Standard name
Human Ortholog
Description Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 0.87 0.95 1.0 0.94 0.77 – 0.75 0.61 0.6 0.59 0.29 0.56 0.99 0.98 0.99 – – 0.64 0.59 0.75 0.47 0.8 0.75 0.7
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – 0.05 0 0 0.05 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0.24 0.07 0 0.06 0.31 – 0.48 0.52 0.45 0.52 0.72 0.6 0 0 0 – – 0.07 0.24 0.12 0.21 0.07 0.12 0.1
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0.06 0 0 0 0 0 0 0 – – 0.13 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.06 0 – 0 0 0 0 0 0 0 0 0 – – 0 0 0 0.06 0 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – 4 2 2 1 2 2 3
Bud 0 0 0 0 0 – 1 0 1 1 2 2 0 0 0 – – 1 0 0 0 1 4 6
Bud Neck 0 1 0 0 1 – 0 0 0 0 1 1 1 0 0 – – 3 3 3 1 1 2 2
Bud Site 1 0 0 0 0 – 0 1 0 0 1 0 0 0 0 – – 4 – – – – – –
Cell Periphery 0 2 0 0 0 – 0 0 1 0 0 2 0 0 1 – – 3 1 2 0 1 2 4
Cytoplasm 217 200 28 15 57 – 49 58 49 43 23 63 360 521 442 – – 78 158 167 21 163 209 203
Endoplasmic Reticulum 3 0 0 0 1 – 0 0 0 1 1 0 0 4 1 – – 9 5 1 0 0 0 1
Endosome 0 0 0 0 0 – 0 1 0 0 0 0 0 2 0 – – 6 7 4 2 2 2 4
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – 1 1 0 0 0 3 0
Mitochondria 60 15 0 1 23 – 31 49 37 38 56 68 4 4 2 – – 9 64 27 9 15 32 29
Nucleus 1 0 0 0 0 – 1 1 0 0 0 0 2 1 0 – – 2 0 0 0 0 0 1
Nuclear Periphery 1 1 0 0 0 – 0 0 0 0 1 1 0 0 1 – – 0 0 0 0 0 0 1
Nucleolus 1 0 0 0 1 – 1 0 0 1 2 0 0 1 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 2 0 0 0 0 – 0 6 3 2 2 1 0 2 1 – – 16 4 2 0 5 4 5
Vac/Vac Membrane 1 0 0 1 0 – 0 2 0 1 2 0 2 1 3 – – 3 9 5 2 2 7 19
Unique Cell Count 249 210 28 16 74 65 95 82 73 78 113 364 530 448 121 270 224 46 204 280 293
Labelled Cell Count 287 219 28 17 83 83 118 91 87 91 138 369 536 451 139 270 224 46 204 280 293


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.0 3.2 3.6 3.0 3.7 – 3.1 3.1 2.9 2.7 3.3 2.5 3.8 3.8 3.8 4.0 4.6 4.9 3.6 4.0 4.4
Std Deviation (1e-4) 0.4 0.7 2.0 1.1 2.3 – 2.1 1.5 1.8 1.6 2.3 1.3 1.0 1.5 1.3 1.3 1.6 1.3 1.4 1.9 1.7
Intensity Change (Log2) – – – -0.28 0.05 – -0.21 -0.23 -0.32 -0.43 -0.13 -0.56 0.07 0.06 0.05 0.13 0.33 0.43 -0.01 0.14 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0982 0.1453 0.1552 -0.2883 0.3552 0.2932 0.0849 -0.1235 -0.1951 -0.3848 -0.5368 -0.1913 0.2548 0.9571 0.2653 0.4475 0.2794 0.6562
Actin 0.0327 0.0032 0.0094 0.0008 0.0115 0.0099 0.0339 0.0005 0.0061 0.0017 0.0033 0.0107 0.0113 0.005 0.0155 0.0083 0.0049 0.0036
Bud 0.0007 0.0093 0.0036 0.0111 0.0033 0.0022 0.002 0.0002 0.0014 0.0016 0.0001 0.0009 0.0012 0.0011 0.0011 0.0001 0.0002 0.0006
Bud Neck 0.0046 0.0021 0.0046 0.0128 0.0038 0.0044 0.001 0.0001 0.001 0.0004 0.0001 0.0053 0.001 0.0008 0.0064 0.0012 0.0007 0.0027
