Standard name | |
---|---|
Human Ortholog | |
Description | Putative 1,3-beta-glucanosyltransferase; has similarity go other GAS family members; low abundance, possibly inactive member of the GAS family of GPI-containing proteins; localizes to the cell wall; mRNA induced during sporulation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 3 | 8 | 24 | 28 | 19 | 32 | 35 | 21 | 12 | 10 | 7 | 9 | 6 | 2 | 0 | 0 | 0 | 3 | 4 | 3 | 2 | 9 |
Bud | 0 | 1 | 7 | 2 | 5 | 20 | 10 | 26 | 13 | 24 | 11 | 10 | 3 | 4 | 3 | 1 | 0 | 0 | 0 | 3 | 3 | 2 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 4 | 6 | 10 | 26 | 22 | 20 | 15 | 29 | 24 | 9 | 14 | 13 | 31 | 41 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 |
Cytoplasm | 12 | 88 | 127 | 110 | 133 | 121 | 161 | 179 | 173 | 222 | 167 | 192 | 71 | 222 | 244 | 89 | 53 | 34 | 0 | 14 | 17 | 9 | 34 | 19 |
Endoplasmic Reticulum | 6 | 60 | 53 | 88 | 129 | 156 | 198 | 142 | 132 | 100 | 33 | 36 | 104 | 155 | 120 | 4 | 4 | 2 | 39 | 110 | 151 | 118 | 236 | 210 |
Endosome | 0 | 1 | 1 | 0 | 9 | 6 | 5 | 12 | 0 | 7 | 2 | 1 | 2 | 7 | 4 | 6 | 2 | 0 | 0 | 0 | 2 | 0 | 2 | 5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 5 | 1 | 0 | 3 | 0 | 0 | 2 | 3 |
Mitochondria | 1 | 3 | 3 | 2 | 2 | 14 | 44 | 34 | 32 | 47 | 25 | 27 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 3 | 0 | 1 | 2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 0 | 4 | 1 | 0 | 1 | 56 | 22 | 21 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 1 | 0 | 2 | 5 | 4 | 4 | 3 | 3 | 3 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 7 | 8 | 2 | 25 | 21 | 39 | 41 | 19 | 41 | 19 | 36 | 2 | 2 | 9 | 152 | 73 | 53 | 18 | 36 | 39 | 8 | 31 | 90 |
Unique Cell Count | 19 | 152 | 192 | 200 | 298 | 328 | 420 | 421 | 368 | 419 | 243 | 282 | 184 | 383 | 384 | 244 | 121 | 97 | 62 | 177 | 229 | 146 | 321 | 353 |
Labelled Cell Count | 20 | 166 | 208 | 223 | 353 | 391 | 502 | 487 | 437 | 498 | 281 | 333 | 204 | 430 | 431 | 317 | 161 | 111 | 62 | 177 | 229 | 146 | 321 | 353 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.2 | 8.7 | 7.2 | 7.7 | 9.4 | 8.5 | 7.7 | 8.0 | 7.9 | 7.3 | 6.9 | 6.9 | 9.5 | 8.8 | 8.0 | 12.7 | 14.2 | 16.2 | 8.5 | 8.2 | 8.3 |
