Standard name
Human Ortholog
Description Putative protein of unknown function; mutant has long chronological lifespan; has homology to Staphylococcus aureus nuclease; GFP-fusion protein localizes to mitochondria; is induced in response to the DNA-damaging agent MMS
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0.06 0 0 0 0.08 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0 0.22 0.19 0.23 0.12 0.15 0.25 0.21 0.08 0.09 0.1 0.11 0.49 0.51 0.56 – 0.28 0.35 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.07 0.12 0 0 0 0 0 0
Endosome 0.27 0.1 0.25 0.05 0 0 0.1 0.12 0 0 0 0 0.19 0.17 0.15 – 0.19 0.09 0.14 0.07 0.05 0.07 0.06 0.07
Golgi 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0
Mitochondria 0.63 0.63 0.44 0.73 0.88 0.82 0.61 0.56 0.93 0.88 0.9 0.88 0.13 0.08 0.12 – 0.11 0.12 0.58 0.73 0.8 0.72 0.71 0.67
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0.1 0.06 0.12 0.05 0 0 0 0 0 0 0 0 0.11 0.15 0.13 – 0.2 0 0 0 0.07 0 0 0
SpindlePole 0 0 0.12 0 0.06 0.07 0.06 0.13 0.08 0.07 0.06 0.06 0.06 0.07 0 – 0.17 0.22 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.1 0.06 0 0 0 0.07 0.07 0 0 0 0 0.08 0.07 0.08 – 0.13 0.14 0.11 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 1 0 0 0 0 1 0 0 1 0 2 4 1 – 0 5 5 3 0 6 12 15
Bud 2 1 0 2 1 0 2 4 11 2 6 5 1 5 0 – 5 4 1 0 0 2 7 7
Bud Neck 0 0 0 0 0 0 0 2 1 0 1 2 1 2 0 – 3 2 2 0 0 1 5 4
Bud Site 1 1 0 0 1 0 0 1 4 1 3 4 0 0 0 – 0 3 – – – – – –
Cell Periphery 2 4 0 2 0 1 0 1 0 1 0 0 9 8 4 – 8 3 0 0 0 1 2 0
Cytoplasm 5 32 3 14 2 8 17 26 12 12 14 16 101 188 207 – 85 84 1 5 0 5 10 15
Endoplasmic Reticulum 0 3 0 1 0 1 1 0 0 2 0 1 8 9 8 – 21 29 3 2 0 2 6 6
Endosome 49 14 4 3 0 0 7 15 0 0 0 0 40 63 57 – 59 21 41 12 3 19 34 30
Golgi 0 0 1 0 0 0 0 1 0 1 0 0 1 3 2 – 6 1 16 5 0 6 16 10
Mitochondria 112 92 7 45 14 45 42 69 135 119 126 126 27 31 44 – 32 28 172 139 44 200 379 303
Nucleus 3 2 0 0 0 1 1 5 1 2 2 3 0 0 1 – 4 8 1 2 0 1 3 1
Nuclear Periphery 2 1 0 1 0 0 0 1 1 1 1 0 0 0 0 – 0 0 4 0 0 1 1 6
Nucleolus 11 0 0 0 0 0 0 0 2 2 0 0 0 1 0 – 0 0 4 0 0 2 1 0
Peroxisomes 18 8 2 3 0 0 0 2 0 1 2 0 23 54 48 – 61 7 4 3 3 7 22 19
SpindlePole 6 3 2 1 1 4 4 16 12 9 8 9 12 25 13 – 53 52 3 3 0 4 8 5
Vac/Vac Membrane 20 15 1 2 0 1 5 9 4 4 2 5 17 24 28 – 40 33 31 7 0 13 18 17
Unique Cell Count 179 145 16 62 16 55 69 124 145 136 140 143 207 369 370 303 238 299 191 56 278 536 455
Labelled Cell Count 231 178 21 74 19 61 79 153 183 157 166 171 242 417 413 377 280 299 191 56 278 536 455


