Standard name
Human Ortholog
Description Conserved nuclear protein that interacts with GTP-Gsp1p; stimulates nucleotide release from Gsp1p; involved in nuclear protein import; nucleotide release is inhibited by Yrb1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.96 0.96 0.88 0.89 0.85 0.72 – 0.73 0.67 0.6 0.59 0.98 0.98 0.98 0.93 0.9 0.89 0.91 0.86 0.9 0.92 0.9 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.2 0.09 – 0.29 0.26 0.34 0.36 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0.06 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.09 0.16 0.14 0.08 0.29 – 0.13 0.14 0.19 0.12 0 0 0 0.17 0.16 0.17 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 1
Bud 0 1 1 1 0 5 2 – 1 2 1 12 0 0 0 0 0 0 1 10 5 3 8 8
Bud Neck 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0 4 1 1 1 3
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 3 2 1 – 2 3 4 1 0 0 3 1 2 1 0 0 0 0 0 0
Cytoplasm 77 459 395 284 308 282 410 – 256 215 172 207 298 443 412 158 214 195 76 419 383 244 321 315
Endoplasmic Reticulum 1 4 1 0 1 0 0 – 1 3 0 1 1 0 1 10 5 9 0 0 3 2 1 1
Endosome 0 0 2 0 5 0 4 – 0 1 1 3 0 0 1 0 1 0 1 2 3 0 2 4
Golgi 0 1 0 0 0 0 1 – 0 0 0 0 2 1 0 0 2 0 0 4 0 0 2 1
Mitochondria 1 8 1 9 6 67 53 – 103 82 99 127 0 0 2 2 4 1 0 5 4 2 1 1
Nucleus 0 9 1 2 6 6 12 – 12 19 13 27 0 2 0 7 3 6 0 19 5 2 1 1
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 2 1 0 2 1 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 – 0 0 0 3 0 0 0 0 0 0 0 6 4 2 3 3
Vac/Vac Membrane 1 16 39 53 48 25 162 – 45 45 56 42 6 8 4 29 39 38 0 9 2 1 0 4
Unique Cell Count 80 477 412 323 348 331 568 352 320 288 353 304 453 419 170 237 218 84 489 425 266 355 351
Labelled Cell Count 80 498 440 350 377 388 646 420 370 348 427 307 456 424 207 270 250 84 489 425 266 355 351


