Standard name | |
---|---|
Human Ortholog | |
Description | 3-keto sterol reductase; catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs; mutation is functionally complemented by human HSD17B7 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 19 | 12 | 26 | 32 | 56 | 28 | 17 | 22 | 9 | 5 | 2 | 3 | 15 | 7 | 3 | 34 | 14 | 40 | 50 | 25 | 27 | 16 | 29 | 21 |
Bud | 0 | 1 | 2 | 4 | 4 | 3 | 5 | 4 | 4 | 4 | 0 | 5 | 0 | 1 | 0 | 2 | 1 | 4 | 0 | 0 | 0 | 0 | 2 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 1 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 7 | 0 | 2 | 9 | 6 | 17 | 13 | 7 | 10 | 4 | 5 | 7 | 2 | 7 | 1 | 20 | 7 | 13 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 1 | 5 | 7 | 0 | 32 | 15 | 8 | 0 | 2 | 1 | 0 | 0 | 1 | 4 | 2 | 10 | 6 | 14 | 9 | 1 | 10 | 2 | 5 | 1 |
Endosome | 26 | 0 | 6 | 12 | 20 | 15 | 30 | 10 | 1 | 4 | 0 | 11 | 11 | 9 | 13 | 8 | 3 | 5 | 46 | 31 | 28 | 18 | 28 | 24 |
Golgi | 17 | 8 | 56 | 64 | 145 | 90 | 44 | 26 | 5 | 10 | 0 | 3 | 33 | 36 | 60 | 303 | 106 | 206 | 72 | 36 | 43 | 34 | 43 | 46 |
Mitochondria | 177 | 72 | 51 | 91 | 53 | 122 | 323 | 229 | 259 | 308 | 300 | 312 | 44 | 14 | 16 | 39 | 12 | 26 | 6 | 5 | 3 | 4 | 7 | 9 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 2 | 4 | 2 | 0 | 1 | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 13 | 8 | 7 | 10 | 5 | 20 | 27 | 22 | 21 | 27 | 27 | 29 | 4 | 12 | 8 | 11 | 1 | 7 | 2 | 4 | 3 | 2 | 4 | 2 |
Nucleolus | 57 | 19 | 15 | 30 | 19 | 42 | 93 | 86 | 87 | 96 | 94 | 93 | 42 | 64 | 70 | 65 | 29 | 65 | 1 | 1 | 0 | 1 | 0 | 0 |
Peroxisomes | 19 | 2 | 11 | 16 | 2 | 0 | 6 | 3 | 5 | 8 | 0 | 4 | 9 | 4 | 6 | 2 | 0 | 4 | 62 | 11 | 30 | 31 | 56 | 55 |
SpindlePole | 6 | 0 | 6 | 13 | 7 | 5 | 4 | 4 | 0 | 4 | 0 | 3 | 13 | 15 | 16 | 13 | 3 | 8 | 8 | 0 | 0 | 1 | 4 | 6 |
Vac/Vac Membrane | 3 | 2 | 5 | 3 | 10 | 19 | 8 | 5 | 18 | 29 | 11 | 24 | 2 | 2 | 4 | 30 | 2 | 12 | 4 | 6 | 3 | 3 | 6 | 5 |
Unique Cell Count | 241 | 94 | 119 | 191 | 229 | 259 | 442 | 327 | 323 | 405 | 358 | 398 | 127 | 129 | 155 | 410 | 141 | 313 | 267 | 127 | 154 | 120 | 195 | 179 |
Labelled Cell Count | 345 | 130 | 195 | 285 | 361 | 376 | 581 | 421 | 423 | 505 | 444 | 497 | 176 | 177 | 199 | 541 | 186 | 405 | 267 | 127 | 154 | 120 | 195 | 179 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 13.7 | 17.9 | 15.0 | 14.7 | 15.8 | 14.8 | 14.5 | 15.6 | 13.9 | 14.7 | 13.5 | 14.1 | 17.8 | 18.8 | 18.8 | 21.8 | 24.7 | 23.6 | 14.7 | 15.4 | 15.8 |
