Standard name
Human Ortholog
Description (SGD) Mitochondrial ribosomal protein of the large subunit; has similarity to E. coli L7/L12 and human MRPL7 ribosomal proteins; associates with the mitochondrial nucleoid; required for normal respiratory growth
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0.05 0.06 0 0 0 0 0 0.05
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0.14 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0.05 0.17 0 0 0 0 0 0
Golgi 0 0 0.2 0.14 0.37 0 0.06 – 0 0 0 0 0.08 0.05 0.09 – 0.53 0.39 0.05 0.1 0.05 0.05 0.1 0.07
Mitochondria 0.91 0.92 0.82 0.87 0.72 0.84 0.73 – 0.84 0.83 0.89 0.88 0.93 0.9 0.89 – 0.08 0.11 0.87 0.77 0.85 0.8 0.68 0.71
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0.06 0 0 0 0 0 0
Nuclear Periphery 0.07 0 0 0 0 0.07 0 – 0.07 0.05 0.05 0 0.05 0 0 – 0 0.06 0 0 0 0 0 0
Nucleolus 0.11 0.09 0.09 0 0 0.22 0.18 – 0.16 0.18 0.12 0.13 0.09 0.09 0.05 – 0.14 0.28 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.06 0 0 – 0 0 0 0 0 0 0.05 – 0 0 0 0 0.05 0.05 0.1 0.09
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0.12 0.09 0.06 0.12 0 0.11 – 0 0 0 0 0.08 0.1 0.09 – 0.17 0.11 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 4 5 0 2 – 2 2 2 4 1 2 0 – 3 1 2 4 1 4 16 15
Bud 0 0 0 1 0 3 3 – 3 1 5 3 2 2 1 – 1 0 0 0 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 2
Bud Site 0 0 0 0 0 1 0 – 1 0 1 1 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 2 0 2 3 0 2 – 0 0 0 1 2 3 1 – 0 0 0 0 0 0 0 0
Cytoplasm 0 1 0 5 6 5 11 – 15 11 9 9 3 11 5 – 8 0 0 0 0 0 0 0
Endoplasmic Reticulum 2 3 0 1 1 3 1 – 1 3 2 0 1 0 1 – 1 0 0 0 0 0 0 0
Endosome 1 1 2 1 3 0 5 – 1 1 3 1 1 5 8 – 3 3 0 2 0 3 7 5
Golgi 1 4 9 26 81 1 25 – 11 16 7 16 13 18 41 – 31 7 6 14 4 9 38 23
Mitochondria 127 134 37 167 159 165 292 – 296 336 250 332 151 333 399 – 5 2 127 116 63 150 269 224
Nucleus 2 0 1 0 0 1 1 – 1 1 2 4 0 0 0 – 0 1 0 0 0 0 0 0
Nuclear Periphery 10 1 1 5 1 14 18 – 26 21 13 15 8 13 10 – 1 1 0 0 0 0 2 2
Nucleolus 16 13 4 7 7 43 71 – 58 73 33 50 15 34 23 – 8 5 1 0 0 3 9 2
Peroxisomes 2 1 1 2 14 1 7 – 3 1 1 6 4 7 23 – 0 0 4 3 3 9 40 28
SpindlePole 0 0 0 1 0 0 3 – 2 0 0 0 0 0 0 – 2 0 0 1 0 1 1 0
Vac/Vac Membrane 10 17 4 11 27 6 44 – 14 18 5 9 13 37 40 – 10 2 1 5 0 1 5 6
Unique Cell Count 140 145 45 192 220 197 400 352 404 282 377 162 372 449 59 18 146 151 74 188 399 317
Labelled Cell Count 171 177 61 233 307 243 485 434 484 333 451 214 465 552 73 22 146 151 74 188 399 317


