Standard name
Human Ortholog
Description Integral membrane protein of the ER; forms an ER-membrane associated protein complex with Slp1p; identified along with SLP1 in a screen for mutants defective in the unfolded protein response (UPR); proposed to function in the folding of integral membrane proteins; interacts genetically with MPS3; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.28 0.26 – 0.22 0.16 0.08 0.09 0.12 0.09 0.05 0 0.06 0.14 0.08 0 0.44 0.31 0.33 0.44 0.32 0.25 0.37 0.25 0.25
Bud 0.06 0.05 – 0.05 0 0 0 0.07 0.05 0.06 0.08 0.05 0 0 0 0.08 0.06 0.07 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.1 0.05 0 0.06 0.07
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.06 0.09 – 0.12 0.09 0.08 0 0 0 0 0 0 0.1 0 0.05 0.15 0.16 0.09 0 0.06 0.08 0.05 0.08 0.08
Cytoplasm 0.26 0.43 – 0.54 0.59 0.59 0.53 0.51 0.38 0.45 0.34 0.41 0.69 0.89 0.95 0.25 0.35 0.33 0 0.05 0 0.06 0.11 0.13
Endoplasmic Reticulum 0.45 0.32 – 0.16 0.18 0.17 0.19 0.15 0.21 0.07 0.05 0.07 0.14 0.05 0 0.06 0.11 0.1 0.18 0.18 0.07 0.13 0.12 0.13
Endosome 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0 0
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.2 0.06 – 0 0 0.14 0.12 0.18 0.36 0.39 0.66 0.47 0 0 0 0.09 0.07 0.07 0.17 0.16 0.31 0.21 0.22 0.2
Nucleus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.06 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0.07 0.07 0.12 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.09 0 0.06 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 34 31 – 37 33 17 27 37 18 14 6 16 25 23 7 76 85 97 75 50 14 20 57 73
Bud 7 6 – 9 4 6 11 21 9 18 21 13 4 4 0 14 17 22 0 3 1 0 3 10
Bud Neck 1 1 – 4 2 1 4 11 1 2 0 2 3 2 3 0 11 0 7 15 3 2 14 20
Bud Site 0 1 – 0 0 1 2 1 2 1 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 7 11 – 20 18 16 13 6 4 11 2 5 17 6 20 26 43 27 5 8 4 2 17 23
Cytoplasm 31 51 – 92 123 124 158 157 74 131 88 105 120 263 409 44 95 99 6 7 1 3 23 39
Endoplasmic Reticulum 54 38 – 27 37 35 58 47 40 20 13 19 25 16 5 11 30 31 30 27 4 7 26 36
Endosome 0 0 – 2 2 6 10 5 1 5 4 3 0 0 1 2 9 14 3 1 3 2 6 4
Golgi 0 0 – 1 0 1 1 0 0 0 0 0 0 0 1 2 1 1 2 3 0 0 0 2
Mitochondria 24 7 – 4 1 29 37 57 70 114 170 120 0 0 2 15 18 21 28 24 17 11 50 58
Nucleus 0 0 – 0 0 1 0 1 0 3 0 1 0 0 0 2 3 1 0 0 0 0 2 0
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 1 2 2 0 0 6 1 3 0 5 4 8 17 19 1 0 0 0 1 0
SpindlePole 0 4 – 5 9 7 8 14 8 17 3 7 0 4 2 12 20 35 0 0 0 0 0 2
Vac/Vac Membrane 1 2 – 0 3 5 5 6 3 8 3 10 0 0 2 2 5 6 6 11 5 1 14 17
Unique Cell Count 121 119 171 208 210 300 309 194 292 256 257 173 297 430 173 273 298 169 155 58 55 226 294
Labelled Cell Count 159 152 201 233 251 336 363 230 351 313 304 194 323 457 214 355 373 169 155 58 55 226 294


