Standard name
Human Ortholog
Description Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane; human RFK functionally complements the lethality of the null mutation
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.06 0.06 0 0 0 0 0 0
Cytoplasm 0.97 0.99 0.98 0.98 0.94 0.92 0.89 – 0.82 0.81 0.77 0.77 0.99 0.99 0.99 0.99 0.95 0.98 0.91 0.96 0.9 0.9 0.92 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.17 0.05 – 0.2 0.18 0.26 0.27 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.09 0 0.13 – 0.1 0.08 0.06 0 0 0 0 0 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 1 – 0 0 0 0 0 1 0 0 0 0 2 2 3 3 6 12
Bud 0 0 2 0 0 2 2 – 4 7 6 5 0 0 0 0 0 0 2 0 4 15 15 27
Bud Neck 0 0 1 0 0 0 2 – 0 1 1 0 0 0 0 0 0 0 1 0 1 1 4 4
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 2 4 5 0 7 – 4 3 6 4 3 3 0 8 6 8 0 0 1 1 4 2
Cytoplasm 353 611 390 377 544 366 602 – 377 415 321 366 433 864 725 133 100 123 338 592 359 420 971 673
Endoplasmic Reticulum 4 3 1 0 0 0 1 – 0 0 1 1 0 1 1 5 5 0 1 0 3 3 13 9
Endosome 0 2 2 0 3 2 2 – 1 6 4 1 0 0 1 0 0 0 6 1 3 1 5 6
Golgi 2 0 0 0 0 0 1 – 0 0 0 0 2 0 2 0 2 0 5 1 2 1 1 3
Mitochondria 2 2 1 3 7 67 32 – 92 92 110 127 2 0 2 0 2 0 4 8 2 6 4 6
Nucleus 0 2 1 0 3 3 5 – 6 8 7 8 0 0 2 4 1 1 0 0 0 1 0 1
Nuclear Periphery 0 0 0 0 0 0 2 – 1 0 0 1 0 0 0 0 0 0 0 0 1 1 3 3
Nucleolus 0 0 0 1 1 0 0 – 0 1 0 2 0 0 0 0 0 0 0 0 0 0 1 2
Peroxisomes 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 1 0 0 0 1 – 1 2 3 3 0 0 0 0 0 0 0 1 1 2 12 13
Vac/Vac Membrane 11 3 7 10 55 9 88 – 46 42 27 21 7 9 16 1 6 2 4 2 0 1 3 4
Unique Cell Count 364 620 396 386 581 399 678 458 515 417 473 438 869 736 134 105 125 373 618 398 467 1059 783
Labelled Cell Count 373 624 409 395 618 449 746 532 578 486 539 447 878 749 151 122 134 373 618 398 467 1059 783


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 5.4 6.0 5.8 6.7 4.6 5.6 – 4.6 5.0 4.7 4.7 6.9 6.6 7.1 7.2 8.1 7.3 5.8 6.0 6.4
Std Deviation (1e-4) 1.0 0.7 1.1 1.4 1.8 1.2 1.3 – 1.0 1.2 1.2 0.9 1.7 1.0 1.6 1.2 1.4 1.4 1.5 1.5 2.1
Intensity Change (Log2) – – – -0.05 0.16 -0.4 -0.11 – -0.38 -0.29 -0.36 -0.35 0.19 0.13 0.23 0.26 0.43 0.28 -0.07 -0.0 0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.8 -3.6 -4.4 -5.8 – -7.8 -8.4 -9.3 -9.2 0.5 1.7 0 0.7 -2.0 -0.1
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 4.1 – 0 8.7 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0.8 4.8 0.5 6.2 – 5.0 4.2 3.4 2.2 -0.2 -1.1 0.5 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.1018 0.4098 0.3159 0.2764 0.3776 0.21 2.4213 2.5278 2.531 2.3359 2.9033 2.4641 4.9636 5.8831 5.3052 5.3708 5.0939 5.4702
Actin 0.0131 0.0003 0.0026 0.0386 0.0352 0.0003 0.0487 0.003 0.0207 0.0444 0.0185 0.0122 0.0169 0.0007 0.0008 0.003 0.0046 0.0019
Bud 0.004 0.0001 0.0054 0.0003 0.0021 0.0072 0.0011 0.0012 0.003 0.0007 0.0004 0.0006 0.0001 0.0016 0.0006 0.0002 0.0002 0.0001
