Standard name
Human Ortholog
Description Protein of unknown function; required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.06 0.08 0.12 0.15 0.12 – 0.06 0.09 0.07 0.07 0.05 0.13 0.18 0.15 0.12 0.13 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.13 0 0.08 0 0.55 0.17 – 0.48 0.38 0.59 0.57 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.96 0.9 0.91 0.83 0.8 0.61 0.73 – 0.73 0.71 0.68 0.67 0.93 0.82 0.76 0.71 0.73 0.68 0.9 0.85 0.78 0.78 0.67 0.55
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.07 0 0.05 0.07 0 0.06 – 0 0 0 0 0 0.06 0.07 0.15 0.16 0.23 0 0.07 0.15 0.13 0.21 0.25
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud 0 0 0 0 0 2 0 – 2 5 10 4 0 0 0 0 0 1 2 1 0 3 5 7
Bud Neck 0 0 1 1 0 0 0 – 0 0 2 0 0 0 0 0 0 0 0 0 0 1 1 1
Bud Site 0 0 0 0 0 7 2 – 6 2 15 12 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 9 3 8 17 39 38 49 – 24 38 23 21 21 74 112 41 28 29 2 1 5 5 4 8
Endoplasmic Reticulum 0 1 0 0 1 1 0 – 0 0 0 0 0 0 0 6 7 10 0 0 0 3 0 1
Endosome 1 0 1 6 5 0 7 – 1 3 0 1 0 4 6 7 5 8 1 2 0 1 4 9
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 1 3 0 1 1 2 0
Mitochondria 15 12 1 16 10 140 68 – 178 155 182 179 4 5 1 10 9 9 3 0 0 2 6 14
Nucleus 292 82 129 174 259 156 285 – 271 290 212 212 354 468 467 189 165 151 278 83 126 231 304 280
Nuclear Periphery 0 1 0 1 2 2 1 – 3 1 2 2 0 0 0 1 2 0 0 0 0 0 1 3
Nucleolus 0 0 0 1 0 2 0 – 0 3 0 1 0 2 1 0 0 1 0 0 0 1 13 23
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 3 2 0 3 – 7 4 2 2 0 1 1 0 1 0 0 0 0 2 12 20
Vac/Vac Membrane 2 6 6 10 22 7 24 – 10 13 6 2 7 32 45 40 37 52 10 7 24 37 97 130
Unique Cell Count 305 91 141 209 322 256 393 373 410 311 315 382 570 618 267 227 223 308 98 162 296 458 515
Labelled Cell Count 319 105 147 229 340 355 439 502 514 454 436 386 586 634 295 254 262 308 98 162 296 458 515


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 4.8 5.3 5.1 5.7 3.8 4.9 – 4.1 4.5 4.0 4.0 5.7 5.7 5.9 6.4 7.0 6.7 5.2 5.3 5.5
Std Deviation (1e-4) 0.7 0.8 1.1 1.4 1.4 1.2 1.6 – 1.2 1.7 1.6 0.9 1.2 1.0 1.4 1.4 1.3 1.7 1.5 1.4 1.3
Intensity Change (Log2) – – – -0.06 0.12 -0.49 -0.11 – -0.36 -0.24 -0.39 -0.41 0.11 0.1 0.16 0.27 0.41 0.35 -0.02 0.01 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.3 3.5 4.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.1723 -0.0395 -0.2645 -0.3076 -0.5288 -0.1971 0.9531 1.4172 1.1154 1.0931 0.5589 1.2513 2.6103 2.4605 2.4054 2.4804 2.1104 2.2968
Actin 0.0025 0 0.0001 0 0 0 0.0006 0.0001 0.0012 0 0 0.0003 0.0309 0.0001 0.0332 0.0002 0.0036 0.001
Bud 0 0 0 0 0 0 0.0002 0.0001 0.0001 0 0 0 0.0003 0.0001 0.0014 0 0 0.0001