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 5.6 – 6.0 6.2 5.2 5.1 5.1 4.4 4.5 4.0 4.3 6.1 6.3 6.1 6.8 6.5 6.4 – 6.2 6.5
Std Deviation (1e-4) 0.5 0.9 – 1.1 1.5 1.3 1.2 1.0 0.9 0.9 0.9 0.9 1.1 1.4 1.3 1.0 1.0 1.1 – 1.6 1.7
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.1463 1.1865 1.2029 1.2184 0.5267 1.2744 0.319 0.8554 0.5319 0.7522 0.4786 0.8878 -0.3995 -0.2167 -0.4491 -0.1236 -0.2046 -0.3585
Actin 0.095 0.0675 0.108 0.0784 0.1485 0.0888 0.1045 0.0946 0.065 0.1853 0.1017 0.0659 0.1055 0.0881 0.1205 0.1214 0.1468 0.0997
Bud 0.0023 0.0042 0.0068 0.0182 0.0045 0.0011 0.0016 0.0009 0.0065 0.0016 0.0006 0.0012 0.0008 0.0021 0.0051 0.0163 0.0031 0.0012
Bud Neck 0.0125 0.0043 0.0097 0.0329 0.0545 0.0662 0.0116 0.0092 0.0128 0.0251 0.0206 0.0728 0.0127 0.0088 0.0094 0.0074 0.0049 0.1194
Bud Periphery 0.0084 0.0107 0.0262 0.045 0.0246 0.0037 0.0047 0.0018 0.0398 0.0108 0.004 0.0055 0.0018 0.007 0.0128 0.0769 0.0069 0.0044
Bud Site 0.0301 0.0999 0.0375 0.0161 0.0083 0.003 0.0239 0.1098 0.0193 0.0028 0.0017 0.0063 0.0237 0.0968 0.0474 0.0067 0.0077 0.0028
Cell Periphery 0.0222 0.0106 0.0132 0.0104 0.0233 0.0051 0.0229 0.0236 0.0231 0.0212 0.0185 0.0218 0.0171 0.0218 0.0165 0.0097 0.009 0.0116
Cytoplasm 0.3632 0.3676 0.309 0.3096 0.2768 0.382 0.2524 0.3313 0.3751 0.2988 0.4741 0.3779 0.2751 0.3797 0.322 0.3582 0.301 0.39
Cytoplasmic Foci 0.0763 0.0682 0.0559 0.0827 0.0478 0.0817 0.105 0.0485 0.0666 0.0629 0.0745 0.08 0.0871 0.0526 0.0835 0.0703 0.0655 0.0661
Eisosomes 0.009 0.0035 0.0042 0.0012 0.0041 0.0013 0.0074 0.0035 0.0035 0.0074 0.0026 0.0016 0.0032 0.0052 0.0047 0.001 0.0038 0.0011
Endoplasmic Reticulum 0.0394 0.0398 0.0413 0.0435 0.0701 0.0372 0.0261 0.0359 0.0597 0.0272 0.0274 0.0756 0.0532 0.0212 0.0312 0.0327 0.0263 0.0367
Endosome 0.0464 0.0418 0.0339 0.0968 0.0243 0.0368 0.0489 0.0173 0.0223 0.024 0.0192 0.027 0.0602 0.0209 0.0378 0.0299 0.0674 0.0396
Golgi 0.0287 0.0274 0.0278 0.0346 0.0146 0.0229 0.0386 0.0117 0.0241 0.0237 0.0225 0.0249 0.0396 0.0221 0.036 0.0464 0.0971 0.0208
Lipid Particles 0.0315 0.0144 0.0144 0.0211 0.0085 0.014 0.0644 0.0319 0.0351 0.0273 0.0685 0.0255 0.0608 0.0164 0.0367 0.0733 0.0342 0.0102
Mitochondria 0.0178 0.0114 0.0207 0.0315 0.0106 0.0132 0.0231 0.0023 0.0139 0.0095 0.0032 0.0211 0.0124 0.0019 0.0139 0.0201 0.034 0.008
None 0.1826 0.2065 0.2536 0.1368 0.2339 0.2091 0.2364 0.2654 0.2032 0.2267 0.1498 0.1648 0.2015 0.2421 0.1871 0.0879 0.131 0.1668
Nuclear Periphery 0.0037 0.0024 0.0031 0.0064 0.004 0.0061 0.0022 0.0015 0.0032 0.002 0.0005 0.0019 0.0066 0.0012 0.0034 0.0009 0.0206 0.0061
Nucleolus 0.0015 0.001 0.0015 0.0007 0.0024 0.0011 0.001 0.0004 0.001 0.0004 0.0005 0.0004 0.0006 0.0004 0.0006 0.0002 0.0017 0.0003
Nucleus 0.0032 0.0032 0.0042 0.0042 0.0136 0.0045 0.0011 0.0011 0.0018 0.0011 0.0007 0.0013 0.0015 0.0009 0.0012 0.0008 0.0084 0.0035
Peroxisomes 0.0124 0.0063 0.0148 0.0173 0.0057 0.0098 0.0125 0.0029 0.0099 0.0331 0.0032 0.01 0.0219 0.0062 0.0199 0.0325 0.0106 0.0046
Punctate Nuclear 0.0049 0.0027 0.0086 0.0035 0.0131 0.0077 0.0054 0.0011 0.0088 0.0047 0.0008 0.0074 0.0028 0.0011 0.0027 0.0015 0.0123 0.0023
Vacuole 0.0079 0.0059 0.0048 0.0074 0.0058 0.0041 0.0048 0.0051 0.0043 0.0035 0.0047 0.0061 0.0103 0.0034 0.0066 0.005 0.0047 0.0038
Vacuole Periphery 0.0011 0.0007 0.0009 0.0018 0.0011 0.0007 0.0015 0.0003 0.0008 0.0009 0.0004 0.0009 0.0016 0.0002 0.0011 0.0006 0.0031 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.216 14.167 12.2646 14.1489 8.1333 5.9396 12.3845 14.5018 14.2986 13.3053
Translational Efficiency 0.4539 0.3149 0.6819 0.5198 0.5781 1.0325 0.5916 0.4357 0.5086 0.4479

