Standard name
Human Ortholog
Description ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites; human homolog TRNT1 complements yeast null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.97 0.88 0.81 0.79 0.65 0.6 0.63 0.57 0.54 0.51 1.0 0.99 0.99 0.96 0.96 0.95 0.94 0.86 0.88 0.94 0.92 0.88
Endoplasmic Reticulum 0 0 0 0 0 0.06 0 0 0.06 0.05 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0.08 0.15 0.26 0.22 0.35 0.41 0.46 0.48 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.06 0.11 0 0.15 0.25 0.07 0.09 0.11 0.08 0 0 0 0 0 0.05 0 0.07 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 0 1 2 1 1 5 3 0 2 0 0 0 0 1 0 0 0 0 4 0 1 3
Bud 1 0 3 1 3 2 3 8 3 6 3 8 0 0 0 0 0 0 0 0 2 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2
Bud Site 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 10 13 6 8 8 12 11 9 11 7 5 6 2 5 5 6 8 0 0 1 0 0 1
Cytoplasm 284 251 448 232 260 393 355 381 259 256 117 132 419 334 457 169 138 101 271 229 433 230 330 341
Endoplasmic Reticulum 0 4 4 4 4 29 4 8 23 22 8 6 1 1 2 8 4 6 1 2 17 1 2 7
Endosome 0 0 0 3 5 6 24 20 4 5 7 4 0 1 0 0 0 1 2 1 8 0 5 3
Golgi 1 0 0 1 1 1 1 0 1 0 0 0 0 1 1 1 0 1 1 2 1 0 0 2
Mitochondria 2 0 6 13 27 76 143 141 143 185 99 125 0 0 2 2 1 1 0 1 2 3 2 3
Nucleus 2 10 0 3 2 1 5 6 8 4 1 5 2 1 0 4 2 0 2 3 1 0 2 1
Nuclear Periphery 0 0 0 4 0 1 2 2 3 8 1 2 0 0 0 0 0 0 1 2 3 0 2 0
Nucleolus 0 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 1 0 1 0 1 2
Vac/Vac Membrane 4 0 6 17 37 22 80 159 30 41 23 21 0 0 2 5 6 5 3 18 4 4 4 8
Unique Cell Count 289 259 460 264 322 497 543 640 414 450 215 259 421 337 461 176 144 106 289 266 494 246 359 388
Labelled Cell Count 299 276 480 285 349 540 631 743 487 539 269 309 428 340 469 195 157 124 289 266 494 246 359 388


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.4 13.0 11.1 10.4 9.7 8.6 7.6 7.5 7.3 6.6 6.6 6.3 12.4 12.2 11.8 12.5 16.9 16.7 11.0 10.9 12.9
Std Deviation (1e-4) 1.5 2.5 1.7 1.7 1.6 1.5 1.4 1.5 1.4 1.2 1.3 1.2 1.9 1.6 1.6 3.4 4.2 4.6 2.0 1.8 2.4
Intensity Change (Log2) – – – -0.1 -0.19 -0.36 -0.55 -0.56 -0.61 -0.75 -0.74 -0.83 0.16 0.14 0.09 0.17 0.61 0.59 -0.02 -0.02 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.4 -0.3 -1.3 -0.6 -1.2 -0.6 -0.4 0.3 -0.7 -1.4 -2.3 -1.9 0 0 0
Cytoplasm -5.2 -7.8 -8.7 -12.6 -14.4 -13.1 -14.6 -14.1 -15.1 2.5 1.8 2.0 -0.9 -1.0 -1.1
Endoplasmic Reticulum 0 0 4.2 0 0.6 4.0 3.6 0 0 0 0 0 0 0 0
Endosome 0 0 0 4.6 3.8 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.9 4.8 7.7 11.1 10.0 13.0 14.7 14.9 15.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.8 6.1 2.9 7.6 10.8 4.4 5.3 5.6 4.6 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.2312 8.6481 8.4827 8.9113 7.7402 8.6217 8.2799 10.7466 8.678 8.9225 9.0628 9.3322 10.1571 12.3469 10.6852 10.6903 10.6766 11.3102
