Standard name | |
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Human Ortholog | |
Description | Cytochrome c heme lyase (holocytochrome c synthase); attaches heme to apo-cytochrome c (Cyc1p or Cyc7p) in mitochondrial intermembrane space; human homolog HCCS implicated in microphthalmia with linear skin defects (MLS), and can complement yeast null mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 10 | 7 | 4 | 4 | 8 | 0 | 0 | – | 1 | 2 | 1 | 19 | 16 | 21 | 19 | 24 | 8 | 5 | 12 | 13 | 10 | 19 | 26 |
Bud | 0 | 3 | 13 | 1 | 11 | 9 | 30 | 19 | – | 21 | 26 | 40 | 1 | 1 | 3 | 0 | 2 | 3 | 3 | 3 | 1 | 4 | 11 | 26 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 3 | 7 | 10 | 8 |
Bud Site | 0 | 0 | 1 | 0 | 1 | 10 | 6 | 7 | – | 7 | 2 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 9 | 2 | 2 | 6 | 1 | 3 | 1 | – | 6 | 6 | 4 | 9 | 5 | 8 | 19 | 16 | 14 | 0 | 1 | 1 | 2 | 2 | 1 |
Cytoplasm | 7 | 61 | 82 | 75 | 65 | 31 | 45 | 39 | – | 37 | 14 | 32 | 75 | 100 | 118 | 73 | 77 | 61 | 9 | 32 | 34 | 26 | 28 | 34 |
Endoplasmic Reticulum | 9 | 14 | 17 | 10 | 7 | 30 | 1 | 4 | – | 8 | 6 | 6 | 45 | 45 | 34 | 5 | 8 | 10 | 8 | 13 | 16 | 19 | 24 | 16 |
Endosome | 1 | 4 | 12 | 4 | 74 | 2 | 29 | 18 | – | 13 | 1 | 6 | 6 | 13 | 13 | 18 | 13 | 9 | 3 | 16 | 20 | 16 | 24 | 51 |
Golgi | 6 | 1 | 0 | 0 | 2 | 1 | 1 | 0 | – | 0 | 2 | 1 | 0 | 8 | 7 | 18 | 14 | 15 | 10 | 3 | 2 | 7 | 13 | 34 |
Mitochondria | 23 | 5 | 77 | 65 | 214 | 331 | 439 | 589 | – | 483 | 378 | 567 | 1 | 8 | 4 | 11 | 7 | 13 | 28 | 49 | 119 | 62 | 61 | 55 |
Nucleus | 0 | 0 | 0 | 0 | 2 | 12 | 9 | 13 | – | 6 | 3 | 5 | 1 | 2 | 3 | 2 | 3 | 1 | 0 | 1 | 0 | 0 | 2 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 2 | 8 | 8 | – | 12 | 9 | 4 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 4 |
Nucleolus | 3 | 0 | 0 | 2 | 5 | 4 | 13 | 21 | – | 12 | 9 | 6 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | – | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 2 | 0 | 6 | 3 | 13 | 14 | – | 18 | 5 | 9 | 2 | 1 | 0 | 2 | 2 | 1 | 1 | 1 | 4 | 1 | 4 | 13 |
Vac/Vac Membrane | 5 | 6 | 15 | 6 | 73 | 49 | 103 | 83 | – | 78 | 37 | 52 | 23 | 45 | 50 | 42 | 37 | 44 | 3 | 18 | 7 | 24 | 42 | 41 |
Unique Cell Count | 43 | 97 | 186 | 140 | 364 | 401 | 537 | 690 | 566 | 408 | 612 | 163 | 215 | 230 | 180 | 175 | 147 | 80 | 166 | 234 | 188 | 253 | 329 | |