Std Deviation (1e-4) | 2.8 | 4.4 | 2.6 | 2.3 | 2.5 | 2.6 | 2.8 | 3.2 | 3.3 | 3.4 | 3.1 | 3.3 | 3.7 | 4.0 | 3.1 | 5.4 | 6.1 | 6.1 | 2.8 | 3.5 | 3.4 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.08 | -0.02 | -0.05 | 0.05 | -0.12 | -0.04 | -0.16 | -0.09 | 0.25 | 0.33 | 0.32 | 0.54 | 0.72 | 0.65 | -0.03 | 0.04 | 0.08 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.1 | 0.5 | -2.8 | -6.6 | -4.6 | -6.6 | -8.4 | -8.6 | -8.8 | -2.1 | -3.8 | -5.3 | -4.1 | -2.7 | -2.3 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 2.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.1 | 0 | 0 | -0.6 | -0.6 |
Endosome | 0.5 | 1.2 | 0.3 | 0.7 | 0 | 0 | 0 | 0 | 0 | 1.1 | 0.6 | 1.1 | 0 | 0 | 0 |
Golgi | -2.4 | 2.9 | -2.3 | -9.4 | -9.4 | -12.3 | -12.9 | -13.8 | -13.9 | -3.4 | -3.1 | -1.4 | 5.5 | 4.7 | 3.6 |
Mitochondria | 0.8 | -3.8 | 0.8 | 6.2 | 5.3 | 7.6 | 6.9 | 8.8 | 7.4 | -1.3 | -5.7 | -6.2 | -8.5 | -6.4 | -8.4 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -0.2 | 0 | 0.6 | 0.1 | 0.3 | 0.2 | 0.3 | 0.6 | 0.5 | -1.0 | 1.0 | -0.3 | 0 | 0 | 0 |
Nucleolus | 0.8 | -1.3 | 0.9 | 2.1 | 3.1 | 3.2 | 2.6 | 3.1 | 2.5 | 3.8 | 6.2 | 5.8 | 0.9 | 1.7 | 2.0 |
Peroxisomes | -0.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.6 | -2.0 | -1.8 | 0 | -3.7 | 0 |
SpindlePole | 0.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.5 | 1.9 | 1.6 | 0 | 0 | 0 |
Vacuole | 0 | 0.1 | 1.2 | 0 | 0 | 0.6 | 1.2 | 0 | 0.8 | 0 | 0 | 0 | 1.2 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.119 | 10.3679 | 9.3564 | 8.0656 | 8.4028 | 9.6533 | 7.8502 | 9.6932 | 8.7427 | 8.8421 | 7.7909 | 9.0553 | 7.3571 | 10.7697 | 9.1182 | 7.7607 | 7.3324 | 10.3102 |
Actin | 0.0277 | 0.0027 | 0.0083 | 0.0039 | 0.0011 | 0.0132 | 0.0049 | 0.0054 | 0.0111 | 0.0006 | 0.0008 | 0.0008 | 0.0562 | 0.0016 | 0.0177 | 0.0282 | 0.0096 | 0.0243 |
Bud | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0005 | 0 | 0.0003 | 0.0002 | 0.0001 | 0 |
Bud Neck | 0.0007 | 0.0004 | 0.0013 | 0.0004 | 0.0001 | 0.0005 | 0.0007 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0032 | 0.0001 | 0.0015 | 0.0015 | 0.0003 | 0.0002 |
Bud Periphery | 0.0006 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0006 | 0 | 0.0005 | 0.0003 | 0.0002 | 0 |
Bud Site | 0.0039 | 0.0013 | 0.0047 | 0.0006 | 0.0003 | 0.0003 | 0.0016 | 0.0001 | 0.0012 | 0.0001 | 0.0001 | 0.0001 | 0.0098 | 0.0001 | 0.0066 | 0.0008 | 0.0002 | 0.0001 |
