Standard name
Human Ortholog
Description Mitochondrial protein; forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.08 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.05 0.1 0.41 0.29 0.3 0 0.05 0.06 0 0 0 0 0.28 0.43 0.52 0.56 0.53 0.38 0.06 0.08 0.06 0.12 0.16 0.17
Mitochondria 0.96 0.83 0.66 0.92 0.8 0.81 0.77 0.79 0.85 0.88 0.92 0.85 0.82 0.76 0.62 0.11 0.17 0.17 0.84 0.79 0.78 0.75 0.65 0.69
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0 0 0 0 0.07 0.09 0.07 0.1 0.06 0.05 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0 0 0 0.1 0.13 0.19 0.15 0.13 0.13 0.09 0.15 0 0 0 0.12 0.06 0.11 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.1 0.16 0 0.1 0.08 0.05 0.07 0 0 0 0.05 0.14 0.11 0.16 0.16 0.18 0.26 0 0 0 0.06 0.07 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 5 4 4 2 1 2 3 1 0 1 1 2 6 0 17 13 5 3 13 7 1 10 8
Bud 0 0 0 2 1 5 5 5 2 5 8 5 1 0 0 2 0 1 0 5 0 1 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 – – – – – –
Cell Periphery 0 13 5 1 2 0 1 1 2 1 1 0 4 6 5 13 10 4 0 0 1 0 0 0
Cytoplasm 0 10 0 2 3 4 6 5 1 1 4 6 5 8 13 4 7 2 0 0 0 0 1 0
Endoplasmic Reticulum 1 2 0 0 0 0 0 1 2 1 1 1 0 2 1 4 3 2 0 1 0 0 1 0
Endosome 0 1 1 0 1 3 4 2 3 4 0 1 2 2 2 1 1 3 2 6 1 0 4 1
Golgi 11 44 74 58 63 10 20 15 3 12 4 11 82 160 184 106 91 50 12 40 13 16 40 45
Mitochondria 206 353 118 184 165 246 295 206 210 292 302 307 243 283 220 21 30 22 184 382 170 104 170 177
Nucleus 0 2 0 0 2 0 3 3 0 0 3 4 1 0 1 3 1 3 0 0 0 0 1 1
Nuclear Periphery 10 12 4 3 3 20 35 17 25 19 16 32 3 9 5 3 4 4 1 5 2 0 1 0
Nucleolus 13 19 8 9 20 40 71 39 32 43 31 53 10 13 9 22 10 14 2 4 2 3 3 1
Peroxisomes 4 4 3 1 2 4 2 0 0 5 3 0 5 5 8 8 4 3 6 3 11 1 1 2
SpindlePole 0 1 0 0 0 2 4 1 1 3 1 1 0 0 1 4 1 2 0 4 0 0 1 1
Vac/Vac Membrane 10 42 29 8 21 25 19 17 10 13 14 17 40 40 58 31 31 34 3 14 4 8 18 15
Unique Cell Count 215 424 180 201 207 303 383 260 246 331 327 361 296 371 355 189 172 131 219 484 218 139 261 259
Labelled Cell Count 255 508 246 272 285 360 467 315 292 399 389 439 398 534 507 240 207 151 219 484 218 139 261 259


