Standard name
Human Ortholog
Description Peroxisomal malate dehydrogenase; catalyzes interconversion of malate and oxaloacetate; involved in the glyoxylate cycle
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0.08 0.09 0 0 0 0 0 0
Cytoplasm 1.0 1.0 – 0.97 0.97 0.93 0.86 0.85 0.81 0.74 0.8 0.79 1.0 0.99 0.99 0.97 0.96 0.97 0.96 0.97 0.88 0.96 0.96 0.95
Endoplasmic Reticulum 0 0 – 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 – 0 0 0 0.06 0 0.13 0.08 0.19 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0 0 0.09 0.06 0.08 0.18 0.05 0.09 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 3 1 0 2 4 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 2 0 0 1 1 1 5 4 4 1 0 0 0 0 1 0 0 0 0 1 4
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 – 3 7 4 5 11 2 3 5 12 4 8 6 22 18 30 0 0 0 0 0 0
Cytoplasm 217 17 – 221 317 269 384 357 135 231 167 192 290 320 387 398 220 311 209 49 43 180 308 429
Endoplasmic Reticulum 1 0 – 1 3 4 9 22 3 10 2 5 1 3 1 14 11 15 1 0 0 0 0 3
Endosome 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 2
Golgi 0 0 – 1 0 0 0 0 0 0 0 0 0 1 1 0 2 1 0 0 0 0 0 1
Mitochondria 0 0 – 1 1 9 25 8 22 24 40 17 0 0 0 0 0 1 2 0 0 0 1 0
Nucleus 0 0 – 4 2 0 7 9 2 6 5 5 1 1 0 1 0 0 0 0 1 1 1 1
Nuclear Periphery 0 0 – 0 0 0 1 1 1 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 0 0 – 4 5 12 41 24 14 55 10 22 0 0 1 15 6 2 0 0 1 0 1 3
Unique Cell Count 218 17 227 326 288 446 418 167 314 209 243 291 322 389 411 230 320 218 51 49 188 321 452
Labelled Cell Count 220 17 237 335 298 473 439 182 334 235 262 297 333 396 450 258 361 218 51 49 188 321 452


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.0 12.4 – 11.1 11.1 9.8 9.1 9.9 8.1 8.6 8.4 8.9 14.0 15.3 15.2 25.6 26.0 25.7 12.9 13.0 13.9
Std Deviation (1e-4) 1.7 2.4 – 2.6 1.6 1.5 1.5 1.6 1.6 1.9 1.9 1.9 2.1 2.3 2.2 5.6 5.2 5.1 4.4 3.0 2.9
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.382 10.9042 9.7036 8.708 7.3384 9.6911 7.8765 11.2161 10.1759 9.279 8.9413 9.4162 6.6293 8.4637 7.0656 7.3309 5.8984 7.824
Actin 0.0344 0.0007 0.0086 0.0018 0.0411 0.0033 0.0319 0.0002 0.0064 0.0094 0.0149 0.0001 0.0201 0.0006 0.0457 0.0057 0.0104 0.0001
Bud 0.0009 0.0001 0.0052 0.0002 0.0008 0.0002 0.0005 0.0001 0.0002 0.0001 0.0002 0 0.0004 0.0001 0.0014 0.0002 0.0007 0
Bud Neck 0.0008 0.0001 0.0006 0.0006 0.0014 0.0014 0.0014 0.0001 0.0003 0.0006 0.0032 0.0008 0.0005 0.0001 0.0008 0.0005 0.0005 0.0005
Bud Periphery 0.0015 0 0.0024 0.0001 0.0006 0.0002 0.0008 0 0.0002 0 0.0004 0 0.0003 0 0.0037 0 0.0007 0
Bud Site 0.0049 0.0003 0.0025 0.0012 0.002 0.0002 0.0014 0.0002 0.0002 0.0002 0.001 0 0.0008 0.0001 0.0058 0.0002 0.0021 0
