Standard name
Human Ortholog
Description Farnesyl-diphosphate farnesyl transferase (squalene synthase); joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0.06 0 0 0.07 0.07 0.08 0.07 0.11 0.07 0.12 0 0 0 0 0 0
Cytoplasm 0.76 0.86 0.74 0.61 0.57 0.39 0.4 0.51 0.39 0.35 0.4 0.37 0.39 0.71 0.71 0.72 0.78 0.81 0.37 0.53 0.13 0.13 0.17 0.22
Endoplasmic Reticulum 0.2 0.07 0 0.08 0.05 0.09 0.05 0 0.1 0.11 0.14 0.1 0.61 0.25 0.25 0 0 0 0.43 0.21 0.71 0.54 0.5 0.43
Endosome 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.07 0.16 0.32 0.27 0.46 0.47 0.42 0.54 0.54 0.5 0.46 0 0 0 0.06 0.06 0 0.08 0.12 0.08 0.19 0.18 0.14
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.09 0.11 0.28 0.2 0.22 0.15 0.14 0.12 0.12 0.18 0 0 0 0.08 0.08 0.07 0 0 0 0.05 0.07 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 1 1 1 1 2 1 1 1 0 1 2 5 4 1 1 6 7 1 3 4
Bud 2 3 3 2 5 13 21 24 15 26 21 16 0 1 1 2 2 1 0 1 0 1 2 2
Bud Neck 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 1 1 2 3 1 1 3
Bud Site 0 0 0 0 0 1 2 3 2 4 1 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 4 8 11 17 18 20 17 21 13 15 35 2 14 13 16 9 9 0 3 2 1 4 10
Cytoplasm 66 211 199 158 236 187 262 347 127 132 140 192 11 130 125 104 100 60 41 137 40 15 36 58
Endoplasmic Reticulum 17 16 7 21 21 42 30 14 32 41 47 52 17 46 44 5 4 0 48 53 228 66 106 112
Endosome 0 1 12 8 20 12 24 20 3 3 4 9 0 2 0 1 0 1 3 3 2 0 1 1
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 2 0 0 1 0
Mitochondria 6 16 44 82 112 219 309 281 178 203 173 238 0 0 0 9 8 1 9 32 26 23 37 37
Nucleus 0 0 0 0 1 4 6 17 5 6 4 4 0 1 1 1 4 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 1 15 9 12 7 8 4 11 0 0 0 1 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 3 0 4 0 3 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 2 1 2 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 2 4 0 1 0 0 0 1 0 1 0 0 0 2 0 0 0 0
Vac/Vac Membrane 0 5 25 29 115 97 142 100 46 47 42 91 0 6 6 12 10 5 3 11 4 5 15 22
Unique Cell Count 87 245 269 260 416 477 654 676 328 378 347 514 28 182 177 145 128 74 114 261 323 122 214 261
Labelled Cell Count 92 258 298 313 530 612 830 845 438 489 452 654 30 203 192 159 142 81 114 261 323 122 214 261


