Standard name | |
---|---|
Human Ortholog | |
Description | Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p); human homolog CNDP2 can complement yeast dug1 mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 3 | 1 | 0 | 2 | 15 | 16 | 20 | 30 | 29 | 32 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 |
Bud | 1 | 0 | 3 | 1 | 1 | 1 | 1 | 3 | 4 | 16 | 7 | 10 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 13 | 0 | 5 | 9 | 8 | 5 | 16 | 18 | 9 | 12 | 10 | 9 | 8 | 18 | 15 | 19 | 10 | 12 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 238 | 35 | 223 | 220 | 301 | 326 | 425 | 354 | 211 | 241 | 141 | 156 | 221 | 475 | 537 | 183 | 222 | 146 | 238 | 60 | 210 | 151 | 367 | 460 |
Endoplasmic Reticulum | 3 | 0 | 2 | 2 | 2 | 11 | 59 | 57 | 40 | 52 | 35 | 31 | 2 | 1 | 4 | 7 | 7 | 5 | 1 | 0 | 7 | 1 | 3 | 14 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 1 |
Golgi | 1 | 0 | 1 | 0 | 0 | 3 | 3 | 20 | 18 | 14 | 35 | 24 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 3 | 1 | 1 | 1 |
Mitochondria | 2 | 1 | 1 | 4 | 0 | 3 | 1 | 6 | 31 | 45 | 39 | 51 | 0 | 2 | 0 | 4 | 0 | 4 | 0 | 0 | 3 | 0 | 0 | 1 |
Nucleus | 2 | 0 | 1 | 2 | 0 | 2 | 1 | 0 | 0 | 3 | 3 | 2 | 0 | 2 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 3 | 2 |
Nucleolus | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 4 | 1 | 9 | 9 | 7 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 0 | 1 | 0 | 3 | 11 | 31 | 8 | 32 | 26 | 40 | 0 | 0 | 2 | 4 | 3 | 4 | 2 | 2 | 3 | 2 | 3 | 6 |
Unique Cell Count | 246 | 36 | 230 | 226 | 304 | 343 | 509 | 475 | 303 | 400 | 282 | 321 | 225 | 482 | 548 | 195 | 227 | 155 | 249 | 66 | 240 | 164 | 390 | 500 |
Labelled Cell Count | 261 | 38 | 240 | 240 | 312 | 356 | 533 | 509 | 342 | 456 | 334 | 363 | 233 | 501 | 562 | 220 | 245 | 172 | 249 | 66 | 240 | 164 | 390 | 500 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 18.0 | 17.4 | 18.0 | 19.3 | 26.8 | 26.9 | 28.3 | 26.8 | 25.6 | 21.6 | 23.3 | 22.8 | 23.0 | 22.1 | 22.1 | 24.8 | 31.5 | 34.0 | 19.7 | 19.1 | 20.3 |
Std Deviation (1e-4) | 3.5 | 3.2 | 3.3 | 3.8 | 5.7 | 6.0 | 6.6 | 7.0 | 7.5 | 6.5 | 7.4 | 6.9 | 4.5 | 4.7 | 4.3 | 8.6 | 10.5 | 12.6 | 3.5 | 3.4 | 3.9 |
Intensity Change (Log2) | – | – | – | 0.1 | 0.58 | 0.58 | 0.65 | 0.57 | 0.51 | 0.27 | 0.37 | 0.34 | 0.36 | 0.3 | 0.29 | 0.46 | 0.81 | 0.92 | 0.13 | 0.09 | 0.17 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 1.3 | 1.6 | 3.0 | 3.4 | 4.2 | 4.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1.9 | 0 | 1.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.1 | 0.3 | 0 | 0.7 | 1.1 | 0.6 | 0.6 | 0.9 | 0.5 | 0.9 | 1.1 | 0.5 | 3.4 | 1.3 | 2.6 |
Cytoplasm | 0.2 | 1.7 | -1.1 | -5.2 | -7.2 | -8.0 | -10.1 | -11.7 | -12.1 | 0.9 | 1.4 | 0.9 | -1.5 | 0.6 | -1.3 |