Labelled Cell Count | 55 | 113 | 228 | 169 | 472 | 493 | 703 | 817 | 705 | 501 | 740 | 182 | 244 | 262 | 210 | 203 | 180 | 80 | 166 | 234 | 188 | 253 | 329 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.7 | 7.1 | 4.9 | 5.2 | 5.9 | 4.9 | 4.5 | 4.4 | – | 4.5 | 4.3 | 4.5 | 8.3 | 8.1 | 8.0 | 9.6 | 9.8 | 10.4 | 9.0 | 9.6 | 10.5 |
Std Deviation (1e-4) | 2.7 | 0.9 | 0.8 | 1.0 | 1.3 | 0.8 | 1.0 | 0.9 | – | 0.9 | 0.9 | 0.9 | 1.1 | 1.0 | 1.1 | 1.6 | 1.7 | 2.0 | 1.5 | 1.9 | 2.3 |
Intensity Change (Log2) | – | – | – | 0.09 | 0.26 | 0.02 | -0.13 | -0.14 | – | -0.11 | -0.19 | -0.12 | 0.77 | 0.73 | 0.72 | 0.98 | 1.0 | 1.09 | 0.88 | 0.98 | 1.11 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2.8 | 1.6 | 2.2 | 2.5 | 3.4 | 0.7 |
Bud | -2.8 | -2.2 | -2.8 | 0 | 0 | – | 0 | 0 | 0 | -3.0 | -3.5 | -3.0 | -3.6 | -2.8 | -2.1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2.4 | 0 | 0 | 3.9 | 3.5 | 3.6 |
Cytoplasm | 1.7 | -6.6 | -10.4 | -11.0 | -13.5 | – | -12.2 | -12.5 | -13.3 | 0.4 | 0.5 | 1.5 | -0.7 | 0 | -0.5 |
Endoplasmic Reticulum | -0.6 | -3.9 | -0.7 | 0 | 0 | – | 0 | 0 | 0 | 4.5 | 3.3 | 1.7 | -2.6 | -1.7 | -0.8 |
Endosome | -1.5 | 4.2 | 0 | 0 | 0 | – | 0 | 0 | 0 | -1.2 | -0.2 | -0.3 | 1.2 | 0.4 | -0.1 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 4.4 | 3.9 | 4.5 |
Mitochondria | 0.9 | 3.9 | 10.1 | 0 | 0 | – | 0 | 0 | 0 | -9.1 | -9.2 | -10.2 | -7.9 | -8.4 | -6.6 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.4 | 3.6 | 1.5 | 3.5 | 1.5 | – | 2.1 | 0.4 | 0.2 | 1.8 | 3.6 | 3.8 | 4.0 | 3.5 | 5.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.6716 | 4.7763 | 4.1438 | 3.6913 | 3.3166 | 4.3093 | 5.6029 | 6.591 | 6.035 | 5.8547 | 5.1877 | 6.26 | 4.5631 | 5.1598 | 4.9637 | 4.0565 | 4.1315 | 4.7818 |
Actin | 0.0202 | 0.0151 | 0.0287 | 0.0427 | 0.0273 | 0.0183 | 0.0495 | 0.0175 | 0.0374 | 0.0659 | 0.0384 | 0.0313 | 0.0269 | 0.0156 | 0.0332 | 0.0198 | 0.0185 | 0.0194 |
Bud | 0.0026 | 0.0046 | 0.012 | 0.0081 | 0.0087 | 0.0031 | 0.0035 | 0.0033 | 0.0081 | 0.0197 | 0.0096 | 0.0033 | 0.0036 | 0.0062 | 0.0085 | 0.0197 | 0.0098 | 0.0052 |
Bud Neck | 0.0042 | 0.0021 | 0.0032 | 0.0032 | 0.0091 | 0.0099 | 0.0027 | 0.0028 | 0.007 | 0.003 | 0.0026 | 0.0158 | 0.0045 | 0.0044 | 0.0039 | 0.0037 | 0.0058 | 0.0138 |
Bud Periphery | 0.003 | 0.0029 | 0.0163 | 0.0184 | 0.0134 | 0.0035 | 0.0042 | 0.0023 | 0.0114 | 0.0489 | 0.0093 | 0.0045 | 0.0048 | 0.005 | 0.0087 | 0.0274 | 0.0131 | 0.0081 |
Bud Site | 0.0142 | 0.0526 | 0.0258 | 0.0041 | 0.0068 | 0.0039 | 0.0217 | 0.0388 | 0.0335 | 0.0079 | 0.0071 | 0.003 | 0.0201 | 0.0499 | 0.0262 | 0.0136 | 0.0064 | 0.0033 |
