Standard name | |
---|---|
Human Ortholog | |
Description | Rho GTPase activating protein (RhoGAP); involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence; potential GAP for Rho4p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 19 | 6 | 37 | 29 | 64 | 47 | 32 | 27 | 14 | 12 | 10 | 9 | 16 | 31 | 26 | 12 | 6 | 3 | 11 | 10 | 25 | 36 | 34 | 22 |
Bud | 1 | 5 | 59 | 40 | 47 | 71 | 54 | 54 | 23 | 48 | 33 | 57 | 6 | 11 | 8 | 3 | 1 | 1 | 2 | 1 | 5 | 13 | 5 | 4 |
Bud Neck | 0 | 0 | 8 | 1 | 9 | 5 | 4 | 3 | 5 | 2 | 5 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 17 | 22 | 66 | 45 | 17 | 9 |
Bud Site | 0 | 0 | 7 | 18 | 16 | 23 | 11 | 8 | 8 | 6 | 2 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 78 | 67 | 134 | 104 | 78 | 100 | 124 | 79 | 79 | 86 | 97 | 87 | 133 | 157 | 173 | 89 | 54 | 78 | 46 | 30 | 129 | 37 | 71 | 47 |
Cytoplasm | 88 | 123 | 388 | 250 | 300 | 363 | 428 | 397 | 225 | 344 | 275 | 289 | 199 | 187 | 214 | 85 | 84 | 85 | 71 | 100 | 218 | 93 | 141 | 175 |
Endoplasmic Reticulum | 6 | 6 | 2 | 0 | 17 | 7 | 14 | 9 | 17 | 3 | 3 | 2 | 1 | 5 | 7 | 3 | 3 | 5 | 8 | 1 | 22 | 14 | 31 | 20 |
Endosome | 0 | 0 | 1 | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 4 | 0 | 1 | 2 | 1 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 1 | 1 | 0 | 0 | 0 | 2 | 1 |
Mitochondria | 1 | 2 | 9 | 42 | 47 | 114 | 154 | 197 | 138 | 215 | 188 | 222 | 0 | 1 | 6 | 2 | 1 | 1 | 1 | 2 | 28 | 2 | 3 | 1 |
Nucleus | 0 | 0 | 1 | 1 | 2 | 2 | 3 | 8 | 1 | 2 | 3 | 0 | 3 | 1 | 3 | 8 | 5 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 6 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 3 | 0 | 9 | 8 | 18 | 11 | 7 | 7 | 4 | 6 | 4 | 6 | 1 | 0 | 1 | 3 | 6 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 3 | 3 | 11 | 7 | 10 | 31 | 4 | 15 | 10 | 15 | 0 | 0 | 0 | 4 | 13 | 11 | 0 | 1 | 11 | 3 | 1 | 1 |
Unique Cell Count | 140 | 150 | 472 | 338 | 463 | 538 | 648 | 631 | 377 | 522 | 445 | 500 | 242 | 272 | 291 | 142 | 128 | 127 | 168 | 174 | 518 | 254 | 313 | 289 |
Labelled Cell Count | 196 | 210 | 659 | 498 | 615 | 750 | 843 | 827 | 518 | 740 | 634 | 701 | 360 | 393 | 438 | 213 | 174 | 192 | 168 | 174 | 518 | 254 | 313 | 289 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.3 | 9.1 | 5.8 | 5.4 | 5.6 | 5.1 | 4.8 | 4.5 | 4.2 | 4.3 | 4.0 | 3.9 | 8.5 | 8.5 | 7.5 | 10.1 | 11.2 | 11.6 | 9.6 | 10.5 | 11.1 |