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 4.5 5.6 4.9 3.7 4.4 4.8 5.2 3.7 3.8 3.6 3.7 5.7 5.5 5.5 4.5 5.5 5.4 5.8 6.3 6.9
Std Deviation (1e-4) 0.6 3.0 0.8 1.8 0.5 1.9 1.8 2.3 1.5 1.1 0.9 1.0 1.3 1.4 1.4 1.0 1.5 1.2 1.3 2.0 2.2
Intensity Change (Log2) – – – -0.18 -0.61 -0.35 -0.23 -0.11 -0.61 -0.55 -0.63 -0.58 0.02 -0.03 -0.04 -0.31 -0.01 -0.05 0.06 0.18 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0457 0.0327 0.0325 -0.4428 -0.1891 0.281 5.2094 5.2833 5.313 4.6174 4.6448 5.4299 2.1958 2.5149 2.3835 2.2566 2.0415 2.5638
Actin 0.0428 0.0079 0.0548 0.0084 0.0124 0.01 0.025 0.0167 0.0257 0.0094 0.0339 0.0178 0.0192 0.006 0.0131 0.0297 0.0078 0.009
Bud 0.0028 0.0024 0.0038 0.0048 0.0019 0.0019 0.0016 0.0047 0.004 0.0022 0.0063 0.0016 0.002 0.0047 0.0032 0.0027 0.0104 0.0018
Bud Neck 0.0051 0.0013 0.0038 0.0012 0.0019 0.0144 0.0018 0.0031 0.0068 0.0019 0.0044 0.0091 0.0139 0.0058 0.0073 0.0019 0.0014 0.0102
Bud Periphery 0.0071 0.0053 0.0098 0.0212 0.0061 0.0065 0.0039 0.0076 0.0121 0.0069 0.0224 0.0045 0.0046 0.0098 0.0079 0.0059 0.0526 0.0045
Bud Site 0.0171 0.0255 0.0258 0.0043 0.0017 0.0015 0.0064 0.0544 0.0249 0.0079 0.0072 0.0021 0.0116 0.0593 0.0186 0.0073 0.0029 0.0024
Cell Periphery 0.0025 0.0018 0.0033 0.0012 0.0008 0.0012 0.0025 0.0026 0.0049 0.0013 0.0018 0.0011 0.0018 0.0027 0.0026 0.0007 0.0012 0.0018
Cytoplasm 0.0657 0.1236 0.0773 0.0718 0.0798 0.0888 0.0601 0.0864 0.0943 0.0647 0.0945 0.0852 0.0824 0.0891 0.0662 0.0959 0.0899 0.0754
Cytoplasmic Foci 0.1082 0.1185 0.0971 0.1256 0.1706 0.1312 0.1719 0.1443 0.1254 0.1419 0.1516 0.1737 0.1523 0.1254 0.1254 0.1477 0.15 0.1457
Eisosomes 0.0023 0.0007 0.0026 0.0006 0.0017 0.0004 0.0019 0.0007 0.0016 0.0007 0.0042 0.0012 0.0012 0.0006 0.002 0.0005 0.0003 0.0009
Endoplasmic Reticulum 0.0098 0.0146 0.0062 0.0025 0.0025 0.0085 0.0049 0.0027 0.0047 0.0027 0.0046 0.0031 0.0072 0.0056 0.0066 0.0044 0.0031 0.0039
Endosome 0.1587 0.1717 0.1133 0.081 0.1795 0.1282 0.1528 0.1582 0.1317 0.1653 0.2591 0.1258 0.1746 0.1679 0.0931 0.1701 0.1383 0.1106
Golgi 0.0472 0.0476 0.0432 0.038 0.0592 0.0469 0.0553 0.0783 0.048 0.0662 0.1069 0.0524 0.0466 0.0529 0.05 0.0567 0.0385 0.0363
Lipid Particles 0.0603 0.0188 0.0232 0.0105 0.0379 0.0398 0.0347 0.0187 0.0194 0.0049 0.0059 0.0156 0.0444 0.0154 0.0164 0.0053 0.0234 0.0327
Mitochondria 0.1822 0.2289 0.2082 0.331 0.2829 0.2568 0.239 0.2361 0.3035 0.3002 0.1797 0.2908 0.1627 0.1586 0.3128 0.2769 0.2186 0.2685
None 0.2121 0.1795 0.2618 0.2058 0.0615 0.1659 0.1361 0.1302 0.1358 0.1542 0.0552 0.1362 0.2226 0.2389 0.2177 0.1506 0.193 0.2168
Nuclear Periphery 0.009 0.003 0.0031 0.0007 0.0065 0.0145 0.0172 0.0008 0.001 0.0007 0.0036 0.0009 0.0021 0.002 0.0074 0.0046 0.0008 0.0016
Nucleolus 0.0031 0.0013 0.0015 0.001 0.001 0.0008 0.001 0.0008 0.0015 0.0006 0.0004 0.0008 0.0025 0.0016 0.0015 0.001 0.0007 0.0014
Nucleus 0.0054 0.006 0.0021 0.0013 0.0025 0.0069 0.0142 0.0011 0.0016 0.0008 0.0016 0.001 0.0023 0.0025 0.0033 0.0017 0.001 0.0074
Peroxisomes 0.0324 0.0171 0.0371 0.0744 0.0412 0.06 0.0426 0.041 0.0366 0.0575 0.0387 0.065 0.0267 0.0278 0.0295 0.0212 0.0516 0.0521
Punctate Nuclear 0.0033 0.0017 0.0027 0.0019 0.0274 0.0027 0.0165 0.0009 0.0019 0.0006 0.0036 0.0015 0.0032 0.0019 0.0053 0.0017 0.0028 0.0045
Vacuole 0.0165 0.0176 0.0142 0.0074 0.0159 0.0085 0.0074 0.0078 0.0104 0.0056 0.0092 0.0073 0.012 0.016 0.0074 0.008 0.0081 0.0086
Vacuole Periphery 0.0062 0.0052 0.0051 0.0054 0.0051 0.0047 0.0034 0.0029 0.0038 0.0039 0.0051 0.0033 0.0039 0.0054 0.0026 0.0055 0.0037 0.0036

