Standard name | |
---|---|
Human Ortholog | |
Description | Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex; Cbp3p-Cbp6p complex is sequestered if assembly of Complex III is blocked, downregulating COB mRNA translation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 4 | 1 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 2 | 6 | 3 | 9 | 0 | 0 | 0 | 0 | 2 | 1 | 6 | 10 | 18 |
Bud | 2 | 0 | 3 | 6 | 10 | 8 | 13 | 10 | 15 | 21 | 22 | 14 | 4 | 5 | 2 | 2 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 1 | 0 | 0 | 0 | 5 | 3 | 1 | 4 | 3 | 4 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 10 | 2 | 7 | 1 | 0 | 3 | 1 | 3 | 2 | 3 | 1 | 5 | 6 | 14 | 4 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 |
Cytoplasm | 1 | 5 | 5 | 8 | 13 | 10 | 35 | 18 | 17 | 17 | 17 | 9 | 4 | 4 | 6 | 2 | 0 | 1 | 0 | 2 | 0 | 1 | 4 | 1 |
Endoplasmic Reticulum | 2 | 0 | 2 | 1 | 2 | 4 | 1 | 3 | 1 | 5 | 4 | 1 | 5 | 4 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 |
Endosome | 3 | 0 | 0 | 0 | 4 | 0 | 1 | 4 | 3 | 8 | 0 | 2 | 2 | 3 | 9 | 0 | 3 | 1 | 3 | 1 | 2 | 2 | 9 | 12 |
Golgi | 20 | 11 | 0 | 4 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 41 | 60 | 148 | 19 | 1 | 6 | 7 | 13 | 5 | 14 | 31 | 42 |
Mitochondria | 173 | 167 | 171 | 257 | 372 | 430 | 506 | 640 | 505 | 641 | 540 | 688 | 116 | 198 | 289 | 2 | 4 | 1 | 176 | 154 | 152 | 132 | 283 | 217 |
Nucleus | 0 | 0 | 0 | 0 | 2 | 7 | 3 | 2 | 0 | 3 | 1 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 14 | 4 | 21 | 11 | 34 | 48 | 84 | 75 | 62 | 88 | 79 | 68 | 3 | 6 | 21 | 1 | 2 | 0 | 1 | 0 | 0 | 1 | 5 | 3 |
Nucleolus | 21 | 8 | 8 | 6 | 46 | 50 | 76 | 97 | 57 | 76 | 43 | 64 | 7 | 3 | 8 | 4 | 3 | 3 | 1 | 1 | 0 | 0 | 2 | 1 |
Peroxisomes | 0 | 2 | 2 | 5 | 0 | 4 | 3 | 3 | 2 | 10 | 1 | 11 | 4 | 6 | 14 | 3 | 5 | 2 | 4 | 1 | 7 | 10 | 21 | 18 |
SpindlePole | 1 | 0 | 0 | 1 | 2 | 1 | 2 | 4 | 3 | 8 | 4 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 |
Vac/Vac Membrane | 26 | 26 | 7 | 6 | 22 | 22 | 15 | 28 | 17 | 17 | 11 | 10 | 19 | 28 | 67 | 5 | 2 | 2 | 3 | 7 | 1 | 6 | 21 | 21 |
Unique Cell Count | 194 | 188 | 180 | 271 | 402 | 468 | 577 | 726 | 538 | 709 | 581 | 736 | 145 | 234 | 398 | 42 | 16 | 16 | 201 | 192 | 176 | 186 | 399 | 351 |
Labelled Cell Count | 270 | 235 | 221 | 312 | 511 | 591 | 747 | 886 | 689 | 899 | 729 | 875 | 217 | 328 | 591 | 46 | 20 | 17 | 201 | 192 | 176 | 186 | 399 | 351 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 13.7 | 14.0 | 7.9 | 8.7 | 8.8 | 8.4 | 8.0 | 8.1 | 7.4 | 8.3 | 7.8 | 8.1 | 12.1 | 11.3 | 11.7 | 12.3 | 11.4 | 12.3 | 13.7 | 14.0 | 14.1 |
Std Deviation (1e-4) | 3.0 | 3.3 | 1.6 | 1.8 | 1.7 | 2.0 | 1.8 | 1.9 | 2.0 | 2.1 | 2.0 | 2.1 | 2.9 | 1.9 | 1.8 | 3.0 | 2.8 | 2.7 | 3.4 | 3.2 | 3.3 |
