Standard name
Human Ortholog
Description (SGD) Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud 0 0 0 0.05 0.06 0 0.08 0.05 0 0.06 0.06 0 0 0 0 – 0.05 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.1 – 0 0 0 0 0 0
Cytoplasm 0.33 0.24 0.21 0.27 0.11 0.08 0.05 0 0.05 0 0.05 0 0.7 0.67 0.69 – 0.1 – 0.05 0 0 0 0 0.06
Endoplasmic Reticulum 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0.05 0 0 – 0.05 – 0 0 0 0 0 0
Endosome 0.17 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 – 0.3 – 0.08 0.07 0.07 0.05 0.06 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.05 – 0 0 0 0 0 0
Mitochondria 0.5 0.62 0.85 0.78 0.91 0.94 0.94 0.94 0.94 0.95 0.95 0.97 0.14 0.12 0.07 – 0.05 – 0.69 0.69 0.73 0.7 0.67 0.63
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.05 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 – 0.05 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Peroxisomes 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 – 0.15 – 0 0 0 0 0 0
SpindlePole 0.06 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.15 0 0 0.05 0.05 0 0 0.05 0.05 0.06 0 0.06 0.09 0.11 – 0.15 – 0 0.07 0 0.06 0.1 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 4 0 0 0 0 0 0 0 0 0 0 5 5 3 – 0 – 2 10 2 5 7 13
Bud 2 5 2 12 24 16 45 47 21 34 29 28 6 8 2 – 1 – 2 3 0 2 5 4
Bud Neck 1 0 0 1 0 1 0 0 1 0 1 0 4 6 3 – 0 – 1 4 0 3 6 6
Bud Site 1 2 0 3 0 9 10 10 4 7 11 9 0 1 0 – 0 – – – – – – –
Cell Periphery 4 4 0 2 3 3 0 2 2 3 2 8 4 5 10 – 2 – 0 1 0 0 1 1
Cytoplasm 67 67 15 65 43 34 32 39 32 20 24 14 148 209 257 – 2 – 13 9 2 6 10 22
Endoplasmic Reticulum 0 15 4 0 6 3 2 2 0 1 3 10 10 3 12 – 1 – 3 5 2 0 3 5
Endosome 34 15 0 2 2 0 0 2 0 0 0 0 5 17 26 – 6 – 20 27 6 10 18 16
Golgi 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 – 1 – 4 7 0 3 3 6
Mitochondria 104 174 60 187 363 405 547 816 565 574 500 673 29 36 26 – 1 – 184 258 69 136 214 234
Nucleus 1 0 0 1 3 15 14 26 7 10 7 5 1 0 1 – 1 – 0 1 0 0 0 0
Nuclear Periphery 0 0 1 0 3 11 8 23 25 31 21 21 0 1 0 – 1 – 0 0 1 0 1 1
Nucleolus 1 0 0 1 2 8 16 21 18 14 10 23 0 0 0 – 0 – 1 0 0 0 1 0
Peroxisomes 10 6 0 2 10 1 3 3 2 2 4 1 0 1 31 – 3 – 7 3 3 3 2 8
SpindlePole 13 2 0 14 12 15 20 34 11 27 17 19 4 12 9 – 0 – 6 4 0 5 3 5
Vac/Vac Membrane 23 42 1 3 19 22 26 30 29 28 30 19 13 28 40 – 3 – 10 27 3 11 31 42
Unique Cell Count 206 280 71 240 401 433 585 869 602 607 524 694 211 312 371 20 269 374 95 194 318 375
Labelled Cell Count 262 339 83 293 490 543 723 1055 717 751 659 830 229 335 420 22 269 374 95 194 318 375


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 4.6 3.4 3.4 3.7 3.5 3.3 3.5 3.4 3.4 3.5 3.4 5.0 5.0 5.1 – 7.4 – 5.6 5.5 5.7
Std Deviation (1e-4) 1.1 1.0 0.9 1.1 1.2 1.1 1.4 1.1 1.1 1.1 1.4 0.9 1.0 1.0 0.9 – 2.2 – 1.6 1.3 1.5
Intensity Change (Log2) – – – -0.01 0.12 0.06 -0.04 0.06 0.01 0.02 0.06 0.02 0.57 0.55 0.59 – 1.13 – 0.72 0.7 0.74


