Standard name | |
---|---|
Human Ortholog | |
Description | Dihydrolipoyl transsuccinylase; component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 2 | 6 | 9 | 1 | 5 | 5 | 17 |
Bud | 10 | 2 | 4 | 2 | 0 | 3 | 3 | 1 | 2 | 3 | 2 | 2 | 2 | 7 | 6 | 10 | 3 | 3 | 1 | 1 | 0 | 0 | 0 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 5 |
Bud Site | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 3 | 1 | 1 | – | – | – | – | – | – |
Cell Periphery | 4 | 5 | 1 | 0 | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 1 | 4 | 6 | 4 | 4 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 12 | 4 | 3 | 4 | 5 | 8 | 16 | 12 | 7 | 7 | 11 | 11 | 8 | 17 | 28 | 18 | 7 | 4 | 4 | 3 | 0 | 0 | 1 | 3 |
Endoplasmic Reticulum | 0 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 2 | 2 | 1 | 0 | 0 | 0 | 1 | 1 | 2 |
Endosome | 43 | 8 | 4 | 1 | 4 | 0 | 3 | 2 | 1 | 0 | 0 | 0 | 15 | 76 | 98 | 42 | 37 | 17 | 83 | 19 | 8 | 29 | 37 | 60 |
Golgi | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 3 | 3 | 9 | 14 | 9 | 19 | 9 | 7 | 14 | 18 | 25 |
Mitochondria | 293 | 101 | 59 | 50 | 68 | 131 | 105 | 121 | 56 | 87 | 93 | 107 | 142 | 238 | 193 | 9 | 7 | 4 | 143 | 61 | 36 | 71 | 66 | 88 |
Nucleus | 0 | 2 | 0 | 0 | 0 | 0 | 3 | 2 | 0 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 3 | 3 | 3 | 3 | 3 | 10 | 14 | 18 | 8 | 7 | 6 | 4 | 3 | 3 | 2 | 2 | 0 | 0 | 2 | 0 | 1 | 1 | 1 | 1 |
Nucleolus | 6 | 5 | 7 | 0 | 3 | 12 | 12 | 24 | 9 | 10 | 10 | 19 | 3 | 1 | 2 | 7 | 6 | 2 | 0 | 1 | 0 | 1 | 1 | 2 |
Peroxisomes | 91 | 13 | 10 | 6 | 6 | 9 | 4 | 6 | 0 | 3 | 0 | 1 | 29 | 123 | 112 | 87 | 46 | 28 | 86 | 17 | 15 | 47 | 45 | 64 |
SpindlePole | 37 | 3 | 2 | 3 | 0 | 2 | 1 | 3 | 1 | 1 | 4 | 0 | 11 | 32 | 27 | 49 | 32 | 17 | 7 | 1 | 0 | 5 | 5 | 12 |
Vac/Vac Membrane | 15 | 7 | 3 | 1 | 4 | 3 | 4 | 2 | 1 | 3 | 2 | 1 | 11 | 24 | 22 | 8 | 9 | 4 | 8 | 7 | 2 | 1 | 3 | 3 |
Unique Cell Count | 361 | 124 | 68 | 58 | 78 | 148 | 135 | 151 | 70 | 104 | 109 | 120 | 182 | 379 | 363 | 186 | 125 | 74 | 373 | 135 | 75 | 184 | 197 | 302 |
Labelled Cell Count | 517 | 160 | 97 | 70 | 94 | 182 | 165 | 194 | 85 | 125 | 129 | 148 | 235 | 533 | 500 | 250 | 167 | 93 | 373 | 135 | 75 | 184 | 197 | 302 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.0 | 6.0 | 6.7 | 6.1 | 8.2 | 7.9 | 8.0 | 9.1 | 10.8 | 10.6 | 10.9 | 12.7 | 5.8 | 5.7 | 5.6 | 6.5 | 7.8 | 8.2 | 6.0 | 6.1 | 6.9 |
Std Deviation (1e-4) | 1.5 | 2.0 | 1.8 | 2.0 | 3.5 | 2.6 | 2.5 | 2.8 | 3.5 | 5.3 | 5.0 | 5.9 | 1.5 | 2.0 | 1.5 | 1.9 | 2.0 | 1.9 | 3.2 | 2.3 | 3.3 |
Intensity Change (Log2) | – | – | – | -0.12 | 0.3 | 0.24 | 0.26 | 0.44 | 0.7 | 0.68 | 0.72 | 0.93 | -0.21 | -0.23 | -0.25 | -0.04 | 0.23 | 0.3 | -0.14 | -0.12 | 0.06 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 1.7 | 0 | 0 | 0 | 1.4 | 1.2 | 0 | 0 | 0 | 1.3 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.6 | 2.8 | 3.8 | 3.1 | 3.8 | 2.9 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 0 |