Std Deviation (1e-4) | 1.5 | 1.4 | 0.9 | 0.9 | 0.7 | 0.9 | 0.9 | 0.8 | 0.8 | 1.1 | 0.7 | 0.9 | 1.1 | 1.1 | 0.9 | 1.5 | 2.2 | 2.4 | 1.4 | 1.5 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.06 | -0.18 | -0.28 | -0.36 | -0.45 | -0.43 | -0.52 | -0.55 | 0.55 | 0.55 | 0.37 | 0.8 | 0.95 | 1.0 | 0.73 | 0.86 | 0.93 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.4 | 2.9 | 0.5 | 0 | 0 | 0 | 0 | 0 | 0 | -0.6 | 1.6 | 0.5 | 0.2 | -1.2 | -2.2 |
Bud | -0.3 | -1.1 | 0.3 | 0 | 0 | 0 | 0 | 0 | 0 | -4.4 | -3.8 | -4.6 | -3.6 | -3.9 | -3.9 |
Bud Neck | 0 | 0.3 | -1.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 3.1 | 1.9 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.7 | -4.2 | -3.7 | 0 | 0 | 0 | 0 | 0 | 0 | 6.9 | 7.9 | 8.5 | 7.4 | 3.0 | 6.9 |
Cytoplasm | -2.8 | -6.0 | -5.3 | -6.0 | -7.0 | -7.3 | -5.8 | -6.9 | -8.3 | 0 | -4.2 | -2.8 | -5.6 | -4.1 | -3.7 |
Endoplasmic Reticulum | 0 | 3.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 6.1 | 5.3 | 9.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.1 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0.5 | 1.8 | 0.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 2.2 | 0 | 1.4 | 4.1 | 0 | 2.6 | 2.1 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.3164 | 5.9958 | 5.0012 | 5.0337 | 5.7005 | 5.7787 | 6.9278 | 8.5661 | 7.6399 | 7.6476 | 7.7155 | 7.9534 | 8.8443 | 9.7448 | 9.3943 | 8.7344 | 9.2925 | 9.7769 |
Actin | 0.065 | 0.0094 | 0.0435 | 0.0296 | 0.0119 | 0.0093 | 0.0173 | 0.0185 | 0.0467 | 0.057 | 0.0024 | 0.0054 | 0.0628 | 0.0223 | 0.0354 | 0.0236 | 0.0624 | 0.0138 |
Bud | 0.0009 | 0.0006 | 0.0051 | 0.0013 | 0.0008 | 0.0009 | 0.0007 | 0.0037 | 0.0076 | 0.0016 | 0.0006 | 0.0015 | 0.0032 | 0.0049 | 0.0052 | 0.002 | 0.0026 | 0.0035 |
Bud Neck | 0.0128 | 0.0053 | 0.051 | 0.0441 | 0.2243 | 0.2494 | 0.0144 | 0.0122 | 0.0548 | 0.0887 | 0.2458 | 0.2084 | 0.021 | 0.009 | 0.0355 | 0.1177 | 0.239 | 0.2251 |
Bud Periphery | 0.0026 | 0.001 | 0.0141 | 0.0114 | 0.0054 | 0.0044 | 0.0031 | 0.004 | 0.0219 | 0.0039 | 0.0042 | 0.0053 | 0.0102 | 0.0071 | 0.0216 | 0.0135 | 0.0199 | 0.0128 |
Bud Site | 0.0238 | 0.1007 | 0.0558 | 0.0023 | 0.0023 | 0.0033 | 0.0532 | 0.247 | 0.1039 | 0.0137 | 0.008 | 0.0032 | 0.0855 | 0.2104 | 0.1079 | 0.0097 | 0.009 | 0.0039 |
Cell Periphery | 0.0975 | 0.0519 | 0.0874 | 0.1495 | 0.1038 | 0.0456 | 0.1277 | 0.0742 | 0.1369 | 0.1432 | 0.1393 | 0.085 | 0.2272 | 0.0962 | 0.1791 | 0.2102 | 0.1117 | 0.1088 |
