Standard name | |
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Human Ortholog | |
Description | Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins; human ISCA1 can complement isa1 null mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | 4 | 9 | 3 | 0 | 4 | 1 | 0 | 2 | 2 | 0 | 2 | 4 | 1 | – | 2 | 2 | 3 | 2 | 4 | 2 | 10 | 11 |
Bud | 0 | 0 | 2 | 6 | 6 | 6 | 6 | 4 | 14 | 4 | 7 | 9 | 3 | 0 | 1 | – | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 3 | 3 | 2 | 0 | 1 | 1 | 3 | 3 | 0 | 0 | 0 | – | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 7 | 12 | 7 | 2 | 0 | 2 | 4 | 3 | 2 | 0 | 2 | 6 | 8 | 11 | – | 6 | 5 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 2 | 5 | 1 | 4 | 6 | 11 | 7 | 13 | 5 | 7 | 5 | 5 | 1 | 7 | 5 | – | 8 | 10 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 2 | 1 | 1 | 1 | 3 | 1 | 0 | 0 | 0 | 2 | 0 | 2 | 0 | 2 | 0 | – | 1 | 4 | 0 | 1 | 0 | 0 | 1 | 1 |
Endosome | 1 | 1 | 7 | 1 | 7 | 15 | 25 | 23 | 3 | 8 | 3 | 6 | 2 | 8 | 12 | – | 1 | 3 | 2 | 1 | 4 | 1 | 8 | 5 |
Golgi | 1 | 16 | 78 | 55 | 11 | 13 | 23 | 17 | 1 | 10 | 4 | 5 | 48 | 133 | 98 | – | 23 | 28 | 4 | 15 | 11 | 15 | 34 | 26 |
Mitochondria | 56 | 157 | 200 | 248 | 213 | 246 | 298 | 349 | 367 | 308 | 297 | 404 | 186 | 296 | 293 | – | 2 | 1 | 62 | 143 | 245 | 122 | 222 | 254 |
Nucleus | 0 | 1 | 0 | 1 | 2 | 1 | 7 | 3 | 4 | 1 | 1 | 0 | 0 | 0 | 0 | – | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 2 |
Nuclear Periphery | 1 | 9 | 12 | 9 | 8 | 28 | 35 | 39 | 32 | 39 | 34 | 33 | 7 | 24 | 11 | – | 2 | 5 | 1 | 0 | 1 | 1 | 2 | 4 |
Nucleolus | 4 | 2 | 11 | 6 | 20 | 32 | 38 | 41 | 34 | 30 | 36 | 34 | 2 | 9 | 6 | – | 0 | 1 | 0 | 1 | 0 | 1 | 3 | 1 |
Peroxisomes | 1 | 0 | 12 | 10 | 2 | 8 | 6 | 9 | 5 | 7 | 2 | 6 | 15 | 13 | 23 | – | 2 | 0 | 1 | 4 | 12 | 5 | 15 | 16 |
SpindlePole | 1 | 0 | 1 | 2 | 5 | 12 | 13 | 16 | 3 | 12 | 3 | 6 | 0 | 5 | 0 | – | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | 6 | 13 | 24 | 14 | 24 | 28 | 31 | 56 | 17 | 7 | 15 | 14 | 25 | 25 | 41 | – | 3 | 8 | 1 | 4 | 1 | 4 | 23 | 7 |
Unique Cell Count | 60 | 173 | 258 | 275 | 251 | 320 | 399 | 468 | 401 | 365 | 332 | 438 | 213 | 366 | 370 | 46 | 57 | 81 | 176 | 283 | 159 | 329 | 342 | |