Intensity Change (Log2) | – | – | – | 0.15 | 0.16 | 0.1 | 0.02 | 0.04 | -0.09 | 0.08 | -0.01 | 0.05 | 0.62 | 0.52 | 0.57 | 0.64 | 0.54 | 0.64 | 0.79 | 0.83 | 0.84 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.1 | 0.3 | 0 | 1.7 | 0 | 0.3 | 0 | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7.6 | 7.3 | 9.5 | 0 | 0 | 0 |
Mitochondria | -0.1 | -1.1 | -1.4 | -2.8 | -2.7 | -0.6 | -2.0 | -1.0 | -0.8 | -4.2 | -3.4 | -6.2 | -12.7 | -8.7 | -10.4 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -3.1 | -1.2 | -0.5 | 1.0 | -0.5 | -0.1 | 0.3 | 0.7 | -1.0 | -3.3 | -3.7 | -2.7 | 0 | 0 | 0 |
Nucleolus | -1.3 | 2.7 | 2.5 | 3.3 | 3.3 | 2.5 | 2.6 | 1.4 | 1.9 | 0.2 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.0 | 0.8 | 0.4 | -0.9 | 0 | -0.5 | 0 | 0 | 0 | 3.0 | 2.9 | 4.3 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.6551 | 11.5743 | 11.0022 | 8.6501 | 9.4919 | 10.33 | 9.7863 | 12.2907 | 11.8076 | 9.2966 | 11.4472 | 11.377 | 11.1503 | 11.5632 | 10.934 | 9.4796 | 6.961 | 10.7341 |
Actin | 0.0206 | 0.0022 | 0.0094 | 0.0111 | 0.0055 | 0.0064 | 0.0775 | 0.0116 | 0.0306 | 0.0072 | 0 | 0.0014 | 0.0147 | 0.0019 | 0.0042 | 0.0034 | 0.0273 | 0.0003 |
Bud | 0.0005 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0002 | 0.0004 | 0.0013 | 0 |
Bud Neck | 0.001 | 0.0001 | 0.0015 | 0.0003 | 0.0001 | 0.0002 | 0.0005 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0016 | 0 | 0.0001 | 0.0123 | 0.0012 | 0.0001 |
Bud Periphery | 0.0007 | 0.0004 | 0.0006 | 0.0006 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.0004 | 0.0009 | 0.0029 | 0 |
Bud Site | 0.0045 | 0.0012 | 0.0043 | 0.0006 | 0.0002 | 0.0002 | 0.0012 | 0.0005 | 0.0006 | 0.0004 | 0 | 0 | 0.0019 | 0.0001 | 0.0003 | 0.0029 | 0.0096 | 0 |
Cell Periphery | 0.0003 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0007 | 0.0002 | 0.0002 | 0.0001 | 0.0007 | 0 |
Cytoplasm | 0.0009 | 0.0001 | 0.0002 | 0.0013 | 0.0001 | 0.0066 | 0.048 | 0 | 0 | 0.0001 | 0 | 0 | 0.0017 | 0 | 0 | 0.0004 | 0.0019 | 0.0001 |
Cytoplasmic Foci | 0.0194 | 0.0024 | 0.0044 | 0.0134 | 0.0262 | 0.0103 | 0.0033 | 0.0003 | 0.0008 | 0.0108 | 0 | 0.0002 | 0.0151 | 0.0005 | 0.001 | 0.0089 | 0.0282 | 0 |
Eisosomes | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0005 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.001 | 0.0001 |
Endoplasmic Reticulum | 0.0008 | 0.0001 | 0.0001 | 0.0026 | 0.0001 | 0.0002 | 0.0024 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0017 | 0 | 0.0001 | 0.0004 | 0.0022 | 0.0006 |
Endosome | 0.0207 | 0.0124 | 0.0065 | 0.0372 | 0.0033 | 0.0058 | 0.0062 | 0.0047 | 0.0012 | 0.0085 | 0 | 0.0008 | 0.006 | 0.0014 | 0.0015 | 0.0131 | 0.0318 | 0.0022 |
Golgi | 0.015 | 0.014 | 0.008 | 0.0252 | 0.0022 | 0.011 | 0.0106 | 0.0036 | 0.0039 | 0.0256 | 0.0001 | 0.0036 | 0.0044 | 0.0028 | 0.0027 | 0.0044 | 0.0118 | 0.0017 |
