Standard name
Human Ortholog
Description tRNA:pseudouridine synthase; introduces pseudouridines at position 38 or 39 in tRNA; also responsible for pseudouracil modification of some mRNAs; important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0 0.06 0 0 0 0 0 0 0 0.06 0.06 0.12 0.07 0.05 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0 0.06 0.13 0.11 0.31 0.43 0.38 0.47 0.55 0.64 0.59 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.92 0.94 0.92 0.9 0.88 0.82 0.76 0.78 0.75 0.74 0.73 0.71 0.91 0.9 0.83 0.87 0.81 0.86 0.83 0.92 0.74 0.87 0.73 0.62
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0.05 0 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0.06 0.12 0.1 0.09 0 0.21 0.08 0.18 0.31
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 1 0 0 0
Bud 0 0 0 0 1 1 3 3 2 2 1 5 0 0 0 0 0 0 3 2 1 0 1 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 1 1 7 9 4 5 5 12 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 7 3 1 14 4 13 11 6 2 0 5 12 20 43 14 12 10 1 0 0 1 5 3
Endoplasmic Reticulum 0 0 1 0 0 0 1 2 0 0 0 0 0 1 2 3 9 1 0 0 0 0 0 0
Endosome 1 0 1 1 0 0 0 0 0 0 0 0 0 3 3 0 1 0 3 0 2 0 1 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 0 2 0 0 1
Mitochondria 22 1 12 17 25 76 188 142 148 212 116 177 1 0 2 3 6 0 5 0 0 0 3 2
Nucleus 194 141 190 122 197 202 333 295 239 283 133 214 188 295 308 176 183 168 176 140 154 106 174 184
Nuclear Periphery 2 0 2 2 0 3 6 6 5 4 3 5 2 1 1 0 2 0 0 0 0 0 0 1
Nucleolus 0 1 2 4 2 9 19 13 16 15 7 26 1 0 3 0 5 0 0 0 0 0 3 3
Peroxisomes 0 0 0 0 0 1 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 1 1 0 0 0 1 0 2 2 0 1 0 0 0 0 0 1 1
Vac/Vac Membrane 7 3 8 3 9 4 12 18 9 15 8 13 6 10 11 13 28 19 19 6 44 9 41 90
Unique Cell Count 211 150 206 135 224 246 439 376 318 383 182 300 207 328 369 202 225 195 214 153 209 123 239 296
Labelled Cell Count 229 153 219 150 249 302 583 500 429 538 273 458 210 334 378 209 248 199 214 153 209 123 239 296


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 8.3 7.3 6.4 6.2 5.2 4.9 4.7 4.5 4.2 4.0 4.0 8.7 8.5 8.0 10.0 9.6 9.3 8.4 8.8 9.5
Std Deviation (1e-4) 1.2 1.4 2.1 1.2 1.6 1.2 1.5 1.1 1.2 0.7 0.9 0.9 1.8 1.6 2.2 2.1 2.1 2.1 1.4 1.4 1.8
Intensity Change (Log2) – – – -0.18 -0.22 -0.47 -0.56 -0.62 -0.7 -0.8 -0.87 -0.86 0.26 0.22 0.14 0.46 0.41 0.36 0.22 0.28 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.5 0 1.1 0 0 0 0 0 2.4 2.6 4.3 2.8 2.2 2.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.2 2.0 6.7 9.5 0 0 0 0 0 -3.1 0 -3.9 -2.3 -1.6 -3.4
Nucleus -0.6 -1.5 -3.2 -5.0 -4.3 -5.0 -5.3 -5.0 -5.8 -0.5 -0.9 -3.0 -1.7 -3.3 -2.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1.8 2.2 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0.1 -1.5 -0.8 0.5 -0.7 0 0.3 0.2 -0.6 -0.5 -0.6 1.2 3.2 2.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.9806 6.1437 5.9239 5.6566 5.0927 5.5962 4.1951 5.1635 4.4986 4.711 4.2901 4.5121 3.6101 3.5438 3.2257 3.3005 2.997 3.0541
