| Standard name | |
|---|---|
| Human Ortholog | |
| Description (SGD) | Subunit Va of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Ap is predominantly expressed during aerobic growth while its isoform Vb (Cox5Bp) is expressed during anaerobic growth; COX5A has a paralog, COX5B, that arose from the whole genome duplication |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 0 | 2 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 6 | 0 | – | 0 | – |
| Bud | 1 | 3 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | 0 | – |
| Bud Neck | 0 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | 0 | – |
| Bud Site | 0 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | 3 | 9 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | 0 | – |
| Cytoplasm | 0 | 1 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | 0 | – |
| Endoplasmic Reticulum | 1 | 1 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 1 | 0 | 0 | – | 0 | – |
| Endosome | 3 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 3 | 8 | 0 | – | 0 | – |
| Golgi | 5 | 15 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 4 | 19 | 0 | – | 0 | – |
| Mitochondria | 133 | 206 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 129 | 178 | 0 | – | 0 | – |
| Nucleus | 0 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | 0 | – |
| Nuclear Periphery | 10 | 10 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 2 | 0 | – | 0 | – |
| Nucleolus | 14 | 3 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | 0 | – |
| Peroxisomes | 1 | 4 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 6 | 6 | 0 | – | 0 | – |
| SpindlePole | 1 | 1 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | 0 | – |
| Vac/Vac Membrane | 24 | 42 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 1 | 12 | 0 | – | 0 | – |
| Unique Cell Count | 149 | 226 | 148 | 238 | 1 | 1 | ||||||||||||||||||
| Labelled Cell Count | 196 | 297 | 148 | 238 | 1 | 1 | ||||||||||||||||||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 9.7 | 10.4 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Std Deviation (1e-4) | 1.7 | 1.9 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|
External localization resources
Images
Protein Concentration and Protein Localization Data
| R1 | R2 | R3 | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasmic Foci | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Eisosomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Lipid Particles | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| None | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Punctate Nuclear | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Sequencing Data
| R1 | R2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Gene Expression | 50.1547 | 51.2403 | 44.3596 | 67.804 | 63.4795 | 26.257 | 48.571 | 57.5509 | 77.1942 | 53.5835 |
| Translational Efficiency | 2.1673 | 2.0935 | 2.8006 | 2.227 | 2.3066 | 5.1658 | 2.4781 | 2.2988 | 1.9619 | 2.1423 |
Hit Data
| Dataset | Hit |
|---|---|
| Protein Concentration | – |
| Protein Localization | – |
| Gene Expression | ✘ |
| Translational Efficiency | ✘ |
Micrographs
Cell Count
Number of cells analysed for each strain. R1, R2: biological replicates; R1 & R2: both replicates combined.
| R1 | R2 | R1 & R2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
| 57 | 57 | N/A | N/A | 90 | 32 | N/A | N/A | 147 | 89 | 0 | 0 |
Protein Abundance
Mean and standard deviation of cell GFP intensity, and intensity change (log2) of each mutant relative to wild type.
| R1 | R2 | R1 & R2 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
| Mean | 291.81 | 312.19 | N/A | N/A | 291.53 | 316.23 | N/A | N/A | 291.64 | 313.64 | — | — |
| Standard Deviation | 9.93 | 4.67 | N/A | N/A | 5.88 | 12.35 | N/A | N/A | 7.71 | 8.52 | — | — |
| Intensity Change Log 2 | — | 0.097395 | N/A | N/A | — | 0.117330 | N/A | N/A | — | 0.107392 | — | — |
Localization Score (DeepLoc)
Localization score for each compartment. Values highlighted in green denote significant localization scores (marked * in the plot).
