Standard name
Human Ortholog
Description Mitochondrial inner membrane protein; required for proteolytic processing of Cox2p and its assembly into cytochrome c oxidase
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0.1 0.07 0 0 0 0 0 0 0
Cytoplasm 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.07 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Endosome 0.07 0 – 0.05 0.09 0 0.06 – 0 0 0 0 0 0.07 0.06 0 0 0 0.05 0 0 0.05 0.06 0.06
Golgi 0 0 – 0.08 0.17 0 0 – 0 0 0 0 0.15 0.1 0.15 0.36 0.48 0.22 0 0.07 0 0.05 0.07 0.09
Mitochondria 0.8 0.93 – 0.9 0.71 0.92 0.72 – 0.89 0.87 0.91 0.91 0.83 0.81 0.77 0.07 0.07 0 0.83 0.75 0.77 0.76 0.72 0.66
Nucleus 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.08 0 – 0 0 0 0.06 – 0.06 0.05 0.05 0.06 0 0 0 0 0 0.06 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0.06 0.07 – 0.09 0.06 0.05 0.06 0 0 0 0.08 0.07 0.08 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0 0.05 0.06
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.24 0.16 – 0.12 0.22 0.12 0.22 – 0.1 0.1 0.09 0.09 0.17 0.19 0.2 0.28 0.24 0.35 0 0.11 0 0.05 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 2 0 1 0 – 1 0 0 0 1 1 0 2 4 3 0 0 0 5 8 16
Bud 0 2 – 5 2 5 3 – 3 11 7 8 2 6 3 2 5 2 0 0 0 0 1 2
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 4
Bud Site 0 0 – 0 0 4 0 – 1 2 7 5 1 0 0 1 0 0 – – – – – –
Cell Periphery 4 3 – 3 5 0 1 – 0 1 5 3 12 17 7 16 9 3 0 0 0 0 1 0
Cytoplasm 1 0 – 4 8 2 6 – 5 4 4 1 11 18 10 10 9 12 0 0 0 1 2 1
Endoplasmic Reticulum 2 4 – 3 2 1 0 – 2 2 0 3 8 0 1 9 2 4 0 0 0 0 2 2
Endosome 17 0 – 9 21 0 17 – 2 7 2 4 6 34 28 4 3 3 12 3 0 18 29 24
Golgi 5 3 – 15 43 0 8 – 0 4 2 3 48 51 67 58 64 19 8 7 2 16 33 38
Mitochondria 187 94 – 166 176 183 191 – 235 270 306 382 257 402 345 11 9 3 210 77 42 262 335 293
Nucleus 2 2 – 0 1 6 9 – 7 6 9 8 3 1 2 4 2 2 0 0 0 1 1 0
Nuclear Periphery 19 0 – 1 2 6 15 – 16 15 18 23 5 7 0 4 5 5 3 0 1 1 6 3
Nucleolus 9 4 – 0 4 11 18 – 23 18 18 25 9 10 5 13 9 7 0 0 1 1 3 1
Peroxisomes 5 0 – 0 4 0 1 – 1 2 0 3 6 29 21 6 1 3 5 1 2 15 22 25
SpindlePole 5 0 – 3 6 4 7 – 3 2 3 5 2 3 2 3 0 1 0 0 0 1 2 1
Vac/Vac Membrane 56 16 – 23 54 23 59 – 26 32 30 37 52 94 88 44 32 31 7 11 1 15 12 22
Unique Cell Count 233 101 185 247 199 267 264 310 338 418 311 496 449 160 132 88 254 103 55 348 467 443
Labelled Cell Count 312 128 234 328 246 335 325 376 411 510 423 673 579 187 154 98 254 103 55 348 467 443


