Standard name
Human Ortholog
Description Mitochondrial protein of unknown function involved in lipid homeostasis; integral membrane protein that localizes to the mitochondrial outer membrane; involved in mitochondrial morphology; interacts genetically with MDM10, and other members of the ERMES complex; contains five predicted transmembrane domains
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0.05 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0.05 0.05 0 0 0 – 0 – 0 0 0 0 0 0
Bud Neck 0 0 0.07 0 0 0.05 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Cytoplasm 0.83 0.81 0.83 0.77 0.51 0.33 0.49 – 0.33 0.35 0.28 0.34 0.9 0.95 0.96 – 0.69 – 0.36 0.37 0.18 0.33 0.34 0.26
Endoplasmic Reticulum 0 0 0 0 0 0.08 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Endosome 0 0 0 0 0.08 0.08 0 – 0 0 0 0 0 0 0 – 0.08 – 0 0 0.05 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Mitochondria 0.12 0.19 0 0.07 0.11 0.39 0.52 – 0.74 0.67 0.69 0.69 0.05 0 0 – 0.07 – 0.37 0.41 0.57 0.39 0.43 0.43
Nucleus 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
SpindlePole 0 0 0.07 0.06 0.16 0.1 0.07 – 0 0.07 0.09 0.07 0 0 0 – 0.06 – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.09 0 0 – 0 0 0.05 0 0 0 0 – 0.1 – 0.07 0.09 0 0.07 0.07 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 2 4 7 4 3 3 – 0 0 1 1 0 0 0 – 0 – 6 7 9 3 5 3
Bud 2 5 2 5 5 4 6 – 11 7 12 19 1 3 0 – 1 – 8 2 3 0 2 2
Bud Neck 2 5 11 6 6 10 9 – 3 3 4 4 0 0 2 – 0 – 3 5 5 4 3 4
Bud Site 0 0 0 0 0 0 3 – 6 2 3 4 0 0 0 – 0 – – – – – – –
Cell Periphery 1 1 2 7 5 1 0 – 0 1 1 1 3 1 0 – 2 – 2 3 1 1 2 2
Cytoplasm 166 229 127 129 122 71 135 – 84 78 73 136 137 226 220 – 74 – 88 123 34 49 73 48
Endoplasmic Reticulum 6 2 1 2 4 18 3 – 1 0 7 2 2 0 0 – 3 – 4 2 3 0 1 1
Endosome 1 1 3 3 20 17 1 – 1 1 6 3 1 3 3 – 9 – 9 11 9 6 6 7
Golgi 1 0 0 0 1 0 0 – 0 1 0 0 1 0 0 – 0 – 2 0 0 1 1 0
Mitochondria 25 54 1 11 26 83 143 – 187 147 181 277 7 3 0 – 8 – 89 138 110 57 93 80
Nucleus 1 1 0 0 3 10 9 – 3 3 0 8 1 0 2 – 2 – 0 1 0 0 0 2
Nuclear Periphery 0 0 0 0 1 2 0 – 1 0 0 0 0 0 0 – 0 – 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 1 – 1 0 0 0 0 0 0 – 0 – 0 0 0 0 0 1
Peroxisomes 0 1 0 0 8 1 0 – 0 0 5 0 0 2 2 – 2 – 0 0 2 0 0 0
SpindlePole 0 1 10 10 37 22 20 – 11 16 24 29 3 1 2 – 6 – 5 4 4 6 6 2
Vac/Vac Membrane 4 6 4 0 21 4 1 – 3 5 12 5 1 3 3 – 11 – 17 29 3 9 14 17
Unique Cell Count 200 283 153 168 237 212 275 254 220 261 399 153 237 228 107 243 338 195 149 216 187
Labelled Cell Count 210 308 165 180 263 246 334 312 264 329 489 157 242 234 118 243 338 195 149 216 187


