Standard name
Human Ortholog
Description (SGD) Mitochondrial inner membrane protein; required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0 0 0.09 0.07 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Cytoplasm 0 0.07 0.09 0.08 0 0.05 0.05 – 0 0 0.05 0 0.09 0.05 0.07 0.31 – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.06 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Endosome 0.07 0 0.11 0.13 0.18 0 0.12 – 0 0.05 0 0 0 0.1 0.1 0 – – 0.06 0.06 0.05 0.07 0.09 0.09
Golgi 0 0 0.09 0 0.06 0 0 – 0 0 0 0 0.09 0.07 0.18 0.25 – – 0 0 0 0 0.06 0.09
Mitochondria 0.78 0.9 0.55 0.69 0.61 0.91 0.7 – 0.89 0.87 0.85 0.9 0.79 0.77 0.69 0.19 – – 0.75 0.76 0.8 0.74 0.64 0.63
Nucleus 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0.07 – 0.05 0 0 0.05 0 0 0 0 – – 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Peroxisomes 0.06 0 0.07 0.15 0 0 0 – 0 0 0 0 0 0.07 0 0.06 – – 0.05 0 0.05 0 0.06 0
SpindlePole 0 0 0.17 0.1 0.07 0 0.06 – 0 0 0 0 0 0 0 0.06 – – 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0.1 0.1 0.09 0.19 0.11 0.17 – 0.08 0.09 0.09 0.08 0.12 0.13 0.1 0.31 – – 0 0.06 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 5 0 0 1 0 – 0 0 0 1 3 3 4 0 – – 3 2 2 1 6 3
Bud 0 4 3 4 2 5 11 – 8 16 14 14 3 5 3 0 – – 1 1 1 1 4 0
Bud Neck 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 2
Bud Site 0 0 0 0 0 3 2 – 3 5 6 3 0 0 1 0 – – – – – – – –
Cell Periphery 4 5 13 13 4 0 4 – 5 5 7 4 4 6 6 0 – – 0 0 0 0 0 2
Cytoplasm 6 12 14 14 10 9 19 – 12 16 19 13 11 15 15 5 – – 0 0 1 0 1 1
Endoplasmic Reticulum 2 10 2 0 4 1 1 – 3 0 2 3 4 6 7 0 – – 0 0 0 0 0 1
Endosome 13 1 16 23 43 1 49 – 4 24 15 12 3 27 21 0 – – 13 10 11 9 24 20
Golgi 3 4 13 2 14 0 2 – 3 2 0 0 11 20 38 4 – – 7 6 3 5 17 19
Mitochondria 154 157 83 125 146 175 278 – 313 406 351 442 92 210 143 3 – – 179 143 190 98 176 138
Nucleus 5 0 2 2 5 8 18 – 4 3 4 6 1 2 1 0 – – 0 0 0 0 0 0
Nuclear Periphery 7 0 0 0 0 4 26 – 17 12 10 23 1 3 1 0 – – 6 0 1 2 2 0
Nucleolus 10 0 0 0 2 8 12 – 13 15 18 19 1 0 1 0 – – 2 0 0 0 4 3
Peroxisomes 12 1 11 28 10 1 10 – 1 8 13 0 2 18 2 1 – – 11 3 12 4 17 9
SpindlePole 4 0 25 18 17 3 22 – 4 17 16 18 1 7 3 1 – – 3 2 1 2 4 2
Vac/Vac Membrane 24 18 15 17 45 21 66 – 27 43 37 37 14 35 21 5 – – 6 10 6 3 10 11
Unique Cell Count 198 175 151 182 238 193 398 352 468 413 492 117 274 208 16 239 188 239 134 275 219
Labelled Cell Count 244 214 202 246 302 240 521 417 572 512 595 151 357 267 19 239 188 239 134 275 219


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 5.1 6.2 6.3 6.7 4.8 6.2 – 5.7 5.9 5.8 5.6 6.1 6.1 6.4 6.8 – – 5.2 5.6 6.1
Std Deviation (1e-4) 12.9 1.0 1.5 2.3 1.2 1.3 1.5 – 1.6 1.4 1.5 1.3 1.5 1.5 1.4 1.8 – – 1.4 1.4 1.8
Intensity Change (Log2) – – – 0.02 0.1 -0.37 -0.01 – -0.12 -0.08 -0.1 -0.14 -0.03 -0.02 0.05 0.13 – – -0.27 -0.15 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Cell Periphery -0.5 -3.3 0 0 – -4.0 0 -3.9 0 -1.7 -3.1 -2.4 0 – –
Cytoplasm -0.5 -2.0 -1.7 -2.0 – -2.7 -2.9 -2.1 -3.6 0 -1.5 -0.7 0 – –
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Endosome 0.6 2.0 0 0.6 – -5.0 -2.4 -3.2 -4.3 -2.5 -0.2 -0.2 0 – –
Golgi -3.3 -1.0 0 0 – 0 0 0 0 0.2 -0.5 2.6 0 – –
Mitochondria 2.6 1.2 0 3.3 – 8.5 8.3 7.5 9.7 4.0 4.6 2.7 -2.8 – –
Nucleus 0 0 0 1.8 – 0 0 0 0 0 0 0 0 – –
Nuclear Periphery 0 0 0 3.2 – 2.7 0 0 2.7 0 0 0 0 – –
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Peroxisomes 2.3 -1.3 0 -2.6 – 0 0 -2.2 0 -2.1 -0.3 -3.2 0 – –
SpindlePole -1.8 -2.9 0 -4.1 – -6.8 -5.5 -5.1 -5.5 -4.3 -5.2 -5.3 0 – –
Vacuole -0.2 2.4 0.3 2.0 – -0.8 -0.3 -0.4 -1.0 0.5 0.9 0.1 0 – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin – – – – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – – – – –
Bud Periphery – – – – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – – – – –
Cytoplasmic Foci – – – – – – – – – – – – – – – – – –
Eisosomes – – – – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – – – – –
Lipid Particles – – – – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – – – – –
None – – – – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – – – – –
Punctate Nuclear – – – – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – – – – –
Vacuole Periphery – – – – – – – – – – – – – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.291 14.9451 33.2697 32.7099 25.2886 50.127 38.6169 43.6286 50.8712 45.4289
Translational Efficiency 2.4127 3.0555 1.6245 2.2621 2.0478 1.4671 1.6974 1.7501 1.6617 1.2574