Lipid Particles | 0.0124 | 0.0043 | 0.0104 | 0.0414 | 0.0462 | 0.0123 | 0.026 | 0.0026 | 0.0103 | 0.0125 | 0.0001 | 0.0011 | 0.0205 | 0.0107 | 0.004 | 0.0065 | 0.0574 | 0.001 |
Mitochondria | 0.8629 | 0.9406 | 0.9338 | 0.8485 | 0.8791 | 0.9379 | 0.7823 | 0.9614 | 0.9354 | 0.9128 | 0.9992 | 0.989 | 0.907 | 0.959 | 0.9539 | 0.9232 | 0.7669 | 0.9772 |
None | 0.0016 | 0 | 0.0003 | 0.0009 | 0.0003 | 0.0014 | 0.0027 | 0 | 0 | 0.0001 | 0 | 0 | 0.0017 | 0 | 0.0001 | 0.0003 | 0.007 | 0.0001 |
Nuclear Periphery | 0.0006 | 0.0001 | 0.0003 | 0.001 | 0.0003 | 0.0002 | 0.0065 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0.0015 | 0.0001 | 0.0002 | 0.0046 | 0.0105 | 0.0105 |
Nucleolus | 0.0012 | 0.0003 | 0.0002 | 0.0003 | 0.0006 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0005 | 0.0003 | 0.0001 | 0.0011 | 0.0021 | 0.0001 |
Nucleus | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0046 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0007 | 0.0001 | 0.0001 | 0.0016 | 0.0023 | 0.0016 |
Peroxisomes | 0.0122 | 0.0035 | 0.0023 | 0.0058 | 0.014 | 0.0022 | 0.0222 | 0.0036 | 0.0088 | 0.0201 | 0.0002 | 0.0025 | 0.0075 | 0.0118 | 0.0066 | 0.0092 | 0.0109 | 0.0003 |
Punctate Nuclear | 0.0013 | 0 | 0.0005 | 0.0007 | 0.0057 | 0.0004 | 0.0004 | 0 | 0 | 0.0002 | 0 | 0 | 0.0041 | 0 | 0.0002 | 0.0035 | 0.0153 | 0.0001 |
Vacuole | 0.0082 | 0.0066 | 0.0033 | 0.0036 | 0.0048 | 0.0008 | 0.0011 | 0.0029 | 0.0009 | 0.0003 | 0 | 0.0002 | 0.0023 | 0.0018 | 0.0034 | 0.0015 | 0.0028 | 0.0006 |
Vacuole Periphery | 0.0144 | 0.0107 | 0.013 | 0.0047 | 0.0104 | 0.0031 | 0.0031 | 0.0073 | 0.0061 | 0.0008 | 0.0002 | 0.001 | 0.0052 | 0.0092 | 0.0206 | 0.0012 | 0.0047 | 0.0034 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 18.609 | 13.6997 | 15.2486 | 29.9459 | 21.2889 | 33.9153 | 29.6823 | 31.7197 | 25.6205 | 33.5796 |
Translational Efficiency | 1.5864 | 1.948 | 1.6759 | 1.1928 | 1.6764 | 1.0492 | 1.2114 | 1.1354 | 1.7709 | 1.4609 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1859 | 2375 | 204 | 1430 | 1794 | 1445 | 1802 | 1646 | 3653 | 3820 | 2006 | 3076 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1029.44 | 1315.56 | 1913.91 | 1571.44 | 1162.44 | 1117.02 | 1449.88 | 1521.87 | 1094.76 | 1240.46 | 1497.07 | 1544.91 |
Standard Deviation | 261.79 | 272.95 | 303.80 | 282.68 | 216.11 | 239.32 | 253.44 | 274.69 | 249.46 | 277.95 | 294.55 | 279.53 |
Intensity Change Log 2 | — | 0.353817 | 0.894663 | 0.610227 | — | -0.057501 | 0.318777 | 0.388689 | — | 0.150318 | 0.617919 | 0.496983 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000292 | 0.001147 | 0.007496 | 0.006504 | 0.000533 | 0.003800 | 0.001747 | 0.004375 | 0.000410 | 0.002150 | 0.002332 | 0.005364 |
Bud Neck | 0.000378 | 0.000329 | 0.009954 | 0.001322 | 0.000543 | 0.001048 | 0.000633 | 0.000864 | 0.000459 | 0.000601 | 0.001581 | 0.001077 |
