Standard name
Human Ortholog
Description Mitochondrial inner membrane protein; required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0.08 0.07 0 0.05 – 0.05 0.07 0 0.05 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.8 0.76 0.64 0.61 0.52 0.41 – 0.3 0.27 0.23 0.21 0.24 0.9 0.92 0.91 – – – 0.39 0.29 0.15 0.26 0.29 0.33
Endoplasmic Reticulum 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0.21 0 – 0 0 0 0 0 0 0 0 – – – 0.05 0.05 0 0 0.05 0.06
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.19 0.16 0.43 0.54 0.27 0.65 – 0.67 0.8 0.8 0.81 0.8 0 0 0 – – – 0.35 0.46 0.56 0.47 0.41 0.32
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.07 0 – 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.07 0 – 0.1 0.07 0.08 0.05 0.09 0 0 0 – – – 0 0 0.05 0 0.05 0
Vac/Vac Membrane 0.07 0.07 0 0 0.06 0.08 – 0 0 0 0 0 0 0 0.05 – – – 0.07 0.06 0 0.09 0.07 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 0 0 0 0 – 0 0 0 0 0 0 1 0 – – – 5 4 6 1 7 9
Bud 0 0 14 15 4 11 – 20 22 13 15 11 1 0 2 – – – 2 0 3 0 6 9
Bud Neck 0 1 4 0 0 0 – 2 1 1 0 0 0 0 0 – – – 1 2 3 1 5 4
Bud Site 0 0 0 0 1 1 – 3 2 4 0 8 0 0 0 – – – – – – – – –
Cell Periphery 0 2 2 5 0 1 – 4 2 1 1 1 0 0 1 – – – 1 0 1 0 0 2
Cytoplasm 138 99 118 132 146 98 – 118 82 69 56 65 193 307 232 – – – 85 51 30 31 63 94
Endoplasmic Reticulum 1 7 1 2 0 0 – 0 0 0 0 0 1 2 1 – – – 1 2 5 1 1 3
Endosome 0 5 1 4 58 6 – 13 2 3 6 1 9 9 11 – – – 10 8 6 1 10 16
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 1 1 – – – 1 0 0 0 3 1
Mitochondria 32 21 80 118 76 155 – 267 245 246 221 221 9 13 7 – – – 77 80 111 57 89 91
Nucleus 0 1 0 0 3 2 – 5 3 2 2 3 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 1 0 0 0 – 1 1 0 0 2 0 0 0 – – – 1 0 0 1 1 0
Nucleolus 1 0 0 0 1 0 – 3 1 1 1 2 2 1 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 2 4 21 1 – 10 9 4 11 5 0 1 2 – – – 0 0 2 0 0 0
SpindlePole 1 0 5 6 19 6 – 39 20 23 14 24 0 2 1 – – – 5 4 9 4 10 12
Vac/Vac Membrane 12 9 5 1 16 19 – 12 7 12 4 2 9 12 12 – – – 15 11 7 11 14 28
Unique Cell Count 172 130 184 218 280 238 398 306 306 273 276 214 334 254 220 176 200 122 221 281
Labelled Cell Count 186 146 233 287 345 300 497 397 379 331 345 224 349 270 220 176 200 122 221 281


