Standard name
Human Ortholog
Description Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins; human ISCA1 can complement isa1 null mutant

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.09 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.17 0.18 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0.05 0.06 0.05 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Golgi 0 0.09 0.3 0.2 0 0 0.06 0 0 0 0 0 0.23 0.36 0.26 0.5 0.49 0.06 0.09 0 0.1 0.1 0.08
Mitochondria 0.93 0.91 0.78 0.9 0.85 0.77 0.75 0.75 0.92 0.84 0.89 0.92 0.87 0.81 0.79 0 0 0.77 0.82 0.87 0.77 0.68 0.74
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0.05 0.05 0 0 0.09 0.09 0.08 0.08 0.11 0.1 0.08 0 0.07 0 0 0.09 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0.08 0.1 0.1 0.09 0.08 0.08 0.11 0.08 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.05 0 0 0 0 0 0 0 0 0 0.07 0 0.06 0 0 0 0 0 0 0.05 0.05
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.08 0.09 0.05 0.1 0.09 0.08 0.12 0 0 0.05 0 0.12 0.07 0.11 0.07 0.14 0 0 0 0 0.07 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 4 9 3 0 4 1 0 2 2 0 2 4 1 2 2 3 2 4 2 10 11
Bud 0 0 2 6 6 6 6 4 14 4 7 9 3 0 1 1 1 0 0 0 0 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 3 3 2 0 1 1 3 3 0 0 0 0 1
Cell Periphery 0 7 12 7 2 0 2 4 3 2 0 2 6 8 11 6 5 0 0 0 0 0 1
Cytoplasm 2 5 1 4 6 11 7 13 5 7 5 5 1 7 5 8 10 0 0 0 0 0 0
Endoplasmic Reticulum 2 1 1 1 3 1 0 0 0 2 0 2 0 2 0 1 4 0 1 0 0 1 1
Endosome 1 1 7 1 7 15 25 23 3 8 3 6 2 8 12 1 3 2 1 4 1 8 5
Golgi 1 16 78 55 11 13 23 17 1 10 4 5 48 133 98 23 28 4 15 11 15 34 26
Mitochondria 56 157 200 248 213 246 298 349 367 308 297 404 186 296 293 2 1 62 143 245 122 222 254
Nucleus 0 1 0 1 2 1 7 3 4 1 1 0 0 0 0 2 1 0 0 0 0 0 2
Nuclear Periphery 1 9 12 9 8 28 35 39 32 39 34 33 7 24 11 2 5 1 0 1 1 2 4
Nucleolus 4 2 11 6 20 32 38 41 34 30 36 34 2 9 6 0 1 0 1 0 1 3 1
Peroxisomes 1 0 12 10 2 8 6 9 5 7 2 6 15 13 23 2 0 1 4 12 5 15 16
SpindlePole 1 0 1 2 5 12 13 16 3 12 3 6 0 5 0 1 1 0 0 0 0 1 0
Vac/Vac Membrane 6 13 24 14 24 28 31 56 17 7 15 14 25 25 41 3 8 1 4 1 4 23 7
Unique Cell Count 60 173 258 275 251 320 399 468 401 365 332 438 213 366 370 46 57 81 176 283 159 329 342
Labelled Cell Count 75 214 365 373 315 404 497 575 489 440 412 529 297 534 502 54 71 81 176 283 159 329 342