Bud Neck 0.001 0.0002 0.0002 0.0002 0.0005 0.0008 0.003 0.0008 0.0002 0.0004 0.0002 0.0009 0.0044 0.0003 0.006 0.0002 0.0003 0.0011
Bud Periphery 0.0001 0 0 0 0.0001 0 0.0008 0 0.0001 0 0 0 0.0006 0.0002 0.0023 0 0 0.0001
Bud Site 0.0002 0.0001 0 0 0.0001 0 0.0024 0.0006 0.0002 0 0 0.0001 0.0016 0.0005 0.0212 0.0001 0.0004 0.0004
Cell Periphery 0 0 0 0 0 0 0.0002 0 0 0 0 0 0.0002 0 0.0004 0 0 0
Cytoplasm 0.007 0.0091 0.0039 0.0032 0.0025 0.0037 0.0045 0.0186 0.003 0.0034 0.0005 0.0028 0.0142 0.0277 0.0119 0.011 0.0076 0.0106
Cytoplasmic Foci 0.0029 0.0002 0.0002 0 0 0.0002 0.0076 0.0004 0.0027 0.0001 0 0.0002 0.0072 0.0001 0.0091 0.0001 0.0048 0.0005
Eisosomes 0 0 0 0 0 0 0 0 0 0 0 0 0.0003 0 0.0003 0 0.0001 0
Endoplasmic Reticulum 0.0007 0.0005 0.0006 0.0002 0.0001 0.0002 0.0008 0.0012 0.0002 0.0001 0 0.0003 0.0036 0.0015 0.0017 0.0005 0.0002 0.0014
Endosome 0.003 0.0004 0.0005 0.0003 0.0002 0.0004 0.0095 0.0011 0.003 0.0002 0 0.0006 0.0076 0.0006 0.0033 0.0003 0.0013 0.0009
Golgi 0.0004 0 0 0 0 0 0.0022 0 0.0009 0 0 0.0001 0.0031 0 0.0038 0 0.0021 0.0002
Lipid Particles 0.0016 0 0.0001 0 0 0.0001 0.0019 0 0.0013 0 0 0.0001 0.0074 0 0.0068 0 0.011 0.0003
Mitochondria 0.0004 0.0002 0.0003 0.0003 0.0004 0.0001 0.0009 0.0002 0.0004 0.0001 0.0002 0.0003 0.0018 0.0005 0.0021 0.0004 0.001 0.0077
None 0.0103 0.004 0.0174 0.0035 0.0006 0.0058 0.0102 0.013 0.0102 0.0058 0.0002 0.0014 0.0096 0.0078 0.0054 0.0047 0.0661 0.0107
Nuclear Periphery 0.0218 0.0218 0.0344 0.0208 0.0079 0.0124 0.0058 0.015 0.0097 0.0045 0.0026 0.004 0.0081 0.0129 0.0044 0.0173 0.0025 0.0033
Nucleolus 0.0063 0.0059 0.005 0.0041 0.0084 0.0098 0.0044 0.0038 0.003 0.006 0.0118 0.009 0.0078 0.009 0.005 0.0057 0.006 0.0077
Nucleus 0.9267 0.937 0.9249 0.9582 0.9684 0.9532 0.9307 0.9373 0.9567 0.964 0.9798 0.9716 0.8789 0.9341 0.8573 0.9521 0.8702 0.9429
Peroxisomes 0.0015 0 0 0 0 0 0.0023 0 0.0012 0 0 0.0001 0.0064 0 0.02 0 0.0155 0.0005
Punctate Nuclear 0.0127 0.0199 0.0117 0.0084 0.0105 0.0132 0.0107 0.0039 0.0056 0.0151 0.0044 0.0078 0.0025 0.0027 0.0033 0.007 0.0069 0.0101
Vacuole 0.0007 0.0004 0.0003 0.0003 0.0002 0.0001 0.0011 0.0033 0.0002 0.0002 0 0.0002 0.0027 0.0014 0.0008 0.0002 0.0001 0.0004
Vacuole Periphery 0.0003 0.0002 0.0004 0.0003 0.0001 0.0001 0.0003 0.0007 0.0001 0 0 0.0001 0.0009 0.0004 0.0002 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.3414 3.7792 4.2065 4.5578 5.4813 4.2253 7.1647 6.4476 8.0774 5.1695
Translational Efficiency 1.2963 1.5288 1.6753 2.4068 2.316 2.3074 0.9805 1.6341 1.2013 2.3524

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1726 842 293 249 1740 98 328 1546 3466 940 621 1795

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 742.04 765.53 959.87 949.97 689.32 838.72 952.20 890.05 715.57 773.16 955.82 898.36
