Standard name | |
---|---|
Human Ortholog | |
Description | Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 5 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 4 | 7 | 6 | 3 | 3 | 1 | 6 | 7 | 2 | 5 | 18 | 24 |
Bud | 2 | 3 | 1 | 1 | 1 | 5 | 4 | 2 | 6 | 9 | 2 | 5 | 2 | 2 | 2 | 6 | 3 | 6 | 3 | 1 | 0 | 1 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 5 | – | – | – | – | – | – |
Cell Periphery | 15 | 24 | 10 | 4 | 4 | 0 | 4 | 2 | 6 | 5 | 2 | 1 | 17 | 36 | 27 | 22 | 10 | 8 | 0 | 2 | 0 | 0 | 0 | 2 |
Cytoplasm | 1 | 2 | 2 | 6 | 9 | 9 | 14 | 17 | 11 | 12 | 2 | 5 | 5 | 8 | 12 | 6 | 3 | 4 | 0 | 1 | 0 | 0 | 0 | 2 |
Endoplasmic Reticulum | 0 | 12 | 1 | 0 | 1 | 4 | 5 | 1 | 3 | 0 | 4 | 2 | 6 | 1 | 14 | 6 | 3 | 2 | 0 | 1 | 0 | 0 | 1 | 5 |
Endosome | 16 | 6 | 11 | 7 | 13 | 3 | 35 | 25 | 5 | 17 | 5 | 2 | 17 | 31 | 23 | 6 | 11 | 14 | 18 | 16 | 4 | 12 | 22 | 21 |
Golgi | 6 | 27 | 55 | 38 | 35 | 0 | 11 | 11 | 2 | 3 | 1 | 6 | 69 | 129 | 266 | 56 | 61 | 13 | 19 | 32 | 8 | 22 | 43 | 57 |
Mitochondria | 423 | 401 | 130 | 164 | 85 | 243 | 229 | 295 | 302 | 281 | 179 | 191 | 422 | 507 | 535 | 10 | 21 | 12 | 403 | 364 | 155 | 229 | 471 | 452 |
Nucleus | 2 | 3 | 0 | 0 | 1 | 2 | 6 | 3 | 5 | 3 | 0 | 2 | 2 | 0 | 2 | 4 | 3 | 6 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 39 | 15 | 8 | 2 | 12 | 27 | 23 | 32 | 33 | 23 | 20 | 14 | 25 | 13 | 29 | 13 | 21 | 10 | 4 | 1 | 0 | 2 | 2 | 7 |
Nucleolus | 26 | 8 | 4 | 4 | 2 | 17 | 15 | 20 | 18 | 10 | 12 | 8 | 9 | 4 | 8 | 3 | 4 | 5 | 2 | 1 | 0 | 0 | 2 | 3 |
Peroxisomes | 27 | 9 | 10 | 16 | 6 | 1 | 10 | 3 | 6 | 4 | 1 | 1 | 26 | 46 | 37 | 11 | 15 | 22 | 40 | 9 | 18 | 31 | 56 | 46 |
SpindlePole | 9 | 3 | 2 | 1 | 6 | 5 | 11 | 6 | 3 | 7 | 1 | 7 | 6 | 7 | 4 | 18 | 10 | 7 | 3 | 1 | 1 | 0 | 3 | 2 |
Vac/Vac Membrane | 66 | 59 | 18 | 3 | 21 | 16 | 35 | 25 | 14 | 24 | 7 | 9 | 31 | 46 | 78 | 26 | 47 | 29 | 2 | 26 | 0 | 7 | 10 | 11 |
Unique Cell Count | 481 | 446 | 174 | 188 | 142 | 275 | 315 | 373 | 343 | 325 | 197 | 207 | 456 | 597 | 726 | 162 | 177 | 121 | 509 | 472 | 195 | 319 | 642 | 644 |
Labelled Cell Count | 632 | 577 | 253 | 248 | 196 | 333 | 403 | 445 | 415 | 399 | 236 | 253 | 641 | 837 | 1044 | 190 | 215 | 144 | 509 | 472 | 195 | 319 | 642 | 644 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.9 | 7.4 | 8.5 | 8.2 | 8.7 | 5.6 | 7.8 | 7.6 | 6.8 | 6.9 | 6.8 | 6.6 | 8.6 | 9.3 | 9.5 | 9.0 | 9.5 | 8.5 | 7.5 | 7.7 | 8.2 |
Std Deviation (1e-4) | 1.3 | 1.3 | 1.4 | 1.7 | 1.6 | 1.5 | 1.8 | 1.7 | 1.6 | 1.7 | 1.6 | 1.6 | 1.4 | 1.7 | 1.6 | 1.9 | 2.3 | 2.1 | 1.7 | 1.7 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.06 | 0.02 | -0.6 | -0.13 | -0.17 | -0.33 | -0.3 | -0.32 | -0.37 | 0.01 | 0.13 | 0.15 | 0.08 | 0.15 | -0.01 | -0.18 | -0.14 | -0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.8 | -1.3 | 0 | 0 | 0 | -2.5 | -2.6 | -2.6 | -3.1 | -1.1 | 0.1 | -1.2 | 2.4 | 0 | 0.3 |
