Standard name
Human Ortholog
Description Mitochondrial glyoxalase II; catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate; GLO4 has a paralog, GLO2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.86 0.93 0.97 0.95 0.9 0.87 0.8 0.8 0.59 0.6 0.51 0.52 0.98 0.99 0.99 – 0.6 0.65 0.61 0.67 0.57 0.78 0.77 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0.07 0.06 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.11 0.1 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0 0.07 0.12 0.25 0.13 0.54 0.38 0.54 0.48 0 0 0 – 0.14 0 0.18 0.18 0.21 0.1 0.07 0.08
Nucleus 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0.06 0 0.06 0.05 0.07 0 0 0 – 0.1 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.08 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 0 0 0 0 0 0 0 0 2 0 0 0 – 7 7 4 6 13 0 2 3
Bud 0 3 0 1 4 3 9 5 10 12 23 22 0 0 0 – 3 2 2 3 8 2 5 2
Bud Neck 1 1 16 4 11 12 21 21 6 5 6 5 0 0 0 – 1 0 1 1 4 0 1 2
Bud Site 0 0 0 0 0 0 2 0 4 1 2 4 0 0 0 – 2 0 – – – – – –
Cell Periphery 0 2 3 3 3 3 0 2 2 1 2 3 2 0 1 – 7 5 0 3 6 1 2 2
Cytoplasm 166 330 440 324 330 430 392 507 235 233 245 335 107 249 276 – 121 117 147 249 269 111 196 162
Endoplasmic Reticulum 1 2 4 1 2 0 0 0 2 0 1 0 0 3 1 – 12 13 14 2 6 0 3 4
Endosome 0 4 2 3 0 8 0 10 3 7 11 11 1 0 1 – 22 18 10 9 10 2 1 2
Golgi 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 – 5 5 2 4 1 0 0 2
Mitochondria 4 16 1 9 27 58 121 81 215 148 258 310 0 1 0 – 28 7 45 65 98 14 16 17
Nucleus 12 2 1 0 7 2 2 3 1 6 5 2 2 1 2 – 4 1 0 0 1 0 2 3
Nuclear Periphery 13 0 0 0 0 0 0 0 0 1 0 0 1 0 1 – 0 0 0 0 3 0 2 0
Nucleolus 0 0 0 0 1 0 0 1 0 0 2 2 0 0 0 – 1 0 0 0 0 0 0 0
Peroxisomes 0 1 0 1 0 0 0 5 1 1 3 3 0 0 0 – 2 3 0 0 0 0 1 0
SpindlePole 1 1 2 4 7 15 15 36 11 24 23 46 0 0 0 – 20 14 3 6 17 2 6 4
Vac/Vac Membrane 1 3 6 3 5 12 2 17 11 4 13 21 0 0 1 – 16 17 5 12 9 0 5 3
Unique Cell Count 192 353 455 340 367 495 490 633 398 386 478 642 109 252 280 201 179 244 374 471 142 257 217
Labelled Cell Count 200 366 478 353 397 543 564 688 501 443 594 766 113 254 283 251 209 244 374 471 142 257 217


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.7 5.2 4.7 4.6 4.5 3.4 4.5 3.5 3.9 3.6 3.9 5.4 5.2 4.9 4.9 5.8 6.4 5.0 5.6 6.3
Std Deviation (1e-4) 0.9 1.1 1.6 2.0 2.5 2.2 1.0 1.4 1.3 1.1 1.2 1.0 2.0 1.9 1.6 1.5 1.5 1.7 1.3 2.1 2.8
Intensity Change (Log2) – – – -0.14 -0.17 -0.21 -0.62 -0.21 -0.56 -0.4 -0.51 -0.42 0.05 -0.01 -0.09 -0.1 0.15 0.3 -0.06 0.11 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – 4.4 5.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7676 0.58 0.6436 0.9515 1.1427 0.5455 3.4402 3.6566 3.7867 3.278 4.0428 3.7568 1.4166 1.7081 1.2553 1.9167 1.1159 1.4165
Actin 0.054 0.0032 0.022 0.0128 0.1132 0.0104 0.0383 0.0004 0.0317 0.0013 0.0384 0.016 0.0157 0.0007 0.0088 0.0355 0.0247 0.0038
