Standard name
Human Ortholog
Description Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.09
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0.05 0 0.06 0.06 0 0 0 0.05 0 0.08 0 0 0 0 0 0
Cytoplasm 0.99 0.94 1.0 0.97 0.92 0.91 0.82 0.76 0.74 0.72 0.83 0.77 0.98 0.97 0.98 0.97 0.92 0.96 0.91 0.89 0.9 0.93 0.91 0.78
Endoplasmic Reticulum 0 0 0 0 0 0 0.07 0.09 0 0.06 0.06 0.05 0 0 0 0.06 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.12 0.13 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.07 0.1 0.09 0.1 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 1 2 0 5 1 4 0 1 0 0 0 0 0 0 0 0 1 2
Bud 1 0 0 0 0 1 1 1 1 4 0 1 2 1 4 0 0 0 8 4 0 1 9 13
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 1 1 4 3 4 5 4 2 3 6 2 6 10 6 5 8 0 0 0 0 0 0
Cytoplasm 244 63 83 88 85 104 132 112 61 89 45 82 171 303 315 109 112 99 226 81 77 110 205 114
Endoplasmic Reticulum 0 1 0 1 1 1 11 13 3 8 3 5 1 0 1 7 4 6 1 0 3 0 2 3
Endosome 3 0 0 0 0 1 1 1 0 0 0 1 1 2 2 0 1 0 1 0 0 0 0 1
Golgi 0 0 0 0 0 0 1 2 0 2 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Mitochondria 2 1 2 2 3 4 6 2 10 16 2 11 1 2 0 2 3 2 0 0 0 0 0 2
Nucleus 0 1 0 0 0 0 0 1 0 1 0 2 0 0 3 0 1 0 0 0 0 0 0 0
Nuclear Periphery 0 1 0 2 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 1 0 1 0 1 1 0 0 0 0 0 0 1 0 0 1 0
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 1 1 0 0 0 0 0 2
SpindlePole 1 0 0 0 0 0 0 0 0 0 0 1 1 3 2 1 0 1 1 0 0 0 0 0
Vac/Vac Membrane 1 2 0 1 4 2 12 15 7 12 4 2 0 5 3 2 5 1 1 0 0 1 2 1
Unique Cell Count 247 67 83 91 92 114 161 148 82 124 54 106 175 313 323 112 122 103 250 92 86 118 227 148
Labelled Cell Count 255 70 86 95 97 117 170 155 87 141 58 116 181 325 340 127 133 118 250 92 86 118 227 148


