Standard name | |
---|---|
Human Ortholog | |
Description | Phosphorylated protein of the mitochondrial outer membrane; localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 5 | 11 | 5 | 5 | 1 | 3 | 0 | 1 | 2 | 1 | 1 | 2 | 4 | 4 | 1 | 11 | 20 | 12 | 24 | 16 | 13 | 21 | 18 | 24 |
Bud | 12 | 6 | 7 | 8 | 5 | 12 | 10 | 12 | 17 | 9 | 9 | 16 | 7 | 3 | 2 | 1 | 2 | 1 | 28 | 9 | 6 | 8 | 12 | 18 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 12 | 9 | 5 | 5 | 10 | 19 |
Bud Site | 1 | 1 | 1 | 1 | 0 | 2 | 2 | 4 | 3 | 3 | 1 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 2 | 3 | 3 | 1 | 1 | 1 | 3 | 5 | 4 | 1 | 2 | 1 | 6 | 2 | 9 | 8 | 12 | 0 | 0 | 0 | 0 | 2 | 4 |
Cytoplasm | 137 | 96 | 116 | 63 | 102 | 65 | 62 | 79 | 42 | 29 | 26 | 41 | 229 | 286 | 280 | 69 | 92 | 65 | 82 | 65 | 52 | 98 | 191 | 134 |
Endoplasmic Reticulum | 5 | 15 | 13 | 2 | 5 | 5 | 2 | 2 | 5 | 4 | 7 | 3 | 6 | 16 | 12 | 6 | 2 | 2 | 4 | 2 | 7 | 2 | 4 | 6 |
Endosome | 13 | 3 | 5 | 5 | 17 | 15 | 21 | 14 | 2 | 3 | 0 | 2 | 24 | 21 | 31 | 7 | 12 | 5 | 60 | 17 | 28 | 25 | 24 | 29 |
Golgi | 0 | 1 | 1 | 2 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 3 | 10 | 6 | 1 | 3 | 1 | 1 | 3 | 4 |
Mitochondria | 130 | 18 | 37 | 55 | 52 | 145 | 212 | 220 | 191 | 190 | 176 | 246 | 7 | 24 | 28 | 7 | 6 | 7 | 60 | 33 | 67 | 35 | 67 | 56 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 9 | 3 | 1 | 1 | 3 | 0 | 1 | 0 | 3 | 4 | 1 | 0 | 0 | 0 | 2 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 2 | 0 | 3 | 3 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 4 | 0 | 0 | 0 | 1 | 1 | 10 | 5 | 2 | 3 | 1 | 3 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 |
Peroxisomes | 3 | 1 | 0 | 3 | 2 | 2 | 4 | 2 | 1 | 2 | 0 | 3 | 1 | 2 | 4 | 1 | 2 | 2 | 2 | 0 | 1 | 1 | 1 | 1 |
SpindlePole | 0 | 3 | 2 | 3 | 4 | 7 | 8 | 18 | 6 | 4 | 4 | 6 | 0 | 2 | 3 | 5 | 12 | 5 | 28 | 7 | 7 | 12 | 14 | 14 |
Vac/Vac Membrane | 30 | 4 | 11 | 7 | 13 | 17 | 23 | 26 | 17 | 8 | 8 | 10 | 17 | 19 | 20 | 3 | 13 | 12 | 17 | 9 | 8 | 6 | 11 | 16 |
Unique Cell Count | 287 | 141 | 178 | 116 | 173 | 217 | 289 | 317 | 229 | 218 | 205 | 271 | 276 | 361 | 364 | 105 | 161 | 105 | 340 | 185 | 211 | 230 | 373 | 347 |
Labelled Cell Count | 341 | 162 | 201 | 157 | 205 | 277 | 365 | 399 | 298 | 262 | 236 | 342 | 297 | 387 | 384 | 127 | 187 | 131 | 340 | 185 | 211 | 230 | 373 | 347 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.9 | 5.7 | 5.0 | 4.7 | 5.0 | 4.4 | 4.0 | 4.4 | 4.1 | 3.8 | 3.9 | 3.7 | 6.1 | 6.1 | 5.9 | 8.5 | 9.9 | 10.2 | 5.4 | 5.2 | 6.0 |
