Standard name | |
---|---|
Human Ortholog | |
Description | DNA ligase I found in nucleus and mitochondria; essential enzyme that joins Okazaki fragments during DNA replication; also acts in ribonucleotide excision repair, base excision repair, and recombination; DNA ligase I mutants trigger ubiquitination of PCNA at K107, facilitating Rad59p-mediated bypass of unligated Okazaki fragments; human homolog LIG1 can complement yeast cdc9 temperature-sensitive mutant at restrictive temperature |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 5 | 6 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 1 | 10 | 5 | 10 | 16 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 2 | 4 | 12 | 6 | 7 | 6 | 11 | 7 | 5 | 7 | 3 | 5 | 11 | 13 | 10 | 11 | 5 | 1 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 3 | 1 | 0 | 0 | 1 | 1 | 1 | 4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 7 | 5 | 0 | 1 | 0 | 1 | 0 | 3 | 0 | 2 | 6 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 2 | 2 | 2 | 3 | 0 |
Mitochondria | 4 | 10 | 4 | 18 | 9 | 52 | 80 | 49 | 113 | 118 | 127 | 108 | 10 | 11 | 19 | 0 | 1 | 3 | 1 | 2 | 2 | 2 | 12 | 20 |
Nucleus | 159 | 179 | 248 | 238 | 281 | 259 | 388 | 398 | 356 | 389 | 259 | 371 | 239 | 224 | 218 | 69 | 57 | 34 | 128 | 165 | 208 | 49 | 87 | 89 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 4 | 4 | 6 | 5 | 2 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 |
Nucleolus | 0 | 2 | 0 | 3 | 0 | 4 | 13 | 23 | 27 | 42 | 42 | 27 | 0 | 5 | 2 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 21 | 46 | 25 | 16 | 16 | 29 | 17 | 18 | 6 | 18 | 7 | 9 | 12 | 17 | 30 | 21 | 14 | 9 | 56 | 69 | 62 | 44 | 129 | 154 |
Unique Cell Count | 183 | 222 | 267 | 272 | 305 | 316 | 446 | 475 | 428 | 481 | 338 | 437 | 261 | 266 | 273 | 98 | 87 | 51 | 190 | 246 | 284 | 107 | 248 | 290 |
Labelled Cell Count | 190 | 239 | 282 | 290 | 312 | 355 | 512 | 509 | 524 | 588 | 463 | 539 | 271 | 280 | 292 | 102 | 90 | 55 | 190 | 246 | 284 | 107 | 248 | 290 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.4 | 8.9 | 7.8 | 7.5 | 7.9 | 6.4 | 6.6 | 6.9 | 6.0 | 5.9 | 5.3 | 5.8 | 8.6 | 8.8 | 8.6 | 14.0 | 15.8 | 13.5 | 7.8 | 7.4 | 7.7 |
Std Deviation (1e-4) | 2.2 | 2.2 | 1.4 | 1.6 | 1.4 | 1.2 | 1.4 | 1.5 | 1.4 | 1.3 | 1.2 | 1.1 | 2.3 | 2.6 | 2.8 | 4.0 | 6.2 | 3.1 | 2.0 | 1.3 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.05 | 0.02 | -0.28 | -0.24 | -0.19 | -0.37 | -0.4 | -0.55 | -0.43 | 0.15 | 0.17 | 0.14 | 0.85 | 1.02 | 0.79 | -0.01 | -0.08 | -0.03 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.0 | 0 | 0.6 | 0 | 0.8 | 0 | 0 | 0 | 0 | 0 | 1.8 | 2.2 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.0 | 1.2 | 6.1 | 6.6 | 4.5 | 8.5 | 8.2 | 10.7 | 8.2 | 1.7 | 1.8 | 3.1 | 0 | 0 | 0 |
