Standard name | |
---|---|
Human Ortholog | |
Description | Biotin synthase; catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 8 | 6 | 6 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 3 | 1 | 1 | 0 | 1 | 1 | 3 | 5 | 7 | 2 | 4 | 11 | 14 |
Bud | 3 | 4 | 14 | 9 | 10 | 13 | 34 | 28 | 12 | 12 | 16 | 14 | 0 | 2 | 0 | 1 | 3 | 8 | 2 | 1 | 0 | 2 | 4 | 7 |
Bud Neck | 1 | 0 | 3 | 0 | 4 | 1 | 2 | 7 | 1 | 8 | 1 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 4 |
Bud Site | 0 | 2 | 3 | 1 | 0 | 0 | 1 | 8 | 9 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | – | – | – | – | – | – |
Cell Periphery | 0 | 19 | 6 | 10 | 4 | 3 | 5 | 3 | 2 | 0 | 1 | 3 | 2 | 1 | 1 | 8 | 4 | 12 | 0 | 0 | 1 | 0 | 1 | 1 |
Cytoplasm | 23 | 7 | 20 | 32 | 44 | 59 | 74 | 98 | 91 | 114 | 71 | 54 | 113 | 168 | 162 | 1 | 3 | 10 | 5 | 0 | 4 | 20 | 34 | 50 |
Endoplasmic Reticulum | 3 | 9 | 4 | 3 | 5 | 2 | 0 | 1 | 1 | 2 | 0 | 0 | 3 | 1 | 0 | 2 | 0 | 5 | 7 | 1 | 1 | 1 | 3 | 1 |
Endosome | 0 | 27 | 14 | 7 | 28 | 4 | 4 | 4 | 0 | 1 | 0 | 0 | 2 | 8 | 2 | 8 | 5 | 9 | 13 | 14 | 17 | 10 | 16 | 18 |
Golgi | 1 | 12 | 2 | 2 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 17 | 1 | 14 | 6 | 18 | 4 | 2 | 1 | 2 |
Mitochondria | 108 | 208 | 180 | 153 | 124 | 235 | 273 | 249 | 217 | 210 | 178 | 143 | 8 | 2 | 1 | 10 | 1 | 6 | 118 | 215 | 258 | 116 | 145 | 145 |
Nucleus | 0 | 1 | 5 | 0 | 5 | 2 | 6 | 5 | 5 | 3 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 6 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 9 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 1 | 3 | 3 | 1 | 0 |
Nucleolus | 2 | 4 | 0 | 0 | 1 | 2 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 1 | 1 | 1 | 1 | 1 |
Peroxisomes | 1 | 22 | 22 | 22 | 20 | 3 | 3 | 7 | 2 | 3 | 1 | 0 | 1 | 0 | 5 | 7 | 6 | 19 | 4 | 11 | 12 | 5 | 4 | 2 |
SpindlePole | 2 | 6 | 34 | 20 | 29 | 20 | 24 | 40 | 17 | 23 | 2 | 14 | 0 | 2 | 0 | 7 | 3 | 7 | 4 | 1 | 2 | 5 | 8 | 8 |
Vac/Vac Membrane | 5 | 33 | 16 | 0 | 18 | 12 | 9 | 10 | 8 | 6 | 8 | 8 | 0 | 5 | 2 | 12 | 3 | 23 | 7 | 12 | 3 | 11 | 9 | 11 |
Unique Cell Count | 131 | 257 | 243 | 207 | 232 | 291 | 342 | 365 | 288 | 309 | 229 | 188 | 131 | 184 | 171 | 60 | 24 | 105 | 186 | 291 | 321 | 191 | 257 | 278 |
Labelled Cell Count | 150 | 371 | 329 | 265 | 295 | 356 | 439 | 464 | 369 | 384 | 282 | 240 | 135 | 191 | 174 | 77 | 32 | 130 | 186 | 291 | 321 | 191 | 257 | 278 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.7 | 6.6 | 5.3 | 4.9 | 5.2 | 4.0 | 3.7 | 4.2 | 3.5 | 3.5 | 3.2 | 3.5 | 4.9 | 5.3 | 5.3 | 7.9 | 7.2 | 7.3 | 5.1 | 5.2 | 5.4 |