Mitochondria | -0.1 | 0.1 | 0.4 | -1.5 | -1.2 | -1.1 | -0.6 | -0.3 | 0.5 | -1.5 | -3.9 | -5.2 | -13.1 | -11.4 | -9.7 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1.4 | 1.7 | 1.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | -0.5 | -0.3 | 1.1 | 0.5 | -0.1 | -0.2 | 1.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -0.7 | -1.4 | -2.1 | 0 | 0 | 0 | -2.9 | 0 | 0 | 0.2 | 2.9 | 2.7 | 4.7 | 3.2 | 3.1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.6 | 0 | 4.1 | 3.9 | 3.5 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.2784 | 6.5935 | 6.0577 | 5.8921 | 6.9732 | 5.7673 | 6.9634 | 7.1303 | 7.212 | 6.429 | 6.508 | 6.7683 | 9.9811 | 9.0599 | 8.9858 | 9.1398 | 7.9816 | 9.5446 |
Actin | 0.0352 | 0.0227 | 0.0185 | 0.0003 | 0.0027 | 0.0192 | 0.0412 | 0.0159 | 0.0128 | 0.0003 | 0.0231 | 0.0264 | 0.0486 | 0.012 | 0.0083 | 0.015 | 0.0519 | 0.0103 |
Bud | 0.0004 | 0.0004 | 0.0001 | 0 | 0.0001 | 0.0006 | 0.0003 | 0.0005 | 0.0003 | 0 | 0.0008 | 0.0002 | 0.0006 | 0.0003 | 0.0002 | 0.0002 | 0.0004 | 0.0001 |
Bud Neck | 0.0076 | 0.0004 | 0.0001 | 0 | 0 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0 | 0.0004 | 0.0002 | 0.0085 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 |
Bud Periphery | 0.0007 | 0.0005 | 0.0002 | 0 | 0.0001 | 0.0013 | 0.0005 | 0.0007 | 0.0004 | 0 | 0.0011 | 0.0003 | 0.0011 | 0.0005 | 0.0003 | 0.0003 | 0.0009 | 0.0001 |
Bud Site | 0.0032 | 0.0022 | 0.0003 | 0.0001 | 0.0001 | 0.0004 | 0.0011 | 0.0047 | 0.001 | 0 | 0.0023 | 0.0008 | 0.0126 | 0.001 | 0.0006 | 0.0003 | 0.0009 | 0.0001 |
Cell Periphery | 0.0019 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0012 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.0132 | 0.0001 | 0 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0012 | 0 | 0.0001 | 0.0001 | 0.0029 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 |
Cytoplasmic Foci | 0.0333 | 0.0122 | 0.0057 | 0.0022 | 0.0005 | 0.0041 | 0.0169 | 0.0165 | 0.0092 | 0.0006 | 0.024 | 0.0062 | 0.0142 | 0.013 | 0.0053 | 0.0037 | 0.007 | 0.0024 |
Eisosomes | 0.0006 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0008 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0005 | 0.0003 | 0.0003 | 0.0003 | 0.0007 | 0.0004 |
Endoplasmic Reticulum | 0.0017 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0019 | 0 | 0.0001 | 0.0004 | 0.0007 | 0.0002 |
Endosome | 0.0378 | 0.019 | 0.0081 | 0.0006 | 0.002 | 0.0077 | 0.0222 | 0.0227 | 0.0086 | 0.0002 | 0.0308 | 0.0106 | 0.0257 | 0.0097 | 0.0071 | 0.0105 | 0.0139 | 0.006 |
Golgi | 0.0392 | 0.0551 | 0.0437 | 0.0018 | 0.0052 | 0.0526 | 0.0625 | 0.0712 | 0.0426 | 0.0006 | 0.1183 | 0.0463 | 0.0337 | 0.0463 | 0.037 | 0.0356 | 0.0914 | 0.0329 |