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.3 21.9 18.9 15.1 22.4 25.9 45.1 45.4 50.4 52.1 60.4 53.8 12.5 23.6 44.9 13.8 18.2 21.2 16.3 13.4 13.7
Std Deviation (1e-4) 8.1 15.3 11.5 10.1 20.0 19.5 30.7 30.3 39.1 42.3 44.0 40.2 12.7 23.3 33.6 15.1 31.7 25.4 37.1 10.7 12.4
Intensity Change (Log2) – – – -0.33 0.24 0.45 1.25 1.26 1.41 1.46 1.67 1.51 -0.59 0.32 1.24 -0.46 -0.06 0.16 -0.22 -0.5 -0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.7 -2.6 -2.8 -4.1 -4.9 -4.9 -5.3 -3.8 -4.6 -1.4 -1.6 -1.4 -1.5 -2.7 -1.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 2.4 2.6 0 2.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 35.6444 47.0392 40.9041 45.4819 46.5966 49.6432 27.744 33.8023 35.3307 23.8904 16.7745 30.0711 30.6636 43.778 40.9372 50.4078 39.8668 44.7291
Actin 0.0038 0.0026 0.004 0.0002 0.0328 0.0041 0.0134 0.0024 0.0029 0.0056 0.0071 0.0004 0.0272 0.0028 0.0163 0.0485 0.0382 0.0026
Bud 0.0026 0.0115 0.0023 0.0004 0.0008 0.0015 0.0011 0.0039 0.0022 0.0286 0.0126 0.0006 0.0064 0.0112 0.0053 0.0014 0.0006 0.0011
Bud Neck 0.001 0.0004 0.0006 0.0006 0.0009 0.0025 0.0004 0.0002 0.0002 0.0003 0.0004 0.0003 0.0006 0.0003 0.0009 0.0005 0.0013 0.0022
Bud Periphery 0.0011 0.0054 0.0009 0.0001 0.0007 0.0012 0.0007 0.0006 0.0011 0.0291 0.0114 0.0001 0.0037 0.0033 0.0031 0.0007 0.0003 0.0005
Bud Site 0.002 0.0101 0.0013 0.0002 0.0013 0.0004 0.0018 0.0065 0.0006 0.0054 0.0008 0 0.0042 0.006 0.0041 0.0011 0.0007 0.0002
Cell Periphery 0.0012 0.0012 0.0004 0.0001 0.0002 0.001 0.0003 0.0004 0.0001 0.0003 0.0002 0 0.0009 0.0004 0.0013 0.0014 0.001 0.0002
Cytoplasm 0.8655 0.954 0.9081 0.9346 0.9258 0.9621 0.8984 0.9665 0.968 0.8625 0.8818 0.989 0.8134 0.9662 0.9151 0.8716 0.885 0.9277
Cytoplasmic Foci 0.0251 0.0019 0.0103 0.007 0.0017 0.0019 0.0121 0.0043 0.0047 0.0184 0.005 0.0021 0.02 0.0027 0.0068 0.0238 0.0088 0.0043
Eisosomes 0.0001 0 0.0001 0 0.0002 0 0.0001 0 0 0 0 0 0.0008 0 0.0002 0.0005 0.0004 0
Endoplasmic Reticulum 0.0111 0.0012 0.0067 0.0009 0.0056 0.0173 0.0158 0.0018 0.0016 0.0011 0.0044 0.003 0.013 0.0006 0.0029 0.0017 0.0033 0.0026
Endosome 0.0062 0.0007 0.0054 0.0017 0.0074 0.0018 0.0247 0.0044 0.0025 0.0221 0.0187 0.001 0.0152 0.0003 0.0045 0.002 0.0069 0.0095
Golgi 0.0034 0.0001 0.0021 0.001 0.0028 0.0002 0.0084 0.004 0.0002 0.0066 0.0029 0.0001 0.0047 0.0001 0.0043 0.0022 0.0039 0.0207
Lipid Particles 0.007 0.0001 0.0024 0.0154 0.002 0.0001 0.0038 0.0002 0 0.0054 0.0001 0 0.0086 0 0.002 0.0052 0.0045 0.0013
Mitochondria 0.0059 0.0003 0.0212 0.0012 0.001 0.0001 0.0017 0.001 0.0002 0.0016 0.0375 0.0001 0.0033 0.0003 0.0071 0.0064 0.0056 0.0007
None 0.01 0.0009 0.0054 0.0004 0.0008 0.0004 0.0075 0.0017 0.0011 0.0018 0.0017 0.0015 0.0239 0.0022 0.0042 0.0128 0.0152 0.0021
Nuclear Periphery 0.0087 0.0014 0.0062 0.0012 0.0048 0.0014 0.0029 0.0004 0.0046 0.0012 0.0032 0.0003 0.0132 0.0008 0.0018 0.0029 0.0038 0.0049
Nucleolus 0.0032 0.0001 0.0014 0.0002 0.0001 0 0.0003 0.0001 0.0001 0.0001 0.0001 0 0.0014 0 0.0008 0.0034 0.0027 0.0003
Nucleus 0.0079 0.0017 0.0039 0.0015 0.0018 0.002 0.0018 0.0008 0.007 0.0012 0.0035 0.0007 0.011 0.0013 0.0029 0.0022 0.0032 0.0049
Peroxisomes 0.0036 0.0001 0.0015 0.028 0.0003 0 0.001 0 0.0001 0.0029 0.0004 0 0.0101 0.0001 0.0007 0.0015 0.0013 0.0002
Punctate Nuclear 0.0109 0.0001 0.0024 0.0002 0.0009 0.0001 0.0009 0.0002 0.0003 0.0007 0.0002 0.0001 0.0067 0.0003 0.001 0.0011 0.0018 0.0008
Vacuole 0.0158 0.0049 0.0097 0.0044 0.0063 0.0017 0.0023 0.0006 0.0021 0.004 0.0044 0.0005 0.0092 0.0011 0.0115 0.0076 0.008 0.0081
Vacuole Periphery 0.0039 0.0014 0.0037 0.0008 0.0017 0.0004 0.0006 0.0001 0.0005 0.0011 0.0039 0.0001 0.0024 0.0001 0.0034 0.0016 0.0035 0.0052