Nucleus | -2.1 | -0.3 | -3.9 | -2.5 | -3.5 | -3.7 | -4.4 | -5.4 | -3.2 | -0.6 | -3.1 | -4.4 | -5.6 | -6.4 | -5.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 3.7 | 4.2 | 5.0 | 6.0 | 4.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.5 | -1.9 | -0.1 | -3.0 | -3.1 | -4.9 | -3.2 | -4.0 | -4.4 | -2.1 | -1.3 | 0.6 | 3.1 | 1.7 | 1.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.5754 | 4.7884 | 4.7903 | 3.4952 | 3.5575 | 4.3284 | 3.975 | 5.9638 | 5.9134 | 5.3132 | 3.9491 | 5.0874 | 4.218 | 5.6392 | 5.5029 | 4.9255 | 4.419 | 4.7102 |
Actin | 0.0654 | 0.0001 | 0.0035 | 0.0114 | 0.0459 | 0.0214 | 0.0037 | 0.0001 | 0.006 | 0.0106 | 0.0016 | 0.0002 | 0.028 | 0.0001 | 0.0066 | 0.0006 | 0.0001 | 0.0002 |
Bud | 0.0009 | 0.0002 | 0.0005 | 0.0022 | 0.0033 | 0.0006 | 0.0004 | 0.0001 | 0.0006 | 0.0002 | 0.0007 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0.0001 |
Bud Neck | 0.0037 | 0.0002 | 0.0006 | 0.0003 | 0.0033 | 0.0017 | 0.0007 | 0.0002 | 0.0022 | 0.0002 | 0.0014 | 0.0022 | 0.0024 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0032 |
Bud Periphery | 0.0022 | 0.0004 | 0.0011 | 0.0013 | 0.005 | 0.0012 | 0.0008 | 0.0006 | 0.0009 | 0.0005 | 0.0054 | 0.0004 | 0.0007 | 0.0001 | 0.0003 | 0.0014 | 0.0002 | 0.0004 |
Bud Site | 0.0028 | 0.0006 | 0.0106 | 0.0049 | 0.049 | 0.0012 | 0.0006 | 0.0001 | 0.0038 | 0.0005 | 0.0006 | 0.0001 | 0.0042 | 0.0001 | 0.0003 | 0.0004 | 0.0001 | 0.0001 |
Cell Periphery | 0.0006 | 0.0002 | 0.0011 | 0.0001 | 0.0011 | 0.0002 | 0.0002 | 0.0003 | 0.0004 | 0.0002 | 0.0012 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0002 |
Cytoplasm | 0.0072 | 0.0003 | 0.0021 | 0.0062 | 0.0099 | 0.0006 | 0.0022 | 0.0003 | 0.0009 | 0.0006 | 0.0006 | 0.0003 | 0.0026 | 0.0004 | 0.0002 | 0.001 | 0.0002 | 0.0002 |
Cytoplasmic Foci | 0.0333 | 0.0002 | 0.004 | 0.0224 | 0.0342 | 0.0035 | 0.02 | 0 | 0.0067 | 0.0095 | 0.0001 | 0 | 0.0175 | 0 | 0.0018 | 0.0002 | 0 | 0.0002 |
Eisosomes | 0.001 | 0 | 0.0002 | 0 | 0.0004 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0089 | 0 | 0.0013 | 0.0004 | 0.0119 | 0.0039 | 0.0043 | 0.0002 | 0.0004 | 0.001 | 0.0024 | 0.0003 | 0.0046 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0001 |
Endosome | 0.0495 | 0.0004 | 0.0058 | 0.0181 | 0.0713 | 0.0114 | 0.0317 | 0.0002 | 0.0061 | 0.003 | 0.008 | 0.0002 | 0.0206 | 0 | 0.0017 | 0.0019 | 0.0001 | 0.0007 |
Golgi | 0.0167 | 0.0001 | 0.0016 | 0.0122 | 0.0112 | 0.0091 | 0.0108 | 0 | 0.0074 | 0.0044 | 0.0006 | 0 | 0.0087 | 0 | 0.0036 | 0.0005 | 0 | 0.0004 |
Lipid Particles | 0.0401 | 0.0007 | 0.009 | 0.0012 | 0.0143 | 0.0058 | 0.0249 | 0 | 0.0103 | 0.021 | 0.0009 | 0 | 0.0151 | 0 | 0.0013 | 0.0003 | 0 | 0.0026 |
Mitochondria | 0.0199 | 0.0086 | 0.013 | 0.0041 | 0.0452 | 0.0091 | 0.0072 | 0.0042 | 0.0152 | 0.0209 | 0.0369 | 0.0015 | 0.0061 | 0.0017 | 0.0144 | 0.059 | 0.0033 | 0.007 |
