Standard name
Human Ortholog
Description (SGD) Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 1.0 0.99 1.0 1.0 1.0 1.0 0.98 0.99 0.99 0.98 0.96 0.99 0.99 0.99 0.97 0.99 1.0 0.95 0.98 0.96 0.94 0.96 0.95
Endoplasmic Reticulum – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.11 0.07 0 0 0 0 0 0
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 1 0 0 1 0 4 3 1 2 6 2 1 5 1 1 2 3 0 0 0 0 0 0
Cytoplasm – 143 98 76 145 161 235 229 141 184 159 141 97 195 207 183 178 107 35 136 95 72 158 193
Endoplasmic Reticulum – 0 1 0 0 0 0 4 1 1 3 4 0 0 1 9 19 8 0 0 0 0 0 3
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0
Mitochondria – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 1 0 1 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Unique Cell Count 143 99 76 145 161 236 233 142 185 162 147 98 197 209 188 180 107 37 139 99 77 165 203
Labelled Cell Count 144 99 76 146 161 239 236 143 187 168 147 98 201 209 199 201 119 37 139 99 77 165 203


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 322.8 301.7 336.1 354.8 301.6 287.8 263.0 260.1 277.1 269.6 259.7 411.1 409.2 380.4 281.6 332.7 386.4 321.1 323.3 320.9
Std Deviation (1e-4) – 68.5 64.3 69.1 62.6 57.8 63.3 61.3 45.2 68.2 67.8 60.7 74.8 90.5 92.1 100.2 104.7 108.4 89.1 78.7 91.5
Intensity Change (Log2) – – – 0.16 0.23 -0.0 -0.07 -0.2 -0.21 -0.12 -0.16 -0.22 0.45 0.44 0.33 -0.1 0.14 0.36 0.09 0.1 0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.9 1.2 1.3 0.6 -0.5 0.3 0.5 -0.5 -1.4 0 0 0 -0.9 -0.1 1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 3.0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0019 0.0001 0.0005 0.0002 0.0003 0.0072 0.0093 0.0002 0.0067 0.0001 0.0012 0.0006 – – – – – –
Bud 0.0005 0.0002 0.0006 0.0001 0.0158 0.0141 0.0022 0.0002 0.002 0.0001 0.0005 0.0005 – – – – – –
Bud Neck 0.0001 0.0001 0.0002 0.0009 0.0027 0.0028 0.0006 0.0001 0.0006 0.0001 0.0002 0.0003 – – – – – –
Bud Periphery 0.0002 0 0.0004 0 0.0033 0.0129 0.0008 0.0001 0.001 0 0.0003 0.0005 – – – – – –
Bud Site 0.0001 0 0.0005 0 0.0019 0.0006 0.0005 0.0002 0.0018 0 0.001 0.0001 – – – – – –
Cell Periphery 0.0002 0 0.0038 0 0.0035 0.0006 0.0007 0.0001 0.0033 0 0.0023 0.0005 – – – – – –
Cytoplasm 0.7431 0.9941 0.6665 0.9913 0.2255 0.498 0.794 0.963 0.829 0.9959 0.7485 0.8517 – – – – – –
Cytoplasmic Foci 0.0003 0.0001 0.0079 0.0001 0.0769 0.0014 0.0065 0.0002 0.0006 0 0.0055 0.0003 – – – – – –
Eisosomes 0 0 0.0012 0 0.0002 0 0.0002 0.0001 0.0021 0 0.0039 0 – – – – – –
Endoplasmic Reticulum 0.0003 0 0.0001 0.0001 0.0005 0.0037 0.0015 0 0.0002 0 0.0001 0.0001 – – – – – –
Endosome 0.0005 0 0.0006 0 0.0014 0.0095 0.0114 0 0.0005 0 0.0002 0.0003 – – – – – –
Golgi 0 0 0.0003 0 0.0002 0.0004 0.0018 0 0.0002 0 0.0002 0.0001 – – – – – –
Lipid Particles 0 0 0.0069 0 0.0014 0.0001 0.0055 0 0.0007 0 0.0052 0.0001 – – – – – –
Mitochondria 0.0134 0.0003 0.1412 0.0002 0.1531 0.0128 0.0117 0.0302 0.0589 0.0001 0.1294 0.1023 – – – – – –
None 0.0029 0.0002 0.0089 0.0001 0.0947 0.0028 0.0121 0.0002 0.002 0.0001 0.0032 0.0005 – – – – – –
Nuclear Periphery 0.074 0.0005 0.0014 0.0013 0.0019 0.1331 0.0502 0.0002 0.0097 0.0002 0.0005 0.0013 – – – – – –
Nucleolus 0.0037 0 0.0991 0 0.1535 0.0023 0.0046 0.0013 0.0134 0 0.0759 0.0045 – – – – – –
Nucleus 0.1468 0.0042 0.0122 0.0056 0.2005 0.1967 0.063 0.0032 0.0473 0.0034 0.0093 0.0105 – – – – – –
Peroxisomes 0.0001 0 0.0038 0 0.0044 0.0012 0.0018 0.0004 0.0024 0 0.0036 0.0004 – – – – – –
Punctate Nuclear 0.0002 0 0.0002 0 0.0025 0.0015 0.004 0 0.0002 0 0.0003 0.0001 – – – – – –
Vacuole 0.0016 0 0.0133 0.0001 0.0328 0.0419 0.0025 0.0001 0.0066 0 0.0027 0.0036 – – – – – –
Vacuole Periphery 0.0099 0 0.0305 0.0001 0.023 0.0565 0.0152 0.0003 0.0108 0 0.0059 0.022 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5559.235 5704.3125 6612.2392 5958.9495 6301.1657 6058.3944 6199.2673 5803.0844 6765.1497 5909.4939
Translational Efficiency 1.505 1.54 1.4475 1.473 1.4786 1.4128 1.5977 1.739 1.5462 1.762