Cell Periphery | 0.0021 | 0.0019 | 0.0056 | 0.0092 | 0.0014 | 0.0009 | 0.0041 | 0.0033 | 0.0071 | 0.0031 | 0.0013 | 0.0023 | 0.0041 | 0.0029 | 0.0048 | 0.0018 | 0.0029 | 0.0015 |
Cytoplasm | 0.4055 | 0.5184 | 0.4807 | 0.5762 | 0.4202 | 0.4676 | 0.3132 | 0.5629 | 0.4477 | 0.4751 | 0.3769 | 0.4032 | 0.278 | 0.5326 | 0.421 | 0.4486 | 0.4474 | 0.3916 |
Cytoplasmic Foci | 0.1234 | 0.1131 | 0.1057 | 0.0632 | 0.1561 | 0.1212 | 0.1018 | 0.0964 | 0.0972 | 0.0678 | 0.1103 | 0.1094 | 0.1162 | 0.0806 | 0.1092 | 0.1009 | 0.1154 | 0.1112 |
Eisosomes | 0.0005 | 0.0002 | 0.0007 | 0.0004 | 0.0003 | 0.0001 | 0.0009 | 0.0002 | 0.001 | 0.0005 | 0.0003 | 0.0003 | 0.0005 | 0.0002 | 0.0005 | 0.0002 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.0289 | 0.0299 | 0.0276 | 0.0178 | 0.0054 | 0.0342 | 0.0803 | 0.0293 | 0.0367 | 0.0217 | 0.032 | 0.0303 | 0.0817 | 0.04 | 0.037 | 0.0297 | 0.02 | 0.0386 |
Endosome | 0.2015 | 0.1592 | 0.133 | 0.1144 | 0.1472 | 0.1845 | 0.1671 | 0.1074 | 0.1073 | 0.0822 | 0.1437 | 0.1488 | 0.2479 | 0.1377 | 0.1485 | 0.1179 | 0.1167 | 0.1974 |
Golgi | 0.0594 | 0.0566 | 0.0583 | 0.0436 | 0.0772 | 0.0629 | 0.0915 | 0.0695 | 0.0921 | 0.0647 | 0.1206 | 0.0873 | 0.0802 | 0.0589 | 0.0889 | 0.063 | 0.0939 | 0.0929 |
Lipid Particles | 0.0092 | 0.0013 | 0.0038 | 0.0017 | 0.003 | 0.0037 | 0.0181 | 0.0013 | 0.0028 | 0.002 | 0.0096 | 0.0052 | 0.0117 | 0.0044 | 0.0063 | 0.0083 | 0.0049 | 0.004 |
Mitochondria | 0.0444 | 0.0108 | 0.0535 | 0.0679 | 0.0585 | 0.0485 | 0.0674 | 0.0427 | 0.0851 | 0.1201 | 0.1005 | 0.1155 | 0.052 | 0.0369 | 0.0756 | 0.0908 | 0.0949 | 0.0823 |
None | 0.0321 | 0.0127 | 0.0211 | 0.0078 | 0.0328 | 0.0104 | 0.0298 | 0.0073 | 0.0029 | 0.0037 | 0.0019 | 0.0165 | 0.0102 | 0.003 | 0.0034 | 0.0042 | 0.0031 | 0.0045 |
Nuclear Periphery | 0.0051 | 0.0029 | 0.0025 | 0.0026 | 0.0011 | 0.003 | 0.0081 | 0.0016 | 0.0016 | 0.0012 | 0.0018 | 0.0011 | 0.0121 | 0.0025 | 0.0022 | 0.0023 | 0.0013 | 0.0036 |
Nucleolus | 0.0005 | 0.0002 | 0.0004 | 0.0006 | 0.0025 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0008 | 0.0002 | 0.0003 | 0.0013 | 0.0002 | 0.0002 |
Nucleus | 0.0029 | 0.0024 | 0.0023 | 0.0035 | 0.0038 | 0.0024 | 0.003 | 0.0017 | 0.0014 | 0.0012 | 0.001 | 0.0013 | 0.0053 | 0.0024 | 0.0022 | 0.0028 | 0.0018 | 0.0028 |
Peroxisomes | 0.0157 | 0.0035 | 0.0079 | 0.0042 | 0.0114 | 0.0085 | 0.015 | 0.0039 | 0.0084 | 0.0034 | 0.0222 | 0.011 | 0.0083 | 0.0029 | 0.0075 | 0.0255 | 0.031 | 0.0061 |
