Standard name | |
---|---|
Human Ortholog | |
Description | Oligomeric mitochondrial matrix chaperone; cooperates with Ssc1p in mitochondrial thermotolerance after heat shock; able to prevent the aggregation of misfolded proteins as well as resolubilize protein aggregates |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 5 | 0 | 1 | 3 | 0 | 2 | 3 | 0 | – | 4 | 1 | 0 | 0 | 1 | 1 | 2 | 1 | 7 | 13 | 11 | 9 | 19 | 10 |
Bud | 4 | 4 | 7 | 6 | 11 | 3 | 5 | 5 | 8 | – | 19 | 6 | 1 | 0 | 1 | 0 | 0 | 0 | 5 | 8 | 7 | 10 | 9 | 4 |
Bud Neck | 0 | 5 | 1 | 5 | 1 | 3 | 2 | 0 | 0 | – | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 13 | 13 | 11 | 10 | 14 | 8 |
Bud Site | 4 | 3 | 1 | 1 | 0 | 2 | 6 | 1 | 2 | – | 1 | 9 | 1 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 2 | 0 | 3 | 2 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 1 | 2 | 5 | 4 | 6 | 9 | 14 | 13 | – | 16 | 6 | 1 | 5 | 3 | 1 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 4 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 0 |
Endosome | 3 | 7 | 16 | 7 | 7 | 5 | 6 | 4 | 0 | – | 2 | 2 | 3 | 7 | 8 | 0 | 3 | 2 | 8 | 19 | 17 | 4 | 22 | 10 |
Golgi | 0 | 2 | 3 | 0 | 3 | 1 | 1 | 5 | 0 | – | 2 | 2 | 0 | 1 | 0 | 6 | 19 | 2 | 6 | 16 | 15 | 6 | 13 | 5 |
Mitochondria | 65 | 114 | 81 | 96 | 116 | 150 | 204 | 212 | 174 | – | 264 | 173 | 64 | 37 | 47 | 13 | 16 | 6 | 50 | 96 | 73 | 41 | 28 | 15 |
Nucleus | 2 | 4 | 0 | 1 | 0 | 3 | 3 | 3 | 3 | – | 3 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 3 | 4 | 0 | 1 | 0 | 3 | 4 | 2 | 1 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 53 | 75 | 32 | 26 | 26 | 47 | 82 | 107 | 56 | – | 91 | 93 | 19 | 39 | 54 | 37 | 52 | 19 | 17 | 48 | 14 | 8 | 15 | 8 |
Peroxisomes | 20 | 37 | 37 | 21 | 41 | 18 | 34 | 17 | 5 | – | 21 | 19 | 45 | 88 | 90 | 1 | 8 | 1 | 25 | 29 | 30 | 25 | 109 | 68 |
SpindlePole | 18 | 16 | 38 | 11 | 19 | 8 | 15 | 14 | 6 | – | 12 | 5 | 19 | 46 | 53 | 3 | 8 | 4 | 40 | 36 | 38 | 18 | 57 | 47 |
Vac/Vac Membrane | 4 | 4 | 10 | 10 | 9 | 12 | 7 | 22 | 5 | – | 2 | 8 | 2 | 3 | 2 | 4 | 9 | 5 | 3 | 12 | 4 | 3 | 2 | 1 |
Unique Cell Count | 137 | 220 | 170 | 152 | 186 | 219 | 310 | 338 | 232 | 350 | 259 | 122 | 162 | 188 | 60 | 100 | 38 | 188 | 312 | 230 | 149 | 309 | 185 | |