Bud Periphery 0.0023 0.0092 0.0058 0.024 0.0091 0.0052 0.0099 0.0006 0.0029 0.0041 0.0004 0.0034 0.0031 0.0027 0.0056 0.0002 0.0005 0.0016
Bud Site 0.0063 0.0224 0.0062 0.0079 0.0031 0.0006 0.0028 0.001 0.0052 0.0014 0.0002 0.0005 0.0013 0.0151 0.0032 0.0003 0.0005 0.0004
Cell Periphery 0.001 0.0012 0.0008 0.0005 0.0007 0.0002 0.0025 0.0005 0.0012 0.0002 0.0002 0.0009 0.0003 0.002 0.0012 0.0002 0.0003 0.0004
Cytoplasm 0.0612 0.0874 0.0647 0.0917 0.0723 0.1421 0.0315 0.035 0.0438 0.0717 0.0415 0.0416 0.0715 0.1067 0.0835 0.0604 0.1134 0.121
Cytoplasmic Foci 0.039 0.0208 0.0387 0.0463 0.0153 0.0363 0.0339 0.0243 0.0354 0.0262 0.0165 0.0426 0.0334 0.0324 0.0343 0.0751 0.0357 0.049
Eisosomes 0.0007 0.0003 0.0007 0.0001 0.0004 0.0002 0.0011 0.0004 0.0015 0.0001 0.0004 0.0003 0.0004 0.0004 0.0007 0.0007 0.0009 0.0002
Endoplasmic Reticulum 0.0034 0.002 0.002 0.0022 0.0034 0.0011 0.0045 0.0008 0.0033 0.0005 0.0007 0.001 0.0023 0.0025 0.0015 0.0009 0.0008 0.0019
Endosome 0.0152 0.0138 0.0113 0.0135 0.0098 0.0098 0.0376 0.0023 0.0236 0.0344 0.0079 0.0134 0.0196 0.0165 0.0043 0.0153 0.0327 0.0155
Golgi 0.0065 0.0014 0.0037 0.0018 0.0038 0.0024 0.0181 0.0013 0.0062 0.0093 0.0083 0.003 0.0036 0.0029 0.0021 0.0022 0.0046 0.0052
Lipid Particles 0.0079 0.0039 0.0051 0.0032 0.0018 0.0036 0.039 0.0064 0.0056 0.0108 0.0222 0.0042 0.0071 0.0034 0.003 0.0042 0.0329 0.0038
Mitochondria 0.0102 0.0176 0.0091 0.006 0.0322 0.0037 0.0249 0.0153 0.0102 0.0051 0.0028 0.0203 0.0263 0.0151 0.0025 0.0016 0.0108 0.0306
None 0.7799 0.784 0.8043 0.7414 0.8015 0.7592 0.6932 0.9014 0.8371 0.7671 0.8852 0.8396 0.7904 0.7774 0.8185 0.8045 0.7426 0.7475
Nuclear Periphery 0.0032 0.0013 0.0012 0.0022 0.0053 0.0006 0.0032 0.0002 0.0009 0.0002 0.0004 0.0019 0.0049 0.001 0.0006 0.0004 0.0003 0.0003
Nucleolus 0.0009 0.002 0.0014 0.0063 0.0011 0.0004 0.0006 0.0004 0.002 0.0002 0.0002 0.0003 0.0012 0.0006 0.0004 0.0003 0.0006 0.0002
Nucleus 0.0014 0.0033 0.0019 0.0063 0.0078 0.001 0.0011 0.0004 0.0013 0.0004 0.0007 0.0008 0.0105 0.0008 0.0017 0.0004 0.0004 0.0005
Peroxisomes 0.0096 0.003 0.0144 0.0056 0.0037 0.0099 0.0511 0.0065 0.003 0.0606 0.0069 0.0045 0.0031 0.0067 0.0111 0.0217 0.0135 0.0093
Punctate Nuclear 0.0077 0.0038 0.0077 0.0067 0.0006 0.0052 0.0035 0.001 0.0014 0.0007 0.0012 0.0019 0.0048 0.0009 0.0014 0.0012 0.0005 0.0014
Vacuole 0.0047 0.0069 0.0027 0.0081 0.0069 0.0015 0.0032 0.0014 0.0059 0.0025 0.0008 0.0019 0.0019 0.005 0.0013 0.0007 0.0024 0.003
Vacuole Periphery 0.0009 0.001 0.0006 0.0014 0.0027 0.0005 0.0012 0.0001 0.0009 0.0009 0.0003 0.0009 0.0006 0.001 0.0002 0.0002 0.0009 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1.5105 2.7006 1.002 2.1034 1.4688 2.2645 3.2425 3.4555 4.329 2.9311
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1839 1266 174 1379 1112 1553 222 1163 2951 2819 396 2542