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.1 16.3 14.0 15.7 18.8 13.2 19.9 – 18.7 20.0 17.9 18.9 19.7 20.0 19.5 – 27.3 24.3 18.1 19.2 19.3
Std Deviation (1e-4) 3.9 4.6 4.7 3.6 4.5 4.3 5.2 – 5.4 5.9 5.5 5.9 5.9 5.5 4.2 – 10.1 9.1 4.8 5.9 5.2
Intensity Change (Log2) – – – 0.16 0.42 -0.09 0.51 – 0.42 0.52 0.36 0.44 0.5 0.51 0.48 – 0.96 0.8 0.37 0.46 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Cytoplasm 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Golgi -1.1 2.2 0 0 – 0 0 0 0 0 0 0 – 3.4 0
Mitochondria 0.8 -1.4 0.2 -1.3 – 0.3 0.2 1.2 1.1 2.3 1.5 1.3 – -7.6 -5.3
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Nucleolus 0 0 2.0 1.5 – 1.3 1.5 0.6 0.8 0 0 0 – 0.7 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Vacuole 0 0.6 0 0 – 0 0 0 0 0 0 0 – 1.2 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 18.9941 20.5998 19.132 16.4876 15.5653 17.9036 25.0291 26.1948 25.5771 25.0788 18.9082 25.0823 23.4003 25.6781 26.3441 24.9871 23.6677 25.2349
Actin 0.0197 0.0139 0.0178 0.012 0.0006 0.0175 0.052 0.0012 0.0061 0.0363 0.0014 0.0026 0.0567 0.0018 0.0159 0.0001 0.0005 0.0003
Bud 0.0002 0.0001 0.0001 0.0002 0.0066 0.0005 0.0006 0 0.0012 0.0006 0.0005 0.0001 0.0002 0 0.0002 0 0 0.0001
Bud Neck 0.0013 0 0.0036 0.0007 0.0006 0.0006 0.0021 0 0.0006 0.0003 0.0002 0.0001 0.0007 0 0.0001 0 0 0
Bud Periphery 0.0002 0.0001 0.0002 0.0002 0.009 0.0019 0.0011 0.0001 0.0016 0.0009 0.0008 0.0002 0.0004 0 0.0004 0 0 0.0001
Bud Site 0.0017 0.0003 0.0005 0.0036 0.0112 0.0005 0.0028 0.0001 0.0048 0.0023 0.0007 0.0001 0.0009 0 0.0033 0 0 0.0001
Cell Periphery 0.0001 0.0001 0.0001 0.0001 0.0005 0.0001 0.0005 0.0002 0.0003 0.0005 0.0002 0.0001 0.0003 0.0001 0.0004 0.0001 0 0.0001
Cytoplasm 0.0169 0 0.0004 0.0063 0.0011 0.0007 0.0008 0.0001 0.0042 0.0001 0.0002 0.0001 0.0029 0 0.0002 0 0 0.0001