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.4 22.4 20.4 20.4 19.6 17.2 16.2 15.3 14.0 13.7 12.3 12.5 21.1 19.8 19.0 26.2 26.0 23.7 21.5 21.1 22.0
Std Deviation (1e-4) 4.7 5.7 4.0 3.9 3.9 4.1 4.0 3.6 3.2 3.5 3.4 3.2 4.4 4.4 4.0 8.9 8.7 9.0 6.7 5.8 5.1
Intensity Change (Log2) – – – 0.0 -0.06 -0.24 -0.33 -0.41 -0.54 -0.57 -0.73 -0.7 0.05 -0.04 -0.1 0.36 0.35 0.22 0.08 0.05 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 2.8 2.3 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1.8 1.4 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -2.5 -2.2 -10.6 -10.6 -9.1 -10.6 -10.8 -11.9 -11.5 -3.0 0.4 2.3 2.9 2.2 -0.5
Mitochondria 6.2 3.1 3.9 2.9 3.2 4.8 6.1 7.7 5.2 4.1 2.7 -0.8 -10.8 -9.1 -8.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 2.1 3.0 2.1 3.2 1.8 1.5 2.9 0 0 0 0 0 0
Nucleolus 0 2.0 3.1 4.5 3.5 3.0 3.1 2.0 3.5 -0.6 -0.5 -1.2 2.5 0.6 2.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1.4 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -4.0 -1.7 -2.7 -4.4 -3.2 -4.3 -4.8 -4.6 -4.5 -0.8 -1.8 0.1 0.1 0.5 2.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 28.3352 27.6837 29.5993 25.5176 24.5475 25.7985 28.3562 27.1431 30.6904 27.4622 26.3282 25.8371 30.8873 30.2255 30.1725 28.3937 28.946 29.2582
Actin 0.0028 0.0027 0.0003 0.0002 0.0026 0.0001 0.0004 0.0005 0.0003 0.0002 0.0003 0.0002 0.0003 0.0002 0.0004 0.0001 0.0001 0.0001
Bud 0 0 0 0 0 0 0 0 0 0 0.0001 0 0 0 0 0.0015 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Periphery 0 0.0001 0.0001 0 0.0001 0 0.0001 0.0001 0.0002 0 0.0003 0.0001 0 0.0001 0.0001 0.0035 0 0
Bud Site 0 0.0001 0 0 0 0 0 0 0 0 0.0001 0 0 0 0.0001 0.0001 0 0
Cell Periphery 0.0002 0.0002 0.0004 0 0.0001 0.0001 0.0001 0.0004 0.0001 0 0.0002 0.0001 0.0001 0.0003 0.0002 0.0004 0.0001 0.0001
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasmic Foci 0.0003 0.0003 0.0001 0.0008 0 0.0001 0.0001 0.0001 0.0001 0 0.0002 0 0.0001 0.0001 0.0002 0 0 0
Eisosomes 0.0004 0.0007 0.0004 0.0002 0.0003 0.0002 0.0002 0.0003 0.001 0.0003 0.0001 0.0002 0.0004 0.0003 0.0002 0.0001 0.0004 0.0002
Endoplasmic Reticulum 0 0 0.0002 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.0004 0.0022 0.0009 0.0053 0.0002 0.0007 0.0009 0.001 0.0006 0.0001 0.0018 0.0008 0.0004 0.0006 0.0009 0.0003 0.0002 0.0005
Golgi 0.002 0.0068 0.004 0.0451 0.0005 0.0104 0.0042 0.0036 0.0076 0.0003 0.0247 0.0076 0.0021 0.0027 0.0041 0.0001 0.0013 0.0042
Lipid Particles 0.0035 0.0016 0.0016 0.0004 0.0001 0.0049 0.0028 0.0051 0.0014 0.0001 0.0016 0.0006 0.0015 0.004 0.0016 0.0001 0.0003 0.001
Mitochondria 0.9749 0.9761 0.9808 0.9472 0.9897 0.9747 0.9846 0.9722 0.985 0.997 0.9661 0.9754 0.9886 0.9652 0.986 0.9784 0.9963 0.985
None 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0.0001 0 0 0 0.0004 0 0 0 0 0.0001 0 0 0 0 0 0.0001
Nucleolus 0.0002 0.0003 0.0001 0 0 0 0.0001 0.0002 0.0001 0 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0001 0
Nucleus 0 0 0 0 0 0 0 0.0001 0 0 0 0 0 0 0 0.0002 0 0
Peroxisomes 0.0043 0.002 0.0002 0 0.0003 0.0001 0.0004 0.0007 0.0003 0.0001 0.0004 0.0001 0.0003 0.0005 0.0008 0.0002 0.0002 0.0001
Punctate Nuclear 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.0021 0.0011 0.0023 0.0001 0.0003 0.0005 0.0006 0.002 0.0005 0.0001 0.0009 0.0016 0.0005 0.0042 0.0007 0.0077 0.0002 0.0006
Vacuole Periphery 0.0085 0.0058 0.0083 0.0007 0.0057 0.0081 0.005 0.0137 0.0026 0.0018 0.0031 0.013 0.0057 0.0214 0.0045 0.0072 0.0009 0.0083