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 663.97 619.43 880.97 832.83 643.26 634.15 922.02 772.66 656.17 627.54 903.98 805.30
Standard Deviation 69.73 110.84 99.32 121.18 70.73 92.15 116.67 111.04 70.82 101.23 111.27 120.44
Intensity Change Log 2 — -0.100177 0.407975 0.326904 — -0.020578 0.519396 0.264432 — -0.060459 0.463878 0.296501

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000063 0.000276 0.000635 0.014794 0.000034 0.000374 0.000344 0.000888 0.000052 0.000330 0.000472 0.008432
Bud Neck 0.023210 0.034942 0.003452 0.027159 0.009222 0.066054 0.006208 0.026541 0.017939 0.052081 0.004997 0.026877
Bud Site 0.004871 0.008160 0.011595 0.112395* 0.001327 0.018701 0.007433 0.027736 0.003536 0.013967 0.009262 0.073662
Cell Periphery 0.000214 0.000238 0.000292 0.000247 0.000132 0.000180 0.000203 0.000344 0.000183 0.000206 0.000242 0.000291
Cytoplasm 0.526172* 0.333212* 0.224850* 0.259164* 0.515215* 0.293898* 0.268881* 0.328918* 0.522043* 0.311554* 0.249534* 0.291078*
Cytoplasmic Foci 0.110882 0.243775* 0.027192 0.090471 0.182191* 0.313579* 0.050291 0.086160 0.137753 0.282231* 0.040141 0.088499
Eisosomes 0.000150 0.000308 0.000117 0.000123 0.000112 0.000213 0.000107 0.000141 0.000136 0.000255 0.000111 0.000132
Endoplasmic Reticulum 0.000668 0.001187 0.008017 0.003187 0.000583 0.000437 0.003605 0.002548 0.000636 0.000774 0.005543 0.002894
Endosome 0.005485 0.022223 0.007294 0.022559 0.005293 0.042555 0.010770 0.044311 0.005413 0.033424 0.009242 0.032511
Golgi 0.000779 0.006191 0.000254 0.027737 0.000788 0.012184 0.000695 0.008583 0.000783 0.009492 0.000502 0.018974
Lipid Particles 0.005665 0.014998 0.005466 0.003283 0.006691 0.012394 0.003279 0.009358 0.006052 0.013563 0.004240 0.006062
Mitochondria 0.003061 0.023833 0.000592 0.035699 0.001585 0.037475 0.000439 0.027778 0.002505 0.031348 0.000506 0.032075
Mitotic Spindle 0.002816 0.003309 0.001245 0.095084 0.000084 0.005929 0.041893 0.036425 0.001787 0.004753 0.024032 0.068247
None 0.003883 0.022927 0.003727 0.001694 0.005235 0.004329 0.003673 0.009358 0.004393 0.012681 0.003697 0.005200
Nuclear Periphery 0.000596 0.000955 0.002644 0.000942 0.000561 0.000351 0.001800 0.002542 0.000583 0.000622 0.002171 0.001674
Nuclear Periphery Foci 0.000175 0.000578 0.002956 0.001107 0.000233 0.000221 0.001580 0.002214 0.000197 0.000381 0.002184 0.001613
Nucleolus 0.001250 0.005426 0.001626 0.000799 0.001062 0.002404 0.001353 0.004445 0.001179 0.003761 0.001473 0.002467
Nucleus 0.268180* 0.210631* 0.602688* 0.111465 0.241804* 0.084281 0.449330* 0.193647* 0.258241* 0.141024* 0.516715* 0.149064*
Peroxisomes 0.002518 0.020289 0.001061 0.064134 0.002268 0.034327 0.003225 0.013479 0.002424 0.028023 0.002275 0.040959
