Standard name
Human Ortholog
Description Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.06 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.97 0.97 0.85 0.71 0.37 0.4 – 0.34 0.28 0.27 0.25 0.98 0.96 0.95 0.93 0.91 0.95 0.59 0.79 0.49 0.71 0.69 0.72
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.09 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0.08 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.26 0.05 0.69 0.44 – 0.67 0.73 0.73 0.77 0 0 0 0 0 0 0.2 0.11 0.3 0.15 0.15 0.13
Nucleus 0 0 0 0 0 0 0.05 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.11 0.05 0.13 – 0.06 0.07 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.06 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 9 1 3 3 11 6
Bud 1 0 0 2 2 7 6 – 6 13 8 5 1 1 0 0 0 0 2 0 1 4 10 3
Bud Neck 4 0 5 1 6 2 3 – 0 3 3 3 0 0 1 1 2 0 1 0 0 2 3 9
Bud Site 0 0 0 0 0 0 1 – 2 0 2 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 2 1 3 0 0 – 3 0 0 1 0 0 5 4 1 1 4 0 2 2 4 5
Cytoplasm 308 222 155 76 66 65 92 – 65 62 42 46 285 462 387 154 123 120 212 184 80 214 362 307
Endoplasmic Reticulum 1 3 0 0 2 0 0 – 0 0 1 1 0 1 3 15 10 5 4 0 3 2 5 4
Endosome 3 1 1 0 3 1 18 – 1 1 1 1 1 11 14 3 3 1 16 4 5 5 11 6
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 3 0 1 0 1 1
Mitochondria 13 8 0 23 5 122 102 – 130 160 116 140 1 2 1 7 1 5 70 25 49 45 79 57
Nucleus 0 1 2 1 0 3 11 – 8 6 6 9 0 1 2 1 0 0 0 0 0 0 0 1
Nuclear Periphery 0 0 0 1 0 0 0 – 1 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 1 0 1 0 0 0 0 0 0 0 0 1 0 0
Peroxisomes 0 0 0 0 0 0 3 – 2 1 2 0 1 0 5 1 0 3 0 0 0 0 2 0
SpindlePole 1 0 0 0 10 9 29 – 12 15 9 10 1 1 4 1 1 1 9 0 3 7 16 7
Vac/Vac Membrane 2 0 2 0 3 4 14 – 6 11 1 1 1 6 10 5 6 2 8 5 1 2 6 10
Unique Cell Count 323 230 159 89 93 178 230 194 219 158 183 291 479 408 166 135 126 358 233 163 304 526 430
Labelled Cell Count 335 236 167 105 100 213 279 236 272 192 219 292 485 432 192 149 138 358 233 163 304 526 430


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 3.6 4.9 4.5 5.2 3.6 4.6 – 4.2 3.8 3.7 3.7 4.3 4.4 4.8 4.9 5.1 5.0 3.8 4.0 4.1
Std Deviation (1e-4) 0.7 0.6 1.6 2.2 2.0 1.5 2.1 – 2.3 1.2 1.6 1.3 1.1 1.0 1.3 1.1 1.3 1.3 1.2 1.6 1.4
Intensity Change (Log2) – – – -0.11 0.09 -0.42 -0.08 – -0.21 -0.36 -0.38 -0.41 -0.19 -0.16 -0.02 0.01 0.06 0.03 -0.36 -0.28 -0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.3357 -0.8665 -1.1092 -1.1305 -1.2962 -1.0497 -0.4241 -0.0169 -0.0807 -0.2222 -0.0833 -0.0582 1.7277 2.0762 2.2283 2.1879 1.8101 1.9882
Actin 0.0025 0.0029 0.002 0.0007 0.0007 0.0017 0.0083 0.0014 0.0021 0.0012 0.0102 0.0008 0.0048 0.0032 0.0141 0.0114 0.0004 0.0013
Bud 0.0003 0.0009 0.0004 0.0025 0.0006 0.0008 0.001 0.0026 0.0028 0.0008 0.0008 0.0023 0.0005 0.0002 0.0064 0.0009 0.0006 0.0014
