Standard name
Human Ortholog
Description (SGD) Tetrameric phosphoglycerate mutase; mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.06 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0.07 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 1.0 0.99 0.98 0.98 0.97 0.95 0.97 0.94 0.96 0.97 0.98 0.98 0.97 0.96 1.0 0.98 0.98 0.97 0.98 0.97 0.95 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Bud 0 1 0 1 1 0 1 4 2 1 0 0 0 0 0 0 0 0 0 0 0 0 1 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 1 6 5 10 4 10 9 6 16 8 13 5 8 14 0 3 2 0 0 0 0 3 3
Cytoplasm 54 178 170 198 241 260 331 327 278 270 197 265 122 235 194 129 177 171 80 182 162 192 221 207
Endoplasmic Reticulum 0 0 0 0 0 0 3 10 1 4 1 4 1 0 0 10 12 12 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 2 0 1 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0
Nucleus 0 1 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0 0 0 1 0 1 0 2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1
Unique Cell Count 54 180 170 200 245 265 341 344 287 287 205 274 124 239 201 135 177 174 82 188 166 199 232 223
Labelled Cell Count 57 181 176 205 252 267 345 351 289 291 207 282 128 244 208 147 192 186 82 188 166 199 232 223


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 487.4 291.9 399.7 387.7 370.3 323.1 291.4 253.2 234.9 243.9 236.3 229.4 435.9 441.9 466.9 289.3 367.6 363.9 395.5 433.3 451.2
Std Deviation (1e-4) 72.5 66.6 81.8 91.8 81.4 84.3 68.6 71.2 64.9 65.2 70.0 54.4 106.9 99.2 95.3 106.4 123.4 112.9 128.3 138.9 154.0
Intensity Change (Log2) – – – -0.04 -0.11 -0.31 -0.46 -0.66 -0.77 -0.71 -0.76 -0.8 0.13 0.14 0.22 -0.47 -0.12 -0.14 -0.02 0.12 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.6 0.3 0 -0.4 0 0 1.0 0.2 0.6 0 -0.1 1.5 0 0 0
Cytoplasm -1.3 -1.7 -1.8 -2.3 -2.9 -2.3 -3.2 -2.6 -2.4 -1.7 -1.7 -2.5 -2.8 0 -1.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 3.5 3.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 360.8327 461.0806 398.8721 383.3666 422.0887 421.3475 339.6436 457.412 368.8495 270.9394 331.0743 183.4156 363.4306 442.5893 378.6269 383.3171 473.755 324.2793
Actin 0.0066 0.0002 0.0018 0.0001 0.0001 0.0002 0.0033 0.0001 0.0033 0.0043 0.0026 0.0024 0.0008 0.0002 0.0014 0.0002 0.0001 0.0009
Bud 0.0006 0.0002 0.0005 0.0001 0.0001 0.0006 0.0025 0.0001 0.0018 0.0122 0.004 0.0088 0.0009 0.0003 0.0005 0.0001 0.0001 0.0002
Bud Neck 0.0003 0.0002 0.0005 0.0002 0.0002 0.0006 0.0003 0.0001 0.0003 0.0011 0.0009 0.0011 0.0002 0.0001 0.0003 0.0005 0.0003 0.0006
Bud Periphery 0.0006 0 0.0002 0 0 0.0004 0.001 0 0.0006 0.0054 0.0023 0.0015 0.0002 0 0.0002 0 0 0
Bud Site 0.001 0.0001 0.0002 0 0 0.0001 0.0005 0.0001 0.001 0.0023 0.0005 0.0014 0.0001 0.0001 0.0003 0 0 0