Lipid Particles | 0.0701 | 0.0379 | 0.0246 | 0.0121 | 0.037 | 0.047 | 0.0798 | 0.0182 | 0.0299 | 0.0095 | 0.072 | 0.0368 | 0.0881 | 0.0581 | 0.0373 | 0.0086 | 0.0159 | 0.0232 |
Mitochondria | 0.6209 | 0.7632 | 0.8547 | 0.9499 | 0.9419 | 0.7816 | 0.6553 | 0.746 | 0.8347 | 0.8964 | 0.5346 | 0.817 | 0.6742 | 0.7704 | 0.8441 | 0.8929 | 0.7908 | 0.885 |
None | 0.0174 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0004 | 0 | 0.0006 | 0 | 0.0001 | 0.0002 | 0.0025 | 0 | 0.0001 | 0.0008 | 0.0006 | 0.0002 |
Nuclear Periphery | 0.0041 | 0.0004 | 0.0002 | 0 | 0 | 0.0001 | 0.0005 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0002 | 0.0127 | 0 | 0.0001 | 0.0018 | 0.0018 | 0.001 |
Nucleolus | 0.0125 | 0.0005 | 0.0002 | 0.0001 | 0.0002 | 0.0164 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0012 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0002 |
Nucleus | 0.0028 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0018 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Peroxisomes | 0.0775 | 0.0768 | 0.0325 | 0.0325 | 0.0073 | 0.0639 | 0.1142 | 0.0975 | 0.0508 | 0.0917 | 0.1882 | 0.0509 | 0.0495 | 0.0815 | 0.0559 | 0.0268 | 0.0194 | 0.031 |
Punctate Nuclear | 0.0101 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0036 | 0 | 0.0003 | 0.0008 | 0.0058 | 0.0001 | 0.0002 | 0.001 | 0.0017 | 0.0001 |
Vacuole | 0.0039 | 0.0018 | 0.002 | 0.0001 | 0.0005 | 0.0004 | 0.0009 | 0.0023 | 0.0006 | 0.0001 | 0.001 | 0.0005 | 0.0044 | 0.0027 | 0.0005 | 0.0004 | 0.0003 | 0.0005 |
Vacuole Periphery | 0.0059 | 0.0058 | 0.0086 | 0.0002 | 0.002 | 0.0034 | 0.0019 | 0.0028 | 0.002 | 0.0003 | 0.0019 | 0.0017 | 0.0083 | 0.0028 | 0.0022 | 0.001 | 0.0008 | 0.0062 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 66.1884 | 58.1055 | 54.1589 | 61.5297 | 63.2059 | 70.5091 | 58.2131 | 57.3028 | 68.6578 | 65.616 |
Translational Efficiency | 2.0288 | 1.9668 | 2.1034 | 2.2961 | 2.2605 | 2.2154 | 2.03 | 2.2957 | 2.0872 | 2.0856 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
347 | 42 | 1098 | 767 | 1910 | 176 | 123 | 812 | 2257 | 218 | 1221 | 1579 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 814.61 | 824.34 | 890.61 | 1134.15 | 762.63 | 742.71 | 1522.76 | 1533.26 | 770.62 | 758.44 | 954.29 | 1339.39 |
Standard Deviation | 111.98 | 211.98 | 156.83 | 256.90 | 97.98 | 133.06 | 216.88 | 337.84 | 102.00 | 154.88 | 251.11 | 361.30 |
Intensity Change Log 2 | — | 0.017130 | 0.128684 | 0.477430 | — | -0.038184 | 0.997633 | 1.007547 | — | -0.009351 | 0.613647 | 0.758037 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002341 | 0.015927 | 0.000872 | 0.047093 | 0.001320 | 0.003412 | 0.029253 | 0.003559 | 0.001477 | 0.005823 | 0.003731 | 0.024706 |
Bud Neck | 0.007001 | 0.016358 | 0.022294 | 0.013440 | 0.015730 | 0.009653 | 0.007629 | 0.000058 | 0.014388 | 0.010945 | 0.020816 | 0.006559 |
