Standard name
Human Ortholog
Description (SGD) Ubiquitin-binding protein; centrally regulates metabolic homeostasis and carbon utilization during nutrient limitation by managing Snf1p activity; subunit of TORC1 rapamycin-sensitive complex involved in growth control; central scaffold that holds the different components of TORC1 together; contains four HEAT repeats and seven WD-40 repeats
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.46 0.65 0.77 0.68 0.71 0.65 0.6 0.66 0.6 – 0.41 0.41 0.45 0.55 0.68 0.61 0.65 0.54 0.05 0.1 0.09 0.09 0.1 0
Endoplasmic Reticulum 0.07 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0.18 0 0 0 0.06 0 0 0.06 0 – 0 0 0 0.06 0 0.15 0.09 0.21 0.19 0.08 0.1 0.15 0.14 0.22
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.09 0.31 0.39 0.23 0.43 0.43 0.27 0.61 – 0.66 0.65 0.12 0 0.18 0.08 0 0 0.05 0.05 0.17 0.05 0 0.05
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.4 0.36 0.1 0.1 0.23 0.16 0.15 0.24 0.09 – 0.11 0.08 0.51 0.43 0.25 0.34 0.31 0.39 0.61 0.71 0.57 0.65 0.66 0.59
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 1 0 0 0 0 0 0 0 – 0 0 0 0 0 1 1 1 0 0 1 0 0 0
Bud 1 1 7 1 3 0 3 0 2 – 0 0 0 2 9 1 0 1 0 1 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 0 0 0 0 1 0 0 – 2 2 0 1 0 1 1 0 0 0 0 0 0 0
Cytoplasm 91 154 104 71 145 81 124 153 74 – 29 29 53 155 193 137 111 100 12 26 13 19 21 8
Endoplasmic Reticulum 13 5 5 0 0 0 1 1 3 – 1 0 5 12 4 8 9 8 7 2 3 2 0 5
Endosome 35 5 1 2 13 4 6 14 0 – 0 0 4 17 1 33 15 39 51 22 15 31 31 65
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 1 0 0 1 5 2 3 0 0 0 2
Mitochondria 9 21 42 41 47 53 88 63 76 – 46 46 14 7 52 18 5 7 13 13 26 10 6 16
Nucleus 1 2 0 1 0 3 2 1 0 – 1 1 0 1 0 2 2 0 0 0 0 2 1 2
Nuclear Periphery 4 6 0 0 0 0 1 0 1 – 1 2 0 2 2 0 0 0 12 2 2 1 1 11
Nucleolus 2 0 0 1 1 0 1 3 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 1 0 0 0 1 – 0 0 0 0 0 1 2 0 0 0 0 0 1 1
Vac/Vac Membrane 79 86 14 11 46 20 31 55 11 – 8 6 61 123 72 77 53 73 161 185 90 141 148 174
Unique Cell Count 198 238 135 105 203 124 207 231 124 70 71 119 283 284 225 172 186 266 262 158 217 224 296
Labelled Cell Count 240 282 173 128 257 161 258 290 168 88 86 137 321 333 279 200 234 266 262 158 217 224 296