Std Deviation (1e-4) | 1.3 | 1.9 | 1.6 | 1.5 | 1.8 | 1.6 | 1.8 | 1.6 | 1.6 | 1.6 | 1.5 | 1.4 | 2.2 | 1.9 | 1.8 | 2.8 | 3.2 | 3.3 | 1.9 | 1.4 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.09 | 0.39 | 0.24 | 0.09 | 0.15 | 0.13 | 0.01 | -0.07 | -0.07 | 0.41 | 0.29 | 0.15 | 0.82 | 0.98 | 1.17 | 0.23 | 0.18 | 0.2 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.5 | 3.1 | 3.2 | 1.8 | 3.0 | 3.5 | 2.0 | 1.9 | 1.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 1.2 | -0.9 | 1.3 | -0.1 | 1.1 | 0.5 | -0.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.9 | 2.4 | 1.7 | 0.9 | 0.3 | 2.2 | 1.4 | 0.3 | 1.0 | 1.7 | 2.3 | 3.1 | 0 | 0 | 0 |
Cytoplasm | -2.3 | -4.7 | -6.4 | -6.4 | -5.4 | -4.3 | -3.1 | 0.6 | 0.4 | -5.4 | -1.9 | -0.6 | -6.2 | -3.9 | -5.0 |
Endoplasmic Reticulum | 3.4 | 3.5 | 4.5 | 4.6 | 1.5 | 2.0 | -1.0 | -3.6 | -4.1 | 5.7 | 3.0 | 0.9 | -8.0 | -5.4 | -5.2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 1.7 | 3.8 | 3.1 | 3.3 | 4.0 | 3.7 | 3.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7.1 | 6.1 | 6.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 1.8 | 1.1 | 2.2 | 2.4 | 0.5 | 2.4 | 1.6 | 3.2 | 0 | 0 | -1.2 | 12.5 | 11.0 | 9.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.5147 | 6.1808 | 8.1301 | 7.4049 | 9.5768 | 7.8992 | 5.7912 | 6.1999 | 7.6828 | 7.9264 | 7.7363 | 7.5844 | 5.4098 | 7.8926 | 9.3677 | 11.4769 | 9.4117 | 8.7159 |
Actin | 0.0389 | 0.0042 | 0.0066 | 0.0019 | 0.0002 | 0.0002 | 0.0582 | 0.0188 | 0.0087 | 0.052 | 0.0362 | 0.0057 | 0.0514 | 0.0136 | 0.0218 | 0.0087 | 0.0002 | 0.0018 |
Bud | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0 | 0 | 0.0005 | 0.0006 | 0.0005 | 0.0003 | 0.0009 | 0 | 0.0003 | 0.0004 | 0.0001 | 0.0002 | 0.0038 | 0.0002 |
Bud Neck | 0.0009 | 0.0006 | 0.0016 | 0.0013 | 0.0005 | 0.0008 | 0.0021 | 0.0006 | 0.0002 | 0.0003 | 0.0007 | 0.0028 | 0.0002 | 0.0003 | 0.0003 | 0.0164 | 0.0095 | 0.0022 |
Bud Periphery | 0.0005 | 0.0003 | 0.001 | 0.0003 | 0.0001 | 0 | 0.0006 | 0.0008 | 0.0007 | 0.0004 | 0.0007 | 0.0001 | 0.0005 | 0.0006 | 0.0002 | 0.0008 | 0.0052 | 0.0003 |
Bud Site | 0.0021 | 0.0032 | 0.0013 | 0.0002 | 0 | 0 | 0.0043 | 0.0245 | 0.0018 | 0.0005 | 0.0035 | 0.0002 | 0.0004 | 0.0024 | 0.0004 | 0.0004 | 0.0207 | 0.0002 |
Cell Periphery | 0.003 | 0.0032 | 0.0088 | 0.0011 | 0.0014 | 0.0006 | 0.0007 | 0.008 | 0.0023 | 0.0004 | 0.0014 | 0.0014 | 0.0018 | 0.0026 | 0.002 | 0.008 | 0.0009 | 0.0014 |
Cytoplasm | 0.0459 | 0.243 | 0.1034 | 0.1119 | 0.0049 | 0.0365 | 0.0473 | 0.1822 | 0.0534 | 0.0552 | 0.0414 | 0.1714 | 0.0976 | 0.2578 | 0.1684 | 0.1624 | 0.0555 | 0.094 |