Bud 0.001 0.0008 0.0014 0.0008 0.0004 0.0003 0.0022 0.0119 0.0013 0.0001 0.0003 0.0016 0.0007 0.0023 0.0074 0.0088 0.0023 0.0005
Bud Neck 0.0068 0.0007 0.0058 0.0003 0.0006 0.0039 0.0021 0.0003 0.0024 0.0001 0.0007 0.0103 0.0002 0.0001 0.0005 0.0027 0.0006 0.0066
Bud Periphery 0.0017 0.0023 0.0033 0.002 0.0009 0.0021 0.0073 0.0048 0.0041 0.0002 0.0008 0.0044 0.0066 0.0046 0.0108 0.0037 0.0068 0.0015
Bud Site 0.0226 0.0106 0.0148 0.003 0.0009 0.001 0.0175 0.016 0.0048 0.0001 0.0003 0.0009 0.0009 0.0103 0.0021 0.0024 0.0008 0.0003
Cell Periphery 0.001 0.0014 0.0008 0.0003 0.0002 0.0014 0.0006 0.0006 0.0007 0.0001 0.0004 0.0003 0.0004 0.0004 0.0005 0.0003 0.0002 0.0001
Cytoplasm 0.0684 0.1295 0.1265 0.1462 0.066 0.2303 0.0599 0.1075 0.0918 0.1115 0.1077 0.1566 0.0994 0.1589 0.1258 0.1688 0.1538 0.1739
Cytoplasmic Foci 0.0711 0.0438 0.0461 0.0381 0.069 0.0728 0.0589 0.0245 0.0439 0.0271 0.0129 0.0531 0.0308 0.0077 0.0234 0.0366 0.0632 0.0493
Eisosomes 0.0014 0.0006 0.0005 0.0004 0.0005 0.0003 0.0008 0.0005 0.0008 0.0002 0.0007 0.0002 0.0004 0.0002 0.0004 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0096 0.0018 0.0032 0.0014 0.0028 0.0059 0.003 0.0012 0.002 0.0008 0.0012 0.0018 0.0053 0.0012 0.0096 0.0014 0.0024 0.0014
Endosome 0.0321 0.0245 0.03 0.0123 0.023 0.0375 0.0238 0.0118 0.0189 0.0095 0.0032 0.0194 0.0271 0.0049 0.0185 0.0307 0.0288 0.0202
Golgi 0.0094 0.0047 0.0065 0.006 0.0118 0.0104 0.0045 0.0011 0.0048 0.0006 0.0014 0.0033 0.0047 0.0009 0.0079 0.0191 0.0027 0.0079
Lipid Particles 0.0221 0.0021 0.0084 0.0342 0.0283 0.0069 0.0101 0.0012 0.005 0.0005 0.0013 0.0032 0.0073 0.0002 0.014 0.0076 0.0006 0.0023
Mitochondria 0.0147 0.0115 0.0295 0.0459 0.0558 0.0242 0.0053 0.0015 0.0048 0.0012 0.0013 0.0078 0.0204 0.0044 0.0306 0.017 0.013 0.0137
None 0.6345 0.7541 0.6825 0.6354 0.5234 0.5736 0.7307 0.8057 0.7728 0.8387 0.8126 0.7005 0.7486 0.8004 0.7056 0.5856 0.6635 0.702
Nuclear Periphery 0.0131 0.0003 0.0023 0.0013 0.0038 0.0011 0.0075 0.0006 0.0008 0.0004 0.0004 0.0018 0.0174 0.0003 0.004 0.0013 0.0108 0.0004
Nucleolus 0.0015 0.0004 0.0006 0.0009 0.0003 0.0002 0.001 0.0007 0.0005 0.0001 0.0002 0.0005 0.0005 0.0001 0.0008 0.0016 0.0004 0.0002
Nucleus 0.0075 0.0005 0.0012 0.001 0.0008 0.0008 0.0065 0.0014 0.0007 0.0005 0.0004 0.0018 0.0045 0.0004 0.0015 0.0089 0.0112 0.0015
Peroxisomes 0.0109 0.0038 0.008 0.0493 0.0858 0.0112 0.0059 0.001 0.0039 0.002 0.0137 0.0106 0.0025 0.0002 0.021 0.0246 0.0079 0.0118
Punctate Nuclear 0.0098 0.0007 0.0012 0.005 0.0086 0.0027 0.0083 0.002 0.0017 0.0005 0.001 0.0031 0.0019 0.0002 0.0032 0.0124 0.0014 0.0011
Vacuole 0.0047 0.0024 0.004 0.002 0.0022 0.0022 0.0037 0.0046 0.0021 0.004 0.0011 0.0023 0.0031 0.0012 0.0025 0.0294 0.0033 0.001
Vacuole Periphery 0.0022 0.0005 0.0015 0.0013 0.0017 0.0008 0.0019 0.0005 0.0004 0.0005 0.0002 0.0006 0.0013 0.0003 0.0011 0.0017 0.0016 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.1441 5.7481 2.5592 7.2211 4.1685 7.7118 6.6837 8.6298 7.1666 10.871
