| Standard name | |
|---|---|
| Human Ortholog | |
| Description (SGD) | Moonlighting protein component of both the ERMES and SAM complex; subunit of the ERMES complex that acts as a molecular tether between the mitochondria and the ER, necessary for both efficient phospholipid exchange between organelles and for mitophagy; SAM/TOB complex subunit that functions in the assembly of outer membrane beta-barrel proteins; involved in mitochondrial inheritance and morphology; ERMES complex often co-localizes with peroxisomes adjacent to mitochondria-ER junctions |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
| Vac/Vac Membrane | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | 0 |
| Unique Cell Count | 1 | 1 | ||||||||||||||||||||||
| Labelled Cell Count | 1 | 1 | ||||||||||||||||||||||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Std Deviation (1e-4) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|
External localization resources
Images
Protein Concentration and Protein Localization Data
| R1 | R2 | R3 | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasmic Foci | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Eisosomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Lipid Particles | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| None | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Punctate Nuclear | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Sequencing Data
| R1 | R2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Gene Expression | 4.4672 | 5.9901 | 3.9511 | 7.6255 | 4.8266 | 5.953 | 8.412 | 8.6298 | 7.5869 | 9.3417 |
| Translational Efficiency | 0.6755 | 0.8616 | 1.3095 | 0.4567 | 0.7339 | 0.7726 | 0.7382 | 0.5304 | 0.7111 | 0.6362 |
Hit Data
| Dataset | Hit |
|---|---|
| Protein Concentration | – |
| Protein Localization | – |
| Gene Expression | ✘ |
| Translational Efficiency | ✘ |
Micrographs
Cell Count
Number of cells analysed for each strain. R1, R2: biological replicates; R1 & R2: both replicates combined.
| R1 | R2 | R1 & R2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
| 5 | 38 | N/A | N/A | 1 | N/A | N/A | N/A | 6 | 38 | 0 | 0 |
Protein Abundance
Mean and standard deviation of cell GFP intensity, and intensity change (log2) of each mutant relative to wild type.
| R1 | R2 | R1 & R2 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
| Mean | 360.63 | 312.65 | N/A | N/A | 283.75 | N/A | N/A | N/A | 347.82 | 312.65 | — | — |
| Standard Deviation | 2.26 | 6.66 | N/A | N/A | — | N/A | N/A | N/A | 28.73 | 6.66 | — | — |
| Intensity Change Log 2 | — | -0.205971 | N/A | N/A | — | N/A | N/A | N/A | — | — | — | — |
Localization Score (DeepLoc)
Localization score for each compartment. Values highlighted in green denote significant localization scores (marked * in the plot).
| R1 | R2 | R1 & R2 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
| Actin | 0.000068 | 0.000024 | N/A | N/A | 0.000003 | N/A | N/A | N/A | 0.000057 | 0.000024 | N/A | N/A |
| Bud Neck | 0.000006 | 0.000027 | N/A | N/A | 0.000000 | N/A | N/A | N/A | 0.000005 | 0.000027 | N/A | N/A |
| Bud Site | 0.000029 | 0.000028 | N/A | N/A | 0.000001 | N/A | N/A | N/A | 0.000024 | 0.000028 | N/A | N/A |
| Cell Periphery | 0.000215 | 0.000065 | N/A | N/A | 0.000012 | N/A | N/A | N/A | 0.000181 | 0.000065 | N/A | N/A |
| Cytoplasm | 0.000072 | 0.000050 | N/A | N/A | 0.000004 | N/A | N/A | N/A | 0.000060 | 0.000050 | N/A | N/A |
| Cytoplasmic Foci | 0.000011 | 0.000018 | N/A | N/A | 0.000001 | N/A | N/A | N/A | 0.000009 | 0.000018 | N/A | N/A |
| Eisosomes | 0.000402 | 0.000041 | N/A | N/A | 0.000006 | N/A | N/A | N/A | 0.000336 | 0.000041 | N/A | N/A |
| Endoplasmic Reticulum | 0.000001 | 0.000001 | N/A | N/A | 0.000000 | N/A | N/A | N/A | 0.000001 | 0.000001 | N/A | N/A |
| Endosome | 0.000000 | 0.000002 | N/A | N/A | 0.000000 | N/A | N/A | N/A | 0.000000 | 0.000002 | N/A | N/A |
| Golgi | 0.000005 | 0.000006 | N/A | N/A | 0.000001 | N/A | N/A | N/A | 0.000004 | 0.000006 | N/A | N/A |
| Lipid Particles | 0.000018 | 0.000039 | N/A | N/A | 0.000004 | N/A | N/A | N/A | 0.000016 | 0.000039 | N/A | N/A |
| Mitochondria | 0.000014 | 0.000012 | N/A | N/A | 0.000004 | N/A | N/A | N/A | 0.000012 | 0.000012 | N/A | N/A |
| Mitotic Spindle | 0.000131 | 0.000377 | N/A | N/A | 0.000087 | N/A | N/A | N/A | 0.000123 | 0.000377 | N/A | N/A |
| None | 0.998840* | 0.999114* | N/A | N/A | 0.999855* | N/A | N/A | N/A | 0.999010* | 0.999114* | N/A | N/A |
| Nuclear Periphery | 0.000000 | 0.000000 | N/A | N/A | 0.000000 | N/A | N/A | N/A | 0.000000 | 0.000000 | N/A | N/A |
| Nuclear Periphery Foci | 0.000002 | 0.000054 | N/A | N/A | 0.000004 | N/A | N/A | N/A | 0.000002 | 0.000054 | N/A | N/A |
| Nucleolus | 0.000002 | 0.000054 | N/A | N/A | 0.000005 | N/A | N/A | N/A | 0.000003 | 0.000054 | N/A | N/A |
| Nucleus | 0.000008 | 0.000016 | N/A | N/A | 0.000003 | N/A | N/A | N/A | 0.000007 | 0.000016 | N/A | N/A |
| Peroxisomes | 0.000176 | 0.000071 | N/A | N/A | 0.000009 | N/A | N/A | N/A | 0.000148 | 0.000071 | N/A | N/A |
| Vacuole | 0.000000 | 0.000002 | N/A | N/A | 0.000000 | N/A | N/A | N/A | 0.000000 | 0.000002 | N/A | N/A |
| Vacuole Periphery | 0.000000 | 0.000000 | N/A | N/A | 0.000000 | N/A | N/A | N/A | 0.000000 | 0.000000 | N/A | N/A |
Localization Changes (T-score)
Change in localization of each mutant relative to its control (e.g. UBP2_WT: ubp2Δ vs wild type). Values highlighted in yellow (increase) or blue (decrease) denote significant changes (marked * in the plot).
| R1 | R2 | R1 & R2 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| UBP2_ |
UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
UBP2_ |
UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
UBP2_ |
UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
|
| Actin | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Bud Neck | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Bud Site | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Cell Periphery | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Cytoplasm | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Cytoplasmic Foci | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Eisosomes | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Endoplasmic Reticulum | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Endosome | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Golgi | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Lipid Particles | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Mitochondria | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Mitotic Spindle | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| None | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Nuclear Periphery | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Nuclear Periphery Foci | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Nucleolus | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Nucleus | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Peroxisomes | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Vacuole | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Vacuole Periphery | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
Endocytosis
| Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
|---|---|---|---|---|
| 37℃ | ||||
| RT |
Cell Cycle Omics
CYCLoPs (Mdm10-GFP)
| Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
|---|
| Gene | Images |
|---|
| Gene | Images |
|---|
Images are not yet available
Images are not yet available