Cytoplasmic Foci | 0.0152 | 0.0122 | 0.0045 | 0.0014 | 0.0001 | 0.0003 | 0.034 | 0.0284 | 0.0118 | 0.0076 | 0.0105 | 0.0035 | 0.0078 | 0.0149 | 0.0013 | 0.0039 | 0.0119 | 0.0071 |
Eisosomes | 0.0005 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0 | 0 | 0.0007 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.686 | 0.5518 | 0.8082 | 0.8507 | 0.9768 | 0.9395 | 0.6964 | 0.6001 | 0.8568 | 0.7539 | 0.8396 | 0.7865 | 0.6902 | 0.5748 | 0.7771 | 0.7722 | 0.8223 | 0.7931 |
Endosome | 0.0223 | 0.0393 | 0.01 | 0.0051 | 0.0013 | 0.0031 | 0.0308 | 0.0166 | 0.0128 | 0.0548 | 0.0206 | 0.0041 | 0.0262 | 0.035 | 0.0037 | 0.0044 | 0.025 | 0.0178 |
Golgi | 0.012 | 0.0032 | 0.007 | 0.0007 | 0.0003 | 0.0002 | 0.0165 | 0.0101 | 0.0077 | 0.013 | 0.0132 | 0.001 | 0.0181 | 0.0024 | 0.0011 | 0.002 | 0.0032 | 0.0075 |
Lipid Particles | 0.0527 | 0.0121 | 0.0131 | 0.002 | 0.0006 | 0.0014 | 0.0193 | 0.0234 | 0.008 | 0.0101 | 0.0128 | 0.0043 | 0.0074 | 0.0159 | 0.0039 | 0.0083 | 0.0017 | 0.0046 |
Mitochondria | 0.0322 | 0.0011 | 0.0018 | 0.0002 | 0.0001 | 0.0001 | 0.0046 | 0.0044 | 0.0008 | 0.0036 | 0.0015 | 0.0001 | 0.0049 | 0.0154 | 0.0002 | 0.0001 | 0.0008 | 0.0041 |
None | 0.0049 | 0.0155 | 0.0022 | 0.0003 | 0 | 0.0002 | 0.0086 | 0.0547 | 0.0032 | 0.0114 | 0.0002 | 0.0041 | 0.053 | 0.006 | 0.0007 | 0.0002 | 0.0004 | 0.0003 |
Nuclear Periphery | 0.0276 | 0.0439 | 0.0121 | 0.0133 | 0.0068 | 0.01 | 0.0345 | 0.0051 | 0.005 | 0.0216 | 0.0063 | 0.0038 | 0.0208 | 0.011 | 0.0116 | 0.0068 | 0.0069 | 0.0119 |
Nucleolus | 0.0006 | 0.0002 | 0.0004 | 0 | 0 | 0 | 0.0009 | 0.0002 | 0.0001 | 0.0004 | 0 | 0 | 0.0004 | 0.0003 | 0 | 0 | 0.001 | 0.0001 |
Nucleus | 0.0024 | 0.011 | 0.002 | 0.0014 | 0.0004 | 0.0012 | 0.0089 | 0.0011 | 0.0007 | 0.0036 | 0.0005 | 0.0007 | 0.0032 | 0.0023 | 0.0008 | 0.0009 | 0.0031 | 0.0027 |
Peroxisomes | 0.0272 | 0.0004 | 0.0015 | 0 | 0 | 0 | 0.0125 | 0.0059 | 0.015 | 0.0017 | 0.0031 | 0.0002 | 0.0004 | 0.0008 | 0 | 0.0001 | 0.0005 | 0.0027 |
Punctate Nuclear | 0.0086 | 0.0012 | 0.0056 | 0.0002 | 0 | 0.0001 | 0.0075 | 0.0011 | 0.0008 | 0.002 | 0.0018 | 0.0002 | 0.0052 | 0.0128 | 0.0001 | 0.0004 | 0.0007 | 0.0002 |
Vacuole | 0.014 | 0.05 | 0.0076 | 0.0071 | 0.0061 | 0.0053 | 0.0094 | 0.0127 | 0.0092 | 0.0055 | 0.0047 | 0.0095 | 0.0076 | 0.0297 | 0.0058 | 0.0035 | 0.0252 | 0.046 |