Std Deviation (1e-4) | 0.7 | 3.2 | 1.2 | 1.6 | 1.8 | 1.2 | 1.5 | 1.9 | 1.5 | 1.8 | 1.1 | 1.0 | 1.4 | 1.4 | 1.9 | 2.2 | 2.1 | 2.3 | 1.9 | 2.3 | 2.7 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.02 | -0.4 | -0.53 | -0.32 | -0.59 | -0.58 | -0.7 | -0.58 | -0.1 | 0.01 | 0.02 | 0.58 | 0.46 | 0.46 | -0.05 | -0.01 | 0.03 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | -0.7 | -0.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.5 | -3.2 | 0 | 0 | 0.7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 |
Cytoplasm | 2.4 | 3.4 | 3.9 | 4.4 | 5.7 | 6.6 | 7.8 | 6.3 | 5.6 | 15.0 | 17.1 | 17.5 | 0 | 0 | 0.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -1.2 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.9 | -0.7 | -2.4 | 0 | 0 | 1.0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | -4.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -12.5 | -15.1 | -14.8 | -8.2 | -7.0 | -11.7 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0.6 | -0.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.2 | -4.2 | -2.5 | 0.6 | 0 | 2.4 |
SpindlePole | -1.4 | -0.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -4.5 | -4.8 | -5.1 | -0.5 | 0 | -1.9 |
Vacuole | -3.8 | 0.5 | -1.3 | -2.3 | -2.3 | -2.1 | -2.8 | -1.5 | -1.0 | -3.0 | -1.8 | -2.7 | 3.2 | 0 | 4.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.8971 | 6.7119 | 5.7962 | 5.2639 | 5.3491 | 5.8873 | 4.7415 | 4.0984 | 4.9708 | 3.825 | 3.4703 | 4.2162 | 6.2311 | 6.6082 | 6.024 | 5.661 | 5.4292 | 5.4129 |
Actin | 0.0268 | 0.0067 | 0.0097 | 0.0103 | 0.0178 | 0.0098 | 0.0206 | 0.0069 | 0.0103 | 0.0013 | 0.0015 | 0.0026 | 0.0502 | 0.0093 | 0.0318 | 0.0047 | 0.0018 | 0.0105 |
Bud | 0.0004 | 0.0007 | 0.0001 | 0.0024 | 0.0003 | 0.0003 | 0.0013 | 0.0021 | 0.0015 | 0.0001 | 0.0003 | 0.0002 | 0.0009 | 0.0009 | 0.0004 | 0.0012 | 0.0001 | 0.0001 |
Bud Neck | 0.0021 | 0.0003 | 0.0001 | 0.0007 | 0.0004 | 0.0002 | 0.0014 | 0.0006 | 0.0005 | 0.0002 | 0.0003 | 0.0003 | 0.0008 | 0.0004 | 0.0022 | 0.0005 | 0.0002 | 0.0002 |
Bud Periphery | 0.0007 | 0.0009 | 0.0003 | 0.0097 | 0.0006 | 0.0005 | 0.0032 | 0.0034 | 0.0021 | 0.0004 | 0.0009 | 0.0009 | 0.0013 | 0.0014 | 0.0006 | 0.0048 | 0.0003 | 0.0004 |
Bud Site | 0.0058 | 0.0089 | 0.0006 | 0.0018 | 0.001 | 0.0002 | 0.0045 | 0.0146 | 0.0142 | 0.0004 | 0.0002 | 0.0003 | 0.0029 | 0.0039 | 0.0013 | 0.0018 | 0.0003 | 0.0002 |