Cytoplasm | 0.639 | 0.7888 | 0.6402 | 0.7461 | 0.6338 | 0.6609 | 0.6028 | 0.5976 | 0.5489 | 0.624 | 0.5306 | 0.6755 | 0.5287 | 0.6293 | 0.5942 | 0.5182 | 0.477 | 0.6119 |
Cytoplasmic Foci | 0.0112 | 0.0029 | 0.0149 | 0.002 | 0.0011 | 0.0036 | 0.0138 | 0.003 | 0.0209 | 0.0076 | 0.0027 | 0.0011 | 0.0138 | 0.0034 | 0.0018 | 0.0152 | 0.0163 | 0.0027 |
Eisosomes | 0.0022 | 0.0004 | 0.0007 | 0.0002 | 0.0001 | 0.0002 | 0.0024 | 0.0002 | 0.0004 | 0.0003 | 0 | 0.0001 | 0.0007 | 0.0005 | 0.0005 | 0.0003 | 0.0007 | 0.0003 |
Endoplasmic Reticulum | 0.0234 | 0.0083 | 0.0049 | 0.0035 | 0.01 | 0.0066 | 0.018 | 0.0085 | 0.0042 | 0.0121 | 0.0016 | 0.0045 | 0.0061 | 0.0026 | 0.0042 | 0.0043 | 0.0026 | 0.0013 |
Endosome | 0.0092 | 0.0008 | 0.0102 | 0.0002 | 0.0002 | 0.0018 | 0.0053 | 0.006 | 0.0135 | 0.0105 | 0.0493 | 0.0002 | 0.0056 | 0.001 | 0.0007 | 0.0261 | 0.0145 | 0.0037 |
Golgi | 0.0145 | 0.001 | 0.0137 | 0.0009 | 0.0005 | 0.0027 | 0.0035 | 0.0011 | 0.0086 | 0.0082 | 0.0033 | 0.0004 | 0.0078 | 0.0022 | 0.0015 | 0.0246 | 0.02 | 0.0041 |
Lipid Particles | 0.0118 | 0.0033 | 0.0125 | 0.0015 | 0.0015 | 0.0026 | 0.0101 | 0.0027 | 0.0058 | 0.0034 | 0.0007 | 0.0006 | 0.0047 | 0.0015 | 0.0006 | 0.0122 | 0.0018 | 0.0003 |
Mitochondria | 0.0167 | 0 | 0.0058 | 0 | 0 | 0.0026 | 0.0162 | 0.0001 | 0.0009 | 0.0032 | 0.0014 | 0 | 0.0054 | 0.0004 | 0.0071 | 0.0119 | 0.0149 | 0.0023 |
None | 0.0279 | 0.0224 | 0.0219 | 0.0035 | 0.0005 | 0.0009 | 0.0795 | 0.0125 | 0.0142 | 0.0022 | 0.0003 | 0.0068 | 0.0024 | 0.0012 | 0.0013 | 0.0011 | 0.0007 | 0.0033 |
Nuclear Periphery | 0.0039 | 0.0004 | 0.0011 | 0.0002 | 0.0003 | 0.0002 | 0.0062 | 0.0006 | 0.0006 | 0.0018 | 0.003 | 0.0001 | 0.0005 | 0.0003 | 0.0002 | 0.0004 | 0.0002 | 0.0001 |
Nucleolus | 0.0005 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 |
Nucleus | 0.0102 | 0.0005 | 0.0009 | 0.0004 | 0.0004 | 0.0005 | 0.0023 | 0.0009 | 0.0009 | 0.0012 | 0.0006 | 0.0005 | 0.0008 | 0.0008 | 0.0005 | 0.0007 | 0.0004 | 0.0004 |
Peroxisomes | 0.0084 | 0.0001 | 0.0094 | 0.0001 | 0 | 0.003 | 0.0134 | 0.0001 | 0.0034 | 0.0125 | 0.0002 | 0 | 0.0053 | 0.0012 | 0.0002 | 0.0038 | 0.0044 | 0.0002 |
Punctate Nuclear | 0.0145 | 0.0003 | 0.0017 | 0.0001 | 0.0001 | 0.0002 | 0.0028 | 0.0003 | 0.0013 | 0.001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0005 | 0.0003 | 0.0001 |