Vacuole Periphery | 0.0021 | 0.003 | 0.0007 | 0.0007 | 0.0004 | 0.0004 | 0.0022 | 0.0004 | 0.0005 | 0.0018 | 0.0004 | 0.0004 | 0.0017 | 0.0009 | 0.0004 | 0.0002 | 0.0015 | 0.0018 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 62.3511 | 126.5067 | 52.5133 | 42.2015 | 69.941 | 47.6127 | 125.2196 | 100.6484 | 54.2108 | 65.5158 |
Translational Efficiency | 0.9131 | 1.0484 | 1.1975 | 0.9718 | 0.9101 | 1.0587 | 1.0525 | 0.9223 | 1.0223 | 0.99 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1866 | 910 | 122 | 1725 | 2074 | 1188 | 293 | 617 | 3940 | 2098 | 415 | 2342 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 771.50 | 948.67 | 1354.07 | 1029.69 | 925.91 | 923.63 | 1222.52 | 1003.05 | 852.78 | 934.49 | 1261.19 | 1022.67 |
Standard Deviation | 116.82 | 177.45 | 208.96 | 151.79 | 126.26 | 163.19 | 152.27 | 162.52 | 144.22 | 169.98 | 181.10 | 155.13 |
Intensity Change Log 2 | — | 0.298240 | 0.811564 | 0.416472 | — | -0.003557 | 0.400914 | 0.115450 | — | 0.141477 | 0.602128 | 0.260091 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000480 | 0.001992 | 0.001454 | 0.005741 | 0.000302 | 0.000866 | 0.001994 | 0.003681 | 0.000386 | 0.001354 | 0.001835 | 0.005198 |
Bud Neck | 0.009622 | 0.003500 | 0.003949 | 0.012814 | 0.003574 | 0.004250 | 0.002944 | 0.008152 | 0.006438 | 0.003925 | 0.003240 | 0.011585 |
Bud Site | 0.010909 | 0.016835 | 0.010225 | 0.037572 | 0.005035 | 0.012251 | 0.011767 | 0.036856 | 0.007817 | 0.014239 | 0.011313 | 0.037384 |
Cell Periphery | 0.002768 | 0.003200 | 0.003702 | 0.004295 | 0.001759 | 0.002400 | 0.003585 | 0.001733 | 0.002237 | 0.002747 | 0.003619 | 0.003620 |
Cytoplasm | 0.305269* | 0.152673* | 0.054409 | 0.278494* | 0.181645* | 0.144844* | 0.100612* | 0.263102* | 0.240194* | 0.148240* | 0.087029 | 0.274439* |
Cytoplasmic Foci | 0.067084 | 0.045571 | 0.009940 | 0.031833 | 0.031467 | 0.052390 | 0.009486 | 0.050273 | 0.048335 | 0.049432 | 0.009619 | 0.036691 |
Eisosomes | 0.000988 | 0.000333 | 0.000102 | 0.000800 | 0.000452 | 0.000481 | 0.000525 | 0.000464 | 0.000706 | 0.000417 | 0.000401 | 0.000712 |
Endoplasmic Reticulum | 0.387956* | 0.636061* | 0.617319* | 0.264482* | 0.583938* | 0.643123* | 0.712344* | 0.209606* | 0.491120* | 0.640060* | 0.684409* | 0.250025* |
Endosome | 0.013179 | 0.021366 | 0.022319 | 0.010146 | 0.016930 | 0.018878 | 0.008373 | 0.027143 | 0.015154 | 0.019957 | 0.012473 | 0.014624 |