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Select an organelle or subcellular structure:


Penetrance Data

Penetrance values (percentage of cells with morphologically abnormal structure) at each time point for each of the subcellular structures monitored.

TP0 TP3 TP6 TP9 TP12 TP15 TP18 TP21 TP24
Autophagosome (ATG8) 21.276596 20.618557 56.984274 52.475248 53.975364 53.095975 51.727232 35.070671 41.932584
Septin (CDC11) 14.956012 21.504113 38.838839 50.716846 68.797400 65.407407 65.486726 61.054173 52.643336
Spindle & SPB (DAD2) 24.908425 28.417266 21.238177 18.561710 16.389812 22.100656 65.109489 51.532033 61.538462
P-bodies (DCP2) 16.666667 36.262100 44.506259 41.370011 30.499325 28.240741 23.305589 19.735099 30.046674
Inner Nuclear Membrane (HEH2) 14.846154 14.654688 20.472741 36.808283 19.587156 29.979798 13.148071 13.337357 19.415532
Metacaspase (MCA1) 8.333333 11.854685 28.865979 44.310475 63.527653 63.831361 47.776940 41.750842 40.328467
Plasma Membrane (MID2) 70.769231 33.841463 43.440860 46.567164 54.010695 69.539474 58.924303 37.683524 24.584718
Necrosis Factor (NHP6A) 17.857143 23.588342 30.780243 39.439907 48.714479 53.435115 53.710507 47.687642 32.434641
Nucleolus (NOP10) 29.513889 100.000000 43.905325 55.154639 73.992995 72.720000 62.517986 53.025719 47.905028
Kinetochore (NUF2) 22.018349 30.122324 33.664596 28.915663 24.132492 30.395913 32.484961 24.336283 22.792150
Mitochondria (OM45) 34.217507 45.219819 39.649321 40.128617 38.149556 37.589158 26.051980 24.956672 27.796804
Eisosomes (PIL1) 13.709677 17.169069 24.694056 29.816036 39.593346 51.547231 32.355680 46.460905 37.603306
Proteasome (PRE2) 56.756757 24.637681 51.449275 65.467626 87.074830 99.333333 100.000000 84.112150 91.509434
DNA Damage Foci (RAD52) 16.507177 14.076782 13.526912 14.093206 20.474777 22.704225 21.518231 23.033708 22.210125
Actin Patches (SAC6) 25.051760 32.112676 51.282051 68.289921 77.301162 87.689713 86.606335 67.053999 48.368146
Golgi, Early (SEC21) 19.062027 25.094697 25.957728 27.608069 31.556107 44.069069 55.555556 57.187500 53.628319
Golgi, Late (SEC7) 24.191750 30.168067 34.562500 32.351648 36.422501 41.379310 31.132917 23.005093 18.755847
Endosomes (SNF7) 25.386779 30.791962 45.532032 45.398601 49.885845 50.044497 48.131803 35.044707 44.955992
Endoplasmic Reticulum (SPF1) 25.647900 28.062016 42.422360 42.916450 36.617100 29.362494 30.050855 22.594142 27.091255
Lipid Droplets (TGL3) 15.015385 15.191200 24.544605 32.035794 39.580544 41.360759 46.030020 38.120404 36.395594
Vacuole (VPH1) 29.109589 25.263158 33.020344 49.797206 52.172196 58.782718 35.823951 26.727411 31.429549

Micrographs

Cell Count

Number of cells analysed for each strain. R1, R2: biological replicates; R1 & R2: both replicates combined.