Standard Deviation 86.80 115.40 97.18 119.99 87.71 95.09 110.46 105.19 91.16 115.64 104.47 109.35
Intensity Change Log 2 — 0.044962 0.371342 0.356385 — 0.283015 0.466091 0.368713 — 0.164512 0.417749 0.362335

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000057 0.000269 0.000617 0.000646 0.000077 0.000148 0.000725 0.000534 0.000067 0.000256 0.000674 0.000549
Bud Neck 0.001719 0.003308 0.004663 0.003509 0.001371 0.000766 0.002024 0.011269 0.001544 0.003043 0.003269 0.010193
Bud Site 0.000722 0.001282 0.000928 0.003604 0.000629 0.004412 0.000816 0.009502 0.000676 0.001608 0.000869 0.008684
Cell Periphery 0.000089 0.000216 0.000077 0.000186 0.000133 0.000073 0.000085 0.000142 0.000111 0.000201 0.000081 0.000148
Cytoplasm 0.048674 0.081997 0.007093 0.103175* 0.074039 0.011306 0.017224 0.052717 0.061408 0.074627 0.012444 0.059716
Cytoplasmic Foci 0.007140 0.013294 0.000202 0.004820 0.008416 0.005963 0.000851 0.000708 0.007781 0.012529 0.000545 0.001279
Eisosomes 0.000014 0.000030 0.000032 0.000061 0.000016 0.000029 0.000040 0.000023 0.000015 0.000030 0.000036 0.000028
Endoplasmic Reticulum 0.001732 0.004556 0.008936 0.011907 0.001113 0.001900 0.009639 0.006288 0.001421 0.004279 0.009307 0.007068
Endosome 0.000492 0.001540 0.000591 0.004814 0.000650 0.001145 0.000544 0.001597 0.000571 0.001499 0.000566 0.002043
Golgi 0.000203 0.000825 0.000053 0.001373 0.000769 0.000448 0.000053 0.000696 0.000487 0.000786 0.000053 0.000790
Lipid Particles 0.000995 0.001872 0.000458 0.002074 0.001695 0.007429 0.000701 0.000360 0.001346 0.002451 0.000587 0.000597
Mitochondria 0.000828 0.002007 0.000629 0.000799 0.002096 0.003253 0.000547 0.006244 0.001465 0.002137 0.000586 0.005489
Mitotic Spindle 0.000959 0.001126 0.000125 0.010180 0.000991 0.000648 0.000264 0.015256 0.000975 0.001076 0.000199 0.014552
None 0.003172 0.013313 0.001826 0.014555 0.007900 0.005928 0.003248 0.005783 0.005545 0.012543 0.002577 0.007000
Nuclear Periphery 0.002097 0.001372 0.001019 0.002202 0.001115 0.000582 0.000925 0.001969 0.001604 0.001290 0.000969 0.002001
Nuclear Periphery Foci 0.000327 0.000577 0.000333 0.003255 0.000313 0.001226 0.000534 0.000671 0.000320 0.000644 0.000439 0.001029
Nucleolus 0.001895 0.006356 0.001628 0.001270 0.002606 0.005236 0.001830 0.001466 0.002252 0.006239 0.001735 0.001439
Nucleus 0.926308* 0.858832* 0.963841* 0.762524* 0.892984* 0.943059* 0.949415* 0.869932* 0.909579* 0.867613* 0.956222* 0.855033*
Peroxisomes 0.000601 0.002561 0.000133 0.000231 0.001416 0.002970 0.000188 0.000207 0.001010 0.002604 0.000162 0.000210