Golgi | 0.012233 | 0.010125 | 0.001644 | 0.002591 | 0.006152 | 0.014778 | 0.000912 | 0.006019 | 0.009032 | 0.012760 | 0.001127 | 0.003494 |
Lipid Particles | 0.040259 | 0.016270 | 0.025438 | 0.024498 | 0.019759 | 0.025632 | 0.015449 | 0.017845 | 0.029468 | 0.021571 | 0.018385 | 0.022745 |
Mitochondria | 0.009540 | 0.003782 | 0.000056 | 0.001945 | 0.002615 | 0.005891 | 0.000163 | 0.001075 | 0.005895 | 0.004976 | 0.000131 | 0.001716 |
Mitotic Spindle | 0.000268 | 0.000939 | 0.002609 | 0.009289 | 0.000778 | 0.000212 | 0.000389 | 0.009300 | 0.000537 | 0.000527 | 0.001041 | 0.009292 |
None | 0.019572 | 0.005729 | 0.000036 | 0.000900 | 0.002501 | 0.002876 | 0.000144 | 0.002054 | 0.010586 | 0.004113 | 0.000112 | 0.001204 |
Nuclear Periphery | 0.003074 | 0.001338 | 0.005012 | 0.002073 | 0.003165 | 0.003214 | 0.002167 | 0.005236 | 0.003122 | 0.002400 | 0.003003 | 0.002906 |
Nuclear Periphery Foci | 0.006438 | 0.008779 | 0.086364 | 0.019891 | 0.003350 | 0.005871 | 0.038719 | 0.020447 | 0.004812 | 0.007132 | 0.052726 | 0.020038 |
Nucleolus | 0.001533 | 0.000859 | 0.000041 | 0.000288 | 0.000288 | 0.000601 | 0.000020 | 0.000330 | 0.000878 | 0.000713 | 0.000026 | 0.000299 |
Nucleus | 0.059770 | 0.009701 | 0.007415 | 0.063523 | 0.024470 | 0.008982 | 0.016464 | 0.033018 | 0.041188 | 0.009294 | 0.013804 | 0.055486 |
Peroxisomes | 0.003407 | 0.001814 | 0.000171 | 0.001771 | 0.001324 | 0.002350 | 0.000257 | 0.002193 | 0.002311 | 0.002117 | 0.000232 | 0.001882 |
Vacuole | 0.042969 | 0.054131 | 0.147360 | 0.226091* | 0.107450 | 0.045806 | 0.073506 | 0.298676* | 0.076911 | 0.049417 | 0.095217 | 0.245214* |
Vacuole Periphery | 0.002682 | 0.005002 | 0.000436 | 0.000963 | 0.003045 | 0.004307 | 0.000181 | 0.002795 | 0.002873 | 0.004608 | 0.000256 | 0.001446 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.53 | -1.23 | -6.70 | -3.79 | -3.05 | -4.81 | -3.64 | -2.77 | -2.12 | -0.69 | -6.06 | -3.61 | -7.32 | -5.21 | -3.37 |
Bud Neck | 4.93 | 2.01 | -1.83 | -7.46 | -3.31 | -0.80 | 0.36 | -3.61 | -2.57 | -3.45 | 3.36 | 2.76 | -4.94 | -7.95 | -6.83 |
Bud Site | -2.74 | -0.23 | -8.59 | -5.58 | -4.14 | -4.15 | -2.17 | -6.70 | -4.56 | -3.66 | -4.68 | -1.62 | -11.80 | -7.93 | -6.02 |
Cell Periphery | -0.74 | -0.62 | -0.89 | 0.07 | 0.32 | -1.20 | -1.65 | 1.28 | 1.86 | 2.05 | -1.29 | -1.52 | -1.28 | 0.21 | 0.97 |