Vacuole | 0.0036 | 0.0016 | 0.0039 | 0.0029 | 0.0028 | 0.0013 | 0.0066 | 0.0065 | 0.0039 | 0.0035 | 0.0029 | 0.0013 | 0.0075 | 0.0052 | 0.0023 | 0.0033 | 0.0014 | 0.0013 |
Vacuole Periphery | 0.0005 | 0 | 0.0004 | 0 | 0 | 0.0001 | 0.0004 | 0.0002 | 0.0003 | 0.0003 | 0.0026 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0006 | 0.0002 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 49.1137 | 35.9427 | 40.8505 | 61.0882 | 47.5111 | 38.1501 | 51.7523 | 54.438 | 45.1363 | 71.5783 |
Translational Efficiency | 0.4893 | 0.509 | 0.4277 | 0.2943 | 0.4043 | 0.5754 | 0.3938 | 0.2806 | 0.4137 | 0.3528 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
711 | 577 | 898 | 1230 | 1355 | 1767 | 1709 | 2888 | 2066 | 2344 | 2607 | 4118 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 790.08 | 1001.36 | 1348.96 | 1079.96 | 865.16 | 963.90 | 1106.19 | 1146.19 | 839.32 | 973.12 | 1189.81 | 1126.41 |
Standard Deviation | 117.30 | 137.07 | 170.61 | 151.45 | 109.38 | 137.13 | 142.18 | 157.55 | 117.71 | 138.06 | 191.28 | 158.67 |
Intensity Change Log 2 | — | 0.341890 | 0.771777 | 0.450907 | — | 0.155917 | 0.354560 | 0.405807 | — | 0.247680 | 0.568771 | 0.427510 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.012831 | 0.010971 | 0.030404 | 0.022379 | 0.007427 | 0.010717 | 0.038070 | 0.013404 | 0.009286 | 0.010779 | 0.035430 | 0.016085 |
Bud Neck | 0.085550 | 0.046540 | 0.018332 | 0.018268 | 0.075470 | 0.064491 | 0.049828 | 0.032912 | 0.078939 | 0.060073 | 0.038979 | 0.028538 |
Bud Site | 0.208073* | 0.257517* | 0.222966* | 0.230168* | 0.201903* | 0.222146* | 0.286449* | 0.175019* | 0.204026* | 0.230853* | 0.264582* | 0.191491* |
Cell Periphery | 0.033325 | 0.021170 | 0.008922 | 0.003160 | 0.020841 | 0.009869 | 0.010832 | 0.002686 | 0.025137 | 0.012651 | 0.010174 | 0.002827 |
Cytoplasm | 0.546619* | 0.534796* | 0.522940* | 0.567627* | 0.617243* | 0.601576* | 0.477389* | 0.716358* | 0.592938* | 0.585138* | 0.493079* | 0.671934* |
Cytoplasmic Foci | 0.035685 | 0.058636 | 0.058714 | 0.025393 | 0.029465 | 0.041286 | 0.024590 | 0.006260 | 0.031606 | 0.045557 | 0.036344 | 0.011975 |
Eisosomes | 0.005221 | 0.001379 | 0.000565 | 0.000468 | 0.002676 | 0.001349 | 0.001651 | 0.000320 | 0.003552 | 0.001357 | 0.001276 | 0.000364 |
Endoplasmic Reticulum | 0.007605 | 0.003621 | 0.001641 | 0.000616 | 0.003076 | 0.001210 | 0.000861 | 0.000062 | 0.004635 | 0.001804 | 0.001130 | 0.000228 |