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 5.9 – 6.4 7.7 5.0 7.4 – 6.3 6.8 6.5 6.8 7.5 8.1 8.3 13.1 13.5 13.2 6.3 6.5 6.7
Std Deviation (1e-4) 1.0 1.0 – 1.1 1.6 1.3 1.8 – 1.4 1.6 1.7 1.6 1.2 1.6 1.5 3.2 2.7 3.4 1.3 1.8 1.4
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.4361 3.1477 2.9012 2.629 2.4667 3.0082 4.5562 4.9726 4.6665 4.0962 4.2448 4.9757 6.961 6.8792 6.5909 6.5053 6.2101 6.6887
Actin 0.0052 0.001 0.0013 0.0009 0.0004 0.0003 0.0051 0.0034 0.0068 0.0003 0.0097 0.0095 0.0127 0.0058 0.01 0.0006 0.0004 0.0017
Bud 0.0008 0.0004 0.0002 0.0001 0.0023 0 0.0002 0.0008 0.0005 0.0001 0.0023 0.0002 0.0011 0.0005 0.0002 0.0007 0.0006 0.0001
Bud Neck 0.0009 0 0.0004 0.0001 0.0001 0.0001 0.0001 0.0006 0.0002 0.0001 0.0003 0.0003 0.0003 0.0002 0.0006 0.0001 0.0002 0.0007
Bud Periphery 0.0013 0.0011 0.0007 0.0003 0.015 0.0001 0.0005 0.0014 0.001 0.0004 0.0063 0.0005 0.006 0.001 0.0007 0.0028 0.0015 0.0004
Bud Site 0.0079 0.0004 0.0014 0.0001 0.0002 0 0.0005 0.0043 0.002 0.0001 0.0013 0.0003 0.0011 0.0023 0.0011 0.0003 0.0007 0.0001
Cell Periphery 0.0007 0.0004 0.0005 0.0003 0.0003 0.0001 0.0003 0.0007 0.0004 0.0001 0.0002 0.0001 0.0005 0.0004 0.0041 0.0003 0.0005 0.0003
Cytoplasm 0.0003 0.0002 0.0002 0 0.0001 0.0001 0.004 0.0003 0.0003 0 0.0005 0.0001 0.0002 0.0005 0.0003 0.0001 0.0002 0.0001
Cytoplasmic Foci 0.0063 0.0016 0.0047 0.0017 0.0046 0.0013 0.0126 0.0083 0.0029 0.0032 0.0186 0.0033 0.0042 0.0074 0.0037 0.0012 0.0039 0.001
Eisosomes 0.0004 0.0003 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0002 0.0001 0.0002 0.0001 0.0004 0.0001 0.0004 0.0001 0.0001 0.0002
Endoplasmic Reticulum 0.0004 0.0002 0.0018 0 0.0001 0.0002 0.0007 0.0002 0.0005 0.0001 0.0005 0.0003 0.0004 0.0003 0.0002 0.0001 0.0001 0.0005
Endosome 0.0223 0.0143 0.0106 0.0073 0.0146 0.0117 0.0309 0.025 0.0117 0.0132 0.0245 0.0158 0.0314 0.0389 0.022 0.0103 0.0458 0.0077
Golgi 0.0236 0.0116 0.014 0.0116 0.0256 0.0118 0.0213 0.0247 0.0139 0.0205 0.0163 0.0208 0.0258 0.0486 0.0175 0.0036 0.027 0.0108
Lipid Particles 0.0114 0.0035 0.0071 0.0017 0.0077 0.0028 0.0213 0.0056 0.0054 0.0085 0.0204 0.0105 0.0151 0.0131 0.0034 0.0009 0.0029 0.0074
Mitochondria 0.8868 0.9332 0.9302 0.9708 0.9199 0.9616 0.8514 0.8907 0.9345 0.9394 0.8499 0.9101 0.8581 0.852 0.9018 0.9633 0.881 0.9536
None 0.0002 0.0001 0.0001 0 0.0001 0 0.0104 0.0001 0.0004 0 0.0007 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001
Nuclear Periphery 0.0005 0.0001 0.0015 0 0 0.0002 0.0042 0.0001 0.0011 0.0001 0.0014 0.0024 0.0019 0.0003 0.0002 0.0001 0.0001 0.0042
Nucleolus 0.0003 0.0001 0.0002 0 0.0002 0 0.0006 0.0007 0.0003 0.0002 0.0014 0.0002 0.0075 0.0003 0.0003 0.0002 0.0003 0.0003
Nucleus 0.0003 0.0001 0.0002 0 0.0001 0 0.0019 0.0003 0.0002 0.0001 0.0009 0.0002 0.0006 0.0002 0.0003 0.0002 0.0002 0.0002
Peroxisomes 0.0131 0.019 0.0069 0.0021 0.0037 0.0052 0.0082 0.017 0.0055 0.0077 0.0359 0.0071 0.0069 0.0091 0.005 0.0032 0.004 0.002
Punctate Nuclear 0.0007 0 0.0001 0 0 0 0.0051 0.0001 0.0001 0 0.0041 0.0004 0.0001 0 0 0 0 0.0001
Vacuole 0.0087 0.0039 0.0073 0.0009 0.002 0.001 0.0074 0.0093 0.0047 0.0016 0.0018 0.0052 0.0117 0.0068 0.017 0.006 0.018 0.0022
Vacuole Periphery 0.0079 0.0085 0.0106 0.0018 0.0028 0.0033 0.013 0.0064 0.0074 0.0041 0.0026 0.0123 0.014 0.0118 0.011 0.0059 0.0123 0.0064