Cell Periphery | 0.0004 | 0.0005 | 0.0005 | 0.0002 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0005 | 0.0003 | 0.0003 | 0.0002 | 0.0002 | 0.0001 |
Cytoplasm | 0.013 | 0.0006 | 0.0009 | 0.0003 | 0.0001 | 0.0008 | 0.0009 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0174 | 0.0001 | 0.0004 | 0.0008 | 0.0066 | 0 |
Cytoplasmic Foci | 0.0674 | 0.0667 | 0.0587 | 0.047 | 0.0562 | 0.0308 | 0.0537 | 0.0433 | 0.0536 | 0.0589 | 0.0505 | 0.026 | 0.0629 | 0.0474 | 0.0576 | 0.0374 | 0.0258 | 0.027 |
Eisosomes | 0.0006 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 | 0.0002 | 0.0003 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0089 | 0.0007 | 0.0012 | 0.0009 | 0.0001 | 0.0024 | 0.001 | 0.0001 | 0.0003 | 0.0003 | 0 | 0.0003 | 0.0015 | 0.0004 | 0.0014 | 0.0038 | 0.0093 | 0.0002 |
Endosome | 0.06 | 0.0363 | 0.031 | 0.0373 | 0.0083 | 0.0206 | 0.0144 | 0.0152 | 0.0221 | 0.0388 | 0.0165 | 0.0075 | 0.0137 | 0.0126 | 0.022 | 0.0396 | 0.0429 | 0.0043 |
Golgi | 0.0505 | 0.0551 | 0.0647 | 0.041 | 0.0283 | 0.0409 | 0.0273 | 0.03 | 0.0615 | 0.0866 | 0.0542 | 0.0301 | 0.0271 | 0.0458 | 0.0491 | 0.0951 | 0.0585 | 0.0279 |
Lipid Particles | 0.6384 | 0.7168 | 0.7257 | 0.7703 | 0.8188 | 0.8117 | 0.801 | 0.8558 | 0.7661 | 0.7244 | 0.7859 | 0.8922 | 0.6922 | 0.8571 | 0.7603 | 0.7408 | 0.7131 | 0.8628 |
Mitochondria | 0.0363 | 0.0126 | 0.013 | 0.0471 | 0.0113 | 0.0428 | 0.0128 | 0.0064 | 0.0193 | 0.0089 | 0.0109 | 0.0064 | 0.0315 | 0.0023 | 0.0193 | 0.0143 | 0.0802 | 0.0049 |
None | 0.0078 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0004 | 0.0033 | 0 | 0.0002 | 0 | 0 | 0 | 0.0167 | 0 | 0.0009 | 0.0007 | 0.0108 | 0 |
Nuclear Periphery | 0.0108 | 0.0156 | 0.0027 | 0.0009 | 0.0004 | 0.0048 | 0.0051 | 0.001 | 0.0035 | 0.0004 | 0.0001 | 0.0024 | 0.0032 | 0.0004 | 0.0022 | 0.0052 | 0.0199 | 0.0006 |
Nucleolus | 0.0146 | 0.0266 | 0.0308 | 0.0003 | 0.001 | 0.003 | 0.0291 | 0.0133 | 0.0007 | 0.0007 | 0.0004 | 0.006 | 0.0104 | 0.0018 | 0.0012 | 0.0005 | 0.0007 | 0.0165 |
Nucleus | 0.0112 | 0.0026 | 0.0022 | 0.0002 | 0.0002 | 0.001 | 0.002 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0038 | 0.0001 | 0.0005 | 0.0005 | 0.0049 | 0.0006 |
Peroxisomes | 0.025 | 0.0222 | 0.0388 | 0.0442 | 0.0359 | 0.0171 | 0.0255 | 0.0232 | 0.0539 | 0.0726 | 0.0781 | 0.022 | 0.023 | 0.018 | 0.0442 | 0.0229 | 0.0096 | 0.0174 |
Punctate Nuclear | 0.0138 | 0.0242 | 0.0036 | 0.0011 | 0.0363 | 0.0034 | 0.0119 | 0.0008 | 0.0011 | 0.0007 | 0.0005 | 0.0014 | 0.0209 | 0.0073 | 0.0058 | 0.0031 | 0.0017 | 0.0109 |