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 4.4 5.1 5.0 5.5 4.9 5.0 5.4 5.1 5.1 5.0 5.0 6.3 6.2 6.5 6.5 6.2 6.7 5.5 5.6 6.1
Std Deviation (1e-4) 0.6 1.1 1.5 1.2 1.7 1.1 1.3 1.3 1.6 1.5 1.1 1.3 1.0 0.9 0.9 1.1 1.1 1.3 0.8 0.9 1.1
Intensity Change (Log2) – – – -0.04 0.09 -0.07 -0.03 0.09 -0.02 -0.02 -0.04 -0.03 0.3 0.28 0.35 0.34 0.29 0.38 0.11 0.13 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – -0.3 -0.5 -0.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.136 2.2844 2.0741 1.8482 1.8526 2.2456 3.804 4.8278 4.1516 3.6501 4.1234 4.266 2.7501 3.1903 2.915 2.8351 2.8333 2.4599
Actin 0.0008 0.0008 0.0036 0.0004 0.0004 0.003 0.0048 0.0109 0.0035 0.0318 0.0131 0.0009 0.0129 0.0002 0.0067 0 0.0048 0.0009
Bud 0 0.0001 0.0001 0 0 0.0001 0.0002 0.0002 0.0005 0.0006 0.0005 0.0008 0.0001 0 0 0 0 0
Bud Neck 0.0003 0.0002 0.0006 0.0001 0.0002 0.0029 0.0005 0.0023 0.0003 0.0032 0.0026 0.0009 0.0005 0.0001 0.0001 0 0.0004 0.0008
Bud Periphery 0.0001 0.0004 0.0001 0 0 0.0002 0.0003 0.0003 0.0006 0.001 0.0009 0.0005 0.0002 0 0 0 0.0001 0.0001
Bud Site 0.0002 0.0008 0.0038 0 0.0001 0.0002 0.004 0.005 0.005 0.0026 0.0007 0.0003 0.0049 0.0006 0.0001 0 0.0002 0.0001
Cell Periphery 0.0009 0.0059 0.0012 0.0004 0.0003 0.0005 0.0012 0.0039 0.0012 0.0025 0.0007 0.0004 0.0006 0.0005 0.0005 0.0003 0.0012 0.0012
Cytoplasm 0.1188 0.1592 0.1504 0.0622 0.203 0.1552 0.113 0.1657 0.0983 0.1074 0.1459 0.1376 0.109 0.1239 0.0602 0.009 0.1566 0.0962
Cytoplasmic Foci 0.0068 0.0032 0.0072 0.0013 0.0041 0.0051 0.0098 0.0067 0.0107 0.0262 0.0142 0.0074 0.0136 0.0015 0.0053 0.0001 0.0082 0.0035
Eisosomes 0.0001 0.0001 0.0002 0 0 0 0.0001 0.0004 0.0001 0.0007 0.0001 0 0.0007 0 0 0 0.0025 0
Endoplasmic Reticulum 0.8074 0.7332 0.7229 0.8759 0.721 0.7584 0.7612 0.725 0.7995 0.626 0.6912 0.789 0.7445 0.8585 0.891 0.9813 0.6016 0.8314
Endosome 0.0152 0.0227 0.0258 0.0132 0.0156 0.0171 0.0228 0.0198 0.0229 0.0526 0.0625 0.0156 0.012 0.0038 0.0026 0.0009 0.0107 0.0095
Golgi 0.0018 0.0018 0.0031 0.0004 0.0006 0.0015 0.0048 0.0034 0.0038 0.0184 0.0154 0.002 0.0051 0.0004 0.0043 0.0001 0.0053 0.0012
Lipid Particles 0.009 0.0061 0.0069 0.0021 0.0028 0.0038 0.0159 0.0065 0.0101 0.0297 0.0114 0.0063 0.0103 0.0025 0.0053 0.0003 0.0087 0.0225
Mitochondria 0.0003 0.0021 0.0009 0.0006 0.0004 0.0007 0.0023 0.0014 0.0013 0.0115 0.0057 0.0013 0.0016 0 0.0005 0 0.114 0.0001
None 0.013 0.0314 0.0478 0.0046 0.0211 0.0214 0.02 0.0147 0.0193 0.0171 0.0055 0.0146 0.0494 0.0007 0.0008 0.0001 0.0658 0.0187
Nuclear Periphery 0.0168 0.0183 0.0161 0.0297 0.0147 0.0202 0.0272 0.0154 0.0107 0.0448 0.0147 0.0118 0.0169 0.0047 0.0071 0.0053 0.0118 0.0076
Nucleolus 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0002 0.0003 0.0003 0.0002 0.0002 0.0001 0.0002 0 0 0 0.0001 0
Nucleus 0.0015 0.0024 0.0021 0.0035 0.0097 0.003 0.0028 0.0037 0.0025 0.0032 0.0023 0.0034 0.0017 0.0005 0.0004 0.0004 0.0017 0.0013
Peroxisomes 0.0012 0 0.0004 0 0.0001 0.0002 0.0014 0.0026 0.0009 0.0097 0.0019 0.0004 0.0069 0 0.0128 0 0.0031 0.0001
Punctate Nuclear 0.0011 0.0005 0.0008 0.0004 0.0011 0.0016 0.002 0.0016 0.0025 0.0015 0.0044 0.0026 0.0053 0.0001 0.0002 0 0.0004 0.0004
Vacuole 0.0043 0.0095 0.0051 0.004 0.0042 0.004 0.0038 0.0093 0.0051 0.0068 0.0043 0.0037 0.0028 0.0018 0.0016 0.002 0.0022 0.0038
Vacuole Periphery 0.0006 0.0013 0.001 0.0012 0.0005 0.0008 0.002 0.001 0.0008 0.0026 0.0017 0.0005 0.0007 0.0001 0.0002 0.0001 0.0005 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 154.5593 139.6443 130.4871 149.9219 127.5227 155.2998 128.6809 139.6674 144.7577 132.6607
Translational Efficiency 1.3094 1.2351 1.4664 1.3854 1.5038 1.4396 1.4614 1.2725 1.4858 1.4741