Labelled Cell Count | 75 | 214 | 365 | 373 | 315 | 404 | 497 | 575 | 489 | 440 | 412 | 529 | 297 | 534 | 502 | 54 | 71 | 81 | 176 | 283 | 159 | 329 | 342 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.3 | 11.5 | 12.9 | 12.1 | 8.7 | 9.8 | 9.7 | 9.7 | 8.5 | 9.0 | 8.5 | 9.1 | 13.3 | 12.1 | 11.7 | – | 11.6 | 11.8 | 13.4 | 14.2 | 14.1 |
Std Deviation (1e-4) | 2.8 | 2.5 | 2.3 | 2.2 | 2.3 | 2.0 | 2.1 | 1.8 | 1.9 | 2.2 | 2.0 | 2.4 | 2.4 | 2.3 | 2.3 | – | 2.9 | 3.2 | 3.2 | 3.3 | 3.5 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.56 | -0.4 | -0.41 | -0.41 | -0.61 | -0.52 | -0.6 | -0.51 | 0.04 | -0.1 | -0.14 | – | -0.16 | -0.13 | 0.06 | 0.14 | 0.13 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cell Periphery | -1.3 | -2.7 | -3.9 | -3.6 | -3.3 | -3.3 | -3.4 | -4.0 | -3.8 | -1.0 | -1.7 | -1.1 | – | 0 | 0 |
Cytoplasm | 0 | 0 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Endosome | 0 | 0.1 | 1.2 | 2.1 | 1.4 | 0 | -0.4 | 0 | 0 | 0 | -0.4 | 0.4 | – | 0 | 0 |
Golgi | -2.7 | -7.7 | -8.6 | -8.5 | -10.2 | -11.6 | -9.7 | -10.1 | -11.4 | -1.9 | 1.6 | -1.0 | – | 2.6 | 2.7 |
Mitochondria | 4.0 | 2.1 | -0.2 | -0.8 | -0.9 | 5.1 | 2.2 | 3.9 | 5.5 | 2.8 | 1.0 | 0.5 | – | -9.7 | -10.8 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nuclear Periphery | -0.8 | -0.8 | 1.9 | 2.0 | 1.9 | 1.7 | 2.7 | 2.5 | 1.5 | -0.7 | 1.0 | -1.1 | – | 0 | 0 |
Nucleolus | -1.4 | 1.7 | 2.6 | 2.5 | 2.2 | 2.1 | 2.0 | 2.9 | 1.8 | -2.2 | -1.3 | -2.0 | – | 0 | 0 |
Peroxisomes | -0.6 | -2.7 | -1.4 | -2.4 | -2.1 | -2.7 | -2.0 | -3.2 | -2.6 | 1.1 | -0.7 | 0.8 | – | 0 | 0 |
SpindlePole | 0 | 0 | 2.7 | 2.5 | 2.6 | 0 | 2.5 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Vacuole | -1.9 | 0.1 | -0.2 | -0.7 | 1.1 | -2.6 | -4.2 | -2.3 | -3.4 | 0.9 | -1.1 | 0.7 | – | 0 | 1.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 15.8838 | 15.5719 | 14.5039 | 15.4757 | 13.5979 | 15.0575 | 27.6915 | 25.1631 | 26.3045 | 24.4667 | 25.28 | 25.3545 | 21.1188 | 20.791 | 19.7761 | 19.9858 | 19.2082 | 20.1241 |
Actin | 0.0125 | 0.0058 | 0.0177 | 0 | 0.0002 | 0.0003 | 0.0022 | 0.0015 | 0.0006 | 0.0421 | 0.0001 | 0.0003 | 0.0124 | 0.0061 | 0.0022 | 0.0001 | 0.0525 | 0.0001 |
Bud | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0003 | 0.0004 | 0 | 0.0001 | 0 |
Bud Neck | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0002 | 0.0001 | 0.0009 | 0 | 0 | 0 |
Bud Periphery | 0.0004 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0004 | 0.0014 | 0.0016 | 0 | 0.0002 | 0.0001 |