None | 0.0095 | 0 | 0.0024 | 0.0003 | 0.0029 | 0.0003 | 0.0006 | 0.0001 | 0.0004 | 0.0005 | 0.0005 | 0 | 0.0024 | 0 | 0 | 0.0001 | 0.0001 | 0 |
Nuclear Periphery | 0.0284 | 0.0052 | 0.0087 | 0.0014 | 0.0431 | 0.0148 | 0.0492 | 0.012 | 0.0076 | 0.0205 | 0.0652 | 0.0067 | 0.0175 | 0.0106 | 0.0047 | 0.01 | 0.0071 | 0.0026 |
Nucleolus | 0.0144 | 0.013 | 0.0079 | 0.0074 | 0.0421 | 0.0184 | 0.0121 | 0.0146 | 0.0066 | 0.0058 | 0.0429 | 0.0189 | 0.0097 | 0.0103 | 0.0073 | 0.0104 | 0.0156 | 0.0253 |
Nucleus | 0.6545 | 0.9657 | 0.9153 | 0.8992 | 0.5552 | 0.8887 | 0.8023 | 0.9587 | 0.9125 | 0.8755 | 0.7927 | 0.9664 | 0.8199 | 0.9752 | 0.9527 | 0.9026 | 0.9708 | 0.9533 |
Peroxisomes | 0.01 | 0.0004 | 0.0048 | 0.0051 | 0.0048 | 0.0018 | 0.0147 | 0 | 0.0072 | 0.013 | 0.0002 | 0 | 0.0245 | 0 | 0.0018 | 0.0003 | 0 | 0.0003 |
Punctate Nuclear | 0.0211 | 0.0002 | 0.0016 | 0.0007 | 0.006 | 0.0031 | 0.0047 | 0.0005 | 0.0028 | 0.008 | 0.001 | 0.0003 | 0.0024 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0002 |
Vacuole | 0.0063 | 0.002 | 0.0034 | 0.0007 | 0.0219 | 0.0013 | 0.0037 | 0.0035 | 0.0011 | 0.0013 | 0.016 | 0.0014 | 0.0093 | 0.0004 | 0.001 | 0.005 | 0.0005 | 0.0016 |
Vacuole Periphery | 0.0033 | 0.0016 | 0.0015 | 0.0004 | 0.018 | 0.0015 | 0.0054 | 0.0043 | 0.0009 | 0.0027 | 0.0209 | 0.0008 | 0.0031 | 0.0005 | 0.0015 | 0.0047 | 0.0014 | 0.0013 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 42.5696 | 10.0029 | 10.3271 | 24.3676 | 36.2699 | 37.9268 | 12.9722 | 12.9105 | 19.5978 | 39.5739 |
Translational Efficiency | 1.678 | 1.4698 | 1.1595 | 1.2546 | 1.2432 | 1.8195 | 0.8955 | 0.7498 | 0.9503 | 1.0003 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1866 | 706 | 1318 | 1154 | 735 | 1855 | 1553 | 445 | 2601 | 2561 | 2871 | 1599 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 760.86 | 934.24 | 1263.74 | 1263.32 | 851.96 | 976.96 | 1189.96 | 1356.01 | 786.60 | 965.18 | 1223.83 | 1289.12 |
Standard Deviation | 108.20 | 173.08 | 200.39 | 217.50 | 124.33 | 148.80 | 174.82 | 250.31 | 120.20 | 157.03 | 190.57 | 230.88 |
Intensity Change Log 2 | — | 0.296162 | 0.731997 | 0.731517 | — | 0.197514 | 0.482055 | 0.670510 | — | 0.244893 | 0.605373 | 0.699612 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000981 | 0.004662 | 0.002356 | 0.012317 | 0.003449 | 0.002933 | 0.002839 | 0.019465 | 0.001678 | 0.003410 | 0.002618 | 0.014306 |
Bud Neck | 0.016563 | 0.039604 | 0.046055 | 0.197914* | 0.028500 | 0.039735 | 0.057138 | 0.185816* | 0.019937 | 0.039699 | 0.052050 | 0.194547* |
Bud Site | 0.006271 | 0.012455 | 0.003426 | 0.053441 | 0.009529 | 0.009001 | 0.006378 | 0.055315 | 0.007191 | 0.009953 | 0.005023 | 0.053962 |