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
755 1764 1941 859 1184 1604 2719 2228 1939 3368 4660 3087

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 690.92 820.56 962.77 1072.05 576.76 779.38 1031.17 1060.75 621.21 800.95 1002.68 1063.89
Standard Deviation 91.55 114.48 114.53 198.54 99.27 106.58 127.81 145.76 111.26 112.68 127.01 162.26
Intensity Change Log 2 — 0.248090 0.478673 0.633782 — 0.434356 0.838239 0.879042 — 0.335827 0.653431 0.750558

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000106 0.000864 0.000909 0.008814 0.000173 0.000867 0.001264 0.004403 0.000147 0.000866 0.001116 0.005631
Bud Neck 0.006813 0.005177 0.010602 0.013656 0.009839 0.007308 0.004194 0.008886 0.008661 0.006192 0.006863 0.010213
Bud Site 0.003141 0.005611 0.013147 0.079745 0.005951 0.007371 0.007756 0.020181 0.004857 0.006449 0.010002 0.036756
Cell Periphery 0.000370 0.000795 0.000670 0.009842 0.000704 0.001045 0.002009 0.002074 0.000574 0.000914 0.001451 0.004235
Cytoplasm 0.414266* 0.316817* 0.306560* 0.148057* 0.279571* 0.257756* 0.149083* 0.198442* 0.332018* 0.288689* 0.214676* 0.184421*
Cytoplasmic Foci 0.255814* 0.129197 0.032098 0.045216 0.196914* 0.121573 0.005324 0.045815 0.219848* 0.125566 0.016476 0.045649
Eisosomes 0.000254 0.000533 0.000187 0.001173 0.000466 0.000621 0.000567 0.000747 0.000384 0.000575 0.000409 0.000865
Endoplasmic Reticulum 0.016312 0.279219* 0.256187* 0.460134* 0.084591 0.311850* 0.738986* 0.464318* 0.058005 0.294759* 0.537889* 0.463154*
Endosome 0.022019 0.014553 0.009331 0.024568 0.028257 0.022509 0.005444 0.029531 0.025828 0.018342 0.007063 0.028150
Golgi 0.003928 0.014438 0.000761 0.010479 0.012734 0.019734 0.002198 0.011613 0.009305 0.016960 0.001599 0.011298
Lipid Particles 0.050190 0.069213 0.020499 0.066064 0.124589* 0.103989* 0.011008 0.060566 0.095620 0.085775 0.014961 0.062096
Mitochondria 0.004981 0.007706 0.001455 0.002613 0.014922 0.016987 0.002898 0.003399 0.011051 0.012126 0.002297 0.003180
Mitotic Spindle 0.002237 0.001699 0.001422 0.008207 0.000293 0.002834 0.000332 0.006742 0.001050 0.002240 0.000786 0.007150
None 0.004517 0.004020 0.001704 0.002550 0.040673 0.003535 0.005302 0.003785 0.026595 0.003789 0.003803 0.003441
Nuclear Periphery 0.004775 0.006447 0.044826 0.001037 0.011981 0.007806 0.002341 0.003767 0.009175 0.007094 0.020037 0.003008
Nuclear Periphery Foci 0.003890 0.008177 0.037376 0.025501 0.010694 0.007206 0.008948 0.024038 0.008045 0.007715 0.020789 0.024445
Nucleolus 0.002155 0.000786 0.000826 0.000438 0.006850 0.001462 0.000166 0.000442 0.005022 0.001108 0.000441 0.000441
Nucleus 0.126466 0.075935 0.180259* 0.022890 0.115361 0.045354 0.031173 0.027283 0.119685 0.061371 0.093271 0.026061
Peroxisomes 0.003232 0.004049 0.001052 0.002194 0.005278 0.006476 0.000661 0.002708 0.004481 0.005205 0.000824 0.002565
Vacuole 0.072549 0.051669 0.079610 0.064841 0.046752 0.049453 0.019900 0.078474 0.056797 0.050614 0.044771 0.074681
Vacuole Periphery 0.001988 0.003095 0.000518 0.001981 0.003407 0.004264 0.000444 0.002785 0.002854 0.003651 0.000475 0.002561

