Standard name
Human Ortholog
Description Mitochondrial inner membrane half-type ABC transporter; required for respiratory growth at high temperature; localizes to vacuole membrane in response to H2O2; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0.05
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 – 0 0 0 0.05 0.05 0 0.31 – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0.05 0.07 0 0.12 – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0.07 0.08 0 0 – – 0 0.06 0 0.06 0 0
Golgi 0.06 0 0 0 0 0 0 0 – 0 0 0 0.35 0.36 0.56 0.15 – – 0.06 0.1 0 0 0.09 0.08
Mitochondria 0.85 0.94 0.93 0.94 0.86 0.94 0.91 0.92 – 0.95 0.95 0.93 0.57 0.55 0.35 0 – – 0.79 0.71 0.75 0.65 0.63 0.61
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nuclear Periphery 0.06 0 0.05 0 0.08 0.1 0.07 0.05 – 0.08 0.09 0.12 0 0 0 0.08 – – 0 0 0 0 0 0
Nucleolus 0.05 0 0.05 0 0.08 0.12 0.12 0.1 – 0.12 0.07 0.13 0 0 0 0 – – 0 0 0 0 0 0
Peroxisomes 0 0 0.06 0.06 0 0 0 0 – 0 0 0 0 0 0 0 – – 0.07 0 0.12 0.12 0.13 0.1
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.08 – – 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0.08 0 0 0.1 0 0 0 – 0 0 0 0.23 0.23 0.25 0.27 – – 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 2 1 2 0 0 0 0 – 0 0 1 2 2 1 0 – – 5 10 7 4 11 11
Bud 3 4 7 5 4 9 11 16 – 15 11 13 0 0 0 0 – – 0 2 0 2 1 3
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 1 0 1 1 3
Bud Site 0 2 0 0 1 5 5 5 – 12 9 10 0 0 0 0 – – – – – – – –
Cell Periphery 12 13 7 4 2 1 4 1 – 2 1 2 1 1 1 1 – – 0 0 0 0 1 0
Cytoplasm 2 3 9 4 6 5 5 10 – 2 3 4 7 7 4 8 – – 0 0 0 1 0 0
Endoplasmic Reticulum 0 4 0 1 0 2 0 3 – 4 6 4 6 9 1 3 – – 0 0 1 1 1 1
Endosome 13 3 7 1 10 8 6 6 – 0 1 0 9 11 3 0 – – 10 22 15 12 12 10
Golgi 19 9 4 3 5 0 0 2 – 0 2 0 45 47 53 4 – – 21 41 17 7 24 20
Mitochondria 272 364 383 212 272 403 508 509 – 520 440 505 73 73 33 0 – – 272 283 311 123 176 147
Nucleus 0 1 0 0 0 4 4 3 – 2 1 1 0 0 0 1 – – 0 0 0 0 0 0
Nuclear Periphery 19 12 22 10 24 42 41 26 – 45 44 67 4 3 1 2 – – 3 2 4 4 4 3
Nucleolus 17 12 19 3 25 50 69 54 – 65 34 68 3 3 2 0 – – 1 1 0 1 1 1
Peroxisomes 11 2 25 14 4 2 9 10 – 8 8 11 4 4 0 0 – – 22 12 50 22 36 23
SpindlePole 2 2 8 1 1 5 10 12 – 10 7 7 2 2 1 2 – – 1 0 1 2 0 1
Vac/Vac Membrane 52 31 14 9 33 14 23 13 – 11 19 9 30 30 24 7 – – 2 15 1 2 6 11
Unique Cell Count 321 389 414 225 318 429 559 554 550 464 541 128 132 95 26 343 398 417 190 282 241
Labelled Cell Count 423 464 506 269 387 550 695 670 696 586 702 186 192 124 28 343 398 417 190 282 241


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.9 8.0 7.2 7.1 7.3 6.1 6.1 6.5 – 5.8 6.0 6.2 8.7 8.7 8.9 7.1 – – 9.1 10.1 10.6
Std Deviation (1e-4) 1.5 1.5 1.6 1.6 1.4 1.6 1.8 1.8 – 1.6 1.8 1.8 1.4 1.4 1.5 1.9 – – 2.8 2.7 2.6
Intensity Change (Log2) – – – -0.04 0.01 -0.24 -0.26 -0.16 – -0.31 -0.28 -0.22 0.27 0.27 0.31 -0.03 – – 0.33 0.48 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 – –
Bud 0 0 0.4 0.3 1.2 – 1.1 0.7 0.8 0 0 0 0 – –
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 – –
