.002754 | 0.002027 | 0.004042 | 0.002232 | 0.002767 | 0.001937 | 0.003320 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.29 | -6.30 | -10.94 | -1.61 | 0.10 | -1.42 | -7.82 | -9.10 | -8.22 | -1.17 | -1.18 | -9.56 | -13.79 | -12.13 | -3.50 |
Bud Neck | 4.14 | -2.39 | -5.38 | -10.20 | -1.37 | 3.92 | -0.49 | -5.60 | -11.38 | -6.44 | 5.80 | -0.74 | -7.18 | -15.09 | -7.50 |
Bud Site | -1.38 | -5.87 | -14.84 | -3.44 | -4.01 | 1.43 | -5.96 | -13.06 | -16.21 | -9.34 | 0.90 | -10.06 | -20.63 | -20.81 | -12.21 |
Cell Periphery | -1.05 | -3.75 | -0.48 | 0.98 | 4.00 | 4.95 | 6.40 | 5.70 | 0.92 | -3.75 | 3.84 | 5.48 | 4.34 | -0.45 | -2.81 |
Cytoplasm | 1.35 | -1.43 | -12.70 | -12.50 | -9.46 | 2.68 | -4.69 | -15.07 | -17.45 | -11.67 | 3.71 | -5.63 | -20.10 | -21.79 | -15.02 |
Cytoplasmic Foci | -4.85 | -8.50 | -18.63 | -1.95 | -0.99 | -1.14 | -26.96 | -16.06 | -17.83 | 12.63 | -1.40 | -31.89 | -24.68 | -21.56 | 10.58 |
Eisosomes | 2.32 | 3.27 | 3.44 | -0.01 | 0.24 | 4.84 | 7.77 | 8.22 | 4.97 | 1.21 | 5.15 | 6.91 | 6.50 | 2.43 | -1.68 |
Endoplasmic Reticulum | -1.04 | -5.29 | -5.04 | -4.02 | 4.12 | 1.88 | -5.12 | -4.87 | -6.46 | 3.27 | 0.74 | -7.12 | -6.03 | -7.12 | 3.98 |
Endosome | -3.74 | -2.59 | -4.03 | 2.23 | -0.17 | -4.97 | -7.03 | -2.14 | 3.72 | 6.32 | -11.20 | -13.25 | -6.90 | 5.91 | 7.51 |
Golgi | 4.60 | 47.83* | 52.80* | 10.43 | -3.44 | -5.05 | 32.45 | 33.06 | 51.17* | 1.15 | -2.34 | 56.10* | 60.78* | 57.52* | 2.62 |
Lipid Particles | 4.33 | -1.64 | 8.44 | 0.15 | 7.70 | 6.15 | 6.79 | 10.04 | 6.64 | 9.01 | 6.49 | 6.57 | 13.68 | 5.90 | 10.58 |
Mitochondria | 4.67 | 20.56 | 20.14 | 2.44 | -2.17 | 5.78 | 13.53 | 11.04 | 7.63 | -6.15 | 7.59 | 22.18 | 20.04 | 10.47 | -4.36 |
Mitotic Spindle | -2.27 | -14.30 | -16.34 | -6.85 | 8.62 | 0.71 | -22.56* | -27.87* | -28.99* | -3.80 | -0.77 | -27.17 | -32.86 | -32.31* | 1.81 |
None | 5.49 | 2.23 | 3.53 | -4.41 | 0.75 | 2.45 | 2.13 | 1.88 | -1.79 | -0.78 | 3.29 | 2.34 | 2.60 | -1.32 | -0.32 |
Nuclear Periphery | 0.77 | -5.98 | -2.71 | -2.81 | 1.20 | -0.04 | -6.41 | -6.22 | -4.94 | -1.19 | -0.54 | -9.84 | -6.05 | -4.54 | -0.47 |
Nuclear Periphery Foci | 0.65 | -4.92 | -4.26 | -4.36 | 4.15 | 0.47 | -4.37 | -3.44 | -3.32 | 3.42 | -0.34 | -6.54 | -5.62 | -4.99 | 4.71 |
Nucleolus | 1.44 | -1.26 | 0.09 | -3.86 | 1.65 | 3.23 | -0.19 | 1.32 | -6.11 | 1.35 | 2.76 | -1.35 | 0.35 | -6.84 | 1.78 |
Nucleus | 1.37 | -3.26 | -5.11 | -5.22 | 1.28 | 2.43 | -0.90 | -4.12 | -5.38 | -3.57 | 1.41 | -4.13 | -6.77 | -7.10 | -2.94 |
Peroxisomes | -0.52 | -3.02 | -12.11 | -4.30 | -4.48 | 6.53 | -2.06 | -3.79 | -12.22 | -1.80 | 9.48 | -2.31 | -9.95 | -18.92 | -6.69 |
Vacuole | -1.21 | -6.91 | -16.88 | -16.20 | -5.46 | -3.64 | -14.46 | -22.28 | -21.52 | -8.87 | -3.74 | -15.98 | -27.86 | -26.90 | -11.17 |
Vacuole Periphery | -1.03 | 1.91 | -0.05 | 0.97 | -1.20 | 0.34 | 2.30 | -2.06 | -2.99 | -5.28 | -2.18 | 1.49 | -3.23 | -1.28 | -4.46 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|