Actin 0.0055 0 0 0 0 0 0.0093 0.0002 0.0009 0.0036 0.0108 0.0015 0.0177 0 0.0028 0.0014 0.0009 0.0012
Bud 0 0 0 0 0 0 0.001 0 0.0002 0.0002 0.0004 0.0007 0.0007 0 0 0.0001 0.0006 0.0001
Bud Neck 0.0003 0.0001 0.0002 0 0.0002 0.0005 0.0065 0 0.0003 0.0002 0.0002 0.0021 0.0045 0 0 0.0001 0.0004 0.0009
Bud Periphery 0.0001 0 0 0 0 0 0.0016 0.0001 0.0007 0.0002 0.0005 0.0013 0.0017 0 0 0.0002 0.0013 0.0004
Bud Site 0.0001 0.0001 0 0 0 0 0.0052 0 0.0004 0.0005 0.0011 0.001 0.0013 0 0.0001 0.0003 0.0005 0.0001
Cell Periphery 0.0001 0 0 0 0 0 0.0005 0 0.0001 0 0.0001 0.0002 0.0007 0 0 0 0.0001 0.0001
Cytoplasm 0.0004 0.0004 0.0002 0.0007 0.0001 0 0.0131 0.0001 0.0032 0.0022 0.001 0.0007 0.0007 0.0001 0 0.0003 0.0007 0.0001
Cytoplasmic Foci 0.0003 0 0 0 0 0 0.0173 0 0.0027 0.0333 0.0255 0.0042 0.0073 0 0.0003 0.0004 0.0093 0.0004
Eisosomes 0 0 0 0 0 0 0.0001 0 0 0 0.0001 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0001 0 0 0 0 0 0.0034 0 0.0009 0.0005 0.0006 0.0006 0.0007 0 0 0.0014 0.0011 0.0003
Endosome 0.0006 0 0 0 0 0 0.0185 0.0003 0.0063 0.0327 0.0351 0.0089 0.0044 0 0.0001 0.0114 0.0269 0.0021
Golgi 0.0009 0 0 0 0 0 0.004 0.0001 0.001 0.0233 0.0374 0.0047 0.0026 0 0.0003 0.0015 0.0045 0.001
Lipid Particles 0.0013 0 0 0 0 0 0.0122 0.0001 0.0012 0.0085 0.018 0.0109 0.0112 0 0.0002 0.0021 0.0123 0.0011
Mitochondria 0.0006 0.0003 0.0001 0.0001 0.0002 0.0001 0.0099 0.0079 0.0096 0.0054 0.0132 0.0137 0.0101 0.0003 0.0037 0.0025 0.0451 0.0069
None 0.0002 0 0 0.0005 0 0 0.0031 0.0001 0.0002 0.0002 0.0004 0.0002 0.0005 0 0 0.0005 0.0002 0.0001
Nuclear Periphery 0.0025 0.0035 0.001 0.0022 0.0022 0.0003 0.0463 0.0032 0.0096 0.0019 0.0065 0.0088 0.0016 0.0006 0.0012 0.0161 0.0048 0.0034
Nucleolus 0.0054 0.0029 0.0031 0.0029 0.0177 0.0113 0.011 0.0026 0.0037 0.0018 0.0084 0.0129 0.013 0.0066 0.0025 0.0017 0.0102 0.0119
Nucleus 0.9784 0.9915 0.9947 0.9926 0.978 0.9873 0.7838 0.9846 0.9509 0.8796 0.8272 0.9187 0.9046 0.992 0.9862 0.9558 0.8674 0.967
Peroxisomes 0.0006 0 0 0 0 0 0.0087 0.0001 0.0004 0.0025 0.009 0.0017 0.0013 0 0.0024 0.0002 0.0007 0.0007
Punctate Nuclear 0.0018 0.0007 0.0001 0.0008 0.0014 0.0002 0.0265 0.0001 0.005 0.0022 0.0028 0.0023 0.0105 0 0 0.0008 0.002 0.0003
Vacuole 0.0006 0.0002 0.0004 0 0.0001 0.0001 0.0131 0.0001 0.0018 0.0005 0.0008 0.0027 0.003 0.0001 0 0.0013 0.0066 0.0009
Vacuole Periphery 0.0002 0.0001 0.0001 0 0 0 0.0048 0.0003 0.0012 0.0007 0.0011 0.0022 0.0019 0.0001 0 0.0022 0.0043 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.6235 16.3283 15.9715 21.1838 26.9114 7.4681 18.7608 16.9674 16.7884 18.7596
Translational Efficiency 1.3021 0.8366 0.7694 0.5992 0.4922 1.4349 0.5858 0.4885 0.7044 0.5996

