Standard name | |
---|---|
Human Ortholog | |
Description | Disaggregase; heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responsive to stresses including: heat, ethanol, and sodium arsenite; involved in [PSI+] propagation; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress; potentiated Hsp104p variants decrease TDP-43 proteotoxicity by eliminating its cytoplasmic aggregation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 1 | 1 | 1 | 0 | 4 | – | 3 | 1 | 8 | 4 | 10 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 25 | 17 |
Bud | 1 | 1 | 4 | 0 | 7 | 3 | 8 | – | 5 | 8 | 3 | 5 | 14 | 1 | 3 | 3 | 1 | 2 | 2 | 6 | 6 | 4 | 23 | 38 |
Bud Neck | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 10 | 8 | 12 | 19 | 25 | – | 19 | 11 | 23 | 30 | 2 | 5 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 2 |
Cytoplasm | 347 | 485 | 480 | 248 | 379 | 293 | 374 | – | 241 | 254 | 248 | 263 | 359 | 384 | 486 | 305 | 215 | 144 | 338 | 505 | 497 | 340 | 257 | 256 |
Endoplasmic Reticulum | 5 | 6 | 8 | 9 | 23 | 40 | 48 | – | 42 | 39 | 35 | 39 | 26 | 29 | 21 | 12 | 5 | 10 | 1 | 6 | 6 | 48 | 36 | 42 |
Endosome | 0 | 2 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 2 | 2 | 3 | 20 | 17 |
Golgi | 0 | 0 | 4 | 7 | 7 | 3 | 3 | – | 6 | 6 | 10 | 6 | 44 | 45 | 5 | 24 | 15 | 9 | 0 | 1 | 3 | 6 | 27 | 30 |
Mitochondria | 0 | 1 | 4 | 3 | 6 | 2 | 0 | – | 1 | 1 | 2 | 0 | 2 | 2 | 5 | 6 | 3 | 4 | 0 | 0 | 0 | 3 | 4 | 11 |
Nucleus | 0 | 0 | 1 | 0 | 0 | 1 | 0 | – | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 14 | 3 | 3 | 1 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 1 | 4 | 5 |
Nucleolus | 1 | 1 | 4 | 5 | 9 | 4 | 5 | – | 2 | 6 | 5 | 2 | 4 | 9 | 12 | 16 | 8 | 16 | 0 | 0 | 2 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 12 | 14 |
SpindlePole | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 0 | 13 | 17 |
Vac/Vac Membrane | 11 | 20 | 22 | 28 | 14 | 5 | 2 | – | 5 | 8 | 8 | 13 | 14 | 5 | 6 | 26 | 20 | 19 | 5 | 24 | 9 | 17 | 12 | 13 |
Unique Cell Count | 363 | 511 | 518 | 293 | 435 | 357 | 449 | 307 | 319 | 321 | 347 | 450 | 468 | 526 | 362 | 253 | 182 | 371 | 573 | 543 | 443 | 451 | 481 | |
Labelled Cell Count | 366 | 520 | 538 | 309 | 458 | 370 | 470 | 325 | 334 | 343 | 363 | 476 | 482 | 539 | 393 | 268 | 206 | 371 | 573 | 543 | 443 | 451 | 481 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 34.8 | 34.8 | 37.6 | 39.9 | 54.2 | 75.8 | 77.7 | – | 96.2 | 89.9 | 85.1 | 105.0 | 45.3 | 65.9 | 80.9 | 86.1 | 102.9 | 83.0 | 29.2 | 37.8 | 48.9 |
Std Deviation (1e-4) | 10.2 | 10.4 | 16.7 | 24.0 | 24.4 | 38.6 | 29.1 | – | 46.9 | 43.3 | 36.8 | 55.2 | 18.6 | 24.8 | 28.5 | 55.6 | 64.5 | 55.5 | 11.4 | 16.9 | 21.5 |