Endosome | 0.006446 | 0.008342 | 0.002224 | 0.005043 | 0.003314 | 0.005800 | 0.001861 | 0.001116 | 0.004391 | 0.006426 | 0.001986 | 0.002289 |
Golgi | 0.006311 | 0.009215 | 0.001083 | 0.005060 | 0.006499 | 0.008765 | 0.000570 | 0.000706 | 0.006434 | 0.008876 | 0.000747 | 0.002007 |
Lipid Particles | 0.002861 | 0.003287 | 0.012319 | 0.001057 | 0.002228 | 0.001789 | 0.001990 | 0.000230 | 0.002446 | 0.002158 | 0.005548 | 0.000477 |
Mitochondria | 0.005744 | 0.002212 | 0.000041 | 0.001494 | 0.002132 | 0.002016 | 0.000321 | 0.001507 | 0.003375 | 0.002064 | 0.000225 | 0.001503 |
Mitotic Spindle | 0.000668 | 0.002623 | 0.000349 | 0.011795 | 0.000193 | 0.000347 | 0.000382 | 0.003204 | 0.000356 | 0.000908 | 0.000371 | 0.005770 |
None | 0.001663 | 0.000164 | 0.000325 | 0.000200 | 0.001394 | 0.000208 | 0.000191 | 0.000267 | 0.001487 | 0.000197 | 0.000237 | 0.000247 |
Nuclear Periphery | 0.000085 | 0.000018 | 0.000049 | 0.000098 | 0.000290 | 0.000011 | 0.000024 | 0.000014 | 0.000220 | 0.000013 | 0.000032 | 0.000039 |
Nuclear Periphery Foci | 0.000810 | 0.001133 | 0.006420 | 0.000721 | 0.000569 | 0.000265 | 0.001180 | 0.000079 | 0.000652 | 0.000478 | 0.002985 | 0.000271 |
Nucleolus | 0.000562 | 0.000081 | 0.000035 | 0.000040 | 0.000145 | 0.000217 | 0.000035 | 0.000044 | 0.000288 | 0.000184 | 0.000035 | 0.000043 |
Nucleus | 0.010314 | 0.002840 | 0.013601 | 0.004935 | 0.003815 | 0.002727 | 0.009025 | 0.004668 | 0.006052 | 0.002755 | 0.010601 | 0.004748 |
Peroxisomes | 0.003137 | 0.003727 | 0.002040 | 0.003262 | 0.002299 | 0.003777 | 0.002495 | 0.001650 | 0.002587 | 0.003765 | 0.002339 | 0.002131 |
Vacuole | 0.024080 | 0.030392 | 0.096902 | 0.096900 | 0.016624 | 0.019966 | 0.092140 | 0.039286 | 0.019190 | 0.022532 | 0.093780 | 0.056494 |
Vacuole Periphery | 0.002409 | 0.001335 | 0.000127 | 0.001317 | 0.002396 | 0.001467 | 0.000116 | 0.000210 | 0.002401 | 0.001434 | 0.000120 | 0.000540 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.02 | -6.08 | -2.39 | -4.40 | 4.20 | -3.69 | -16.23 | -5.91 | -1.70 | 12.53 | -1.66 | -16.59 | -5.24 | -3.74 | 12.98 |
Bud Neck | 5.65 | 11.72 | 12.00 | 8.13 | 1.27 | 2.33 | 6.77 | 11.27 | 10.83 | 5.69 | 4.79 | 12.23 | 15.97 | 13.48 | 5.30 |
Bud Site | -3.93 | 0.77 | 1.95 | 6.04 | 1.31 | -2.64 | -7.44 | 5.76 | 9.82 | 14.26* | -4.12 | -5.84 | 5.97 | 11.23 | 12.80 |