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.0 4.9 4.0 4.2 4.6 4.2 4.1 4.7 4.3 – 4.2 4.2 5.2 5.5 4.6 6.2 6.8 7.5 5.6 5.1 5.6
Std Deviation (1e-4) 0.8 1.0 0.8 1.8 1.6 1.5 1.5 2.0 2.0 – 1.8 1.8 1.1 1.0 0.8 1.2 1.6 2.4 1.4 1.2 1.3
Intensity Change (Log2) – – – 0.07 0.21 0.07 0.06 0.22 0.09 – 0.08 0.08 0.39 0.46 0.2 0.64 0.76 0.91 0.49 0.35 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm -1.6 -1.1 -2.1 -3.3 -2.2 -3.0 – -5.1 -5.2 -5.3 -4.4 -1.9 -3.2 -2.4 -4.3
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0.3 0 0 0.6 0.3
Endosome 0 0 0 0 0 0 – 0 0 0 2.5 0 4.4 3.1 5.4
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 -3.7 -8.5 0 -5.7 -6.8 -6.7
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 2.9 1.4 1.2 3.2 -0.4 – 0.2 -0.4 7.1 6.7 3.5 5.0 4.3 5.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0408 0.0048 0.0208 0.0026 0.0001 0.0022 0.0016 0.0013 0.0121 0.0027 0 0 – – – – – –
Bud 0.0013 0.0011 0.0026 0.0028 0.0001 0.0003 0.0022 0.0074 0.0009 0.0002 0.0001 0.0004 – – – – – –
Bud Neck 0.0033 0.0007 0.0046 0.0343 0.0001 0.0032 0.0004 0.0016 0.0006 0.0003 0.0001 0.0003 – – – – – –
Bud Periphery 0.0023 0.0014 0.0036 0.0046 0.0001 0.0007 0.0031 0.0024 0.0007 0.0001 0.0001 0.0005 – – – – – –
Bud Site 0.0083 0.0099 0.0103 0.0098 0.0003 0.0006 0.0016 0.005 0.003 0.0005 0.0001 0.0005 – – – – – –
Cell Periphery 0.0025 0.0006 0.005 0.0019 0.0001 0.0009 0.0005 0.0007 0.0002 0.0001 0 0.0001 – – – – – –
Cytoplasm 0.0654 0.1466 0.1067 0.0719 0.0205 0.074 0.0673 0.1628 0.0706 0.0903 0.0044 0.0433 – – – – – –
Cytoplasmic Foci 0.0509 0.0515 0.0682 0.0472 0.0289 0.0518 0.0666 0.0798 0.1146 0.089 0.1371 0.0449 – – – – – –
Eisosomes 0.0012 0.0001 0.0019 0 0 0.0001 0.0006 0.0001 0 0 0 0 – – – – – –
Endoplasmic Reticulum 0.0104 0.0064 0.0162 0.0028 0.002 0.0042 0.0051 0.0077 0.0043 0.0023 0.0013 0.0067 – – – – – –
Endosome 0.4599 0.47 0.4628 0.4358 0.6248 0.56 0.4249 0.369 0.4724 0.4262 0.7229 0.5247 – – – – – –
Golgi 0.0258 0.0186 0.0202 0.0457 0.0217 0.0162 0.0114 0.0058 0.0382 0.0278 0.0117 0.013 – – – – – –
Lipid Particles 0.0327 0.0059 0.0257 0.0188 0.0028 0.0051 0.0383 0.0213 0.0105 0.0145 0.0086 0.0208 – – – – – –
Mitochondria 0.0402 0.0232 0.025 0.0177 0.0058 0.0169 0.0027 0.0096 0.0114 0.0043 0.0007 0.0024 – – – – – –
None 0.0201 0.0313 0.0251 0.0007 0.0558 0.0088 0.128 0.0372 0.0165 0.0052 0.0002 0.0033 – – – – – –
Nuclear Periphery 0.0141 0.0045 0.0068 0.002 0.0045 0.0038 0.0108 0.0115 0.0044 0.0028 0.0012 0.023 – – – – – –
Nucleolus 0.0022 0.0006 0.0009 0.0008 0.0001 0.0007 0.0019 0.0037 0.0012 0.0002 0.0001 0.0004 – – – – – –
Nucleus 0.015 0.0027 0.0027 0.0025 0.0005 0.003 0.0086 0.0031 0.0016 0.0011 0.0003 0.0024 – – – – – –
Peroxisomes 0.0148 0.0013 0.0104 0.0026 0.003 0.0038 0.0077 0.0131 0.0084 0.039 0.001 0.0024 – – – – – –
Punctate Nuclear 0.0012 0.0006 0.0006 0.0004 0.0002 0.0003 0.0159 0.0022 0.022 0.001 0.0002 0.0013 – – – – – –
Vacuole 0.1523 0.1812 0.1568 0.2412 0.1581 0.1607 0.1771 0.2126 0.152 0.2169 0.0869 0.2231 – – – – – –
Vacuole Periphery 0.0354 0.0372 0.0233 0.0538 0.0707 0.0826 0.0239 0.0422 0.0543 0.0755 0.023 0.0864 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.171 17.9379 18.1652 22.4392 18.5178 17.9316 24.0583 23.9065 22.2141 25.0891
Translational Efficiency 0.5011 0.4364 0.4161 0.3148 0.3176 0.4021 0.417 0.2861 0.3322 0.3565

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Select an organelle or subcellular structure:


Penetrance Data

Penetrance values (percentage of cells with morphologically abnormal structure) at each time point for each of the subcellular structures monitored.

