Standard name
Human Ortholog
Description Protein of unknown function; null mutant exhibits a respiratory growth defect and synthetic interactions with prohibitin (phb1) and gem1; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.13 0.06 0 0 0 0 0 0
Cytoplasm 0.92 0.96 0.97 0.97 0.95 0.89 0.9 0.89 0.79 0.84 0.74 0.73 0.99 0.98 0.98 0.18 0.16 0.13 0.59 0.74 0.58 0.66 0.7 0.68
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.17 0.15 0.1 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0.2 0 0 0 0 0 0
Mitochondria 0.06 0 0 0 0 0.14 0.09 0.09 0.31 0.2 0.36 0.34 0 0 0 0.09 0.08 0.15 0.23 0.13 0.24 0.18 0.15 0.14
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.26 0.1 0.05 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.11 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.2 0.2 0.3 0.05 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 5 2 2 13 0 2 3
Bud 1 0 1 0 2 4 4 2 7 12 8 9 0 0 0 8 3 2 0 1 9 1 6 3
Bud Neck 0 11 20 12 11 14 20 27 8 15 7 18 0 0 0 0 0 0 1 1 4 0 1 3
Bud Site 0 1 0 0 0 0 1 0 2 2 0 2 0 0 0 2 1 1 – – – – – –
Cell Periphery 0 1 6 4 0 2 3 2 4 1 0 5 0 4 0 17 21 9 0 2 7 0 0 1
Cytoplasm 174 451 474 362 386 511 625 645 500 570 251 370 180 194 259 40 26 19 138 314 285 114 138 169
Endoplasmic Reticulum 0 2 0 0 0 0 1 2 1 0 0 2 0 2 1 11 6 6 1 0 4 0 0 0
Endosome 1 0 0 1 1 4 5 2 0 0 1 1 1 0 1 38 25 15 7 5 10 2 2 3
Golgi 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 6 27 30 0 1 1 0 0 0
Mitochondria 11 16 1 4 11 78 59 67 198 136 121 174 1 0 1 19 14 23 53 56 117 30 28 34
Nucleus 0 0 0 0 1 2 2 7 4 4 7 1 1 0 2 7 9 7 0 0 0 3 1 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 1 1 0 0 0 1 0 2
Nucleolus 0 0 0 1 0 0 1 1 1 1 0 0 0 0 0 2 2 1 0 0 0 0 0 0
Peroxisomes 0 0 0 1 0 1 1 0 0 1 0 0 0 1 0 57 16 7 0 0 2 0 0 0
SpindlePole 2 5 8 3 6 12 30 25 11 29 2 18 0 0 0 15 14 17 7 3 11 3 4 8
Vac/Vac Membrane 2 2 1 2 3 10 9 6 3 2 4 3 0 0 0 45 33 45 11 9 1 5 3 11
Unique Cell Count 189 470 487 374 405 574 692 721 632 679 337 508 182 197 264 222 167 149 233 424 488 174 196 249
Labelled Cell Count 193 489 512 393 421 638 761 786 739 773 401 603 183 201 264 271 198 188 233 424 488 174 196 249


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 2.9 4.6 4.2 4.2 3.8 3.9 3.9 2.9 3.4 3.2 3.2 4.5 4.4 4.5 5.6 6.7 7.0 4.8 5.3 5.5
Std Deviation (1e-4) 0.8 0.4 1.4 1.5 1.5 1.3 1.4 1.3 0.6 1.0 1.3 0.7 1.0 0.8 1.8 1.7 2.1 1.8 1.8 2.1 2.0
Intensity Change (Log2) – – – -0.12 -0.14 -0.3 -0.22 -0.23 -0.68 -0.42 -0.52 -0.54 -0.02 -0.08 -0.03 0.28 0.55 0.6 0.07 0.2 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 10.1 9.8 12.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3358 1.3867 1.3486 1.2314 1.9971 1.5317 8.0755 3.3942 4.0225 4.2226 4.1704 3.7589 1.0503 1.5866 1.3361 1.2408 1.4807 1.408
Actin 0.0282 0.0012 0.0096 0.007 0.0363 0.0044 0.1173 0.0032 0.0474 0.007 0.0021 0.0101 0.0066 0.0004 0.007 0.0034 0.0411 0.0007