Cytoplasmic Foci 0.017 0.0005 0.0037 0.0193 0.0055 0.0118 0.0158 0.0042 0.0102 0.0024 0.0085 0.0002 0.0325 0.0001 0.0025 0 0 0.0005
Eisosomes 0.0004 0.0004 0.0005 0.0004 0.0001 0.0002 0.0007 0.0004 0.0004 0.0009 0.0003 0.0004 0.0016 0.0002 0.0006 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0028 0 0.0003 0.001 0.0005 0.0017 0.0023 0.0001 0.0001 0.0002 0.0004 0.0004 0.0039 0 0.0004 0 0 0.0002
Endosome 0.0106 0.0009 0.0038 0.0052 0.0172 0.012 0.0079 0.0016 0.0023 0.004 0.042 0.0018 0.0123 0.0005 0.0006 0.0002 0 0.0019
Golgi 0.008 0.0038 0.0114 0.0025 0.0038 0.0122 0.0084 0.0092 0.0031 0.0103 0.016 0.0027 0.0041 0.0033 0.0016 0.0001 0.0001 0.0023
Lipid Particles 0.0158 0.0137 0.0041 0.0036 0.0093 0.0356 0.0237 0.0065 0.0087 0.0054 0.0402 0.0087 0.0179 0.0109 0.0138 0.0012 0.0002 0.0024
Mitochondria 0.8682 0.9409 0.9353 0.9208 0.8494 0.8256 0.8236 0.9564 0.9147 0.9157 0.8638 0.9456 0.7843 0.9649 0.9346 0.9684 0.9979 0.9762
None 0.0023 0 0.0002 0.0071 0.0004 0.0009 0.0018 0.0005 0.0004 0.0001 0.0001 0.0002 0.0143 0 0.0002 0 0 0.0002
Nuclear Periphery 0.0125 0.0001 0.0067 0.0008 0.0022 0.0367 0.013 0.0003 0.0001 0.0003 0.0005 0.0244 0.0036 0.0001 0.0001 0.0001 0 0.001
Nucleolus 0.0003 0.0001 0.0001 0.0003 0.0025 0.0004 0.0167 0.0004 0.0007 0.0003 0.0006 0.0008 0.001 0.0001 0.0003 0.0001 0 0.0015
Nucleus 0.0044 0 0.0011 0.0006 0.0025 0.0031 0.0035 0.0002 0.0003 0.0002 0.0004 0.0035 0.0007 0 0.0001 0 0 0.0018
Peroxisomes 0.0065 0.0064 0.0056 0.0081 0.0022 0.006 0.0061 0.0031 0.0069 0.0032 0.008 0.0005 0.0102 0.0024 0.0221 0.0001 0.001 0.0033
Punctate Nuclear 0.0044 0 0.0003 0.0067 0.0009 0.0048 0.0092 0.001 0.0004 0.0001 0.0001 0.0002 0.016 0 0.0001 0 0 0.0001
Vacuole 0.0013 0.0034 0.0005 0.0003 0.0215 0.0014 0.003 0.002 0.0106 0.0042 0.0073 0.0008 0.0013 0.001 0.0005 0.0026 0 0.0021
Vacuole Periphery 0.0054 0.0153 0.0037 0.0003 0.0525 0.0257 0.0045 0.0124 0.0222 0.0122 0.0079 0.0067 0.0341 0.0144 0.002 0.0269 0.0001 0.0056