Std Deviation (1e-4) | 0.7 | 1.1 | 0.9 | 1.0 | 1.2 | 1.4 | 1.2 | 1.6 | 1.3 | 0.9 | 1.3 | 1.1 | 1.0 | 1.3 | 1.3 | 1.7 | 1.9 | 2.2 | 0.9 | 1.1 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.11 | 0.0 | -0.18 | -0.33 | -0.2 | -0.31 | -0.43 | -0.37 | -0.43 | 0.27 | 0.26 | 0.23 | 0.75 | 0.97 | 1.01 | 0.1 | 0.04 | 0.25 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.7 | 3.4 | 2.9 |
Bud | 0 | -0.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.8 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 3.5 |
Cytoplasm | -1.9 | -1.2 | -7.0 | -9.4 | -8.8 | -9.6 | -10.7 | -10.6 | -10.9 | 4.3 | 3.5 | 2.9 | 0.1 | -1.5 | -0.6 |
Endoplasmic Reticulum | 0 | -1.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.7 | -1.4 | -2.1 | -0.5 | -2.7 | -2.0 |
Endosome | 0 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 | 1.5 | 2.5 | 0 | 2.0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 0 |
Mitochondria | 0 | 2.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -6.4 | -4.9 | -4.4 | -3.2 | -4.7 | -3.2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 0 |
Vacuole | -0.1 | 0.5 | 0.6 | 0.7 | 0.8 | 0.5 | -1.2 | -1.0 | -1.2 | 0 | -0.4 | -0.3 | -1.2 | 0.7 | 1.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.8652 | 3.6884 | 2.8089 | 3.1091 | 3.0597 | 3.6187 | 3.5754 | 3.8367 | 3.3757 | 3.074 | 3.5794 | 3.7 | 4.807 | 4.4434 | 4.6422 | 4.8387 | 4.1249 | 5.1188 |
Actin | 0.0397 | 0.0124 | 0.0362 | 0.0342 | 0.0166 | 0.0331 | 0.0312 | 0.0326 | 0.0359 | 0.1693 | 0.0662 | 0.0286 | 0.0604 | 0.0018 | 0.042 | 0.0027 | 0.0117 | 0.0256 |
Bud | 0.003 | 0.0063 | 0.0097 | 0.0335 | 0.0538 | 0.0057 | 0.005 | 0.0054 | 0.0252 | 0.0237 | 0.0932 | 0.0048 | 0.0011 | 0.001 | 0.0063 | 0.0006 | 0.0197 | 0.0014 |
Bud Neck | 0.0233 | 0.0038 | 0.011 | 0.0083 | 0.0083 | 0.244 | 0.007 | 0.0008 | 0.0034 | 0.0021 | 0.0635 | 0.1413 | 0.0295 | 0.0014 | 0.0021 | 0.001 | 0.0014 | 0.174 |
Bud Periphery | 0.0031 | 0.0062 | 0.0114 | 0.0341 | 0.0836 | 0.0124 | 0.0059 | 0.0018 | 0.0194 | 0.0293 | 0.0757 | 0.0054 | 0.001 | 0.0003 | 0.0059 | 0.0005 | 0.013 | 0.0013 |
Bud Site | 0.0387 | 0.0476 | 0.0229 | 0.0043 | 0.0072 | 0.0029 | 0.0302 | 0.0455 | 0.0205 | 0.0094 | 0.0127 | 0.0055 | 0.0593 | 0.0342 | 0.0049 | 0.0013 | 0.0043 | 0.0035 |
Cell Periphery | 0.0006 | 0.0009 | 0.0007 | 0.0008 | 0.0003 | 0.0004 | 0.0029 | 0.0004 | 0.0005 | 0.0006 | 0.0005 | 0.0002 | 0.0022 | 0.0002 | 0.0005 | 0.0001 | 0.0003 | 0.0003 |