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 3.6 5.1 4.9 5.2 4.5 3.2 – 3.5 3.8 3.7 3.8 5.0 4.9 4.8 4.2 5.2 4.9 5.2 5.4 5.5
Std Deviation (1e-4) 0.7 0.6 1.0 1.2 1.2 1.7 1.0 – 1.2 1.0 1.1 1.3 1.2 1.4 1.3 1.2 1.6 1.0 1.2 1.3 1.7
Intensity Change (Log2) – – – -0.07 0.03 -0.2 -0.67 – -0.55 -0.42 -0.49 -0.42 -0.05 -0.06 -0.1 -0.28 0.03 -0.08 0.02 0.08 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 –
Bud Neck – – – – – – – – – – – – – 0 –
Bud Site – – – – – – – – – – – – – 0 –
Cell Periphery – – – – – – – – – – – – – 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 –
Endosome – – – – – – – – – – – – – 0 –
Golgi – – – – – – – – – – – – – 0 –
Mitochondria – – – – – – – – – – – – – 0 –
Nuclear Periphery – – – – – – – – – – – – – 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 –
Peroxisomes – – – – – – – – – – – – – 0 –
SpindlePole – – – – – – – – – – – – – 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 –
Cytoplasm – – – – – – – – – – – – – 0 –
Nucleus – – – – – – – – – – – – – 0 –
Vacuole – – – – – – – – – – – – – 2.6 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.0255 4.0401 4.2505 3.8613 3.4252 3.9533 4.1955 4.0736 3.4813 3.092 3.7729 3.3765 1.1184 1.5995 1.2876 1.4148 1.4203 1.2566
Actin 0.1088 0.0061 0.0311 0.0465 0.0236 0.0215 0.0449 0.0046 0.012 0.0184 0.0136 0.0071 0.0681 0.0031 0.0012 0.024 0.0329 0.0073
Bud 0.005 0.0012 0.0014 0.0015 0.0042 0.0016 0.0032 0.0042 0.0082 0.0026 0.0133 0.0036 0.0008 0.0056 0.0009 0.0014 0.0011 0.0009
Bud Neck 0.0103 0.0009 0.002 0.001 0.0021 0.0098 0.0081 0.0012 0.0085 0.0813 0.0022 0.0095 0.0055 0.0013 0.0014 0.0011 0.0037 0.0145
Bud Periphery 0.0045 0.0021 0.0029 0.004 0.0159 0.0077 0.007 0.009 0.0115 0.0105 0.0273 0.0078 0.0016 0.0064 0.0026 0.011 0.0026 0.0024
Bud Site 0.0241 0.0302 0.0216 0.0026 0.0022 0.0008 0.0162 0.0513 0.0226 0.0027 0.0206 0.0015 0.0038 0.0472 0.0042 0.0013 0.0022 0.0013
Cell Periphery 0.0019 0.0024 0.0024 0.0014 0.0035 0.0008 0.0027 0.0043 0.0037 0.0012 0.001 0.0034 0.0008 0.0027 0.0022 0.0026 0.0007 0.0016
Cytoplasm 0.117 0.3599 0.2853 0.2752 0.3489 0.3409 0.1582 0.2501 0.2292 0.1698 0.2477 0.2712 0.0939 0.2922 0.2847 0.2617 0.286 0.3741
Cytoplasmic Foci 0.0714 0.069 0.0509 0.0371 0.0688 0.0776 0.0964 0.0599 0.0606 0.0503 0.0864 0.0627 0.1117 0.0475 0.0422 0.0759 0.0583 0.0768
Eisosomes 0.0016 0.0004 0.0009 0.0014 0.0007 0.0003 0.0011 0.0016 0.0007 0.0007 0.0004 0.0004 0.0011 0.0005 0.0008 0.001 0.0005 0.0003
Endoplasmic Reticulum 0.0248 0.0087 0.0045 0.0042 0.0057 0.0029 0.0106 0.0067 0.0079 0.0113 0.0021 0.0038 0.0064 0.0059 0.0054 0.0118 0.0095 0.0049
