Standard name
Human Ortholog
Description F1F0 ATP synthase peripheral stalk assembly factor; subunit of the matrix-exposed inner mitochondrial membrane localized INA complex (Ina22p-Ina17p) involved in assembly of the F1F0 peripheral stalk; co-purifies with Aim43p, ATP synthase subunits, and cytochrome bc1 complex assembly factors; interacts with Arh1p, a mitochondrial oxidoreductase; deletion mutant has a respiratory growth defect

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.06 0 0 0 0.06 0 0 0 0 0.16 0.12 0 0 0 0 0 0
Bud Neck 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0 0 0 0 0 0
Cytoplasm 0.23 0.31 0.53 0.44 0.31 0.17 0.15 0.12 0.08 0.1 0.1 0.53 0.49 0.5 0.16 0 0.05 0.07 0.05 0.07 0.05 0.05
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.11 0 0.07 0.07 0.13 0 0.16 0 0 0.05 0 0.18 0.24 0.2 0.21 0.12 0.12 0.08 0.08 0.09 0.08 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.26 0.12 0 0 0 0 0 0
Mitochondria 0.56 0.6 0.08 0.34 0.27 0.82 0.58 0.84 0.79 0.83 0.83 0.26 0.14 0.2 0.16 0.18 0.65 0.64 0.68 0.68 0.61 0.59
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.08 0 0 0 0 0 0 0 0.07 0.17 0.15 0.05 0.06 0 0 0 0 0 0
SpindlePole 0.09 0 0.19 0.1 0.17 0 0.16 0.05 0.1 0.07 0.07 0 0 0 0 0.18 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0.11 0.1 0.05 0.15 0.06 0.1 0.08 0.08 0.08 0.05 0.06 0.14 0.1 0.26 0.18 0 0.07 0 0 0.06 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 3 2 2 1 1 0 0 2 0 1 1 0 0 1 0 3 5 8 5 15 14
Bud 3 2 2 9 5 23 11 23 28 32 31 2 2 0 3 2 1 3 3 0 8 11
Bud Neck 0 1 14 1 5 1 1 0 2 0 2 0 0 0 0 0 1 2 4 1 6 7
Bud Site 0 2 0 1 0 8 3 8 5 6 9 0 0 0 0 0
Cell Periphery 0 2 6 9 2 1 1 7 4 9 10 0 9 9 0 2 0 0 2 1 1 2
Cytoplasm 59 80 129 135 89 72 79 75 57 57 69 121 193 168 3 0 16 22 23 14 20 17
Endoplasmic Reticulum 3 2 0 2 1 2 0 4 4 2 1 3 0 1 0 0 1 2 5 2 4 4
Endosome 27 10 18 20 36 2 87 24 28 30 24 41 93 67 4 2 44 25 33 20 31 25
Golgi 3 1 1 0 2 0 1 0 1 0 1 1 0 2 5 2 6 2 6 1 7 7
Mitochondria 143 157 20 105 77 341 312 522 542 483 604 59 55 66 3 3 234 211 291 152 254 216
Nucleus 3 2 3 1 7 6 19 9 13 11 5 0 0 1 1 0 0 0 0 0 0 1
Nuclear Periphery 0 1 0 0 0 0 2 8 3 1 3 0 0 1 0 1 4 2 2 1 2 3
Nucleolus 0 0 0 0 0 2 4 1 5 4 1 0 0 0 0 0 1 0 1 1 3 4
Peroxisomes 10 4 2 24 6 1 19 7 19 10 9 17 65 49 1 1 8 6 11 6 15 8
SpindlePole 22 4 46 32 48 17 85 33 68 41 54 7 15 4 0 3 13 11 7 3 12 8
Vac/Vac Membrane 40 30 24 14 42 23 51 51 55 47 38 13 56 35 5 3 12 24 14 8 24 22
Unique Cell Count 256 261 245 305 283 414 534 623 682 581 725 227 390 336 19 17 363 328 428 226 418 366
Labelled Cell Count 315 301 267 355 321 500 675 772 836 733 862 265 488 403 26 19 363 328 428 226 418 366