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 62.1546 60.1449 53.5561 51.8395 51.9241 73.0072 60.0885 58.0484 62.54 56.0734
Translational Efficiency 1.2221 1.2696 1.2116 1.266 1.189 1.0705 1.0569 1.2125 1.1284 1.1921

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
402 309 1218 782 2118 1424 317 159 2520 1733 1535 941

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1118.52 1484.66 1738.51 2085.50 1518.00 1670.27 1769.84 1576.49 1454.27 1637.18 1744.98 1999.49
Standard Deviation 231.52 364.19 330.80 522.66 318.92 385.73 286.78 339.45 339.75 388.52 322.45 531.85
Intensity Change Log 2 — 0.408542 0.636260 0.898802 — 0.137910 0.221447 0.054544 — 0.258973 0.412158 0.473993

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003594 0.012272 0.001557 0.019895 0.000724 0.002443 0.010946 0.006647 0.001182 0.004196 0.003496 0.017656
Bud Neck 0.003553 0.005030 0.001171 0.002067 0.000432 0.001065 0.005380 0.012747 0.000929 0.001772 0.002041 0.003872
Bud Site 0.005461 0.006715 0.000559 0.002409 0.000499 0.001113 0.003530 0.026474 0.001290 0.002112 0.001173 0.006475
Cell Periphery 0.000537 0.000649 0.000056 0.000438 0.000053 0.000094 0.000110 0.002197 0.000130 0.000193 0.000067 0.000735
Cytoplasm 0.001142 0.000868 0.000149 0.000312 0.000067 0.000237 0.000484 0.004746 0.000239 0.000349 0.000218 0.001061
Cytoplasmic Foci 0.014849 0.028667 0.015421 0.008972 0.006203 0.012981 0.043467 0.017551 0.007582 0.015778 0.021213 0.010421
Eisosomes 0.000575 0.003330 0.000331 0.001792 0.000774 0.000958 0.000747 0.000383 0.000742 0.001381 0.000417 0.001554
Endoplasmic Reticulum 0.000319 0.000331 0.000087 0.000303 0.000055 0.000146 0.000263 0.002059 0.000097 0.000179 0.000123 0.000600
Endosome 0.005561 0.009398 0.001150 0.001543 0.000446 0.001938 0.006449 0.028093 0.001262 0.003268 0.002245 0.006029
Golgi 0.007270 0.039064 0.020371 0.022716 0.004998 0.021331 0.048356 0.058396 0.005360 0.024493 0.026151 0.028745
Lipid Particles 0.005947 0.009842 0.004939 0.002734 0.001993 0.005770 0.006609 0.020704 0.002624 0.006496 0.005284 0.005771
Mitochondria 0.898881* 0.825196* 0.910922* 0.901859* 0.970346* 0.930895* 0.818228* 0.682425* 0.958945* 0.912048* 0.891779* 0.864782*
Mitotic Spindle 0.001694 0.001332 0.001515 0.002107 0.000127 0.000236 0.007156 0.009623 0.000377 0.000431 0.002680 0.003377
None 0.001752 0.003689 0.003595 0.002789 0.002868 0.002902 0.001868 0.002356 0.002690 0.003042 0.003238 0.002716
Nuclear Periphery 0.000182 0.000215 0.001325 0.000340 0.000258 0.000237 0.000742 0.010501 0.000246 0.000233 0.001205 0.002057
Nuclear Periphery Foci 0.000573 0.000547 0.000506 0.000282 0.000171 0.000246 0.000512 0.003186 0.000235 0.000300 0.000507 0.000773
Nucleolus 0.002301 0.001840 0.000992 0.000660 0.000299 0.002127 0.000655 0.003682 0.000619 0.002076 0.000922 0.001171
Nucleus 0.000798 0.000343 0.000057 0.000306 0.000052 0.000339 0.000112 0.003446 0.000171 0.000340 0.000069 0.000836
Peroxisomes 0.020823 0.032261 0.006815 0.009383 0.004590 0.004134 0.029269 0.005492 0.007179 0.009149 0.011452 0.008725
Vacuole 0.014356 0.013017 0.008112 0.004942 0.002016 0.004859 0.006995 0.045852 0.003984 0.006314 0.007881 0.011855
Vacuole Periphery 0.009833 0.005393 0.020370 0.014152 0.003032 0.005951 0.008122 0.053437 0.004116 0.005852 0.017841 0.020790