Cytoplasm | 0.3723 | 0.8073 | 0.6457 | 0.6799 | 0.6404 | 0.4491 | 0.5244 | 0.6522 | 0.6486 | 0.2429 | 0.4057 | 0.521 | 0.3108 | 0.7493 | 0.6942 | 0.5728 | 0.7441 | 0.5388 |
Cytoplasmic Foci | 0.1593 | 0.0314 | 0.0546 | 0.072 | 0.0813 | 0.13 | 0.1256 | 0.0272 | 0.0621 | 0.2042 | 0.0972 | 0.1698 | 0.1279 | 0.0112 | 0.0395 | 0.1521 | 0.1244 | 0.1125 |
Eisosomes | 0.0004 | 0.0001 | 0.0005 | 0.0001 | 0 | 0.0001 | 0.0031 | 0.0007 | 0.0001 | 0.0007 | 0.0001 | 0 | 0.0011 | 0 | 0.0002 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0121 | 0.0057 | 0.0047 | 0.0039 | 0.0057 | 0.0055 | 0.0072 | 0.0023 | 0.0044 | 0.0039 | 0.0024 | 0.0043 | 0.0188 | 0.0023 | 0.0056 | 0.0055 | 0.0027 | 0.0065 |
Endosome | 0.1234 | 0.0064 | 0.0269 | 0.0622 | 0.0508 | 0.0426 | 0.0685 | 0.0113 | 0.0456 | 0.0858 | 0.0562 | 0.0231 | 0.1276 | 0.0022 | 0.0119 | 0.1183 | 0.0399 | 0.0315 |
Golgi | 0.0311 | 0.003 | 0.0179 | 0.0107 | 0.0071 | 0.0157 | 0.0114 | 0.0021 | 0.0137 | 0.0602 | 0.0165 | 0.0123 | 0.0542 | 0.0006 | 0.009 | 0.0231 | 0.0073 | 0.0152 |
Lipid Particles | 0.0181 | 0.0004 | 0.0075 | 0.0026 | 0.0006 | 0.0032 | 0.0046 | 0.0002 | 0.003 | 0.02 | 0.0008 | 0.0013 | 0.0504 | 0.0001 | 0.004 | 0.0381 | 0.0001 | 0.0009 |
Mitochondria | 0.013 | 0.0018 | 0.029 | 0.0129 | 0.008 | 0.0053 | 0.0049 | 0.0016 | 0.0043 | 0.0479 | 0.0571 | 0.018 | 0.0282 | 0.0002 | 0.0157 | 0.0058 | 0.0061 | 0.0097 |
None | 0.0844 | 0.0552 | 0.0881 | 0.0096 | 0.0069 | 0.029 | 0.1131 | 0.2066 | 0.0939 | 0.0205 | 0.0219 | 0.0373 | 0.0759 | 0.1916 | 0.1408 | 0.0248 | 0.0078 | 0.0539 |
Nuclear Periphery | 0.0219 | 0.0011 | 0.0016 | 0.0034 | 0.0038 | 0.0023 | 0.0071 | 0.0005 | 0.0011 | 0.002 | 0.0013 | 0.0013 | 0.0104 | 0.0004 | 0.0007 | 0.0021 | 0.0008 | 0.0009 |
Nucleolus | 0.0012 | 0.0002 | 0.0017 | 0.0002 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0.0001 | 0.0007 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Nucleus | 0.0072 | 0.0026 | 0.0028 | 0.0024 | 0.0085 | 0.0027 | 0.0233 | 0.0018 | 0.0013 | 0.0012 | 0.0023 | 0.0025 | 0.0028 | 0.0015 | 0.0013 | 0.001 | 0.0018 | 0.0017 |
Peroxisomes | 0.0143 | 0.004 | 0.0178 | 0.0054 | 0.0075 | 0.0104 | 0.0157 | 0.003 | 0.0073 | 0.0652 | 0.0175 | 0.0176 | 0.0241 | 0.0003 | 0.0133 | 0.0383 | 0.0057 | 0.0189 |
Punctate Nuclear | 0.0192 | 0.0011 | 0.0045 | 0.0007 | 0.0022 | 0.0025 | 0.0049 | 0.0013 | 0.0007 | 0.0019 | 0.0023 | 0.0039 | 0.0073 | 0.0006 | 0.0006 | 0.0048 | 0.0011 | 0.0017 |