Vacuole | 0.0032 | 0.0071 | 0.0064 | 0.0023 | 0.0003 | 0.002 | 0.0013 | 0.0013 | 0.0013 | 0.0024 | 0.0005 | 0.0008 | 0.0013 | 0.001 | 0.0026 | 0.0013 | 0.0017 | 0.0004 |
Vacuole Periphery | 0.0048 | 0.007 | 0.0044 | 0.0015 | 0.0009 | 0.0037 | 0.003 | 0.0031 | 0.0029 | 0.0036 | 0.0012 | 0.0035 | 0.0034 | 0.0034 | 0.0053 | 0.0026 | 0.0037 | 0.0017 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 96.178 | 102.0389 | 114.9781 | 122.822 | 92.4965 | 98.2374 | 135.1737 | 137.3333 | 142.364 | 134.7439 |
Translational Efficiency | 1.3385 | 1.347 | 1.35 | 1.2721 | 1.1229 | 1.4371 | 1.2992 | 1.3859 | 1.163 | 1.0922 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
933 | 307 | 798 | 1990 | 1714 | 2225 | 1892 | 1286 | 2647 | 2532 | 2690 | 3276 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1424.93 | 1657.65 | 1841.88 | 1698.89 | 1469.52 | 1508.29 | 1661.24 | 1667.64 | 1453.80 | 1526.40 | 1714.83 | 1686.62 |
Standard Deviation | 230.89 | 291.30 | 313.40 | 360.98 | 270.28 | 253.25 | 319.94 | 363.20 | 257.97 | 262.72 | 328.55 | 362.17 |
Intensity Change Log 2 | — | 0.218248 | 0.370288 | 0.253701 | — | 0.037569 | 0.176916 | 0.182463 | — | 0.129345 | 0.275351 | 0.217973 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.016884 | 0.012082 | 0.079933 | 0.084518 | 0.019068 | 0.012080 | 0.055417 | 0.083951 | 0.018299 | 0.012080 | 0.062690 | 0.084295 |
Bud Neck | 0.001881 | 0.002922 | 0.001141 | 0.004320 | 0.000900 | 0.000950 | 0.002736 | 0.004572 | 0.001246 | 0.001190 | 0.002263 | 0.004419 |
Bud Site | 0.000528 | 0.000707 | 0.000591 | 0.002677 | 0.000198 | 0.000370 | 0.001208 | 0.002512 | 0.000314 | 0.000411 | 0.001025 | 0.002612 |
Cell Periphery | 0.000075 | 0.000043 | 0.000046 | 0.000185 | 0.000040 | 0.000045 | 0.000101 | 0.000240 | 0.000052 | 0.000044 | 0.000085 | 0.000207 |
Cytoplasm | 0.000266 | 0.000284 | 0.000262 | 0.000778 | 0.000142 | 0.000388 | 0.000425 | 0.000661 | 0.000186 | 0.000375 | 0.000376 | 0.000732 |
Cytoplasmic Foci | 0.035796 | 0.057280 | 0.017745 | 0.058791 | 0.017840 | 0.037368 | 0.045410 | 0.050648 | 0.024169 | 0.039782 | 0.037203 | 0.055594 |
Eisosomes | 0.001112 | 0.001130 | 0.001680 | 0.000950 | 0.000804 | 0.001083 | 0.000822 | 0.001087 | 0.000912 | 0.001089 | 0.001077 | 0.001004 |
Endoplasmic Reticulum | 0.001928 | 0.000690 | 0.000996 | 0.004117 | 0.003234 | 0.000596 | 0.002226 | 0.006969 | 0.002773 | 0.000608 | 0.001861 | 0.005237 |