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 5.8 6.6 6.4 6.9 4.8 6.1 – 5.4 5.6 5.7 5.3 7.0 7.3 7.0 10.4 11.0 10.6 5.6 6.0 6.2
Std Deviation (1e-4) 1.2 1.1 1.6 1.4 1.5 1.2 1.4 – 1.2 1.2 2.5 1.2 1.7 1.7 1.5 2.3 2.2 2.3 1.4 1.6 1.5
Intensity Change (Log2) – – – -0.04 0.07 -0.45 -0.12 – -0.29 -0.24 -0.21 -0.32 0.08 0.16 0.09 0.66 0.74 0.68 -0.23 -0.13 -0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 3.4 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -4.0 -3.8 -5.1 -9.6 – -9.0 -10.3 -12.0 -12.5 1.6 1.6 2.1 -1.5 -2.8 -3.1
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 6.2 – 11.7 10.7 12.7 13.2 0 0 0 0 0 0
Nucleus 0 0 0 2.5 – 3.4 4.7 4.0 5.4 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 2.8 1.9 -0.9 7.3 – 1.5 1.9 3.8 1.1 -4.1 -5.0 -5.5 2.6 2.6 2.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0233 0.0009 0.0043 0.0372 0.0037 0.0107 0.0196 0.0005 0.0156 0.0391 0.0192 0.0017 – – – – – –
Bud 0.0005 0.0013 0.0003 0.0018 0.001 0.0004 0.0009 0.0002 0.002 0.0006 0.0028 0.0003 – – – – – –
Bud Neck 0.0064 0.0004 0.0007 0.0009 0.0011 0.0137 0.0009 0.0039 0.005 0.0015 0.0158 0.0012 – – – – – –
Bud Periphery 0.0005 0.0002 0.0002 0.0023 0.0011 0.0006 0.0007 0 0.0013 0.0008 0.0031 0.0001 – – – – – –
Bud Site 0.0062 0.0021 0.001 0.0022 0.0009 0.0003 0.0026 0.0021 0.0041 0.0019 0.0506 0.0002 – – – – – –
Cell Periphery 0.0003 0.0001 0.0001 0.0001 0 0.0001 0.0001 0 0.0003 0.0001 0.0005 0 – – – – – –
Cytoplasm 0.6402 0.8453 0.8597 0.7045 0.779 0.8295 0.7618 0.9073 0.7825 0.8226 0.8037 0.87 – – – – – –
Cytoplasmic Foci 0.0319 0.0095 0.0069 0.0202 0.0177 0.0169 0.0136 0.0067 0.0126 0.017 0.0084 0.0105 – – – – – –
Eisosomes 0.0003 0 0 0.0002 0 0 0.0001 0 0.0003 0.0002 0.0001 0 – – – – – –
Endoplasmic Reticulum 0.0078 0.0053 0.0045 0.0031 0.0034 0.0046 0.0049 0.0019 0.0031 0.0086 0.0061 0.0045 – – – – – –
Endosome 0.034 0.0049 0.0066 0.0191 0.018 0.0123 0.0132 0.0014 0.0126 0.0102 0.0125 0.0052 – – – – – –
Golgi 0.015 0.0008 0.0023 0.0075 0.0042 0.0015 0.0048 0.0003 0.0053 0.0059 0.0018 0.001 – – – – – –
Lipid Particles 0.0255 0.0011 0.0013 0.0211 0.0011 0.0027 0.0051 0.0001 0.0031 0.0044 0.0008 0.0002 – – – – – –
Mitochondria 0.0157 0.0006 0.0019 0.0044 0.0116 0.0011 0.0041 0.0002 0.0108 0.0022 0.0013 0.0015 – – – – – –
None 0.0861 0.0616 0.0448 0.0883 0.0607 0.0247 0.0673 0.0268 0.0356 0.0301 0.0176 0.0169 – – – – – –
Nuclear Periphery 0.0146 0.0061 0.0055 0.0053 0.0154 0.0089 0.0145 0.0027 0.0059 0.0097 0.0203 0.0088 – – – – – –
Nucleolus 0.0071 0.0004 0.0001 0.0012 0.0007 0.0003 0.0005 0.0001 0.0015 0.0002 0.0005 0.0005 – – – – – –
Nucleus 0.051 0.0425 0.052 0.0327 0.0404 0.0577 0.0688 0.043 0.086 0.0307 0.0253 0.0646 – – – – – –
Peroxisomes 0.0111 0.0089 0.0035 0.0262 0.0168 0.0016 0.0072 0.0007 0.0048 0.0079 0.0011 0.0024 – – – – – –
Punctate Nuclear 0.0183 0.0064 0.0028 0.02 0.0197 0.01 0.0063 0.0016 0.0061 0.0046 0.0059 0.0095 – – – – – –
Vacuole 0.0029 0.0013 0.0011 0.0014 0.0027 0.002 0.0021 0.0007 0.0011 0.001 0.002 0.0007 – – – – – –
Vacuole Periphery 0.0013 0.0003 0.0003 0.0005 0.0007 0.0005 0.0009 0.0001 0.0005 0.0007 0.0008 0.0003 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 57.9413 63.056 49.0184 47.3023 45.7273 58.7485 57.3044 53.2764 59.8981 52.6802
Translational Efficiency 1.6018 1.1525 1.5324 1.4773 1.8815 1.744 1.3133 1.4682 1.4928 1.5431

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
97 1250 584 1980 1470 1991 2128 1738 1567 3241 2712 3718

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 706.62 841.95 1025.09 924.62 711.30 856.31 985.54 1019.68 711.01 850.77 994.06 969.06
Standard Deviation 97.16 128.45 146.19 154.30 102.93 115.52 145.23 151.61 102.59 120.88 146.34 160.23
Intensity Change Log 2 — 0.252800 0.536744 0.387926 — 0.267675 0.470456 0.519586 — 0.260281 0.503871 0.455475