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.3518 0.1624 0.5931 0.3574 -0.0462 0.2716 0.3995 1.3012 1.0647 0.9664 1.0227 0.8639 -1.676 -1.0762 -1.5383 -1.1143 -1.0357 -1.1751
Actin 0.0315 0.0217 0.0368 0.0142 0.0089 0.0165 0.0395 0.0113 0.0414 0.0025 0.0224 0.0158 0.0167 0.0088 0.0045 0.01 0.0099 0.012
Bud 0.0011 0.0034 0.0025 0.0034 0.0022 0.0011 0.0036 0.0057 0.0026 0.0111 0.0049 0.0014 0.0009 0.0016 0.0016 0.0059 0.0014 0.0016
Bud Neck 0.002 0.0024 0.0103 0.0012 0.0172 0.0145 0.0052 0.0018 0.0081 0.0014 0.0022 0.0138 0.0016 0.0013 0.0029 0.0077 0.0018 0.0334
Bud Periphery 0.0031 0.0089 0.0072 0.0232 0.0071 0.0042 0.014 0.0126 0.0095 0.0743 0.0136 0.004 0.0021 0.0034 0.0054 0.0147 0.0022 0.0041
Bud Site 0.0045 0.0387 0.015 0.0011 0.0044 0.0016 0.0186 0.0506 0.0232 0.0031 0.0088 0.0018 0.0048 0.019 0.0104 0.0104 0.0023 0.0023
Cell Periphery 0.0019 0.0023 0.0023 0.0021 0.0069 0.0029 0.0027 0.0036 0.0061 0.0017 0.0016 0.0015 0.002 0.002 0.0014 0.0016 0.0005 0.0015
Cytoplasm 0.0503 0.0808 0.0527 0.072 0.0649 0.0742 0.0626 0.1214 0.0748 0.1244 0.0953 0.1265 0.0716 0.1984 0.107 0.0927 0.0901 0.1326
Cytoplasmic Foci 0.0944 0.1261 0.1015 0.1112 0.2126 0.1346 0.1137 0.0537 0.0832 0.0451 0.1137 0.1582 0.1106 0.1432 0.1007 0.1353 0.1688 0.1424
Eisosomes 0.0012 0.001 0.0016 0.0002 0.0011 0.0017 0.0015 0.0012 0.0034 0.0006 0.0006 0.0005 0.0016 0.0014 0.0009 0.0009 0.0016 0.0007
Endoplasmic Reticulum 0.008 0.0037 0.0023 0.0044 0.0043 0.004 0.0071 0.0044 0.0038 0.0039 0.0035 0.0029 0.0096 0.0089 0.0049 0.008 0.0047 0.0041
Endosome 0.1441 0.1236 0.1026 0.0809 0.1017 0.1191 0.0822 0.0519 0.0649 0.0369 0.1233 0.0855 0.1378 0.1357 0.0872 0.0924 0.2182 0.1197
Golgi 0.0446 0.0528 0.0394 0.0377 0.0355 0.0396 0.0215 0.0225 0.0201 0.012 0.0563 0.0242 0.0361 0.0351 0.0313 0.0272 0.0693 0.0419
Lipid Particles 0.0548 0.033 0.0163 0.0436 0.0171 0.0194 0.0338 0.0041 0.0113 0.0341 0.0333 0.011 0.0473 0.0288 0.0185 0.0359 0.033 0.0317
Mitochondria 0.2966 0.2513 0.3463 0.3866 0.216 0.2998 0.161 0.1643 0.206 0.3238 0.1636 0.2316 0.2623 0.096 0.2482 0.2584 0.2318 0.2851
None 0.1731 0.1802 0.2039 0.1563 0.1614 0.2007 0.3554 0.4501 0.4063 0.239 0.263 0.2563 0.2428 0.2726 0.3202 0.2088 0.0599 0.1148
Nuclear Periphery 0.0104 0.0023 0.0022 0.0117 0.0052 0.0027 0.0066 0.0009 0.0016 0.0007 0.0007 0.0008 0.0038 0.0018 0.0021 0.011 0.0196 0.0006
Nucleolus 0.0013 0.0013 0.002 0.001 0.0028 0.0007 0.0018 0.0032 0.0026 0.0011 0.0008 0.0011 0.0012 0.001 0.0012 0.0029 0.0048 0.0011
Nucleus 0.0035 0.0015 0.0023 0.0028 0.0059 0.0017 0.0022 0.0054 0.0018 0.0018 0.0011 0.0014 0.0015 0.0014 0.0019 0.0066 0.006 0.0014
Peroxisomes 0.0502 0.0447 0.0411 0.0251 0.0986 0.0461 0.0405 0.0132 0.0155 0.0681 0.0728 0.0468 0.0215 0.0215 0.0241 0.038 0.045 0.0508
Punctate Nuclear 0.0032 0.0026 0.0024 0.0066 0.0128 0.0025 0.007 0.0009 0.0029 0.0007 0.0008 0.0022 0.0024 0.0015 0.0013 0.0115 0.0157 0.0015
Vacuole 0.0144 0.0127 0.0065 0.0098 0.0106 0.0083 0.016 0.0149 0.0081 0.01 0.0118 0.0092 0.0164 0.0133 0.0182 0.013 0.0087 0.0115
Vacuole Periphery 0.0059 0.0051 0.0029 0.0047 0.0028 0.0043 0.0033 0.0024 0.0029 0.0039 0.0059 0.0033 0.0054 0.0031 0.0061 0.0069 0.0048 0.005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.5281 11.5366 8.5606 13.8165 9.7605 8.5988 10.2065 14.4335 24.3148 12.5787
Translational Efficiency 2.4099 1.0666 1.6771 1.3628 1.5067 2.1514 1.5852 1.3346 0.714 1.0605