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.3 11.5 12.9 12.1 8.7 9.8 9.7 9.7 8.5 9.0 8.5 9.1 13.3 12.1 11.7 11.6 11.8 13.4 14.2 14.1
Std Deviation (1e-4) 2.8 2.5 2.3 2.2 2.3 2.0 2.1 1.8 1.9 2.2 2.0 2.4 2.4 2.3 2.3 2.9 3.2 3.2 3.3 3.5
Intensity Change (Log2) -0.09 -0.56 -0.4 -0.41 -0.41 -0.61 -0.52 -0.6 -0.51 0.04 -0.1 -0.14 -0.16 -0.13 0.06 0.14 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.3 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 2.2 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.3 -2.7 -3.9 -3.6 -3.3 -3.3 -3.4 -4.0 -3.8 -1.0 -1.7 -1.1 0 0
Cytoplasm 0 0 2.6 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.1 1.2 2.1 1.4 0 -0.4 0 0 0 -0.4 0.4 0 0
Golgi -2.7 -7.7 -8.6 -8.5 -10.2 -11.6 -9.7 -10.1 -11.4 -1.9 1.6 -1.0 2.6 2.7
Mitochondria 4.0 2.1 -0.2 -0.8 -0.9 5.1 2.2 3.9 5.5 2.8 1.0 0.5 -9.7 -10.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -0.8 -0.8 1.9 2.0 1.9 1.7 2.7 2.5 1.5 -0.7 1.0 -1.1 0 0
Nucleolus -1.4 1.7 2.6 2.5 2.2 2.1 2.0 2.9 1.8 -2.2 -1.3 -2.0 0 0
Peroxisomes -0.6 -2.7 -1.4 -2.4 -2.1 -2.7 -2.0 -3.2 -2.6 1.1 -0.7 0.8 0 0
SpindlePole 0 0 2.7 2.5 2.6 0 2.5 0 0 0 0 0 0 0
Vacuole -1.9 0.1 -0.2 -0.7 1.1 -2.6 -4.2 -2.3 -3.4 0.9 -1.1 0.7 0 1.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 15.8838 15.5719 14.5039 15.4757 13.5979 15.0575 27.6915 25.1631 26.3045 24.4667 25.28 25.3545 21.1188 20.791 19.7761 19.9858 19.2082 20.1241
Actin 0.0125 0.0058 0.0177 0 0.0002 0.0003 0.0022 0.0015 0.0006 0.0421 0.0001 0.0003 0.0124 0.0061 0.0022 0.0001 0.0525 0.0001
Bud 0.0002 0.0001 0.0001 0 0 0 0.0001 0 0 0.0001 0 0 0.0001 0.0003 0.0004 0 0.0001 0
Bud Neck 0.0002 0 0.0002 0 0 0 0 0 0 0.0002 0 0 0.0002 0.0001 0.0009 0 0 0
Bud Periphery 0.0004 0.0001 0.0001 0 0 0 0.0002 0 0.0002 0.0001 0 0.0002 0.0004 0.0014 0.0016 0 0.0002 0.0001
Bud Site 0.0014 0.0001 0.0004 0 0 0 0.0001 0.0001 0 0.0009 0 0 0.0003 0.0002 0.0044 0 0.0002 0
Cell Periphery 0.0002 0 0.0001 0 0 0 0.0001 0.0002 0.0002 0 0 0 0.0001 0.0003 0.0004 0 0 0
Cytoplasm 0.0003 0 0.0003 0 0 0 0.0017 0 0.0002 0.0006 0 0 0.0003 0 0.0002 0 0.0001 0
Cytoplasmic Foci 0.0028 0.0003 0.002 0 0.002 0.0001 0.0029 0.0005 0.0008 0.0097 0 0 0.0052 0.0005 0.0023 0 0.0027 0
Eisosomes 0.0007 0.0002 0.0002 0.0001 0 0.0001 0.0002 0.0001 0.0005 0.0002 0.0001 0.0001 0.0004 0.0002 0.0002 0 0.0003 0.0001
Endoplasmic Reticulum 0.0005 0 0.0003 0 0 0 0.0003 0 0.0004 0.0002 0 0 0.0005 0 0.0001 0 0.0001 0
Endosome 0.0058 0.0008 0.0031 0.0001 0.0022 0.0008 0.0019 0.0012 0.0025 0.0027 0 0.0001 0.0031 0.0019 0.002 0.0002 0.0017 0.0005
Golgi 0.0065 0.0055 0.0036 0.0002 0.0029 0.0063 0.0038 0.004 0.0007 0.0037 0.0001 0.0007 0.0026 0.0081 0.0109 0.0002 0.0102 0.0023
Lipid Particles 0.0155 0.0033 0.0053 0.0006 0.0339 0.0037 0.0051 0.0284 0.0018 0.0043 0.0013 0.0018 0.0144 0.0083 0.005 0.0003 0.0038 0.0025
Mitochondria 0.9372 0.9776 0.9567 0.9982 0.9525 0.9788 0.9734 0.9445 0.987 0.9051 0.9936 0.9911 0.9327 0.9445 0.9538 0.9929 0.8912 0.9922
None 0.0006 0 0.0006 0 0 0 0.0007 0 0.0001 0.0023 0 0 0.0006 0 0.0007 0 0.0006 0
Nuclear Periphery 0.0009 0 0.0006 0 0 0.0001 0.0007 0.0001 0.0006 0.0003 0 0.0001 0.0046 0.0001 0.0001 0 0.0001 0
Nucleolus 0.0006 0 0.0002 0 0 0.0001 0.0001 0.0005 0.0003 0 0 0 0.0005 0.0006 0.0001 0 0 0
Nucleus 0.0005 0 0.0002 0 0 0 0.0001 0.0001 0.0002 0 0 0 0.0004 0.0001 0.0001 0 0 0
Peroxisomes 0.0056 0.0033 0.003 0.0004 0.0021 0.0005 0.0024 0.0019 0.0003 0.0249 0.0001 0.0021 0.0054 0.003 0.0035 0.0002 0.0352 0.0003
Punctate Nuclear 0.0006 0 0.0015 0 0.0001 0 0.0005 0 0.0002 0.0022 0 0 0.0011 0 0.0001 0 0.0006 0
Vacuole 0.0016 0.0002 0.0006 0 0.0001 0.0006 0.0007 0.0016 0.002 0 0.0002 0.0001 0.002 0.0044 0.0017 0.0006 0 0.0003
Vacuole Periphery 0.0054 0.0026 0.0032 0.0003 0.0039 0.0086 0.0029 0.0151 0.0016 0.0001 0.0044 0.0031 0.0128 0.0199 0.0093 0.0053 0.0003 0.0014