Bud 0.0009 0.0011 0.002 0.0006 0.001 0.0003 0.0029 0.0116 0.0026 0.0015 0.0015 0.0014 0.0005 0.0012 0.0017 0.0027 0.0008 0.0016
Bud Neck 0.0013 0.0096 0.0032 0.0006 0.0071 0.0026 0.0035 0.0001 0.0035 0.0004 0.0005 0.0013 0.0076 0.0002 0.0024 0.0011 0.0011 0.0044
Bud Periphery 0.0017 0.0038 0.0041 0.0012 0.0025 0.0014 0.0043 0.0202 0.0107 0.0017 0.0044 0.0032 0.0021 0.0041 0.0033 0.0107 0.003 0.0018
Bud Site 0.0034 0.0164 0.006 0.0013 0.0346 0.0004 0.1286 0.0154 0.0146 0.0097 0.0005 0.0024 0.0103 0.0055 0.0059 0.0128 0.0015 0.0003
Cell Periphery 0.0017 0.0021 0.0012 0.0018 0.01 0.0005 0.0019 0.0009 0.0028 0.0002 0.0003 0.0014 0.0009 0.0004 0.0007 0.0013 0.0002 0.0002
Cytoplasm 0.104 0.1162 0.1093 0.0909 0.1308 0.16 0.0465 0.1466 0.1371 0.0653 0.3642 0.2314 0.0424 0.0719 0.0803 0.084 0.0892 0.0958
Cytoplasmic Foci 0.0338 0.0208 0.0459 0.0766 0.0611 0.0424 0.1168 0.0095 0.033 0.0283 0.0344 0.0309 0.0134 0.0353 0.0228 0.0507 0.0274 0.0484
Eisosomes 0.0016 0.0011 0.0016 0.0008 0.0019 0.0003 0.0016 0.0005 0.0011 0.0002 0.0002 0.0002 0.0005 0.0002 0.0002 0.0002 0.001 0.0002
Endoplasmic Reticulum 0.0061 0.0018 0.0017 0.003 0.0015 0.0013 0.0109 0.0021 0.0031 0.0017 0.0016 0.002 0.0017 0.0008 0.0012 0.0015 0.0015 0.001
Endosome 0.0267 0.0133 0.0283 0.0315 0.0317 0.0101 0.1104 0.0055 0.0312 0.0192 0.0397 0.023 0.0186 0.0175 0.0269 0.0154 0.0136 0.0138
Golgi 0.0088 0.0023 0.0044 0.006 0.0505 0.0014 0.0246 0.0007 0.0056 0.007 0.0041 0.009 0.0026 0.0015 0.003 0.0019 0.0076 0.0019
Lipid Particles 0.0091 0.003 0.0094 0.0067 0.047 0.0023 0.0482 0.0008 0.0129 0.0047 0.0027 0.003 0.0075 0.0029 0.0011 0.0035 0.0031 0.0015
Mitochondria 0.0111 0.0193 0.009 0.0495 0.049 0.0043 0.0209 0.0015 0.0181 0.0242 0.0719 0.0191 0.011 0.0237 0.0077 0.0095 0.0182 0.0236
None 0.727 0.7787 0.739 0.6938 0.417 0.7488 0.1863 0.7747 0.6412 0.6998 0.4584 0.6535 0.8554 0.8279 0.8132 0.7737 0.7785 0.7909
Nuclear Periphery 0.0064 0.0003 0.0017 0.0016 0.0022 0.0006 0.0499 0.0007 0.0015 0.0022 0.0013 0.0006 0.0078 0.0003 0.0006 0.0015 0.0015 0.0006
Nucleolus 0.0006 0.0008 0.004 0.0008 0.0006 0.0003 0.0048 0.0004 0.0007 0.0004 0.0004 0.0001 0.0008 0.0004 0.001 0.0009 0.0004 0.0004
Nucleus 0.0015 0.0009 0.0017 0.0009 0.0006 0.0007 0.009 0.0008 0.0013 0.001 0.0024 0.0006 0.0012 0.0008 0.0027 0.0018 0.0007 0.002
Peroxisomes 0.015 0.0028 0.0093 0.0111 0.0955 0.0108 0.0167 0.0004 0.0164 0.031 0.0009 0.0014 0.0013 0.002 0.0059 0.01 0.0046 0.006
Punctate Nuclear 0.0036 0.0007 0.0044 0.0073 0.017 0.0048 0.068 0.0005 0.0012 0.0006 0.0015 0.0003 0.0012 0.0004 0.0029 0.0052 0.0028 0.0023
Vacuole 0.006 0.003 0.0033 0.005 0.0014 0.002 0.0204 0.0035 0.0125 0.0708 0.005 0.0042 0.0048 0.0021 0.008 0.0067 0.0015 0.0021
Vacuole Periphery 0.0013 0.0007 0.0008 0.0019 0.0008 0.0004 0.0066 0.0005 0.0016 0.0229 0.0022 0.0008 0.0018 0.0004 0.0014 0.0014 0.0007 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.024 9.1331 17.7901 9.7525 10.7627 5.1055 9.8116 13.2444 10.7769 10.0487
