Standard name
Human Ortholog
Description Integral membrane protein localized to mitochondria; required for sporulation and maintaining sphingolipid content; similar to SUR7; FMP45 has a paralog, YNL194C, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0.05 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0.06 0.05 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 0.05 0.05 0.09 0 0 0 0.08 0.05 0.06 0 0.14 0.06 0 0 0
Cytoplasm 0.88 – 0.93 0.92 0.97 0.95 0.92 0.89 0.8 0.8 0.62 0.66 0.96 0.96 0.94 0.85 0.82 0.86 0.61 0.59 0.56 0.79 0.78 0.77
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.09 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.19 – 0 0 0 0.05 0.11 0.09 0.23 0.18 0.44 0.27 0 0 0 0 0 0 0.22 0.06 0.16 0.05 0.06 0.06
Nucleus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0.05 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0.05 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 – 2 3 1 3 2 10 14 16 9 25 5 5 14 3 2 1 3 1 6 2 7 5
Bud 0 – 2 2 0 3 5 9 16 12 10 14 2 2 4 0 2 0 0 2 9 2 9 8
Bud Neck 0 – 4 2 13 12 18 15 12 16 3 8 6 1 2 1 2 1 0 0 3 0 3 4
Bud Site 0 – 0 0 1 0 0 0 1 1 4 2 0 1 0 0 0 0 – – – – – –
Cell Periphery 0 – 4 4 3 3 3 7 12 16 11 22 7 11 14 13 8 10 2 5 12 6 8 15
Cytoplasm 129 – 197 197 344 328 491 457 305 246 134 162 275 467 474 140 141 136 105 22 114 117 288 326
Endoplasmic Reticulum 0 – 1 1 0 0 1 1 2 1 1 3 1 0 4 9 12 14 1 1 4 1 3 2
Endosome 0 – 0 0 0 1 1 0 0 0 0 0 0 2 1 1 3 4 2 0 2 2 2 2
Golgi 0 – 0 1 1 0 0 0 0 3 0 2 1 0 3 0 3 1 0 0 1 1 1 1
Mitochondria 28 – 2 2 2 16 59 47 89 56 95 67 0 2 2 1 4 6 37 2 33 6 20 27
Nucleus 0 – 1 1 2 1 2 1 3 0 1 2 0 1 0 1 0 1 0 0 0 0 2 1
Nuclear Periphery 1 – 0 0 0 0 1 0 4 4 6 6 0 0 1 0 0 0 1 0 0 0 0 0
Nucleolus 0 – 0 0 1 0 1 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 1 1 2 1 4 0 1 4 0 0 2 0 0 0 0 0 0
SpindlePole 0 – 4 3 7 2 9 9 10 7 15 12 0 4 7 6 9 4 2 0 4 1 5 8
Vac/Vac Membrane 1 – 3 3 1 1 2 2 0 2 3 5 0 1 1 9 8 6 8 2 6 2 6 7
Unique Cell Count 146 211 213 353 347 534 511 381 306 217 246 285 485 506 164 171 159 173 38 206 150 370 426
Labelled Cell Count 160 220 219 376 370 595 559 469 382 293 336 297 498 531 184 194 186 173 38 206 150 370 426


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.0 – 5.2 5.2 4.6 3.7 3.3 3.8 3.6 3.9 4.1 4.5 4.6 5.2 5.0 6.0 6.4 6.2 4.5 4.8 5.2
Std Deviation (1e-4) 0.4 – 4.5 4.5 2.6 2.0 1.5 1.5 2.2 2.5 3.3 3.2 0.9 2.6 2.0 2.4 2.8 3.0 1.1 1.6 1.6
Intensity Change (Log2) – – – 0.0 -0.18 -0.51 -0.65 -0.45 -0.56 -0.43 -0.35 -0.23 -0.18 -0.02 -0.07 0.2 0.28 0.24 -0.22 -0.13 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.7 2.8 1.6 2.2
Cytoplasm -0.4 2.4 0.6 -0.7 -1.6 -4.3 -4.1 -7.8 -7.1 1.6 1.7 0.2 -2.5 -3.3 -2.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 3.5 4.0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 1.8 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 2.2 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.1228 -0.4818 -0.2324 0.0627 -0.3831 -0.053 -1.0251 3.4144 0.1178 -1.1065 0.2485 0.8265 -1.3864 0.7025 1.2773 0.129 0.1467 1.0773