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 90.5357 69.1306 66.3119 67.6125 85.5922 120.6475 107.8303 73.0659 109.0137 89.6483
Translational Efficiency 1.7327 2.373 2.3219 2.624 1.8394 1.6013 1.9411 3.7071 2.094 2.3217

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘


Micrographs

Cell Count

Number of cells analysed for each strain. R1, R2: biological replicates; R1 & R2: both replicates combined.

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
270 545 88 1565 1488 2026 1978 1628 1758 2571 2066 3193

Protein Abundance

Mean and standard deviation of cell GFP intensity, and intensity change (log2) of each mutant relative to wild type.

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1121.09 1683.82 2155.79 2155.82 1393.44 1796.27 2227.46 2447.66 1351.61 1772.43 2224.41 2304.62
Standard Deviation 235.63 397.48 434.76 517.80 290.29 382.64 498.75 566.18 299.16 388.56 496.40 562.26
Intensity Change Log 2 — 0.586836 0.943315 0.943335 — 0.366353 0.676749 0.812752 — 0.468836 0.801712 0.872436

Localization Score (DeepLoc)

Localization score for each compartment. Values highlighted in green denote significant localization scores (marked * in the plot).

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002346 0.013225 0.024200 0.008717 0.001675 0.002274 0.009604 0.013152 0.001778 0.004595 0.010226 0.010978
Bud Neck 0.002113 0.002893 0.015101 0.001815 0.001154 0.000417 0.001639 0.001279 0.001301 0.000941 0.002212 0.001542
Bud Site 0.001874 0.002858 0.005216 0.003489 0.001298 0.000202 0.001278 0.001877 0.001387 0.000765 0.001446 0.002667
Cell Periphery 0.000447 0.000334 0.000254 0.000486 0.000081 0.000035 0.000184 0.000301 0.000137 0.000098 0.000187 0.000392
Cytoplasm 0.000426 0.000558 0.000509 0.000320 0.000138 0.000079 0.000254 0.000414 0.000183 0.000180 0.000264 0.000368
Cytoplasmic Foci 0.023533 0.027780 0.096316 0.006907 0.014539 0.007324 0.014233 0.008291 0.015920 0.011660 0.017730 0.007613
Eisosomes 0.000412 0.002598 0.001672 0.001797 0.000757 0.000927 0.001130 0.002124 0.000704 0.001281 0.001153 0.001964
Endoplasmic Reticulum 0.000370 0.000330 0.000466 0.000324 0.000118 0.000048 0.000258 0.000175 0.000156 0.000108 0.000267 0.000248
Endosome 0.007258 0.004432 0.005670 0.001521 0.001554 0.000830 0.002173 0.001884 0.002430 0.001593 0.002322 0.001706
Golgi 0.026273 0.050654 0.039769 0.016325 0.016179 0.021780 0.032347 0.023064 0.017729 0.027901 0.032663 0.019761
Lipid Particles 0.009378 0.011357 0.044386 0.003501 0.009598 0.005404 0.009560 0.003429 0.009564 0.006666 0.011044 0.003464
Mitochondria 0.879316* 0.841954* 0.602827* 0.925476* 0.921011* 0.947648* 0.877527* 0.917222* 0.914608* 0.925243* 0.865827* 0.921268*
Mitotic Spindle 0.006661 0.000612 0.026243 0.000940 0.000664 0.000089 0.000796 0.001500 0.001585 0.000200 0.001880 0.001225
None 0.002029 0.002964 0.003697 0.002720 0.003653 0.002688 0.003274 0.002799 0.003404 0.002747 0.003292 0.002761
Nuclear Periphery 0.002383 0.000346 0.009762 0.000820 0.000875 0.000197 0.000852 0.000509 0.001107 0.000229 0.001231 0.000661
Nuclear Periphery Foci 0.002011 0.000317 0.003410 0.000265 0.000895 0.000154 0.000333 0.000189 0.001067 0.000189 0.000464 0.000226
Nucleolus 0.000747 0.001140 0.004374 0.000583 0.000958 0.000145 0.000683 0.000325 0.000926 0.000356 0.000841 0.000451
Nucleus 0.000236 0.000142 0.000337 0.000088 0.000147 0.000020 0.000059 0.000048 0.000160 0.000046 0.000070 0.000068
Peroxisomes 0.022142 0.017054 0.019438 0.003350 0.006838 0.003328 0.004774 0.005773 0.009188 0.006237 0.005399 0.004586
Vacuole 0.004841 0.010203 0.042470 0.006609 0.007226 0.002143 0.011305 0.004006 0.006860 0.003852 0.012632 0.005282
Vacuole Periphery 0.005204 0.008250 0.053885 0.013948 0.010641 0.004268 0.027736 0.011636 0.009806 0.005112 0.028849 0.012769

Localization Changes (T-score)

Change in localization of each mutant relative to its control (e.g. UBP2_WT: ubp2Δ vs wild type). Values highlighted in yellow (increase) or blue (decrease) denote significant changes (marked * in the plot).