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.356 8.3693 3.5488 8.5914 5.2022 9.357 8.7644 12.6466 10.0086 8.3905
Translational Efficiency 0.73 0.5308 1.1009 0.6471 0.8587 0.5943 0.647 0.3834 0.4615 0.552

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
41 1200 2638 1749 1395 2418 2418 1653 1436 3618 5056 3402

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 687.61 664.43 806.69 784.63 581.13 816.87 753.37 774.92 584.17 766.31 781.19 779.91
Standard Deviation 91.58 110.13 95.28 130.87 78.84 93.79 109.28 106.65 81.20 122.69 105.63 119.82
Intensity Change Log 2 — -0.049473 0.230424 0.190422 — 0.491246 0.374498 0.415186 — 0.223467 0.298205 0.297733

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002799 0.002043 0.006498 0.006171 0.000657 0.002231 0.002406 0.010260 0.000718 0.002169 0.004541 0.008158
Bud Neck 0.004003 0.015074 0.051836 0.041023 0.028703 0.017514 0.069109 0.035630 0.027998 0.016705 0.060097 0.038402
Bud Site 0.019221 0.022689 0.083841 0.069182 0.020320 0.018413 0.048450 0.097184 0.020289 0.019831 0.066916 0.082788
Cell Periphery 0.000936 0.000492 0.000211 0.000237 0.000360 0.000228 0.000397 0.000339 0.000376 0.000316 0.000300 0.000287
Cytoplasm 0.023431 0.018422 0.138110* 0.212701* 0.040195 0.026939 0.154513* 0.158669* 0.039717 0.024114 0.145955* 0.186447*
Cytoplasmic Foci 0.092008 0.097116 0.114131 0.122839 0.146823 0.119920 0.119035 0.177653 0.145258 0.112357 0.116477 0.149473
Eisosomes 0.000325 0.000155 0.000111 0.000144 0.000165 0.000218 0.000210 0.000232 0.000170 0.000197 0.000158 0.000186
Endoplasmic Reticulum 0.000412 0.000894 0.004523 0.005194 0.001170 0.000528 0.003501 0.003341 0.001149 0.000649 0.004034 0.004294
Endosome 0.034944 0.105855 0.040770 0.042613 0.086949 0.069975 0.058770 0.054549 0.085464 0.081876 0.049378 0.048413
Golgi 0.188874* 0.142025* 0.021704 0.014402 0.092687 0.126946* 0.014206 0.043743 0.095433 0.131947* 0.018118 0.028658
Lipid Particles 0.008075 0.006331 0.003994 0.006523 0.018437 0.006805 0.019375 0.008281 0.018141 0.006648 0.011350 0.007377
Mitochondria 0.478300* 0.472142* 0.139584* 0.045240 0.447551* 0.508099* 0.084493 0.097022 0.448429* 0.496173* 0.113237* 0.070400
Mitotic Spindle 0.008273 0.014438 0.123100* 0.061288 0.009395 0.011542 0.049027 0.049945 0.009363 0.012503 0.087675 0.055776
None 0.010027 0.002073 0.000840 0.003211 0.002905 0.000536 0.003680 0.001966 0.003108 0.001046 0.002199 0.002606
Nuclear Periphery 0.000077 0.000123 0.002155 0.001892 0.000671 0.000073 0.002763 0.000733 0.000654 0.000090 0.002446 0.001329
Nuclear Periphery Foci 0.000227 0.000270 0.000908 0.002152 0.000518 0.000098 0.001293 0.001137 0.000510 0.000155 0.001092 0.001659
Nucleolus 0.006382 0.001936 0.002196 0.003368 0.004149 0.000566 0.008384 0.001734 0.004213 0.001020 0.005155 0.002574
Nucleus 0.007293 0.004239 0.058553 0.118842 0.013126 0.003914 0.144834* 0.036250 0.012959 0.004022 0.099817 0.078711
Peroxisomes 0.097552 0.060684 0.058832 0.047174 0.042030 0.056235 0.048944 0.088586 0.043615 0.057711 0.054103 0.067296
Vacuole 0.011073 0.019857 0.140239 0.187717 0.025423 0.019889 0.157642 0.124824 0.025013 0.019878 0.148562 0.157158
Vacuole Periphery 0.005770 0.013143 0.007863 0.008086 0.017764 0.009329 0.008968 0.007922 0.017422 0.010594 0.008392 0.008007