Bud Site | 0.0014 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0009 | 0 | 0 | 0.0003 | 0.0002 | 0.0044 | 0 | 0.0002 | 0 |
Cell Periphery | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0.0003 | 0.0004 | 0 | 0 | 0 |
Cytoplasm | 0.0003 | 0 | 0.0003 | 0 | 0 | 0 | 0.0017 | 0 | 0.0002 | 0.0006 | 0 | 0 | 0.0003 | 0 | 0.0002 | 0 | 0.0001 | 0 |
Cytoplasmic Foci | 0.0028 | 0.0003 | 0.002 | 0 | 0.002 | 0.0001 | 0.0029 | 0.0005 | 0.0008 | 0.0097 | 0 | 0 | 0.0052 | 0.0005 | 0.0023 | 0 | 0.0027 | 0 |
Eisosomes | 0.0007 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0 | 0.0003 | 0.0001 |
Endoplasmic Reticulum | 0.0005 | 0 | 0.0003 | 0 | 0 | 0 | 0.0003 | 0 | 0.0004 | 0.0002 | 0 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Endosome | 0.0058 | 0.0008 | 0.0031 | 0.0001 | 0.0022 | 0.0008 | 0.0019 | 0.0012 | 0.0025 | 0.0027 | 0 | 0.0001 | 0.0031 | 0.0019 | 0.002 | 0.0002 | 0.0017 | 0.0005 |
Golgi | 0.0065 | 0.0055 | 0.0036 | 0.0002 | 0.0029 | 0.0063 | 0.0038 | 0.004 | 0.0007 | 0.0037 | 0.0001 | 0.0007 | 0.0026 | 0.0081 | 0.0109 | 0.0002 | 0.0102 | 0.0023 |
Lipid Particles | 0.0155 | 0.0033 | 0.0053 | 0.0006 | 0.0339 | 0.0037 | 0.0051 | 0.0284 | 0.0018 | 0.0043 | 0.0013 | 0.0018 | 0.0144 | 0.0083 | 0.005 | 0.0003 | 0.0038 | 0.0025 |
Mitochondria | 0.9372 | 0.9776 | 0.9567 | 0.9982 | 0.9525 | 0.9788 | 0.9734 | 0.9445 | 0.987 | 0.9051 | 0.9936 | 0.9911 | 0.9327 | 0.9445 | 0.9538 | 0.9929 | 0.8912 | 0.9922 |
None | 0.0006 | 0 | 0.0006 | 0 | 0 | 0 | 0.0007 | 0 | 0.0001 | 0.0023 | 0 | 0 | 0.0006 | 0 | 0.0007 | 0 | 0.0006 | 0 |
Nuclear Periphery | 0.0009 | 0 | 0.0006 | 0 | 0 | 0.0001 | 0.0007 | 0.0001 | 0.0006 | 0.0003 | 0 | 0.0001 | 0.0046 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 |
Nucleolus | 0.0006 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0.0001 | 0.0005 | 0.0003 | 0 | 0 | 0 | 0.0005 | 0.0006 | 0.0001 | 0 | 0 | 0 |
Nucleus | 0.0005 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0004 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Peroxisomes | 0.0056 | 0.0033 | 0.003 | 0.0004 | 0.0021 | 0.0005 | 0.0024 | 0.0019 | 0.0003 | 0.0249 | 0.0001 | 0.0021 | 0.0054 | 0.003 | 0.0035 | 0.0002 | 0.0352 | 0.0003 |
Punctate Nuclear | 0.0006 | 0 | 0.0015 | 0 | 0.0001 | 0 | 0.0005 | 0 | 0.0002 | 0.0022 | 0 | 0 | 0.0011 | 0 | 0.0001 | 0 | 0.0006 | 0 |
