Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit; required for respiration and for maintenance of the mitochondrial genome
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0.11 0.11 0.07 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0.05 0.07 0 0.1 – 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Golgi 0 0 0 0.07 0.19 0 0.05 – 0 0 0 0 0.07 0.06 0.17 0.43 0.47 0.21 0 0.15 0.06 0.06 0.07 0.06
Mitochondria 0.9 1.0 1.0 0.88 0.68 0.88 0.7 – 0.89 0.87 0.93 0.93 0.88 0.91 0.88 0.07 0.06 0.1 0.84 0.7 0.85 0.75 0.73 0.71
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.07 0 0 0 0.06 0.06 0.12 – 0.08 0.05 0.1 0.12 0.07 0.05 0.05 0.07 0.06 0.2 0 0 0 0 0 0
Nucleolus 0.18 0 0 0 0 0.08 0.09 – 0.07 0.08 0.05 0.05 0 0 0 0 0.06 0.13 0 0 0 0 0 0
Peroxisomes 0 0 0 0.06 0 0 0 – 0 0 0 0 0 0 0.05 0.12 0.09 0.1 0.06 0 0.05 0.09 0.09 0.09
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Vac/Vac Membrane 0 0.08 0.05 0.11 0.2 0.09 0.09 – 0 0 0.05 0 0.09 0.08 0.08 0.14 0.22 0.2 0 0.07 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 – 0 0 1 2 4 0 0 5 4 1 1 1 0 2 9 16
Bud 2 1 0 1 2 7 3 – 14 5 10 1 2 5 2 4 6 6 0 0 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 0 0 4 0 – 3 5 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 5 5 2 3 – 3 2 1 3 4 12 14 15 18 4 0 0 0 0 1 0
Cytoplasm 0 0 0 1 7 6 12 – 7 3 4 3 4 5 5 4 4 1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 4 3 1 – 2 3 6 6 4 2 3 2 1 1 0 0 0 0 0 1
Endosome 1 0 0 10 27 3 55 – 2 8 1 2 4 16 11 10 1 1 0 0 0 5 10 18
Golgi 0 0 0 14 70 2 29 – 3 4 3 9 19 30 76 57 75 13 2 9 3 8 29 26
Mitochondria 60 36 20 190 255 260 392 – 323 300 349 272 258 428 396 9 10 6 69 45 48 120 316 313
Nucleus 0 0 0 0 1 7 13 – 6 4 1 2 0 1 1 0 0 0 0 0 0 0 1 0
Nuclear Periphery 5 0 0 8 22 19 68 – 30 18 39 34 19 25 23 9 10 12 1 0 0 3 5 6
Nucleolus 12 0 0 8 14 24 53 – 27 29 19 14 9 12 5 1 9 8 0 0 0 0 1 3
Peroxisomes 1 0 0 13 8 0 7 – 5 13 3 1 6 10 23 16 15 6 4 0 2 13 37 38
SpindlePole 0 0 0 1 6 4 19 – 8 6 7 3 2 4 3 2 1 4 0 0 0 0 3 3
Vac/Vac Membrane 2 3 1 24 74 28 52 – 14 15 19 11 26 37 35 19 35 12 1 4 0 1 10 5
Unique Cell Count 67 36 20 215 374 296 558 362 346 375 294 292 469 449 134 158 61 83 65 57 160 435 441
Labelled Cell Count 83 40 22 275 496 369 707 447 415 463 364 361 587 597 153 189 75 83 65 57 160 435 441