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 4.3 5.5 5.0 5.6 4.0 4.8 4.5 4.7 4.5 4.5 5.0 5.3 5.7 5.8 6.1 4.4 4.8 5.1
Std Deviation (1e-4) 0.7 0.8 1.5 1.7 1.6 1.6 1.2 1.4 2.0 1.5 1.0 1.6 1.4 2.4 0.7 1.0 1.4 2.0 2.1
Intensity Change (Log2) -0.14 0.03 -0.45 -0.21 -0.31 -0.22 -0.28 -0.3 -0.15 -0.07 0.06 0.07 0.14 -0.34 -0.19 -0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.938 -0.605 -0.7501 -0.8799 -1.0404 -0.5957 0.3879 0.6606 0.8536 0.4575 1.4259 1.1193 2.4904 3.3727 3.1451 2.7434 3.0027 3.3918
Actin 0.0236 0.0172 0.0054 0.0139 0.0095 0.0057 0.0194 0.0196 0.0346 0.0067 0.0923 0.048 0.0056 0.0112 0.0178 0.036 0.0092 0.0049
Bud 0.0013 0.0089 0.0025 0.0045 0.0039 0.0013 0.0033 0.004 0.0061 0.0405 0.0086 0.0017 0.0016 0.0031 0.0044 0.012 0.0049 0.0012
Bud Neck 0.0049 0.0019 0.0012 0.0009 0.0023 0.0078 0.0024 0.0015 0.0044 0.0021 0.0055 0.0048 0.0031 0.0014 0.0109 0.0164 0.0178 0.0099
Bud Periphery 0.0036 0.0076 0.0066 0.0241 0.0103 0.0027 0.0092 0.0086 0.0234 0.1201 0.016 0.004 0.0029 0.0062 0.0114 0.0619 0.0185 0.0029
Bud Site 0.007 0.0466 0.0089 0.0015 0.0046 0.0017 0.0178 0.0382 0.0097 0.0052 0.0202 0.0038 0.019 0.0174 0.0288 0.0037 0.0203 0.0023
Cell Periphery 0.0011 0.0016 0.001 0.0007 0.0006 0.0006 0.0035 0.0021 0.0044 0.0044 0.0019 0.0018 0.0051 0.0029 0.0031 0.0069 0.004 0.0017
Cytoplasm 0.0513 0.1166 0.0913 0.0493 0.087 0.077 0.0782 0.1114 0.1032 0.1114 0.0548 0.129 0.1355 0.1175 0.0978 0.0894 0.1285 0.1465
Cytoplasmic Foci 0.1105 0.146 0.1148 0.0985 0.1695 0.1365 0.149 0.1166 0.0883 0.0696 0.1393 0.0987 0.1299 0.1223 0.0921 0.1213 0.1002 0.115
Eisosomes 0.0008 0.0008 0.0009 0.0004 0.0003 0.0003 0.0008 0.0008 0.0019 0.0012 0.0013 0.0007 0.0007 0.0028 0.0011 0.0009 0.0011 0.0005
Endoplasmic Reticulum 0.006 0.005 0.0057 0.0039 0.0022 0.0028 0.008 0.0062 0.0052 0.0078 0.0032 0.0256 0.0097 0.0107 0.0047 0.0266 0.0037 0.0054
Endosome 0.1885 0.1509 0.1479 0.1789 0.2036 0.1585 0.1907 0.1516 0.1217 0.1502 0.1801 0.1281 0.1893 0.1618 0.0998 0.0834 0.1163 0.1379
Golgi 0.0446 0.0467 0.0334 0.0331 0.026 0.0536 0.0411 0.0477 0.0312 0.0341 0.0608 0.06 0.0404 0.0526 0.0347 0.0287 0.0427 0.0447
Lipid Particles 0.0508 0.0117 0.0282 0.0511 0.0195 0.0266 0.0779 0.0131 0.0165 0.0099 0.0423 0.0186 0.0267 0.0207 0.015 0.0287 0.0565 0.024
Mitochondria 0.2529 0.2435 0.2793 0.2935 0.2871 0.3436 0.1331 0.1551 0.1863 0.2018 0.3246 0.1806 0.1742 0.2193 0.2907 0.332 0.2952 0.2466
None 0.1695 0.1301 0.194 0.1927 0.0849 0.1056 0.1693 0.271 0.3085 0.2013 0.0143 0.198 0.2033 0.2049 0.2297 0.0658 0.1446 0.192
Nuclear Periphery 0.0093 0.002 0.0048 0.0026 0.001 0.0047 0.003 0.0015 0.0012 0.001 0.0032 0.0249 0.0022 0.0026 0.001 0.0024 0.0009 0.0013
Nucleolus 0.0022 0.0023 0.0025 0.0014 0.0011 0.0023 0.0039 0.001 0.0009 0.0006 0.0004 0.0014 0.0023 0.0011 0.0032 0.0008 0.0008 0.0007
Nucleus 0.0027 0.0043 0.0035 0.0022 0.0014 0.0035 0.0019 0.0016 0.0015 0.0013 0.0008 0.0043 0.0017 0.0018 0.0018 0.0021 0.0012 0.0016
Peroxisomes 0.0268 0.0312 0.0383 0.0214 0.0533 0.0424 0.0445 0.0276 0.0378 0.0109 0.0199 0.0414 0.0259 0.0219 0.0338 0.0408 0.0185 0.0415
Punctate Nuclear 0.0067 0.0032 0.0034 0.0028 0.0027 0.0062 0.0114 0.0022 0.0013 0.0005 0.0024 0.0023 0.0014 0.0016 0.001 0.0018 0.0008 0.0015
Vacuole 0.0251 0.0154 0.0195 0.0161 0.0198 0.0112 0.0264 0.014 0.0088 0.0134 0.0052 0.0137 0.0148 0.0115 0.0127 0.0273 0.0106 0.0127
Vacuole Periphery 0.0111 0.0065 0.0068 0.0066 0.0095 0.0053 0.0051 0.0047 0.003 0.0061 0.003 0.0088 0.0046 0.0048 0.0043 0.011 0.0038 0.0052