Actin 0.0693 0.0198 0.0246 0.0346 0.0795 0.0351 0.0571 0.0244 0.006 0.0019 0.0653 0.0415 0.0414 0.06 0.0577 0.0541 0.0573 0.0421
Bud 0.0072 0.0212 0.0156 0.0083 0.0076 0.0066 0.0075 0.0182 0.0066 0.0044 0.0006 0.0171 0.0052 0.021 0.01 0.0058 0.0193 0.0045
Bud Neck 0.0035 0.0032 0.0121 0.0149 0.0324 0.0217 0.0115 0.0036 0.0104 0.0031 0.0603 0.0196 0.0115 0.0066 0.0236 0.0141 0.0456 0.0358
Bud Periphery 0.0417 0.0238 0.0278 0.0311 0.04 0.0595 0.0451 0.0302 0.0204 0.0058 0.0026 0.0663 0.0213 0.0498 0.0427 0.033 0.0925 0.0582
Bud Site 0.0282 0.0957 0.0496 0.0332 0.0259 0.0031 0.0641 0.2056 0.0356 0.0575 0.0023 0.0154 0.0443 0.1947 0.0901 0.0403 0.012 0.0219
Cell Periphery 0.0258 0.0398 0.0577 0.0427 0.0708 0.057 0.0456 0.1125 0.1058 0.0269 0.0871 0.1518 0.0541 0.0772 0.1102 0.1327 0.094 0.2118
Cytoplasm 0.0636 0.0806 0.0958 0.1525 0.1374 0.1199 0.0362 0.0725 0.0868 0.0436 0.0807 0.0731 0.0574 0.0841 0.0854 0.128 0.0483 0.1335
Cytoplasmic Foci 0.0373 0.0118 0.0206 0.0164 0.0147 0.0257 0.0083 0.0033 0.0047 0.0119 0.0053 0.0053 0.0209 0.0078 0.0072 0.0179 0.0306 0.0042
Eisosomes 0.0248 0.0058 0.0148 0.0015 0.0051 0.0123 0.0095 0.0103 0.0163 0.0005 0.0312 0.0027 0.0158 0.0094 0.0238 0.0449 0.0237 0.0263
Endoplasmic Reticulum 0.0162 0.0016 0.0016 0.0071 0.0019 0.0021 0.0021 0.0006 0.0015 0.0007 0.0026 0.0016 0.0029 0.0051 0.0013 0.0016 0.0011 0.0018
Endosome 0.0325 0.0006 0.0094 0.0013 0.0421 0.012 0.007 0.0002 0.0023 0.0014 0.0185 0.0016 0.01 0.0004 0.0017 0.0108 0.0075 0.0005
Golgi 0.0145 0.0005 0.0076 0.001 0.019 0.0056 0.0024 0.0002 0.0004 0.0004 0.0084 0.0016 0.0054 0.001 0.0033 0.0096 0.0114 0.0004
Lipid Particles 0.0158 0.0007 0.0047 0.0019 0.0216 0.0141 0.0036 0.0006 0.0007 0.0013 0.0046 0.0004 0.0161 0.0008 0.0047 0.0493 0.0348 0.0007
Mitochondria 0.0283 0.0018 0.0142 0.0036 0.0129 0.0067 0.0112 0.0006 0.0018 0.0019 0.0068 0.0011 0.0029 0.0017 0.0133 0.0545 0.0066 0.0024
None 0.5198 0.6809 0.6238 0.6094 0.4723 0.5954 0.6714 0.5144 0.6786 0.8136 0.6032 0.5952 0.668 0.4762 0.5134 0.3714 0.441 0.4523
Nuclear Periphery 0.014 0.0008 0.0017 0.0041 0.0028 0.0025 0.0026 0.0002 0.0058 0.0012 0.012 0.0004 0.0014 0.0006 0.0003 0.0007 0.0005 0.0002
Nucleolus 0.0039 0.0007 0.001 0.0019 0.0003 0.001 0.0064 0.0004 0.0006 0.0012 0.0004 0.0005 0.0006 0.0003 0.0005 0.0009 0.0011 0.0002
Nucleus 0.0104 0.0043 0.0045 0.0055 0.0009 0.0057 0.0014 0.0005 0.0014 0.0025 0.0015 0.001 0.0013 0.0006 0.0006 0.0007 0.0006 0.0004
Peroxisomes 0.0223 0.0018 0.0084 0.0057 0.0056 0.0042 0.0021 0.0003 0.0005 0.0011 0.0017 0.0003 0.0107 0.0014 0.008 0.0231 0.0695 0.0006
Punctate Nuclear 0.0101 0.0029 0.0019 0.0174 0.0024 0.0041 0.0019 0.0003 0.0007 0.0172 0.0017 0.0009 0.004 0.0004 0.0006 0.0047 0.0012 0.0002
Vacuole 0.0083 0.0014 0.002 0.0045 0.0022 0.0046 0.0022 0.001 0.012 0.0017 0.0012 0.0024 0.0044 0.001 0.0014 0.0016 0.0013 0.0019