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.0 8.7 7.3 8.4 9.4 6.1 8.0 – 6.9 7.2 6.7 6.8 9.9 8.7 10.1 10.2 10.2 10.2 9.3 9.5 9.8
Std Deviation (1e-4) 1.7 1.5 1.0 1.4 1.9 1.6 1.7 – 1.6 1.5 1.7 1.5 2.0 1.7 1.8 2.5 2.2 2.3 2.0 2.2 2.1
Intensity Change (Log2) – – – 0.19 0.36 -0.27 0.12 – -0.1 -0.02 -0.13 -0.11 0.43 0.24 0.46 0.48 0.48 0.47 0.34 0.36 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 3.7 4.1 0
Mitochondria -1.6 -3.0 -1.7 -2.9 – -1.5 -1.7 -1.2 -1.3 -1.6 -1.4 -1.6 0 0 -7.5
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.7324 5.0464 5.65 5.1006 5.0054 5.5866 7.2182 6.8859 6.9199 6.4693 6.4808 6.9707 9.5939 8.6325 9.3911 8.9744 8.5824 9.2207
Actin 0.0018 0.0012 0.0005 0.0036 0.0012 0.0014 0.0091 0.004 0.0021 0.0005 0.0008 0.0002 0.0055 0.0021 0.0037 0.002 0.0001 0.0008
Bud 0.0001 0 0.0001 0.0004 0.0001 0 0.0001 0.0002 0.0001 0.0003 0.001 0 0.0001 0.0004 0.0001 0.0001 0 0
Bud Neck 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0 0.0002 0.0001 0 0 0 0
Bud Periphery 0.0003 0.0001 0.0001 0.001 0.0003 0.0001 0.0003 0.0005 0.0004 0.0017 0.0009 0 0.0002 0.0006 0.0002 0.0002 0 0.0001
Bud Site 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0003 0.0003 0 0.0001 0.0006 0 0.0004 0.001 0.0002 0.0001 0 0
Cell Periphery 0.0003 0 0 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0002 0.0001 0 0.0001 0.0002 0.0001 0 0 0.0001
Cytoplasm 0 0 0.0001 0 0 0.0005 0.0008 0 0 0 0.0008 0 0.0005 0.0001 0 0 0 0
Cytoplasmic Foci 0.0018 0.0013 0.0015 0.0006 0.0006 0.0004 0.0046 0.0013 0.0005 0.0002 0.0032 0.0001 0.0039 0.0034 0.0007 0.0009 0.0017 0.0004
Eisosomes 0.0003 0.0001 0.0001 0.0002 0.0002 0.0001 0.0003 0.0002 0.0004 0.0001 0.0001 0.0001 0.0007 0.0001 0.0005 0.0001 0 0.0001
Endoplasmic Reticulum 0.0001 0 0 0 0 0.0012 0.0003 0 0.0001 0.0001 0.0001 0 0.0008 0 0.0002 0 0 0
Endosome 0.0071 0.0011 0.0063 0.0017 0.0027 0.0043 0.0076 0.0064 0.0052 0.0019 0.016 0.0025 0.0074 0.0054 0.0057 0.0053 0.0328 0.0035
Golgi 0.0115 0.0036 0.0124 0.0042 0.0071 0.0065 0.0092 0.0233 0.0063 0.001 0.013 0.0053 0.018 0.0169 0.0076 0.0304 0.0315 0.0164
Lipid Particles 0.0121 0.0007 0.0014 0.051 0.0043 0.0008 0.012 0.0038 0.0019 0.0007 0.0079 0.0007 0.02 0.0138 0.0059 0.0003 0.0039 0.0067
Mitochondria 0.9427 0.9755 0.9716 0.9291 0.9791 0.9804 0.9345 0.9537 0.972 0.9761 0.9472 0.9885 0.9193 0.9369 0.9612 0.9558 0.9138 0.9605
None 0.0001 0 0 0.0001 0 0.0003 0.0007 0 0 0 0.0001 0 0.0021 0 0 0 0 0
Nuclear Periphery 0.0002 0 0 0.0004 0 0.0008 0.0016 0 0.0002 0.0001 0.0001 0 0.0018 0 0.0001 0 0.0001 0.0002
Nucleolus 0.0001 0 0 0.0002 0 0 0.0008 0.0001 0.0001 0.0002 0.0001 0 0.0003 0.0001 0.0001 0 0.0001 0
Nucleus 0.0001 0 0 0 0 0.0002 0.0006 0 0.0001 0.0001 0.0001 0 0.0004 0.0001 0 0 0 0
Peroxisomes 0.0145 0.0149 0.0038 0.0008 0.003 0.0013 0.006 0.004 0.0036 0.0013 0.0025 0.0006 0.0124 0.0081 0.0051 0.0037 0.0026 0.0049
Punctate Nuclear 0 0 0 0 0 0.0001 0.005 0 0 0 0.0001 0 0.0015 0 0 0 0 0
Vacuole 0.0015 0.0002 0.0004 0.0007 0.0002 0.0004 0.001 0.0005 0.003 0.0072 0.0032 0.0002 0.001 0.0021 0.0009 0.0002 0.0056 0.0007
Vacuole Periphery 0.0053 0.0013 0.0014 0.0057 0.0008 0.0012 0.0049 0.0016 0.0039 0.0082 0.0022 0.0016 0.0034 0.0087 0.0076 0.0009 0.0075 0.0055