Intensity Change (Log2) | – | – | – | 0.09 | 0.53 | 1.01 | 1.05 | – | 1.36 | 1.26 | 1.18 | 1.48 | 0.27 | 0.81 | 1.11 | 1.2 | 1.45 | 1.14 | -0.36 | 0.01 | 0.38 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 3.0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 1.2 | 0 | 1.4 | – | 0 | 0 | 0 | 0 | 2.7 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.7 | 0.8 | 2.8 | 3.0 | – | 3.2 | 1.4 | 3.8 | 4.6 | -2.1 | -1.1 | -2.8 | 0 | 0 | 0 |
Cytoplasm | -3.6 | -2.9 | -4.8 | -4.5 | – | -5.9 | -5.6 | -6.4 | -7.0 | -5.9 | -5.1 | -0.2 | -4.0 | -3.4 | -5.1 |
Endoplasmic Reticulum | 1.5 | 3.2 | 6.2 | 6.1 | – | 7.1 | 6.5 | 6.0 | 6.2 | 3.6 | 3.8 | 2.4 | 1.7 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 1.2 | 0 | 0 | – | 0 | 0 | 2.6 | 0 | 6.4 | 6.4 | 0 | 4.9 | 4.3 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 1.7 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1.6 | 2.0 | 3.6 | 0 | 5.6 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 3.0 | -0.8 | -2.4 | -3.8 | – | -2.0 | -1.3 | -1.3 | -0.4 | -0.9 | -3.1 | -3.1 | 1.9 | 2.1 | 3.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 29.4594 | 50.1393 | 42.6709 | 47.5835 | 39.8328 | 42.887 | 34.2338 | 48.8107 | 42.6155 | 43.9329 | 44.9896 | 49.3715 | 42.6135 | 57.8692 | 50.0723 | 51.1581 | 47.1787 | 51.0277 |
Actin | 0.021 | 0.0001 | 0.0071 | 0.0005 | 0.0016 | 0.0011 | 0.0255 | 0.0002 | 0.0107 | 0.002 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0.0002 | 0.0337 | 0.0002 | 0.0002 |
Bud | 0.001 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0002 | 0.0024 | 0.0022 | 0.0005 | 0.0003 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Bud Neck | 0.0006 | 0.0002 | 0.0007 | 0.0004 | 0.0003 | 0.0007 | 0.001 | 0.0001 | 0.0005 | 0.0004 | 0.0005 | 0.0006 | 0.0002 | 0.0002 | 0.0002 | 0.0006 | 0.0004 | 0.0005 |
Bud Periphery | 0.0013 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0032 | 0.0003 | 0.0002 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0 |
Bud Site | 0.0019 | 0.0001 | 0.0007 | 0.0002 | 0.0002 | 0 | 0.002 | 0.0004 | 0.0011 | 0.0001 | 0 | 0 | 0.0003 | 0.0001 | 0 | 0.0001 | 0 | 0 |
Cell Periphery | 0.0004 | 0 | 0.0001 | 0.0002 | 0.0004 | 0 | 0.0008 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0 | 0 | 0 | 0.0001 |
Cytoplasm | 0.7495 | 0.9815 | 0.9339 | 0.9437 | 0.9276 | 0.9178 | 0.8211 | 0.9635 | 0.9067 | 0.8636 | 0.9081 | 0.975 | 0.9138 | 0.9757 | 0.9832 | 0.9312 | 0.9871 | 0.9653 |
Cytoplasmic Foci | 0.0233 | 0.002 | 0.0049 | 0.0009 | 0.0208 | 0.0013 | 0.017 | 0.0082 | 0.012 | 0.0032 | 0.0555 | 0.0008 | 0.0052 | 0.0029 | 0.001 | 0.0017 | 0.0005 | 0.0008 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0004 | 0 | 0 |
Endoplasmic Reticulum | 0.0117 | 0.0003 | 0.0007 | 0.0004 | 0.01 | 0.0029 | 0.0084 | 0.0018 | 0.0055 | 0.0005 | 0.0007 | 0.0034 | 0.0037 | 0.0017 | 0.0004 | 0.0012 | 0.0006 | 0.0023 |
Endosome | 0.0204 | 0.0003 | 0.0016 | 0.0005 | 0.0019 | 0.0051 | 0.0122 | 0.0004 | 0.0057 | 0.0056 | 0.0002 | 0.0008 | 0.0036 | 0.0007 | 0.0004 | 0.0028 | 0.0003 | 0.0005 |