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 49.089 43.2276 34.9928 34.821 41.7974 54.1877 49.9986 47.3781 54.5948 44.6396
Translational Efficiency 1.0762 1.232 1.3019 1.6357 1.4184 1.5989 1.4841 1.5521 1.2482 1.2727

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1336 1539 2172 1921 1552 1367 1309 2019 2888 2906 3481 3940

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 904.51 1343.92 1100.66 1205.09 914.37 1353.50 1008.50 1192.08 909.81 1348.43 1066.00 1198.42
Standard Deviation 180.19 271.88 169.69 177.50 168.84 251.13 160.73 191.92 174.25 262.37 172.26 185.15
Intensity Change Log 2 0.571239 0.283161 0.413933 0.565845 0.141361 0.382631 0.568530 0.213618 0.398282

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.004786 0.003359 0.002850 0.005951 0.001638 0.003612 0.004210 0.004876 0.003094 0.003478 0.003361 0.005400
Bud Neck 0.002569 0.001282 0.001473 0.001082 0.000869 0.000816 0.006039 0.000983 0.001655 0.001063 0.003190 0.001032
Bud Site 0.006422 0.001677 0.004146 0.004751 0.001687 0.000669 0.012764 0.004550 0.003878 0.001203 0.007387 0.004648
Cell Periphery 0.000508 0.000090 0.000178 0.000270 0.000156 0.000076 0.000689 0.000261 0.000319 0.000084 0.000370 0.000266
Cytoplasm 0.000974 0.000486 0.001276 0.000612 0.000386 0.000223 0.002949 0.000797 0.000658 0.000363 0.001905 0.000707
Cytoplasmic Foci 0.010576 0.023010 0.030875 0.007304 0.015682 0.013389 0.039572 0.008839 0.013320 0.018484 0.034145 0.008090
Eisosomes 0.000506 0.000406 0.000068 0.000118 0.000092 0.000274 0.000090 0.000096 0.000284 0.000344 0.000076 0.000107
Endoplasmic Reticulum 0.000461 0.000161 0.000290 0.000336 0.000264 0.000110 0.000665 0.000221 0.000355 0.000137 0.000431 0.000277
Endosome 0.006412 0.007332 0.037603 0.008003 0.010569 0.004391 0.054017 0.009427 0.008646 0.005948 0.043775 0.008733
Golgi 0.011634 0.038202 0.050479 0.026031 0.022245 0.027901 0.036645 0.028386 0.017336 0.033356 0.045277 0.027238
Lipid Particles 0.007342 0.008338 0.008898 0.000592 0.010737 0.007211 0.012770 0.002163 0.009167 0.007808 0.010354 0.001397
Mitochondria 0.906268* 0.885340* 0.806107* 0.919397* 0.904214* 0.922625* 0.739563* 0.908878* 0.905164* 0.902879* 0.781084* 0.914006*
Mitotic Spindle 0.000705 0.001953 0.009483 0.003896 0.001587 0.000343 0.022588 0.003021 0.001179 0.001196 0.014411 0.003448
None 0.001287 0.001170 0.000433 0.000376 0.000462 0.000917 0.000629 0.000627 0.000844 0.001051 0.000507 0.000505
Nuclear Periphery 0.000084 0.000311 0.001018 0.000109 0.000234 0.000186 0.001849 0.000387 0.000164 0.000252 0.001330 0.000252
Nuclear Periphery Foci 0.000497 0.000323 0.000952 0.000151 0.000410 0.000157 0.002154 0.000519 0.000450 0.000245 0.001404 0.000339
Nucleolus 0.001711 0.000416 0.000466 0.000083 0.000242 0.000164 0.000993 0.000076 0.000922 0.000297 0.000664 0.000080
Nucleus 0.000713 0.000084 0.000175 0.000060 0.000123 0.000047 0.000886 0.000053 0.000396 0.000066 0.000442 0.000056
Peroxisomes 0.031841 0.016383 0.015925 0.009963 0.022514 0.011906 0.031775 0.006603 0.026829 0.014277 0.021885 0.008241
Vacuole 0.002405 0.005092 0.011916 0.004751 0.002734 0.002290 0.015069 0.006968 0.002582 0.003774 0.013102 0.005887
Vacuole Periphery 0.002299 0.004585 0.015391 0.006165 0.003156 0.002692 0.014086 0.012269 0.002759 0.003695 0.014900 0.009293