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.90 -2.15 -5.18 1.82 1.51 -1.11 -7.04 -7.37 -6.97 -1.92 -4.36 -7.33 -9.09 -5.75 -0.52
Bud Neck -1.06 -4.01 0.50 1.61 4.12 1.74 -1.11 -0.31 -3.60 1.30 0.93 -2.22 -0.66 -2.48 2.23
Bud Site -0.95 -2.48 -1.87 -0.57 1.16 1.97 0.03 -0.88 -3.58 -1.27 1.19 -0.13 -2.08 -4.04 -2.65
Cell Periphery 0.37 0.72 -0.15 -0.85 -2.18 2.33 -4.00 -3.63 -4.59 -1.96 0.70 -1.08 -3.59 -4.50 -3.49
Cytoplasm -0.66 -0.58 0.99 1.20 1.39 3.07 -3.36 -1.87 -2.23 -1.18 0.05 -2.37 -2.25 -2.13 -1.33
Cytoplasmic Foci -0.87 -4.33 4.26 6.20 5.43 5.25 0.17 4.21 -1.08 4.32 2.89 -1.04 6.16 3.84 6.95
Eisosomes -8.61 -3.62 -6.91 2.55 -0.32 -1.66 -4.00 -4.36 -3.76 -3.15 -5.81 -5.18 -6.62 -3.38 -4.14
Endoplasmic Reticulum 0.37 -0.54 0.39 0.05 0.83 3.23 -2.40 -1.44 -3.70 1.29 1.83 -1.92 -2.01 -3.31 0.28
Endosome 1.73 0.81 3.92 3.58 3.20 2.75 -2.03 -0.74 -2.52 0.63 2.35 0.28 1.88 -0.41 1.95
Golgi -3.43 -1.40 1.92 6.20 2.75 -2.23 -5.80 -2.65 -0.49 3.18 -4.15 -5.56 -0.97 3.60 5.14
Lipid Particles -0.66 -2.69 2.60 3.68 3.18 2.93 0.02 4.54 2.19 5.47 2.21 -0.96 5.18 3.90 6.49
Mitochondria 2.07 7.11 -3.02 -7.30 -8.87 -4.74 6.29 0.62 5.52 -5.73 -1.87 7.02 -1.13 0.92 -8.96
Mitotic Spindle 1.82 -1.64 1.72 -0.99 2.24 2.15 -0.41 -1.30 -2.35 -1.15 2.45 -0.34 0.55 -3.08 1.01
None -1.95 -2.64 -1.57 0.95 2.01 3.42 1.39 3.01 -0.57 2.61 2.64 0.45 2.70 -0.09 3.61
Nuclear Periphery 1.52 -2.22 1.15 -1.63 2.92 2.74 0.08 1.28 -2.11 1.80 2.99 -0.36 1.34 -2.74 2.40
Nuclear Periphery Foci 1.77 -1.10 1.82 0.52 3.79 2.40 1.88 2.35 -0.39 2.66 2.94 2.04 2.85 -0.51 3.67
Nucleolus -0.90 -2.30 0.44 1.92 2.45 3.19 1.05 2.48 -3.65 4.08 2.48 0.34 2.09 -1.18 3.39
Nucleus 1.09 -0.88 1.84 1.25 2.98 2.98 2.08 2.26 -3.22 0.95 2.96 2.37 2.35 -1.74 0.09
Peroxisomes 0.89 0.32 3.76 4.82 2.36 2.69 1.50 0.68 -2.04 -0.79 2.01 2.58 3.34 1.69 0.83
Vacuole -2.28 -3.99 -1.31 1.58 3.81 5.46 -3.08 3.18 -2.81 6.28 3.30 -4.46 1.73 -2.00 6.21
Vacuole Periphery -1.56 -4.46 -4.79 -2.83 3.65 4.84 -7.30 -0.57 -5.56 6.86 4.02 -8.53 -2.12 -7.42 7.42
Standard name
Human Ortholog
Description (SGD) Mitochondrial ribosomal protein of the large subunit; has similarity to E. coli L7/L12 and human MRPL7 ribosomal proteins; associates with the mitochondrial nucleoid; required for normal respiratory growth
Localization
Cell Percentages mitochondrion (98%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Mnp1

Mnp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)