Vacuole | 0.0092 | 0.0023 | 0.0031 | 0.0139 | 0.0054 | 0.0021 | 0.003 | 0.0022 | 0.0062 | 0.0069 | 0.005 | 0.0013 | 0.0044 | 0.0005 | 0.0014 | 0.0052 | 0.0064 | 0.0011 |
Vacuole Periphery | 0.0046 | 0.0003 | 0.0017 | 0.0048 | 0.0017 | 0.0008 | 0.0007 | 0.0004 | 0.0027 | 0.002 | 0.0017 | 0.0004 | 0.0018 | 0.0001 | 0.0004 | 0.0016 | 0.0014 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.2037 | 44.1063 | 96.6986 | 69.3112 | 15.9926 | 20.1731 | 72.2992 | 110.5916 | 76.4151 | 28.4966 |
Translational Efficiency | 1.0338 | 1.3925 | 1.0032 | 0.8065 | 0.9982 | 0.7094 | 1.0695 | 0.9575 | 0.9411 | 0.8349 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
675 | 1438 | 312 | 1431 | 1740 | 1628 | 127 | 451 | 2415 | 3066 | 439 | 1882 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 725.73 | 821.58 | 1110.66 | 1127.81 | 763.35 | 787.62 | 1200.35 | 1113.30 | 752.84 | 803.55 | 1136.61 | 1124.33 |
Standard Deviation | 425.99 | 98.34 | 104.50 | 175.49 | 89.39 | 97.23 | 124.38 | 165.85 | 238.25 | 99.21 | 117.86 | 173.34 |
Intensity Change Log 2 | — | 0.178968 | 0.613912 | 0.636019 | — | 0.045155 | 0.653039 | 0.544426 | — | 0.111922 | 0.634102 | 0.589792 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001211 | 0.001900 | 0.001030 | 0.010560 | 0.000647 | 0.001693 | 0.001085 | 0.002639 | 0.000805 | 0.001790 | 0.001046 | 0.008662 |
Bud Neck | 0.128781 | 0.079670 | 0.006880 | 0.019272 | 0.076027 | 0.099170 | 0.015345 | 0.021548 | 0.090772 | 0.090024 | 0.009329 | 0.019817 |
Bud Site | 0.159570* | 0.135810* | 0.026775 | 0.238546* | 0.087284 | 0.119989* | 0.051388 | 0.121025* | 0.107488* | 0.127409* | 0.033896 | 0.210383* |
Cell Periphery | 0.000654 | 0.000219 | 0.000184 | 0.000180 | 0.000167 | 0.000218 | 0.000120 | 0.000121 | 0.000303 | 0.000218 | 0.000166 | 0.000166 |
Cytoplasm | 0.326490* | 0.212454* | 0.488300* | 0.536968* | 0.361945* | 0.226857* | 0.519179* | 0.389844* | 0.352035* | 0.220102* | 0.497233* | 0.501711* |
Cytoplasmic Foci | 0.194988* | 0.237904* | 0.038064 | 0.053644 | 0.270386* | 0.233920* | 0.042657 | 0.084479 | 0.249312* | 0.235788* | 0.039393 | 0.061033 |
Eisosomes | 0.000206 | 0.000152 | 0.000058 | 0.000037 | 0.000116 | 0.000157 | 0.000044 | 0.000041 | 0.000141 | 0.000155 | 0.000054 | 0.000038 |
Endoplasmic Reticulum | 0.001726 | 0.000891 | 0.004992 | 0.000641 | 0.001020 | 0.000491 | 0.002078 | 0.001395 | 0.001218 | 0.000679 | 0.004149 | 0.000821 |
