Standard name
Human Ortholog
Description Thiol peroxidase; functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor; HYR1 has a paralog, GPX1, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0.06 0 0 0 0.06 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.99 0.98 0.98 0.97 0.88 – 0.66 0.72 0.76 0.69 0.71 0.99 1.0 0.98 0.96 0.96 0.94 0.93 0.94 0.94 0.82 0.9 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.08 0 0.1 – 0.15 0.21 0.17 0.26 0.24 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.05 – 0.23 0.12 0.12 0.06 0.09 0 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 0 0 0 0 – 0 0 0 3 1 0 0 0 0 0 0 4 2 0 1 0 0
Bud 4 0 4 0 0 0 – 2 2 1 1 0 0 0 0 0 0 1 11 4 0 2 4 3
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 3 1 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 12 5 0 2 3 5 – 6 9 6 7 13 0 1 2 25 4 12 0 0 0 0 0 1
Cytoplasm 985 570 169 172 185 169 – 125 205 197 160 156 211 163 200 423 325 373 949 539 162 89 135 178
Endoplasmic Reticulum 4 2 0 0 1 2 – 2 4 5 8 4 0 0 1 9 8 6 6 3 0 2 1 2
Endosome 0 0 0 0 0 0 – 0 1 0 0 0 0 0 1 0 0 0 8 3 0 0 1 1
Golgi 1 1 0 0 0 0 – 0 0 0 0 0 0 0 0 1 1 0 5 3 0 3 0 0
Mitochondria 12 2 7 14 1 19 – 29 60 45 60 52 2 0 0 2 3 0 7 2 1 0 1 0
Nucleus 3 0 0 1 0 1 – 3 4 8 7 2 0 0 0 1 2 1 0 1 0 1 0 0
Nuclear Periphery 2 0 0 0 0 1 – 0 6 2 0 0 0 0 0 0 0 0 2 1 0 1 0 0
Nucleolus 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 4 1 0 0 0 0
Vac/Vac Membrane 13 4 0 0 3 10 – 44 34 30 15 20 1 0 1 18 10 21 4 3 1 3 1 1
Unique Cell Count 1015 577 172 176 190 192 190 284 260 233 220 213 163 204 442 338 395 1020 573 173 110 150 193
Labelled Cell Count 1037 585 180 189 193 207 211 326 294 261 248 214 164 205 479 353 414 1020 573 173 110 150 193


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.3 11.4 6.4 7.7 10.4 9.4 – 9.3 9.5 10.0 9.5 10.2 10.5 12.7 12.3 35.1 33.1 33.1 12.0 11.9 13.8
Std Deviation (1e-4) 1.9 2.9 1.6 1.9 2.4 2.2 – 2.6 3.3 3.3 3.2 4.0 2.5 3.4 3.4 11.0 9.9 10.2 4.5 3.4 5.4
Intensity Change (Log2) – – – 0.28 0.71 0.55 – 0.55 0.57 0.64 0.57 0.68 0.72 0.99 0.95 2.46 2.37 2.37 0.91 0.89 1.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 3.2 0 0 0 3.2 0 0
Cytoplasm -0.4 -0.6 -3.8 – -7.9 -7.0 -6.4 -7.5 -7.1 0.7 1.7 -0.2 -1.5 -1.3 -2.0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.5 0 2.2 – 3.6 5.0 4.1 5.8 5.4 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 6.7 4.7 4.6 3.4 4.1 0 0 0 2.7 0 3.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.6233 5.7743 5.4886 5.1776 4.7405 5.2542 5.0329 5.5091 4.3362 4.0104 3.0651 4.3693 6.8767 7.181 6.8396 6.701 6.7716 6.861
Actin 0.0062 0.0003 0.0022 0.0003 0.0151 0.0006 0.0123 0.0148 0.014 0.0257 0.0051 0.0071 0.0004 0.0025 0.0005 0.0008 0.0024 0.0013