Vacuole 0.038757 0.043475 0.093816 0.121846 0.024846 0.066957 0.144518 0.164221 0.033515 0.056411 0.122240 0.141233
Vacuole Periphery 0.000603 0.003067 0.000480 0.006110 0.000735 0.003156 0.000374 0.010364 0.000653 0.003116 0.000421 0.008056

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.61 -7.29 -8.50 -8.32 -7.93 -6.35 -7.27 -3.35 -1.77 -1.04 -8.82 -10.15 -8.80 -8.40 -7.95
Bud Neck -3.98 12.37 -0.92 3.21 -12.50 -17.16 1.08 -6.37 11.69 -6.56 -14.61 9.27 -3.90 11.39 -12.27
Bud Site -3.56 -0.83 -18.01 -17.28 -12.09 -11.93 -4.32 -9.49 -1.91 -5.98 -10.99 -2.02 -19.65 -15.34 -14.28
Cell Periphery -0.72 -2.08 0.20 1.37 2.61 -2.28 -4.33 -5.25 -3.20 -2.49 -1.00 -2.78 -2.96 -2.23 -0.02
Cytoplasm 20.37 19.68 27.31 5.46 -4.56 21.17 16.54 15.93 -3.18 -5.80 30.53 25.58 30.66 1.58 -6.96
Cytoplasmic Foci -18.40 17.29 7.63 23.50 -10.38 -16.12 20.34 15.44 34.29 -7.48 -27.14 25.01 15.66 41.26 -12.00
Eisosomes -3.19 1.13 3.34 3.91 1.41 -7.98 -0.23 -1.75 6.14 -1.35 -5.13 1.46 1.81 5.78 0.05
Endoplasmic Reticulum -3.70 -9.80 -9.83 -7.78 6.45 0.86 -8.43 -6.10 -7.67 5.36 -1.42 -12.79 -11.55 -11.22 8.05
Endosome -10.66 -2.33 -7.99 2.98 -5.41 -18.00 -2.30 -10.98 1.95 -8.20 -20.62 -3.02 -13.36 4.15 -9.32
Golgi -8.08 4.79 -8.46 -5.52 -8.73 -11.75 1.35 -5.44 3.83 -5.68 -14.15 2.94 -9.91 -2.42 -10.23
Lipid Particles -8.06 -0.14 4.02 10.31 3.31 -4.90 5.01 -1.17 3.24 -4.61 -9.21 3.23 0.94 9.28 -1.78
Mitochondria -8.85 6.60 -7.99 -0.25 -9.01 -13.68 7.03 -6.69 3.97 -7.05 -16.03 8.19 -10.33 3.02 -11.39
Mitotic Spindle -0.51 1.61 -13.71 -13.62 -14.15 -5.47 -3.63 -8.41 -6.66 0.68 -3.35 -3.37 -15.84 -14.85 -4.54
None -6.21 1.18 3.86 6.96 2.20 0.67 1.64 -1.40 -1.88 -2.60 -5.40 1.84 -0.17 4.99 -1.60
Nuclear Periphery -2.35 -11.30 -6.95 -3.90 7.40 3.56 -9.65 -4.99 -5.56 0.70 -0.47 -14.09 -7.16 -7.06 4.16
Nuclear Periphery Foci -5.62 -5.31 -5.93 -3.60 3.33 0.13 -8.45 -3.26 -3.28 0.78 -4.79 -8.52 -5.46 -4.67 3.17
Nucleolus -5.77 -0.96 1.62 6.40 3.06 -4.42 -0.02 -3.81 -2.09 -3.72 -6.89 -0.47 -3.05 2.90 -2.80
Nucleus 6.43 -15.16 15.80 7.43 20.55 20.83 -8.29 3.73 -15.59 9.89 20.74 -15.07 13.89 -5.47 20.14
Peroxisomes -11.11 3.72 -12.76 -6.87 -13.26 -17.65 0.06 -8.07 10.75 -6.51 -20.56 1.38 -14.23 -0.91 -14.26
Vacuole -1.95 -7.39 -22.98 -21.90 -4.36 -13.49 -11.78 -25.07 -17.81 1.54 -10.46 -13.33 -33.44 -27.50 -0.77
Vacuole Periphery -7.18 1.69 -3.83 -1.07 -4.00 -8.20 1.81 -6.02 -4.04 -6.46 -11.89 2.73 -7.08 -3.68 -7.49
Standard name
Human Ortholog
Description Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate
Localization
Cell Percentages cytoplasm (31%), nucleus (4%), mixed (41%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Cit3

Cit3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cit3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available