Vacuole 0.001849 0.004332 0.006715 0.063792 0.001526 0.003060 0.010253 0.012856 0.001687 0.004200 0.008583 0.019922
Vacuole Periphery 0.000129 0.000333 0.000102 0.005020 0.000147 0.000419 0.000093 0.001781 0.000138 0.000342 0.000097 0.002231

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.45 -9.86 -11.40 -7.35 -3.39 -1.43 -10.07 -10.40 -6.23 3.30 -3.48 -13.97 -14.07 -5.60 2.22
Bud Neck -2.38 -7.45 -6.07 -3.80 -1.24 1.44 -2.95 -12.07 -11.97 -9.64 -2.65 -7.29 -12.65 -9.29 -8.73
Bud Site -1.82 -2.12 -5.40 -4.66 -4.61 -0.89 -1.63 -5.55 -0.75 -5.16 -1.82 -2.58 -6.03 -4.80 -5.53
Cell Periphery -4.32 0.04 -3.33 1.62 -3.38 1.20 0.99 0.21 -1.36 -1.85 -3.11 0.91 -1.18 2.66 -3.38
Cytoplasm -5.27 15.16 -1.88 1.72 -6.95 9.06 10.57 4.28 -6.47 -7.37 -2.34 15.89 1.68 3.25 -12.21
Cytoplasmic Foci -2.76 7.84 4.01 4.94 -2.99 0.62 7.11 7.75 1.66 1.18 -2.40 10.42 10.13 6.20 -1.26
Eisosomes -5.76 -6.42 -11.97 -8.86 -7.23 -2.09 -7.31 -9.90 0.01 3.98 -6.12 -9.66 -14.97 -3.09 1.59
Endoplasmic Reticulum -4.28 -9.74 -11.18 -8.31 -3.97 -1.66 -13.26 -19.95 -9.43 4.90 -4.97 -16.24 -21.04 -7.03 3.38
Endosome -4.07 -1.80 -4.28 -2.42 -3.84 -0.73 -0.39 -2.54 -0.16 -2.97 -3.81 -1.38 -4.86 -0.05 -4.40
Golgi -2.03 1.27 -0.99 0.86 -1.64 0.66 2.23 1.05 0.15 -1.62 -0.96 2.53 0.41 1.27 -1.93
Lipid Particles -3.60 3.45 -2.32 -0.20 -3.44 -1.09 2.47 4.71 1.32 2.75 -2.00 3.59 4.11 3.15 0.33
Mitochondria -1.72 0.71 -1.35 1.33 -3.55 -0.55 2.12 -2.05 -0.40 -4.45 -0.96 2.24 -3.01 -1.74 -4.50
Mitotic Spindle -0.21 1.27 -2.95 -2.90 -3.15 0.64 1.14 -6.79 -6.97 -7.11 -0.14 1.68 -7.36 -7.19 -7.73
None -6.20 2.13 -2.03 1.85 -2.63 0.63 5.07 2.56 0.23 -3.07 -4.69 5.31 -0.22 4.47 -4.91
Nuclear Periphery 1.28 2.08 -3.91 -4.82 -8.93 2.39 -0.39 -9.71 -10.64 -11.54 0.73 1.81 -6.52 -4.37 -13.26
Nuclear Periphery Foci -1.87 -0.49 -3.51 -3.08 -3.43 -0.84 -3.21 -2.73 0.45 0.11 -2.30 -2.61 -4.08 -1.46 -2.43
Nucleolus -4.41 0.72 1.36 5.40 0.84 -1.12 2.61 1.94 1.52 -0.10 -4.49 2.16 1.69 5.18 0.06
Nucleus 7.62 -4.83 9.57 5.19 11.06 -2.65 -5.24 5.18 4.70 9.44 5.27 -6.83 10.85 2.68 15.17
Peroxisomes -2.28 2.77 2.22 2.81 -2.48 -0.95 2.30 2.40 1.79 1.59 -2.01 3.10 3.13 3.20 0.39
Vacuole -3.39 -4.46 -9.19 -8.94 -7.97 -1.08 -5.51 -12.45 -9.38 -1.95 -3.66 -7.08 -14.79 -13.17 -6.63
Vacuole Periphery -4.18 0.51 -1.22 -1.04 -1.23 -1.12 1.28 -2.37 -1.27 -2.49 -4.10 1.34 -2.68 -2.06 -2.77
Standard name
Human Ortholog
Description Protein of unknown function; required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining
Localization
Cell Percentages nucleus (95%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Fyv6

Fyv6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fyv6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available