Bud 0.0001 0.0003 0.0008 0.0002 0.0005 0.0002 0.0046 0.0163 0.0089 0.0022 0.0039 0.003 0.0001 0.0003 0.0044 0.0002 0.0112 0.0003
Bud Neck 0.0003 0.0003 0.0008 0.0004 0.0017 0.0008 0.0045 0.0006 0.0011 0.0005 0.0024 0.0014 0.0002 0.0002 0.0003 0.0003 0.0021 0.0015
Bud Periphery 0.0001 0.0001 0.0006 0 0.0003 0.0001 0.0027 0.0059 0.0059 0.0027 0.0094 0.0027 0 0.0001 0.001 0.0001 0.0145 0.0001
Bud Site 0.0005 0.0026 0.0011 0.0001 0.0007 0.0001 0.0043 0.0258 0.0154 0.004 0.0024 0.0003 0.0001 0.0006 0.0009 0.0002 0.0012 0.0001
Cell Periphery 0 0 0 0 0 0 0.0001 0.0009 0.0005 0.0002 0.0004 0.0001 0 0.0001 0.0001 0 0.0001 0
Cytoplasm 0.9364 0.9711 0.942 0.976 0.9385 0.9588 0.8157 0.875 0.8419 0.7948 0.7923 0.9146 0.9461 0.9528 0.9307 0.9406 0.9332 0.945
Cytoplasmic Foci 0.0067 0.0035 0.0073 0.0038 0.008 0.0074 0.0446 0.008 0.0184 0.0131 0.0413 0.0166 0.0063 0.009 0.0026 0.0055 0.006 0.0059
Eisosomes 0.0001 0 0 0 0 0 0 0.0001 0.0002 0.0002 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0028 0.0018 0.002 0.0011 0.0023 0.0024 0.0022 0.0016 0.0031 0.0033 0.0033 0.002 0.0021 0.0013 0.0021 0.0022 0.0013 0.0027
Endosome 0.0051 0.0021 0.0056 0.0019 0.0051 0.0053 0.0198 0.007 0.0104 0.0102 0.0232 0.0048 0.0026 0.001 0.0011 0.0024 0.0013 0.0021
Golgi 0.0006 0.0001 0.0005 0.0001 0.0015 0.0004 0.0074 0.0009 0.0065 0.0063 0.0156 0.002 0.0009 0.0003 0.0001 0.0002 0.0003 0.0002
Lipid Particles 0.0005 0 0.0003 0 0.0004 0.0001 0.0074 0.0003 0.0061 0.0092 0.0308 0.007 0.0078 0.0001 0 0.0001 0 0
Mitochondria 0.0006 0.0001 0.0002 0.0001 0.0008 0.0006 0.003 0.001 0.0074 0.0032 0.0183 0.0049 0.0004 0.0001 0.0002 0.0001 0.0061 0.0002
None 0.029 0.0094 0.0268 0.008 0.0089 0.0143 0.0279 0.0319 0.0304 0.1035 0.0068 0.0221 0.0194 0.0246 0.049 0.0404 0.0098 0.0319
Nuclear Periphery 0.0024 0.001 0.0013 0.0009 0.0016 0.0014 0.003 0.0014 0.0069 0.0031 0.0038 0.0009 0.0008 0.0007 0.001 0.0011 0.0008 0.0014
Nucleolus 0.0001 0 0.0001 0 0.0001 0 0.0002 0.0002 0.001 0.0005 0.0005 0.0001 0 0 0.0001 0 0.0002 0
Nucleus 0.0046 0.0053 0.0044 0.0056 0.0065 0.0052 0.0157 0.0034 0.0052 0.0049 0.0037 0.0034 0.0023 0.0031 0.0046 0.0043 0.0069 0.0054
Peroxisomes 0.0008 0.0001 0.0005 0 0.0036 0.0004 0.018 0.0025 0.0101 0.0065 0.0261 0.0044 0.0096 0.0018 0.0001 0.0001 0.0006 0.0003
Punctate Nuclear 0.0024 0.0005 0.0021 0.0005 0.0035 0.0008 0.004 0.0013 0.0034 0.0044 0.0046 0.0016 0.0005 0.0009 0.0005 0.0009 0.0017 0.001
Vacuole 0.0007 0.0011 0.0011 0.0007 0.0007 0.0008 0.0018 0.0009 0.0024 0.0012 0.0044 0.0008 0.0004 0.0004 0.0006 0.0005 0.0004 0.0005
Vacuole Periphery 0.0002 0.0001 0.0002 0.0002 0.0002 0.0002 0.0006 0.0002 0.0009 0.0004 0.0014 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 169.3232 136.3284 124.9643 118.8768 132.3025 181.5575 132.267 111.5037 143.5461 122.5712