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 56.252 40.6155 60.2769 39.7496 57.505 69.1949 62.5772 62.0332 70.6178 63.0334
Translational Efficiency 1.0776 1.1885 0.754 1.7774 1.1048 1.2658 1.1457 1.4999 1.2477 1.3082

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1612 932 89 91 131 1857 256 1037 1743 2789 345 1128

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 818.75 977.00 1440.72 1205.22 967.65 1010.73 1263.78 1129.37 829.94 999.46 1309.43 1135.49
Standard Deviation 156.37 179.10 224.32 172.50 149.16 165.99 182.33 189.25 160.71 171.23 208.91 189.08
Intensity Change Log 2 — 0.254936 0.815295 0.557802 — 0.062840 0.385188 0.222961 — 0.154067 0.598307 0.386114

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002409 0.006857 0.005128 0.010103 0.001372 0.003600 0.005015 0.011313 0.002331 0.004689 0.005044 0.011215
Bud Neck 0.003813 0.002045 0.005895 0.001951 0.003134 0.000561 0.009333 0.001313 0.003762 0.001057 0.008446 0.001364
Bud Site 0.008201 0.007411 0.007234 0.009089 0.009031 0.001351 0.014110 0.006833 0.008263 0.003376 0.012336 0.007015
Cell Periphery 0.001148 0.000301 0.000181 0.000898 0.000463 0.000260 0.000370 0.000521 0.001096 0.000274 0.000321 0.000551
Cytoplasm 0.001882 0.001090 0.001166 0.001052 0.000810 0.000290 0.001173 0.000821 0.001801 0.000557 0.001171 0.000840
Cytoplasmic Foci 0.007879 0.022576 0.083334 0.039418 0.036943 0.012614 0.076760 0.010050 0.010063 0.015943 0.078456 0.012419
Eisosomes 0.000089 0.000134 0.000242 0.000158 0.000069 0.000100 0.000153 0.000188 0.000087 0.000112 0.000176 0.000185
Endoplasmic Reticulum 0.000347 0.000221 0.000925 0.001337 0.000332 0.000108 0.000559 0.000450 0.000345 0.000145 0.000653 0.000522
Endosome 0.016541 0.024594 0.036113 0.042518 0.032025 0.012409 0.043509 0.011495 0.017705 0.016481 0.041601 0.013997
Golgi 0.011956 0.038473 0.110572* 0.105123 0.042251 0.023805 0.058495 0.038368 0.014233 0.028707 0.071929 0.043753
Lipid Particles 0.004399 0.007937 0.015142 0.001035 0.017329 0.005216 0.008412 0.000894 0.005371 0.006125 0.010148 0.000905
Mitochondria 0.907091* 0.841155* 0.623039* 0.722475* 0.791001* 0.913735* 0.655341* 0.891933* 0.898366* 0.889481* 0.647008* 0.878262*
Mitotic Spindle 0.001419 0.002239 0.032870 0.012684 0.006031 0.000942 0.027873 0.003033 0.001766 0.001376 0.029162 0.003812
None 0.000940 0.000389 0.001740 0.000507 0.000382 0.000292 0.000541 0.000485 0.000898 0.000325 0.000850 0.000487
Nuclear Periphery 0.000065 0.000144 0.008414 0.000452 0.000347 0.000083 0.001351 0.000078 0.000086 0.000103 0.003173 0.000108
Nuclear Periphery Foci 0.000289 0.000230 0.005323 0.000460 0.000580 0.000112 0.001056 0.000073 0.000311 0.000152 0.002157 0.000104
Nucleolus 0.000655 0.000143 0.000504 0.000068 0.000210 0.000074 0.000637 0.000062 0.000622 0.000097 0.000603 0.000062
Nucleus 0.000460 0.000098 0.000432 0.000077 0.000128 0.000033 0.000427 0.000029 0.000435 0.000055 0.000428 0.000033
Peroxisomes 0.023217 0.036126 0.013122 0.009180 0.037398 0.020341 0.068505 0.014656 0.024282 0.025616 0.054218 0.014214
Vacuole 0.003376 0.004076 0.016825 0.020552 0.008077 0.002133 0.015172 0.002355 0.003729 0.002782 0.015599 0.003823
Vacuole Periphery 0.003825 0.003761 0.031798 0.020862 0.012090 0.001941 0.011208 0.005052 0.004446 0.002549 0.016519 0.006327