Endoplasmic Reticulum | 0 | 0 | 1.8 | 4.9 | 5.0 | 5.2 | 5.2 | 5.0 | 4.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 2.8 | 3.4 | 2.4 | 5.3 | 3.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 4.7 | 5.0 | 5.6 | 6.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 4.0 | 0 | 4.4 | 4.7 | 5.6 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 12.7261 | 15.4194 | 14.8732 | 15.3353 | 14.2264 | 14.4342 | 12.4564 | 16.3832 | 12.8628 | 11.2614 | 10.3281 | 13.4449 | 13.4572 | 16.3437 | 15.0317 | 14.8338 | 13.3916 | 14.88 |
Actin | 0.0281 | 0.0022 | 0.0078 | 0.0055 | 0.0056 | 0.003 | 0.0726 | 0.0019 | 0.0088 | 0.0049 | 0.0165 | 0.0144 | 0.037 | 0.0065 | 0.0317 | 0.025 | 0.0358 | 0.007 |
Bud | 0.0028 | 0.0016 | 0.0018 | 0.0035 | 0.0018 | 0.0012 | 0.0026 | 0.0005 | 0.0017 | 0.0038 | 0.0104 | 0.0008 | 0.0034 | 0.0016 | 0.0051 | 0.0067 | 0.003 | 0.0027 |
Bud Neck | 0.0047 | 0.0006 | 0.0019 | 0.0015 | 0.0019 | 0.0044 | 0.0014 | 0.0003 | 0.0006 | 0.0019 | 0.0012 | 0.0023 | 0.0025 | 0.0006 | 0.0023 | 0.0014 | 0.0025 | 0.0045 |
Bud Periphery | 0.0021 | 0.0007 | 0.0012 | 0.0029 | 0.0005 | 0.0008 | 0.0017 | 0.0001 | 0.0005 | 0.0016 | 0.0026 | 0.0003 | 0.0018 | 0.0005 | 0.0019 | 0.0042 | 0.0013 | 0.0018 |
Bud Site | 0.0142 | 0.002 | 0.0013 | 0.0005 | 0.0008 | 0.0003 | 0.003 | 0.0005 | 0.0007 | 0.0045 | 0.0009 | 0.0002 | 0.0077 | 0.0032 | 0.0105 | 0.0035 | 0.0012 | 0.0005 |
Cell Periphery | 0.0008 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0006 | 0.0003 | 0.0003 | 0.0004 | 0.0007 | 0.0003 |
Cytoplasm | 0.7125 | 0.9285 | 0.8865 | 0.7717 | 0.8844 | 0.8707 | 0.7111 | 0.989 | 0.9171 | 0.8369 | 0.7961 | 0.9127 | 0.7423 | 0.9439 | 0.8358 | 0.8713 | 0.7612 | 0.8559 |
Cytoplasmic Foci | 0.0169 | 0.0151 | 0.0157 | 0.0311 | 0.0165 | 0.0215 | 0.0359 | 0.0035 | 0.009 | 0.0221 | 0.0393 | 0.0245 | 0.0398 | 0.0149 | 0.0288 | 0.0285 | 0.0728 | 0.0304 |
Eisosomes | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0.0005 | 0.0001 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0342 | 0.0039 | 0.0158 | 0.005 | 0.0058 | 0.0159 | 0.0071 | 0.0004 | 0.0021 | 0.0015 | 0.0006 | 0.0077 | 0.0252 | 0.002 | 0.0026 | 0.0037 | 0.0057 | 0.0061 |
Endosome | 0.0542 | 0.016 | 0.0261 | 0.0793 | 0.0308 | 0.039 | 0.0486 | 0.0008 | 0.0322 | 0.0481 | 0.0894 | 0.0237 | 0.0494 | 0.0097 | 0.0191 | 0.0138 | 0.0422 | 0.0377 |
Golgi | 0.008 | 0.0047 | 0.01 | 0.0385 | 0.0138 | 0.0036 | 0.0273 | 0.0003 | 0.0135 | 0.0271 | 0.0299 | 0.0027 | 0.0155 | 0.0073 | 0.0125 | 0.0053 | 0.0135 | 0.0196 |
Lipid Particles | 0.0043 | 0.0002 | 0.0003 | 0.0108 | 0.0078 | 0.0002 | 0.0215 | 0 | 0.0017 | 0.0042 | 0.0015 | 0.0001 | 0.0067 | 0 | 0.0074 | 0.0012 | 0.0163 | 0.0022 |
Mitochondria | 0.0244 | 0.0153 | 0.0114 | 0.0285 | 0.0052 | 0.0114 | 0.0206 | 0.0006 | 0.0027 | 0.0289 | 0.0043 | 0.0027 | 0.0097 | 0.0033 | 0.0184 | 0.0054 | 0.0128 | 0.02 |