Vacuole | 0.0016 | 0.0002 | 0.0006 | 0 | 0.0001 | 0.0006 | 0.0007 | 0.0016 | 0.002 | 0 | 0.0002 | 0.0001 | 0.002 | 0.0044 | 0.0017 | 0.0006 | 0 | 0.0003 |
Vacuole Periphery | 0.0054 | 0.0026 | 0.0032 | 0.0003 | 0.0039 | 0.0086 | 0.0029 | 0.0151 | 0.0016 | 0.0001 | 0.0044 | 0.0031 | 0.0128 | 0.0199 | 0.0093 | 0.0053 | 0.0003 | 0.0014 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 49.089 | 43.2276 | 34.9928 | 34.821 | 41.7974 | 54.1877 | 49.9986 | 47.3781 | 54.5948 | 44.6396 |
Translational Efficiency | 1.0762 | 1.232 | 1.3019 | 1.6357 | 1.4184 | 1.5989 | 1.4841 | 1.5521 | 1.2482 | 1.2727 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1336 | 1539 | 2172 | 1921 | 1552 | 1367 | 1309 | 2019 | 2888 | 2906 | 3481 | 3940 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 904.51 | 1343.92 | 1100.66 | 1205.09 | 914.37 | 1353.50 | 1008.50 | 1192.08 | 909.81 | 1348.43 | 1066.00 | 1198.42 |
Standard Deviation | 180.19 | 271.88 | 169.69 | 177.50 | 168.84 | 251.13 | 160.73 | 191.92 | 174.25 | 262.37 | 172.26 | 185.15 |
Intensity Change Log 2 | — | 0.571239 | 0.283161 | 0.413933 | — | 0.565845 | 0.141361 | 0.382631 | — | 0.568530 | 0.213618 | 0.398282 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.004786 | 0.003359 | 0.002850 | 0.005951 | 0.001638 | 0.003612 | 0.004210 | 0.004876 | 0.003094 | 0.003478 | 0.003361 | 0.005400 |
Bud Neck | 0.002569 | 0.001282 | 0.001473 | 0.001082 | 0.000869 | 0.000816 | 0.006039 | 0.000983 | 0.001655 | 0.001063 | 0.003190 | 0.001032 |
Bud Site | 0.006422 | 0.001677 | 0.004146 | 0.004751 | 0.001687 | 0.000669 | 0.012764 | 0.004550 | 0.003878 | 0.001203 | 0.007387 | 0.004648 |
Cell Periphery | 0.000508 | 0.000090 | 0.000178 | 0.000270 | 0.000156 | 0.000076 | 0.000689 | 0.000261 | 0.000319 | 0.000084 | 0.000370 | 0.000266 |
Cytoplasm | 0.000974 | 0.000486 | 0.001276 | 0.000612 | 0.000386 | 0.000223 | 0.002949 | 0.000797 | 0.000658 | 0.000363 | 0.001905 | 0.000707 |
Cytoplasmic Foci | 0.010576 | 0.023010 | 0.030875 | 0.007304 | 0.015682 | 0.013389 | 0.039572 | 0.008839 | 0.013320 | 0.018484 | 0.034145 | 0.008090 |
Eisosomes | 0.000506 | 0.000406 | 0.000068 | 0.000118 | 0.000092 | 0.000274 | 0.000090 | 0.000096 | 0.000284 | 0.000344 | 0.000076 | 0.000107 |
Endoplasmic Reticulum | 0.000461 | 0.000161 | 0.000290 | 0.000336 | 0.000264 | 0.000110 | 0.000665 | 0.000221 | 0.000355 | 0.000137 | 0.000431 | 0.000277 |