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1753 178 1247 1259 234 517 1353 1339 1987 695 2600 2598

Protein Abundance

Mean and standard deviation of cell GFP intensity, and intensity change (log2) of each mutant relative to wild type.

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 20085.15 24762.52 22797.33 33396.30 22308.88 23054.79 24641.03 31165.58 20347.03 23492.17 23756.76 32246.59
Standard Deviation 4271.81 5109.41 4271.48 8721.32 4283.79 5407.69 5256.37 8317.45 4332.92 5384.72 4896.65 8588.22
Intensity Change Log 2 — 0.302029 0.182736 0.733559 — 0.047448 0.143444 0.482335 — 0.173672 0.162193 0.606821

Localization Score (DeepLoc)

Localization score for each compartment. Values highlighted in green denote significant localization scores (marked * in the plot).

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000013 0.000113 0.000064 0.000293 0.000109 0.000116 0.000060 0.000083 0.000025 0.000115 0.000062 0.000185
Bud Neck 0.000104 0.001825 0.001100 0.001210 0.001854 0.001441 0.000664 0.001203 0.000310 0.001539 0.000873 0.001206
Bud Site 0.000641 0.001951 0.001812 0.006106 0.001973 0.002429 0.002205 0.003281 0.000798 0.002307 0.002016 0.004650
Cell Periphery 0.001273 0.017277 0.012837 0.023397 0.021045 0.011564 0.005472 0.004836 0.003601 0.013027 0.009004 0.013831
Cytoplasm 0.940222* 0.789125* 0.828909* 0.708427* 0.777278* 0.786635* 0.860781* 0.779284* 0.921033* 0.787273* 0.845495* 0.744946*
Cytoplasmic Foci 0.001072 0.017075 0.004857 0.002944 0.015095 0.016361 0.003554 0.002852 0.002723 0.016544 0.004179 0.002896
Eisosomes 0.000000 0.000005 0.000002 0.000011 0.000005 0.000003 0.000001 0.000002 0.000001 0.000003 0.000002 0.000007
Endoplasmic Reticulum 0.000016 0.000018 0.000077 0.000137 0.000030 0.000028 0.000035 0.000039 0.000018 0.000025 0.000055 0.000087
Endosome 0.000016 0.000068 0.000091 0.000081 0.000090 0.000093 0.000071 0.000098 0.000025 0.000086 0.000081 0.000090
Golgi 0.001637 0.002467 0.004735 0.000579 0.004562 0.003422 0.001789 0.000678 0.001981 0.003178 0.003202 0.000630
Lipid Particles 0.000311 0.007522 0.007709 0.008862 0.008490 0.006267 0.002821 0.002745 0.001275 0.006589 0.005166 0.005709
Mitochondria 0.000063 0.000185 0.000082 0.000480 0.000384 0.000350 0.000103 0.000155 0.000100 0.000308 0.000093 0.000312
Mitotic Spindle 0.000062 0.000922 0.000268 0.000176 0.000479 0.000479 0.000048 0.000133 0.000111 0.000593 0.000153 0.000153
None 0.014368 0.006515 0.003033 0.006711 0.017396 0.008664 0.003270 0.006943 0.014725 0.008114 0.003156 0.006831
Nuclear Periphery 0.000049 0.000722 0.000565 0.000168 0.000798 0.000606 0.000182 0.000145 0.000137 0.000636 0.000366 0.000156
Nuclear Periphery Foci 0.000771 0.002541 0.003096 0.001255 0.003459 0.002929 0.001103 0.000894 0.001087 0.002830 0.002059 0.001069
Nucleolus 0.000029 0.000076 0.000059 0.000225 0.000160 0.000129 0.000096 0.000271 0.000044 0.000116 0.000078 0.000248
Nucleus 0.032700 0.103704 0.051576 0.049752 0.089187 0.117506 0.049595 0.077791 0.039352 0.113971 0.050545 0.064203
Peroxisomes 0.000007 0.000013 0.000008 0.000040 0.000015 0.000012 0.000018 0.000034 0.000008 0.000013 0.000013 0.000037
Vacuole 0.004559 0.046573 0.072789 0.187564 0.053015 0.039107 0.065277 0.117108 0.010266 0.041019 0.068880 0.151251
Vacuole Periphery 0.002086 0.001301 0.006330 0.001582 0.004575 0.001858 0.002856 0.001428 0.002379 0.001716 0.004522 0.001503

Localization Changes (T-score)

Change in localization of each mutant relative to its control (e.g. UBP2_WT: ubp2Δ vs wild type). Values highlighted in yellow (increase) or blue (decrease) denote significant changes (marked * in the plot).