Cell Periphery | 0.0005 | 0.0002 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0007 | 0.0008 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0.0001 | 0.002 | 0.0001 | 0.0001 |
Cytoplasm | 0.0008 | 0.0005 | 0.0003 | 0.0018 | 0.0003 | 0.0001 | 0.0029 | 0.0014 | 0.0008 | 0.0001 | 0.0016 | 0.0011 | 0.0003 | 0.0006 | 0.0005 | 0.0002 | 0.0001 | 0.0001 |
Cytoplasmic Foci | 0.0151 | 0.0098 | 0.003 | 0.0052 | 0.0101 | 0.0083 | 0.0192 | 0.0142 | 0.0295 | 0.013 | 0.0218 | 0.0177 | 0.0062 | 0.0063 | 0.0124 | 0.0043 | 0.0051 | 0.0024 |
Eisosomes | 0.0014 | 0.0002 | 0.0019 | 0.0002 | 0.0003 | 0.0005 | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0006 | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.0014 | 0.0001 | 0.0006 | 0.0007 | 0.0002 | 0.0001 | 0.0005 | 0.0004 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0005 | 0.0003 | 0.0004 | 0.0001 | 0.0002 | 0.0002 |
Endosome | 0.0232 | 0.023 | 0.0063 | 0.0173 | 0.0062 | 0.0072 | 0.0307 | 0.0215 | 0.019 | 0.0109 | 0.0108 | 0.0161 | 0.0181 | 0.021 | 0.019 | 0.0073 | 0.0278 | 0.0051 |
Golgi | 0.0404 | 0.0376 | 0.0127 | 0.0173 | 0.0172 | 0.0277 | 0.0227 | 0.0407 | 0.0182 | 0.0171 | 0.02 | 0.0278 | 0.0346 | 0.0428 | 0.0344 | 0.0104 | 0.0305 | 0.0219 |
Lipid Particles | 0.0316 | 0.0035 | 0.0103 | 0.0108 | 0.0057 | 0.0108 | 0.0258 | 0.0104 | 0.0068 | 0.0141 | 0.0048 | 0.0139 | 0.0319 | 0.0057 | 0.0092 | 0.0077 | 0.0173 | 0.0085 |
Mitochondria | 0.8023 | 0.8789 | 0.9301 | 0.8883 | 0.8687 | 0.9109 | 0.7918 | 0.8278 | 0.8267 | 0.8912 | 0.901 | 0.8683 | 0.8113 | 0.881 | 0.8686 | 0.9265 | 0.8713 | 0.9263 |
None | 0.0027 | 0.0001 | 0.0002 | 0.0006 | 0.0007 | 0.0001 | 0.0101 | 0.0013 | 0.0009 | 0.0001 | 0.0217 | 0.0071 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.0021 | 0.0001 | 0.0001 | 0.0014 | 0.0018 | 0 | 0.0014 | 0.0013 | 0.0002 | 0 | 0.0002 | 0 | 0.0063 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0002 |
Nucleolus | 0.0011 | 0.0001 | 0 | 0.0003 | 0.0014 | 0 | 0.001 | 0.0013 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0009 | 0.0003 | 0.0001 | 0.0009 | 0.0002 | 0.0001 |
Nucleus | 0.0005 | 0.0001 | 0.0001 | 0.0005 | 0.001 | 0 | 0.0006 | 0.0004 | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0009 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 |
Peroxisomes | 0.0355 | 0.0231 | 0.0215 | 0.0241 | 0.0444 | 0.0194 | 0.0503 | 0.0446 | 0.061 | 0.0495 | 0.0067 | 0.0407 | 0.019 | 0.0191 | 0.015 | 0.018 | 0.0393 | 0.0198 |