Translational Efficiency 1.4383 1.063 0.3408 0.7733 0.7572 2.2089 1.0284 0.9626 1.0691 1.5651

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
249 1103 1751 1735 1695 1799 2397 1622 1944 2902 4148 3357

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 581.95 642.83 788.87 769.06 611.39 767.08 774.97 772.38 607.62 719.85 780.84 770.66
Standard Deviation 59.00 106.34 89.44 117.28 70.39 90.65 88.01 101.06 69.73 114.15 88.88 109.76
Intensity Change Log 2 — 0.143542 0.438892 0.402201 — 0.327284 0.342048 0.337218 — 0.240598 0.390088 0.369274

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000322 0.001100 0.000092 0.000756 0.000102 0.000540 0.000219 0.000299 0.000130 0.000753 0.000166 0.000535
Bud Neck 0.058032 0.057714 0.005292 0.014760 0.019943 0.059207 0.007241 0.007965 0.024822 0.058640 0.006418 0.011477
Bud Site 0.009092 0.022231 0.006779 0.040960 0.002658 0.014289 0.007796 0.017903 0.003482 0.017307 0.007366 0.029819
Cell Periphery 0.000376 0.000191 0.000084 0.000345 0.000132 0.000287 0.000103 0.000153 0.000163 0.000251 0.000095 0.000252
Cytoplasm 0.295635* 0.259847* 0.361382* 0.435793* 0.425018* 0.317532* 0.467776* 0.494697* 0.408446* 0.295607* 0.422863* 0.464253*
Cytoplasmic Foci 0.379197* 0.307522* 0.009598 0.029357 0.248051* 0.256447* 0.017566 0.039924 0.264849* 0.275860* 0.014203 0.034463
Eisosomes 0.000645 0.000199 0.000019 0.000050 0.000170 0.000281 0.000028 0.000055 0.000231 0.000250 0.000024 0.000052
Endoplasmic Reticulum 0.000838 0.001349 0.001436 0.002487 0.001414 0.000745 0.001252 0.001225 0.001340 0.000975 0.001329 0.001877
Endosome 0.023326 0.057866 0.001454 0.006913 0.010483 0.034660 0.002713 0.005805 0.012128 0.043480 0.002182 0.006378
Golgi 0.008694 0.011203 0.000063 0.003085 0.003240 0.007472 0.000238 0.001324 0.003938 0.008890 0.000164 0.002234
Lipid Particles 0.021938 0.014589 0.000800 0.001305 0.014758 0.019399 0.001052 0.002426 0.015678 0.017571 0.000946 0.001846
Mitochondria 0.044671 0.033938 0.000870 0.004330 0.010391 0.029253 0.001100 0.003205 0.014782 0.031033 0.001003 0.003787
Mitotic Spindle 0.000480 0.005408 0.003701 0.060791 0.000728 0.002607 0.003565 0.014146 0.000696 0.003672 0.003623 0.038253
None 0.001804 0.005776 0.004732 0.005131 0.003926 0.005278 0.004357 0.006364 0.003654 0.005467 0.004515 0.005727
Nuclear Periphery 0.000416 0.000571 0.002364 0.002125 0.000793 0.000653 0.002046 0.001430 0.000745 0.000622 0.002180 0.001789
Nuclear Periphery Foci 0.000153 0.000494 0.000266 0.001476 0.000252 0.000412 0.000346 0.000551 0.000240 0.000444 0.000312 0.001029
Nucleolus 0.001362 0.004466 0.000750 0.000967 0.001716 0.003600 0.000927 0.000896 0.001671 0.003929 0.000852 0.000932
Nucleus 0.087222 0.089559 0.578562* 0.283576* 0.212315* 0.152873* 0.438189* 0.304198* 0.196292* 0.128809 0.497445* 0.293540*