None | 0.0048 | 0.0005 | 0.0009 | 0.0006 | 0.0006 | 0.0009 | 0.0036 | 0.0003 | 0.0004 | 0.0005 | 0.0004 | 0.0006 | 0.01 | 0.0005 | 0.002 | 0.0203 | 0.001 | 0.0009 |
Nuclear Periphery | 0.0309 | 0.002 | 0.0055 | 0.002 | 0.0029 | 0.0063 | 0.0044 | 0.0002 | 0.0007 | 0.001 | 0.0004 | 0.0015 | 0.0104 | 0.0009 | 0.001 | 0.0009 | 0.0006 | 0.0011 |
Nucleolus | 0.0004 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0 | 0.0028 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Nucleus | 0.015 | 0.0029 | 0.0044 | 0.0028 | 0.0051 | 0.0063 | 0.0031 | 0.0009 | 0.0018 | 0.0017 | 0.0013 | 0.0025 | 0.0106 | 0.0018 | 0.0025 | 0.0018 | 0.0014 | 0.0025 |
Peroxisomes | 0.0032 | 0.0015 | 0.0012 | 0.0042 | 0.0041 | 0.0006 | 0.019 | 0.0003 | 0.0029 | 0.0037 | 0.0025 | 0.0011 | 0.0047 | 0.0012 | 0.0122 | 0.0022 | 0.025 | 0.0021 |
Punctate Nuclear | 0.0021 | 0.0002 | 0.0004 | 0.0002 | 0.0003 | 0.0005 | 0.0032 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0107 | 0.0001 | 0.0011 | 0.0005 | 0.001 | 0.0003 |
Vacuole | 0.0277 | 0.0016 | 0.0052 | 0.0064 | 0.0083 | 0.0087 | 0.0088 | 0.0002 | 0.0022 | 0.0045 | 0.0015 | 0.0012 | 0.0068 | 0.0013 | 0.0032 | 0.0026 | 0.0009 | 0.0029 |
Vacuole Periphery | 0.0083 | 0.0004 | 0.0021 | 0.0046 | 0.0035 | 0.0043 | 0.0036 | 0.0001 | 0.001 | 0.002 | 0.0009 | 0.0007 | 0.0021 | 0.0004 | 0.0009 | 0.001 | 0.0007 | 0.0015 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 251.4328 | 258.5315 | 234.3605 | 233.1913 | 227.5524 | 198.2893 | 221.0528 | 232.9874 | 255.0202 | 227.6315 |
Translational Efficiency | 1.288 | 1.2527 | 1.4142 | 1.6485 | 1.3525 | 1.5044 | 1.5642 | 1.3444 | 1.4456 | 1.3986 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1958 | 1381 | 307 | 1113 | 1657 | 650 | 1494 | 664 | 3615 | 2031 | 1801 | 1777 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1055.93 | 1377.82 | 1558.24 | 1508.56 | 1391.82 | 1471.91 | 1687.64 | 1492.17 | 1209.89 | 1407.93 | 1665.58 | 1502.44 |
Standard Deviation | 523.18 | 262.06 | 303.11 | 518.51 | 229.22 | 254.76 | 377.04 | 467.55 | 447.60 | 263.43 | 368.72 | 500.14 |
Intensity Change Log 2 | — | 0.383873 | 0.561403 | 0.514658 | — | 0.080717 | 0.278035 | 0.100439 | — | 0.219369 | 0.407153 | 0.293857 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000511 | 0.000386 | 0.000384 | 0.013027 | 0.000187 | 0.000901 | 0.000355 | 0.010433 | 0.000363 | 0.000551 | 0.000360 | 0.012058 |
Bud Neck | 0.002127 | 0.001416 | 0.001776 | 0.006346 | 0.000327 | 0.002104 | 0.001606 | 0.009571 | 0.001302 | 0.001636 | 0.001635 | 0.007551 |
Bud Site | 0.071336 | 0.026932 | 0.004772 | 0.190232* | 0.004905 | 0.071626 | 0.011506 | 0.177483* | 0.040886 | 0.041236 | 0.010358 | 0.185468* |
Cell Periphery | 0.000223 | 0.000108 | 0.000234 | 0.004325 | 0.000081 | 0.000195 | 0.000185 | 0.001571 | 0.000158 | 0.000136 | 0.000193 | 0.003296 |