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.12 -1.88 -1.81 -0.74 -1.13 -3.48 -3.92 -5.02 -3.80 -2.95 -2.96 -2.95 -4.65 -3.41 -3.14
Bud Neck 2.14 -1.38 1.85 0.13 2.46 1.86 -3.12 1.29 -2.36 5.40 3.74 -3.54 3.11 -0.99 6.02
Bud Site 0.33 -0.27 -0.30 -0.50 0.10 1.60 -0.88 0.43 -3.52 1.91 3.24 -1.47 0.64 -2.33 1.83
Cell Periphery 3.65 4.33 0.56 -1.50 -1.82 0.90 0.41 -0.26 -1.86 -1.05 3.77 3.51 2.40 -2.34 -1.86
Cytoplasm 1.14 0.27 1.26 0.09 0.60 2.04 -1.23 -0.04 -4.80 1.84 2.14 0.84 1.67 -1.85 1.63
Cytoplasmic Foci -6.91 -6.35 -3.52 -1.84 2.95 3.42 -3.85 3.77 1.54 8.65 -4.28 -10.54 -1.41 2.09 10.07
Eisosomes -3.43 -2.63 -1.82 -0.62 1.23 -2.95 -4.19 -6.91 -5.42 -1.49 -2.33 -4.02 -5.87 -4.94 -0.41
Endoplasmic Reticulum 2.17 -3.62 -2.49 -2.82 -0.96 3.06 -1.65 -0.92 -3.25 0.70 4.11 -2.90 -1.57 -3.67 0.95
Endosome -3.11 -2.42 -2.66 -1.81 -0.50 3.39 -1.53 3.51 0.57 6.35 0.74 -5.46 1.91 1.45 6.28
Golgi -7.86 -5.93 -4.81 -3.40 0.19 2.32 -1.63 0.46 -3.73 2.81 -7.31 -8.85 -7.74 -3.82 3.81
Lipid Particles -2.19 -2.09 4.64 4.63 2.77 1.87 1.33 2.55 4.93 3.96 -0.69 -2.29 5.46 6.74 4.79
Mitochondria 6.28 7.78 5.17 3.26 -2.04 -4.52 3.82 -3.65 2.57 -9.61 1.26 12.24 2.23 1.34 -10.99
Mitotic Spindle -1.29 -3.30 -2.46 -2.28 1.94 2.07 -3.28 1.12 -2.01 3.95 0.77 -5.24 -1.83 -2.36 4.78
None 1.96 -2.07 1.53 -2.15 4.76 1.50 -2.39 -1.60 -4.01 1.00 2.21 0.21 1.58 -3.94 4.27
Nuclear Periphery -3.40 -2.31 -2.60 -2.06 2.20 1.66 -2.98 1.69 0.24 4.26 -0.85 -3.20 -0.96 -0.23 3.17
Nuclear Periphery Foci 0.58 -2.26 -0.95 -1.50 2.18 1.74 -1.53 1.89 1.42 5.87 1.69 -3.08 2.18 1.73 3.47
Nucleolus 1.78 0.33 2.04 1.77 1.86 1.28 -2.08 1.36 0.31 3.15 1.98 0.02 2.10 1.06 3.64
Nucleus 2.16 -0.25 2.27 0.61 1.97 2.63 -1.85 2.70 0.40 2.50 2.46 -0.11 2.61 1.91 3.18
Peroxisomes -2.91 2.56 4.07 5.90 0.97 1.47 -2.08 1.96 1.91 5.40 -0.45 -3.87 3.30 4.18 5.23
Vacuole -1.03 -4.07 -2.25 -2.16 -0.30 2.63 -2.30 2.51 -0.48 5.85 1.84 -6.42 -0.12 -1.37 6.11
Vacuole Periphery 0.09 -4.82 -3.43 -3.44 1.42 3.25 0.25 2.18 -3.60 3.56 3.38 -6.06 -1.85 -4.18 4.82
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit; required for respiration and for maintenance of the mitochondrial genome
Localization
Cell Percentages mitochondrion (99%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Img1

Img1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Img1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available