Cytoplasm | 0 | 0 | 0 | 2.0 | 2.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -1.1 | 0.9 | -3.1 | 1.7 | 0.2 | -3.0 | -0.5 | -1.8 | -2.9 | -1.4 | -0.6 | -2.0 | -1.1 | 0 | 1.6 |
Golgi | -2.5 | -1.4 | -10.0 | -8.7 | -9.6 | -10.6 | -10.2 | -8.4 | -7.6 | -4.7 | -2.7 | 1.2 | 0.6 | 0.6 | -4.2 |
Mitochondria | 3.0 | -2.8 | 3.8 | -0.5 | 1.1 | 3.9 | 3.3 | 4.2 | 4.7 | 6.1 | 3.1 | -0.3 | -12.7 | -11.9 | -11.0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1.4 | 2.0 | 1.2 | 1.7 | 2.0 | 1.1 | 2.0 | 0.9 | 0.4 | 0 | -0.4 | 1.3 | 2.5 | 1.3 |
Nucleolus | 0 | 0 | 1.9 | 1.3 | 1.6 | 1.6 | 0 | 1.8 | 0.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1.0 | -0.6 | 0 | -1.4 | 0 | -2.5 | 0 | -3.0 | -3.1 | 0 | 0.9 | -0.3 | 0.4 | 1.0 | 3.4 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.9 | 2.3 | 0 |
Vacuole | -3.6 | 1.2 | -1.8 | 0.3 | -1.5 | -2.8 | -1.1 | -2.6 | -2.3 | -1.5 | -1.1 | 0.2 | 1.5 | 3.9 | 3.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.849 | 3.2159 | 2.7825 | 2.1886 | 2.0715 | 2.8678 | 4.4163 | 4.8501 | 4.5476 | 4.7647 | 3.7768 | 4.6626 | 6.3197 | 7.1638 | 6.3874 | 6.1934 | 5.9878 | 6.3359 |
Actin | 0.0023 | 0.0013 | 0.0015 | 0.0006 | 0.0003 | 0.0005 | 0.0076 | 0.0021 | 0.0008 | 0.0005 | 0.0004 | 0.0011 | 0.0091 | 0.0027 | 0.0118 | 0.0006 | 0.0052 | 0.0036 |
Bud | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0012 | 0.0005 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0024 | 0 | 0.0001 | 0.0001 |
Bud Neck | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0002 |
Bud Periphery | 0.0004 | 0.0004 | 0.0016 | 0.0001 | 0.0002 | 0.0004 | 0.0003 | 0.0023 | 0.0006 | 0.0017 | 0.0003 | 0.0002 | 0.0003 | 0.0008 | 0.0027 | 0.0001 | 0.0002 | 0.0003 |
Bud Site | 0.0003 | 0.0009 | 0.0014 | 0 | 0.0001 | 0.0007 | 0.0008 | 0.0068 | 0.005 | 0.0003 | 0.0001 | 0.0001 | 0.0005 | 0.0006 | 0.0022 | 0 | 0.0001 | 0.0002 |
Cell Periphery | 0.0002 | 0.0002 | 0.0023 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0035 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.0001 | 0.0002 | 0.004 | 0 | 0.0001 | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0006 | 0.0003 | 0.0038 | 0 | 0.0003 | 0.0005 |
Cytoplasmic Foci | 0.0026 | 0.0061 | 0.0053 | 0.001 | 0.0025 | 0.002 | 0.0057 | 0.0076 | 0.0056 | 0.0017 | 0.0047 | 0.0045 | 0.0084 | 0.0113 | 0.0078 | 0.0003 | 0.0064 | 0.0043 |
Eisosomes | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0003 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0007 | 0.0002 | 0.0005 | 0 | 0 | 0.0016 |
Endosome | 0.0155 | 0.0182 | 0.0207 | 0.0035 | 0.0317 | 0.0081 | 0.0196 | 0.0265 | 0.0117 | 0.0117 | 0.0141 | 0.0096 | 0.0305 | 0.0335 | 0.0179 | 0.0022 | 0.0073 | 0.0187 |