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1381 608 470 488 1746 1450 173 169 3127 2058 643 657

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 560.09 716.28 988.57 959.80 725.37 761.44 983.87 938.31 652.38 748.10 987.31 954.27
Standard Deviation 63.05 119.42 116.45 129.85 84.02 92.54 102.15 108.54 111.51 103.30 112.80 125.07
Intensity Change Log 2 — 0.354865 0.819684 0.777075 — 0.070013 0.439751 0.371348 — 0.201088 0.617697 0.562279

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.011901 0.034287 0.022789 0.099717 0.024442 0.042314 0.052273 0.053388 0.018904 0.039942 0.030722 0.087800
Bud Neck 0.031515 0.040202 0.015338 0.030616 0.029106 0.063607 0.017994 0.023938 0.030170 0.056692 0.016052 0.028898
Bud Site 0.015652 0.031652 0.058400 0.108370* 0.019795 0.042838 0.065661 0.058516 0.017965 0.039533 0.060353 0.095546
Cell Periphery 0.006999 0.006210 0.003918 0.019855 0.007789 0.010451 0.012285 0.020156 0.007440 0.009198 0.006169 0.019932
Cytoplasm 0.299787* 0.171035* 0.316223* 0.238420* 0.359788* 0.331190* 0.223666* 0.222258* 0.333289* 0.283875* 0.291320* 0.234262*
Cytoplasmic Foci 0.198856* 0.246490* 0.117689 0.084449 0.192153* 0.140693 0.145368 0.131553 0.195113* 0.171949 0.125136 0.096565
Eisosomes 0.023316 0.013569 0.001605 0.027319 0.035004 0.041021 0.009372 0.022531 0.029842 0.032911 0.003695 0.026087
Endoplasmic Reticulum 0.011367 0.021977 0.074288 0.058733 0.023708 0.017155 0.092453 0.050580 0.018258 0.018579 0.079175 0.056636
Endosome 0.010189 0.062411 0.011888 0.007511 0.011874 0.026729 0.015706 0.015549 0.011130 0.037271 0.012915 0.009579
Golgi 0.065705 0.101174 0.002062 0.007893 0.078002 0.077381 0.005564 0.003828 0.072571 0.084410 0.003004 0.006847
Lipid Particles 0.089260 0.101113* 0.019782 0.030967 0.070581 0.050344 0.039587 0.044838 0.078830 0.065343 0.025110 0.034535
Mitochondria 0.090208 0.041453 0.000436 0.002857 0.025539 0.042603 0.000272 0.000504 0.054099 0.042264 0.000392 0.002252
Mitotic Spindle 0.000225 0.003294 0.002574 0.003662 0.000164 0.001411 0.000577 0.001525 0.000191 0.001967 0.002036 0.003112
None 0.006664 0.000663 0.000435 0.000491 0.001646 0.000689 0.000192 0.000117 0.003862 0.000681 0.000370 0.000395
Nuclear Periphery 0.000227 0.000365 0.001021 0.000364 0.000174 0.000102 0.000742 0.000459 0.000198 0.000180 0.000946 0.000388
Nuclear Periphery Foci 0.000383 0.001098 0.021322 0.006841 0.000525 0.000464 0.016320 0.010114 0.000462 0.000652 0.019976 0.007683
Nucleolus 0.000373 0.000419 0.000174 0.000091 0.000301 0.000351 0.000150 0.000147 0.000333 0.000371 0.000167 0.000105
Nucleus 0.032048 0.013182 0.151246* 0.067829 0.040824 0.021460 0.098812 0.072512 0.036948 0.019015 0.137139* 0.069034
Peroxisomes 0.095160 0.074849 0.003518 0.012767 0.063426 0.063353 0.014026 0.011712 0.077441 0.066750 0.006345 0.012496
Vacuole 0.007974 0.028565 0.175010 0.188853 0.013727 0.023089 0.188763 0.255472* 0.011186 0.024707 0.178711 0.205989*
Vacuole Periphery 0.002192 0.005993 0.000282 0.002397 0.001434 0.002753 0.000218 0.000303 0.001769 0.003710 0.000265 0.001859