Bud Site | 0.006392 | 0.064235 | 0.027602 | 0.033204 | 0.024749 | 0.001715 | 0.007333 | 0.000592 | 0.021927 | 0.013760 | 0.025561 | 0.016433 |
Cell Periphery | 0.000406 | 0.000419 | 0.000364 | 0.000461 | 0.000187 | 0.000153 | 0.000363 | 0.000458 | 0.000221 | 0.000204 | 0.000364 | 0.000460 |
Cytoplasm | 0.004752 | 0.019460 | 0.007156 | 0.004523 | 0.041063 | 0.000828 | 0.000941 | 0.000181 | 0.035480 | 0.004418 | 0.006530 | 0.002290 |
Cytoplasmic Foci | 0.054330 | 0.057281 | 0.056675 | 0.030025 | 0.181997* | 0.076542 | 0.096930 | 0.000992 | 0.162369 | 0.072831 | 0.060730 | 0.015095 |
Eisosomes | 0.000110 | 0.001671 | 0.000123 | 0.000867 | 0.000128 | 0.000125 | 0.000391 | 0.000124 | 0.000125 | 0.000423 | 0.000150 | 0.000485 |
Endoplasmic Reticulum | 0.000685 | 0.000459 | 0.001276 | 0.000796 | 0.000676 | 0.000106 | 0.001128 | 0.000173 | 0.000677 | 0.000174 | 0.001261 | 0.000476 |
Endosome | 0.115669 | 0.104225 | 0.169838 | 0.042381 | 0.068006 | 0.181317 | 0.037963 | 0.001528 | 0.075334 | 0.166465 | 0.156553 | 0.021372 |
Golgi | 0.080512 | 0.146045* | 0.041874 | 0.084180 | 0.156965* | 0.111665* | 0.142393* | 0.011380 | 0.145211* | 0.118289* | 0.052000 | 0.046743 |
Lipid Particles | 0.021286 | 0.048585 | 0.014812 | 0.005672 | 0.005508 | 0.024000 | 0.013386 | 0.000245 | 0.007933 | 0.028736 | 0.014668 | 0.002881 |
Mitochondria | 0.580856* | 0.337979* | 0.413331* | 0.612573* | 0.375799* | 0.444168* | 0.549726* | 0.975553* | 0.407325* | 0.423710* | 0.427071* | 0.799236* |
Mitotic Spindle | 0.008509 | 0.014667 | 0.076697 | 0.038203 | 0.030416 | 0.005954 | 0.039984 | 0.000772 | 0.027048 | 0.007632 | 0.072998 | 0.018954 |
None | 0.000568 | 0.000492 | 0.001481 | 0.000630 | 0.000926 | 0.000134 | 0.000838 | 0.000234 | 0.000871 | 0.000203 | 0.001416 | 0.000427 |
Nuclear Periphery | 0.000418 | 0.000036 | 0.002557 | 0.000255 | 0.000138 | 0.000076 | 0.003248 | 0.000025 | 0.000181 | 0.000068 | 0.002627 | 0.000137 |
Nuclear Periphery Foci | 0.001441 | 0.000222 | 0.005097 | 0.000292 | 0.000218 | 0.000147 | 0.002588 | 0.000007 | 0.000406 | 0.000161 | 0.004844 | 0.000146 |
Nucleolus | 0.003031 | 0.000301 | 0.009227 | 0.000463 | 0.000643 | 0.000399 | 0.000642 | 0.000004 | 0.001010 | 0.000380 | 0.008362 | 0.000227 |
Nucleus | 0.003971 | 0.000642 | 0.009426 | 0.000486 | 0.004512 | 0.000598 | 0.000238 | 0.000004 | 0.004429 | 0.000606 | 0.008501 | 0.000238 |
Peroxisomes | 0.092860 | 0.159380* | 0.058451 | 0.063589 | 0.059701 | 0.132942* | 0.026479 | 0.002683 | 0.064799 | 0.138036* | 0.055230 | 0.032268 |
Vacuole | 0.008516 | 0.008567 | 0.053530 | 0.013559 | 0.022107 | 0.003638 | 0.018283 | 0.000500 | 0.020018 | 0.004587 | 0.049980 | 0.006844 |
Vacuole Periphery | 0.006346 | 0.003051 | 0.027316 | 0.007308 | 0.009208 | 0.002428 | 0.020263 | 0.000926 | 0.008768 | 0.002548 | 0.026606 | 0.004026 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.34 | 2.61 | -8.66 | -2.74 | -8.99 | -1.36 | -3.93 | -2.60 | -0.09 | 3.59 | -1.87 | -2.81 | -8.93 | -5.44 | -7.77 |