Punctate Nuclear | 0.0022 | 0.0001 | 0.0001 | 0.0008 | 0.0201 | 0.002 | 0.001 | 0.0002 | 0.0017 | 0.0001 | 0.001 | 0.0005 | 0.0005 | 0.0001 | 0.0006 | 0 | 0.0001 | 0 |
Vacuole | 0.0013 | 0.0018 | 0.0006 | 0.0034 | 0.0004 | 0.0005 | 0.0067 | 0.0037 | 0.0024 | 0.0004 | 0.0023 | 0.0007 | 0.003 | 0.0026 | 0.0012 | 0.0048 | 0.0018 | 0.0006 |
Vacuole Periphery | 0.0018 | 0.0035 | 0.0009 | 0.0022 | 0.0012 | 0.0012 | 0.003 | 0.0021 | 0.0019 | 0.0006 | 0.0045 | 0.0011 | 0.0089 | 0.0032 | 0.0018 | 0.004 | 0.003 | 0.0029 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 126.6763 | 35.8523 | 21.413 | 21.2675 | 30.4386 | 82.1229 | 22.1038 | 13.4267 | 15.419 | 17.2659 |
Translational Efficiency | 1.2382 | 1.2822 | 1.0077 | 1.2138 | 1.2752 | 1.6606 | 1.0403 | 1.4786 | 1.0375 | 1.1713 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
25 | 421 | 1529 | 318 | 1334 | 1844 | 2042 | 825 | 1359 | 2265 | 3571 | 1143 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 867.07 | 767.40 | 1638.62 | 980.04 | 618.44 | 687.19 | 1292.54 | 1014.82 | 623.01 | 702.10 | 1440.72 | 1005.14 |
Standard Deviation | 231.49 | 118.93 | 462.13 | 249.36 | 74.11 | 85.37 | 391.44 | 248.63 | 86.57 | 97.65 | 456.49 | 249.32 |
Intensity Change Log 2 | — | -0.176170 | 0.918261 | 0.176692 | — | 0.152075 | 1.063503 | 0.714518 | — | -0.030346 | 0.980513 | 0.425329 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.013474 | 0.002276 | 0.002747 | 0.023761 | 0.001377 | 0.001375 | 0.006524 | 0.045243 | 0.001600 | 0.001542 | 0.004907 | 0.039266 |
Bud Neck | 0.007178 | 0.015859 | 0.000549 | 0.015030 | 0.030392 | 0.049250 | 0.006653 | 0.013272 | 0.029965 | 0.043044 | 0.004040 | 0.013761 |
Bud Site | 0.088272 | 0.032638 | 0.002823 | 0.131703* | 0.019978 | 0.030935 | 0.040405 | 0.166986* | 0.021234 | 0.031252 | 0.024313 | 0.157169* |
Cell Periphery | 0.004092 | 0.000524 | 0.000111 | 0.001019 | 0.000408 | 0.000196 | 0.000544 | 0.001984 | 0.000476 | 0.000257 | 0.000359 | 0.001715 |
Cytoplasm | 0.122305* | 0.012867 | 0.000728 | 0.058226 | 0.085979 | 0.256984* | 0.015548 | 0.079464 | 0.086647 | 0.211610* | 0.009202 | 0.073555 |
Cytoplasmic Foci | 0.127114 | 0.110256 | 0.004519 | 0.075528 | 0.265249* | 0.292584* | 0.028388 | 0.057729 | 0.262707* | 0.258695* | 0.018168 | 0.062681 |
Eisosomes | 0.000601 | 0.000130 | 0.000447 | 0.000206 | 0.000336 | 0.000162 | 0.000206 | 0.000257 | 0.000340 | 0.000156 | 0.000309 | 0.000243 |