Cell Periphery | 3.46 | 9.01 | 11.01 | 8.47 | 8.54 | 7.96 | 7.53 | 13.99 | 13.63 | 10.76 | 8.68 | 11.66 | 17.45 | 14.84 | 13.36 |
Cytoplasm | 0.84 | 2.52 | -0.63 | -1.57 | -3.57 | 1.63 | 13.89 | -11.83 | -15.11 | -29.36 | 0.98 | 12.64 | -10.46 | -12.35 | -26.21 |
Cytoplasmic Foci | -4.74 | -8.02 | 2.26 | 6.56 | 11.86 | -4.50 | 1.32 | 13.08 | 15.89 | 13.84 | -6.05 | -4.23 | 11.99 | 16.00 | 18.40 |
Eisosomes | 6.63 | 8.23 | 8.46 | 8.53 | 2.22 | 5.87 | 4.76 | 11.27 | 10.20 | 9.99 | 8.75 | 9.35 | 13.34 | 12.56 | 10.16 |
Endoplasmic Reticulum | 2.09 | 3.75 | 4.44 | 2.90 | 5.60 | 3.07 | 3.84 | 5.32 | 5.24 | 9.84 | 3.88 | 5.25 | 6.68 | 5.14 | 11.14 |
Endosome | -1.45 | 4.59 | 2.00 | 3.39 | -2.93 | -4.14 | 4.28 | 6.82 | 7.96 | 2.39 | -3.44 | 6.20 | 5.53 | 8.05 | -0.09 |
Golgi | -3.61 | 14.07 | 2.12 | 4.04 | -2.40 | -2.71 | 13.70 | 12.72 | 11.08 | -0.76 | -3.77 | 18.31 | 10.18 | 10.86 | -2.46 |
Lipid Particles | -0.43 | -9.62 | 3.11 | 3.69 | 13.75 | 1.40 | 0.40 | 7.20 | 10.61 | 9.56 | 0.97 | -7.74 | 7.25 | 8.95 | 15.82 |
Mitochondria | 1.85 | 4.73 | 3.52 | 0.69 | -2.00 | 0.15 | 2.97 | 1.11 | 1.14 | -2.57 | 1.75 | 5.45 | 3.26 | 1.34 | -3.28 |
Mitotic Spindle | -1.54 | 1.11 | -4.28 | -2.79 | -4.46 | -1.17 | -0.98 | -4.13 | -3.87 | -3.74 | -1.64 | -0.10 | -5.85 | -4.72 | -5.81 |
None | 2.00 | 1.85 | 1.97 | -0.32 | 1.00 | 1.57 | 1.60 | 1.50 | -0.48 | -0.69 | 2.32 | 2.28 | 2.24 | -0.55 | -0.21 |
Nuclear Periphery | 1.90 | 0.68 | -0.37 | -2.84 | -1.39 | 1.14 | 1.07 | 1.12 | -3.05 | 4.10 | 1.29 | 1.12 | 1.10 | -3.36 | -0.28 |
Nuclear Periphery Foci | -0.82 | -9.59 | 0.18 | 1.35 | 14.21 | 1.41 | -3.22 | 2.24 | 6.25 | 14.16 | 0.49 | -9.59 | 1.70 | 2.08 | 17.50 |
Nucleolus | 1.44 | 1.76 | 1.73 | 1.52 | -1.48 | -0.54 | 2.32 | 2.07 | 1.30 | -2.04 | 0.71 | 2.29 | 2.20 | 1.50 | -2.52 |
Nucleus | 4.33 | -3.65 | 2.46 | -5.68 | 9.05 | 2.62 | -10.92 | -3.88 | -10.91 | 9.63 | 5.12 | -8.30 | 0.50 | -12.35 | 12.91 |
Peroxisomes | -1.27 | 4.53 | 0.60 | 1.77 | -3.68 | -3.82 | -0.28 | 4.17 | 5.53 | 2.61 | -3.70 | 1.89 | 3.52 | 5.55 | 0.87 |
Vacuole | -2.22 | -19.78 | -21.72 | -19.59 | -4.36 | -2.62 | -23.30 | -19.18 | -15.86 | 13.72 | -2.74 | -30.29 | -27.05 | -24.46 | 8.59 |
Vacuole Periphery | 2.40 | 6.01 | 2.84 | 0.84 | -2.82 | 1.14 | 2.88 | 2.76 | 6.14 | -2.04 | 1.72 | 4.26 | 3.59 | 5.22 | -3.31 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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