Golgi | 0.0143 | 0.0221 | 0.0183 | 0.008 | 0.011 | 0.0112 | 0.0215 | 0.0428 | 0.0196 | 0.01 | 0.0502 | 0.0283 | 0.0328 | 0.0682 | 0.0214 | 0.0042 | 0.0195 | 0.0269 |
Lipid Particles | 0.0114 | 0.0084 | 0.0045 | 0.0093 | 0.0188 | 0.0098 | 0.0149 | 0.0085 | 0.0106 | 0.0013 | 0.0009 | 0.0037 | 0.0229 | 0.0066 | 0.0051 | 0.0005 | 0.0044 | 0.016 |
Mitochondria | 0.9393 | 0.9238 | 0.9241 | 0.9727 | 0.8859 | 0.9583 | 0.9023 | 0.8693 | 0.9214 | 0.9648 | 0.8841 | 0.9419 | 0.8444 | 0.8042 | 0.8798 | 0.9904 | 0.9069 | 0.8883 |
None | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0006 | 0.0001 | 0.001 | 0 | 0.0015 | 0.0007 |
Nuclear Periphery | 0.0002 | 0.0002 | 0 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0 | 0.0001 | 0 | 0.0003 | 0.0011 | 0.0016 | 0.0009 | 0 | 0.0018 | 0.0022 |
Nucleolus | 0.0001 | 0.0005 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0002 |
Nucleus | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0003 | 0.0003 | 0.0012 | 0 | 0.0002 | 0.0004 |
Peroxisomes | 0.0088 | 0.0085 | 0.0126 | 0.0036 | 0.0391 | 0.0053 | 0.0171 | 0.017 | 0.0163 | 0.0019 | 0.0373 | 0.0072 | 0.0413 | 0.0537 | 0.0251 | 0.0008 | 0.0269 | 0.0251 |
Punctate Nuclear | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0.0017 | 0 | 0.0023 | 0 | 0.0178 | 0.0054 |
Vacuole | 0.0014 | 0.0033 | 0.0012 | 0.0002 | 0.004 | 0.0011 | 0.0027 | 0.0062 | 0.0029 | 0.0016 | 0.0017 | 0.0006 | 0.0014 | 0.0055 | 0.0032 | 0.0001 | 0.0003 | 0.0012 |
Vacuole Periphery | 0.0023 | 0.0049 | 0.0013 | 0.0006 | 0.0057 | 0.0013 | 0.0045 | 0.0073 | 0.0042 | 0.0027 | 0.0058 | 0.0021 | 0.0025 | 0.0094 | 0.0061 | 0.0005 | 0.0007 | 0.0039 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.8646 | 12.3739 | 14.4288 | 23.7169 | 20.5106 | 13.8343 | 22.3415 | 19.9043 | 25.8173 | 26.5979 |
Translational Efficiency | 0.8429 | 1.1998 | 0.7964 | 0.5701 | 0.5145 | 1.0043 | 0.8281 | 0.8094 | 0.8261 | 0.639 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1949 | 472 | 1367 | 110 | 908 | 1727 | 430 | 888 | 2857 | 2199 | 1797 | 998 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 814.87 | 913.01 | 1069.22 | 1124.68 | 823.64 | 926.56 | 1023.49 | 1051.75 | 817.66 | 923.65 | 1058.28 | 1059.79 |
Standard Deviation | 109.27 | 143.57 | 157.55 | 183.66 | 120.75 | 140.60 | 130.23 | 179.18 | 113.12 | 141.35 | 152.71 | 181.13 |
Intensity Change Log 2 | — | 0.164061 | 0.391917 | 0.464873 | — | 0.169871 | 0.313411 | 0.352706 | — | 0.166984 | 0.352988 | 0.409579 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000518 | 0.011044 | 0.002161 | 0.021329 | 0.000805 | 0.001009 | 0.008227 | 0.007366 | 0.000609 | 0.003163 | 0.003613 | 0.008905 |
Bud Neck | 0.000791 | 0.002317 | 0.001952 | 0.004482 | 0.002523 | 0.000711 | 0.004544 | 0.001869 | 0.001341 | 0.001055 | 0.002572 | 0.002157 |
Bud Site | 0.001441 | 0.014079 | 0.004399 | 0.022495 | 0.002545 | 0.001512 | 0.018000 | 0.016593 | 0.001792 | 0.004209 | 0.007653 | 0.017244 |