Golgi | 0.0054 | 0 | 0.0013 | 0.0001 | 0.0005 | 0.0006 | 0.0039 | 0 | 0.0018 | 0.0177 | 0 | 0 | 0.0004 | 0 | 0 | 0.0022 | 0 | 0 |
Lipid Particles | 0.0244 | 0.0001 | 0.0109 | 0 | 0.0036 | 0.0009 | 0.0059 | 0 | 0.0034 | 0.0006 | 0.0001 | 0 | 0.0011 | 0.0003 | 0 | 0.0031 | 0 | 0 |
Mitochondria | 0.0124 | 0.0001 | 0.0058 | 0.0001 | 0.0016 | 0.0005 | 0.0134 | 0.0002 | 0.0017 | 0.0071 | 0 | 0.0001 | 0.0037 | 0 | 0.0001 | 0.0006 | 0 | 0.0001 |
None | 0.0118 | 0.0004 | 0.0008 | 0.0002 | 0.0147 | 0.0004 | 0.0027 | 0.0005 | 0.0011 | 0.0002 | 0.0001 | 0.0002 | 0.0077 | 0.0002 | 0.0005 | 0.0032 | 0.0002 | 0.0005 |
Nuclear Periphery | 0.0334 | 0.0016 | 0.0053 | 0.004 | 0.006 | 0.0245 | 0.0153 | 0.002 | 0.0089 | 0.007 | 0.0052 | 0.0035 | 0.0101 | 0.0019 | 0.0017 | 0.0036 | 0.0016 | 0.0055 |
Nucleolus | 0.0015 | 0.0012 | 0.0012 | 0 | 0.0004 | 0.0002 | 0.0019 | 0.0001 | 0.0003 | 0.0001 | 0 | 0 | 0.0029 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0008 |
Nucleus | 0.0471 | 0.0097 | 0.0184 | 0.0437 | 0.0049 | 0.0364 | 0.0367 | 0.0178 | 0.0313 | 0.0874 | 0.0281 | 0.0138 | 0.0383 | 0.0148 | 0.0109 | 0.0101 | 0.0076 | 0.021 |
Peroxisomes | 0.0028 | 0 | 0.0023 | 0 | 0.0002 | 0.0002 | 0.0067 | 0 | 0.0018 | 0.002 | 0 | 0 | 0.0002 | 0 | 0 | 0.0022 | 0 | 0 |
Punctate Nuclear | 0.013 | 0.0012 | 0.0012 | 0.0001 | 0.0017 | 0.0022 | 0.0048 | 0.0004 | 0.0018 | 0.0006 | 0.0007 | 0.0001 | 0.0032 | 0.0002 | 0.0002 | 0.0017 | 0.0001 | 0.0008 |
Vacuole | 0.0119 | 0.0007 | 0.0016 | 0.004 | 0.0021 | 0.0032 | 0.0094 | 0.0014 | 0.0033 | 0.0012 | 0.0005 | 0.0012 | 0.0029 | 0.0006 | 0.0006 | 0.0008 | 0.0011 | 0.0012 |
Vacuole Periphery | 0.0048 | 0.0002 | 0.0007 | 0.0008 | 0.001 | 0.0014 | 0.0055 | 0.0002 | 0.0014 | 0.0004 | 0.0001 | 0.0003 | 0.0012 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 101.3578 | 115.3847 | 130.9804 | 226.5621 | 126.9557 | 138.3041 | 185.6039 | 262.8822 | 294.867 | 160.456 |
Translational Efficiency | 1.9003 | 2.1958 | 1.9935 | 1.8803 | 2.0131 | 2.0954 | 1.747 | 1.5778 | 1.8426 | 1.7492 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1524 | 1326 | 159 | 1701 | 1582 | 1555 | 1289 | 89 | 3106 | 2881 | 1448 | 1790 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 3045.21 | 2723.61 | 14674.80 | 10523.48 | 2049.68 | 2341.73 | 5015.96 | 13524.77 | 2538.15 | 2517.49 | 6076.56 | 10672.71 |
Standard Deviation | 816.78 | 1281.31 | 4024.70 | 4308.89 | 709.52 | 1037.62 | 2001.87 | 5593.26 | 911.83 | 1171.74 | 3803.35 | 4429.95 |
Intensity Change Log 2 | — | -0.161022 | 2.268727 | 1.788998 | — | 0.192176 | 1.291127 | 2.722133 | — | -0.008392 | 1.950396 | 2.238809 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000209 | 0.001282 | 0.000409 | 0.001141 | 0.000656 | 0.000759 | 0.000311 | 0.020342 | 0.000437 | 0.000999 | 0.000321 | 0.002096 |
Bud Neck | 0.000525 | 0.004542 | 0.009253 | 0.006127 | 0.002395 | 0.003392 | 0.005617 | 0.021871 | 0.001477 | 0.003921 | 0.006016 | 0.006909 |