Bud Neck 0.0009 0.0015 0.0013 0.0006 0.0002 0.0082 0.0023 0.0005 0.0013 0.0001 0.0005 0.0035 0.0015 0.0006 0.0014 0.0006 0.0002 0.0013
Bud Periphery 0.0008 0.0032 0.001 0.0028 0.0012 0.0017 0.0022 0.0047 0.0045 0.0017 0.0015 0.0024 0.0009 0.0005 0.0034 0.0016 0.0016 0.0028
Bud Site 0.0044 0.0058 0.0017 0.0025 0.0013 0.0033 0.0036 0.0094 0.0065 0.0002 0.0003 0.0002 0.0092 0.0037 0.0186 0.0012 0.0004 0.0004
Cell Periphery 0.001 0.0005 0.0004 0.0005 0.0004 0.0004 0.0005 0.0004 0.0005 0.0001 0.0003 0.0001 0.0016 0.0009 0.0006 0.0002 0.0006 0.0011
Cytoplasm 0.057 0.0726 0.0554 0.081 0.0595 0.1003 0.0995 0.1566 0.1075 0.1195 0.0725 0.1324 0.1058 0.1467 0.1132 0.1715 0.183 0.1133
Cytoplasmic Foci 0.0562 0.0467 0.0495 0.0643 0.0606 0.0621 0.0727 0.0371 0.0486 0.0395 0.0299 0.0546 0.0433 0.0218 0.0283 0.0934 0.0218 0.0379
Eisosomes 0.0008 0.0009 0.0004 0.0003 0.0005 0.0002 0.0005 0.0004 0.0005 0.0001 0.0004 0.0001 0.0006 0.0004 0.0005 0.0002 0.0004 0.0002
Endoplasmic Reticulum 0.0038 0.0015 0.0013 0.0008 0.0032 0.0017 0.003 0.0014 0.0019 0.0011 0.0029 0.0008 0.0034 0.0015 0.0021 0.0031 0.0033 0.0013
Endosome 0.0315 0.0164 0.0164 0.0136 0.0431 0.021 0.0306 0.0149 0.0195 0.0482 0.0139 0.0173 0.0176 0.0075 0.0051 0.0234 0.0131 0.0294
Golgi 0.0033 0.0027 0.002 0.0039 0.003 0.0023 0.0036 0.0018 0.0025 0.0042 0.0069 0.0025 0.0043 0.0013 0.0017 0.0042 0.0019 0.0011
Lipid Particles 0.0096 0.0036 0.0058 0.0019 0.0028 0.0111 0.0075 0.0011 0.0038 0.0015 0.0043 0.0018 0.0078 0.001 0.004 0.0037 0.0047 0.0037
Mitochondria 0.0225 0.0167 0.0261 0.0364 0.0323 0.0265 0.0039 0.0165 0.0116 0.0084 0.0059 0.0148 0.0025 0.0018 0.0023 0.0132 0.0041 0.0031
None 0.7912 0.8072 0.814 0.7687 0.7691 0.7411 0.7369 0.7397 0.7672 0.7536 0.8256 0.7574 0.7678 0.8046 0.772 0.6329 0.7568 0.7945
Nuclear Periphery 0.0011 0.0008 0.0007 0.001 0.0029 0.0005 0.0027 0.0006 0.0013 0.0007 0.0067 0.0003 0.001 0.0004 0.0035 0.0022 0.0005 0.0006
Nucleolus 0.0009 0.0032 0.0011 0.0013 0.0007 0.0007 0.0015 0.0007 0.0011 0.0003 0.0006 0.0002 0.0009 0.0004 0.0018 0.0005 0.0003 0.0002
Nucleus 0.0017 0.0024 0.0015 0.0028 0.002 0.0013 0.0032 0.0033 0.002 0.0017 0.0014 0.0008 0.001 0.0006 0.0028 0.002 0.0008 0.0006
Peroxisomes 0.0026 0.0053 0.0086 0.0081 0.0087 0.0091 0.0057 0.0015 0.007 0.005 0.0041 0.0048 0.0125 0.0007 0.0044 0.0116 0.001 0.0007
Punctate Nuclear 0.0029 0.0014 0.0064 0.0049 0.0043 0.0034 0.0045 0.0015 0.0031 0.0006 0.0102 0.0014 0.0024 0.0003 0.012 0.0188 0.0005 0.0007
Vacuole 0.0042 0.0033 0.0034 0.0012 0.0023 0.002 0.0052 0.0035 0.0039 0.0097 0.0007 0.0011 0.01 0.0019 0.0015 0.0025 0.0034 0.0037
Vacuole Periphery 0.0009 0.0005 0.0005 0.0003 0.0008 0.0006 0.0009 0.0005 0.0008 0.0019 0.0004 0.0003 0.0007 0.0002 0.0004 0.001 0.0007 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.0962 1.8165 2.6959 5.9333 5.2693 6.0928 6.4284 4.9586 6.4708 7.2383