TP0 TP3 TP6 TP9 TP12 TP15 TP18 TP21 TP24
Autophagosome (ATG8) 21.848739 24.487472 49.667774 69.743234 82.014388 87.887654 88.770053 86.825305 83.208489
Septin (CDC11) 16.288385 16.129032 24.255319 36.589229 54.734177 70.821256 62.589928 57.183365 57.095238
Spindle & SPB (DAD2) 23.076923 34.670487 48.019802 48.378378 63.636364 62.467866 71.309192 79.375000 86.027397
P-bodies (DCP2) 24.315068 40.838852 77.163462 83.333333 89.246120 93.877551 87.708067 84.195933 81.472291
Inner Nuclear Membrane (HEH2) 31.764706 21.538462 29.157175 47.970480 55.614568 62.345229 71.160779 72.509579 74.789128
Metacaspase (MCA1) 8.883827 12.476371 20.508377 36.481069 42.397388 38.482024 35.068604 25.734814 26.763990
Plasma Membrane (MID2) 29.822485 23.317308 27.144608 35.102925 60.343035 72.230147 73.580902 76.089385 80.490588
Necrosis Factor (NHP6A) 23.809524 15.645161 15.310493 35.593220 60.321101 77.102473 76.293103 72.154223 70.790816
Nucleolus (NOP10) 22.318339 40.085592 30.333670 38.652208 60.205479 74.600246 70.785805 66.302953 66.889836
Kinetochore (NUF2) 25.223214 28.431373 13.242961 12.733171 9.635974 21.069383 32.473254 49.612865 48.130564
Mitochondria (OM45) 27.439024 34.577603 35.950413 55.373832 81.854839 95.458937 88.487805 90.451833 92.609489
Eisosomes (PIL1) 19.163763 16.752577 27.158273 48.127601 70.523752 95.263724 98.728814 95.801938 97.267760
Proteasome (PRE2) 19.865772 22.655525 26.381724 32.468354 56.845426 84.778420 81.887755 95.213784 88.415022
DNA Damage Foci (RAD52) 18.421053 21.164773 16.353591 28.012048 60.681629 84.541485 84.361037 79.290508 90.481172
Actin Patches (SAC6) 19.109948 21.073559 38.969617 38.872692 68.830060 91.209677 94.560995 94.170404 94.186047
Golgi, Early (SEC21) 13.986014 17.444934 19.258295 22.571589 31.749242 48.885794 67.002519 74.254090 65.910319
Golgi, Late (SEC7) 32.981530 41.973244 63.398693 77.914110 85.128806 92.419175 85.287846 80.295567 80.468750
Endosomes (SNF7) 38.938053 18.906064 20.688419 25.338172 28.602757 40.714286 37.878305 35.137672 36.258226
Endoplasmic Reticulum (SPF1) 20.275591 17.663818 25.708290 33.627538 42.660944 67.318982 66.639209 64.223798 59.240506
Lipid Droplets (TGL3) 10.020510 13.448489 9.892927 12.638581 14.604868 15.397797 22.110092 22.087746 21.855633
Vacuole (VPH1) 42.476190 25.853659 54.811715 67.573696 81.577363 88.027295 88.598131 90.804598 93.582888

Micrographs

Cell Count

Number of cells analysed for each strain. R1, R2: biological replicates; R1 & R2: both replicates combined.

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
111 1436 729 799 1524 1779 1223 51 1635 3215 1952 850

Protein Abundance

Mean and standard deviation of cell GFP intensity, and intensity change (log2) of each mutant relative to wild type.

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 621.92 725.89 839.23 743.03 618.33 707.75 761.75 749.34 618.57 715.85 790.69 743.41
Standard Deviation 93.84 98.36 104.69 122.72 72.34 97.54 92.03 74.99 74.00 98.32 103.94 120.40
Intensity Change Log 2 — 0.223022 0.432337 0.256691 — 0.194863 0.300941 0.277243 — 0.209052 0.368324 0.266974

Localization Score (DeepLoc)

Localization score for each compartment. Values highlighted in green denote significant localization scores (marked * in the plot).