Endoplasmic Reticulum | 0.013023 | 0.000290 | 0.000038 | 0.002023 | 0.002904 | 0.000867 | 0.000558 | 0.001796 | 0.003090 | 0.000759 | 0.000336 | 0.001859 |
Endosome | 0.027282 | 0.108651 | 0.001335 | 0.062348 | 0.059464 | 0.041199 | 0.022294 | 0.036779 | 0.058872 | 0.053736 | 0.013320 | 0.043893 |
Golgi | 0.093794 | 0.126210* | 0.007738 | 0.069094 | 0.064519 | 0.023603 | 0.030118 | 0.074781 | 0.065058 | 0.042675 | 0.020536 | 0.073199 |
Lipid Particles | 0.006151 | 0.002319 | 0.001685 | 0.003879 | 0.025425 | 0.010484 | 0.003936 | 0.001341 | 0.025071 | 0.008967 | 0.002972 | 0.002047 |
Mitochondria | 0.243840* | 0.432439* | 0.964493* | 0.326721* | 0.297361* | 0.080924 | 0.741976* | 0.314640* | 0.296376* | 0.146261* | 0.837251* | 0.318001* |
Mitotic Spindle | 0.028355 | 0.028442 | 0.003001 | 0.063257 | 0.006295 | 0.017590 | 0.038087 | 0.057591 | 0.006701 | 0.019607 | 0.023064 | 0.059167 |
None | 0.000713 | 0.000369 | 0.001222 | 0.000696 | 0.001455 | 0.002335 | 0.001037 | 0.000664 | 0.001441 | 0.001969 | 0.001116 | 0.000673 |
Nuclear Periphery | 0.001106 | 0.000062 | 0.000110 | 0.000553 | 0.001010 | 0.000849 | 0.000640 | 0.000189 | 0.001012 | 0.000703 | 0.000413 | 0.000290 |
Nuclear Periphery Foci | 0.013023 | 0.000174 | 0.000054 | 0.001175 | 0.000428 | 0.000497 | 0.000509 | 0.000372 | 0.000659 | 0.000437 | 0.000314 | 0.000596 |
Nucleolus | 0.007366 | 0.002630 | 0.000163 | 0.000930 | 0.001871 | 0.002188 | 0.000783 | 0.000254 | 0.001972 | 0.002270 | 0.000518 | 0.000442 |
Nucleus | 0.073231 | 0.004509 | 0.000033 | 0.012083 | 0.020081 | 0.070158 | 0.002005 | 0.008807 | 0.021059 | 0.057955 | 0.001160 | 0.009718 |
Peroxisomes | 0.039818 | 0.094254 | 0.003773 | 0.052035 | 0.075308 | 0.039952 | 0.029779 | 0.083379 | 0.074655 | 0.050045 | 0.018644 | 0.074659 |
Vacuole | 0.069264 | 0.014439 | 0.001865 | 0.079016 | 0.029048 | 0.071120 | 0.019079 | 0.045864 | 0.029788 | 0.060585 | 0.011709 | 0.055088 |
Vacuole Periphery | 0.019999 | 0.010664 | 0.002563 | 0.020719 | 0.011113 | 0.006748 | 0.010934 | 0.008609 | 0.011276 | 0.007476 | 0.007350 | 0.011978 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.02 | 1.92 | -1.09 | -3.66 | -3.55 | 0.03 | -8.76 | -10.28 | -10.26 | -8.90 | 0.15 | -6.21 | -10.78 | -10.79 | -9.68 |
Bud Neck | -2.28 | 3.13 | -2.73 | 0.22 | -7.27 | -5.57 | 10.28 | 6.38 | 12.31 | -4.17 | -4.22 | 11.72 | 6.46 | 11.84 | -7.86 |
Bud Site | 1.54 | 2.41 | -0.93 | -7.02 | -10.11 | -4.20 | -7.14 | -17.36 | -15.97 | -14.14 | -3.89 | -1.82 | -18.82 | -17.39 | -18.29 |