Bud Site 0 0 0 0 0 – 3.0 0 0 0 0 0 0 – –
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 – –
Cytoplasm 0 -0.3 -1.1 -1.7 -0.4 – 0 -1.9 -1.9 0 0 0 0 – –
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 – –
Endosome 0 1.3 0.2 -0.8 -0.8 – 0 0 0 0 0 0 0 – –
Golgi 0 0 0 0 0 – 0 0 0 11.8 11.9 15.3 0 – –
Mitochondria 0.8 -3.1 0.8 -0.9 -0.4 – 1.3 1.4 0.5 -9.6 -10.0 -13.1 -13.6 – –
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 – –
Nuclear Periphery -0.5 1.2 2.5 1.3 -0.4 – 1.7 2.3 3.7 -1.0 -1.5 0 0 – –
Nucleolus -2.2 1.8 3.7 4.2 3.0 – 3.9 1.7 4.2 -1.1 -1.2 0 0 – –
Peroxisomes 0.1 -3.3 -4.6 -3.7 -3.5 – -3.9 -3.4 -3.2 -1.3 -1.3 0 0 – –
SpindlePole 0 0 -0.9 -0.2 0.3 – -0.1 -0.5 -0.8 0 0 0 0 – –
Vacuole 0.4 3.8 -0.1 0.6 -1.0 – -1.3 0.6 -1.7 7.3 7.1 7.3 0 – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0378 0.019 0.0303 0.0158 0.0027 0.007 0.0684 0.0328 0.0405 0.0006 0.0015 0.0055 – – – – – –
Bud 0.0006 0.0025 0.0015 0.001 0.0006 0.0002 0.0008 0.0008 0.0004 0.0002 0.0012 0.0001 – – – – – –
Bud Neck 0.0009 0.0016 0.004 0.0026 0.0006 0.0003 0.0006 0.0004 0.0002 0.0001 0.0002 0.0001 – – – – – –
Bud Periphery 0.001 0.0043 0.0019 0.0023 0.0004 0.0004 0.0012 0.0011 0.0006 0.0008 0.0017 0.0001 – – – – – –
Bud Site 0.0053 0.0162 0.0046 0.0028 0.0107 0.0003 0.0021 0.0039 0.0007 0.0001 0.0043 0.0001 – – – – – –
Cell Periphery 0.0005 0.0013 0.0011 0.0017 0.0001 0.0001 0.0003 0.0008 0.0002 0.0001 0.0003 0.0001 – – – – – –
Cytoplasm 0.0101 0.0011 0.0012 0.0047 0.002 0.0002 0.006 0.002 0.0023 0.0001 0.0006 0 – – – – – –
Cytoplasmic Foci 0.0149 0.0131 0.0106 0.0073 0.0165 0.0034 0.0211 0.0122 0.0047 0.0047 0.0068 0.0038 – – – – – –
Eisosomes 0.0008 0.0007 0.0025 0.0003 0.0001 0.0002 0.0065 0.0005 0.0009 0.0001 0.0001 0.0002 – – – – – –
Endoplasmic Reticulum 0.005 0.0005 0.0012 0.0056 0.0003 0.0004 0.0047 0.003 0.0013 0.0001 0.0002 0 – – – – – –
Endosome 0.0236 0.0329 0.0273 0.0272 0.0701 0.0126 0.086 0.0385 0.0249 0.0279 0.0214 0.0038 – – – – – –
Golgi 0.0247 0.0455 0.023 0.0308 0.0201 0.0216 0.038 0.0664 0.0233 0.0759 0.0136 0.0198 – – – – – –
Lipid Particles 0.0338 0.0127 0.01 0.009 0.0048 0.0066 0.0258 0.0107 0.0093 0.0021 0.0059 0.0016 – – – – – –
Mitochondria 0.7795 0.7937 0.846 0.8279 0.8388 0.9292 0.6082 0.7755 0.8487 0.8786 0.921 0.9363 – – – – – –
None 0.0043 0.0002 0.0003 0.0022 0.0001 0.0001 0.0078 0.0002 0.0011 0.0001 0.0003 0 – – – – – –
Nuclear Periphery 0.0165 0.0004 0.0016 0.0118 0.0002 0.0008 0.0551 0.0019 0.0134 0.0001 0.0005 0 – – – – – –
Nucleolus 0.0013 0.0022 0.0003 0.0088 0.0002 0.0001 0.0015 0.0004 0.0002 0.0001 0.0014 0 – – – – – –
Nucleus 0.0056 0.0008 0.0005 0.0084 0.0002 0.0001 0.007 0.0003 0.0035 0.0001 0.0015 0 – – – – – –
Peroxisomes 0.0107 0.014 0.0154 0.0046 0.0166 0.0076 0.0138 0.0368 0.0062 0.0012 0.005 0.0273 – – – – – –
Punctate Nuclear 0.009 0.0004 0.0004 0.0026 0.0005 0.0002 0.0309 0.0001 0.0012 0 0.0005 0 – – – – – –
Vacuole 0.0047 0.0226 0.0084 0.0174 0.0075 0.0032 0.0059 0.0056 0.0035 0.0031 0.0093 0.0002 – – – – – –