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 109.735 37.209 53.7153 170.9298 74.0818 330.5355 109.1037 135.9642 281.4788 140.7014
Translational Efficiency 1.7184 1.3958 1.3504 1.8136 1.6242 1.6904 1.6523 1.5951 1.6985 1.7277

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2024 1419 1711 1080 1825 1424 204 131 3849 2843 1915 1211

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1173.57 1267.18 20397.56 3505.18 717.73 980.88 5528.30 3245.94 957.43 1123.78 18813.58 3477.14
Standard Deviation 574.53 647.78 7881.27 2512.53 228.18 524.30 4203.34 1683.14 500.07 606.32 8855.70 2437.80
Intensity Change Log 2 — 0.110718 4.119421 1.578585 — 0.450635 2.945323 2.177123 — 0.249302 3.776942 1.835749

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000130 0.000180 0.000090 0.000415 0.000125 0.000290 0.000533 0.000386 0.000127 0.000235 0.000137 0.000412
Bud Neck 0.001682 0.002276 0.001000 0.003009 0.014305 0.006355 0.007604 0.003650 0.007667 0.004319 0.001703 0.003079
Bud Site 0.030194 0.022028 0.002647 0.019013 0.010498 0.033393 0.011551 0.023846 0.020855 0.027721 0.003595 0.019536
Cell Periphery 0.001043 0.000478 0.019498 0.011289 0.000233 0.000339 0.032096 0.011689 0.000659 0.000408 0.020840 0.011332
Cytoplasm 0.854338* 0.852190* 0.814174* 0.762128* 0.565313* 0.708390* 0.482519* 0.672107* 0.717297* 0.780163* 0.778843* 0.752390*
Cytoplasmic Foci 0.062679 0.054511 0.009780 0.016389 0.208536* 0.139808 0.043970 0.023929 0.131837 0.097234 0.013422 0.017205
Eisosomes 0.000011 0.000005 0.000002 0.000006 0.000148 0.000035 0.000013 0.000007 0.000076 0.000020 0.000004 0.000006
Endoplasmic Reticulum 0.001045 0.000453 0.000155 0.000168 0.001077 0.000582 0.000248 0.000076 0.001060 0.000517 0.000165 0.000158
Endosome 0.002522 0.004388 0.000165 0.002512 0.013945 0.018233 0.002101 0.001198 0.007938 0.011323 0.000371 0.002370
Golgi 0.005578 0.003014 0.011405 0.007347 0.002588 0.004662 0.011800 0.003991 0.004160 0.003840 0.011447 0.006984
Lipid Particles 0.000553 0.000966 0.005843 0.016587 0.006870 0.001490 0.031409 0.020797 0.003548 0.001229 0.008566 0.017042
Mitochondria 0.000382 0.000162 0.000255 0.000562 0.002425 0.001246 0.000232 0.000044 0.001351 0.000705 0.000253 0.000506
Mitotic Spindle 0.001318 0.003242 0.000521 0.004081 0.000325 0.006678 0.004835 0.000839 0.000848 0.004963 0.000981 0.003731
None 0.008894 0.006723 0.004167 0.002449 0.007030 0.002688 0.005207 0.000796 0.008010 0.004702 0.004277 0.002270
Nuclear Periphery 0.000035 0.000058 0.001110 0.000176 0.000315 0.000092 0.001971 0.000178 0.000168 0.000075 0.001201 0.000177
Nuclear Periphery Foci 0.000620 0.001218 0.004979 0.003330 0.000410 0.000712 0.013233 0.004248 0.000520 0.000964 0.005858 0.003430
Nucleolus 0.000150 0.000208 0.000085 0.000164 0.000760 0.000481 0.000156 0.000154 0.000439 0.000345 0.000092 0.000163
Nucleus 0.009590 0.016374 0.050361 0.025149 0.107836 0.012387 0.089347 0.018606 0.056173 0.014377 0.054514 0.024441
Peroxisomes 0.000278 0.000698 0.000011 0.000227 0.003528 0.003547 0.000095 0.000291 0.001819 0.002125 0.000020 0.000234
Vacuole 0.012120 0.028417 0.055346 0.121741 0.052359 0.055978 0.254130* 0.209910* 0.031199 0.042222 0.076522 0.131279
Vacuole Periphery 0.006838 0.002410 0.018408 0.003256 0.001372 0.002614 0.006949 0.003258 0.004246 0.002512 0.017187 0.003256