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
252 1440 2037 117 834 2324 225 1393 1086 3764 2262 1510

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 623.78 699.28 799.25 874.69 651.43 706.86 835.20 850.65 645.01 703.96 802.83 852.51
Standard Deviation 65.86 74.29 95.42 91.04 68.94 80.83 97.00 104.50 69.23 78.48 96.18 103.72
Intensity Change Log 2 — 0.164833 0.357610 0.487735 — 0.117814 0.358512 0.384955 — 0.141005 0.358070 0.436146

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000199 0.000761 0.000152 0.000866 0.000076 0.000623 0.000741 0.000367 0.000105 0.000675 0.000210 0.000406
Bud Neck 0.038014 0.053037 0.007547 0.003019 0.007460 0.051940 0.002475 0.004128 0.014550 0.052360 0.007043 0.004042
Bud Site 0.004625 0.023645 0.008631 0.011072 0.001263 0.019799 0.007197 0.029053 0.002043 0.021270 0.008488 0.027660
Cell Periphery 0.000234 0.000178 0.000098 0.000173 0.000180 0.000179 0.000262 0.000197 0.000193 0.000179 0.000115 0.000195
Cytoplasm 0.342722* 0.315548* 0.488686* 0.404391* 0.479186* 0.418948* 0.342549* 0.561769* 0.447520* 0.379390* 0.474150* 0.549575*
Cytoplasmic Foci 0.357471* 0.327657* 0.023566 0.047145 0.247627* 0.235381* 0.059228 0.036892 0.273115* 0.270683* 0.027113 0.037687
Eisosomes 0.000444 0.000222 0.000023 0.000110 0.000215 0.000230 0.000125 0.000051 0.000268 0.000227 0.000034 0.000056
Endoplasmic Reticulum 0.000553 0.001092 0.001424 0.003262 0.000689 0.000474 0.004372 0.001468 0.000658 0.000710 0.001717 0.001607
Endosome 0.013704 0.030834 0.003852 0.006116 0.008043 0.013455 0.007963 0.006333 0.009357 0.020104 0.004261 0.006316
Golgi 0.002849 0.012551 0.000196 0.000170 0.002116 0.007037 0.000537 0.002773 0.002286 0.009146 0.000230 0.002571
Lipid Particles 0.012320 0.009551 0.001048 0.003701 0.015267 0.006846 0.005018 0.001635 0.014584 0.007881 0.001443 0.001795
Mitochondria 0.012720 0.029966 0.000821 0.000253 0.002513 0.019517 0.000528 0.001911 0.004882 0.023515 0.000792 0.001782
Mitotic Spindle 0.000618 0.003237 0.004403 0.019421 0.000324 0.004133 0.006029 0.014866 0.000392 0.003790 0.004565 0.015219
None 0.000988 0.001880 0.004288 0.003006 0.003627 0.003393 0.005023 0.005412 0.003014 0.002814 0.004361 0.005226
Nuclear Periphery 0.000390 0.000297 0.001696 0.001751 0.000519 0.000291 0.001364 0.000961 0.000489 0.000293 0.001663 0.001023
Nuclear Periphery Foci 0.000166 0.000346 0.000487 0.002148 0.000329 0.000171 0.003833 0.001604 0.000291 0.000238 0.000820 0.001646
Nucleolus 0.000922 0.001942 0.001230 0.000462 0.001039 0.001298 0.001226 0.000380 0.001012 0.001544 0.001230 0.000386
Nucleus 0.142071* 0.085559 0.407656* 0.297170* 0.194254* 0.151673* 0.420091* 0.197756* 0.182145* 0.126379 0.408893* 0.205459*