Endosome | 0.007832 | 0.008194 | 0.007516 | 0.044497 | 0.005725 | 0.005470 | 0.022741 | 0.045775 | 0.006468 | 0.005800 | 0.018224 | 0.044999 |
Golgi | 0.073752 | 0.093058 | 0.146925* | 0.212845* | 0.052951 | 0.083652 | 0.139115* | 0.242412* | 0.060283 | 0.084792 | 0.141432* | 0.224451* |
Lipid Particles | 0.843254* | 0.793905* | 0.728623* | 0.518055* | 0.891591* | 0.837086* | 0.703027* | 0.480117* | 0.874554* | 0.831851* | 0.710620* | 0.503162* |
Mitochondria | 0.005652 | 0.006165 | 0.008274 | 0.033280 | 0.002113 | 0.007022 | 0.009707 | 0.042275 | 0.003361 | 0.006918 | 0.009282 | 0.036811 |
Mitotic Spindle | 0.000674 | 0.002320 | 0.000224 | 0.005259 | 0.000281 | 0.001381 | 0.003346 | 0.003245 | 0.000419 | 0.001495 | 0.002420 | 0.004468 |
None | 0.000137 | 0.000216 | 0.000167 | 0.000304 | 0.000290 | 0.000194 | 0.000149 | 0.000409 | 0.000236 | 0.000197 | 0.000154 | 0.000345 |
Nuclear Periphery | 0.000666 | 0.002328 | 0.000400 | 0.002065 | 0.000550 | 0.000717 | 0.000980 | 0.002616 | 0.000591 | 0.000912 | 0.000808 | 0.002281 |
Nuclear Periphery Foci | 0.003124 | 0.003640 | 0.000226 | 0.002916 | 0.001085 | 0.002991 | 0.001581 | 0.002577 | 0.001804 | 0.003070 | 0.001179 | 0.002783 |
Nucleolus | 0.000142 | 0.002358 | 0.000032 | 0.000219 | 0.000037 | 0.000516 | 0.000133 | 0.000226 | 0.000074 | 0.000740 | 0.000103 | 0.000222 |
Nucleus | 0.000053 | 0.000274 | 0.000017 | 0.000091 | 0.000025 | 0.000093 | 0.000054 | 0.000104 | 0.000035 | 0.000115 | 0.000043 | 0.000096 |
Peroxisomes | 0.003977 | 0.006475 | 0.002534 | 0.004706 | 0.001921 | 0.005477 | 0.006200 | 0.005356 | 0.002646 | 0.005598 | 0.005112 | 0.004961 |
Vacuole | 0.001728 | 0.004463 | 0.001461 | 0.011889 | 0.000769 | 0.001752 | 0.002928 | 0.012893 | 0.001107 | 0.002081 | 0.002492 | 0.012283 |
Vacuole Periphery | 0.000539 | 0.001464 | 0.001207 | 0.007538 | 0.000436 | 0.000768 | 0.001696 | 0.011356 | 0.000472 | 0.000852 | 0.001551 | 0.009037 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.53 | -9.20 | -14.68 | -14.98 | -0.58 | 2.88 | -9.34 | -11.84 | -13.74 | -4.71 | 3.28 | -13.09 | -18.56 | -21.15 | -4.88 |
Bud Neck | -1.44 | 1.59 | -4.62 | -1.79 | -7.77 | -0.25 | -5.22 | -6.55 | -6.69 | -3.04 | 0.16 | -3.57 | -9.04 | -9.86 | -5.87 |
Bud Site | -0.57 | -0.25 | -4.95 | -3.65 | -4.39 | -2.77 | -7.04 | -7.67 | -7.00 | -3.95 | -1.29 | -5.48 | -8.08 | -7.63 | -5.23 |
Cell Periphery | 1.56 | 1.46 | -5.11 | -11.40 | -11.42 | -0.66 | -6.13 | -10.93 | -10.44 | -7.05 | 0.79 | -3.37 | -12.77 | -14.65 | -10.55 |