Bud Neck 0.0033 0.0001 0.0004 0.0009 0.0004 0.0009 0.006 0.0005 0.0007 0.001 0.0017 0.0033 0.0102 0.0002 0.0005 0.0007 0.0003 0.0018
Bud Periphery 0.0011 0.0001 0.0008 0.0002 0.0015 0.0026 0.0016 0.0008 0.0034 0.0005 0.0005 0.0007 0.0002 0.0007 0.0007 0.0002 0.0002 0.0001
Bud Site 0.0109 0.0007 0.0046 0.0004 0.0013 0.0002 0.0087 0.0228 0.0077 0.0028 0.0012 0.0005 0.0016 0.0085 0.001 0.0004 0.0005 0.0001
Cell Periphery 0.0003 0.0001 0.0003 0.0001 0.0002 0.0004 0.0005 0.0004 0.0004 0.0002 0.0001 0.0001 0.0006 0.0005 0.0003 0.0002 0.0001 0.0001
Cytoplasm 0.62 0.7779 0.7201 0.6161 0.7272 0.7803 0.5069 0.7315 0.6314 0.5458 0.6555 0.7327 0.7445 0.798 0.7421 0.7829 0.7016 0.786
Cytoplasmic Foci 0.0224 0.0064 0.0098 0.0153 0.0222 0.0123 0.0409 0.0063 0.0199 0.0259 0.0136 0.0166 0.0128 0.0046 0.0056 0.0112 0.0149 0.0064
Eisosomes 0.0002 0 0.0001 0 0.0001 0 0.0003 0.0001 0.0002 0.0003 0.0001 0.0001 0.0006 0 0 0 0 0
Endoplasmic Reticulum 0.0302 0.0122 0.0333 0.0288 0.0095 0.0214 0.027 0.0173 0.0226 0.0462 0.0166 0.0165 0.0282 0.0109 0.0342 0.0119 0.0081 0.0148
Endosome 0.0266 0.0061 0.0141 0.0194 0.0466 0.0168 0.0259 0.004 0.0146 0.0202 0.0442 0.0067 0.008 0.0014 0.0048 0.0054 0.0049 0.0024
Golgi 0.0075 0.0002 0.0024 0.0132 0.0058 0.0005 0.0091 0.0006 0.0058 0.0034 0.0103 0.0018 0.0016 0.0002 0.0003 0.0004 0.0019 0.0001
Lipid Particles 0.0144 0.0002 0.0067 0.0034 0.01 0.0005 0.0133 0.0009 0.0026 0.0026 0.0172 0.0011 0.0038 0.0002 0.0007 0.0004 0.0059 0.0002
Mitochondria 0.0094 0.0001 0.0053 0.0026 0.006 0.0008 0.0042 0.0091 0.0013 0.0035 0.0042 0.0004 0.0003 0.0002 0.0002 0.0002 0.0004 0.0001
None 0.1854 0.1838 0.1557 0.2206 0.1113 0.1307 0.2313 0.183 0.2433 0.2398 0.1597 0.1847 0.1461 0.1623 0.1923 0.1652 0.212 0.1729
Nuclear Periphery 0.0169 0.0037 0.014 0.0137 0.0044 0.006 0.0099 0.0044 0.006 0.0139 0.0194 0.0034 0.0053 0.002 0.0054 0.0039 0.0017 0.0025
Nucleolus 0.0056 0.0001 0.0008 0.0001 0.0004 0.0002 0.0059 0.0004 0.0003 0.0003 0.0006 0.0002 0.0006 0.0003 0.0001 0.0001 0.0002 0.0001
Nucleus 0.008 0.0043 0.0081 0.0145 0.0039 0.0124 0.0158 0.0065 0.0055 0.0057 0.0081 0.0084 0.0046 0.0045 0.0063 0.0087 0.0031 0.0067
Peroxisomes 0.0123 0 0.0089 0.0065 0.0053 0.0001 0.0238 0.0014 0.0051 0.0306 0.0103 0.0014 0.0029 0.0001 0.0001 0.0003 0.0199 0.0001
Punctate Nuclear 0.0033 0.0011 0.0037 0.0026 0.0028 0.0028 0.016 0.0028 0.0033 0.0101 0.015 0.007 0.0096 0.0018 0.0019 0.0034 0.0187 0.0029
Vacuole 0.0036 0.0021 0.0022 0.0022 0.0034 0.0029 0.0025 0.0028 0.0018 0.0015 0.0016 0.0012 0.0012 0.0012 0.0019 0.001 0.0007 0.0006
Vacuole Periphery 0.0015 0.0002 0.0008 0.0006 0.0008 0.0007 0.0009 0.0002 0.0004 0.0006 0.0011 0.0002 0.0002 0.0001 0.0002 0.0002 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.833 9.008 14.4827 23.538 11.9762 20.1423 23.9085 27.1505 25.4032 23.5721