Vacuole Periphery 0.0092 0.0141 0.0079 0.0051 0.0068 0.0053 0.0084 0.0064 0.0132 0.004 0.0029 0.0008 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 27.7989 16.9918 19.8587 29.6924 32.0378 34.6701 33.2871 37.106 36.7709 34.864
Translational Efficiency 0.4725 0.6016 0.6348 0.419 0.3477 0.4888 0.3939 0.3579 0.4154 0.3816

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
324 1353 159 1653 2021 2266 1419 1502 2345 3619 1578 3155

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1114.18 881.67 1073.90 1017.63 893.40 945.36 983.55 1005.75 923.90 921.55 992.65 1011.97
Standard Deviation 199.45 166.52 135.97 140.19 125.31 139.66 131.39 153.97 157.59 153.40 134.64 147.03
Intensity Change Log 2 — -0.337672 -0.053123 -0.130769 — 0.081558 0.138692 0.170894 — -0.135957 0.035400 0.011310

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.025903 0.004185 0.003107 0.004470 0.000518 0.001389 0.002681 0.026863 0.004026 0.002434 0.002724 0.015131
Bud Neck 0.003311 0.002205 0.012451 0.002009 0.000256 0.000838 0.002081 0.001816 0.000678 0.001349 0.003126 0.001917
Bud Site 0.022385 0.008506 0.022812 0.025770 0.000524 0.002050 0.015405 0.041858 0.003545 0.004464 0.016151 0.033429
Cell Periphery 0.005049 0.000634 0.000505 0.000515 0.000099 0.000137 0.000231 0.001687 0.000783 0.000323 0.000258 0.001073
Cytoplasm 0.003639 0.001370 0.003466 0.004206 0.000318 0.000359 0.002412 0.008784 0.000776 0.000737 0.002519 0.006385
Cytoplasmic Foci 0.040085 0.028510 0.095790 0.031564 0.007614 0.015380 0.029561 0.032344 0.012100 0.020288 0.036234 0.031935
Eisosomes 0.000374 0.000090 0.000085 0.000068 0.000028 0.000042 0.000056 0.000196 0.000076 0.000060 0.000059 0.000129
Endoplasmic Reticulum 0.007927 0.000338 0.001640 0.001237 0.000241 0.000135 0.000433 0.001051 0.001303 0.000211 0.000555 0.001148
Endosome 0.062436 0.042513 0.118579 0.064702 0.020106 0.029666 0.048319 0.045682 0.025955 0.034469 0.055398 0.055647
Golgi 0.193211* 0.045196 0.092146 0.133208* 0.015638 0.026613 0.051088 0.101691 0.040172 0.033561 0.055225 0.118204*
Lipid Particles 0.001508 0.008501 0.017135 0.000781 0.002739 0.002637 0.001449 0.000456 0.002569 0.004829 0.003029 0.000627
Mitochondria 0.539025* 0.800433* 0.392351* 0.591127* 0.935097* 0.896271* 0.760318* 0.648987* 0.880374* 0.860441* 0.723242* 0.618672*
Mitotic Spindle 0.010380 0.004104 0.074047 0.031473 0.001203 0.002155 0.029948 0.022522 0.002471 0.002884 0.034392 0.027212
None 0.000547 0.000358 0.000343 0.000346 0.000288 0.000150 0.000228 0.000233 0.000324 0.000228 0.000239 0.000292
Nuclear Periphery 0.000424 0.000194 0.002939 0.000758 0.000100 0.000074 0.000209 0.000104 0.000145 0.000119 0.000484 0.000447
Nuclear Periphery Foci 0.000972 0.000671 0.003857 0.001056 0.000099 0.000120 0.000323 0.000142 0.000219 0.000326 0.000679 0.000621
Nucleolus 0.000131 0.001074 0.000466 0.000252 0.000040 0.000117 0.000111 0.000076 0.000052 0.000475 0.000147 0.000168
Nucleus 0.000287 0.000409 0.002175 0.000304 0.000048 0.000123 0.000134 0.000170 0.000081 0.000230 0.000340 0.000240