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.43 1.98 -0.91 -2.60 -3.19 -2.99 -2.98 -3.34 -1.13 -0.53 -0.66 -0.45 -2.93 -2.58 -2.69
Bud Neck 2.54 2.20 3.00 0.91 1.96 0.25 -6.73 -0.52 -0.78 6.52 2.26 -4.05 2.35 0.16 6.72
Bud Site 3.23 1.45 1.04 -3.22 -0.55 1.83 -5.99 -2.83 -4.40 4.14 3.54 -3.28 -0.88 -5.25 2.68
Cell Periphery 3.92 3.03 2.10 -4.27 -1.95 2.76 -2.21 -2.50 -5.25 1.77 4.54 -0.50 0.93 -6.65 1.10
Cytoplasm 1.41 -0.66 1.08 -1.07 1.95 2.07 -3.35 -2.33 -3.39 2.75 1.76 -3.23 -0.31 -3.28 3.26
Cytoplasmic Foci -6.66 -10.34 2.56 9.29 13.11 1.38 -8.35 4.68 3.26 11.27 -4.05 -12.71 5.32 9.31 17.11
Eisosomes 1.28 6.03 5.26 8.91 -3.90 -7.24 0.25 -0.34 6.68 -0.52 -1.54 6.08 5.05 11.18 -3.54
Endoplasmic Reticulum 2.87 1.63 0.97 -2.14 -0.55 3.88 -5.48 0.93 -3.43 6.38 4.12 -1.36 1.19 -3.23 3.16
Endosome -0.77 -13.24 -1.46 -0.60 12.67 5.64 -13.31 0.89 -5.14 13.81 3.26 -18.24 -0.12 -3.65 18.43
Golgi -9.09 -13.78 -6.00 3.66 7.56 -1.87 -4.73 -2.18 -0.15 2.54 -7.51 -12.97 -5.28 2.63 7.70
Lipid Particles -0.66 -1.08 6.09 7.47 8.66 2.20 -1.15 7.09 4.19 7.00 1.24 -1.09 9.40 8.09 10.70
Mitochondria 2.47 11.42 -1.75 -4.66 -14.75 -2.47 15.23 -0.61 2.06 -16.31 0.40 18.54 -1.67 -2.18 -21.50
Mitotic Spindle -2.18 -7.46 -5.08 -2.33 4.23 3.79 -9.05 -2.13 -4.46 8.24 -0.06 -11.50 -4.87 -4.32 9.04
None 0.75 6.35 6.88 9.20 2.23 -5.10 -1.23 -4.06 3.15 0.13 -2.37 3.83 5.19 8.63 0.19
Nuclear Periphery -3.91 -4.83 -1.18 3.45 4.70 0.50 -3.76 -1.23 -2.04 3.39 -1.49 -5.71 -1.32 -0.00 5.28
Nuclear Periphery Foci 0.77 -1.51 1.55 2.08 3.68 2.47 -3.94 -0.52 -1.69 3.39 1.72 -4.06 0.65 -0.84 4.47
Nucleolus 2.19 1.83 2.79 3.16 1.49 1.12 -3.47 2.96 1.89 4.30 2.25 0.60 3.10 3.64 3.17
Nucleus 2.17 1.78 2.25 0.93 2.03 2.55 -4.44 2.31 -0.41 4.87 2.43 -0.41 2.51 0.62 5.24
Peroxisomes 4.54 4.86 6.79 2.97 3.08 3.65 -2.66 6.35 2.79 9.22 5.65 2.13 9.22 4.19 8.89
Vacuole -3.49 -9.34 -3.32 0.35 6.15 0.79 -9.37 -4.80 -5.44 5.49 -2.43 -12.96 -5.79 -3.25 7.83
Vacuole Periphery -3.32 -12.51 -4.67 -1.77 7.73 0.98 -8.55 -7.45 -7.99 1.05 -2.20 -15.10 -8.75 -7.36 5.46
Standard name
Human Ortholog
Description Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins; human ISCA1 can complement isa1 null mutant
Localization
Cell Percentages mitochondrion (97%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Isa1

Isa1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Isa1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available