Bud Site | 0.006654 | 0.013224 | 0.004407 | 0.035071 | 0.009469 | 0.012127 | 0.010394 | 0.052673 | 0.008088 | 0.012632 | 0.009737 | 0.035946 |
Cell Periphery | 0.001607 | 0.001539 | 0.093481 | 0.009109 | 0.001186 | 0.000966 | 0.006246 | 0.005998 | 0.001393 | 0.001229 | 0.015825 | 0.008955 |
Cytoplasm | 0.900025* | 0.764001* | 0.241075* | 0.500507* | 0.822346* | 0.757388* | 0.673444* | 0.140322* | 0.860460* | 0.760431* | 0.625967* | 0.482598* |
Cytoplasmic Foci | 0.022897 | 0.041188 | 0.057591 | 0.141276 | 0.017854 | 0.035735 | 0.037643 | 0.240357* | 0.020329 | 0.038245 | 0.039834 | 0.146202 |
Eisosomes | 0.000001 | 0.000006 | 0.000037 | 0.000016 | 0.000003 | 0.000013 | 0.000006 | 0.000180 | 0.000002 | 0.000010 | 0.000009 | 0.000024 |
Endoplasmic Reticulum | 0.000378 | 0.000907 | 0.000226 | 0.000221 | 0.002190 | 0.000982 | 0.000404 | 0.000546 | 0.001300 | 0.000948 | 0.000384 | 0.000237 |
Endosome | 0.000695 | 0.001408 | 0.000688 | 0.005916 | 0.001828 | 0.002144 | 0.002163 | 0.006077 | 0.001272 | 0.001805 | 0.002001 | 0.005924 |
Golgi | 0.018693 | 0.008876 | 0.031201 | 0.015644 | 0.012600 | 0.011759 | 0.013864 | 0.032785 | 0.015590 | 0.010432 | 0.015768 | 0.016496 |
Lipid Particles | 0.006021 | 0.008022 | 0.050636 | 0.081362 | 0.006592 | 0.007702 | 0.021621 | 0.167061* | 0.006312 | 0.007849 | 0.024807 | 0.085623 |
Mitochondria | 0.000166 | 0.000606 | 0.001289 | 0.000736 | 0.000435 | 0.001984 | 0.000728 | 0.000508 | 0.000303 | 0.001350 | 0.000789 | 0.000725 |
Mitotic Spindle | 0.003465 | 0.011239 | 0.003961 | 0.086462 | 0.008055 | 0.019955 | 0.026625 | 0.055192 | 0.005803 | 0.015943 | 0.024136 | 0.084908 |
None | 0.003680 | 0.006226 | 0.001826 | 0.003964 | 0.006296 | 0.008755 | 0.005884 | 0.000620 | 0.005012 | 0.007591 | 0.005438 | 0.003798 |
Nuclear Periphery | 0.000086 | 0.000459 | 0.008082 | 0.000463 | 0.000392 | 0.000438 | 0.000952 | 0.001030 | 0.000242 | 0.000448 | 0.001735 | 0.000491 |
Nuclear Periphery Foci | 0.006373 | 0.015138 | 0.015258 | 0.009216 | 0.014310 | 0.016051 | 0.013549 | 0.018374 | 0.010416 | 0.015631 | 0.013737 | 0.009672 |
Nucleolus | 0.000047 | 0.000470 | 0.000292 | 0.000198 | 0.000237 | 0.000837 | 0.000389 | 0.001073 | 0.000144 | 0.000668 | 0.000378 | 0.000241 |
Nucleus | 0.010468 | 0.103110 | 0.074405 | 0.015459 | 0.070827 | 0.097786 | 0.061261 | 0.012906 | 0.041211 | 0.100236 | 0.062704 | 0.015332 |
Peroxisomes | 0.000053 | 0.000241 | 0.000227 | 0.001337 | 0.000058 | 0.000319 | 0.000218 | 0.000619 | 0.000056 | 0.000283 | 0.000219 | 0.001301 |
Vacuole | 0.008103 | 0.014386 | 0.352999* | 0.079022 | 0.016862 | 0.017434 | 0.109725 | 0.209679* | 0.012564 | 0.016031 | 0.136438 | 0.085519 |
Vacuole Periphery | 0.009853 | 0.003130 | 0.052657 | 0.006754 | 0.005411 | 0.003475 | 0.008957 | 0.011788 | 0.007591 | 0.003316 | 0.013756 | 0.007004 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.48 | -1.77 | -5.36 | 0.51 | -4.54 | -0.11 | 1.57 | -1.46 | -1.45 | -1.53 | -2.08 | 1.02 | -2.74 | -1.22 | -3.43 |