Endosome | 0.030469 | 0.071012 | 0.008213 | 0.005730 | 0.038707 | 0.073261 | 0.010533 | 0.033828 | 0.036405 | 0.072206 | 0.008884 | 0.012464 |
Golgi | 0.019281 | 0.035722 | 0.000457 | 0.005224 | 0.013739 | 0.031033 | 0.000571 | 0.003907 | 0.015288 | 0.033232 | 0.000490 | 0.004908 |
Lipid Particles | 0.004303 | 0.002987 | 0.002750 | 0.001177 | 0.005661 | 0.003500 | 0.001598 | 0.003509 | 0.005281 | 0.003260 | 0.002417 | 0.001736 |
Mitochondria | 0.034278 | 0.047424 | 0.000336 | 0.002298 | 0.016439 | 0.043221 | 0.000192 | 0.007056 | 0.021425 | 0.045192 | 0.000294 | 0.003438 |
Mitotic Spindle | 0.004695 | 0.017991 | 0.017026 | 0.018858 | 0.006007 | 0.019678 | 0.012997 | 0.060098 | 0.005640 | 0.018887 | 0.015860 | 0.028741 |
None | 0.005421 | 0.000676 | 0.002591 | 0.000738 | 0.001880 | 0.000686 | 0.003328 | 0.003381 | 0.002870 | 0.000681 | 0.002805 | 0.001372 |
Nuclear Periphery | 0.000081 | 0.000132 | 0.001272 | 0.000096 | 0.000158 | 0.000136 | 0.000749 | 0.000404 | 0.000137 | 0.000134 | 0.001120 | 0.000170 |
Nuclear Periphery Foci | 0.000273 | 0.000273 | 0.007706 | 0.000729 | 0.000402 | 0.000195 | 0.003860 | 0.002690 | 0.000366 | 0.000232 | 0.006593 | 0.001199 |
Nucleolus | 0.001123 | 0.001316 | 0.000470 | 0.000520 | 0.001424 | 0.001301 | 0.000421 | 0.000828 | 0.001340 | 0.001308 | 0.000456 | 0.000594 |
Nucleus | 0.028317 | 0.015421 | 0.214853* | 0.022017 | 0.033905 | 0.014091 | 0.084838 | 0.016521 | 0.032343 | 0.014715 | 0.177241* | 0.020700 |
Peroxisomes | 0.016560 | 0.043450 | 0.006042 | 0.020702 | 0.018807 | 0.054396 | 0.010498 | 0.026146 | 0.018179 | 0.049262 | 0.007331 | 0.022007 |
Vacuole | 0.036917 | 0.087101 | 0.171526 | 0.061100 | 0.061828 | 0.071593 | 0.237891* | 0.215647* | 0.054866 | 0.078866 | 0.190725 | 0.098135 |
Vacuole Periphery | 0.004654 | 0.007493 | 0.000475 | 0.000964 | 0.003449 | 0.004415 | 0.000627 | 0.004892 | 0.003786 | 0.005859 | 0.000519 | 0.001905 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.23 | 0.14 | -5.43 | -5.35 | -6.05 | -2.17 | -2.34 | -2.65 | -0.07 | -0.22 | -3.18 | -1.82 | -6.63 | -5.19 | -5.95 |
Bud Neck | 5.70 | 15.21 | 14.46 | 17.10 | -4.03 | -4.05 | 12.03 | 14.60 | 18.74 | 0.06 | 0.28 | 21.45 | 21.04 | 25.92 | -2.84 |
Bud Site | 2.59 | 15.31 | 1.19 | -1.70 | -18.42 | -5.49 | 6.53 | 1.72 | 5.64 | -3.93 | -3.83 | 16.75 | -3.76 | -0.68 | -18.09 |