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.6272 6.0607 12.3675 6.3194 3.2963 11.858 7.6599 9.8229 10.2549 8.3804
Translational Efficiency 1.85 1.8996 0.6396 2.1983 3.3503 1.5199 1.3901 1.4163 1.605 2.0561

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
220 1334 1055 2044 85 2371 160 1746 305 3705 1215 3790

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 685.88 738.46 851.49 805.96 723.07 751.55 864.90 876.55 696.24 746.84 853.26 838.48
Standard Deviation 90.13 90.50 93.29 120.90 96.77 91.52 87.18 115.32 93.53 91.37 92.62 123.48
Intensity Change Log 2 0.106564 0.312033 0.232752 0.055734 0.258398 0.277701 0.080702 0.284757 0.255995

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002172 0.001664 0.001758 0.023628 0.000506 0.000564 0.005814 0.011676 0.001708 0.000960 0.002293 0.018122
Bud Neck 0.004449 0.024087 0.023185 0.030519 0.040596 0.062315 0.043988 0.034445 0.014523 0.048551 0.025925 0.032328
Bud Site 0.012862 0.029516 0.034889 0.177831* 0.013532 0.026686 0.077547 0.126293* 0.013048 0.027704 0.040507 0.154088*
Cell Periphery 0.001614 0.000407 0.000144 0.000452 0.000944 0.000287 0.000563 0.000278 0.001427 0.000331 0.000199 0.000372
Cytoplasm 0.004345 0.074764 0.289842* 0.121541* 0.145428* 0.079839 0.116578* 0.139034* 0.043664 0.078012 0.267025* 0.129600*
Cytoplasmic Foci 0.028566 0.220415* 0.085753 0.111710 0.312752* 0.197623* 0.222437* 0.134101 0.107765 0.205829* 0.103753 0.122025
Eisosomes 0.000105 0.000258 0.000086 0.000228 0.000413 0.000185 0.000292 0.000165 0.000191 0.000211 0.000114 0.000199
Endoplasmic Reticulum 0.000677 0.000930 0.007549 0.003171 0.001730 0.001031 0.005310 0.003061 0.000971 0.000994 0.007254 0.003120
Endosome 0.033277 0.070288 0.021394 0.037622 0.076181 0.086646 0.088054 0.056976 0.045234 0.080756 0.030172 0.046538
Golgi 0.074303 0.112102* 0.004228 0.055381 0.023021 0.059227 0.028198 0.042753 0.060011 0.078264 0.007384 0.049563
Lipid Particles 0.006982 0.011221 0.006140 0.002224 0.041488 0.016663 0.014734 0.004905 0.016598 0.014704 0.007272 0.003459
Mitochondria 0.763316* 0.312434* 0.013319 0.177015* 0.078969 0.293822* 0.031035 0.120498* 0.572597* 0.300523* 0.015652 0.150978*
Mitotic Spindle 0.002258 0.014793 0.045701 0.054532 0.025859 0.013633 0.014388 0.062972 0.008836 0.014051 0.041578 0.058420
None 0.000294 0.001415 0.002398 0.001063 0.001718 0.003282 0.002852 0.001306 0.000691 0.002609 0.002458 0.001175
Nuclear Periphery 0.000120 0.000203 0.004011 0.000183 0.001244 0.000414 0.000659 0.000812 0.000433 0.000338 0.003569 0.000473
Nuclear Periphery Foci 0.000275 0.000355 0.003041 0.000433 0.000975 0.000266 0.004043 0.000765 0.000470 0.000298 0.003173 0.000586
Nucleolus 0.003669 0.001327 0.003557 0.000450 0.006215 0.003720 0.003779 0.001276 0.004379 0.002858 0.003586 0.000831
Nucleus 0.015254 0.013086 0.329515* 0.013224 0.067052 0.029604 0.092227 0.030617 0.029690 0.023657 0.298267* 0.021237
Peroxisomes 0.020549 0.065406 0.015298 0.101952* 0.100462* 0.038374 0.115735* 0.063121 0.042820 0.048107 0.028524 0.084063
Vacuole 0.008785 0.031674 0.105088 0.078579 0.051399 0.071026 0.124850 0.148889 0.020661 0.056857 0.107690 0.110970
Vacuole Periphery 0.016127 0.013655 0.003102 0.008264 0.009515 0.014794 0.006915 0.016057 0.014284 0.014384 0.003605 0.011854