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.3181 11.1725 35.5308 22.2163 10.4171 41.0425 25.0138 28.3192 39.2302 19.2694
Translational Efficiency 0.993 2.4519 0.6604 1.8771 1.9894 0.6509 1.0422 1.2452 0.9605 0.9952

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
628 1191 2721 1282 162 1952 2555 1679 790 3143 5276 2961

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 679.58 916.00 943.34 841.63 769.77 869.64 852.93 959.73 698.07 887.21 899.56 908.60
Standard Deviation 87.81 135.58 134.07 132.81 100.32 124.90 114.41 130.91 97.57 131.00 132.85 144.15
Intensity Change Log 2 — 0.430704 0.473134 0.308543 — 0.175991 0.148000 0.318201 — 0.301035 0.309598 0.313681

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000415 0.001444 0.003898 0.006859 0.001485 0.000438 0.000859 0.005197 0.000634 0.000819 0.002426 0.005917
Bud Neck 0.002886 0.001591 0.001370 0.004293 0.014568 0.004659 0.020036 0.001933 0.005282 0.003496 0.010409 0.002955
Bud Site 0.006330 0.008000 0.030686 0.049703 0.018412 0.006451 0.031725 0.037252 0.008808 0.007038 0.031189 0.042643
Cell Periphery 0.000897 0.000456 0.000316 0.001026 0.001489 0.000607 0.000677 0.000808 0.001019 0.000550 0.000491 0.000902
Cytoplasm 0.001096 0.001004 0.006862 0.007422 0.002283 0.002125 0.013954 0.006593 0.001339 0.001701 0.010296 0.006952
Cytoplasmic Foci 0.017874 0.032603 0.036480 0.039520 0.029028 0.018186 0.037349 0.034447 0.020161 0.023649 0.036901 0.036643
Eisosomes 0.000053 0.000055 0.000067 0.000113 0.000074 0.000051 0.000085 0.000089 0.000057 0.000053 0.000076 0.000099
Endoplasmic Reticulum 0.001445 0.000734 0.001085 0.001678 0.000863 0.000550 0.000795 0.000873 0.001325 0.000620 0.000944 0.001222
Endosome 0.033805 0.052920 0.046804 0.071720 0.070747 0.060152 0.100571 0.050426 0.041380 0.057412 0.072841 0.059645
Golgi 0.053207 0.069264 0.135006* 0.114196* 0.046094 0.048539 0.087046 0.137957* 0.051748 0.056392 0.111781* 0.127669*
Lipid Particles 0.003114 0.002851 0.000429 0.000514 0.001141 0.004803 0.004860 0.000179 0.002709 0.004063 0.002575 0.000324
Mitochondria 0.834630* 0.765167* 0.603229* 0.573318* 0.710953* 0.789295* 0.540100* 0.629476* 0.809269* 0.780152* 0.572658* 0.605162*
Mitotic Spindle 0.005519 0.008629 0.041488 0.033089 0.008258 0.004486 0.030569 0.026992 0.006081 0.006056 0.036200 0.029632
None 0.000302 0.000274 0.000394 0.000419 0.000281 0.000839 0.001005 0.000403 0.000298 0.000625 0.000690 0.000410
Nuclear Periphery 0.000119 0.000120 0.000215 0.000195 0.000465 0.000215 0.000299 0.000073 0.000190 0.000179 0.000256 0.000126
Nuclear Periphery Foci 0.000143 0.000233 0.000499 0.000385 0.000404 0.000327 0.000379 0.000151 0.000197 0.000291 0.000441 0.000253
Nucleolus 0.000313 0.000194 0.000082 0.000141 0.000271 0.001162 0.002032 0.000080 0.000304 0.000795 0.001026 0.000107
Nucleus 0.000772 0.000957 0.000529 0.000517 0.000473 0.001192 0.004544 0.000156 0.000711 0.001103 0.002473 0.000313
Peroxisomes 0.016937 0.016204 0.008148 0.019387 0.040988 0.012101 0.017450 0.011056 0.021869 0.013656 0.012653 0.014663
Vacuole 0.004658 0.015462 0.038495 0.040270 0.015270 0.022903 0.063739 0.023020 0.006834 0.020083 0.050720 0.030489
Vacuole Periphery 0.015486 0.021838 0.043917 0.035234 0.036453 0.020918 0.041926 0.032839 0.019786 0.021267 0.042953 0.033876