Translational Efficiency 1.2397 0.9049 2.2839 0.8956 1.6322 1.2667 1.1948 1.1905 1.7122 0.9587

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1640 297 431 88 1379 2337 2887 438 3019 2634 3318 526

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 563.92 611.50 755.78 691.63 606.83 751.38 721.81 725.27 583.52 735.61 726.22 719.64
Standard Deviation 73.87 135.46 80.50 82.63 75.20 80.15 79.49 93.56 77.48 98.62 80.44 92.67
Intensity Change Log 2 — 0.116862 0.422476 0.294510 — 0.308250 0.250327 0.257226 — 0.219226 0.335813 0.275305

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000177 0.001593 0.000375 0.000419 0.000323 0.000302 0.000087 0.000498 0.000244 0.000447 0.000124 0.000484
Bud Neck 0.033939 0.035310 0.005234 0.004099 0.018327 0.042942 0.025072 0.007935 0.026808 0.042082 0.022495 0.007293
Bud Site 0.005368 0.023723 0.004082 0.021622 0.005509 0.012883 0.014444 0.024073 0.005433 0.014105 0.013098 0.023663
Cell Periphery 0.000289 0.000529 0.000157 0.000695 0.000205 0.000191 0.000169 0.000161 0.000250 0.000229 0.000167 0.000250
Cytoplasm 0.361737* 0.317263* 0.314931* 0.258442* 0.490618* 0.421751* 0.487899* 0.428586* 0.420607* 0.409969* 0.465431* 0.400121*
Cytoplasmic Foci 0.322771* 0.270673* 0.051207 0.066396 0.208925* 0.267528* 0.035949 0.040883 0.270769* 0.267883* 0.037931 0.045152
Eisosomes 0.000666 0.000416 0.000068 0.000182 0.000193 0.000301 0.000038 0.000059 0.000450 0.000314 0.000042 0.000080
Endoplasmic Reticulum 0.001926 0.002639 0.002700 0.001514 0.000440 0.000438 0.000872 0.001531 0.001247 0.000686 0.001109 0.001528
Endosome 0.011648 0.011864 0.005665 0.005767 0.008879 0.014574 0.006359 0.004638 0.010383 0.014268 0.006269 0.004827
Golgi 0.008844 0.010022 0.000341 0.000273 0.002321 0.007029 0.000521 0.000798 0.005865 0.007366 0.000498 0.000711
Lipid Particles 0.034278 0.018836 0.003146 0.005599 0.011450 0.009363 0.002311 0.001882 0.023851 0.010431 0.002419 0.002503
Mitochondria 0.022385 0.018522 0.001068 0.012084 0.007956 0.016472 0.003246 0.002624 0.015794 0.016703 0.002963 0.004207
Mitotic Spindle 0.000304 0.003403 0.002267 0.009108 0.001619 0.005498 0.006917 0.009299 0.000904 0.005262 0.006313 0.009267
None 0.009647 0.010552 0.005218 0.004385 0.004777 0.002637 0.003659 0.005893 0.007423 0.003530 0.003861 0.005640
Nuclear Periphery 0.000741 0.000509 0.001600 0.001137 0.000833 0.000376 0.001429 0.001328 0.000783 0.000391 0.001451 0.001296
Nuclear Periphery Foci 0.000293 0.000450 0.000897 0.000731 0.000220 0.000259 0.000213 0.000652 0.000260 0.000281 0.000302 0.000665
Nucleolus 0.002266 0.002521 0.001486 0.001364 0.001557 0.001340 0.002291 0.000747 0.001942 0.001473 0.002187 0.000850
Nucleus 0.138303* 0.163009* 0.550545* 0.500219* 0.187935* 0.133762* 0.349730* 0.389761* 0.160974* 0.137059* 0.375816* 0.408241*