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.37 1.35 -4.99 -2.13 -6.30 -4.54 -4.22 -3.07 -2.17 1.32 -5.48 -3.92 -6.72 -5.42 -5.68
Bud Neck -1.12 2.95 1.58 2.59 -2.38 -2.74 -3.79 -4.26 -4.03 -2.32 -2.84 -3.27 -4.93 -3.38 -2.86
Bud Site -0.44 3.28 1.92 1.75 -3.85 -1.50 -2.50 -3.46 -3.38 -3.04 -1.39 0.29 -3.75 -3.04 -3.85
Cell Periphery -0.36 2.62 0.46 0.80 -3.99 -2.50 -4.20 -2.26 -2.22 -2.21 -1.18 2.06 -3.28 -2.92 -3.66
Cytoplasm 0.63 2.59 2.11 2.47 -2.80 -2.82 -6.92 -1.59 -1.55 -1.49 -1.34 0.33 -1.63 -1.47 -1.67
Cytoplasmic Foci -2.73 -0.19 1.97 4.32 3.12 -4.95 -7.65 -3.47 -1.34 4.49 -5.69 -7.81 -2.02 2.98 5.29
Eisosomes -3.45 2.19 -4.99 1.87 -6.65 -1.88 0.28 4.52 5.17 3.37 -3.87 6.22 -4.32 -0.71 -6.16
Endoplasmic Reticulum -0.12 3.02 0.14 0.33 -4.23 -3.46 -4.16 -2.17 -2.07 -1.94 -3.10 -1.35 -3.10 -2.58 -2.94
Endosome -1.54 4.16 3.69 3.44 -1.34 -4.44 -4.08 -4.87 -4.60 -3.69 -3.84 -2.59 -4.56 -2.42 -3.50
Golgi -4.94 -5.14 -5.35 2.43 -0.73 -7.41 -7.32 -4.83 -3.28 -0.78 -8.91 -9.73 -8.17 -1.20 -0.73
Lipid Particles -1.29 0.44 1.37 3.30 2.48 -3.91 -3.69 -4.06 -3.19 -2.96 -4.10 -3.76 -2.93 0.55 -0.45
Mitochondria 3.77 -1.01 -0.19 -4.30 1.10 8.06 9.70 10.01 8.58 4.24 8.74 11.54 11.13 5.12 2.90
Mitotic Spindle 0.36 0.26 -0.54 -0.87 -1.05 -2.31 -3.46 -3.32 -3.28 -0.74 -0.30 -4.61 -4.43 -4.31 -0.94
None -3.47 -8.14 -4.34 1.57 3.06 -0.17 4.60 1.71 1.87 -1.65 -1.73 -2.76 -0.12 1.44 2.36
Nuclear Periphery -0.61 -6.95 -2.95 -2.17 5.86 0.23 -3.02 -2.02 -2.02 -1.92 0.16 -6.44 -2.10 -2.12 -0.98
Nuclear Periphery Foci 0.11 0.37 1.08 1.01 1.14 -0.99 -3.02 -3.61 -3.52 -3.18 -0.85 -3.74 -2.76 -2.44 -1.43
Nucleolus 0.50 1.72 2.07 1.56 0.65 -2.50 -1.84 -2.12 -0.88 -1.89 -2.33 -1.08 -1.55 1.22 -0.69
Nucleus 1.41 2.42 1.14 -0.10 -1.38 -1.66 -1.69 -1.22 -1.11 -1.19 -1.14 1.96 -1.40 -1.01 -1.61
Peroxisomes -1.55 3.35 2.62 3.47 -1.02 0.47 -4.30 -0.31 -0.48 3.79 -1.27 -2.24 -0.79 0.20 1.14
Vacuole 0.33 2.46 3.66 2.35 2.45 -3.77 -3.68 -5.53 -5.16 -4.85 -2.50 -4.35 -4.71 -3.13 -2.32
Vacuole Periphery 2.20 -4.23 -1.60 -4.09 2.39 -4.72 -3.54 -5.95 -5.60 -5.28 -2.85 -9.61 -7.37 -6.57 -1.13
Standard name
Human Ortholog
Description Mitochondrial protein; forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome
Localization
Cell Percentages mitochondrion (99%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Mdm38

Mdm38


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mdm38-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available