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 4.2 3.7 3.8 4.1 3.7 – 3.4 3.0 3.2 3.3 3.0 4.6 4.8 4.8 – – – 4.2 4.8 4.7
Std Deviation (1e-4) 0.6 1.0 2.2 3.1 1.7 3.4 – 2.3 1.7 1.3 2.0 1.1 1.2 1.7 1.7 – – – 1.2 1.7 1.6
Intensity Change (Log2) – – – 0.07 0.17 0.03 – -0.11 -0.28 -0.21 -0.16 -0.3 0.33 0.38 0.38 – – – 0.19 0.39 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.6377 -0.2512 -0.3123 -0.5617 -0.6648 -0.1903 -0.3423 -0.3662 -0.5204 0.3582 -0.4023 -0.5557 -0.0495 0.597 0.1804 -0.0733 -0.2149 0.4069
Actin 0.0034 0.0008 0.0046 0.0158 0.0016 0.0032 0.0415 0.0018 0.0185 0.0302 0.0414 0.0074 0.003 0.0062 0.0052 0.0048 0.0026 0.0026
Bud 0.0007 0.0006 0.0015 0.0011 0.0036 0.0007 0.0026 0.002 0.0034 0.0088 0.003 0.0012 0.003 0.0089 0.0023 0.0016 0.002 0.0019
Bud Neck 0.0009 0.0023 0.0092 0.0044 0.0016 0.0082 0.0046 0.0007 0.005 0.0306 0.0013 0.0093 0.001 0.0011 0.003 0.001 0.0004 0.0107
Bud Periphery 0.0015 0.0014 0.0039 0.0036 0.0047 0.0027 0.0066 0.0033 0.0089 0.0349 0.0058 0.005 0.0028 0.0058 0.0043 0.0052 0.0072 0.0029
Bud Site 0.0032 0.0045 0.0127 0.0022 0.0023 0.0012 0.0119 0.0143 0.0123 0.0132 0.0026 0.0012 0.0024 0.0266 0.0083 0.0012 0.0008 0.001
Cell Periphery 0.0021 0.0007 0.0012 0.001 0.0021 0.0006 0.0011 0.0009 0.0018 0.0037 0.0006 0.0008 0.0009 0.0012 0.0014 0.0009 0.0011 0.0009
Cytoplasm 0.0779 0.1029 0.1266 0.0476 0.1736 0.1567 0.0783 0.1478 0.1199 0.1007 0.2187 0.1565 0.1004 0.1428 0.1526 0.1226 0.1682 0.1827
Cytoplasmic Foci 0.1207 0.1313 0.139 0.0788 0.1537 0.1341 0.1208 0.1103 0.1099 0.1148 0.1539 0.1479 0.1565 0.1093 0.101 0.0812 0.1941 0.1202
Eisosomes 0.0008 0.0004 0.0017 0.0015 0.0005 0.0003 0.001 0.0005 0.0032 0.0016 0.0003 0.0004 0.0008 0.0008 0.0009 0.0005 0.0007 0.0003
Endoplasmic Reticulum 0.0051 0.003 0.0072 0.0038 0.0093 0.0049 0.0078 0.0036 0.0059 0.0069 0.0045 0.0039 0.0094 0.0059 0.0091 0.014 0.0052 0.0043
Endosome 0.0859 0.0862 0.0823 0.0258 0.1158 0.0829 0.1129 0.0767 0.0733 0.0656 0.1328 0.1029 0.0947 0.077 0.0781 0.1131 0.1294 0.0692
Golgi 0.0114 0.0141 0.0144 0.0114 0.0125 0.0105 0.0251 0.013 0.0143 0.0221 0.0379 0.0246 0.0178 0.0149 0.0121 0.0285 0.0176 0.011
Lipid Particles 0.0392 0.0078 0.0259 0.0061 0.0127 0.018 0.0363 0.007 0.0144 0.0358 0.0336 0.0165 0.0173 0.0079 0.0094 0.0157 0.0063 0.0084
Mitochondria 0.08 0.0637 0.0986 0.1423 0.0617 0.0528 0.1209 0.0531 0.0837 0.1054 0.0354 0.1136 0.0521 0.0514 0.0637 0.2293 0.0756 0.0699
None 0.534 0.5504 0.4332 0.5924 0.4176 0.4886 0.3454 0.5273 0.4691 0.33 0.268 0.3588 0.4846 0.5112 0.505 0.3026 0.3516 0.4665
Nuclear Periphery 0.002 0.0014 0.0013 0.0022 0.0012 0.0012 0.0147 0.0016 0.004 0.0063 0.0036 0.0018 0.0028 0.0009 0.0021 0.0042 0.0017 0.0016
Nucleolus 0.0013 0.001 0.0009 0.0013 0.0007 0.0008 0.002 0.0024 0.002 0.0058 0.0011 0.0007 0.0014 0.0011 0.0011 0.0009 0.0005 0.0006
Nucleus 0.0027 0.0022 0.0015 0.0027 0.0014 0.0023 0.0048 0.004 0.0041 0.0098 0.0023 0.0023 0.0103 0.0022 0.0022 0.0018 0.0015 0.007
Peroxisomes 0.0152 0.0158 0.0235 0.0462 0.0098 0.0172 0.0379 0.0071 0.0248 0.0421 0.0259 0.0295 0.022 0.013 0.0215 0.0442 0.0214 0.0255
Punctate Nuclear 0.0039 0.0029 0.0022 0.0033 0.0015 0.0054 0.0057 0.0034 0.0063 0.0072 0.0127 0.0049 0.0027 0.0016 0.0036 0.0009 0.0011 0.0055
Vacuole 0.0069 0.0051 0.0067 0.0044 0.0099 0.0057 0.0138 0.0167 0.012 0.0186 0.0113 0.0077 0.0118 0.0083 0.0096 0.018 0.0079 0.0052
Vacuole Periphery 0.0013 0.0015 0.0022 0.002 0.0023 0.0021 0.0042 0.0023 0.003 0.0059 0.0033 0.0032 0.0023 0.0018 0.0033 0.0079 0.0032 0.0022