Punctate Nuclear | 0.0042 | 0.0007 | 0.0018 | 0.0006 | 0.0054 | 0.0015 | 0.0041 | 0.0006 | 0.0006 | 0.0004 | 0.0005 | 0.0006 | 0.0042 | 0.0007 | 0.001 | 0.0036 | 0.0014 | 0.0017 |
Vacuole | 0.0167 | 0.007 | 0.0071 | 0.0066 | 0.0066 | 0.0083 | 0.0106 | 0.0053 | 0.0084 | 0.0057 | 0.0061 | 0.0065 | 0.0219 | 0.0108 | 0.0082 | 0.0109 | 0.008 | 0.008 |
Vacuole Periphery | 0.0039 | 0.0019 | 0.0022 | 0.0033 | 0.0017 | 0.0033 | 0.0032 | 0.0016 | 0.002 | 0.0018 | 0.0041 | 0.0027 | 0.0051 | 0.0021 | 0.003 | 0.0037 | 0.0033 | 0.0035 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.3467 | 34.097 | 31.6268 | 24.0485 | 15.0126 | 32.6754 | 21.2393 | 30.3322 | 43.5955 | 21.3647 |
Translational Efficiency | 2.2908 | 1.3117 | 1.5109 | 2.3992 | 2.0289 | 1.3433 | 2.4583 | 2.309 | 1.5641 | 1.7537 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1865 | 1845 | 1239 | 1512 | 2478 | 1266 | 1447 | 1158 | 4343 | 3111 | 2686 | 2670 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 797.74 | 900.73 | 995.98 | 1128.87 | 820.24 | 841.29 | 1124.83 | 1126.87 | 810.58 | 876.54 | 1065.39 | 1128.00 |
Standard Deviation | 104.55 | 126.77 | 132.75 | 170.82 | 107.56 | 128.76 | 142.39 | 209.30 | 106.86 | 130.88 | 152.24 | 188.48 |
Intensity Change Log 2 | — | 0.175176 | 0.320198 | 0.500889 | — | 0.036557 | 0.455589 | 0.458203 | — | 0.106567 | 0.390422 | 0.479407 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000942 | 0.004681 | 0.006428 | 0.021188 | 0.001063 | 0.003991 | 0.012753 | 0.026584 | 0.001011 | 0.004401 | 0.009835 | 0.023528 |
Bud Neck | 0.025500 | 0.024360 | 0.018337 | 0.008961 | 0.029270 | 0.027620 | 0.024071 | 0.008257 | 0.027651 | 0.025687 | 0.021426 | 0.008655 |
Bud Site | 0.045576 | 0.103082* | 0.082633 | 0.162172* | 0.045945 | 0.087234 | 0.197798* | 0.217330* | 0.045787 | 0.096633 | 0.144674* | 0.186095* |
Cell Periphery | 0.000337 | 0.000536 | 0.000282 | 0.000311 | 0.000210 | 0.000754 | 0.000321 | 0.000539 | 0.000265 | 0.000625 | 0.000303 | 0.000410 |
Cytoplasm | 0.078640 | 0.103832* | 0.286530* | 0.118894* | 0.151528* | 0.096374 | 0.209811* | 0.132106* | 0.120228* | 0.100797* | 0.245200* | 0.124624* |
Cytoplasmic Foci | 0.230703* | 0.268771* | 0.205990* | 0.152872 | 0.324954* | 0.247193* | 0.122580 | 0.171469 | 0.284480* | 0.259990* | 0.161055 | 0.160938 |
Eisosomes | 0.000279 | 0.000302 | 0.000099 | 0.000083 | 0.000237 | 0.000449 | 0.000099 | 0.000090 | 0.000255 | 0.000362 | 0.000099 | 0.000086 |