| R1 | R2 | R1 & R2 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
| Actin | 0.000049 | 0.000028 | N/A | N/A | 0.000063 | 0.000026 | N/A | N/A | 0.000058 | 0.000027 | N/A | N/A |
| Bud Neck | 0.000008 | 0.000013 | N/A | N/A | 0.000020 | 0.000017 | N/A | N/A | 0.000016 | 0.000015 | N/A | N/A |
| Bud Site | 0.000016 | 0.000025 | N/A | N/A | 0.000054 | 0.000029 | N/A | N/A | 0.000039 | 0.000027 | N/A | N/A |
| Cell Periphery | 0.000070 | 0.000071 | N/A | N/A | 0.000139 | 0.000063 | N/A | N/A | 0.000112 | 0.000068 | N/A | N/A |
| Cytoplasm | 0.000028 | 0.000048 | N/A | N/A | 0.000116 | 0.000057 | N/A | N/A | 0.000082 | 0.000051 | N/A | N/A |
| Cytoplasmic Foci | 0.000012 | 0.000020 | N/A | N/A | 0.000023 | 0.000027 | N/A | N/A | 0.000019 | 0.000023 | N/A | N/A |
| Eisosomes | 0.000117 | 0.000059 | N/A | N/A | 0.000157 | 0.000041 | N/A | N/A | 0.000142 | 0.000053 | N/A | N/A |
| Endoplasmic Reticulum | 0.000001 | 0.000001 | N/A | N/A | 0.000001 | 0.000001 | N/A | N/A | 0.000001 | 0.000001 | N/A | N/A |
| Endosome | 0.000001 | 0.000002 | N/A | N/A | 0.000002 | 0.000003 | N/A | N/A | 0.000002 | 0.000002 | N/A | N/A |
| Golgi | 0.000005 | 0.000006 | N/A | N/A | 0.000007 | 0.000006 | N/A | N/A | 0.000006 | 0.000006 | N/A | N/A |
| Lipid Particles | 0.000026 | 0.000028 | N/A | N/A | 0.000033 | 0.000055 | N/A | N/A | 0.000030 | 0.000038 | N/A | N/A |
| Mitochondria | 0.000010 | 0.000011 | N/A | N/A | 0.000017 | 0.000010 | N/A | N/A | 0.000015 | 0.000011 | N/A | N/A |
| Mitotic Spindle | 0.000231 | 0.000333 | N/A | N/A | 0.000264 | 0.000587 | N/A | N/A | 0.000251 | 0.000424 | N/A | N/A |
| None | 0.999288* | 0.999125* | N/A | N/A | 0.998882* | 0.998743* | N/A | N/A | 0.999039* | 0.998988* | N/A | N/A |
| Nuclear Periphery | 0.000000 | 0.000000 | N/A | N/A | 0.000000 | 0.000001 | N/A | N/A | 0.000000 | 0.000000 | N/A | N/A |
| Nuclear Periphery Foci | 0.000016 | 0.000083 | N/A | N/A | 0.000027 | 0.000168 | N/A | N/A | 0.000023 | 0.000114 | N/A | N/A |
| Nucleolus | 0.000019 | 0.000052 | N/A | N/A | 0.000043 | 0.000070 | N/A | N/A | 0.000034 | 0.000059 | N/A | N/A |
| Nucleus | 0.000009 | 0.000013 | N/A | N/A | 0.000016 | 0.000017 | N/A | N/A | 0.000013 | 0.000015 | N/A | N/A |
| Peroxisomes | 0.000093 | 0.000077 | N/A | N/A | 0.000130 | 0.000076 | N/A | N/A | 0.000116 | 0.000077 | N/A | N/A |
| Vacuole | 0.000001 | 0.000002 | N/A | N/A | 0.000004 | 0.000003 | N/A | N/A | 0.000003 | 0.000002 | N/A | N/A |
| Vacuole Periphery | 0.000000 | 0.000000 | N/A | N/A | 0.000001 | 0.000000 | N/A | N/A | 0.000000 | 0.000000 | N/A | N/A |
Localization Changes (T-score)
Change in localization of each mutant relative to its control (e.g. UBP2_WT: ubp2Δ vs wild type). Values highlighted in yellow (increase) or blue (decrease) denote significant changes (marked * in the plot).
| R1 | R2 | R1 & R2 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| UBP2_ |
UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
UBP2_ |
UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
UBP2_ |
UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
|
| Actin | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Bud Neck | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Bud Site | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Cell Periphery | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Cytoplasm | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Cytoplasmic Foci | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Eisosomes | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Endoplasmic Reticulum | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Endosome | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Golgi | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Lipid Particles | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Mitochondria | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Mitotic Spindle | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| None | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Nuclear Periphery | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Nuclear Periphery Foci | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Nucleolus | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Nucleus | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Peroxisomes | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Vacuole | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Vacuole Periphery | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
Endocytosis
| Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
|---|---|---|---|---|
| 37℃ | ||||
| RT |
Cell Cycle Omics
CYCLoPs (Cox5a-GFP)
| Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
|---|
| Gene | Images |
|---|
| Gene | Images |
|---|
Images are not yet available
Images are not yet available