Peroxisomes 0.033000 0.031932 0.000329 0.002698 0.008050 0.023420 0.000685 0.001508 0.011246 0.026656 0.000535 0.002123
Vacuole 0.030606 0.087448 0.021256 0.097818 0.034744 0.067622 0.041980 0.093749 0.034214 0.075157 0.033232 0.095852
Vacuole Periphery 0.002192 0.006597 0.000171 0.005276 0.001115 0.003422 0.000821 0.002179 0.001253 0.004628 0.000546 0.003779

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.20 2.64 -2.05 0.80 -4.89 -2.50 -2.03 -3.16 1.49 -0.61 -2.34 -1.27 -5.30 1.20 -3.90
Bud Neck 0.15 7.79 6.72 12.55 -5.84 -12.55 7.63 7.29 17.69 0.11 -12.67 11.06 8.63 21.35 -4.01
Bud Site -5.08 1.21 -6.04 -1.62 -7.82 -8.44 -4.45 -6.16 0.43 -3.25 -12.24 -3.96 -11.39 -1.67 -8.29
Cell Periphery 3.88 6.31 2.06 -0.62 -2.46 -8.87 3.69 0.58 9.06 -3.04 -6.07 7.39 -0.48 2.28 -2.88
Cytoplasm 2.16 -2.19 -4.99 -12.32 -5.09 12.23 1.25 -1.22 -12.90 -2.48 14.80 3.80 0.09 -16.74 -4.26
Cytoplasmic Foci 4.50 26.49 25.38 40.65 -8.90 -1.17 42.33 37.83 41.26 -8.57 -1.78 48.68 45.07 57.91 -11.61
Eisosomes 5.41 7.72 7.30 7.65 -16.13 -6.13 14.39 11.72 14.38 -10.66 -1.02 14.75 12.65 16.11 -18.06
Endoplasmic Reticulum -1.65 -5.95 -5.68 -3.76 -2.65 2.27 -1.66 -1.45 -9.16 0.51 1.36 -2.35 -3.45 -6.96 -2.42
Endosome -7.11 5.81 5.06 17.62 -5.45 -12.45 9.87 6.82 16.31 -3.32 -17.35 11.99 8.90 23.87 -5.76
Golgi -1.46 6.48 5.16 9.07 -3.28 -5.42 8.47 5.39 8.92 -2.43 -7.33 10.62 6.30 12.33 -4.00
Lipid Particles 2.54 8.08 7.85 11.33 -5.97 -2.99 13.06 11.70 14.67 -3.10 -1.50 15.12 14.20 18.67 -4.68
Mitochondria 1.57 6.63 6.34 11.37 -4.95 -7.88 8.38 6.50 12.32 -1.96 -7.58 10.50 9.07 16.75 -4.36
Mitotic Spindle -4.14 -3.74 -10.76 -8.79 -9.02 -3.03 -4.47 -5.51 -4.22 -3.43 -4.71 -5.35 -11.86 -10.01 -9.65
None -3.21 -4.29 -4.63 1.07 -0.17 -1.66 -0.31 -1.44 0.19 -1.44 -2.39 -1.04 -1.87 0.96 -1.33
Nuclear Periphery -1.16 -14.91 -11.72 -12.47 -0.38 0.97 -11.56 -8.11 -11.13 3.91 1.01 -15.13 -11.87 -16.10 1.61
Nuclear Periphery Foci -5.56 -6.05 -3.82 -2.63 -3.11 -1.60 -4.88 -7.14 -1.06 -2.98 -2.68 -5.48 -4.67 -2.74 -3.52
Nucleolus -4.77 1.63 1.46 6.23 -0.55 -3.01 1.88 2.22 5.58 0.81 -4.42 2.21 2.34 8.13 0.37
Nucleus -0.33 -51.00 -24.86 -29.28 28.26 9.03 -33.81 -14.33 -22.52 15.96 12.04 -50.76 -20.34 -35.08 30.04
Peroxisomes 0.17 7.25 6.87 14.16 -3.32 -9.87 11.75 10.06 15.33 -1.98 -10.73 13.18 11.32 20.65 -3.74
Vacuole -11.71 -0.83 -23.04 -12.11 -24.40 -10.97 -9.57 -21.59 -14.17 -15.16 -15.68 -7.33 -31.79 -19.43 -27.16
Vacuole Periphery -6.40 6.40 -0.50 4.30 -3.51 -6.85 1.97 -0.65 3.94 -1.71 -10.26 4.51 -1.75 5.12 -3.74
Standard name
Human Ortholog
Description Protein of unknown function; null mutant exhibits a respiratory growth defect and synthetic interactions with prohibitin (phb1) and gem1; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Gep7

Gep7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gep7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available