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization –
Gene Expression ✘
Translational Efficiency ✘


Micrographs

Cell Count

Number of cells analysed for each strain. R1, R2: biological replicates; R1 & R2: both replicates combined.

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
14 8 N/A 2 57 15 N/A N/A 71 23 0 2

Protein Abundance

Mean and standard deviation of cell GFP intensity, and intensity change (log2) of each mutant relative to wild type.

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 305.06 334.62 N/A 323.99 294.01 330.55 N/A N/A 296.19 331.97 — 323.99
Standard Deviation 3.97 3.81 N/A 0.18 7.68 7.60 N/A N/A 8.35 6.82 — 0.18
Intensity Change Log 2 — 0.133431 N/A 0.086856 — 0.169003 N/A N/A — 0.150999 — —

Localization Score (DeepLoc)

Localization score for each compartment. Values highlighted in green denote significant localization scores (marked * in the plot).

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000057 0.000032 N/A 0.000032 0.000061 0.000028 N/A N/A 0.000060 0.000029 N/A 0.000032
Bud Neck 0.000014 0.000019 N/A 0.000004 0.000018 0.000008 N/A N/A 0.000017 0.000011 N/A 0.000004
Bud Site 0.000028 0.000064 N/A 0.000020 0.000036 0.000016 N/A N/A 0.000035 0.000033 N/A 0.000020
Cell Periphery 0.000075 0.000123 N/A 0.000451 0.000117 0.000070 N/A N/A 0.000108 0.000089 N/A 0.000451
Cytoplasm 0.000032 0.000060 N/A 0.000038 0.000048 0.000035 N/A N/A 0.000045 0.000044 N/A 0.000038
Cytoplasmic Foci 0.000013 0.000020 N/A 0.000006 0.000017 0.000012 N/A N/A 0.000016 0.000015 N/A 0.000006
Eisosomes 0.000075 0.000064 N/A 0.000243 0.000141 0.000069 N/A N/A 0.000128 0.000067 N/A 0.000243
Endoplasmic Reticulum 0.000001 0.000001 N/A 0.000001 0.000001 0.000001 N/A N/A 0.000001 0.000001 N/A 0.000001
Endosome 0.000001 0.000001 N/A 0.000000 0.000001 0.000001 N/A N/A 0.000001 0.000001 N/A 0.000000
Golgi 0.000005 0.000007 N/A 0.000003 0.000007 0.000006 N/A N/A 0.000007 0.000006 N/A 0.000003
Lipid Particles 0.000021 0.000038 N/A 0.000008 0.000032 0.000028 N/A N/A 0.000030 0.000032 N/A 0.000008
Mitochondria 0.000012 0.000014 N/A 0.000026 0.000014 0.000011 N/A N/A 0.000014 0.000012 N/A 0.000026
Mitotic Spindle 0.000263 0.000464 N/A 0.000111 0.000294 0.000310 N/A N/A 0.000288 0.000363 N/A 0.000111
None 0.999244* 0.998916* N/A 0.998906* 0.999011* 0.999262* N/A N/A 0.999057* 0.999141* N/A 0.998906*
Nuclear Periphery 0.000000 0.000000 N/A 0.000000 0.000000 0.000000 N/A N/A 0.000000 0.000000 N/A 0.000000
Nuclear Periphery Foci 0.000012 0.000034 N/A 0.000001 0.000022 0.000021 N/A N/A 0.000020 0.000026 N/A 0.000001
Nucleolus 0.000023 0.000028 N/A 0.000002 0.000027 0.000021 N/A N/A 0.000026 0.000024 N/A 0.000002
Nucleus 0.000011 0.000015 N/A 0.000004 0.000013 0.000011 N/A N/A 0.000013 0.000012 N/A 0.000004
Peroxisomes 0.000111 0.000097 N/A 0.000144 0.000138 0.000091 N/A N/A 0.000133 0.000093 N/A 0.000144
Vacuole 0.000001 0.000001 N/A 0.000000 0.000001 0.000000 N/A N/A 0.000001 0.000001 N/A 0.000000
Vacuole Periphery 0.000000 0.000000 N/A 0.000000 0.000000 0.000000 N/A N/A 0.000000 0.000000 N/A 0.000000

Localization Changes (T-score)

Change in localization of each mutant relative to its control (e.g. UBP2_WT: ubp2Δ vs wild type). Values highlighted in yellow (increase) or blue (decrease) denote significant changes (marked * in the plot).

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Bud Neck N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Bud Site N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Cell Periphery N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Cytoplasm N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Cytoplasmic Foci N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Eisosomes N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Endoplasmic Reticulum N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Endosome N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Golgi N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Lipid Particles N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Mitochondria N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Mitotic Spindle N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
None N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Nuclear Periphery N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Nuclear Periphery Foci N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Nucleolus N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Nucleus N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Peroxisomes N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Vacuole N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Vacuole Periphery N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A

No result is found for the queried gene(s). Please try another query.


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Coa1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available