Cell Periphery 0.0009 0 0.0001 0 0.0001 0 0.0003 0 0 0 0.0003 0 0.0001 0 0.0006 0 0.0001 0
Cytoplasm 0.7709 0.9638 0.9189 0.8562 0.724 0.9267 0.8516 0.9904 0.9858 0.9545 0.9024 0.9892 0.8934 0.9926 0.8519 0.9197 0.7897 0.9912
Cytoplasmic Foci 0.0224 0.0044 0.0116 0.0598 0.0314 0.0154 0.0154 0.0027 0.0012 0.0046 0.0022 0.0026 0.009 0.0019 0.0152 0.0319 0.035 0.0029
Eisosomes 0.0003 0 0.0001 0 0.0001 0 0.0008 0 0.0001 0 0.0006 0 0.0001 0 0.0004 0 0.0002 0
Endoplasmic Reticulum 0.0086 0.0014 0.0012 0.001 0.0026 0.0016 0.0065 0.0007 0.0007 0.0006 0.007 0.0014 0.004 0.0014 0.0016 0.0005 0.0008 0.0006
Endosome 0.0692 0.0031 0.0151 0.0462 0.0607 0.0139 0.0299 0.0018 0.001 0.002 0.0166 0.0015 0.0196 0.0012 0.01 0.0066 0.0202 0.0013
Golgi 0.0252 0.0017 0.0116 0.0057 0.0366 0.0151 0.0085 0.0001 0.0003 0.0024 0.0071 0.0001 0.0042 0.0002 0.0134 0.003 0.0153 0.0001
Lipid Particles 0.0069 0.0001 0.0027 0.002 0.004 0.0018 0.009 0 0.0001 0.0069 0.0071 0 0.0032 0 0.0096 0.0047 0.033 0
Mitochondria 0.0117 0.0198 0.0123 0.0009 0.0253 0.009 0.0133 0 0.0004 0.0006 0.0092 0.0002 0.0027 0 0.0184 0.0004 0.0521 0.0001
None 0.0046 0.0017 0.0013 0.0101 0.018 0.0009 0.0074 0.0014 0.0009 0.001 0.0028 0.0009 0.0045 0.0006 0.001 0.0027 0.0007 0.0011
Nuclear Periphery 0.0098 0.0003 0.0009 0.0005 0.0093 0.0004 0.0048 0.0004 0.0004 0.0005 0.0132 0.0004 0.0062 0.0003 0.0013 0.0004 0.0005 0.0002
Nucleolus 0.0003 0 0.0001 0.0001 0.0006 0 0.0003 0 0 0 0.0005 0 0.0001 0 0.0003 0 0.0003 0
Nucleus 0.0067 0.001 0.0015 0.0019 0.0074 0.0023 0.0029 0.0013 0.0014 0.0021 0.0054 0.0022 0.0026 0.0007 0.0011 0.001 0.001 0.0015
Peroxisomes 0.0108 0.001 0.0018 0.003 0.0222 0.0063 0.0058 0 0.0001 0.0135 0.0012 0 0.0126 0 0.0131 0.0193 0.0329 0
Punctate Nuclear 0.0035 0.0001 0.0005 0.0006 0.0075 0.0003 0.004 0.0002 0.0001 0.0006 0.0012 0.0002 0.0138 0.0001 0.0035 0.0023 0.0013 0.0001
Vacuole 0.0042 0.0003 0.0008 0.0061 0.0031 0.0006 0.0025 0.0003 0.0002 0.0003 0.0018 0.0003 0.0014 0.0002 0.0008 0.0006 0.0016 0.0002
Vacuole Periphery 0.0016 0.0001 0.0006 0.002 0.0011 0.0003 0.0011 0 0.0001 0.0001 0.0016 0.0001 0.0004 0 0.0005 0.0002 0.0009 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 78.5855 74.5517 70.9238 64.2623 60.302 70.5275 70.5471 75.0092 78.1382 81.8546
Translational Efficiency 1.2826 1.0931 1.0754 1.4724 1.6194 1.7262 1.3394 1.4413 1.4118 1.3509

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1680 440 2467 1543 1686 1006 122 991 3366 1446 2589 2534

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1057.68 1290.62 1557.36 1593.00 1078.75 1259.68 1862.30 1695.76 1068.23 1269.09 1571.73 1633.19