Endosome | 0.006412 | 0.007332 | 0.037603 | 0.008003 | 0.010569 | 0.004391 | 0.054017 | 0.009427 | 0.008646 | 0.005948 | 0.043775 | 0.008733 |
Golgi | 0.011634 | 0.038202 | 0.050479 | 0.026031 | 0.022245 | 0.027901 | 0.036645 | 0.028386 | 0.017336 | 0.033356 | 0.045277 | 0.027238 |
Lipid Particles | 0.007342 | 0.008338 | 0.008898 | 0.000592 | 0.010737 | 0.007211 | 0.012770 | 0.002163 | 0.009167 | 0.007808 | 0.010354 | 0.001397 |
Mitochondria | 0.906268* | 0.885340* | 0.806107* | 0.919397* | 0.904214* | 0.922625* | 0.739563* | 0.908878* | 0.905164* | 0.902879* | 0.781084* | 0.914006* |
Mitotic Spindle | 0.000705 | 0.001953 | 0.009483 | 0.003896 | 0.001587 | 0.000343 | 0.022588 | 0.003021 | 0.001179 | 0.001196 | 0.014411 | 0.003448 |
None | 0.001287 | 0.001170 | 0.000433 | 0.000376 | 0.000462 | 0.000917 | 0.000629 | 0.000627 | 0.000844 | 0.001051 | 0.000507 | 0.000505 |
Nuclear Periphery | 0.000084 | 0.000311 | 0.001018 | 0.000109 | 0.000234 | 0.000186 | 0.001849 | 0.000387 | 0.000164 | 0.000252 | 0.001330 | 0.000252 |
Nuclear Periphery Foci | 0.000497 | 0.000323 | 0.000952 | 0.000151 | 0.000410 | 0.000157 | 0.002154 | 0.000519 | 0.000450 | 0.000245 | 0.001404 | 0.000339 |
Nucleolus | 0.001711 | 0.000416 | 0.000466 | 0.000083 | 0.000242 | 0.000164 | 0.000993 | 0.000076 | 0.000922 | 0.000297 | 0.000664 | 0.000080 |
Nucleus | 0.000713 | 0.000084 | 0.000175 | 0.000060 | 0.000123 | 0.000047 | 0.000886 | 0.000053 | 0.000396 | 0.000066 | 0.000442 | 0.000056 |
Peroxisomes | 0.031841 | 0.016383 | 0.015925 | 0.009963 | 0.022514 | 0.011906 | 0.031775 | 0.006603 | 0.026829 | 0.014277 | 0.021885 | 0.008241 |
Vacuole | 0.002405 | 0.005092 | 0.011916 | 0.004751 | 0.002734 | 0.002290 | 0.015069 | 0.006968 | 0.002582 | 0.003774 | 0.013102 | 0.005887 |
Vacuole Periphery | 0.002299 | 0.004585 | 0.015391 | 0.006165 | 0.003156 | 0.002692 | 0.014086 | 0.012269 | 0.002759 | 0.003695 | 0.014900 | 0.009293 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.43 | 1.98 | -0.91 | -2.60 | -3.19 | -2.99 | -2.98 | -3.34 | -1.13 | -0.53 | -0.66 | -0.45 | -2.93 | -2.58 | -2.69 |
Bud Neck | 2.54 | 2.20 | 3.00 | 0.91 | 1.96 | 0.25 | -6.73 | -0.52 | -0.78 | 6.52 | 2.26 | -4.05 | 2.35 | 0.16 | 6.72 |
Bud Site | 3.23 | 1.45 | 1.04 | -3.22 | -0.55 | 1.83 | -5.99 | -2.83 | -4.40 | 4.14 | 3.54 | -3.28 | -0.88 | -5.25 | 2.68 |