Peroxisomes 0.024993 0.027722 0.000781 0.007232 0.006465 0.014580 0.006626 0.000761 0.010764 0.019608 0.001362 0.001263
Vacuole 0.042601 0.070507 0.042856 0.188323 0.027914 0.048381 0.124504 0.129522 0.031322 0.056845 0.050977 0.134078
Vacuole Periphery 0.001397 0.003468 0.000558 0.000208 0.000895 0.001654 0.000309 0.002161 0.001011 0.002348 0.000533 0.002010

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.89 0.61 -1.66 0.58 -1.81 -3.01 -3.96 -3.81 1.82 2.77 -4.25 -3.42 -3.79 2.61 -1.25
Bud Neck -2.45 5.40 6.18 16.48 4.05 -17.81 4.91 4.12 19.97 -1.61 -16.16 4.33 7.10 25.81 4.39
Bud Site -7.78 -1.92 -0.66 4.85 0.50 -12.46 -2.29 -7.07 0.72 -3.06 -15.80 -5.60 -6.78 1.74 -3.64
Cell Periphery 2.18 5.43 2.84 1.29 -2.97 0.12 -3.86 1.53 1.55 4.29 1.11 5.52 2.11 1.64 -0.93
Cytoplasm 1.57 -7.95 1.76 0.93 7.32 5.99 9.19 -0.07 -6.46 -9.40 7.79 -0.67 -1.74 -10.01 -1.24
Cytoplasmic Foci 1.98 23.85 21.89 36.73 -1.57 1.52 22.02 30.40 42.49 4.06 0.40 37.70 36.88 59.66 -1.94
Eisosomes 4.62 9.12 6.87 5.43 -8.89 -0.69 3.55 10.04 13.55 8.07 2.20 13.97 12.60 16.68 -8.56
Endoplasmic Reticulum -3.12 -6.55 -8.46 -7.14 -5.96 2.11 -8.60 -8.22 -11.88 6.74 -0.57 -9.34 -10.75 -11.00 0.37
Endosome -7.42 5.75 4.80 14.43 -0.97 -5.14 0.13 4.35 10.10 3.58 -10.35 6.79 6.17 17.32 -0.21
Golgi -8.33 5.11 5.22 11.95 1.31 -5.72 3.18 0.98 6.64 -1.96 -9.56 5.02 1.58 10.55 -2.45
Lipid Particles 2.01 9.29 6.82 7.69 -5.31 4.82 5.64 8.13 12.64 6.52 4.86 9.79 9.69 16.98 -0.88
Mitochondria -3.84 3.11 3.25 12.24 5.77 -11.58 4.84 2.22 12.44 -1.67 -11.78 4.32 3.65 16.78 -0.87
Mitotic Spindle -2.83 -4.11 -1.32 -0.89 -0.68 -4.36 -1.06 -5.89 -3.76 -2.13 -5.38 -4.94 -6.01 -4.11 -3.65
None -3.11 -8.52 -2.34 -0.60 2.94 0.27 -0.22 -0.66 -1.15 -0.19 0.30 -1.53 -1.32 -1.94 -0.16
Nuclear Periphery 1.37 -12.94 -7.18 -7.59 -2.18 4.13 -8.29 -12.82 -17.94 0.71 4.40 -13.91 -15.10 -19.75 1.84
Nuclear Periphery Foci -4.09 -7.77 -6.79 -6.13 -5.39 3.37 -6.87 -6.38 -7.50 5.15 1.40 -7.99 -7.59 -8.02 -2.59
Nucleolus -2.94 -1.40 2.89 4.52 3.05 -1.54 -0.32 5.26 8.07 3.06 -3.08 -1.18 6.10 8.16 3.68
Nucleus 5.53 -23.26 -6.69 -9.55 4.13 5.74 -10.92 -4.71 -11.96 8.97 9.19 -28.47 -7.44 -18.05 20.81
Peroxisomes -0.55 5.02 3.98 8.29 -1.13 -5.89 0.73 5.53 16.17 1.85 -5.42 6.72 7.04 22.08 1.21
Vacuole -4.97 -1.92 -10.57 -9.60 -10.30 -8.80 -10.98 -27.17 -23.78 -2.21 -11.36 -10.76 -28.35 -24.39 -21.81
Vacuole Periphery -3.52 1.73 2.52 10.09 4.68 -1.96 1.65 -0.49 1.16 -2.11 -3.96 1.65 -0.15 3.13 -1.51
Standard name
Human Ortholog
Description Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
Localization
Cell Percentages cytoplasm (10%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Fmp33

Fmp33


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fmp33-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available