Labelled Cell Count | 179 | 283 | 228 | 194 | 242 | 261 | 380 | 409 | 273 | 438 | 326 | 158 | 227 | 262 | 70 | 120 | 45 | 188 | 312 | 230 | 149 | 309 | 185 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 20.3 | 19.1 | 18.1 | 15.5 | 18.2 | 18.4 | 19.1 | 22.6 | 20.1 | – | 22.2 | 24.8 | 20.2 | 24.5 | 22.7 | 49.0 | 43.3 | 38.4 | 16.9 | 18.3 | 18.8 |
Std Deviation (1e-4) | 11.4 | 11.1 | 10.1 | 9.0 | 8.2 | 8.5 | 8.3 | 11.5 | 10.0 | – | 12.2 | 12.5 | 9.7 | 13.8 | 10.3 | 30.0 | 23.1 | 18.8 | 8.1 | 9.7 | 9.4 |
Intensity Change (Log2) | – | – | – | -0.22 | 0.01 | 0.02 | 0.07 | 0.32 | 0.15 | – | 0.3 | 0.45 | 0.16 | 0.44 | 0.32 | 1.44 | 1.26 | 1.08 | -0.1 | 0.01 | 0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | -0.1 | 0.8 | 0 | 0 | 0 | -0.3 | – | 0.6 | -1.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 1.8 | 2.3 | – | 2.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -1.7 | -2.2 | -3.1 | -3.7 | -4.5 | -4.8 | – | -5.2 | -4.4 | -2.4 | -1.8 | -1.9 | 0 | -2.0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 5.0 | 0 |
Mitochondria | 2.8 | 2.8 | 4.2 | 3.9 | 3.2 | 5.6 | – | 6.3 | 3.9 | 0.8 | -4.7 | -4.5 | -3.5 | -5.2 | -3.6 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -0.4 | -1.2 | 0.6 | 1.9 | 3.1 | 1.3 | – | 1.8 | 3.8 | -0.7 | 1.2 | 2.2 | 6.2 | 5.7 | 4.0 |
Peroxisomes | -1.9 | 0.1 | -3.8 | -3.2 | -5.8 | -6.3 | – | -5.4 | -4.3 | 2.8 | 6.1 | 5.2 | -3.6 | -2.9 | -2.8 |
SpindlePole | -3.8 | -3.1 | -5.7 | -5.9 | -6.4 | -6.3 | – | -6.9 | -6.9 | -1.4 | 1.3 | 1.3 | -3.0 | -3.0 | -1.6 |
Vacuole | 0.3 | -0.4 | -0.2 | -2.1 | 0.3 | -1.9 | – | 0 | -1.4 | -1.8 | -1.9 | -2.5 | 0 | 1.0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 14.7075 | 17.8777 | 16.2619 | 14.5445 | 15.9877 | 17.4341 | 15.1657 | 18.1814 | 15.6584 | 15.8544 | 12.9313 | 15.9648 | 13.5806 | 16.7448 | 14.2533 | 14.5094 | 14.0091 | 14.7998 |
Actin | 0.0068 | 0.0005 | 0.0124 | 0.0021 | 0.0138 | 0.0074 | 0.008 | 0.0005 | 0.0102 | 0.0004 | 0.0008 | 0.0021 | 0.0182 | 0.0078 | 0.0049 | 0.0218 | 0.0007 | 0.0012 |
Bud | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0003 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0003 |
Bud Neck | 0.0021 | 0.0001 | 0.0003 | 0 | 0.0002 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0019 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 |
Bud Periphery | 0.0003 | 0.0004 | 0.0005 | 0.0001 | 0.0004 | 0 | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0004 | 0.0002 | 0.0007 | 0.0002 | 0.0003 | 0.0002 |
Bud Site | 0.002 | 0.0002 | 0.0035 | 0.0007 | 0.0003 | 0.0001 | 0.0031 | 0 | 0.0003 | 0 | 0.0002 | 0 | 0.0007 | 0.001 | 0.0053 | 0.0003 | 0.0002 | 0.0007 |