Cytoplasm | 16.70 | 20.43 | 2.47 | -14.09 | -18.48 | 5.03 | 6.11 | -7.07 | -9.78 | -10.17 | 15.76 | 16.24 | -6.11 | -18.97 | -19.07 |
Cytoplasmic Foci | 5.17 | 18.09 | 11.34 | 4.14 | -9.81 | -5.82 | 9.80 | -4.70 | 1.21 | -11.03 | -0.39 | 19.50 | 6.21 | 5.24 | -13.88 |
Eisosomes | 5.19 | 7.62 | 2.83 | -2.93 | -6.24 | -0.31 | -0.21 | 0.66 | 0.89 | 0.54 | 3.70 | 1.60 | 1.77 | -1.59 | -0.77 |
Endoplasmic Reticulum | -16.30 | -5.32 | 17.72 | 30.60* | 11.11 | -4.40 | -3.65 | 30.81 | 31.38* | 21.33 | -14.62 | -7.75 | 36.00 | 44.97* | 23.98 |
Endosome | -4.41 | -4.07 | 5.46 | 7.15 | 6.15 | -1.39 | 5.80 | -1.42 | -0.59 | -4.59 | -4.27 | 1.99 | 3.88 | 6.79 | 0.74 |
Golgi | 1.64 | 13.46 | 13.39 | 7.14 | -0.51 | -6.68 | 10.23 | 1.71 | 6.59 | -2.89 | -3.98 | 17.72 | 11.53 | 11.31 | -3.27 |
Lipid Particles | 10.26 | 2.42 | 8.79 | -2.15 | 1.36 | -2.78 | 1.56 | 3.70 | 5.22 | 0.62 | 4.92 | 4.13 | 7.53 | 1.87 | 0.16 |
Mitochondria | 7.29 | 17.63 | 10.88 | 2.96 | -2.82 | -3.26 | 10.55 | 4.88 | 4.99 | -2.01 | 1.36 | 19.97 | 9.60 | 5.10 | -3.04 |
Mitotic Spindle | -1.77 | -1.21 | -5.51 | -4.86 | 0.15 | 1.10 | 0.43 | -3.08 | -3.39 | -3.23 | -0.08 | -1.16 | -6.12 | -6.17 | -1.96 |
None | 6.29 | 11.76 | 11.21 | 3.35 | -4.92 | -0.43 | 4.91 | 0.81 | 1.04 | -1.82 | 5.76 | 12.50 | 10.86 | 3.81 | -3.73 |
Nuclear Periphery | 3.45 | -2.32 | 2.28 | -2.29 | 4.09 | -0.04 | 1.60 | -0.83 | -0.61 | -1.57 | 1.10 | 0.06 | 1.29 | -0.27 | 1.09 |
Nuclear Periphery Foci | -1.90 | -8.30 | -8.48 | -5.31 | 7.20 | -3.06 | -7.68 | -8.19 | -6.00 | 4.62 | -3.21 | -10.80 | -12.82 | -8.84 | 8.01 |
Nucleolus | 1.71 | 5.41 | 4.50 | 1.92 | -6.00 | -0.96 | 12.18 | -0.02 | 0.93 | -3.56 | 0.70 | 6.46 | 4.37 | 1.88 | -7.85 |
Nucleus | 20.22 | 15.03 | -4.21 | -21.77 | -17.35 | 10.33 | 1.07 | -4.52 | -9.90 | -4.29 | 22.32 | 9.36 | -9.66 | -24.83 | -15.41 |
Peroxisomes | 4.93 | 13.12 | 5.20 | 0.85 | -4.87 | -2.88 | 7.57 | -1.13 | 1.21 | -4.16 | 0.81 | 14.67 | 2.84 | 1.76 | -5.98 |
Vacuole | -2.54 | -6.25 | -37.53 | -31.72 | -5.96 | 12.27 | 0.79 | -21.06 | -26.06 | -17.69 | 7.92 | -4.47 | -38.81 | -42.38 | -18.14 |
Vacuole Periphery | -3.79 | 8.42 | 7.01 | 7.40 | -1.71 | -2.88 | 11.32 | 2.00 | 3.86 | -3.51 | -4.79 | 14.53 | 7.60 | 9.94 | -4.63 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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