Cytoplasm | 0.633773* | 0.861295* | 0.775296* | 0.424734* | 0.932505* | 0.717545* | 0.859018* | 0.220776* | 0.770702* | 0.815289* | 0.844746* | 0.348523* |
Cytoplasmic Foci | 0.163745 | 0.036872 | 0.017972 | 0.074961 | 0.011292 | 0.094289 | 0.013562 | 0.090012 | 0.093866 | 0.055247 | 0.014314 | 0.080585 |
Eisosomes | 0.000010 | 0.000003 | 0.000007 | 0.000049 | 0.000001 | 0.000006 | 0.000002 | 0.000059 | 0.000006 | 0.000004 | 0.000003 | 0.000053 |
Endoplasmic Reticulum | 0.002070 | 0.000363 | 0.000453 | 0.001054 | 0.000730 | 0.000614 | 0.000319 | 0.001725 | 0.001456 | 0.000443 | 0.000341 | 0.001305 |
Endosome | 0.011449 | 0.004139 | 0.001698 | 0.025326 | 0.001028 | 0.011806 | 0.001748 | 0.064339 | 0.006672 | 0.006593 | 0.001739 | 0.039904 |
Golgi | 0.033847 | 0.008762 | 0.000904 | 0.074240 | 0.003220 | 0.033913 | 0.003430 | 0.129919* | 0.019809 | 0.016811 | 0.002999 | 0.095045 |
Lipid Particles | 0.000484 | 0.000669 | 0.001427 | 0.003683 | 0.000320 | 0.001231 | 0.001353 | 0.003751 | 0.000409 | 0.000849 | 0.001365 | 0.003708 |
Mitochondria | 0.011109 | 0.000476 | 0.000107 | 0.008244 | 0.000168 | 0.001060 | 0.000092 | 0.019659 | 0.006094 | 0.000663 | 0.000095 | 0.012509 |
Mitotic Spindle | 0.005792 | 0.004442 | 0.004067 | 0.048380 | 0.001299 | 0.011406 | 0.011245 | 0.070846 | 0.003732 | 0.006671 | 0.010021 | 0.056775 |
None | 0.002213 | 0.001697 | 0.005064 | 0.001530 | 0.004221 | 0.000736 | 0.003870 | 0.000823 | 0.003133 | 0.001389 | 0.004074 | 0.001266 |
Nuclear Periphery | 0.000039 | 0.000042 | 0.000288 | 0.000215 | 0.000040 | 0.000049 | 0.000132 | 0.000610 | 0.000040 | 0.000045 | 0.000159 | 0.000363 |
Nuclear Periphery Foci | 0.000594 | 0.001906 | 0.005679 | 0.002255 | 0.001863 | 0.001952 | 0.002551 | 0.003654 | 0.001176 | 0.001921 | 0.003084 | 0.002778 |
Nucleolus | 0.000218 | 0.000389 | 0.000114 | 0.000637 | 0.000110 | 0.000292 | 0.000104 | 0.000696 | 0.000169 | 0.000358 | 0.000106 | 0.000659 |
Nucleus | 0.009180 | 0.017616 | 0.129402 | 0.011547 | 0.025231 | 0.008817 | 0.064763 | 0.009483 | 0.016537 | 0.014800 | 0.075782 | 0.010776 |
Peroxisomes | 0.001442 | 0.000800 | 0.000080 | 0.008749 | 0.000090 | 0.002759 | 0.000195 | 0.013796 | 0.000822 | 0.001427 | 0.000175 | 0.010635 |
Vacuole | 0.021118 | 0.025560 | 0.050116 | 0.079438 | 0.009509 | 0.028702 | 0.023174 | 0.138810 | 0.015797 | 0.026565 | 0.027766 | 0.101623 |
Vacuole Periphery | 0.028721 | 0.006127 | 0.000161 | 0.021026 | 0.002871 | 0.009997 | 0.000789 | 0.031984 | 0.016872 | 0.007365 | 0.000682 | 0.025120 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.78 | -1.07 | -5.97 | -6.04 | -5.90 | -6.55 | -3.84 | -5.09 | -4.66 | -4.95 | -2.51 | -1.39 | -7.82 | -7.64 | -7.74 |