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.14 0.75 -6.40 -5.05 -6.54 -6.20 -4.72 -3.86 -1.86 1.06 -6.87 -2.40 -7.17 -4.43 -5.90
Bud Neck -1.83 2.13 -7.18 -3.99 -8.61 7.71 3.96 8.91 2.61 4.69 6.15 11.53 6.45 0.48 -6.34
Bud Site 3.47 16.63 2.99 -0.39 -11.26 -9.71 -1.08 -3.05 1.71 -2.32 -4.12 16.28 -1.30 2.08 -11.41
Cell Periphery 2.90 -15.43 -10.05 -10.86 7.22 -1.40 -6.53 -4.01 -3.96 3.53 2.16 -17.08 -11.32 -11.66 8.00
Cytoplasm 0.71 7.76 13.22 11.97 6.10 -14.84 4.61 -2.95 2.42 -5.37 -9.52 -5.60 -0.71 6.03 3.68
Cytoplasmic Foci 1.94 17.71 13.17 9.88 -4.69 10.51 21.40 25.70 15.80 2.25 8.57 37.06 33.11 22.79 -3.31
Eisosomes 3.66 5.41 1.92 -3.02 -6.55 15.77 19.36 20.71 8.75 3.24 15.05 20.87 19.72 8.18 -4.96
Endoplasmic Reticulum 2.78 4.69 4.70 2.76 0.24 2.24 4.44 5.78 3.58 3.27 3.53 6.85 6.99 4.07 0.80
Endosome -3.57 10.18 1.02 3.65 -4.03 -2.55 10.92 12.30 12.84 2.35 -3.86 15.11 9.40 11.59 -3.73
Golgi 4.40 -8.59 -0.04 -2.06 4.42 -3.95 -5.82 -1.23 1.42 4.58 0.91 -12.52 -1.12 -1.41 4.97
Lipid Particles -2.77 -11.17 -11.80 -11.38 -6.71 10.30 -6.78 -3.70 -5.27 2.28 8.49 -8.36 -10.33 -12.26 -5.27
Mitochondria 4.92 1.89 -0.61 -1.78 -1.08 2.96 8.06 9.83 3.74 2.08 3.26 8.78 4.43 1.09 -0.92
Mitotic Spindle -2.53 4.73 -3.19 -1.16 -3.90 -4.66 -2.62 -1.09 0.89 0.42 -5.26 -1.00 -3.74 0.23 -3.46
None 1.52 5.07 6.49 3.28 3.86 3.76 1.99 6.05 3.55 2.53 3.53 5.43 7.47 3.24 4.45
Nuclear Periphery -2.53 -8.38 -11.10 -9.05 6.98 4.33 -5.75 1.19 -2.57 6.01 3.61 -8.60 -2.36 -7.58 8.18
Nuclear Periphery Foci -3.85 -11.61 -8.77 -6.13 3.67 -3.38 -7.99 -6.61 -5.99 5.36 -4.95 -14.05 -10.33 -8.33 5.40
Nucleolus -1.17 2.01 -0.64 0.80 -3.58 1.43 7.99 8.29 1.90 0.33 0.84 11.07 7.42 2.05 -3.26
Nucleus -5.24 -19.86 -13.28 -7.83 7.27 28.28 1.38 12.12 -1.59 7.21 21.11 -1.78 8.90 -9.39 9.41
Peroxisomes -4.67 15.15 2.15 5.30 -7.42 0.05 11.76 10.17 6.51 -2.34 -1.02 12.82 11.16 7.78 -7.67
Vacuole -8.07 -14.87 -20.06 -16.53 -10.58 -1.29 -10.84 -7.43 -7.23 1.29 -5.97 -13.09 -18.54 -16.26 -8.52
Vacuole Periphery 4.36 -7.36 4.32 -0.49 11.74 -2.38 -4.64 -1.51 -0.13 2.29 2.98 -10.16 2.72 -0.41 11.89
Standard name
Human Ortholog
Description Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (72%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Hxk1

Hxk1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hxk1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available