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
87 301 288 546 1758 2072 519 2381 1845 2373 807 2927

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 934.21 1021.96 1188.35 1024.30 851.38 928.39 1048.25 991.76 855.29 940.26 1098.25 997.83
Standard Deviation 635.11 157.96 134.80 143.92 129.79 131.62 141.23 147.06 188.10 138.78 154.33 147.02
Intensity Change Log 2 — 0.129520 0.347141 0.132820 — 0.124928 0.300108 0.220188 — 0.127332 0.324906 0.175138

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003252 0.001765 0.001471 0.002371 0.000701 0.000797 0.000992 0.001175 0.000822 0.000920 0.001163 0.001398
Bud Neck 0.030293 0.024983 0.017022 0.036197 0.010157 0.015562 0.006641 0.044005 0.011107 0.016757 0.010346 0.042548
Bud Site 0.027740 0.004648 0.001717 0.012590 0.002092 0.003219 0.001588 0.011869 0.003302 0.003401 0.001634 0.012003
Cell Periphery 0.000431 0.000216 0.000079 0.000287 0.000677 0.000476 0.000156 0.000257 0.000666 0.000443 0.000129 0.000263
Cytoplasm 0.005562 0.001102 0.001521 0.023821 0.002672 0.002232 0.005539 0.059031 0.002808 0.002089 0.004105 0.052463
Cytoplasmic Foci 0.014833 0.000095 0.000123 0.001648 0.000747 0.001154 0.000389 0.001597 0.001411 0.001020 0.000294 0.001607
Eisosomes 0.000145 0.000056 0.000061 0.000049 0.000044 0.000046 0.000043 0.000030 0.000049 0.000047 0.000050 0.000033
Endoplasmic Reticulum 0.050635 0.020425 0.011735 0.025291 0.021772 0.021322 0.010374 0.014219 0.023133 0.021208 0.010860 0.016284
Endosome 0.007553 0.000465 0.000331 0.002014 0.000853 0.001563 0.000726 0.002032 0.001169 0.001424 0.000585 0.002029
Golgi 0.005318 0.000062 0.000066 0.000530 0.000273 0.000623 0.000063 0.000859 0.000511 0.000552 0.000064 0.000798
Lipid Particles 0.007607 0.000240 0.000220 0.000770 0.000268 0.001055 0.000430 0.000550 0.000614 0.000951 0.000355 0.000591
Mitochondria 0.045283 0.002805 0.002086 0.004501 0.002109 0.005528 0.001347 0.006154 0.004145 0.005183 0.001611 0.005846
Mitotic Spindle 0.024752 0.001085 0.000085 0.018074 0.000819 0.004475 0.002195 0.020805 0.001948 0.004045 0.001442 0.020295
None 0.001997 0.001653 0.000489 0.003748 0.002890 0.002402 0.003825 0.003823 0.002848 0.002307 0.002634 0.003809
Nuclear Periphery 0.001187 0.000672 0.000463 0.003391 0.001355 0.002432 0.000701 0.005013 0.001347 0.002209 0.000616 0.004711
Nuclear Periphery Foci 0.003023 0.000433 0.000098 0.001181 0.000395 0.001488 0.000330 0.000755 0.000519 0.001354 0.000247 0.000834
Nucleolus 0.024363 0.005580 0.003099 0.002703 0.006925 0.007118 0.003865 0.002208 0.007748 0.006923 0.003591 0.002301
Nucleus 0.715638* 0.930538* 0.952869* 0.841853* 0.941840* 0.921605* 0.955520* 0.806045* 0.931173* 0.922738* 0.954574* 0.812725*
Peroxisomes 0.007953 0.000628 0.000551 0.000739 0.000526 0.001221 0.000459 0.000539 0.000876 0.001146 0.000492 0.000576