Cell Periphery | 0.0004 | 0.0005 | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0.0002 | 0.0001 |
Cytoplasm | 0.0008 | 0 | 0.002 | 0.0012 | 0.0006 | 0.0001 | 0.001 | 0 | 0.0001 | 0 | 0.0013 | 0.0001 | 0.0022 | 0 | 0.0002 | 0 | 0 | 0.0003 |
Cytoplasmic Foci | 0.0145 | 0.0029 | 0.0121 | 0.0178 | 0.0062 | 0.0039 | 0.0048 | 0.0022 | 0.0019 | 0 | 0.0176 | 0.0016 | 0.019 | 0.0028 | 0.0119 | 0.0031 | 0.003 | 0.0033 |
Eisosomes | 0.0005 | 0.0001 | 0.0006 | 0.0002 | 0.0001 | 0.0002 | 0.0006 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0025 | 0 | 0.0005 | 0.0014 | 0.0013 | 0 | 0.0034 | 0 | 0.0004 | 0 | 0.0004 | 0 | 0.0023 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 |
Endosome | 0.0037 | 0.0014 | 0.0061 | 0.0025 | 0.031 | 0.0006 | 0.0053 | 0.0032 | 0.0042 | 0.0001 | 0.0213 | 0.0016 | 0.0095 | 0.0036 | 0.0027 | 0.0059 | 0.0138 | 0.0108 |
Golgi | 0.0049 | 0.0021 | 0.0109 | 0.0013 | 0.008 | 0.002 | 0.003 | 0.0119 | 0.0037 | 0.0003 | 0.0395 | 0.0113 | 0.0097 | 0.0251 | 0.0063 | 0.0071 | 0.0247 | 0.0288 |
Lipid Particles | 0.0546 | 0.023 | 0.0205 | 0.034 | 0.011 | 0.0099 | 0.0152 | 0.0115 | 0.0094 | 0.0011 | 0.0246 | 0.0117 | 0.024 | 0.0304 | 0.0102 | 0.0167 | 0.0222 | 0.0181 |
Mitochondria | 0.8634 | 0.932 | 0.8804 | 0.9178 | 0.8742 | 0.9639 | 0.9203 | 0.943 | 0.9528 | 0.9959 | 0.8689 | 0.9616 | 0.8728 | 0.8903 | 0.9271 | 0.9213 | 0.8757 | 0.9262 |
None | 0.002 | 0 | 0.002 | 0.0064 | 0.0006 | 0.0003 | 0.0011 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0011 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Nuclear Periphery | 0.0017 | 0 | 0.0012 | 0.0006 | 0.0046 | 0 | 0.0049 | 0 | 0.0009 | 0 | 0.0008 | 0.0001 | 0.001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 |
Nucleolus | 0.0028 | 0.0045 | 0.0063 | 0.0005 | 0.0008 | 0.0004 | 0.0017 | 0.0004 | 0.0002 | 0 | 0.0003 | 0.0004 | 0.0002 | 0.0011 | 0.0053 | 0.0001 | 0.001 | 0.0002 |
Nucleus | 0.0009 | 0.0002 | 0.003 | 0.0002 | 0.0007 | 0 | 0.0011 | 0 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0002 |
Peroxisomes | 0.0267 | 0.0135 | 0.0225 | 0.0059 | 0.0207 | 0.0101 | 0.0125 | 0.0168 | 0.0117 | 0.0011 | 0.0085 | 0.0046 | 0.0278 | 0.0206 | 0.0149 | 0.0199 | 0.0138 | 0.0025 |
Punctate Nuclear | 0.0016 | 0 | 0.0021 | 0.001 | 0.001 | 0 | 0.0022 | 0 | 0.0002 | 0 | 0.0028 | 0 | 0.0029 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 |