Bud Neck | -7.08 | -8.42 | -18.25 | -3.12 | 2.20 | -2.67 | -7.60 | -4.88 | -4.51 | -3.47 | -7.29 | -12.01 | -14.25 | -6.64 | -0.11 |
Bud Site | -5.23 | 1.26 | -15.14 | -10.05 | -14.61 | -2.03 | -1.61 | -4.03 | -3.68 | -3.80 | -4.89 | -2.99 | -14.82 | -11.62 | -12.79 |
Cell Periphery | 0.12 | -13.53 | -7.83 | -7.74 | 12.57 | 0.73 | -7.54 | -3.55 | -3.68 | 0.40 | 0.62 | -12.00 | -8.51 | -8.63 | 5.77 |
Cytoplasm | 18.61 | 31.60 | 44.20 | 25.05 | -9.34 | 9.03 | 18.93 | 28.35 | 24.52 | 18.86 | 19.20 | 29.58 | 44.25 | 29.88 | 11.00 |
Cytoplasmic Foci | -4.75 | -5.52 | -23.11 | -18.02 | -9.88 | -6.72 | -7.91 | -8.65 | -7.83 | -7.47 | -7.90 | -8.50 | -25.75 | -21.14 | -18.19 |
Eisosomes | -4.71 | -7.77 | -3.32 | -1.67 | 4.38 | -3.10 | -3.82 | -1.87 | -1.70 | -1.81 | -4.52 | -8.25 | -3.54 | -1.68 | -1.61 |
Endoplasmic Reticulum | -5.52 | 3.31 | 4.25 | 7.83 | 0.55 | 2.73 | 4.68 | 4.50 | 3.33 | 0.23 | 1.32 | 4.68 | 5.55 | 8.30 | 2.98 |
Endosome | -5.09 | 0.43 | -7.43 | -5.75 | -7.49 | -1.54 | -1.88 | -4.03 | -3.62 | -3.42 | -4.28 | -4.26 | -6.65 | -5.44 | -4.69 |
Golgi | 5.81 | -3.47 | 3.40 | -3.45 | 6.52 | 0.85 | 0.37 | -1.07 | -1.27 | -1.16 | 4.76 | 1.53 | 1.82 | -2.87 | 0.40 |
Lipid Particles | -2.21 | -8.54 | -17.52 | -16.59 | -4.42 | -1.47 | -7.57 | -4.91 | -4.81 | -4.14 | -2.59 | -10.13 | -18.27 | -17.53 | -12.41 |
Mitochondria | -7.07 | -1.25 | -1.96 | 0.33 | 0.64 | -2.00 | -1.21 | 0.12 | 1.97 | 0.97 | -2.61 | -2.24 | -1.27 | 1.71 | 0.59 |
Mitotic Spindle | -5.92 | -0.21 | -18.57 | -16.22 | -18.56 | -5.13 | -7.12 | -3.38 | -2.71 | -2.04 | -7.56 | -8.09 | -18.32 | -15.73 | -11.80 |
None | -3.78 | 4.55 | 0.46 | 3.25 | -2.20 | -1.82 | 1.18 | 8.80 | 7.08 | 5.39 | -3.38 | 0.32 | 2.39 | 4.63 | 1.63 |
Nuclear Periphery | -11.46 | -8.64 | -15.84 | -1.74 | 8.05 | -1.52 | -7.95 | -4.73 | -4.45 | -1.90 | -7.40 | -12.09 | -9.93 | -3.55 | 9.04 |
Nuclear Periphery Foci | -6.16 | -5.02 | -3.93 | 4.01 | 2.56 | -1.01 | 0.25 | -0.82 | -0.18 | -0.98 | -4.77 | -3.09 | 0.06 | 5.23 | 3.43 |
Nucleolus | -3.43 | -3.02 | -5.84 | 1.71 | 0.07 | -1.59 | -2.92 | -1.56 | -0.60 | -1.22 | -2.63 | -5.00 | -3.25 | 1.72 | 1.64 |
Nucleus | -22.32 | -9.45 | -6.53 | 19.76 | 8.21 | -6.58 | -0.84 | 4.19 | 7.56 | 4.56 | -19.31 | -9.53 | 9.91 | 27.20 | 16.20 |
Peroxisomes | -2.31 | -0.78 | -4.29 | -3.29 | -3.18 | -3.19 | -1.72 | -4.03 | -1.73 | -2.64 | -3.93 | -1.91 | -4.38 | -3.25 | -3.62 |
Vacuole | -6.84 | -17.14 | -19.99 | -16.66 | 12.97 | -1.42 | -19.38 | -7.07 | -6.97 | -3.10 | -5.21 | -24.34 | -19.61 | -17.74 | 9.01 |
Vacuole Periphery | 4.46 | -6.35 | 3.17 | -2.65 | 7.43 | 2.13 | -2.16 | -1.78 | -2.88 | -0.72 | 4.89 | -3.36 | 2.41 | -3.32 | 6.16 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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