Cell Periphery 0.0047 0.0013 0.0011 0.0001 0 0.0003 0.0006 0.0001 0.001 0.0055 0.0015 0.001 0.0008 0.0002 0.0007 0.0001 0 0.0001
Cytoplasm 0.9087 0.9803 0.9397 0.9899 0.9937 0.983 0.9298 0.9973 0.9292 0.7074 0.9095 0.6454 0.9599 0.9955 0.9727 0.9927 0.9934 0.8376
Cytoplasmic Foci 0.0089 0.0011 0.0097 0.0002 0.0005 0.0003 0.0068 0.0001 0.0155 0.0279 0.0036 0.1071 0.0003 0.0001 0.0008 0.0002 0.0001 0.0002
Eisosomes 0.0005 0 0.0003 0 0 0 0.0002 0 0.0004 0.0041 0.0006 0.0006 0.0001 0 0.0004 0 0 0
Endoplasmic Reticulum 0.0015 0.0003 0.0006 0.0001 0.0001 0.0004 0.001 0 0.0003 0.0032 0.0027 0.0006 0.0008 0.0002 0.0008 0.0001 0 0.0005
Endosome 0.0033 0 0.0014 0 0.0002 0.0001 0.0006 0 0.0012 0.0014 0.0046 0.0017 0.0001 0.0001 0.0002 0 0 0.0002
Golgi 0.0008 0 0.0038 0 0 0 0.0001 0 0.0004 0.0005 0.0009 0.0008 0 0.0001 0.0001 0 0 0
Lipid Particles 0.0024 0.0001 0.0015 0 0 0 0.0006 0 0.0016 0.0066 0.0019 0.0093 0.0001 0 0.0003 0 0 0
Mitochondria 0.0137 0.0017 0.0059 0.0001 0.0002 0.0007 0.0032 0 0.0047 0.0312 0.0089 0.034 0.0018 0.0001 0.0013 0.0001 0.0001 0.0005
None 0.0055 0.0013 0.0072 0.0002 0.0003 0.001 0.0053 0.0001 0.014 0.047 0.0186 0.0582 0.0017 0.0001 0.0053 0.0002 0.0001 0.0004
Nuclear Periphery 0.0056 0.0051 0.003 0.0029 0.001 0.0023 0.0148 0.0002 0.0038 0.0238 0.0046 0.004 0.012 0.0005 0.0044 0.0011 0.0007 0.0416
Nucleolus 0.0129 0.0018 0.0079 0 0 0.0002 0.0116 0 0.0072 0.0405 0.0027 0.0532 0.0006 0 0.0005 0 0 0.0003
Nucleus 0.0117 0.0047 0.0079 0.0052 0.0027 0.0072 0.0143 0.0015 0.0071 0.0453 0.0145 0.0246 0.0177 0.002 0.0078 0.0042 0.0049 0.1142
Peroxisomes 0.0012 0.0001 0.0004 0 0 0 0.0002 0 0.0004 0.0017 0.0003 0.0017 0.0001 0 0.0001 0 0 0
Punctate Nuclear 0.0005 0 0.0007 0.0001 0 0.0001 0.0008 0 0.0006 0.0048 0.0003 0.0361 0.0002 0 0.0002 0 0 0.0017
Vacuole 0.0059 0.001 0.0039 0.0006 0.0005 0.0018 0.0014 0.0001 0.0033 0.0151 0.0084 0.0036 0.001 0.0003 0.0011 0.0002 0.0001 0.0005
Vacuole Periphery 0.0033 0.0004 0.0017 0.0003 0.0004 0.0007 0.0009 0 0.0023 0.0088 0.0059 0.0029 0.0005 0.0001 0.0005 0.0001 0 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6089.4874 6089.2819 6771.3703 5803.3027 6423.3329 6607.8681 6167.9257 6138.6598 7293.6888 6051.2244
Translational Efficiency 1.5345 1.5101 1.494 1.686 1.6429 1.4359 1.6908 2.0715 1.7082 2.0678

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘


Micrographs

Cell Count

Number of cells analysed for each strain. R1, R2: biological replicates; R1 & R2: both replicates combined.

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
202 329 2100 1417 1308 1996 270 1719 1510 2325 2370 3136

Protein Abundance

Mean and standard deviation of cell GFP intensity, and intensity change (log2) of each mutant relative to wild type.