Cell Periphery | 0.000192 | 0.000476 | 0.000163 | 0.001309 | 0.000469 | 0.000134 | 0.000357 | 0.001695 | 0.000280 | 0.000207 | 0.000209 | 0.001653 |
Cytoplasm | 0.000339 | 0.001898 | 0.000894 | 0.001789 | 0.000516 | 0.000595 | 0.001619 | 0.002007 | 0.000395 | 0.000875 | 0.001068 | 0.001983 |
Cytoplasmic Foci | 0.009850 | 0.021974 | 0.050970 | 0.042322 | 0.021217 | 0.017111 | 0.048741 | 0.021089 | 0.013462 | 0.018155 | 0.050437 | 0.023429 |
Eisosomes | 0.000030 | 0.000127 | 0.000056 | 0.000246 | 0.000044 | 0.000045 | 0.000093 | 0.000104 | 0.000034 | 0.000062 | 0.000065 | 0.000120 |
Endoplasmic Reticulum | 0.000174 | 0.000254 | 0.000539 | 0.001157 | 0.000255 | 0.000127 | 0.000445 | 0.001157 | 0.000200 | 0.000154 | 0.000516 | 0.001157 |
Endosome | 0.028550 | 0.057740 | 0.045759 | 0.067150 | 0.030682 | 0.034457 | 0.059136 | 0.040151 | 0.029227 | 0.039454 | 0.048960 | 0.043126 |
Golgi | 0.017099 | 0.044616 | 0.074041 | 0.098311 | 0.026117 | 0.028280 | 0.049468 | 0.069995 | 0.019965 | 0.031786 | 0.068161 | 0.073116 |
Lipid Particles | 0.004014 | 0.002853 | 0.003925 | 0.000578 | 0.009952 | 0.003780 | 0.002720 | 0.000581 | 0.005902 | 0.003581 | 0.003637 | 0.000580 |
Mitochondria | 0.902825* | 0.774025* | 0.720734* | 0.664242* | 0.856277* | 0.877849* | 0.697858* | 0.773013* | 0.888031* | 0.855564* | 0.715260* | 0.761024* |
Mitotic Spindle | 0.001745 | 0.002577 | 0.022503 | 0.017333 | 0.004715 | 0.004476 | 0.022762 | 0.009639 | 0.002689 | 0.004068 | 0.022565 | 0.010487 |
None | 0.000161 | 0.000228 | 0.000385 | 0.000286 | 0.000187 | 0.000181 | 0.000258 | 0.000337 | 0.000169 | 0.000191 | 0.000355 | 0.000332 |
Nuclear Periphery | 0.000091 | 0.000058 | 0.001798 | 0.000223 | 0.000201 | 0.000093 | 0.000203 | 0.000158 | 0.000126 | 0.000086 | 0.001416 | 0.000165 |
Nuclear Periphery Foci | 0.000255 | 0.000105 | 0.001693 | 0.000350 | 0.001028 | 0.000264 | 0.000527 | 0.000177 | 0.000501 | 0.000230 | 0.001414 | 0.000196 |
Nucleolus | 0.000167 | 0.000050 | 0.000327 | 0.000084 | 0.000439 | 0.000229 | 0.000262 | 0.000041 | 0.000253 | 0.000190 | 0.000312 | 0.000045 |
Nucleus | 0.000109 | 0.000078 | 0.000208 | 0.000090 | 0.000263 | 0.000239 | 0.000231 | 0.000068 | 0.000158 | 0.000204 | 0.000214 | 0.000070 |
Peroxisomes | 0.024247 | 0.054582 | 0.013147 | 0.028214 | 0.035380 | 0.020601 | 0.065596 | 0.017392 | 0.027785 | 0.027895 | 0.025698 | 0.018584 |
Vacuole | 0.002645 | 0.005647 | 0.023432 | 0.021293 | 0.002922 | 0.004243 | 0.010129 | 0.017225 | 0.002733 | 0.004544 | 0.020249 | 0.017673 |
Vacuole Periphery | 0.004757 | 0.005271 | 0.030915 | 0.006718 | 0.003464 | 0.004066 | 0.008821 | 0.019345 | 0.004346 | 0.004325 | 0.025628 | 0.017953 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.08 | -5.01 | -2.74 | -1.28 | -2.52 | -0.98 | -3.67 | -4.36 | -4.21 | 0.34 | -4.41 | -5.49 | -5.25 | -3.42 | -3.16 |