Standard Deviation 155.93 192.99 253.46 328.62 165.11 187.79 247.37 268.04 160.94 189.92 261.29 310.43
Intensity Change Log 2 — 0.287161 0.558199 0.590843 — 0.223697 0.787725 0.652571 — 0.255465 0.678651 0.622342

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000240 0.000835 0.000152 0.000447 0.000143 0.000289 0.000945 0.004183 0.000191 0.000455 0.000190 0.001908
Bud Neck 0.000885 0.000697 0.000734 0.001709 0.000676 0.000862 0.004839 0.001870 0.000780 0.000812 0.000928 0.001772
Bud Site 0.006254 0.009985 0.006739 0.010142 0.005053 0.011806 0.011693 0.016627 0.005652 0.011252 0.006973 0.012678
Cell Periphery 0.000067 0.000117 0.000104 0.000241 0.000107 0.000059 0.000447 0.000150 0.000087 0.000077 0.000121 0.000206
Cytoplasm 0.848698* 0.855118* 0.919485* 0.792423* 0.861884* 0.829397* 0.712360* 0.812076* 0.855302* 0.837223* 0.909724* 0.800109*
Cytoplasmic Foci 0.075624 0.077756 0.007487 0.013102 0.062257 0.059808 0.045947 0.021997 0.068929 0.065269 0.009299 0.016581
Eisosomes 0.000009 0.000008 0.000002 0.000006 0.000006 0.000008 0.000011 0.000029 0.000007 0.000008 0.000002 0.000015
Endoplasmic Reticulum 0.000897 0.000980 0.000085 0.000336 0.001561 0.001493 0.000848 0.000205 0.001230 0.001337 0.000121 0.000285
Endosome 0.002413 0.003449 0.000329 0.007893 0.001957 0.004440 0.004468 0.003889 0.002185 0.004139 0.000524 0.006327
Golgi 0.001392 0.003226 0.000566 0.008369 0.001468 0.003995 0.001569 0.009528 0.001430 0.003761 0.000613 0.008822
Lipid Particles 0.000681 0.000994 0.000545 0.001573 0.000587 0.000539 0.004597 0.001672 0.000634 0.000677 0.000736 0.001612
Mitochondria 0.000196 0.000067 0.000148 0.002484 0.000193 0.000913 0.000059 0.000104 0.000194 0.000656 0.000144 0.001553
Mitotic Spindle 0.000626 0.000493 0.002835 0.019103 0.001083 0.010365 0.007725 0.029787 0.000855 0.007361 0.003065 0.023282
None 0.002470 0.002272 0.002284 0.004220 0.005220 0.001598 0.002173 0.003105 0.003848 0.001803 0.002278 0.003784
Nuclear Periphery 0.000076 0.000037 0.000141 0.000136 0.000060 0.000225 0.000420 0.000158 0.000068 0.000167 0.000154 0.000145
Nuclear Periphery Foci 0.001966 0.004917 0.001352 0.002796 0.001913 0.002827 0.023150 0.003309 0.001940 0.003463 0.002379 0.002997
Nucleolus 0.000172 0.000072 0.000103 0.000235 0.000133 0.000103 0.000142 0.000123 0.000153 0.000094 0.000105 0.000191
Nucleus 0.021952 0.017208 0.039430 0.055255 0.030372 0.017762 0.063346 0.037459 0.026169 0.017593 0.040557 0.048295
Peroxisomes 0.000389 0.000628 0.000160 0.000391 0.000211 0.000601 0.000195 0.001865 0.000300 0.000609 0.000162 0.000967
Vacuole 0.034525 0.020446 0.017098 0.074991 0.024480 0.050543 0.114716 0.049823 0.029494 0.041385 0.021698 0.065148