Vacuole | 0.0012 | 0.0062 | 0.003 | 0.0014 | 0.0139 | 0.0002 | 0.005 | 0.0012 | 0.0005 | 0.0001 | 0.0037 | 0.0007 | 0.0014 | 0.0026 | 0.0022 | 0.001 | 0.005 | 0.0014 |
Vacuole Periphery | 0.0064 | 0.0122 | 0.0093 | 0.0046 | 0.01 | 0.0009 | 0.0051 | 0.0089 | 0.0026 | 0.0006 | 0.008 | 0.0039 | 0.004 | 0.0132 | 0.0057 | 0.0019 | 0.0387 | 0.0048 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.3239 | 20.2458 | 26.1405 | 51.8851 | 30.8322 | 32.1422 | 59.0575 | 84.1408 | 99.7414 | 51.5825 |
Translational Efficiency | 2.107 | 2.2281 | 1.7205 | 1.8056 | 1.8655 | 1.7331 | 1.4521 | 1.6427 | 1.4739 | 1.6975 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1504 | 1315 | 2302 | 1692 | 2315 | 1607 | 96 | 88 | 3819 | 2922 | 2398 | 1780 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1041.64 | 1843.53 | 3547.73 | 2769.43 | 1961.79 | 1797.80 | 3119.26 | 3296.01 | 1599.42 | 1818.38 | 3530.58 | 2795.46 |
Standard Deviation | 407.42 | 713.51 | 923.48 | 980.68 | 738.60 | 762.54 | 1046.19 | 1057.47 | 773.42 | 741.22 | 932.49 | 991.21 |
Intensity Change Log 2 | — | 0.823614 | 1.768039 | 1.410732 | — | -0.125938 | 0.669033 | 0.748550 | — | 0.277854 | 1.150425 | 1.014001 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002098 | 0.011867 | 0.008107 | 0.019499 | 0.007824 | 0.011474 | 0.023515 | 0.026169 | 0.005569 | 0.011651 | 0.008723 | 0.019828 |
Bud Neck | 0.025876 | 0.015702 | 0.003169 | 0.010889 | 0.009705 | 0.018742 | 0.017348 | 0.015072 | 0.016074 | 0.017374 | 0.003737 | 0.011096 |
Bud Site | 0.056615 | 0.026464 | 0.002776 | 0.027018 | 0.019412 | 0.038139 | 0.014064 | 0.035141 | 0.034063 | 0.032885 | 0.003228 | 0.027420 |
Cell Periphery | 0.001014 | 0.000355 | 0.000093 | 0.000517 | 0.000283 | 0.000564 | 0.000337 | 0.000488 | 0.000571 | 0.000470 | 0.000103 | 0.000515 |
Cytoplasm | 0.019654 | 0.006027 | 0.000600 | 0.004535 | 0.005374 | 0.009031 | 0.001258 | 0.007059 | 0.010998 | 0.007679 | 0.000627 | 0.004660 |
Cytoplasmic Foci | 0.530259* | 0.307922* | 0.038121 | 0.082766 | 0.198506* | 0.284142* | 0.178796 | 0.148970 | 0.329157* | 0.294844* | 0.043753 | 0.086039 |
Eisosomes | 0.001477 | 0.002316 | 0.001632 | 0.001011 | 0.003029 | 0.003616 | 0.002013 | 0.002853 | 0.002418 | 0.003031 | 0.001648 | 0.001102 |
Endoplasmic Reticulum | 0.000703 | 0.000444 | 0.000078 | 0.000333 | 0.000242 | 0.000411 | 0.000509 | 0.000317 | 0.000424 | 0.000426 | 0.000095 | 0.000332 |