Endosome 0.0474 0.0403 0.0467 0.0326 0.0791 0.0421 0.0603 0.0294 0.0464 0.038 0.0347 0.0416 0.089 0.0396 0.0295 0.0436 0.1063 0.0445
Golgi 0.0196 0.0073 0.011 0.0188 0.0701 0.0155 0.0132 0.0046 0.0074 0.0028 0.0288 0.0135 0.0146 0.0073 0.0044 0.0102 0.0117 0.0132
Lipid Particles 0.032 0.0045 0.0132 0.0035 0.0091 0.0071 0.0228 0.0038 0.0077 0.0118 0.0183 0.008 0.0261 0.0042 0.002 0.0026 0.0349 0.007
Mitochondria 0.0185 0.0075 0.017 0.0471 0.0447 0.0186 0.0313 0.0183 0.0291 0.0138 0.0546 0.0417 0.0332 0.0282 0.0209 0.0621 0.037 0.022
None 0.4275 0.4369 0.4759 0.5045 0.3008 0.4301 0.4299 0.5372 0.5199 0.5287 0.4084 0.5019 0.4944 0.488 0.5877 0.4647 0.3415 0.4126
Nuclear Periphery 0.0093 0.0014 0.0013 0.001 0.0015 0.0004 0.0224 0.0014 0.0017 0.0081 0.001 0.0006 0.0149 0.0014 0.0006 0.0071 0.0062 0.001
Nucleolus 0.0031 0.0006 0.0005 0.0003 0.0003 0.0002 0.002 0.0007 0.0008 0.0008 0.0015 0.0004 0.0008 0.0009 0.0003 0.0005 0.0037 0.0004
Nucleus 0.0113 0.0015 0.0015 0.0009 0.001 0.0006 0.0137 0.0015 0.002 0.0018 0.0018 0.001 0.0032 0.0029 0.0007 0.0014 0.0041 0.001
Peroxisomes 0.0259 0.0076 0.0145 0.0068 0.0054 0.0161 0.0228 0.0018 0.005 0.0217 0.0115 0.014 0.0135 0.0028 0.0018 0.0059 0.0207 0.0049
Punctate Nuclear 0.0241 0.001 0.0042 0.0006 0.0008 0.0018 0.0115 0.003 0.0011 0.01 0.0204 0.0009 0.0046 0.0012 0.0005 0.0008 0.0146 0.0008
Vacuole 0.0096 0.0092 0.0094 0.007 0.0087 0.0026 0.0181 0.0047 0.0116 0.0111 0.0035 0.004 0.0084 0.0098 0.0049 0.0074 0.0157 0.0067
Vacuole Periphery 0.0023 0.0014 0.002 0.0019 0.0027 0.0008 0.0037 0.0008 0.0022 0.0026 0.0009 0.0014 0.0032 0.0013 0.0011 0.0019 0.0061 0.0018

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.0347 5.4256 13.6885 9.3242 3.1477 3.6396 4.1144 10.3672 11.7895 5.6146
Translational Efficiency 0.8423 0.7586 0.4142 1.0578 1.0402 1.0584 1.3713 0.3792 0.6319 0.746

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1771 766 2186 2308 1669 2085 2746 1737 3440 2851 4932 4045

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 619.79 774.34 804.85 847.15 582.31 775.65 805.84 794.76 601.61 775.30 805.40 824.65
Standard Deviation 113.18 110.37 92.45 219.29 65.94 84.90 90.71 95.74 95.16 92.44 91.49 179.02
Intensity Change Log 2 — 0.321188 0.376940 0.450838 — 0.413618 0.468706 0.448732 — 0.366702 0.422122 0.449818

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000305 0.000990 0.000585 0.002362 0.000279 0.001332 0.000327 0.000813 0.000292 0.001241 0.000442 0.001697