Translational Efficiency 1.1079 1.8759 1.0977 0.8034 1.2803 1.2512 0.6717 0.7183 0.8761 0.8018

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
142 1366 1739 2103 1984 2413 2616 1542 2126 3779 4355 3645

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 762.70 784.78 919.05 775.48 707.25 827.21 861.97 884.08 710.95 811.87 884.76 821.42
Standard Deviation 131.63 111.62 121.25 111.95 81.54 110.20 121.72 116.45 86.91 112.58 124.71 125.88
Intensity Change Log 2 — 0.041173 0.269028 0.023974 — 0.226033 0.285417 0.321957 — 0.133076 0.276937 0.175034

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000886 0.000258 0.000160 0.000301 0.000063 0.000045 0.000258 0.000365 0.000118 0.000122 0.000219 0.000328
Bud Neck 0.034328 0.011655 0.003978 0.006876 0.006953 0.006803 0.008337 0.006950 0.008781 0.008557 0.006596 0.006907
Bud Site 0.045867 0.010419 0.007055 0.019825 0.003121 0.002437 0.020897 0.027999 0.005976 0.005322 0.015369 0.023283
Cell Periphery 0.000608 0.000131 0.000138 0.000179 0.000108 0.000076 0.000150 0.000224 0.000141 0.000096 0.000145 0.000198
Cytoplasm 0.364221* 0.585106* 0.474273* 0.637456* 0.584569* 0.673942* 0.590225* 0.538692* 0.569852* 0.641830* 0.543924* 0.595674*
Cytoplasmic Foci 0.226975* 0.160526 0.009617 0.021837 0.146455 0.066482 0.010791 0.021721 0.151833 0.100476 0.010322 0.021788
Eisosomes 0.000341 0.000076 0.000029 0.000033 0.000097 0.000032 0.000023 0.000039 0.000113 0.000048 0.000025 0.000036
Endoplasmic Reticulum 0.013387 0.002140 0.003665 0.001234 0.000761 0.000560 0.002022 0.001833 0.001604 0.001131 0.002678 0.001487
Endosome 0.046070 0.011958 0.001828 0.001904 0.004636 0.002441 0.001309 0.003772 0.007404 0.005881 0.001516 0.002694
Golgi 0.011718 0.002012 0.000061 0.000434 0.000596 0.000279 0.000266 0.003443 0.001339 0.000905 0.000184 0.001707
Lipid Particles 0.030487 0.003693 0.001070 0.000733 0.004906 0.001120 0.000767 0.000884 0.006615 0.002050 0.000888 0.000797
Mitochondria 0.009520 0.001383 0.000474 0.000598 0.000919 0.000437 0.001233 0.001396 0.001494 0.000779 0.000930 0.000936
Mitotic Spindle 0.008892 0.001202 0.002676 0.012209 0.001524 0.000549 0.013298 0.010609 0.002016 0.000785 0.009057 0.011532
None 0.002023 0.004668 0.005815 0.007895 0.003755 0.007441 0.003661 0.008694 0.003640 0.006439 0.004521 0.008233
Nuclear Periphery 0.001388 0.000411 0.001787 0.000740 0.000408 0.000488 0.001390 0.001016 0.000474 0.000460 0.001549 0.000857
Nuclear Periphery Foci 0.003746 0.001638 0.001836 0.000964 0.000517 0.000459 0.001076 0.001100 0.000732 0.000885 0.001380 0.001021
Nucleolus 0.004728 0.000941 0.000415 0.000444 0.000557 0.000566 0.000259 0.000398 0.000835 0.000701 0.000321 0.000425
Nucleus 0.100512 0.112296 0.418276* 0.182637* 0.199804* 0.206737* 0.272913* 0.271873* 0.193172* 0.172599* 0.330958* 0.220388*