Endoplasmic Reticulum | 0.009085 | 0.005515 | 0.014477 | 0.002956 | 0.009818 | 0.005344 | 0.001957 | 0.003294 | 0.009503 | 0.005445 | 0.007733 | 0.003103 |
Endosome | 0.112483 | 0.106002 | 0.057680 | 0.053071 | 0.092668 | 0.125235 | 0.060825 | 0.064045 | 0.101177 | 0.113829 | 0.059374 | 0.057831 |
Golgi | 0.098282 | 0.121939* | 0.019604 | 0.063975 | 0.068615 | 0.127103* | 0.049486 | 0.099205 | 0.081355 | 0.124041* | 0.035702 | 0.079255 |
Lipid Particles | 0.015716 | 0.006787 | 0.009906 | 0.004684 | 0.016010 | 0.011893 | 0.002020 | 0.004839 | 0.015884 | 0.008865 | 0.005658 | 0.004751 |
Mitochondria | 0.260164* | 0.131049* | 0.007838 | 0.023560 | 0.110334* | 0.147612* | 0.057882 | 0.023937 | 0.174675* | 0.137789* | 0.034797 | 0.023724 |
Mitotic Spindle | 0.003437 | 0.011592 | 0.030841 | 0.273777* | 0.004899 | 0.008381 | 0.054151 | 0.151131* | 0.004271 | 0.010285 | 0.043399 | 0.220584* |
None | 0.000753 | 0.000384 | 0.000952 | 0.000188 | 0.000717 | 0.000426 | 0.000195 | 0.000272 | 0.000733 | 0.000401 | 0.000544 | 0.000225 |
Nuclear Periphery | 0.000331 | 0.000146 | 0.001776 | 0.000322 | 0.000310 | 0.000175 | 0.000595 | 0.000123 | 0.000319 | 0.000158 | 0.001139 | 0.000236 |
Nuclear Periphery Foci | 0.000484 | 0.000693 | 0.006322 | 0.002666 | 0.000525 | 0.000489 | 0.000716 | 0.001641 | 0.000507 | 0.000610 | 0.003302 | 0.002221 |
Nucleolus | 0.000549 | 0.000872 | 0.001887 | 0.000214 | 0.000707 | 0.000716 | 0.000666 | 0.000165 | 0.000639 | 0.000808 | 0.001229 | 0.000193 |
Nucleus | 0.005309 | 0.003873 | 0.043200 | 0.001711 | 0.012443 | 0.003129 | 0.004348 | 0.001363 | 0.009379 | 0.003570 | 0.022270 | 0.001560 |
Peroxisomes | 0.012842 | 0.024330 | 0.015203 | 0.024572 | 0.012410 | 0.022887 | 0.019240 | 0.023171 | 0.012596 | 0.023743 | 0.017378 | 0.023964 |
Vacuole | 0.065103 | 0.059195 | 0.180798 | 0.072109 | 0.094732 | 0.056686 | 0.156704 | 0.056205 | 0.082009 | 0.058174 | 0.167818 | 0.065211 |
Vacuole Periphery | 0.033486 | 0.022056 | 0.009219 | 0.011715 | 0.022606 | 0.026311 | 0.023782 | 0.014233 | 0.027278 | 0.023788 | 0.017064 | 0.012807 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -7.51 | -7.41 | -10.00 | -7.80 | -6.65 | -4.61 | -7.73 | -10.32 | -8.84 | -4.83 | -8.59 | -10.00 | -14.34 | -11.74 | -7.49 |
Bud Neck | 0.57 | 3.47 | 9.31 | 9.83 | 6.11 | 0.70 | 2.60 | 14.05 | 9.44 | 10.49 | 1.31 | 4.34 | 16.47 | 13.68 | 11.79 |
Bud Site | -13.52 | -7.77 | -19.70 | -8.76 | -12.08 | -9.55 | -23.98* | -23.84 | -16.14 | -2.58 | -16.78 | -23.57 | -30.62 | -17.22 | -7.63 |