Bud Neck 0.054707 0.054625 0.023156 0.039250 0.039653 0.081288 0.021706 0.028222 0.047404 0.074124 0.022348 0.034514
Bud Site 0.008792 0.041664 0.019775 0.073423 0.010072 0.030866 0.018671 0.036119 0.009413 0.033767 0.019160 0.057404
Cell Periphery 0.001330 0.000313 0.000150 0.000213 0.000175 0.000282 0.000116 0.000329 0.000770 0.000291 0.000131 0.000263
Cytoplasm 0.246150* 0.191657* 0.395762* 0.400732* 0.313656* 0.206810* 0.496269* 0.339710* 0.278902* 0.202738* 0.451721* 0.374528*
Cytoplasmic Foci 0.244429* 0.333681* 0.101198 0.063968 0.334199* 0.326094* 0.062014 0.101963 0.287983* 0.328132* 0.079382 0.080283
Eisosomes 0.002306 0.000243 0.000072 0.000111 0.000273 0.000433 0.000053 0.000129 0.001320 0.000382 0.000062 0.000119
Endoplasmic Reticulum 0.007928 0.001985 0.003876 0.002759 0.002110 0.000912 0.001452 0.003461 0.005106 0.001200 0.002526 0.003060
Endosome 0.045331 0.073470 0.014751 0.010813 0.027266 0.050280 0.007273 0.027261 0.036566 0.056511 0.010587 0.017876
Golgi 0.011657 0.028021 0.001592 0.003075 0.010735 0.026593 0.000863 0.002649 0.011210 0.026977 0.001186 0.002892
Lipid Particles 0.060853 0.012047 0.005245 0.002638 0.026850 0.014633 0.002407 0.010020 0.044356 0.013938 0.003665 0.005808
Mitochondria 0.034336 0.056586 0.006396 0.009844 0.026969 0.085644 0.003001 0.010492 0.030762 0.077837 0.004506 0.010122
Mitotic Spindle 0.000503 0.005638 0.017391 0.055652 0.001174 0.005210 0.012327 0.018947 0.000829 0.005325 0.014572 0.039890
None 0.026260 0.002784 0.003166 0.001616 0.003559 0.001353 0.002675 0.004823 0.015246 0.001738 0.002893 0.002993
Nuclear Periphery 0.001687 0.000410 0.003265 0.001374 0.000837 0.000294 0.001584 0.002580 0.001275 0.000325 0.002329 0.001892
Nuclear Periphery Foci 0.001212 0.000389 0.001178 0.000611 0.000450 0.000179 0.000524 0.000942 0.000842 0.000236 0.000814 0.000753
Nucleolus 0.003701 0.001606 0.003298 0.001028 0.002432 0.001317 0.001818 0.003306 0.003085 0.001395 0.002474 0.002006
Nucleus 0.131611* 0.044531 0.294355* 0.132538* 0.127543 0.046032 0.270211* 0.191276* 0.129637 0.045629 0.280912* 0.157761*
Peroxisomes 0.019781 0.048987 0.009918 0.012653 0.016834 0.052610 0.004438 0.009141 0.018351 0.051637 0.006867 0.011145
Vacuole 0.092609 0.091691 0.093165 0.181473 0.051737 0.062344 0.091482 0.202315* 0.072779 0.070229 0.092228 0.190423
Vacuole Periphery 0.004510 0.008682 0.001705 0.003867 0.003198 0.005492 0.000790 0.005502 0.003874 0.006349 0.001195 0.004569

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.04 -4.94 -8.43 -3.94 -6.59 -5.39 -1.35 -2.87 2.14 -2.49 -6.40 -4.46 -8.55 -0.80 -7.11