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 19553.67 25937.04 33770.68 34021.71 21586.79 26986.80 42469.20 35048.86 21314.81 26838.25 34761.65 34584.74
Standard Deviation 4064.90 5508.92 5857.28 8056.88 6061.38 5166.25 6093.34 7429.13 5874.93 5228.92 6501.31 7736.01
Intensity Change Log 2 — 0.407574 0.788332 0.799016 — 0.322105 0.976268 0.699219 — 0.363360 0.889988 0.747513

Localization Score (DeepLoc)

Localization score for each compartment. Values highlighted in green denote significant localization scores (marked * in the plot).

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000123 0.000095 0.000036 0.000179 0.000031 0.000043 0.000144 0.000128 0.000043 0.000050 0.000048 0.000151
Bud Neck 0.001197 0.000949 0.000283 0.001029 0.000204 0.000231 0.003370 0.000544 0.000337 0.000332 0.000635 0.000763
Bud Site 0.004534 0.003133 0.001196 0.003436 0.000558 0.000683 0.000915 0.002830 0.001090 0.001029 0.001164 0.003104
Cell Periphery 0.042827 0.024609 0.004590 0.025244 0.004840 0.002872 0.066028 0.012898 0.009922 0.005948 0.011589 0.018477
Cytoplasm 0.712426* 0.801672* 0.903829* 0.745397* 0.877843* 0.910769* 0.502284* 0.839780* 0.855714* 0.895331* 0.858083* 0.797133*
Cytoplasmic Foci 0.017938 0.011330 0.001299 0.003381 0.003283 0.004543 0.010635 0.002069 0.005243 0.005503 0.002363 0.002662
Eisosomes 0.000004 0.000003 0.000001 0.000006 0.000001 0.000001 0.000023 0.000003 0.000002 0.000001 0.000003 0.000004
Endoplasmic Reticulum 0.000224 0.000045 0.000054 0.000052 0.000020 0.000019 0.000029 0.000030 0.000047 0.000023 0.000051 0.000040
Endosome 0.000452 0.000102 0.000058 0.000051 0.000030 0.000050 0.000047 0.000024 0.000086 0.000058 0.000057 0.000036
Golgi 0.032931 0.006621 0.001869 0.000791 0.004495 0.002962 0.002101 0.000678 0.008299 0.003480 0.001895 0.000729
Lipid Particles 0.020785 0.014076 0.003551 0.009029 0.002048 0.002422 0.018409 0.004095 0.004554 0.004071 0.005244 0.006324
Mitochondria 0.000115 0.000107 0.000075 0.000254 0.000099 0.000104 0.000073 0.000168 0.000101 0.000104 0.000075 0.000207
Mitotic Spindle 0.001559 0.000222 0.000050 0.000204 0.000109 0.000180 0.000052 0.000048 0.000303 0.000186 0.000050 0.000118
None 0.005392 0.006913 0.001784 0.006851 0.011736 0.007692 0.001519 0.007931 0.010887 0.007582 0.001754 0.007443
Nuclear Periphery 0.000921 0.000542 0.000120 0.000214 0.000261 0.000168 0.001417 0.000146 0.000349 0.000221 0.000268 0.000177
Nuclear Periphery Foci 0.010172 0.003617 0.001137 0.001367 0.001786 0.001667 0.002745 0.001043 0.002908 0.001943 0.001320 0.001189
Nucleolus 0.000083 0.000079 0.000055 0.000130 0.000048 0.000040 0.000075 0.000088 0.000052 0.000046 0.000057 0.000107
Nucleus 0.055792 0.060143 0.018956 0.044218 0.073098 0.053987 0.091675 0.045846 0.070783 0.054858 0.027241 0.045111
Peroxisomes 0.000031 0.000018 0.000017 0.000037 0.000003 0.000009 0.000006 0.000019 0.000007 0.000010 0.000016 0.000027
Vacuole 0.071395 0.061344 0.058135 0.156581 0.016087 0.009621 0.295179* 0.080811 0.023486 0.016940 0.085140 0.115048
Vacuole Periphery 0.021100 0.004379 0.002905 0.001550 0.003421 0.001938 0.003274 0.000823 0.005786 0.002284 0.002947 0.001151

Localization Changes (T-score)

Change in localization of each mutant relative to its control (e.g. UBP2_WT: ubp2Δ vs wild type). Values highlighted in yellow (increase) or blue (decrease) denote significant changes (marked * in the plot).