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000213 0.000104 0.000521 0.000867 0.000090 0.000129 0.000214 0.001257 0.000099 0.000118 0.000329 0.000891
Bud Neck 0.043135 0.012435 0.010189 0.016381 0.012732 0.011573 0.014357 0.008758 0.014796 0.011958 0.012800 0.015923
Bud Site 0.009167 0.014370 0.022196 0.037599 0.010598 0.014886 0.010747 0.028065 0.010501 0.014656 0.015023 0.037027
Cell Periphery 0.000485 0.000116 0.000077 0.000133 0.000136 0.000240 0.000134 0.000330 0.000160 0.000185 0.000112 0.000145
Cytoplasm 0.207425* 0.189572* 0.182816* 0.253976* 0.217728* 0.104360* 0.299297* 0.196302* 0.217029* 0.142420* 0.255796* 0.250516*
Cytoplasmic Foci 0.280659* 0.253090* 0.164656 0.111208 0.273173* 0.187333* 0.116851 0.108181 0.273682* 0.216704* 0.134704 0.111027
Eisosomes 0.000800 0.000057 0.000040 0.000068 0.000100 0.000061 0.000071 0.000359 0.000148 0.000059 0.000059 0.000085
Endoplasmic Reticulum 0.003511 0.003710 0.004018 0.001070 0.009783 0.004495 0.001904 0.002856 0.009357 0.004144 0.002693 0.001177
Endosome 0.113033 0.166591 0.139394 0.073376 0.112734 0.214701* 0.082871 0.094488 0.112755 0.193212 0.103980 0.074643
Golgi 0.035300 0.011855 0.009708 0.006242 0.013049 0.021948 0.001501 0.012688 0.014560 0.017440 0.004566 0.006629
Lipid Particles 0.032949 0.011367 0.004860 0.004224 0.019835 0.011357 0.006299 0.017560 0.020726 0.011361 0.005761 0.005024
Mitochondria 0.074480 0.017121 0.007217 0.013312 0.024419 0.032541 0.002353 0.001340 0.027818 0.025654 0.004170 0.012593
Mitotic Spindle 0.000192 0.002503 0.047341 0.030173 0.001344 0.005885 0.008819 0.000143 0.001266 0.004374 0.023206 0.028371
None 0.000320 0.001654 0.000643 0.000981 0.002232 0.000580 0.001810 0.000651 0.002102 0.001060 0.001374 0.000961
Nuclear Periphery 0.000365 0.000694 0.002842 0.000658 0.001086 0.000919 0.001337 0.001344 0.001037 0.000819 0.001899 0.000699
Nuclear Periphery Foci 0.000123 0.000603 0.006520 0.000853 0.000592 0.000471 0.002607 0.000473 0.000560 0.000530 0.004068 0.000830
Nucleolus 0.002465 0.001952 0.004696 0.000845 0.001819 0.001127 0.004762 0.000777 0.001863 0.001496 0.004738 0.000841
Nucleus 0.051327 0.013641 0.045372 0.023001 0.023123 0.007638 0.118635 0.151802* 0.025038 0.010319 0.091274 0.030730
Peroxisomes 0.012595 0.003257 0.008258 0.005331 0.002917 0.004352 0.004065 0.024320 0.003574 0.003863 0.005631 0.006470
Vacuole 0.108758 0.240617* 0.298103* 0.413068* 0.227064* 0.287333* 0.316606* 0.344054* 0.219032* 0.266467* 0.309696* 0.408928*
Vacuole Periphery 0.022700 0.054691 0.040535 0.006634 0.045444 0.088071 0.004760 0.004253 0.043900 0.073162 0.018121 0.006491

Localization Changes (T-score)

Change in localization of each mutant relative to its control (e.g. UBP2_WT: ubp2Δ vs wild type). Values highlighted in yellow (increase) or blue (decrease) denote significant changes (marked * in the plot).