Cell Periphery | 3.92 | 3.03 | 2.10 | -4.27 | -1.95 | 2.76 | -2.21 | -2.50 | -5.25 | 1.77 | 4.54 | -0.50 | 0.93 | -6.65 | 1.10 |
Cytoplasm | 1.41 | -0.66 | 1.08 | -1.07 | 1.95 | 2.07 | -3.35 | -2.33 | -3.39 | 2.75 | 1.76 | -3.23 | -0.31 | -3.28 | 3.26 |
Cytoplasmic Foci | -6.66 | -10.34 | 2.56 | 9.29 | 13.11 | 1.38 | -8.35 | 4.68 | 3.26 | 11.27 | -4.05 | -12.71 | 5.32 | 9.31 | 17.11 |
Eisosomes | 1.28 | 6.03 | 5.26 | 8.91 | -3.90 | -7.24 | 0.25 | -0.34 | 6.68 | -0.52 | -1.54 | 6.08 | 5.05 | 11.18 | -3.54 |
Endoplasmic Reticulum | 2.87 | 1.63 | 0.97 | -2.14 | -0.55 | 3.88 | -5.48 | 0.93 | -3.43 | 6.38 | 4.12 | -1.36 | 1.19 | -3.23 | 3.16 |
Endosome | -0.77 | -13.24 | -1.46 | -0.60 | 12.67 | 5.64 | -13.31 | 0.89 | -5.14 | 13.81 | 3.26 | -18.24 | -0.12 | -3.65 | 18.43 |
Golgi | -9.09 | -13.78 | -6.00 | 3.66 | 7.56 | -1.87 | -4.73 | -2.18 | -0.15 | 2.54 | -7.51 | -12.97 | -5.28 | 2.63 | 7.70 |
Lipid Particles | -0.66 | -1.08 | 6.09 | 7.47 | 8.66 | 2.20 | -1.15 | 7.09 | 4.19 | 7.00 | 1.24 | -1.09 | 9.40 | 8.09 | 10.70 |
Mitochondria | 2.47 | 11.42 | -1.75 | -4.66 | -14.75 | -2.47 | 15.23 | -0.61 | 2.06 | -16.31 | 0.40 | 18.54 | -1.67 | -2.18 | -21.50 |
Mitotic Spindle | -2.18 | -7.46 | -5.08 | -2.33 | 4.23 | 3.79 | -9.05 | -2.13 | -4.46 | 8.24 | -0.06 | -11.50 | -4.87 | -4.32 | 9.04 |
None | 0.75 | 6.35 | 6.88 | 9.20 | 2.23 | -5.10 | -1.23 | -4.06 | 3.15 | 0.13 | -2.37 | 3.83 | 5.19 | 8.63 | 0.19 |
Nuclear Periphery | -3.91 | -4.83 | -1.18 | 3.45 | 4.70 | 0.50 | -3.76 | -1.23 | -2.04 | 3.39 | -1.49 | -5.71 | -1.32 | -0.00 | 5.28 |
Nuclear Periphery Foci | 0.77 | -1.51 | 1.55 | 2.08 | 3.68 | 2.47 | -3.94 | -0.52 | -1.69 | 3.39 | 1.72 | -4.06 | 0.65 | -0.84 | 4.47 |
Nucleolus | 2.19 | 1.83 | 2.79 | 3.16 | 1.49 | 1.12 | -3.47 | 2.96 | 1.89 | 4.30 | 2.25 | 0.60 | 3.10 | 3.64 | 3.17 |
Nucleus | 2.17 | 1.78 | 2.25 | 0.93 | 2.03 | 2.55 | -4.44 | 2.31 | -0.41 | 4.87 | 2.43 | -0.41 | 2.51 | 0.62 | 5.24 |
Peroxisomes | 4.54 | 4.86 | 6.79 | 2.97 | 3.08 | 3.65 | -2.66 | 6.35 | 2.79 | 9.22 | 5.65 | 2.13 | 9.22 | 4.19 | 8.89 |
Vacuole | -3.49 | -9.34 | -3.32 | 0.35 | 6.15 | 0.79 | -9.37 | -4.80 | -5.44 | 5.49 | -2.43 | -12.96 | -5.79 | -3.25 | 7.83 |
Vacuole Periphery | -3.32 | -12.51 | -4.67 | -1.77 | 7.73 | 0.98 | -8.55 | -7.45 | -7.99 | 1.05 | -2.20 | -15.10 | -8.75 | -7.36 | 5.46 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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