Cell Periphery | 3.56 | 3.79 | 3.92 | 1.93 | 0.82 | -1.56 | 1.65 | 5.13 | 4.40 | 3.09 | 2.25 | 3.49 | 4.09 | 3.22 | 1.48 |
Cytoplasm | 8.84 | -4.61 | -14.98 | -31.56 | -6.92 | 14.65 | -0.06 | -1.95 | -10.47 | -1.05 | 17.91 | -3.29 | -15.90 | -33.50 | -6.74 |
Cytoplasmic Foci | -4.36 | 18.72 | 17.89 | 30.74 | -3.53 | 4.73 | 33.94 | 29.11 | 24.66 | -4.56 | 2.27 | 40.01 | 39.14 | 41.84 | -5.24 |
Eisosomes | 2.10 | 5.80 | 7.02 | 10.10 | 5.04 | -2.41 | 7.89 | 14.21 | 7.51 | 3.23 | -1.02 | 9.24 | 13.02 | 11.52 | 5.82 |
Endoplasmic Reticulum | 1.75 | -5.90 | 2.05 | 0.98 | 9.69 | 3.59 | -4.58 | 0.25 | -1.87 | 4.07 | 3.18 | -8.90 | 2.24 | -0.92 | 10.33 |
Endosome | -10.99 | 9.09 | 10.91 | 23.89 | 4.79 | -10.63 | 13.13 | 7.50 | 15.99 | -3.37 | -14.65 | 17.87 | 18.22 | 32.20 | 0.83 |
Golgi | -6.26 | 11.08 | 8.78 | 15.71 | -2.41 | -8.69 | 14.57 | 11.98 | 16.45 | -3.03 | -11.59 | 18.26 | 12.31 | 21.23 | -2.84 |
Lipid Particles | 2.01 | 2.01 | 4.03 | 3.25 | 3.52 | 3.00 | 6.06 | 4.21 | 1.37 | -0.19 | 4.05 | 5.65 | 7.52 | 3.37 | 2.78 |
Mitochondria | -2.73 | 9.46 | 9.08 | 15.35 | -1.33 | -8.75 | 11.44 | 6.08 | 12.57 | -1.43 | -9.57 | 14.66 | 12.59 | 20.87 | -1.95 |
Mitotic Spindle | -6.93 | -1.97 | -6.26 | 0.12 | -0.55 | -7.43 | -1.55 | -5.36 | -2.98 | -3.89 | -9.98 | -2.17 | -8.07 | -1.56 | -2.10 |
None | 3.21 | 1.99 | 3.06 | -1.86 | 5.28 | 4.61 | -0.17 | -0.26 | -2.18 | -0.10 | 4.86 | 1.15 | 3.59 | -2.82 | 3.79 |
Nuclear Periphery | -2.77 | -17.15 | -10.58 | -5.90 | 15.10 | 0.70 | -9.65 | -7.11 | -7.43 | 5.60 | -0.23 | -18.78 | -9.72 | -9.60 | 16.06 |
Nuclear Periphery Foci | -0.18 | -9.97 | -7.22 | -7.87 | 8.67 | 3.78 | -7.83 | -4.50 | -5.01 | 3.23 | 2.80 | -11.55 | -7.96 | -9.20 | 9.34 |
Nucleolus | -0.56 | 3.34 | 3.59 | 2.70 | 1.11 | 0.28 | 4.24 | 3.17 | 2.95 | -1.71 | 0.10 | 4.49 | 4.37 | 4.08 | 0.57 |
Nucleus | 3.79 | -16.89 | -5.07 | -12.54 | 15.59 | 10.24 | -6.64 | 2.21 | -5.73 | 7.08 | 10.44 | -17.53 | -4.52 | -14.55 | 16.39 |
Peroxisomes | -9.63 | 5.85 | 1.93 | 11.82 | -4.62 | -12.75 | 5.97 | 2.24 | 12.65 | -2.95 | -15.74 | 9.03 | 3.69 | 18.04 | -5.45 |
Vacuole | -11.50 | -14.79 | -20.45 | -11.28 | 5.43 | -3.20 | -13.13 | -19.12 | -18.21 | 1.84 | -8.87 | -18.24 | -25.12 | -20.34 | 5.88 |
Vacuole Periphery | -4.17 | 8.73 | 8.23 | 14.57 | -0.91 | -3.04 | 13.93 | 2.22 | 3.63 | -2.68 | -6.35 | 16.20 | 10.68 | 16.23 | -2.71 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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