Translational Efficiency 2.3466 2.2032 1.7992 2.4204 2.1128 2.4119 1.9699 2.2679 1.8135 2.0155

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1816 1577 649 1292 492 1377 1823 1382 2308 2954 2472 2674

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 904.28 951.89 1614.95 1692.69 841.49 981.52 1494.78 1612.63 890.89 965.70 1526.33 1651.31
Standard Deviation 233.88 160.75 342.16 470.72 150.50 155.70 302.44 423.40 220.29 159.11 317.78 448.68
Intensity Change Log 2 — 0.074025 0.836648 0.904476 — 0.222072 0.828915 0.938397 — 0.147284 0.832926 0.920926

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000131 0.000258 0.000490 0.003833 0.000268 0.000422 0.000305 0.003555 0.000160 0.000335 0.000353 0.003689
Bud Neck 0.001502 0.003596 0.001905 0.004339 0.003014 0.003339 0.001805 0.002934 0.001824 0.003476 0.001832 0.003613
Bud Site 0.003738 0.011032 0.007357 0.034775 0.006811 0.011406 0.009644 0.029634 0.004393 0.011207 0.009043 0.032118
Cell Periphery 0.000101 0.000074 0.000275 0.000360 0.000187 0.000103 0.000078 0.000145 0.000119 0.000088 0.000130 0.000249
Cytoplasm 0.782778* 0.757298* 0.716692* 0.689658* 0.611787* 0.718823* 0.826394* 0.751951* 0.746327* 0.739363* 0.797593* 0.721853*
Cytoplasmic Foci 0.084247 0.108523 0.014100 0.028231 0.175020 0.116192 0.009741 0.022909 0.103597 0.112098 0.010885 0.025480
Eisosomes 0.000022 0.000024 0.000006 0.000023 0.000087 0.000034 0.000003 0.000011 0.000036 0.000029 0.000004 0.000017
Endoplasmic Reticulum 0.001157 0.000755 0.000513 0.001092 0.001704 0.000552 0.000119 0.000994 0.001274 0.000660 0.000222 0.001042
Endosome 0.005038 0.008134 0.000898 0.020820 0.016381 0.012172 0.001052 0.013209 0.007456 0.010016 0.001012 0.016887
Golgi 0.001139 0.001973 0.000412 0.027984 0.001795 0.003872 0.000295 0.015940 0.001279 0.002858 0.000325 0.021760
Lipid Particles 0.001034 0.001100 0.001164 0.002850 0.004728 0.001141 0.000530 0.001294 0.001822 0.001119 0.000696 0.002046
Mitochondria 0.001095 0.000969 0.000179 0.011290 0.006970 0.002397 0.000122 0.006825 0.002347 0.001635 0.000137 0.008983
Mitotic Spindle 0.000928 0.007201 0.005483 0.050410 0.000531 0.007556 0.014926 0.060251 0.000843 0.007366 0.012447 0.055496
None 0.006569 0.002616 0.008951 0.003703 0.001932 0.001373 0.007375 0.002978 0.005581 0.002036 0.007789 0.003328
Nuclear Periphery 0.000111 0.000123 0.000412 0.000463 0.000362 0.000127 0.000184 0.000542 0.000165 0.000125 0.000244 0.000504
Nuclear Periphery Foci 0.002416 0.001548 0.004541 0.003942 0.003840 0.001750 0.001861 0.003518 0.002720 0.001642 0.002565 0.003722
Nucleolus 0.000505 0.000304 0.000116 0.000211 0.000853 0.000428 0.000106 0.000244 0.000579 0.000362 0.000109 0.000228
Nucleus 0.043901 0.028993 0.197731* 0.042417 0.050247 0.025803 0.096088 0.027458 0.045254 0.027506 0.122773 0.034685