Peroxisomes 0.015046 0.049230 0.005143 0.001119 0.008883 0.016040 0.002084 0.001481 0.012231 0.019782 0.002481 0.001420
Vacuole 0.027837 0.057872 0.043525 0.104162 0.038269 0.044857 0.055437 0.076588 0.032602 0.046325 0.053890 0.081201
Vacuole Periphery 0.001534 0.001066 0.000347 0.000682 0.000759 0.001457 0.001272 0.000584 0.001180 0.001413 0.001152 0.000600

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.98 -1.99 -0.68 2.45 0.37 0.08 1.07 -0.35 -0.65 -1.86 -1.76 1.11 -0.89 0.27 -1.78
Bud Neck -0.19 12.73 13.30 6.23 1.24 -9.63 -3.26 5.03 13.96 8.32 -6.67 2.06 10.85 15.41 8.62
Bud Site -3.86 1.30 -0.96 0.75 -1.07 -5.30 -5.64 -3.28 -1.52 -1.25 -6.92 -5.95 -3.41 -1.16 -1.49
Cell Periphery -2.10 5.93 -0.80 0.15 -1.34 0.45 1.03 1.83 2.78 0.45 1.00 2.88 1.07 0.57 -0.85
Cytoplasm 2.72 4.50 4.46 2.46 2.07 7.43 0.89 6.93 2.63 6.76 1.40 -5.55 4.58 3.77 7.43
Cytoplasmic Foci 3.67 38.62 23.79 12.98 -0.78 -8.20 31.72 28.08 40.43 0.26 0.55 55.88 45.79 41.81 -0.33
Eisosomes 3.29 9.44 7.81 5.17 -4.88 -5.09 12.10 9.17 13.23 -7.10 3.57 11.73 10.42 13.77 -8.53
Endoplasmic Reticulum -0.66 -2.63 0.40 0.86 2.98 -0.00 -6.72 -9.56 -9.65 -7.33 2.49 0.09 -4.18 -7.72 -5.81
Endosome -0.07 4.22 4.70 3.37 0.30 -5.44 2.67 4.35 10.13 2.69 -4.94 6.16 7.20 10.79 2.66
Golgi -0.37 13.30 13.50 4.55 0.82 -7.02 5.04 4.24 10.05 0.01 -2.13 13.35 12.12 11.15 0.10
Lipid Particles 3.52 17.45 14.41 3.58 -1.04 1.69 8.17 8.58 11.58 0.86 10.38 19.30 19.31 10.84 -0.01
Mitochondria 0.95 12.00 1.93 1.16 -0.90 -4.81 3.49 3.66 8.84 1.18 -0.52 10.73 6.84 7.07 0.08
Mitotic Spindle -3.36 -1.97 -1.23 -0.66 -0.79 -3.32 -4.86 -2.20 -0.55 0.22 -4.66 -6.19 -2.78 -0.74 -0.08
None -0.20 2.95 2.71 1.85 0.44 3.59 1.98 -0.20 -2.23 -1.33 4.49 4.54 2.25 -1.31 -1.15
Nuclear Periphery 1.03 -4.40 -2.46 -3.73 0.79 1.13 -1.78 -2.78 -9.13 -2.66 1.69 -3.54 -4.65 -9.67 -2.27
Nuclear Periphery Foci -2.51 -4.85 -2.73 -1.37 1.49 -0.34 -0.63 -6.17 -3.89 -5.27 -0.24 -1.93 -6.37 -4.09 -4.57
Nucleolus -0.41 1.92 2.51 2.01 0.75 1.08 -2.77 4.23 4.09 7.62 2.10 -1.22 5.77 4.30 7.41
Nucleus -1.71 -28.00 -8.96 -7.85 2.82 9.01 -23.19 -14.21 -18.54 -3.18 5.45 -38.01 -18.47 -20.25 -2.10
Peroxisomes -4.81 5.00 11.80 6.97 2.42 -5.22 6.28 7.32 16.21 3.07 -5.40 11.31 13.47 16.43 3.96
Vacuole -4.31 -4.91 -5.66 -4.31 -4.67 -2.25 -6.97 -9.76 -9.23 -7.61 -6.96 -11.70 -11.78 -10.14 -8.86
Vacuole Periphery 1.50 9.17 3.53 1.35 -0.57 -5.38 -2.52 1.64 5.01 3.28 -1.97 0.44 4.19 5.33 3.18
Standard name
Human Ortholog
Description Mitochondrial glyoxalase II; catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate; GLO4 has a paralog, GLO2, that arose from the whole genome duplication
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Glo4

Glo4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Glo4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available