Endosome | 0.023541 | 0.005812 | 0.000858 | 0.005855 | 0.002467 | 0.006416 | 0.004980 | 0.004451 | 0.010766 | 0.006144 | 0.001023 | 0.005786 |
Golgi | 0.013881 | 0.020299 | 0.011804 | 0.026122 | 0.011879 | 0.020809 | 0.025375 | 0.023235 | 0.012667 | 0.020579 | 0.012348 | 0.025980 |
Lipid Particles | 0.009683 | 0.024742 | 0.012306 | 0.010480 | 0.019131 | 0.020088 | 0.009245 | 0.004853 | 0.015410 | 0.022182 | 0.012183 | 0.010202 |
Mitochondria | 0.052897 | 0.264248* | 0.854913* | 0.678049* | 0.487830* | 0.280690* | 0.501168* | 0.589742* | 0.316545* | 0.273291* | 0.840752* | 0.673683* |
Mitotic Spindle | 0.014994 | 0.027650 | 0.007942 | 0.021689 | 0.010722 | 0.018541 | 0.093092 | 0.018231 | 0.012405 | 0.022640 | 0.011351 | 0.021518 |
None | 0.004628 | 0.001338 | 0.002463 | 0.001873 | 0.002073 | 0.002168 | 0.002202 | 0.001787 | 0.003079 | 0.001794 | 0.002452 | 0.001868 |
Nuclear Periphery | 0.000240 | 0.000521 | 0.001271 | 0.000888 | 0.000558 | 0.000543 | 0.001943 | 0.000897 | 0.000433 | 0.000533 | 0.001297 | 0.000889 |
Nuclear Periphery Foci | 0.005399 | 0.001189 | 0.000432 | 0.000602 | 0.000936 | 0.001116 | 0.000853 | 0.000482 | 0.002694 | 0.001149 | 0.000449 | 0.000596 |
Nucleolus | 0.018085 | 0.010352 | 0.002458 | 0.002617 | 0.007706 | 0.010042 | 0.003783 | 0.001464 | 0.011793 | 0.010181 | 0.002511 | 0.002560 |
Nucleus | 0.011526 | 0.002808 | 0.000128 | 0.000512 | 0.002020 | 0.003262 | 0.000364 | 0.000288 | 0.005763 | 0.003057 | 0.000137 | 0.000501 |
Peroxisomes | 0.185608* | 0.253399* | 0.040479 | 0.079686 | 0.201604* | 0.253943* | 0.084614 | 0.102666* | 0.195305* | 0.253698* | 0.042246 | 0.080822 |
Vacuole | 0.017895 | 0.011532 | 0.004558 | 0.011222 | 0.005868 | 0.011095 | 0.019506 | 0.006868 | 0.010604 | 0.011292 | 0.005156 | 0.011007 |
Vacuole Periphery | 0.003927 | 0.005012 | 0.005811 | 0.013837 | 0.002830 | 0.005169 | 0.015037 | 0.008970 | 0.003262 | 0.005098 | 0.006181 | 0.013596 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -8.21 | -6.62 | -10.95 | -4.04 | -6.53 | -2.89 | -1.75 | -2.31 | -1.85 | -0.22 | -6.82 | -3.25 | -9.04 | -4.83 | -6.37 |
Bud Neck | 5.71 | 14.34 | 8.87 | 4.54 | -11.69 | -7.98 | -2.68 | -1.84 | 1.17 | 0.55 | -1.40 | 16.34 | 5.30 | 6.82 | -11.21 |
Bud Site | 8.54 | 17.81 | 8.11 | -0.34 | -12.34 | -7.07 | 1.79 | -1.27 | 0.14 | -1.64 | 0.43 | 20.26 | 2.45 | 2.03 | -12.25 |