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.71 -3.11 -1.94 -3.40 0.96 -4.36 -6.58 -8.98 -7.04 -7.08 -4.91 -11.49 -10.16 -7.43 -4.21
Bud Neck -5.75 -23.49 -14.71 -9.32 5.94 4.35 -11.30 -2.13 -7.74 10.20 4.72 -12.13 -3.37 -11.88 11.57
Bud Site -0.34 -5.49 -3.61 -9.80 4.86 0.91 -9.71 -17.73 -19.03 -10.76 0.19 -17.78 -18.71 -21.03 -3.90
Cell Periphery 1.13 1.90 1.87 4.08 -0.38 2.74 -0.10 0.83 -3.24 1.19 1.16 1.86 2.35 1.88 0.46
Cytoplasm 0.40 -9.95 -15.09 -32.33 -10.88 4.08 -22.70 -20.66 -24.90 -1.07 5.09 -27.86 -31.31 -40.27 -8.66
Cytoplasmic Foci -0.35 -1.08 -1.09 -2.54 -0.10 5.18 5.46 -3.87 -10.14 -10.36 6.82 6.75 1.23 -7.63 -7.67
Eisosomes 1.50 1.91 1.66 1.47 -4.42 -2.41 -3.17 -3.33 -0.14 -1.16 -1.66 1.70 -0.75 1.16 -2.76
Endoplasmic Reticulum -2.00 -14.65 -14.29 -11.40 -0.80 2.79 -8.07 -5.86 -8.59 0.77 2.21 -11.53 -10.50 -15.09 -0.04
Endosome -5.66 -0.25 -0.00 15.00 1.36 3.92 7.72 8.68 5.96 1.22 0.86 10.60 11.39 15.25 1.73
Golgi 1.21 4.43 4.65 24.50 5.83 -6.52 19.68 11.77 21.10 -11.08 -7.39 19.46 16.88 33.85 -6.12
Lipid Particles 0.49 0.95 0.52 0.12 -4.01 6.79 0.08 5.79 -1.37 6.52 7.07 4.32 6.85 -0.81 4.78
Mitochondria 0.12 6.36 8.02 42.24* 19.94* -5.39 37.41* 34.84* 51.61* -2.01 -4.55 36.12 40.62* 70.12* 11.63
Mitotic Spindle -1.71 -21.42* -9.89 -10.61 11.25 -1.37 -13.26 -11.97 -11.31 -0.14 -2.24 -27.89 -16.49 -15.67 9.04
None 1.15 1.31 1.04 -1.10 -4.35 5.60 -0.73 1.82 -2.79 2.31 4.17 2.07 1.38 -3.41 -0.74
Nuclear Periphery -0.79 -13.95 -11.77 -11.89 0.65 2.38 -5.30 -0.55 -5.36 5.63 2.31 -6.68 -3.49 -12.81 5.01
Nuclear Periphery Foci -0.22 -4.29 -5.10 -5.33 -3.13 4.55 -4.79 -3.32 -5.74 0.55 3.92 -5.23 -5.42 -7.52 -2.38
Nucleolus 0.93 0.88 0.66 -1.93 -2.24 5.24 -4.78 3.35 -4.80 10.18 4.54 -1.49 2.21 -4.80 6.59
Nucleus 0.76 -12.06 -20.42 -26.77 -12.92 5.48 -28.62 -9.79 -15.36 21.39 5.55 -30.65 -23.81 -29.53 3.38
Peroxisomes 1.15 1.31 1.83 4.60 4.84 -3.85 -1.35 -8.81 -5.83 -8.15 -4.06 -2.49 -4.81 -0.96 -3.25
Vacuole -1.27 -18.29 -22.84 -34.03 -9.05 2.58 -31.27 -24.00 -25.87 3.18 2.53 -42.16 -38.53 -42.35 -4.52
Vacuole Periphery -3.13 -0.61 -0.17 6.36 1.32 7.27 8.38 9.05 2.90 1.25 6.14 9.30 9.81 6.39 1.81
Standard name
Human Ortholog
Description Putative protein of unknown function; mutant has long chronological lifespan; has homology to Staphylococcus aureus nuclease; GFP-fusion protein localizes to mitochondria; is induced in response to the DNA-damaging agent MMS
Localization
Cell Percentages mitochondrion (86%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Lcl3

Lcl3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lcl3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available