Vacuole Periphery 0.0026 0.0002 0.0004 0.0014 0.0026 0.0012 0.0006 0.0001 0.001 0.0002 0.0019 0.0002 0.0004 0.0001 0.0002 0.0005 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.1187 6.3637 3.1481 3.6774 5.384 13.0438 5.8982 6.1522 8.6897 5.3616
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
386 434 1512 558 1921 837 116 421 2307 1271 1628 979

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 628.20 770.72 735.95 789.19 647.62 719.11 899.33 782.43 644.37 736.73 747.59 786.28
Standard Deviation 65.77 231.59 217.47 728.93 135.55 138.39 121.44 139.64 126.79 177.55 216.20 557.89
Intensity Change Log 2 — 0.294983 0.228384 0.329149 — 0.151065 0.473703 0.272814 — 0.223723 0.358113 0.300828

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000201 0.034906 0.013892 0.014899 0.002876 0.007457 0.005042 0.044591 0.002428 0.016830 0.013262 0.027668
Bud Neck 0.016325 0.052144 0.011879 0.006405 0.021996 0.043893 0.003060 0.014978 0.021048 0.046710 0.011251 0.010092
Bud Site 0.003133 0.169140* 0.085327 0.071711 0.021839 0.037263 0.005906 0.056615 0.018709 0.082295 0.079668 0.065220
Cell Periphery 0.000298 0.047117 0.032217 0.014071 0.008185 0.008724 0.002251 0.009109 0.006865 0.021834 0.030082 0.011938
Cytoplasm 0.364731* 0.243784* 0.341971* 0.424167* 0.442453* 0.341537* 0.267938* 0.364767* 0.429449* 0.308158* 0.336696* 0.398623*
Cytoplasmic Foci 0.337746* 0.212099* 0.026208 0.045844 0.198794* 0.240353* 0.038247 0.075296 0.222043* 0.230705* 0.027066 0.058509
Eisosomes 0.000362 0.016366 0.002862 0.001922 0.001086 0.003325 0.007149 0.008910 0.000965 0.007778 0.003167 0.004927
Endoplasmic Reticulum 0.000616 0.003519 0.002876 0.002689 0.003680 0.002410 0.005574 0.004499 0.003167 0.002789 0.003068 0.003467
Endosome 0.016956 0.022203 0.003411 0.007090 0.012241 0.025591 0.006335 0.013954 0.013029 0.024434 0.003619 0.010041
Golgi 0.003297 0.007270 0.000426 0.001620 0.003311 0.008152 0.000102 0.005683 0.003309 0.007851 0.000403 0.003367
Lipid Particles 0.012742 0.009441 0.004957 0.002562 0.015801 0.009542 0.004943 0.010943 0.015289 0.009508 0.004956 0.006166
Mitochondria 0.004065 0.009618 0.001749 0.004314 0.005908 0.015374 0.000321 0.006660 0.005599 0.013409 0.001648 0.005323
Mitotic Spindle 0.000152 0.001975 0.004799 0.012746 0.000234 0.009045 0.000273 0.004813 0.000221 0.006631 0.004476 0.009335
None 0.001825 0.001896 0.003688 0.006374 0.004185 0.003465 0.003340 0.005131 0.003790 0.002929 0.003663 0.005839
Nuclear Periphery 0.000545 0.000274 0.001100 0.000911 0.000628 0.000498 0.001923 0.000767 0.000614 0.000421 0.001159 0.000849
Nuclear Periphery Foci 0.000350 0.000635 0.000526 0.001291 0.000252 0.000332 0.002839 0.001195 0.000268 0.000435 0.000691 0.001250
Nucleolus 0.001518 0.001217 0.000828 0.000473 0.001033 0.001909 0.000793 0.000696 0.001114 0.001673 0.000826 0.000569
Nucleus 0.180448* 0.075925 0.415034* 0.227584* 0.199134* 0.140334* 0.540778* 0.199005* 0.196008* 0.118341 0.423993* 0.215294*