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.30 -2.09 -6.62 -5.84 -5.74 -2.32 -6.20 -6.96 -5.21 -4.88 -3.21 -5.16 -9.58 -7.72 -7.40
Bud Neck 1.81 -3.21 -6.04 -7.78 -2.66 2.17 5.31 0.75 -1.61 -5.21 3.23 2.00 -2.31 -5.87 -4.45
Bud Site -2.26 -5.98 -13.90 -13.42 -11.89 -0.98 -1.46 -8.37 -7.40 -7.42 -1.69 -5.01 -15.97 -15.15 -13.09
Cell Periphery -3.13 -2.30 -9.05 -8.68 -8.82 -1.33 -5.15 -5.51 -4.60 -0.13 -2.20 -5.46 -10.76 -10.18 -8.40
Cytoplasm 9.22 10.20 22.16 17.01 15.68 2.43 18.10 9.89 7.74 -8.56 6.30 18.47 21.21 17.49 5.03
Cytoplasmic Foci 16.96 33.14 27.85 15.88 -4.81 11.93 37.29 27.42 17.30 -20.54 19.53 48.97 38.91 24.79 -16.71
Eisosomes -4.55 1.38 -5.22 -3.51 -5.78 -2.36 -1.45 -3.89 -1.61 -2.17 -4.23 -0.58 -6.81 -3.93 -6.30
Endoplasmic Reticulum -33.94* -31.26 -29.45 -9.33 -11.41 -23.19* -84.89* -38.98* -12.40 29.07* -36.36 -72.14 -50.69 -17.10 9.80
Endosome 4.06 7.02 -0.58 -4.32 -6.91 3.38 16.17 -0.70 -4.23 -17.63 6.14 16.88 -1.36 -7.68 -18.31
Golgi -7.14 2.73 -3.67 3.28 -8.20 -4.77 13.35 1.25 5.74 -12.11 -7.59 11.53 -1.81 6.14 -14.94
Lipid Particles -4.80 8.63 -2.41 1.03 -9.03 3.57 24.87 12.41 10.30 -19.37 2.53 25.20 8.78 8.03 -20.48
Mitochondria -1.96 2.99 1.87 5.39 -1.85 -1.09 8.82 8.38 8.58 -1.11 -0.93 9.15 8.02 10.07 -2.59
Mitotic Spindle 0.38 0.47 -2.75 -3.73 -3.86 -3.39 -0.21 -5.84 -3.12 -5.73 -1.49 0.36 -5.65 -4.93 -6.68
None 0.51 2.63 1.63 1.73 -1.80 8.94 8.52 8.91 -0.18 1.59 8.77 8.78 8.87 0.53 0.52
Nuclear Periphery -1.79 -15.24 4.87 8.76 17.37 2.91 7.51 6.12 5.16 -3.30 2.17 -7.81 6.92 7.58 14.85
Nuclear Periphery Foci -5.45 -15.98 -9.48 -7.69 3.54 3.84 1.95 -8.12 -10.86 -9.68 0.81 -11.68 -12.45 -13.26 -2.76
Nucleolus 4.65 4.07 5.55 2.40 1.98 7.32 10.59 10.16 2.45 -6.32 8.83 11.18 11.27 3.16 -0.13
Nucleus 9.40 -8.43 18.34 15.05 31.41 14.86 18.07 19.12 8.01 1.76 16.24 6.48 26.46 18.22 26.05
Peroxisomes -2.41 8.27 3.17 5.21 -3.82 -1.69 12.04 6.00 6.28 -9.52 -1.87 14.21 6.49 7.72 -9.84
Vacuole 4.72 -2.64 0.05 -4.29 2.52 -0.91 9.35 -9.28 -8.91 -18.94 2.30 3.32 -7.00 -10.05 -11.04
Vacuole Periphery -3.23 6.87 -0.00 2.42 -3.94 -2.37 11.93 1.32 3.00 -5.49 -3.15 13.73 0.84 3.00 -6.46
Standard name
Human Ortholog
Description Farnesyl-diphosphate farnesyl transferase (squalene synthase); joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway
Localization
Cell Percentages ER (30%), cytoplasm (7%), mixed (45%)
Cell Cycle Regulation Yes
cytoplasm - MA (3.5e-05)
Subcompartmental Group N/A

Erg9

Erg9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Erg9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available