Bud Neck | -1.19 | -6.27 | -2.83 | 0.36 | 3.71 | 1.49 | 4.17 | 13.74 | 2.56 | 4.83 | 0.94 | -3.41 | 6.11 | 1.28 | 7.90 |
Bud Site | -2.91 | -7.24 | -7.24 | 1.51 | -1.34 | 14.94* | 6.27 | 16.83* | 1.71 | 2.83 | 1.87 | -1.47 | 2.40 | -0.65 | 3.07 |
Cell Periphery | -0.11 | 0.52 | -0.50 | -0.32 | -1.08 | 1.09 | -1.59 | -1.22 | -1.37 | -0.39 | 0.46 | -3.99 | -1.94 | -2.06 | -0.77 |
Cytoplasm | -1.85 | -1.36 | 0.08 | 1.91 | 2.15 | 15.71 | 15.73 | 16.06 | 2.20 | 4.43 | 11.59 | 11.45 | 15.06 | 1.30 | 3.81 |
Cytoplasmic Foci | -0.20 | -0.48 | 5.03 | 2.14 | 7.14 | 12.20 | 6.37 | 42.12 | 9.99 | 7.50 | 11.86 | 21.14 | 36.71 | 8.64 | 14.46 |
Eisosomes | -1.11 | -1.02 | -5.60 | 0.57 | -5.52 | 0.18 | -4.06 | 0.17 | 0.02 | 3.87 | -1.09 | -2.31 | -5.33 | -0.22 | -4.93 |
Endoplasmic Reticulum | 0.88 | -3.12 | -0.31 | -0.91 | 1.56 | 2.67 | -1.41 | 2.21 | -0.80 | 3.77 | 2.70 | -3.06 | 0.81 | -1.82 | 4.37 |
Endosome | 0.39 | -4.95 | 7.82 | 2.20 | 18.66 | -7.06 | 5.59 | 26.38 | 11.33 | 7.66 | -6.40 | -13.14 | 18.03 | 10.28 | 23.24 |
Golgi | -1.99 | 5.01 | -0.36 | 1.90 | -6.28 | 3.71 | 0.74 | 28.87 | 8.79 | 7.13 | 2.26 | 18.59 | 18.82 | 6.26 | 1.11 |
Lipid Particles | -0.91 | 1.52 | 3.78 | 1.45 | 3.77 | -2.80 | -2.06 | 7.08 | 3.66 | 3.58 | -2.66 | -3.34 | 4.56 | 3.33 | 6.18 |
Mitochondria | 4.15 | 7.55 | -1.27 | -4.82 | -11.51 | -2.44 | -5.15 | -71.39* | -19.06 | -12.77 | -0.70 | -1.30 | -35.55* | -14.32 | -27.94* |
Mitotic Spindle | -0.81 | -12.63 | -6.62 | -2.91 | 5.79 | 9.33* | -0.98 | 14.67* | 2.98 | 4.06 | 7.42 | -9.30 | 2.74 | -4.06 | 10.43 |
None | 0.34 | -1.34 | -0.27 | -0.83 | 1.32 | 3.62 | 0.51 | 3.20 | -2.07 | 5.09 | 3.53 | -0.85 | 2.46 | -4.48 | 1.69 |
Nuclear Periphery | 1.75 | -4.98 | 0.73 | -7.73 | 6.16 | 1.71 | -3.08 | 3.23 | 2.12 | 3.19 | 2.49 | -6.93 | 0.96 | -3.22 | 7.10 |
Nuclear Periphery Foci | 2.39 | -3.82 | 2.27 | -0.73 | 5.55 | 1.78 | -2.35 | 6.96 | 4.71 | 2.56 | 2.85 | -5.62 | 3.12 | 0.42 | 5.95 |
Nucleolus | 1.94 | -3.44 | 1.83 | -1.18 | 6.89 | 0.92 | 0.44 | 3.09 | 1.33 | 2.56 | 1.86 | -6.15 | 2.95 | 0.65 | 7.10 |
Nucleus | 1.97 | -2.93 | 2.05 | 0.22 | 6.06 | 5.71 | 8.58 | 9.12 | 1.31 | 3.24 | 6.12 | -3.32 | 8.31 | 0.90 | 6.25 |
Peroxisomes | -1.72 | 3.35 | 2.70 | 2.56 | -0.75 | -4.95 | 5.01 | 19.71 | 8.98 | 3.88 | -5.22 | 2.24 | 8.42 | 7.59 | 5.37 |
Vacuole | -0.02 | -14.96 | -2.62 | -1.74 | 12.49 | 10.99 | 0.85 | 15.89 | 2.89 | 3.82 | 10.25 | -10.80 | 9.25 | -1.90 | 16.27 |
Vacuole Periphery | 2.27 | -10.60 | -0.80 | -3.00 | 10.19 | 9.05 | -1.91 | 13.26 | 3.17 | 3.35 | 9.21 | -10.10 | 6.89 | -2.47 | 12.98 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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