Bud Neck 0.09 10.89 6.52 4.60 -5.27 -10.92 6.98 4.64 15.59 -2.48 -8.83 12.92 7.69 15.33 -5.99
Bud Site -8.67 -5.68 -15.24 -2.36 -10.41 -11.00 -3.91 -8.98 -0.72 -5.97 -14.51 -6.79 -17.55 -4.19 -12.22
Cell Periphery 6.96 8.31 8.19 4.17 -1.27 -4.93 6.05 -1.80 -0.13 -2.85 6.32 8.70 6.96 2.16 -3.29
Cytoplasm 5.60 -16.73 -16.59 -18.71 0.47 13.37 -18.66 -2.06 -14.93 15.60 12.80 -25.91 -13.09 -25.21 11.88
Cytoplasmic Foci -9.06 23.91 34.21 33.17 12.40 1.15 45.39 35.86 36.96 -9.91 -6.65 48.76 49.63 53.57 1.60
Eisosomes 4.85 5.24 5.19 8.91 -3.49 -4.87 15.47 9.99 10.23 -12.58 4.24 5.71 5.51 12.10 -10.52
Endoplasmic Reticulum 5.82 3.85 5.36 -1.71 3.74 2.39 0.76 -2.20 -7.03 -4.83 6.98 4.13 3.62 -8.00 -1.03
Endosome -5.88 13.77 17.27 15.19 6.62 -9.42 13.62 1.31 10.61 -12.21 -8.80 19.35 14.64 20.91 -6.08
Golgi -5.90 12.58 11.46 9.87 -1.27 -10.09 11.14 8.98 18.21 -4.52 -11.88 16.83 14.44 20.72 -4.16
Lipid Particles 17.25 21.45 22.53 7.95 9.37 6.96 16.31 10.71 4.75 -10.03 17.79 26.33 24.75 10.58 -5.58
Mitochondria -4.00 12.96 12.21 9.71 -2.00 -15.01 13.36 8.80 21.22 -6.78 -14.65 18.64 15.13 23.71 -7.14
Mitotic Spindle -4.01 -10.02 -15.38 -12.47 -7.89 -5.63 -8.25 -8.39 -6.18 -2.63 -7.28 -12.99 -17.40 -14.33 -8.65
None 8.00 8.18 8.76 1.32 3.81 4.47 2.04 -0.65 -3.16 -1.84 9.02 8.37 8.18 -2.19 0.07
Nuclear Periphery 5.01 -4.99 -1.73 -11.17 4.36 5.53 -10.41 -5.86 -7.81 -2.10 6.87 -7.60 -5.53 -13.13 1.55
Nuclear Periphery Foci 4.53 -0.50 3.07 -3.97 5.86 5.08 -1.50 -5.22 -9.31 -4.19 6.49 -0.50 0.44 -11.40 1.42
Nucleolus 5.07 0.08 6.44 1.77 7.32 4.25 1.32 -1.82 -5.89 -2.91 6.83 1.21 4.01 -3.90 2.66
Nucleus 15.69 -23.45 -4.15 -20.92 20.66 18.16 -25.73 -9.97 -25.68 12.84 24.52 -34.73 -9.80 -34.19 24.58
Peroxisomes -8.05 7.20 7.21 11.20 0.53 -14.87 10.85 6.30 19.22 -4.48 -16.73 13.03 9.47 22.25 -3.16
Vacuole -0.10 -3.09 -23.95 -21.02 -22.38 -3.36 -16.44 -28.04 -25.88 -17.04 0.65 -12.17 -36.85 -37.12 -28.06
Vacuole Periphery -4.57 8.25 4.25 6.82 -2.26 -5.22 11.05 -2.73 1.24 -7.80 -5.97 13.14 1.27 6.24 -7.57
Standard name
Human Ortholog
Description Mitochondrial protein of unknown function involved in lipid homeostasis; integral membrane protein that localizes to the mitochondrial outer membrane; involved in mitochondrial morphology; interacts genetically with MDM10, and other members of the ERMES complex; contains five predicted transmembrane domains
Localization
Cell Percentages mitochondrion (11%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Mcp1

Mcp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mcp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available