Vacuole 0.018603 0.002290 0.005747 0.017840 0.002364 0.004203 0.004701 0.018230 0.003130 0.003960 0.005074 0.018157
Vacuole Periphery 0.003831 0.000260 0.000166 0.000402 0.000519 0.001477 0.000117 0.000805 0.000675 0.001323 0.000134 0.000730

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.39 2.93 0.61 -3.24 -5.31 -0.93 -4.02 -3.81 -3.09 -0.63 -1.00 -4.84 -5.47 -4.64 -1.75
Bud Neck 0.50 1.57 -1.99 -3.95 -7.05 -4.03 1.44 -19.17 -16.12 -20.15 -4.27 -0.70 -20.34 -16.78 -19.50
Bud Site 2.24 2.53 1.31 -4.59 -7.40 -2.27 -0.13 -10.24 -8.44 -9.45 -0.30 1.83 -9.34 -9.70 -11.74
Cell Periphery 2.29 4.01 1.69 -1.44 -7.95 1.65 4.87 4.48 3.67 -1.64 1.96 5.37 4.46 3.45 -3.70
Cytoplasm 2.18 1.83 -5.14 -8.21 -7.71 0.64 -1.68 -22.00 -22.36 -16.77 1.19 -1.16 -22.83 -23.50 -19.45
Cytoplasmic Foci 2.40 2.40 2.17 -3.23 -3.16 -0.91 0.99 -2.49 -1.62 -4.81 0.76 2.55 -0.69 -2.08 -6.01
Eisosomes 5.01 4.68 4.52 -1.62 -0.53 -0.47 -1.16 2.06 3.86 3.36 0.37 -1.47 1.78 2.14 4.21
Endoplasmic Reticulum 4.11 5.37 3.07 -3.10 -8.46 0.31 8.60 5.68 5.84 -3.98 1.45 9.81 4.66 3.63 -7.28
Endosome 2.73 2.77 2.01 -6.43 -7.07 -2.62 -0.40 -3.10 -0.66 -1.20 -1.04 0.77 -2.53 -1.54 -2.54
Golgi 2.16 2.15 2.02 -2.27 -2.20 -1.62 1.24 -1.15 0.28 -2.23 -0.28 2.33 -0.12 0.16 -2.55
Lipid Particles 2.10 2.10 1.77 -6.96 -7.62 -2.88 -2.67 -7.53 0.92 -3.09 -1.32 0.93 -1.91 0.24 -6.63
Mitochondria 2.86 2.90 2.70 -4.34 -6.90 -4.10 1.63 -8.59 -0.66 -8.78 -1.09 3.03 -2.29 -0.94 -10.05
Mitotic Spindle 1.80 1.90 -0.35 -5.17 -5.52 -3.63 -1.24 -10.02 -7.75 -7.05 -2.15 -0.17 -10.42 -9.05 -9.42
None 0.52 3.83 -2.36 -2.46 -6.88 0.89 -0.80 -2.14 -3.84 -0.39 1.06 0.57 -2.09 -4.22 -2.46
Nuclear Periphery 1.42 1.92 -8.09 -12.07 -13.43 -3.15 2.44 -15.73 -8.98 -17.13 -2.85 3.88 -17.39 -10.34 -20.47
Nuclear Periphery Foci 2.27 2.74 0.70 -3.98 -6.97 -2.49 -1.42 -5.20 0.28 -0.84 -2.18 -0.60 -5.66 -0.40 -2.82
Nucleolus 2.83 3.22 3.16 2.42 -0.63 -0.33 3.12 3.76 4.82 0.19 0.95 4.92 4.93 5.37 -0.24
Nucleus -6.01 -6.67 -2.01 11.18 14.18 4.82 -0.11 27.63 21.97 21.61 2.17 -2.69 26.45 24.40 26.99
Peroxisomes 2.92 2.95 2.89 -0.38 -0.83 -2.45 -0.05 -0.90 1.67 -0.68 -1.05 1.77 0.85 1.65 -0.84
Vacuole 3.86 2.65 -3.57 -9.53 -7.62 -3.48 -3.22 -16.01 -14.38 -6.62 -1.69 -3.31 -17.89 -16.97 -9.84
Vacuole Periphery 3.81 3.91 3.66 -2.09 -4.90 -2.74 4.07 -0.66 2.44 -4.44 -2.09 5.17 0.73 2.44 -4.82
Standard name
Human Ortholog
Description tRNA:pseudouridine synthase; introduces pseudouridines at position 38 or 39 in tRNA; also responsible for pseudouracil modification of some mRNAs; important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability
Localization
Cell Percentages nucleus (91%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Deg1

Deg1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Deg1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available