Peroxisomes 0.000537 0.002216 0.000072 0.002098 0.001543 0.001947 0.001264 0.002070 0.000751 0.002090 0.000951 0.002084
Vacuole 0.062308 0.062183 0.038592 0.058181 0.109771 0.089520 0.028052 0.046347 0.072425 0.074926 0.030819 0.052065
Vacuole Periphery 0.000743 0.001079 0.000110 0.013320 0.002170 0.001042 0.000055 0.007189 0.001047 0.001062 0.000069 0.010151

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.78 -8.88 -3.29 -2.93 -1.46 -1.08 -0.27 -2.14 -1.63 -2.10 -2.55 -6.30 -3.99 -3.23 -2.97
Bud Neck -4.32 -8.07 -6.65 1.08 2.37 -0.52 2.72 1.55 2.36 -2.58 -4.78 -2.47 -4.50 2.41 -2.01
Bud Site -7.97 -3.66 -9.47 -3.67 -4.97 -3.42 0.29 -5.22 -1.77 -5.25 -9.06 -3.87 -11.58 -3.81 -7.69
Cell Periphery 2.73 -9.00 -7.09 -8.36 2.44 3.35 4.53 2.70 -1.69 -2.41 2.61 -5.72 -6.19 -7.71 -1.62
Cytoplasm 3.71 15.83 11.59 7.81 -4.69 -7.79 -10.72 -9.87 -2.92 0.24 1.54 5.06 4.84 3.47 0.17
Cytoplasmic Foci -5.25 20.84 21.22 23.40 -0.14 7.32 22.54 21.62 25.83 -5.46 -2.14 31.33 29.28 34.72 -5.48
Eisosomes -1.11 7.54 5.55 6.38 -0.21 9.50 15.35 15.17 12.63 -1.40 3.78 17.70 15.40 13.81 -2.13
Endoplasmic Reticulum 1.33 1.70 2.92 3.82 4.35 3.90 5.25 4.79 2.90 -0.96 2.53 4.10 4.14 4.76 0.54
Endosome -4.25 10.69 0.61 3.36 -3.67 2.90 12.66 9.75 7.22 -4.12 -3.27 16.74 5.80 7.68 -5.63
Golgi -1.96 2.39 -3.36 -2.76 -3.89 -2.12 3.99 -1.87 0.18 -3.99 -3.15 4.59 -4.09 -2.03 -5.56
Lipid Particles -0.38 -6.96 -7.06 -6.86 -3.14 4.95 5.13 3.96 -3.03 -4.04 3.52 1.75 -3.93 -7.48 -6.06
Mitochondria 0.23 1.50 -1.13 -1.47 -2.38 1.53 2.33 1.82 0.92 -2.69 0.87 2.82 0.47 -0.50 -3.48
Mitotic Spindle -3.91 -4.55 -6.85 -3.73 -4.37 -4.23 -7.43 -7.44 -4.00 -3.13 -5.55 -8.01 -9.98 -5.48 -4.60
None 5.13 0.29 5.33 0.29 6.73 0.82 -5.19 -0.57 -4.67 10.39 5.80 -0.78 5.14 -1.99 12.28
Nuclear Periphery -0.84 -16.89 -9.01 -7.36 8.41 2.55 -0.32 0.43 -3.23 1.38 1.30 -13.56 -5.23 -6.37 4.55
Nuclear Periphery Foci 1.93 -5.89 -1.42 -5.32 5.59 2.83 1.93 1.96 -1.94 0.23 2.70 -1.93 -0.11 -5.10 2.93
Nucleolus 0.99 1.74 1.76 5.15 0.35 1.58 3.51 3.39 2.33 -1.45 1.16 2.75 2.63 3.55 -1.57
Nucleus 9.42 -26.77 -4.33 -11.27 24.44 5.69 -14.06 2.52 -7.16 26.91 11.74 -33.26 -0.65 -13.07 33.22
Peroxisomes -3.71 4.58 -0.07 3.47 -2.43 -0.92 3.10 2.60 4.53 -1.13 -4.67 2.71 1.11 5.22 -1.67
Vacuole -0.88 -6.01 -17.97 -17.41 -9.07 2.60 2.28 -3.75 -8.04 -7.57 -1.85 -6.38 -18.97 -18.30 -12.53
Vacuole Periphery -1.12 3.09 -2.47 -2.07 -3.35 1.72 3.33 0.73 -1.16 -3.37 0.04 4.72 -2.22 -2.36 -4.68
Standard name
Human Ortholog
Description Thiol peroxidase; functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor; HYR1 has a paralog, GPX1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Hyr1

Hyr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hyr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available