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.17 -7.96 -5.65 -4.65 -2.41 1.39 0.81 -3.25 -5.43 -4.93 -0.92 -6.33 -7.39 -7.21 -4.73
Bud Neck 1.32 1.54 -1.40 -4.68 -5.21 2.10 -1.11 2.71 3.32 10.66 1.35 -3.49 1.79 0.84 8.47
Bud Site -0.96 -11.81 -12.00 -11.53 -5.03 2.55 -2.74 -3.64 -12.34 -1.90 1.16 -12.48 -13.98 -16.90 -4.94
Cell Periphery 1.37 1.83 -0.36 -3.61 -4.77 1.69 1.56 1.30 -1.65 -1.05 1.69 1.91 0.41 -3.58 -4.45
Cytoplasm 0.43 -9.57 -7.66 -7.93 -0.57 0.19 -9.08 -6.35 -7.27 5.87 -1.16 -13.29 -11.91 -10.41 4.37
Cytoplasmic Foci -5.42 -8.85 -8.58 -2.46 -1.35 2.52 -2.01 -1.26 -8.03 1.50 -1.85 -9.10 -8.32 -7.82 0.26
Eisosomes -0.48 -2.84 -7.60 -8.24 -6.71 2.37 -1.11 -1.40 -7.73 -0.63 1.02 -4.10 -7.53 -11.34 -5.50
Endoplasmic Reticulum 0.84 0.42 -0.29 -2.31 -1.90 1.34 0.24 -0.04 -1.67 -0.34 1.09 0.57 0.15 -3.00 -1.50
Endosome -4.53 -3.67 -8.76 -4.73 -7.69 1.08 -3.01 2.11 2.87 14.26 -4.19 -8.61 -5.00 -0.86 5.60
Golgi -2.71 -13.86 -9.18 -7.39 3.48 -0.36 -5.94 -11.72 -16.72 -9.23 -1.05 -13.17 -14.71 -17.85 -3.86
Lipid Particles 0.22 2.56 2.49 3.02 -0.36 -4.01 -4.90 3.78 5.26 6.48 -1.31 0.12 2.90 6.02 6.05
Mitochondria 5.20 19.45 18.83 14.78 2.60 -2.95 6.47 3.03 15.25 -8.40 2.63 21.98 17.72 21.44 -4.16
Mitotic Spindle -1.53 -13.99 -9.24 -8.50 2.51 2.29 -9.02 -7.83 -12.00 1.38 0.05 -15.96 -12.01 -14.52 3.16
None 0.49 -0.60 -0.74 -2.43 -0.41 -1.91 -5.47 -2.79 1.49 4.55 -1.54 -3.53 -1.00 1.18 4.03
Nuclear Periphery -0.02 -1.36 -1.09 -1.82 0.37 0.69 0.45 1.09 2.77 4.07 0.12 -0.72 0.71 1.44 3.47
Nuclear Periphery Foci -1.22 -1.65 -2.22 -1.64 0.51 0.43 0.02 1.45 2.91 3.19 -1.35 -1.99 -0.75 0.67 1.64
Nucleolus 0.59 0.93 0.68 0.30 -1.90 -3.11 -4.21 1.95 3.97 4.74 -1.36 -2.08 0.95 3.92 4.54
Nucleus 0.03 0.44 0.48 0.54 0.13 -2.06 -5.92 2.46 3.15 6.46 -0.29 -2.14 0.79 2.23 6.13
Peroxisomes 0.23 3.09 -0.79 -1.31 -5.44 3.21 2.60 3.32 0.61 3.59 2.74 3.11 2.34 -0.72 -1.47
Vacuole -7.77 -20.06 -14.56 -9.39 -0.66 -2.49 -13.86 -2.58 -0.12 15.51 -10.44 -28.23 -15.70 -6.44 10.67
Vacuole Periphery -2.62 -11.95 -7.65 -4.99 3.32 2.12 -0.73 0.48 -5.25 3.62 -0.66 -9.99 -5.79 -7.33 4.99
Standard name
Human Ortholog
Description Mitochondrial inner membrane protein; required for proteolytic processing of Cox2p and its assembly into cytochrome c oxidase
Localization
Cell Percentages mitochondrion (93%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Cox20

Cox20


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cox20-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available