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.27 3.40 -1.62 -4.38 -9.07 -1.00 -10.78 -6.89 -3.09 1.59 -0.75 -1.88 -9.61 -4.46 -9.23
Bud Neck 0.73 3.09 0.18 -0.98 -7.84 -1.37 -10.89 -9.20 -7.52 9.42 -0.34 -6.46 -8.32 -8.71 -1.95
Bud Site 1.29 3.37 1.13 -0.41 -4.92 -0.57 -2.60 -5.11 -5.04 -3.97 0.55 -0.35 -5.69 -6.46 -5.96
Cell Periphery 2.83 6.78 3.36 0.44 -11.42 3.33 -11.08 -7.61 -10.04 9.42 3.68 -0.95 -5.97 -11.19 -5.47
Cytoplasm -3.53 -9.46 -1.66 3.27 19.28 -5.36 20.90 7.74 14.21 -18.11 -5.95 1.59 10.60 19.53 9.16
Cytoplasmic Foci 1.96 6.38 5.41 5.09 -3.69 -2.85 -6.27 1.78 4.55 6.68 -0.74 4.32 3.93 5.12 -0.30
Eisosomes 1.08 5.23 -1.59 -3.11 -9.61 2.22 -9.09 -3.84 -5.37 8.28 2.32 -4.28 -7.01 -9.11 -2.59
Endoplasmic Reticulum 3.49 3.31 3.34 -0.48 0.31 0.59 -1.17 -0.75 -1.42 0.17 3.06 -0.27 1.10 -2.51 1.74
Endosome 2.67 3.01 3.07 2.47 0.87 -1.95 -3.30 0.89 2.63 5.36 1.19 1.31 2.59 2.20 3.85
Golgi 4.97 5.98 6.24 6.42 7.30 2.01 3.46 5.77 7.64 5.95 4.78 6.67 8.18 9.50 9.06
Lipid Particles 1.61 5.07 3.43 1.36 -5.70 -0.97 -8.86 -5.09 -2.80 7.46 0.42 -1.79 -3.06 -3.19 -1.57
Mitochondria -0.08 1.97 -2.03 -1.62 -2.65 -0.64 1.50 -1.89 -1.52 -2.24 -0.59 2.73 -2.91 -2.51 -3.52
Mitotic Spindle 3.46 3.96 3.35 0.29 -0.97 -1.71 1.94 1.85 3.36 0.11 1.59 4.33 2.13 1.09 -0.95
None -1.39 5.12 -0.57 0.38 -3.34 3.49 9.56 2.76 0.43 -4.49 3.29 9.62 3.29 0.96 -5.33
Nuclear Periphery 2.33 5.97 5.31 4.39 -4.50 2.28 -10.03 2.77 0.34 11.00 3.05 0.98 4.43 1.45 5.05
Nuclear Periphery Foci 3.63 5.29 5.14 4.45 -1.76 0.37 -2.94 2.56 2.76 6.62 2.48 4.19 4.83 3.47 1.70
Nucleolus -0.16 2.29 -2.79 -1.83 -6.32 0.74 -3.91 -3.98 -4.65 -0.98 0.58 -1.17 -6.14 -6.63 -6.52
Nucleus -0.57 5.60 1.19 2.29 -11.59 4.55 -4.39 5.68 1.46 7.15 4.07 11.70 5.84 2.07 -9.08
Peroxisomes 1.29 1.57 -0.18 -1.82 -2.23 -2.53 -3.23 -4.65 -2.36 -3.41 -1.34 -3.99 -4.30 -3.41 -2.35
Vacuole 0.73 1.22 -7.10 -9.06 -14.25 2.85 -16.47 -17.54 -20.75 11.96 2.30 -16.32 -24.13 -27.98 -5.61
Vacuole Periphery 3.43 3.78 4.14 3.71 5.08 2.52 0.77 5.82 3.16 6.31 4.16 3.47 6.26 3.57 7.78
Standard name
Human Ortholog
Description (SGD) Tetrameric phosphoglycerate mutase; mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis
Localization
Cell Percentages cytoplasm (90%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Gpm1

Gpm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)