Bud Neck | -3.63 | -3.48 | -2.70 | -1.54 | -1.81 | 1.81 | -1.74 | 0.62 | -3.44 | 3.86 | 0.81 | -2.88 | -1.79 | -3.23 | 1.01 |
Bud Site | -3.82 | -3.96 | -2.86 | -1.05 | -2.44 | 1.13 | -4.38 | -5.36 | -6.04 | 0.31 | -2.93 | -5.66 | -6.52 | -5.29 | -3.76 |
Cell Periphery | -4.21 | 1.20 | -2.06 | -1.53 | -2.12 | 1.38 | 0.45 | -2.23 | -3.16 | -2.69 | 0.89 | 0.88 | -3.05 | -3.25 | -3.25 |
Cytoplasm | -2.11 | -4.81 | -2.31 | 0.11 | -1.35 | -0.27 | -4.21 | -3.90 | -3.73 | -1.08 | -2.07 | -5.88 | -5.18 | -3.00 | -2.85 |
Cytoplasmic Foci | -4.28 | -14.13 | -3.55 | -2.14 | 0.91 | 1.73 | -5.31 | 0.05 | -1.67 | 5.34 | -3.22 | -14.46 | -4.45 | -2.23 | 8.53 |
Eisosomes | -7.99 | -9.36 | -3.03 | -1.64 | -2.67 | -0.27 | -6.39 | -5.71 | -5.65 | -0.87 | -7.79 | -10.68 | -7.05 | -4.58 | -4.48 |
Endoplasmic Reticulum | -1.62 | -6.61 | -2.61 | -2.39 | -1.63 | 2.47 | -2.53 | -3.19 | -3.73 | -2.55 | 1.54 | -6.91 | -3.81 | -4.02 | -2.56 |
Endosome | -4.85 | -5.65 | -3.27 | -0.73 | -1.79 | -1.06 | -5.06 | -2.34 | -1.59 | 3.36 | -3.88 | -7.39 | -4.22 | -1.04 | 1.64 |
Golgi | -5.61 | -14.37 | -4.57 | -2.92 | -1.34 | -0.69 | -4.54 | -7.44 | -7.40 | -2.95 | -5.58 | -14.69 | -10.08 | -7.61 | -0.83 |
Lipid Particles | 1.34 | 0.11 | 4.95 | 3.87 | 5.42 | 3.39 | 4.01 | 5.43 | 4.90 | 3.78 | 2.66 | 2.67 | 7.36 | 5.68 | 6.36 |
Mitochondria | 8.28 | 17.75 | 6.58 | 2.83 | 1.52 | -2.07 | 8.29 | 5.89 | 8.48 | -3.74 | 4.51 | 19.01 | 11.08 | 7.92 | -3.48 |
Mitotic Spindle | -1.90 | -9.46 | -3.40 | -3.22 | 1.05 | 0.19 | -4.82 | -2.47 | -3.00 | 3.41 | -1.82 | -10.41 | -5.10 | -4.05 | 5.14 |
None | -2.82 | -10.59 | -3.83 | -1.76 | 3.19 | 0.17 | -2.82 | -4.84 | -3.68 | -2.70 | -0.73 | -11.04 | -6.37 | -3.99 | 0.93 |
Nuclear Periphery | 1.33 | -4.10 | -1.52 | -1.94 | 3.71 | 1.13 | -0.02 | 0.43 | -1.67 | 1.03 | 1.10 | -4.05 | -0.85 | -2.28 | 3.93 |
Nuclear Periphery Foci | 1.54 | -4.09 | -0.47 | -1.40 | 3.51 | 1.75 | 1.14 | 1.95 | 1.23 | 2.10 | 1.73 | -2.89 | 1.96 | 0.55 | 4.65 |
Nucleolus | 1.88 | -1.40 | 1.19 | -0.93 | 2.52 | 1.00 | 1.03 | 2.48 | 1.28 | 2.72 | 0.53 | -0.52 | 3.12 | 1.25 | 3.75 |
Nucleus | 0.85 | -1.85 | 0.47 | -0.37 | 2.43 | 0.20 | 0.43 | 2.49 | 1.22 | 2.66 | -0.37 | -1.09 | 2.31 | 1.21 | 3.74 |
Peroxisomes | -4.09 | 4.12 | -0.41 | 2.27 | -1.60 | 3.55 | -3.66 | 4.01 | 0.98 | 6.13 | -0.02 | 0.70 | 2.91 | 2.77 | 2.16 |
Vacuole | -2.58 | -12.37 | -2.03 | -1.69 | 0.21 | -1.79 | -4.98 | -6.50 | -5.96 | -2.83 | -3.54 | -13.13 | -6.91 | -5.99 | 1.02 |
Vacuole Periphery | -0.48 | -11.99 | -1.10 | -0.75 | 8.78 | -1.01 | -3.61 | -7.70 | -7.41 | -4.26 | 0.04 | -12.48 | -7.36 | -7.36 | 3.12 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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