Peroxisomes 0.018335 0.040722 0.051334 0.008083 0.007194 0.012596 0.028947 0.049146 0.008733 0.023111 0.031203 0.027632
Vacuole 0.024222 0.005309 0.054585 0.048852 0.002727 0.004373 0.013237 0.008638 0.005697 0.004723 0.017403 0.029707
Vacuole Periphery 0.039849 0.004676 0.050187 0.049220 0.005123 0.004775 0.012828 0.007554 0.009921 0.004738 0.016593 0.029384

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 4.61 4.80 4.53 -0.23 -0.96 -3.14 -6.87 -10.89 -10.47 -9.92 2.10 1.78 -7.72 -9.63 -9.53
Bud Neck 1.47 -1.58 1.79 0.37 1.82 -3.88 -7.39 -7.84 -4.12 0.88 -3.48 -3.91 -6.63 -2.62 1.87
Bud Site 3.01 -0.04 -0.68 -6.49 -0.36 -3.27 -8.22 -14.34 -13.69 -7.78 -0.96 -6.58 -15.81 -15.31 -6.83
Cell Periphery 2.95 3.10 3.10 0.38 -0.10 -2.60 -7.69 -6.02 -5.87 -5.51 1.95 2.55 -1.21 -4.36 -6.32
Cytoplasm 1.51 0.19 -0.19 -4.32 -0.47 -0.18 -7.57 -13.59 -14.21 -10.21 0.28 -4.59 -10.85 -12.86 -7.97
Cytoplasmic Foci 2.64 -4.87 2.01 -1.20 5.92 -6.27 -11.40 -14.82 -9.07 -1.16 -6.40 -11.09 -14.24 -7.75 1.88
Eisosomes 4.68 4.72 5.06 3.43 2.02 -5.07 -9.63 -14.91 -13.49 -12.21 1.72 1.83 -5.05 -10.94 -11.14
Endoplasmic Reticulum 4.33 3.52 3.81 -6.27 1.40 0.76 -1.27 -2.92 -3.81 -2.50 3.95 2.63 0.50 -6.89 -3.98
Endosome 3.10 -4.63 -0.36 -6.06 4.94 -4.22 -9.97 -9.92 -5.91 0.83 -4.00 -10.10 -13.08 -9.56 -0.09
Golgi 10.70 5.65 4.15 -15.21 -3.23 -5.56 -11.43 -19.63 -16.79 -9.97 2.28 -3.99 -18.79 -23.37 -14.43
Lipid Particles -5.80 -2.89 1.65 6.64 3.03 0.16 2.30 4.86 4.61 2.77 -3.50 -0.65 4.69 7.90 3.73
Mitochondria -11.43 4.26 -2.25 16.98 -6.96 6.75 19.18 29.45* 24.59 9.02 2.97 15.86 31.81 31.37 9.81
Mitotic Spindle 2.64 -4.71 -6.15 -10.71 3.13 -2.19 -12.10 -12.54 -11.96 2.59 -0.71 -12.46 -15.68 -15.66 2.39
None 2.73 2.95 3.29 0.31 -0.10 1.29 0.59 0.54 -8.63 -0.46 1.04 0.92 0.37 -3.85 -4.32
Nuclear Periphery 1.28 -2.71 -1.47 -3.55 2.36 1.25 -2.64 -0.12 -0.81 2.02 0.74 -3.28 -3.56 -3.94 0.28
Nuclear Periphery Foci 0.60 -2.31 -0.15 -1.11 2.35 -0.85 -2.70 -1.23 -0.55 2.08 -1.12 -2.99 -2.44 -1.75 0.31
Nucleolus -1.49 -2.67 -1.02 1.29 1.57 -1.05 -2.05 -0.61 0.40 0.53 -1.76 -2.71 -2.03 1.23 -0.31
Nucleus -0.45 -1.39 -0.04 0.47 1.39 -1.15 -3.04 -1.08 -0.40 -0.31 -1.66 -1.86 -2.19 -0.13 0.65
Peroxisomes -4.23 -3.19 2.50 9.37 4.49 -3.56 -7.80 -11.82 -10.15 -4.69 -8.08 -8.20 -9.30 -1.99 1.20
Vacuole 4.76 -3.41 -5.29 -16.67 0.69 -2.83 -9.96 -6.04 -4.18 3.40 1.32 -8.38 -15.25 -16.79 -6.45
Vacuole Periphery 5.85 -1.08 -1.47 -19.05 0.10 0.53 -6.68 -3.28 -4.08 4.50 5.11 -4.29 -12.11 -18.66 -7.23
Standard name
Human Ortholog
Description Mitochondrial inner membrane half-type ABC transporter; required for respiratory growth at high temperature; localizes to vacuole membrane in response to H2O2; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis
Localization
Cell Percentages mitochondrion (96%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Mdl2

Mdl2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mdl2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available