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘


Micrographs

Cell Count

Number of cells analysed for each strain. R1, R2: biological replicates; R1 & R2: both replicates combined.

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
914 1896 903 N/A 964 2294 450 1937 1878 4190 1353 1937

Protein Abundance

Mean and standard deviation of cell GFP intensity, and intensity change (log2) of each mutant relative to wild type.

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 584.06 760.95 875.46 N/A 561.23 722.50 892.91 871.39 572.34 739.90 881.26 871.39
Standard Deviation 75.05 99.32 125.26 N/A 71.40 264.43 88.39 113.85 74.08 207.63 114.62 113.85
Intensity Change Log 2 — 0.381685 0.583925 N/A — 0.364405 0.669923 0.634726 — 0.373243 0.626707 —

Localization Score (DeepLoc)

Localization score for each compartment. Values highlighted in green denote significant localization scores (marked * in the plot).

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000394 0.001017 0.004401 N/A 0.000306 0.000530 0.005862 0.014330 0.000349 0.000750 0.004887 0.014330
Bud Neck 0.022370 0.032486 0.015653 N/A 0.049944 0.040739 0.040276 0.039794 0.036524 0.037004 0.023843 0.039794
Bud Site 0.010054 0.037922 0.063215 N/A 0.023302 0.024307 0.080118 0.147916* 0.016854 0.030468 0.068837 0.147916*
Cell Periphery 0.001304 0.000212 0.000205 N/A 0.000375 0.000274 0.000213 0.000362 0.000827 0.000246 0.000207 0.000362
Cytoplasm 0.029921 0.072025 0.107567* N/A 0.128829* 0.048520 0.159496* 0.191078* 0.080692 0.059156 0.124838* 0.191078*
Cytoplasmic Foci 0.111984 0.224199* 0.159615 N/A 0.223659* 0.155513 0.169153 0.144942 0.169308 0.186594* 0.162787 0.144942
Eisosomes 0.000258 0.000173 0.000090 N/A 0.000250 0.000143 0.000143 0.000255 0.000254 0.000156 0.000107 0.000255
Endoplasmic Reticulum 0.001626 0.001138 0.007765 N/A 0.001747 0.000709 0.004852 0.003738 0.001688 0.000904 0.006796 0.003738
Endosome 0.026230 0.086928 0.078401 N/A 0.087701 0.099909 0.061493 0.030952 0.057784 0.094035 0.072778 0.030952
Golgi 0.072062 0.084966 0.078043 N/A 0.036367 0.075506 0.019060 0.032386 0.053739 0.079787 0.058426 0.032386
Lipid Particles 0.025550 0.006399 0.005990 N/A 0.038089 0.008321 0.006641 0.002923 0.031987 0.007451 0.006207 0.002923
Mitochondria 0.619337* 0.288622* 0.087674 N/A 0.231309* 0.406388* 0.031321 0.093876 0.420158* 0.353098* 0.068932 0.093876
Mitotic Spindle 0.002845 0.023136 0.128895* N/A 0.004695 0.014815 0.081890 0.040516 0.003795 0.018580 0.113261* 0.040516
None 0.002121 0.001197 0.001767 N/A 0.006921 0.001201 0.001441 0.000430 0.004585 0.001199 0.001659 0.000430
Nuclear Periphery 0.000339 0.000240 0.002304 N/A 0.001741 0.000311 0.001290 0.000347 0.001059 0.000279 0.001967 0.000347
Nuclear Periphery Foci 0.000497 0.000278 0.003484 N/A 0.000986 0.000182 0.001883 0.000417 0.000748 0.000225 0.002951 0.000417
Nucleolus 0.002245 0.001818 0.004958 N/A 0.011123 0.001573 0.003288 0.000665 0.006802 0.001684 0.004403 0.000665
Nucleus 0.008801 0.014394 0.056886 N/A 0.058048 0.011357 0.083548 0.036900 0.034080 0.012731 0.065754 0.036900
Peroxisomes 0.039680 0.044787 0.040871 N/A 0.036260 0.029945 0.079276 0.095628 0.037924 0.036661 0.053644 0.095628
Vacuole 0.008366 0.059951 0.117595 N/A 0.037572 0.059250 0.162485 0.115708 0.023358 0.059567 0.132525 0.115708
Vacuole Periphery 0.014015 0.018113 0.034620 N/A 0.020776 0.020507 0.006272 0.006837 0.017485 0.019424 0.025191 0.006837