Actin 0.2079 0.002 0.0011 0.0047 0.0008 0.0007 0.0711 0.0004 0.052 0.0036 0.0005 0.0017 0.0204 0.0003 0.0194 0.002 0.0341 0.0015
Bud 0.0019 0.0002 0.0004 0.0004 0.0006 0.0001 0.0005 0.0001 0.0026 0.0166 0.0001 0.0001 0.0016 0.0001 0.0032 0.0003 0.0001 0.0001
Bud Neck 0.0014 0.0003 0.0006 0.0011 0.0016 0.001 0.0022 0.0001 0.0008 0.0004 0.0005 0.0011 0.0018 0.0001 0.0017 0.0012 0.0004 0.0015
Bud Periphery 0.0025 0.0001 0.0001 0.0001 0.0003 0 0.0005 0 0.0019 0.0474 0 0 0.0013 0 0.0014 0.0007 0 0
Bud Site 0.0064 0.001 0.0006 0.0004 0.0002 0.0001 0.0011 0.0004 0.0128 0.0005 0.0001 0.0001 0.0014 0.0003 0.0053 0.0003 0.0001 0.0001
Cell Periphery 0.001 0.0005 0.0001 0.0001 0.0003 0 0.0001 0 0.0002 0.0001 0 0 0.0002 0 0.0004 0.001 0.0001 0
Cytoplasm 0.028 0.982 0.9344 0.9369 0.9787 0.95 0.7956 0.9679 0.8354 0.9181 0.9867 0.9611 0.858 0.9896 0.9016 0.8906 0.9123 0.9766
Cytoplasmic Foci 0.0253 0.0014 0.0085 0.0229 0.0019 0.0022 0.0222 0.0007 0.0249 0.0043 0.0046 0.0069 0.0284 0.0024 0.0185 0.0044 0.0038 0.0068
Eisosomes 0.0015 0 0 0 0 0 0.0001 0 0.0001 0 0 0 0.0002 0 0.0003 0.002 0.0012 0
Endoplasmic Reticulum 0.0508 0.0063 0.0026 0.0032 0.0055 0.0037 0.0035 0.0005 0.0035 0.001 0.002 0.0023 0.0101 0.0012 0.0028 0.0123 0.0012 0.0022
Endosome 0.0743 0.0011 0.0332 0.0156 0.0019 0.0017 0.011 0.0002 0.0244 0.0025 0.0026 0.0097 0.023 0.0008 0.0127 0.0126 0.0014 0.0042
Golgi 0.0305 0.0006 0.002 0.0041 0.0006 0.0001 0.0104 0 0.0159 0.0003 0.0003 0.0121 0.0065 0.0004 0.0064 0.0018 0.002 0.0019
Lipid Particles 0.0195 0.0001 0.0001 0.0002 0 0 0.0034 0 0.0011 0 0 0.0001 0.0038 0 0.0045 0.0015 0.0011 0
Mitochondria 0.01 0.0001 0.0012 0.0031 0.0004 0.0001 0.001 0 0.0097 0.0007 0.0002 0.0011 0.0083 0.0004 0.004 0.001 0.0003 0.0004
None 0.5109 0.0011 0.0014 0.0005 0.0007 0.0339 0.0656 0.0279 0.0049 0.0007 0.0007 0.0011 0.0069 0.003 0.0031 0.0408 0.0322 0.0013
Nuclear Periphery 0.0139 0.0009 0.0015 0.0013 0.0008 0.0013 0.0006 0.0003 0.0006 0.0008 0.0002 0.0004 0.0045 0.0002 0.0014 0.0031 0.0008 0.0006
Nucleolus 0.0003 0 0 0 0 0 0 0 0.0001 0 0 0 0.0044 0 0.0005 0.0059 0.0024 0
Nucleus 0.0018 0.0015 0.003 0.0023 0.002 0.0041 0.0007 0.0013 0.0012 0.0011 0.001 0.0013 0.0043 0.0008 0.0037 0.0083 0.0021 0.0018
Peroxisomes 0.0041 0 0.0003 0.0018 0 0 0.0092 0 0.0039 0.0001 0 0.0002 0.0071 0.0001 0.0054 0.0002 0.0014 0.0002
Punctate Nuclear 0.0031 0.0001 0.0003 0.0005 0.0001 0.0002 0.0005 0.0001 0.0004 0.0001 0.0001 0.0001 0.005 0.0001 0.0025 0.0057 0.0026 0.0002
Vacuole 0.0032 0.0007 0.0066 0.0005 0.003 0.0006 0.0006 0.0001 0.003 0.001 0.0003 0.0003 0.002 0.0001 0.0009 0.0035 0.0002 0.0003
Vacuole Periphery 0.0019 0.0001 0.0018 0.0004 0.0006 0.0002 0.0001 0 0.0007 0.0005 0.0001 0.0002 0.0007 0 0.0004 0.0006 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 32.9475 25.2455 35.2363 33.8592 31.3845 28.8971 31.6032 27.2778 28.9058 31.923