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.2715 20.5495 52.281 35.5515 31.9333 52.514 48.2337 53.0888 70.587 39.1083
Translational Efficiency 2.7809 2.9315 1.3094 2.8907 2.281 1.3169 1.5476 2.0536 1.2638 1.7017

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1643 1601 199 1011 1675 2028 350 1725 3318 3629 549 2736

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 589.26 690.30 912.77 803.72 647.78 720.34 851.00 825.57 618.80 707.09 873.39 817.50
Standard Deviation 71.94 97.31 88.97 107.73 71.05 88.81 99.39 106.24 77.25 93.85 100.24 107.31
Intensity Change Log 2 — 0.228319 0.631347 0.447789 — 0.153174 0.393655 0.349887 — 0.189458 0.511770 0.397351

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000382 0.000929 0.000343 0.000781 0.000301 0.000899 0.000365 0.001945 0.000341 0.000912 0.000357 0.001515
Bud Neck 0.056479 0.059000 0.001833 0.013470 0.042397 0.074538 0.006823 0.019827 0.049370 0.067683 0.005014 0.017478
Bud Site 0.007727 0.034262 0.002968 0.015884 0.005554 0.019330 0.007069 0.027424 0.006630 0.025918 0.005582 0.023160
Cell Periphery 0.000208 0.000177 0.000253 0.000230 0.000150 0.000260 0.000163 0.000203 0.000179 0.000223 0.000196 0.000213
Cytoplasm 0.207496* 0.167823* 0.203733* 0.293011* 0.212782* 0.156950* 0.224225* 0.339856* 0.210164* 0.161746* 0.216797* 0.322546*
Cytoplasmic Foci 0.357701* 0.281603* 0.038016 0.067713 0.403316* 0.319779* 0.020385 0.044894 0.380728* 0.302937* 0.026776 0.053326
Eisosomes 0.000422 0.000159 0.000125 0.000106 0.000333 0.000250 0.000058 0.000088 0.000377 0.000210 0.000083 0.000094
Endoplasmic Reticulum 0.001184 0.000959 0.004997 0.006052 0.001354 0.000796 0.007263 0.005322 0.001270 0.000868 0.006442 0.005592
Endosome 0.034141 0.061112 0.008668 0.014022 0.035000 0.054447 0.003235 0.006882 0.034575 0.057388 0.005204 0.009521
Golgi 0.015996 0.030094 0.000196 0.002526 0.012172 0.023724 0.000100 0.002412 0.014065 0.026534 0.000135 0.002454
Lipid Particles 0.043051 0.015782 0.006250 0.006142 0.036367 0.019737 0.002797 0.003351 0.039676 0.017992 0.004049 0.004382
Mitochondria 0.090102 0.107525* 0.000459 0.010744 0.058801 0.118518* 0.000910 0.005110 0.074301 0.113668* 0.000746 0.007192
Mitotic Spindle 0.001328 0.026932 0.000606 0.020450 0.003193 0.009646 0.000918 0.015473 0.002270 0.017272 0.000804 0.017312
None 0.003677 0.003843 0.003943 0.011193 0.001461 0.002461 0.003366 0.003240 0.002558 0.003071 0.003575 0.006179
Nuclear Periphery 0.000758 0.000755 0.002170 0.003511 0.000974 0.000549 0.002088 0.002398 0.000867 0.000640 0.002118 0.002809
Nuclear Periphery Foci 0.000257 0.000551 0.002014 0.003017 0.000323 0.000300 0.001555 0.001372 0.000291 0.000410 0.001721 0.001980
Nucleolus 0.002618 0.003830 0.002233 0.003505 0.002165 0.002589 0.001270 0.001263 0.002389 0.003137 0.001619 0.002092
Nucleus 0.091852 0.064876 0.636462* 0.380232* 0.089419 0.062982 0.653426* 0.405812* 0.090623 0.063818 0.647277* 0.396360*
Peroxisomes 0.042283 0.065928 0.001266 0.008478 0.043773 0.060412 0.000941 0.008972 0.043035 0.062845 0.001059 0.008790
Vacuole 0.038299 0.066466 0.082708 0.135252 0.046798 0.066087 0.062661 0.103430 0.042589 0.066254 0.069928 0.115189
Vacuole Periphery 0.004040 0.007393 0.000755 0.003681 0.003369 0.005746 0.000383 0.000726 0.003701 0.006473 0.000518 0.001818