Cell Periphery | 0.000627 | 0.000579 | 0.000201 | 0.000745 | 0.000638 | 0.000544 | 0.000286 | 0.000774 | 0.000630 | 0.000553 | 0.000247 | 0.000753 |
Cytoplasm | 0.015488 | 0.003713 | 0.001071 | 0.006434 | 0.002135 | 0.001828 | 0.001688 | 0.004042 | 0.011715 | 0.002347 | 0.001405 | 0.005768 |
Cytoplasmic Foci | 0.005945 | 0.001360 | 0.000075 | 0.004394 | 0.002115 | 0.001348 | 0.000393 | 0.001008 | 0.004863 | 0.001351 | 0.000247 | 0.003452 |
Eisosomes | 0.000049 | 0.000120 | 0.000060 | 0.000145 | 0.000097 | 0.000105 | 0.000071 | 0.000203 | 0.000063 | 0.000109 | 0.000066 | 0.000161 |
Endoplasmic Reticulum | 0.021098 | 0.036982 | 0.008479 | 0.030416 | 0.041705 | 0.060467 | 0.022434 | 0.034796 | 0.026921 | 0.053993 | 0.016028 | 0.031635 |
Endosome | 0.001641 | 0.001871 | 0.000312 | 0.006267 | 0.001697 | 0.002207 | 0.000722 | 0.001541 | 0.001657 | 0.002115 | 0.000534 | 0.004952 |
Golgi | 0.001420 | 0.001297 | 0.000087 | 0.008653 | 0.000724 | 0.001349 | 0.000124 | 0.000396 | 0.001224 | 0.001335 | 0.000107 | 0.006355 |
Lipid Particles | 0.003201 | 0.001683 | 0.000128 | 0.001768 | 0.002930 | 0.001756 | 0.000330 | 0.001551 | 0.003124 | 0.001736 | 0.000237 | 0.001707 |
Mitochondria | 0.019111 | 0.032647 | 0.005697 | 0.057977 | 0.031298 | 0.026481 | 0.009007 | 0.042524 | 0.022555 | 0.028181 | 0.007488 | 0.053676 |
Mitotic Spindle | 0.004102 | 0.008022 | 0.001242 | 0.049996 | 0.008149 | 0.004615 | 0.004145 | 0.028967 | 0.005246 | 0.005554 | 0.002812 | 0.044144 |
None | 0.013566 | 0.005042 | 0.000890 | 0.002374 | 0.003692 | 0.002771 | 0.001613 | 0.002801 | 0.010775 | 0.003397 | 0.001281 | 0.002493 |
Nuclear Periphery | 0.003431 | 0.002077 | 0.001358 | 0.007024 | 0.004486 | 0.005199 | 0.002984 | 0.003579 | 0.003729 | 0.004338 | 0.002237 | 0.006066 |
Nuclear Periphery Foci | 0.001264 | 0.000793 | 0.000123 | 0.002843 | 0.001713 | 0.001472 | 0.000958 | 0.002118 | 0.001391 | 0.001285 | 0.000575 | 0.002641 |
Nucleolus | 0.017705 | 0.021815 | 0.007045 | 0.011367 | 0.027846 | 0.020392 | 0.008435 | 0.010815 | 0.020571 | 0.020785 | 0.007797 | 0.011213 |
Nucleus | 0.853687* | 0.803532* | 0.917399* | 0.505934* | 0.810098* | 0.796649* | 0.874174* | 0.576740* | 0.841369* | 0.798547* | 0.894018* | 0.525639* |
Peroxisomes | 0.005769 | 0.007103 | 0.000714 | 0.003809 | 0.009421 | 0.005065 | 0.001483 | 0.006306 | 0.006801 | 0.005627 | 0.001130 | 0.004504 |
Vacuole | 0.005667 | 0.010803 | 0.002744 | 0.029288 | 0.006844 | 0.012833 | 0.004280 | 0.018207 | 0.006000 | 0.012274 | 0.003575 | 0.026204 |
Vacuole Periphery | 0.002414 | 0.003840 | 0.000538 | 0.006895 | 0.002933 | 0.003249 | 0.000517 | 0.003036 | 0.002561 | 0.003412 | 0.000527 | 0.005821 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -9.81 | -2.80 | -11.84 | -7.50 | -8.97 | 1.72 | 1.09 | -7.34 | -7.66 | -7.56 | -9.46 | -4.05 | -13.70 | -11.76 | -12.04 |