Translational Efficiency 1.3461 1.5736 1.1338 1.2489 1.0306 1.0769 1.1039 1.0011 1.1066 0.8949

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
630 1390 1473 1264 2170 2223 160 1471 2800 3613 1633 2735

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 915.99 1127.78 1288.80 1463.74 1101.94 1123.75 1476.53 1407.90 1060.10 1125.30 1307.19 1433.71
Standard Deviation 122.15 186.31 210.59 221.84 143.80 178.33 183.88 216.32 159.41 181.45 215.48 220.65
Intensity Change Log 2 — 0.300082 0.492625 0.676256 — 0.028275 0.422165 0.353499 — 0.158030 0.454576 0.508999

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000471 0.000778 0.000309 0.012071 0.000492 0.000642 0.001086 0.000842 0.000487 0.000694 0.000385 0.006031
Bud Neck 0.003137 0.003723 0.001608 0.004844 0.003734 0.004954 0.004183 0.004678 0.003599 0.004480 0.001861 0.004755
Bud Site 0.028891 0.067269 0.004235 0.132071* 0.028207 0.043654 0.026980 0.051112 0.028361 0.052739 0.006464 0.088528
Cell Periphery 0.000140 0.000099 0.000098 0.000381 0.000077 0.000056 0.000191 0.000102 0.000091 0.000073 0.000107 0.000231
Cytoplasm 0.685354* 0.646275* 0.714781* 0.640725* 0.608545* 0.655822* 0.656335* 0.637103* 0.625827* 0.652149* 0.709055* 0.638777*
Cytoplasmic Foci 0.187015* 0.169870 0.013311 0.089295 0.221098* 0.160463 0.049664 0.085976 0.213429* 0.164082 0.016873 0.087510
Eisosomes 0.000023 0.000011 0.000013 0.000020 0.000016 0.000012 0.000018 0.000008 0.000017 0.000012 0.000013 0.000013
Endoplasmic Reticulum 0.003969 0.001094 0.002619 0.000406 0.002886 0.000722 0.001151 0.000355 0.003129 0.000865 0.002475 0.000379
Endosome 0.011101 0.018554 0.003714 0.007650 0.019077 0.018605 0.011181 0.022502 0.017282 0.018585 0.004445 0.015638
Golgi 0.005756 0.017940 0.000322 0.016145 0.017128 0.014759 0.002434 0.012621 0.014569 0.015983 0.000529 0.014250
Lipid Particles 0.001543 0.001043 0.001283 0.004861 0.001733 0.000743 0.003063 0.002550 0.001690 0.000858 0.001458 0.003618
Mitochondria 0.001554 0.001341 0.000247 0.000400 0.003325 0.003426 0.000095 0.001226 0.002927 0.002624 0.000232 0.000844
Mitotic Spindle 0.001822 0.005767 0.006863 0.017840 0.004249 0.010961 0.014057 0.034913 0.003703 0.008963 0.007568 0.027023
None 0.001791 0.000677 0.004874 0.000360 0.000703 0.000816 0.001444 0.000873 0.000948 0.000763 0.004538 0.000636
Nuclear Periphery 0.000064 0.000057 0.000794 0.000079 0.000094 0.000134 0.000491 0.000222 0.000087 0.000104 0.000765 0.000156
Nuclear Periphery Foci 0.002122 0.001529 0.003707 0.001862 0.000857 0.000529 0.010554 0.001970 0.001141 0.000913 0.004378 0.001920
Nucleolus 0.000483 0.000267 0.000339 0.000122 0.000333 0.000597 0.000160 0.000354 0.000367 0.000470 0.000322 0.000247
Nucleus 0.015037 0.007831 0.135692* 0.011148 0.013182 0.010115 0.082988 0.011688 0.013599 0.009236 0.130528* 0.011438