Vacuole Periphery 0.000467 0.000697 0.000220 0.004147 0.000634 0.002368 0.000348 0.002042 0.000551 0.001859 0.000226 0.003324

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.38 0.42 -1.72 0.87 -2.95 -2.44 -6.68 -1.88 -1.66 -0.47 -1.79 -0.80 -2.37 -1.23 -2.23
Bud Neck 1.52 0.07 -7.63 -8.95 -8.04 -2.64 -7.77 -9.55 -7.81 5.57 -1.19 -4.23 -12.37 -11.02 -7.68
Bud Site -3.68 -0.03 -2.68 1.70 -2.34 -3.45 -6.03 -4.81 -0.13 2.00 -4.15 -1.55 -5.57 0.97 -3.21
Cell Periphery -5.57 -4.12 -7.62 -4.13 -3.89 1.61 -7.11 -1.52 -7.55 7.40 0.78 -2.38 -4.80 -8.54 -2.96
Cytoplasm -0.07 -10.58 14.90 10.55 24.07 6.82 11.15 15.33 7.05 -5.04 5.75 -7.38 21.55 11.02 26.80
Cytoplasmic Foci -0.79 25.43 22.79 13.33 -6.18 0.44 2.99 13.40 11.98 5.07 0.80 30.29 25.96 18.85 -7.87
Eisosomes 0.53 9.36 3.34 1.01 -10.06 -2.35 -6.18 -1.05 -0.68 0.19 -0.78 11.13 -0.53 -0.24 -2.32
Endoplasmic Reticulum -0.08 4.45 3.84 1.46 -3.46 0.51 1.99 3.97 1.56 5.45 0.24 5.45 5.23 1.89 -1.74
Endosome -1.07 12.66 -0.34 0.61 -3.16 -3.21 -5.00 1.90 3.94 5.69 -3.11 13.06 0.02 2.32 -3.57
Golgi -1.31 5.28 -1.24 0.32 -2.23 -1.50 0.35 -0.66 0.78 -0.76 -1.96 5.61 -1.38 0.69 -2.77
Lipid Particles -1.42 -1.94 -7.21 -4.30 -6.45 0.35 -7.46 -10.70 -10.46 4.24 -0.70 -5.09 -11.91 -10.74 -7.80
Mitochondria 2.26 1.40 -1.69 -2.10 -1.94 -1.11 2.86 3.13 1.42 0.72 -0.98 1.86 -1.42 0.09 -1.82
Mitotic Spindle 0.52 -2.92 -4.67 -4.79 -3.34 -3.79 -1.94 -4.44 -0.44 -0.64 -3.84 -2.91 -6.37 -1.29 -4.56
None 0.69 1.06 -0.14 -0.64 -0.81 6.79 4.61 5.63 -2.93 -0.81 6.62 5.27 3.66 -2.22 -0.72
Nuclear Periphery 1.07 -0.81 -3.67 -16.45 -0.66 -2.17 -9.08 -10.95 -0.65 5.50 -1.81 -1.23 -8.23 -1.27 -0.73
Nuclear Periphery Foci -6.09 0.81 -4.05 3.91 -4.38 -1.29 -8.64 -5.51 -1.49 7.94 -3.47 -2.70 -6.61 0.36 -1.79
Nucleolus 3.97 2.44 0.09 -5.78 -3.20 1.47 -2.21 -0.40 -1.61 1.78 3.46 2.59 -0.26 -4.42 -3.13
Nucleus 3.22 -18.04 -19.08 -19.68 -4.11 8.52 -5.33 -9.28 -15.28 2.48 7.94 -16.84 -20.14 -25.24 -2.59
Peroxisomes -1.13 3.99 2.53 2.00 -1.88 -4.22 -0.95 -1.67 0.08 -1.50 -3.39 4.01 -0.69 1.96 -2.33
Vacuole 3.63 3.10 -21.63 -22.01 -23.18 -11.33 -8.75 -20.51 -10.42 1.97 -9.09 -3.68 -30.05 -18.05 -27.05
Vacuole Periphery -0.52 4.61 -1.76 -1.03 -2.44 -1.83 2.95 0.82 2.03 -0.71 -1.97 5.67 -1.38 0.99 -2.73
Standard name
Human Ortholog
Description Peroxisomal malate dehydrogenase; catalyzes interconversion of malate and oxaloacetate; involved in the glyoxylate cycle
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Mdh3

Mdh3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mdh3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available