Peroxisomes 0.014006 0.003161 0.000115 0.000574 0.001383 0.000596 0.000243 0.000728 0.002226 0.001523 0.000192 0.000639
Vacuole 0.077429 0.085243 0.066403 0.102222 0.038230 0.028281 0.069680 0.097022 0.040848 0.048871 0.068372 0.100022
Vacuole Periphery 0.002866 0.001085 0.000330 0.000905 0.000637 0.000228 0.001200 0.001243 0.000786 0.000538 0.000853 0.001048

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 3.57 3.88 3.35 -0.30 -1.23 2.94 -5.68 -7.95 -8.45 -2.09 -0.35 -5.08 -5.59 -5.61 -1.82
Bud Neck 2.59 3.47 3.27 4.38 -2.21 0.19 -0.76 0.56 0.32 1.36 0.24 2.44 2.64 2.91 0.36
Bud Site 3.54 3.98 3.15 -2.16 -4.44 1.06 -7.92 -6.49 -7.06 -0.29 0.66 -4.46 -6.23 -7.92 -2.07
Cell Periphery 6.06 5.94 5.79 -1.10 -0.48 5.86 -3.03 -2.24 -3.64 -1.12 6.10 0.79 -0.69 -4.40 -1.24
Cytoplasm -9.25 -2.28 -8.11 3.10 -15.08 -12.32 7.46 15.43 26.33 9.26 -10.43 12.62 8.70 21.20 -3.72
Cytoplasmic Foci 4.05 13.96 13.20 28.05 -9.89 18.59 35.85 33.94 21.76 -2.65 11.86 38.45 35.86 33.56 -8.95
Eisosomes 6.50 7.47 7.40 8.89 -1.17 14.77 15.79 10.98 -5.65 -7.48 12.55 16.11 13.88 2.73 -6.88
Endoplasmic Reticulum 2.54 1.97 2.68 2.07 11.59 2.27 -12.50 -11.90 -13.07 0.23 1.39 -5.57 -1.29 -6.48 9.53
Endosome 3.75 4.88 4.92 8.54 2.12 5.70 10.05 5.09 0.39 -2.98 1.87 8.25 7.57 8.10 -2.19
Golgi 2.51 3.02 2.96 4.29 -1.83 3.77 4.90 -1.31 -1.80 -1.95 1.48 4.42 1.41 0.33 -2.23
Lipid Particles 3.94 4.29 4.34 10.22 3.81 7.86 8.10 8.16 1.17 0.45 6.96 8.44 8.69 9.43 3.30
Mitochondria 1.95 2.16 2.15 4.41 -0.23 2.86 -0.70 -0.15 -2.94 0.53 2.18 1.90 2.38 0.64 1.19
Mitotic Spindle 2.44 1.24 -0.54 -6.21 -3.25 1.30 -6.35 -4.43 -5.36 1.30 1.71 -5.89 -6.31 -8.31 -0.79
None -2.49 -3.03 -3.58 -1.52 -1.10 -4.11 0.78 -3.71 0.59 -4.65 -4.11 -0.95 -4.20 0.05 -4.02
Nuclear Periphery 2.76 -2.87 0.18 -13.43 10.82 -0.77 -19.49 -17.29 -9.20 3.13 -0.01 -25.47 -18.47 -12.13 9.58
Nuclear Periphery Foci 2.49 1.86 3.19 2.08 4.74 0.76 -7.67 -6.50 -6.49 1.15 -1.33 -7.66 -4.51 -2.45 4.38
Nucleolus 1.72 2.01 1.98 4.76 -0.68 -0.02 7.24 2.55 2.37 -1.59 0.75 3.48 2.85 5.15 -2.17
Nucleus -1.06 -25.47 -8.91 -17.44 28.92 -1.47 -15.52 -16.36 -15.32 -4.37 4.17 -28.76 -12.18 -17.60 16.17
Peroxisomes 4.03 5.20 5.08 8.67 -2.64 4.68 7.78 4.96 1.41 -1.24 2.74 8.78 7.28 7.51 -2.59
Vacuole -0.81 -2.51 -8.33 -14.21 -10.09 4.60 -21.15 -20.97 -22.75 -6.21 -4.29 -23.32 -31.51 -29.22 -11.63
Vacuole Periphery 3.13 4.64 3.97 2.20 -1.47 1.74 -0.25 0.13 -1.37 0.36 1.11 0.81 0.81 -0.23 -0.09
Standard name
Human Ortholog
Description Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane; human RFK functionally complements the lethality of the null mutation
Localization
Cell Percentages cytoplasm (50%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Fmn1

Fmn1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fmn1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available