Cytoplasm | -0.31 | 0.10 | -3.62 | -3.59 | -3.85 | -3.24 | -11.62 | -2.95 | -1.45 | -1.36 | -2.76 | -7.60 | -5.05 | -2.92 | -3.37 |
Cytoplasmic Foci | -4.08 | 7.98 | -8.34 | -0.26 | -17.75 | -12.26 | -14.62 | -14.35 | -5.38 | -1.95 | -9.85 | -8.48 | -18.20 | -8.09 | -9.63 |
Eisosomes | -0.07 | -2.51 | 0.92 | 0.92 | 4.65 | -1.11 | -0.12 | -1.77 | -0.01 | -1.98 | -0.81 | -1.31 | -0.80 | 0.43 | 0.82 |
Endoplasmic Reticulum | 2.70 | 2.00 | -3.36 | -7.04 | -6.32 | 3.82 | 1.31 | -2.93 | -5.89 | -4.19 | 4.55 | 1.71 | -3.54 | -8.95 | -5.94 |
Endosome | -0.21 | 0.27 | -18.38 | -15.55 | -18.67 | 0.29 | -11.68 | -17.38 | -17.82 | -9.03 | 0.91 | -10.83 | -25.77 | -26.46 | -15.85 |
Golgi | -2.48 | -9.68 | -22.33 | -14.11 | -7.89 | -9.03 | -18.30 | -27.01 | -22.19 | -13.14 | -7.89 | -20.18 | -36.76 | -29.67 | -15.47 |
Lipid Particles | 3.49 | 9.77 | 33.34* | 18.51 | 17.17 | 9.32 | 24.44 | 41.17* | 35.41* | 19.78 | 8.03 | 25.28 | 55.28 | 46.72* | 26.16* |
Mitochondria | -0.23 | -1.22 | -9.55 | -8.52 | -8.01 | -4.81 | -5.70 | -10.63 | -9.09 | -8.20 | -3.55 | -5.13 | -15.06 | -12.84 | -11.49 |
Mitotic Spindle | -1.91 | 2.05 | -4.42 | -2.24 | -4.88 | -3.03 | -3.93 | -3.41 | -1.98 | 0.12 | -3.19 | -3.62 | -5.66 | -3.85 | -2.36 |
None | -2.67 | -1.65 | -6.42 | -2.49 | -5.16 | 0.54 | 0.79 | -0.62 | -5.77 | -7.16 | 0.34 | 0.71 | -0.91 | -6.35 | -8.87 |
Nuclear Periphery | -1.98 | 1.56 | -4.67 | 0.30 | -6.12 | -1.21 | -2.10 | -3.57 | -3.31 | -2.74 | -2.11 | -1.44 | -5.90 | -4.57 | -4.89 |
Nuclear Periphery Foci | -0.38 | 3.70 | 0.16 | 0.52 | -5.42 | -3.01 | -1.10 | -3.13 | 0.56 | -1.64 | -2.04 | 1.43 | -2.19 | 0.42 | -3.56 |
Nucleolus | -1.71 | 3.06 | -1.20 | 1.64 | -3.03 | -3.01 | -2.55 | -2.69 | 1.68 | -1.20 | -3.11 | -1.00 | -3.11 | 2.37 | -2.31 |
Nucleus | -1.81 | 2.33 | -1.81 | 1.50 | -4.57 | -3.19 | -2.44 | -2.36 | -0.26 | -1.45 | -3.30 | -0.82 | -3.57 | 0.69 | -3.03 |
Peroxisomes | -1.20 | 1.62 | -0.93 | 0.86 | -2.87 | -4.58 | -5.19 | -3.70 | 0.14 | 0.84 | -4.22 | -3.86 | -4.03 | 0.89 | 0.21 |
Vacuole | -1.77 | 0.37 | -9.55 | -3.92 | -8.76 | -2.66 | -5.98 | -9.67 | -8.75 | -7.85 | -2.66 | -4.24 | -13.87 | -11.95 | -11.76 |
Vacuole Periphery | -3.00 | -3.46 | -10.06 | -8.01 | -8.84 | -2.10 | -7.63 | -8.86 | -8.54 | -7.79 | -2.67 | -8.33 | -13.33 | -12.47 | -11.46 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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