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.44 0.33 -9.69 -11.73 -11.66 -0.36 -3.93 -9.70 -9.69 -3.25 0.90 -0.66 -11.42 -15.01 -13.49
Bud Neck -6.46 -6.65 -9.68 -2.75 -3.62 -2.45 -0.03 0.55 8.90 0.78 -9.53 -3.15 -5.58 7.40 -3.69
Bud Site -3.49 -3.45 -24.89 -28.76* -25.29* -2.87 -5.66 -17.38 -20.48 -4.62 -4.06 -5.27 -28.85 -35.22* -25.44
Cell Periphery 2.09 2.54 2.04 -0.50 -8.21 2.12 1.26 2.20 0.84 2.87 2.58 2.88 2.52 -0.87 -6.18
Cytoplasm -16.24 -35.20 -29.28 -9.39 17.03 3.25 0.79 -0.17 -12.24 -1.63 -4.96 -22.11 -12.95 -15.27 15.50
Cytoplasmic Foci -30.07* -10.62 -18.09 17.66 -6.18 4.98 3.58 7.99 12.62 6.40 -9.45 0.78 -1.16 21.70 -4.44
Eisosomes -6.20 0.73 -6.14 1.38 -9.88 5.01 2.42 5.56 2.06 5.32 -0.94 3.74 -0.20 1.30 -8.08
Endoplasmic Reticulum -1.12 -16.75 -5.51 -5.32 8.52 0.82 -3.17 -1.78 -7.84 2.52 -0.11 -15.25 -6.18 -8.15 10.44
Endosome -7.42 3.11 -0.74 9.94 -7.41 -0.88 -0.38 1.91 9.87 3.53 -7.31 3.55 0.21 15.50 -7.14
Golgi -3.51 7.28 2.22 11.95 -19.30 -6.37 -0.21 -2.83 6.47 -2.27 -2.37 7.32 1.88 11.73 -20.53
Lipid Particles -1.03 0.21 1.16 6.86 6.86 2.38 2.51 3.57 9.22 4.32 0.39 2.21 3.15 12.40 6.14
Mitochondria 20.88* 37.81* 28.93* 13.93 -27.56* -12.04 2.71 -1.91 23.60 -9.62 11.50 24.02 18.34 25.31 -32.00*
Mitotic Spindle -6.97 -10.03 -16.95 -11.24 -2.07 0.80 0.69 -2.46 -12.61 -9.57 -1.26 -5.71 -10.43 -17.14 -4.21
None -3.88 -12.84 -2.13 0.90 3.39 -1.60 -0.54 0.69 2.51 0.87 -4.34 -4.84 -1.05 2.97 2.94
Nuclear Periphery -1.21 -12.65 -2.20 -0.67 12.36 1.38 0.78 0.40 -3.93 -1.27 0.50 -10.34 -0.89 -3.29 11.10
Nuclear Periphery Foci -0.47 -8.63 -1.27 -1.34 8.82 2.40 -3.69 0.43 -3.28 3.97 1.07 -9.15 -1.10 -3.80 9.20
Nucleolus 0.97 0.02 1.31 4.23 9.14 1.17 1.05 2.08 5.65 3.51 0.89 0.45 1.91 7.31 8.71
Nucleus 0.32 -28.36 -0.14 -1.61 35.49 2.90 -1.48 2.40 -2.22 4.52 1.02 -26.24 0.74 -0.99 33.38
Peroxisomes -7.33 1.10 -12.29 -6.47 -20.10* 3.26 -0.24 2.13 -6.05 3.35 -0.78 2.26 -5.31 -10.71 -15.55
Vacuole -6.38 -20.33 -18.37 -15.39 5.67 -1.40 -5.10 -8.46 -16.99 -1.59 -7.65 -17.04 -20.20 -19.67 0.11
Vacuole Periphery 0.87 4.82 2.93 5.20 -7.95 -1.78 0.94 -1.71 0.07 -4.44 -0.04 5.05 1.45 4.24 -12.29
Standard name
Human Ortholog
Description F1F0 ATP synthase peripheral stalk assembly factor; subunit of the matrix-exposed inner mitochondrial membrane localized INA complex (Ina22p-Ina17p) involved in assembly of the F1F0 peripheral stalk; co-purifies with Aim43p, ATP synthase subunits, and cytochrome bc1 complex assembly factors; interacts with Arh1p, a mitochondrial oxidoreductase; deletion mutant has a respiratory growth defect
Localization
Cell Percentages mitochondrion (74%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Ina22

Ina22


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ina22-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available