Bud Neck | -7.24 | -12.65 | -32.24 | -25.67 | -25.37* | -3.48 | -9.15 | -18.82 | -17.86 | -15.00 | -10.00 | -17.20 | -37.30 | -32.40 | -28.91 |
Bud Site | -4.27 | 3.08 | -18.69 | -14.63 | -20.09 | 0.51 | 1.90 | -10.81 | -11.22 | -11.64 | -3.47 | 2.19 | -21.53 | -20.23 | -22.66 |
Cell Periphery | 0.48 | 6.40 | -0.99 | -1.43 | -10.23 | 0.65 | 2.54 | -0.93 | -3.11 | -7.06 | 1.11 | 6.24 | -1.35 | -3.44 | -11.98 |
Cytoplasm | 6.54 | 11.47 | 6.64 | -2.12 | -11.91 | 0.48 | 0.92 | -3.14 | -3.69 | -4.77 | 9.14 | 11.20 | 5.87 | -6.19 | -12.23 |
Cytoplasmic Foci | 5.30 | 7.32 | 2.89 | -2.56 | -5.08 | 1.49 | 3.26 | 2.16 | 0.84 | -2.18 | 5.59 | 7.66 | 3.26 | -2.42 | -5.06 |
Eisosomes | -8.48 | -3.84 | -13.40 | -2.29 | -10.44 | -1.15 | 4.20 | -6.95 | -6.65 | -8.83 | -11.28 | -2.21 | -14.59 | -7.13 | -13.54 |
Endoplasmic Reticulum | -7.44 | 11.37 | -5.19 | 2.57 | -12.91 | -6.44 | 7.61 | 1.63 | 7.20 | -4.14 | -15.71 | 9.27 | -3.05 | 10.87 | -10.12 |
Endosome | -0.84 | 10.82 | -4.96 | -4.23 | -7.03 | -1.76 | 3.95 | -0.00 | 2.09 | -5.22 | -2.53 | 9.49 | -4.88 | -3.77 | -7.29 |
Golgi | 0.16 | 3.48 | -2.84 | -2.83 | -3.81 | -1.17 | 2.89 | 1.40 | 1.88 | -6.15 | -0.28 | 3.98 | -2.76 | -2.57 | -3.87 |
Lipid Particles | 1.94 | 6.23 | 2.33 | -0.32 | -6.83 | 1.87 | 4.58 | 1.61 | -0.60 | -7.05 | 2.92 | 7.41 | 2.78 | -0.56 | -8.42 |
Mitochondria | -3.19 | 7.05 | -10.26 | -4.17 | -17.46 | 1.27 | 6.47 | -2.44 | -4.82 | -12.08 | -2.44 | 8.91 | -10.19 | -7.99 | -20.14 |
Mitotic Spindle | -1.61 | 2.80 | -9.23 | -7.55 | -9.93 | 1.74 | 1.47 | -3.49 | -4.23 | -4.13 | -0.23 | 1.80 | -9.79 | -9.60 | -10.36 |
None | 5.83 | 11.02 | 9.89 | 3.12 | -7.35 | 1.31 | 3.35 | 1.77 | 0.67 | -1.78 | 8.02 | 11.14 | 9.98 | 3.01 | -4.68 |
Nuclear Periphery | 2.54 | 4.37 | -4.20 | -6.06 | -7.15 | -0.69 | 1.37 | 0.41 | 1.91 | -1.97 | -1.15 | 3.14 | -3.66 | -3.00 | -6.61 |
Nuclear Periphery Foci | 1.67 | 5.46 | -4.48 | -5.87 | -8.77 | 0.38 | 1.21 | -1.26 | -1.72 | -2.25 | 0.23 | 2.64 | -4.23 | -4.50 | -6.12 |
Nucleolus | -1.84 | 8.03 | 4.62 | 5.05 | -3.72 | 3.20 | 8.51 | 6.80 | 5.41 | -1.48 | -0.08 | 11.08 | 7.68 | 8.00 | -3.53 |
Nucleus | 4.92 | -9.21 | 32.75 | 23.11 | 41.25 | 1.22 | -5.80 | 14.94 | 15.53 | 20.29 | 6.68 | -8.45 | 35.62 | 30.83 | 43.99 |
Peroxisomes | -0.98 | 5.88 | 2.56 | 3.56 | -6.13 | 3.20 | 6.38 | 2.44 | -0.68 | -4.53 | 1.40 | 7.90 | 3.49 | 2.25 | -6.80 |
Vacuole | -4.15 | 3.43 | -13.64 | -9.81 | -15.17 | -5.28 | 2.09 | -7.32 | -4.59 | -8.35 | -7.54 | 3.12 | -15.41 | -10.74 | -16.90 |
Vacuole Periphery | -1.87 | 4.59 | -2.93 | -1.23 | -5.42 | -0.44 | 3.48 | 0.36 | 1.02 | -3.64 | -1.97 | 5.84 | -2.61 | -1.48 | -6.18 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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