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.86 -12.96 -4.59 -1.88 -3.23 -0.38 1.34 0.83 1.84 -1.32 -6.26 -7.30 -5.70 -1.04 -3.46
Bud Neck -4.29 -5.79 -12.88 1.72 0.13 0.32 1.60 -0.66 -1.43 -4.59 -5.90 -6.29 -12.30 0.21 -3.31
Bud Site -3.57 -3.05 -6.47 -4.55 -3.93 -0.73 -0.72 -2.60 -1.35 -2.06 -2.86 -4.36 -7.92 -3.15 -4.32
Cell Periphery -6.82 -11.84 -12.41 0.31 -2.66 2.78 4.90 5.43 5.80 2.49 -7.87 -7.86 -8.57 2.21 -1.53
Cytoplasm 11.26 19.79 31.13 1.05 9.02 0.00 -4.26 1.31 1.97 11.74 18.30 19.65 35.11 2.70 14.58
Cytoplasmic Foci -6.50 -9.53 -5.76 5.54 3.32 -0.96 4.22 4.21 7.35 0.04 -10.13 -5.38 -2.99 9.27 2.40
Eisosomes -4.75 -8.74 -3.16 -0.43 -2.28 1.58 2.63 2.14 1.98 -2.86 -7.04 -5.23 -3.57 -0.77 -2.57
Endoplasmic Reticulum -1.10 -2.72 -2.55 -2.42 -0.48 0.57 -0.52 0.12 -0.78 0.89 -2.06 -3.49 -3.00 -2.34 -0.29
Endosome -3.50 -8.27 -6.97 0.02 2.06 0.15 1.00 -0.48 -0.64 -2.41 -3.80 -9.02 -8.46 -0.14 -0.55
Golgi -2.89 -8.34 4.72 7.16 13.48 1.49 4.92 7.34 4.53 7.64 -2.52 -4.94 6.40 5.78 15.28
Lipid Particles -5.78 -12.11 -10.58 0.52 0.43 1.21 3.21 3.09 3.44 -0.36 -7.47 -10.19 -9.25 1.97 0.16
Mitochondria -1.62 -3.03 -2.64 -0.93 -2.34 -0.09 1.40 1.40 2.25 -0.05 -2.69 -0.48 -2.35 1.14 -2.24
Mitotic Spindle -1.36 -3.87 -1.47 1.18 1.34 0.25 2.44 1.87 2.02 -1.95 -2.50 -1.50 -1.14 2.18 0.06
None 6.97 11.32 6.41 0.35 -2.21 2.41 3.90 3.28 2.65 -1.35 6.80 11.41 7.98 2.68 -2.43
Nuclear Periphery -6.83 -9.10 -13.08 5.77 6.99 0.86 4.35 4.49 5.17 0.43 -8.22 -7.89 -3.77 7.41 6.51
Nuclear Periphery Foci -5.13 -9.52 -5.05 3.42 6.95 0.79 4.09 4.17 4.42 0.39 -5.94 -7.06 -2.23 5.23 6.25
Nucleolus -4.67 -6.94 -5.14 -3.10 -4.02 0.03 0.80 -1.37 -1.59 -4.09 -2.61 -5.05 -7.46 -1.99 -5.72
Nucleus -8.68 -9.61 -8.98 5.89 0.15 -3.02 4.51 -0.97 2.95 -9.68 -16.00 -8.30 -14.45 7.42 -7.72
Peroxisomes -1.55 -1.06 -6.27 -3.81 -6.28 0.06 -0.92 -1.96 -2.06 -1.08 -1.86 -2.65 -6.93 -3.76 -4.57
Vacuole -6.83 -17.39 -27.99 -9.16 -11.81 0.97 -1.95 -6.34 -10.92 -7.39 -9.45 -21.65 -33.69 -15.91 -13.68
Vacuole Periphery 0.99 -4.13 1.40 0.76 5.89 2.18 1.45 3.00 1.89 5.27 0.93 -3.13 2.23 1.92 7.44
Standard name
Human Ortholog
Description (SGD) Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Cdc19

Cdc19


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc19-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available