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.59 -3.05 -10.75 -6.86 -8.73 -6.00 -3.13 -2.83 -0.59 0.12 -9.01 -3.13 -10.28 -6.40 -7.70
Bud Neck -2.01 6.16 0.95 2.58 -4.17 -9.34 2.87 1.66 7.68 -0.67 -9.22 6.95 1.40 8.89 -3.82
Bud Site -4.63 -9.34 -11.35 -8.81 -5.12 -8.86 -5.48 -4.09 -1.32 0.80 -10.34 -10.68 -11.83 -8.10 -4.30
Cell Periphery 0.56 4.45 -5.18 -4.93 -7.02 -2.67 -1.46 -2.15 -1.53 -1.18 -2.16 1.97 -5.38 -4.30 -6.02
Cytoplasm 13.46 0.11 6.04 -5.20 4.78 3.61 8.72 9.25 7.34 0.10 7.74 6.01 11.90 6.01 4.13
Cytoplasmic Foci -5.74 12.38 16.20 18.21 3.85 9.44 5.21 5.82 1.45 1.18 5.02 13.64 17.29 12.66 3.89
Eisosomes 4.96 15.15 -0.32 -3.76 -9.07 -2.24 9.99 3.97 5.34 -2.60 -1.61 20.19 2.90 3.91 -9.10
Endoplasmic Reticulum -3.69 -11.60 -8.59 -5.87 2.51 3.09 -6.45 -3.53 -4.52 3.40 -0.20 -12.38 -8.33 -8.13 3.89
Endosome -13.43 -1.93 3.25 14.11 4.68 -9.65 -2.07 -0.71 4.30 0.49 -16.31 -2.14 2.25 16.08 3.60
Golgi -6.11 26.99 20.94 17.17 -3.25 0.16 29.18 30.19 25.69 1.62 -3.90 40.99 32.67 28.34 -2.72
Lipid Particles -1.63 19.42 13.90 10.17 -3.32 6.10 6.77 3.93 1.13 -0.53 4.18 21.47 13.72 8.88 -2.70
Mitochondria 8.92 21.27 19.19 9.99 -1.19 -5.92 18.31 18.14 16.62 -1.56 4.02 25.61 21.12 16.53 -1.22
Mitotic Spindle -1.93 -2.58 -2.49 -0.40 -0.57 -2.28 -4.08 -2.07 0.07 -1.35 -2.92 -2.74 -2.74 -1.12 -0.74
None 4.87 5.13 5.04 0.82 -0.24 3.60 5.86 6.27 5.45 1.97 5.64 6.27 6.10 1.93 -0.10
Nuclear Periphery -1.46 -8.56 -2.01 -0.21 6.04 3.38 -7.89 -3.96 -4.98 2.77 0.55 -10.77 -3.59 -3.71 6.58
Nuclear Periphery Foci -4.04 -14.75 -11.67 -10.41 9.34 0.58 -11.76 -6.58 -6.61 3.26 -1.92 -17.71 -13.16 -12.76 9.86
Nucleolus -0.46 3.46 5.12 4.45 4.76 -0.27 1.90 1.99 1.59 0.42 -0.30 3.22 4.59 2.86 4.23
Nucleus 9.32 -16.48 -8.04 -12.56 10.23 9.61 -6.28 -4.48 -7.23 2.46 12.27 -16.99 -8.57 -13.61 10.10
Peroxisomes 3.76 25.93 21.81 14.43 -5.57 0.02 13.79 19.46 18.25 0.77 3.73 33.11 28.58 23.24 -3.78
Vacuole -8.49 -22.43 -19.72 -17.47 -1.94 -6.32 -13.47 -14.34 -13.85 -3.51 -10.79 -25.95 -23.96 -22.44 -3.47
Vacuole Periphery -5.16 10.33 0.08 2.59 -1.36 -6.19 10.81 7.37 11.63 -0.40 -7.22 14.38 0.16 2.06 -1.37
Standard name
Human Ortholog
Description Integral membrane protein of the ER; forms an ER-membrane associated protein complex with Slp1p; identified along with SLP1 in a screen for mutants defective in the unfolded protein response (UPR); proposed to function in the folding of integral membrane proteins; interacts genetically with MPS3; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
Localization
Cell Percentages ER (47%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Emp65

Emp65


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Emp65-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available