Bud Site | 0.003881 | 0.000400 | 0.002777 | 0.002419 | 0.000832 | 0.001008 | 0.000715 | 0.002772 | 0.002384 | 0.000630 | 0.000924 | 0.002608 |
Cell Periphery | 0.001431 | 0.000080 | 0.000161 | 0.000333 | 0.000093 | 0.000360 | 0.000056 | 0.000230 | 0.000774 | 0.000186 | 0.000067 | 0.000278 |
Cytoplasm | 0.000873 | 0.000080 | 0.000317 | 0.000293 | 0.000092 | 0.000266 | 0.000218 | 0.000358 | 0.000489 | 0.000150 | 0.000228 | 0.000328 |
Cytoplasmic Foci | 0.001783 | 0.005251 | 0.055592 | 0.005780 | 0.007680 | 0.012347 | 0.006994 | 0.006209 | 0.004679 | 0.007935 | 0.011936 | 0.006009 |
Eisosomes | 0.000087 | 0.000201 | 0.000656 | 0.000440 | 0.000165 | 0.000370 | 0.000194 | 0.000241 | 0.000125 | 0.000265 | 0.000241 | 0.000334 |
Endoplasmic Reticulum | 0.000110 | 0.000076 | 0.000381 | 0.000412 | 0.000115 | 0.000198 | 0.000049 | 0.000461 | 0.000112 | 0.000122 | 0.000083 | 0.000438 |
Endosome | 0.001867 | 0.001899 | 0.007255 | 0.003170 | 0.002230 | 0.006521 | 0.001849 | 0.004112 | 0.002045 | 0.003647 | 0.002399 | 0.003674 |
Golgi | 0.002926 | 0.012392 | 0.042048 | 0.028621 | 0.009470 | 0.027081 | 0.010786 | 0.025514 | 0.006140 | 0.017948 | 0.013965 | 0.026958 |
Lipid Particles | 0.000882 | 0.002210 | 0.015310 | 0.001824 | 0.003945 | 0.007739 | 0.001050 | 0.001114 | 0.002386 | 0.004302 | 0.002500 | 0.001444 |
Mitochondria | 0.974978* | 0.964196* | 0.743089* | 0.920937* | 0.958025* | 0.916884* | 0.956896* | 0.936655* | 0.966652* | 0.946299* | 0.935153* | 0.929348* |
Mitotic Spindle | 0.000498 | 0.000148 | 0.011838 | 0.002004 | 0.000182 | 0.000743 | 0.000770 | 0.001086 | 0.000343 | 0.000373 | 0.001896 | 0.001513 |
None | 0.001486 | 0.001017 | 0.004184 | 0.001144 | 0.001109 | 0.001196 | 0.001165 | 0.000854 | 0.001301 | 0.001085 | 0.001472 | 0.000989 |
Nuclear Periphery | 0.000075 | 0.000087 | 0.008901 | 0.000281 | 0.000218 | 0.000138 | 0.000144 | 0.000161 | 0.000145 | 0.000106 | 0.001035 | 0.000217 |
Nuclear Periphery Foci | 0.000072 | 0.000096 | 0.002937 | 0.000244 | 0.000185 | 0.000215 | 0.000082 | 0.000092 | 0.000128 | 0.000141 | 0.000373 | 0.000163 |
Nucleolus | 0.000064 | 0.000507 | 0.003722 | 0.000305 | 0.000321 | 0.000649 | 0.000172 | 0.000095 | 0.000190 | 0.000561 | 0.000533 | 0.000193 |
Nucleus | 0.000173 | 0.000080 | 0.000310 | 0.000106 | 0.000054 | 0.000447 | 0.000024 | 0.000028 | 0.000114 | 0.000219 | 0.000053 | 0.000064 |
Peroxisomes | 0.001382 | 0.003202 | 0.016504 | 0.006522 | 0.005331 | 0.011671 | 0.009027 | 0.006317 | 0.003321 | 0.006406 | 0.009787 | 0.006412 |
Vacuole | 0.002694 | 0.003182 | 0.030803 | 0.005495 | 0.003185 | 0.003795 | 0.002496 | 0.003046 | 0.002935 | 0.003414 | 0.005374 | 0.004185 |
Vacuole Periphery | 0.004069 | 0.003420 | 0.035767 | 0.011843 | 0.005694 | 0.003524 | 0.004934 | 0.005417 | 0.004867 | 0.003460 | 0.008069 | 0.008404 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.20 | -3.52 | -6.66 | -5.64 | 0.43 | -5.98 | -3.29 | -4.95 | -0.62 | -3.12 | -7.10 | -5.01 | -8.26 | -5.02 | -4.31 |