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.53 -0.81 -3.94 0.63 -3.32 -2.39 -2.83 -5.00 -2.13 -4.34 -3.37 -2.48 -5.64 -1.83 -4.93
Bud Neck -0.50 19.38 14.81 14.58 -8.64 -8.42 13.33 9.33 16.88 -5.92 -6.42 21.94 16.64 21.89 -8.34
Bud Site -11.40 3.71 -2.69 7.27 -4.47 -11.31 -0.68 -7.63 -0.50 -4.77 -15.59 0.64 -7.76 4.48 -6.11
Cell Periphery 1.76 -2.80 0.62 -0.95 2.93 -1.60 -0.99 -1.71 1.27 -0.10 -1.10 -1.87 -0.18 1.04 1.60
Cytoplasm 4.82 0.89 -6.22 -10.27 -4.29 7.89 0.73 -9.78 -17.76 -6.90 9.06 1.05 -11.80 -20.22 -8.08
Cytoplasmic Foci 9.51 43.88 44.84 33.55 -4.46 10.95 58.88 61.39 49.91 -5.43 14.03 73.08 75.90 60.03 -7.03
Eisosomes 10.55 10.49 12.44 5.83 2.28 3.31 11.06 10.35 14.43 -3.22 9.39 15.88 16.31 15.63 -0.67
Endoplasmic Reticulum 1.36 -8.76 -14.21 -15.11 -1.66 2.60 -8.04 -14.20 -17.92 2.32 2.97 -10.44 -20.20 -23.32 1.55
Endosome -8.61 11.30 11.08 17.32 -1.35 -7.14 17.69 16.78 22.70 -2.41 -11.14 21.40 20.55 28.68 -2.60
Golgi -7.01 14.89 12.18 16.37 -2.60 -7.99 16.10 11.77 16.80 -2.49 -10.30 21.49 16.73 23.36 -3.46
Lipid Particles 10.82 15.87 16.63 7.08 0.80 7.69 17.94 17.98 13.23 -1.45 13.06 24.12 24.59 14.88 -0.52
Mitochondria -2.42 20.82 18.00 19.63 -3.65 -10.54 16.94 15.94 25.35 -4.22 -8.97 26.68 24.41 32.25 -5.33
Mitotic Spindle -10.22 1.09 -6.21 2.14 -6.36 -4.08 0.75 -6.03 -2.84 -6.49 -10.68 0.73 -8.90 0.04 -9.01
None -0.05 0.20 -2.76 -2.60 -2.68 -2.03 -3.92 -5.91 -0.79 0.54 -0.89 -1.37 -3.49 -2.50 -2.28
Nuclear Periphery -0.00 -8.40 -10.94 -9.56 -5.05 2.92 -7.06 -12.28 -16.70 -4.36 1.78 -10.85 -16.95 -17.13 -6.64
Nuclear Periphery Foci -2.79 -6.08 -10.34 -9.08 -2.50 0.40 -5.36 -8.10 -8.16 -0.46 -2.04 -7.94 -13.00 -11.97 -1.94
Nucleolus -2.37 1.22 -0.97 1.70 -2.30 -0.45 2.64 0.93 1.58 -1.80 -1.94 3.12 0.50 2.50 -2.70
Nucleus 5.14 -24.83 -28.91 -31.50 10.70 5.89 -36.05 -41.55 -46.23 13.91 7.87 -43.71 -50.64 -55.68 17.57
Peroxisomes -6.46 19.49 15.23 18.95 -5.51 -4.88 19.35 14.74 19.11 -6.33 -7.97 27.48 20.93 26.73 -8.24
Vacuole -8.84 -6.56 -21.71 -16.53 -6.67 -6.01 -5.46 -21.25 -16.68 -6.95 -10.50 -8.45 -30.49 -23.29 -9.58
Vacuole Periphery -4.40 8.45 2.16 4.92 -1.91 -6.14 12.67 11.96 16.32 -1.37 -6.94 15.10 7.48 11.77 -2.17
Standard name
Human Ortholog
Description Mitochondrial inner membrane protein; required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA
Localization
Cell Percentages mitochondrion (28%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Qri5

Qri5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Qri5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available