Bud Neck | 2.25 | -3.36 | -11.32 | -14.10 | -6.03 | -7.97 | -11.32 | -8.97 | -7.07 | -7.20 | -2.87 | -6.17 | -13.93 | -12.97 | -11.32 |
Bud Site | 13.43 | 22.66* | -12.68 | -19.95 | -23.72* | -13.39 | -4.48 | -19.66 | -9.88 | -18.89 | -0.18 | 16.83 | -22.94 | -22.17 | -29.80* |
Cell Periphery | 6.29 | -3.23 | -3.53 | -3.67 | -3.40 | -3.88 | -6.74 | -5.44 | -4.93 | -4.90 | 1.44 | -4.07 | -4.40 | -4.44 | -4.26 |
Cytoplasm | -23.95 | -2.79 | 13.10 | 33.62 | 12.44 | 17.01 | 21.13 | 57.30* | 27.61 | 45.14* | -5.50 | -2.50 | 40.45 | 43.17 | 42.91 |
Cytoplasmic Foci | 25.52 | 29.89 | 17.73 | -7.53 | -12.07 | -15.12 | -4.12 | -16.56 | 0.97 | -15.50 | 10.61 | 26.82 | 5.11 | -5.40 | -20.73 |
Eisosomes | 9.60 | -1.58 | -6.44 | -7.87 | -5.89 | -5.28 | -4.84 | -8.06 | -7.24 | -7.75 | 3.08 | 2.14 | -10.32 | -10.74 | -10.59 |
Endoplasmic Reticulum | 6.69 | 5.11 | 4.55 | -3.68 | -0.90 | 0.42 | 1.60 | -2.23 | -3.08 | -4.23 | 5.30 | 5.50 | 1.43 | -4.73 | -4.94 |
Endosome | 8.15 | 10.57 | -5.53 | -11.27 | -12.74 | -9.71 | -2.30 | -13.80 | -10.75 | -13.66 | 0.18 | 10.74 | -14.81 | -14.78 | -17.98 |
Golgi | 13.42 | 20.23 | -4.35 | -10.37 | -12.43 | -9.35 | 1.09 | -13.22 | -8.92 | -13.34 | 2.29 | 16.25 | -13.12 | -13.76 | -17.74 |
Lipid Particles | -3.23 | -4.39 | -7.40 | -6.90 | -2.35 | -4.97 | -9.19 | -4.99 | -3.45 | -2.58 | -6.07 | -10.05 | -8.92 | -7.61 | -4.57 |
Mitochondria | 9.36 | 9.70 | 3.31 | -5.33 | -5.68 | -3.06 | 1.63 | -5.67 | -5.35 | -5.69 | 8.63 | 9.68 | -2.74 | -7.33 | -7.79 |
Mitotic Spindle | 0.94 | -0.11 | -10.30 | -10.42 | -9.67 | -4.87 | -6.48 | -10.74 | -8.60 | -8.87 | -3.52 | -5.15 | -14.95 | -13.60 | -12.15 |
None | 1.12 | -2.73 | 1.23 | 0.48 | 4.43 | 8.35 | 2.13 | 7.69 | -0.85 | 6.82 | 5.27 | -0.34 | 4.68 | 0.44 | 5.75 |
Nuclear Periphery | -0.85 | -11.78 | -6.52 | -6.29 | 5.87 | -0.97 | -14.99 | -3.47 | -3.38 | -2.27 | -1.24 | -19.26 | -5.46 | -5.31 | -1.42 |
Nuclear Periphery Foci | -6.16 | -9.14 | -4.26 | -0.86 | 5.97 | -0.67 | -4.55 | -2.15 | -1.85 | -0.57 | -4.52 | -11.19 | -4.17 | -1.93 | 2.26 |
Nucleolus | -1.23 | 1.10 | -1.55 | -0.93 | -1.74 | -1.71 | -1.17 | -3.02 | -1.88 | -2.92 | -1.95 | 1.15 | -2.66 | -1.59 | -2.83 |
Nucleus | -5.28 | -17.58 | -5.35 | -0.73 | 16.07 | 8.48 | -19.62 | 5.67 | -1.54 | 22.00 | 0.58 | -28.20 | -0.60 | -1.11 | 26.64 |
Peroxisomes | 3.45 | 8.79 | -6.65 | -7.46 | -8.36 | -5.95 | -3.38 | -7.80 | -5.86 | -7.74 | -3.47 | 7.67 | -10.38 | -9.44 | -11.26 |
Vacuole | -2.91 | -9.18 | -16.70 | -14.85 | 0.67 | -6.92 | -15.09 | -16.92 | -13.85 | -10.62 | -7.26 | -16.39 | -23.81 | -20.19 | -10.22 |
Vacuole Periphery | 11.38 | 16.00 | 5.28 | -4.98 | -8.94 | -4.50 | 4.46 | -9.15 | -5.90 | -10.18 | 7.63 | 16.04 | -1.77 | -7.78 | -13.28 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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