Localization Changes (T-score)

Change in localization of each mutant relative to its control (e.g. UBP2_WT: ubp2Δ vs wild type). Values highlighted in yellow (increase) or blue (decrease) denote significant changes (marked * in the plot).

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin N/A N/A N/A N/A N/A -2.00 -4.14 -12.07 -11.82 -4.97 -3.81 -8.45 -12.03 -11.63 -7.11
Bud Neck N/A N/A N/A N/A N/A 2.08 2.14 2.57 0.55 -0.22 -0.34 4.73 -1.11 -0.98 -6.72
Bud Site N/A N/A N/A N/A N/A -0.59 -7.96 -24.09 -24.97 -8.40 -7.97 -14.70 -26.90 -23.92 -13.22
Cell Periphery N/A N/A N/A N/A N/A 3.01 4.91 0.87 -1.82 -3.65 2.77 2.96 2.32 -2.98 -4.27
Cytoplasm N/A N/A N/A N/A N/A 12.11 -3.07 -8.70 -26.68 -3.02 5.17 -7.36 -18.86 -25.57 -9.38
Cytoplasmic Foci N/A N/A N/A N/A N/A 9.58 6.66 11.85 3.06 2.53 -3.13 2.11 5.44 10.24 3.18
Eisosomes N/A N/A N/A N/A N/A 7.48 6.34 0.27 -7.62 -6.36 7.28 11.08 0.51 -6.56 -10.27
Endoplasmic Reticulum N/A N/A N/A N/A N/A 3.19 -6.30 -3.27 -5.59 2.25 3.27 -9.62 -3.56 -5.20 4.62
Endosome N/A N/A N/A N/A N/A -2.44 4.90 12.86 21.79 6.04 -11.38 -3.24 10.07 26.22 12.94
Golgi N/A N/A N/A N/A N/A -11.25 5.66 2.38 15.00 -4.13 -8.03 -0.29 7.96 19.21 6.97
Lipid Particles N/A N/A N/A N/A N/A 10.17 10.48 12.24 7.63 4.55 12.28 12.50 14.70 9.03 5.52
Mitochondria N/A N/A N/A N/A N/A -15.09 19.21 13.73 40.00* -10.05 6.66 36.89 34.94* 41.91* -3.54
Mitotic Spindle N/A N/A N/A N/A N/A -7.02 -8.55 -11.98 -8.25 4.09 -12.36 -19.56 -12.57 -7.12 11.40
None N/A N/A N/A N/A N/A 5.20 4.08 5.91 4.99 2.04 5.51 4.29 6.77 6.84 4.80
Nuclear Periphery N/A N/A N/A N/A N/A 2.71 0.55 2.49 -1.70 4.86 2.83 -2.81 2.29 -2.69 6.79
Nuclear Periphery Foci N/A N/A N/A N/A N/A 5.59 -3.62 3.74 -4.49 6.17 4.69 -8.21 2.64 -3.62 10.02
Nucleolus N/A N/A N/A N/A N/A 5.93 4.81 6.51 4.01 5.83 5.66 2.49 6.76 5.55 6.66
Nucleus N/A N/A N/A N/A N/A 10.66 -4.40 3.05 -12.65 6.80 8.28 -8.07 -2.84 -12.65 6.21
Peroxisomes N/A N/A N/A N/A N/A 2.22 -5.03 -11.84 -15.02 -2.39 0.55 -3.48 -12.25 -13.57 -7.94
Vacuole N/A N/A N/A N/A N/A -6.71 -14.83 -20.92 -15.83 4.42 -17.05 -24.58 -25.70 -16.69 1.04
Vacuole Periphery N/A N/A N/A N/A N/A -0.04 7.92 7.72 13.37 -0.76 -1.62 -3.41 8.81 15.33 10.43
Standard name
Human Ortholog
Description (SGD) Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex
Localization
Cell Percentages mitochondrion (62%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Cbp4

Cbp4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cbp4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available