Cell Periphery | 1.24 | 1.39 | 1.10 | -1.62 | -3.84 | 4.76 | -2.63 | -2.12 | -2.45 | -1.86 | 3.01 | 1.30 | -2.24 | -2.72 | -2.46 |
Cytoplasm | 2.21 | 2.46 | 0.97 | -6.68 | -8.20 | -23.26 | 15.07 | -1.74 | 17.53 | -11.86 | -18.45 | 17.75 | -0.81 | 15.75 | -14.88 |
Cytoplasmic Foci | 0.43 | 3.39 | 1.41 | 3.74 | -10.69 | -3.21 | 38.08 | 30.46 | 36.76 | -7.37 | 0.65 | 40.48 | 30.16 | 34.32 | -13.49 |
Eisosomes | 2.17 | 0.67 | 1.83 | -2.42 | 3.97 | 8.40 | 5.23 | 3.56 | -4.58 | -1.67 | 9.05 | 0.95 | 4.50 | -4.94 | 2.66 |
Endoplasmic Reticulum | 2.58 | 2.63 | 2.18 | -4.29 | -4.88 | 4.06 | 4.62 | 1.15 | -5.34 | -6.44 | 4.67 | 5.53 | 1.54 | -6.53 | -8.56 |
Endosome | -8.33 | 3.76 | -3.44 | 6.06 | -10.90 | 5.77 | 12.22 | 7.26 | 2.68 | -4.25 | 1.61 | 16.13 | 5.46 | 4.69 | -11.46 |
Golgi | -0.88 | 2.28 | 0.85 | 6.27 | -7.46 | 11.71 | 9.53 | -0.35 | -8.53 | -7.10 | 5.95 | 13.29 | 0.02 | -4.98 | -10.71 |
Lipid Particles | 1.12 | 1.31 | 0.55 | -1.50 | -2.10 | 7.48 | 11.42 | 12.93 | 10.54 | 4.58 | 8.41 | 12.15 | 12.41 | 8.70 | 1.35 |
Mitochondria | -3.03 | -11.92 | -0.69 | 5.45 | 31.97* | 22.25* | -37.06* | 1.07 | -14.95 | 30.40* | 14.57 | -53.22* | 0.99 | -11.18 | 45.97* |
Mitotic Spindle | -0.02 | 1.06 | -1.48 | -3.57 | -6.54 | -5.80 | -11.91 | -10.84 | -8.16 | -3.66 | -6.85 | -9.27 | -12.17 | -8.91 | -8.03 |
None | 1.24 | -1.79 | -0.09 | -2.65 | 3.31 | -1.86 | 1.22 | 2.01 | 4.69 | 1.94 | -1.22 | 1.03 | 2.06 | 4.45 | 3.42 |
Nuclear Periphery | 1.32 | 1.26 | 0.60 | -5.11 | -4.70 | 0.26 | 0.65 | 1.39 | 1.63 | 3.22 | 0.53 | 1.14 | 1.21 | 1.10 | 0.43 |
Nuclear Periphery Foci | 1.41 | 1.43 | 1.29 | -3.49 | -3.88 | -0.42 | -0.90 | -0.89 | -0.13 | 0.01 | 1.07 | 1.82 | -0.33 | -2.03 | -3.87 |
Nucleolus | 0.84 | 1.30 | 1.16 | 1.19 | -1.79 | -0.35 | 2.61 | 3.81 | 7.03 | 3.74 | -0.29 | 3.45 | 3.51 | 5.10 | 0.43 |
Nucleus | 2.02 | 2.16 | 1.47 | -3.85 | -5.20 | -14.63 | 9.79 | 0.48 | 12.88 | -5.73 | -12.02 | 10.84 | 0.39 | 11.17 | -7.85 |
Peroxisomes | -2.79 | 1.97 | -0.44 | 4.47 | -6.61 | 8.32 | 10.56 | -0.02 | -5.57 | -7.13 | 5.69 | 14.34 | 1.15 | -3.36 | -9.98 |
Vacuole | 2.29 | 2.80 | -1.94 | -9.10 | -10.27 | -13.04 | 3.37 | -8.15 | -1.27 | -9.54 | -10.65 | 8.24 | -10.83 | -5.25 | -14.30 |
Vacuole Periphery | 0.76 | 1.45 | 0.13 | -2.33 | -5.32 | 4.54 | 0.14 | 2.34 | -0.82 | 2.18 | 4.01 | 4.27 | 0.40 | -2.47 | -2.56 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|