Cell Periphery | 5.85 | 8.23 | 3.69 | -1.94 | -5.14 | -3.55 | -0.37 | -1.64 | 0.48 | -0.91 | 1.55 | 8.62 | 0.56 | -0.56 | -5.24 |
Cytoplasm | 9.05 | 13.23 | 10.09 | 2.78 | -9.97 | -4.92 | 10.38 | -0.59 | 0.77 | -2.13 | 4.40 | 16.65 | 8.49 | 5.32 | -10.14 |
Cytoplasmic Foci | 18.08 | 55.14 | 46.48 | 24.14 | -10.50 | -9.43 | 0.79 | 1.99 | 5.35 | 0.88 | 4.26 | 48.68 | 35.73 | 30.82 | -9.70 |
Eisosomes | -3.21 | -0.82 | 2.46 | 6.54 | 5.91 | -1.68 | 2.82 | 0.18 | 0.75 | -0.81 | -2.70 | 4.80 | 8.61 | 9.86 | 4.86 |
Endoplasmic Reticulum | 2.03 | 8.42 | 4.69 | 1.69 | -8.27 | -5.11 | -1.03 | -0.99 | 1.26 | 0.72 | -0.35 | 9.62 | 2.21 | 2.25 | -7.58 |
Endosome | 8.30 | 10.99 | 8.28 | -0.14 | -9.92 | -5.33 | -2.70 | -2.39 | 1.81 | 0.44 | 4.79 | 11.59 | 5.06 | 0.46 | -9.87 |
Golgi | -3.02 | 1.37 | -5.74 | -2.38 | -7.31 | -5.28 | -2.72 | -2.00 | -0.42 | 0.29 | -5.98 | 0.26 | -7.28 | -2.66 | -7.19 |
Lipid Particles | -5.97 | -1.86 | -0.57 | 5.74 | 1.33 | -0.44 | 5.09 | 8.00 | 7.47 | 2.40 | -4.08 | 2.59 | 3.75 | 7.05 | 1.51 |
Mitochondria | -19.98* | -123.31* | -64.19* | -31.91* | 18.01* | 16.83* | -0.33 | -2.53 | -7.71 | -1.60 | 4.81 | -63.80* | -33.34* | -37.25* | 17.10* |
Mitotic Spindle | -4.01 | 3.58 | -2.55 | 1.83 | -6.61 | -4.22 | -3.96 | -1.47 | 0.06 | 3.50 | -5.95 | 0.75 | -4.51 | 0.40 | -4.70 |
None | 3.83 | 2.56 | 3.24 | -3.68 | 4.93 | -0.27 | -0.29 | 1.14 | 0.97 | 0.92 | 3.31 | 1.83 | 3.48 | -0.31 | 5.04 |
Nuclear Periphery | -5.93 | -4.63 | -6.42 | -3.48 | 1.57 | 0.07 | -3.07 | -0.86 | -1.00 | 1.95 | -0.81 | -3.58 | -3.08 | -3.40 | 1.75 |
Nuclear Periphery Foci | 5.03 | 5.98 | 5.79 | 4.03 | -1.51 | -1.08 | 0.46 | 1.89 | 2.82 | 1.55 | 4.44 | 6.45 | 6.10 | 5.25 | -1.37 |
Nucleolus | 4.18 | 10.58 | 10.36 | 6.28 | -0.42 | -2.08 | 4.08 | 8.43 | 9.32 | 3.11 | 1.64 | 12.28 | 11.82 | 9.69 | -0.16 |
Nucleus | 7.03 | 10.53 | 10.05 | 4.70 | -4.37 | -1.95 | 4.20 | 4.38 | 5.89 | 0.71 | 4.23 | 11.40 | 10.31 | 6.96 | -4.35 |
Peroxisomes | -6.89 | 22.67 | 14.61 | 19.70 | -8.59 | -5.93 | 7.81 | 5.28 | 7.83 | -0.78 | -8.86 | 33.34 | 20.36 | 26.66 | -8.61 |
Vacuole | 3.01 | 7.50 | 3.30 | 0.18 | -6.68 | -5.43 | -2.34 | -0.72 | 2.50 | 2.10 | -0.75 | 6.35 | -0.48 | 0.19 | -5.98 |
Vacuole Periphery | -1.27 | -2.63 | -7.58 | -6.46 | -6.20 | -2.99 | -2.34 | -2.49 | -1.49 | 1.05 | -3.23 | -5.11 | -8.84 | -6.81 | -5.91 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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