Bud Neck | 0.40 | -5.11 | -3.96 | -4.55 | 4.58 | -1.70 | -0.34 | -1.06 | 0.72 | -1.14 | -0.93 | -4.41 | -3.22 | -3.06 | 1.93 |
Bud Site | 3.15 | 0.91 | 1.18 | -3.74 | 0.48 | -0.33 | 0.21 | -2.32 | -2.43 | -2.82 | 2.83 | 2.24 | -0.34 | -4.32 | -3.40 |
Cell Periphery | 2.67 | 2.51 | 2.16 | -4.02 | -2.71 | -1.75 | 1.15 | -3.40 | 0.85 | -6.65 | 2.22 | 2.74 | 1.91 | -1.41 | -6.63 |
Cytoplasm | 1.54 | 1.08 | 1.12 | -6.21 | 0.41 | -2.45 | -2.95 | -3.89 | -0.98 | -1.83 | 1.29 | 0.99 | 0.60 | -3.78 | -1.93 |
Cytoplasmic Foci | -5.36 | -6.78 | -6.06 | -0.68 | 6.27 | -3.43 | 0.57 | 1.30 | 4.70 | 0.70 | -4.66 | -5.90 | -2.05 | 2.67 | 4.77 |
Eisosomes | -7.11 | -5.04 | -6.06 | -4.10 | 1.71 | -3.54 | -1.50 | -3.49 | 2.20 | -2.15 | -5.76 | -6.01 | -7.06 | -1.90 | -2.81 |
Endoplasmic Reticulum | 1.28 | -3.20 | -3.44 | -3.94 | -0.27 | -2.19 | 2.51 | -1.70 | -1.29 | -2.04 | -0.46 | 1.46 | -2.80 | -2.72 | -3.07 |
Endosome | -0.07 | -3.30 | -2.33 | -2.67 | 2.49 | -5.02 | 0.78 | -2.78 | 2.46 | -3.39 | -3.74 | -0.82 | -3.64 | -0.08 | -2.57 |
Golgi | -7.20 | -5.81 | -9.42 | -5.51 | 1.85 | -6.54 | -0.82 | -6.17 | 0.45 | -5.51 | -8.81 | -5.50 | -11.12 | -4.18 | -5.89 |
Lipid Particles | -3.52 | -4.58 | -2.11 | 0.72 | 4.25 | -2.52 | 3.72 | 3.51 | 5.00 | -0.23 | -2.90 | -0.22 | 2.06 | 4.89 | 2.46 |
Mitochondria | 3.07 | 10.78 | 9.85 | 7.96 | -8.12 | 6.75 | 0.25 | 4.09 | -2.95 | 3.87 | 6.31 | 7.20 | 9.90 | 4.22 | 1.17 |
Mitotic Spindle | 0.94 | -2.99 | -2.85 | -4.86 | 2.59 | -2.95 | -4.94 | -5.01 | -1.34 | -1.54 | -0.16 | -3.49 | -4.26 | -5.34 | 0.84 |
None | 1.22 | -4.78 | 0.88 | -1.74 | 7.29 | -0.26 | -0.61 | 3.73 | 1.01 | 3.72 | 0.91 | -0.80 | 1.56 | 0.72 | 5.51 |
Nuclear Periphery | -0.90 | -2.49 | -3.98 | -3.77 | 2.43 | 1.52 | 1.43 | 0.94 | -0.60 | -0.45 | 1.48 | -2.43 | -1.86 | -3.55 | 2.23 |
Nuclear Periphery Foci | -0.85 | -3.81 | -1.89 | -1.64 | 3.55 | -0.60 | 2.99 | 2.54 | 3.15 | -0.61 | -0.50 | -3.01 | -0.78 | -0.51 | 2.31 |
Nucleolus | -2.17 | -2.83 | -1.71 | 0.79 | 2.63 | -0.98 | 0.83 | 1.27 | 1.95 | 1.82 | -1.98 | -2.11 | -0.05 | 2.04 | 2.21 |
Nucleus | 0.78 | -0.85 | 0.48 | -0.38 | 1.50 | -1.66 | 1.55 | 1.29 | 1.77 | -0.68 | -0.96 | 1.01 | 0.72 | 1.58 | -0.35 |
Peroxisomes | -2.81 | -3.71 | -4.38 | -2.64 | 2.37 | -3.61 | -2.29 | -0.76 | 2.90 | 1.58 | -3.79 | -4.68 | -3.54 | -0.01 | 2.24 |
Vacuole | -0.69 | -4.71 | -3.16 | -2.68 | 4.22 | -0.74 | 1.15 | 0.16 | 0.83 | -0.89 | -0.91 | -3.06 | -2.26 | -1.34 | 1.38 |
Vacuole Periphery | 1.05 | -6.92 | -5.69 | -6.35 | 5.05 | 2.32 | 0.72 | 0.27 | -2.50 | -0.55 | 2.54 | -3.46 | -4.33 | -6.74 | -0.33 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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