Peroxisomes 0.015475 0.036168 0.001637 0.002567 0.004652 0.022404 0.000503 0.009451 0.006463 0.027104 0.001556 0.005527
Vacuole 0.038551 0.052416 0.043577 0.149128 0.050397 0.075987 0.102490 0.157782 0.048415 0.067938 0.047774 0.152849
Vacuole Periphery 0.000663 0.001886 0.001035 0.001632 0.001315 0.002409 0.000193 0.005157 0.001206 0.002230 0.000975 0.003148

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.62 -6.44 -3.11 3.80 1.01 -2.57 -0.08 -4.12 -3.32 -3.84 -5.82 -4.93 -4.69 -0.19 -1.58
Bud Neck -5.99 1.79 3.94 8.65 4.10 -5.77 10.88 3.86 7.73 -2.80 -7.96 6.31 7.59 12.42 1.81
Bud Site -11.88 -13.27 -6.25 7.79 3.36 -2.83 5.61 -2.31 -0.28 -4.73 -10.05 -9.48 -4.56 4.94 3.93
Cell Periphery -6.13 -8.09 -2.52 4.68 4.28 -0.10 3.56 0.88 0.85 -1.72 -4.35 -5.53 -0.09 3.90 5.02
Cytoplasm 6.87 2.00 -0.40 -7.27 -2.48 9.17 11.66 7.16 0.31 -5.08 13.16 12.12 7.10 -3.90 -2.59
Cytoplasmic Foci 7.59 26.82 25.19 14.97 -4.40 -4.56 22.97 20.57 19.69 -4.28 -1.17 41.75 33.65 26.46 -7.71
Eisosomes -4.21 -4.75 -1.11 3.92 1.58 -1.91 -0.75 -2.06 -0.96 -0.40 -4.46 -3.10 -2.02 2.53 -0.41
Endoplasmic Reticulum -2.30 -6.66 -8.58 -0.00 -0.84 1.20 -3.41 0.11 -0.98 3.28 0.46 -0.44 -0.50 -0.94 -0.11
Endosome -1.76 5.84 4.53 6.74 -1.56 -6.14 4.49 1.09 5.80 -1.82 -6.67 10.51 4.37 9.37 -3.47
Golgi -2.29 3.74 2.65 3.94 -1.32 -3.47 10.37 -0.20 2.61 -3.15 -4.20 9.93 2.06 4.96 -3.19
Lipid Particles 1.38 5.00 8.40 3.77 1.82 3.84 9.35 3.89 0.82 -1.34 4.20 8.13 9.17 3.58 -0.10
Mitochondria -2.92 2.04 0.78 3.38 -0.75 -3.72 7.06 0.76 3.47 -1.85 -4.30 5.15 1.67 4.91 -1.46
Mitotic Spindle -2.94 -4.17 -3.34 -2.65 -1.50 -3.83 -1.51 -3.16 0.62 -2.75 -4.19 -4.15 -4.42 -0.88 -1.71
None -0.09 -2.91 -1.81 -1.68 -0.64 0.50 1.72 -0.13 -0.50 -1.27 0.87 0.42 -0.55 -1.23 -0.91
Nuclear Periphery 3.06 -6.71 -6.65 -10.64 -1.10 1.44 -7.18 -4.20 -5.02 5.34 2.88 -9.01 -7.72 -10.04 0.99
Nuclear Periphery Foci -2.32 -2.55 -7.96 -4.70 -6.52 -1.65 -6.20 -4.43 -3.73 4.16 -3.25 -7.19 -9.52 -7.06 -4.43
Nucleolus 0.39 1.79 3.25 3.82 2.26 -3.03 1.78 3.01 4.29 0.91 -2.85 1.33 5.30 5.60 1.98
Nucleus 8.93 -19.87 -5.12 -14.21 13.71 7.19 -12.99 -1.74 -5.89 11.25 11.81 -26.78 -4.90 -12.95 18.03
Peroxisomes -3.87 5.29 5.20 7.32 -0.27 -8.02 11.50 -0.92 5.68 -3.30 -9.25 8.52 2.91 10.15 -2.64
Vacuole -2.81 -3.10 -17.21 -15.54 -16.28 -5.75 -6.00 -14.10 -11.49 -3.80 -5.83 -3.41 -21.88 -18.45 -19.91
Vacuole Periphery -3.16 -0.74 -0.59 1.32 -0.09 -3.28 8.77 -1.45 -0.64 -2.30 -3.88 0.95 -1.14 0.36 -1.48
Standard name
Human Ortholog
Description Integral membrane protein localized to mitochondria; required for sporulation and maintaining sphingolipid content; similar to SUR7; FMP45 has a paralog, YNL194C, that arose from the whole genome duplication
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Fmp45

Fmp45


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fmp45-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available