Cell Periphery | -2.87 | 2.12 | 1.18 | 3.37 | -0.40 | -7.12 | -2.67 | -4.13 | 2.14 | -2.55 | -7.07 | -1.47 | -3.44 | 3.72 | -2.33 |
Cytoplasm | -5.22 | -24.89 | -8.06 | -3.43 | 17.64 | 9.61 | -8.55 | 1.87 | -5.75 | 8.77 | 5.00 | -22.80 | -2.36 | -6.36 | 18.74 |
Cytoplasmic Foci | -6.18 | 4.71 | 13.65 | 19.36 | 7.30 | 11.75 | 37.00 | 24.43 | 11.05 | -7.80 | 5.30 | 28.70 | 28.69 | 21.91 | -0.19 |
Eisosomes | -0.76 | 9.46 | 10.66 | 9.42 | 3.26 | -3.73 | 13.69 | 15.99 | 6.38 | 1.04 | -3.77 | 15.73 | 17.78 | 10.33 | 2.96 |
Endoplasmic Reticulum | 2.85 | -3.51 | 5.61 | 2.96 | 9.24 | 3.13 | 7.61 | 5.29 | 1.69 | -1.46 | 4.31 | 1.90 | 7.83 | 3.27 | 6.77 |
Endosome | 1.50 | 14.76 | 16.69 | 15.11 | 1.60 | -7.20 | 10.05 | 8.83 | 13.80 | -0.59 | -4.10 | 17.37 | 18.32 | 20.28 | 1.04 |
Golgi | -4.70 | 22.61 | 9.21 | 13.31 | -11.83 | -11.70 | 6.38 | -4.91 | 5.28 | -8.82 | -12.39 | 19.46 | 2.55 | 13.00 | -12.87 |
Lipid Particles | 6.99 | 4.19 | 8.63 | 2.33 | 6.01 | 2.56 | 14.93 | 10.16 | 4.67 | -4.95 | 7.35 | 12.75 | 13.47 | 5.09 | 1.41 |
Mitochondria | 16.70 | 40.08* | 36.28* | 21.81 | -6.52 | -5.32 | 11.74 | 21.19 | 19.18 | 8.30 | 7.31 | 35.79 | 39.89* | 29.03 | 4.76 |
Mitotic Spindle | -6.51 | -10.55 | -29.82* | -28.71* | -25.70* | -2.32 | -11.91 | -18.72 | -18.15 | -10.74 | -6.06 | -15.32 | -34.56 | -33.40* | -25.90 |
None | 1.32 | -0.56 | 2.03 | 3.88 | 5.00 | 1.96 | 4.56 | 3.52 | 1.56 | -1.18 | 2.31 | 1.33 | 3.70 | 3.39 | 4.19 |
Nuclear Periphery | 3.35 | -4.39 | -0.11 | -2.54 | 4.31 | 2.68 | -1.34 | 4.97 | 0.94 | 1.89 | 4.64 | -3.94 | 1.32 | -2.01 | 4.19 |
Nuclear Periphery Foci | -1.55 | -9.77 | -3.59 | -3.09 | 4.19 | 0.25 | -1.97 | -4.77 | -4.89 | -3.19 | -1.30 | -9.74 | -4.90 | -4.42 | 2.22 |
Nucleolus | -1.21 | -4.02 | 1.77 | 2.18 | 4.90 | -0.08 | 0.00 | 6.36 | 2.75 | 1.68 | -0.99 | -2.71 | 4.96 | 3.22 | 4.47 |
Nucleus | 1.69 | -15.91 | 3.63 | 2.68 | 17.40 | 12.16 | 8.60 | 15.45 | 3.62 | 5.94 | 10.25 | -11.09 | 13.68 | 4.18 | 17.29 |
Peroxisomes | -7.73 | -1.53 | -6.31 | 0.31 | -4.87 | -7.58 | -4.30 | -6.66 | 0.15 | -1.94 | -10.92 | -4.40 | -9.15 | 0.36 | -4.58 |
Vacuole | 1.49 | -19.12 | -3.90 | -5.04 | 14.77 | 9.37 | -11.15 | 7.74 | -1.02 | 15.84 | 8.66 | -20.70 | 2.53 | -4.64 | 20.90 |
Vacuole Periphery | 6.78 | 16.49 | 13.32 | 7.67 | -1.73 | -2.26 | -0.01 | 5.84 | 6.81 | 4.62 | 3.10 | 9.50 | 13.66 | 10.17 | 3.51 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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