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.28 -3.31 -2.84 -3.78 -1.13 -1.22 -1.66 -1.42 -1.37 -1.27 -0.93 -4.67 -4.06 -3.93 -2.55
Bud Neck 3.42 3.58 3.13 -1.40 -2.31 0.86 -1.04 2.05 1.53 2.68 2.17 1.23 0.15 -1.71 -0.92
Bud Site -2.09 -4.69 -5.81 -5.24 -3.04 -2.89 -0.04 -1.01 -0.77 -1.01 -3.51 -3.28 -6.33 -5.21 -5.08
Cell Periphery 4.58 5.04 4.51 -0.14 -2.59 -1.13 0.41 -3.27 -0.70 -3.36 -0.48 4.19 1.80 1.04 -1.22
Cytoplasm 0.88 0.97 -2.53 -6.22 -5.58 16.86 -8.50 0.73 -3.01 3.27 11.86 -4.92 -3.66 -11.47 -0.02
Cytoplasmic Foci 1.57 6.81 9.98 22.08 7.63 13.35 24.64 9.47 4.66 0.74 9.86 24.50 26.89 21.20 5.79
Eisosomes 2.93 2.99 2.91 -1.31 -4.90 7.59 5.62 -2.82 -3.28 -3.15 4.88 4.80 3.53 -3.05 -2.84
Endoplasmic Reticulum -0.14 -0.52 1.78 9.81 9.98 4.76 7.47 3.23 0.92 -0.43 5.15 6.61 8.33 11.30 8.17
Endosome -3.80 -1.27 3.68 17.52 9.72 -18.12 5.65 0.92 5.14 -0.35 -17.47 2.50 9.70 25.63 7.10
Golgi 2.98 3.36 3.84 5.10 2.66 -5.55 11.38 0.13 1.20 -1.26 -2.20 9.22 5.97 8.64 -0.92
Lipid Particles 4.48 5.87 6.16 7.29 2.10 5.96 10.76 0.37 -0.58 -1.15 7.44 12.92 12.87 7.32 1.73
Mitochondria 4.14 4.92 4.56 2.22 -1.38 -3.22 13.07 13.04 14.64 1.01 0.98 12.94 5.87 5.65 -2.38
Mitotic Spindle -4.08 -10.51 -7.00 -6.39 2.74 -3.63 -3.90 1.78 5.42 4.76 -3.52 -10.14 -6.65 -5.88 -1.11
None -2.78 -2.73 -2.58 1.34 -1.23 3.11 0.84 2.33 -0.07 2.15 1.99 1.49 2.23 0.42 1.54
Nuclear Periphery -2.01 -5.43 -3.79 -0.41 4.66 0.54 -1.19 -0.32 -0.59 0.15 0.91 -3.49 1.19 0.19 5.81
Nuclear Periphery Foci -6.24 -8.22 -4.66 -1.79 7.23 1.12 -2.78 0.82 0.07 2.93 0.37 -6.80 -1.93 -2.22 6.04
Nucleolus 0.28 -1.27 0.90 2.33 4.53 1.60 -3.14 2.49 1.28 4.52 0.90 -3.99 2.50 2.37 6.06
Nucleus 2.57 0.08 1.87 -4.73 5.91 10.07 -19.19 -4.16 -4.69 -0.91 8.52 -17.87 -1.95 -7.64 14.21
Peroxisomes 4.19 1.82 3.42 -2.42 2.81 -2.22 -2.60 -1.14 -1.06 -1.07 -0.70 -3.88 -1.87 -1.58 -0.16
Vacuole -9.02 -12.45 -18.77 -15.78 -9.39 -7.63 -9.98 -3.21 -1.76 -1.03 -7.28 -12.55 -17.98 -14.44 -9.86
Vacuole Periphery -6.47 -2.68 4.00 18.35 10.14 -12.01 18.30 15.54 26.27* 0.36 -10.37 11.51 17.17 31.71* 7.59
Standard name
Human Ortholog
Description (SGD) Ubiquitin-binding protein; centrally regulates metabolic homeostasis and carbon utilization during nutrient limitation by managing Snf1p activity; subunit of TORC1 rapamycin-sensitive complex involved in growth control; central scaffold that holds the different components of TORC1 together; contains four HEAT repeats and seven WD-40 repeats
Localization
Cell Percentages cytoplasm (30%), vacuolar membrane (18%), mixed (28%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Kog1

Kog1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kog1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available