Peroxisomes 0.001986 0.003761 0.000412 0.012741 0.001931 0.004382 0.001225 0.005107 0.001944 0.004143 0.000492 0.008635
Vacuole 0.042830 0.045184 0.104138 0.045020 0.063560 0.060780 0.132145 0.117140 0.058896 0.054780 0.106882 0.083809
Vacuole Periphery 0.004913 0.006928 0.000641 0.001960 0.008774 0.007829 0.000556 0.008659 0.007906 0.007482 0.000633 0.005563

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.26 1.60 -8.12 -7.85 -8.22 -1.00 -3.33 -2.47 -0.78 1.70 -1.99 0.80 -8.20 -7.79 -8.33
Bud Neck -1.14 1.95 -4.58 -4.14 -10.74 -3.09 -0.91 -2.79 0.58 -0.65 -2.87 4.66 -5.20 -1.81 -10.98
Bud Site -9.59 10.58 -16.57 -8.16 -23.93* -6.51 1.85 -5.01 0.88 -4.84 -11.67 17.95 -16.79 -7.11 -26.82*
Cell Periphery 2.78 3.04 -7.29 -9.21 -9.45 5.50 -7.15 -1.99 -4.62 5.73 3.78 -2.85 -8.99 -10.40 -7.39
Cytoplasm 2.86 2.94 3.33 0.50 0.68 -5.26 2.77 -2.97 1.90 -4.02 -3.57 -1.48 -1.73 1.80 -0.09
Cytoplasmic Foci 2.03 23.09 13.13 14.78 -21.31 10.19 28.15 28.03 18.16 -6.40 10.03 49.24 31.02 23.24 -26.73
Eisosomes 5.89 4.00 1.33 -6.67 -3.71 2.48 -3.62 9.09 3.17 7.83 5.05 1.57 4.08 -1.76 2.05
Endoplasmic Reticulum 2.15 1.34 2.71 3.07 7.81 5.12 4.11 6.48 2.73 5.04 5.09 2.23 6.49 4.04 8.39
Endosome -4.56 7.21 4.15 9.21 -5.79 0.56 6.98 2.75 2.15 -4.42 -1.03 16.97 6.33 7.27 -10.34
Golgi -8.82 10.88 -3.44 3.71 -7.71 2.29 16.92 7.26 5.42 -5.26 -1.09 20.74 5.28 6.22 -9.84
Lipid Particles 1.86 0.91 -7.51 -8.96 -8.37 7.63 -3.53 -4.95 -9.40 0.07 7.12 1.33 -9.00 -12.32 -9.35
Mitochondria 0.29 1.77 1.68 3.26 -1.21 -0.04 5.88 4.58 5.40 -4.42 0.64 5.91 5.17 6.37 -3.67
Mitotic Spindle -3.68 -3.48 -7.49 -5.26 -4.51 -4.44 -1.84 -8.82 -6.09 -2.00 -4.97 -2.73 -10.87 -7.57 -7.55
None 1.70 -3.22 2.15 1.18 11.70 -0.78 -3.27 -0.04 0.56 2.68 1.05 -8.91 2.11 1.37 11.11
Nuclear Periphery 0.22 -11.14 -3.56 -4.01 10.38 -0.78 -8.59 -6.88 -2.33 5.79 -0.59 -11.71 -7.26 -2.63 9.87
Nuclear Periphery Foci 1.32 -4.88 -1.33 -2.72 3.99 3.40 -6.49 -7.17 -9.38 5.53 1.40 -11.44 -7.53 -7.93 7.00
Nucleolus 0.85 0.56 1.36 3.70 8.45 -1.36 4.76 -1.22 1.12 -7.46 -0.80 0.72 1.51 1.66 2.00
Nucleus 5.37 -35.99 -1.72 -7.58 35.50 3.60 -9.25 -4.19 -6.89 8.56 6.16 -38.15 -4.77 -10.09 36.59
Peroxisomes -4.76 6.39 -8.45 -6.47 -10.38 -6.69 5.28 -5.35 1.16 -7.77 -8.40 10.60 -9.95 -4.81 -13.07
Vacuole -0.96 -16.64 -7.27 -7.65 11.47 0.05 -10.22 -18.71 -18.68 5.29 0.79 -17.94 -17.57 -18.74 7.03
Vacuole Periphery -1.35 3.71 2.85 6.83 -2.83 1.27 13.79 3.78 2.63 -5.70 0.80 13.61 6.46 6.05 -6.14
Standard name
Human Ortholog
Description ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites; human homolog TRNT1 complements yeast null mutant
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Cca1

Cca1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cca1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available