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000482 0.000332 0.000519 0.000294 0.000110 0.000101 0.000329 0.000752 0.000133 0.000190 0.000370 0.000508
Bud Neck 0.026697 0.011771 0.003079 0.003487 0.005191 0.014274 0.003356 0.007899 0.006522 0.013309 0.003296 0.005550
Bud Site 0.062237 0.011184 0.004901 0.017828 0.003483 0.013485 0.003827 0.022054 0.007120 0.012598 0.004058 0.019804
Cell Periphery 0.000338 0.000117 0.000123 0.000113 0.000131 0.000078 0.000084 0.000110 0.000144 0.000093 0.000092 0.000112
Cytoplasm 0.338449* 0.622021* 0.284834* 0.689635* 0.580525* 0.649790* 0.522303* 0.581890* 0.565540* 0.639080* 0.471167* 0.639269*
Cytoplasmic Foci 0.252077* 0.105302 0.003101 0.010702 0.120333 0.062094 0.003082 0.018368 0.128488 0.078759 0.003086 0.014286
Eisosomes 0.000198 0.000043 0.000028 0.000017 0.000058 0.000028 0.000012 0.000029 0.000067 0.000034 0.000016 0.000023
Endoplasmic Reticulum 0.002989 0.001625 0.005229 0.000877 0.000840 0.000360 0.001779 0.001215 0.000973 0.000848 0.002522 0.001035
Endosome 0.012552 0.008950 0.001181 0.003118 0.004478 0.004465 0.000765 0.006943 0.004978 0.006195 0.000854 0.004906
Golgi 0.001592 0.002061 0.000098 0.001949 0.001080 0.000979 0.000086 0.001809 0.001111 0.001397 0.000088 0.001884
Lipid Particles 0.008826 0.002676 0.000793 0.000470 0.003373 0.000917 0.000430 0.000733 0.003710 0.001596 0.000508 0.000593
Mitochondria 0.004979 0.003058 0.000639 0.000907 0.002403 0.002319 0.000604 0.002191 0.002562 0.002604 0.000611 0.001507
Mitotic Spindle 0.003488 0.008663 0.004114 0.028931 0.000652 0.011959 0.008812 0.027296 0.000827 0.010688 0.007800 0.028167
None 0.002434 0.006080 0.006431 0.011731 0.007498 0.004984 0.009474 0.007954 0.007185 0.005407 0.008819 0.009965
Nuclear Periphery 0.000387 0.000371 0.001444 0.000611 0.000413 0.000362 0.001148 0.000896 0.000411 0.000365 0.001212 0.000744
Nuclear Periphery Foci 0.000855 0.001143 0.001242 0.001360 0.000811 0.000156 0.001345 0.001058 0.000814 0.000537 0.001323 0.001219
Nucleolus 0.001312 0.001034 0.000300 0.000282 0.000955 0.000877 0.000212 0.000723 0.000977 0.000938 0.000231 0.000488
Nucleus 0.211278* 0.150190* 0.628577* 0.145183* 0.232048* 0.182220* 0.403857* 0.193562* 0.230762* 0.169866* 0.452248* 0.167798*
Peroxisomes 0.009601 0.003142 0.000268 0.000354 0.001833 0.002162 0.000320 0.000804 0.002314 0.002540 0.000309 0.000565
Vacuole 0.057364 0.058837 0.052978 0.080143 0.033184 0.047681 0.038088 0.121140 0.034681 0.051984 0.041294 0.099308
Vacuole Periphery 0.001866 0.001400 0.000121 0.002005 0.000603 0.000708 0.000088 0.002573 0.000681 0.000975 0.000095 0.002271

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.93 0.16 1.05 1.26 3.99 0.29 -5.61 -1.98 -2.06 -0.17 -1.11 -5.34 -2.42 -2.05 1.38
Bud Neck 2.00 3.15 3.23 5.11 1.33 -6.46 1.57 0.27 6.69 -1.30 -5.62 2.98 2.71 8.75 -0.55
Bud Site 2.99 3.33 2.99 0.09 -3.19 -6.06 -0.15 -4.37 1.81 -4.65 -3.33 2.19 -2.20 1.56 -6.64
Cell Periphery 5.82 4.91 6.57 3.23 5.66 4.19 3.47 4.05 -0.68 1.66 4.22 3.56 4.85 1.19 3.51
Cytoplasm -11.91 4.50 -8.98 7.18 -29.25 -7.50 19.06 14.14 22.47 -3.88 -8.60 23.08 8.26 20.52 -18.30
Cytoplasmic Foci 6.58 11.35 11.11 23.10 -8.92 12.54 30.15 28.15 20.12 -8.96 10.91 31.57 30.00 30.36 -13.34
Eisosomes 6.80 6.85 7.65 7.45 5.69 11.29 16.10 10.35 -2.62 -10.36 11.05 14.94 13.94 4.00 -3.40
Endoplasmic Reticulum 1.65 -4.44 2.33 1.98 10.02 5.99 -12.17 -7.66 -15.42 5.88 0.80 -14.16 -4.98 -5.93 12.31
Endosome 1.24 4.05 4.05 8.49 0.10 0.39 8.86 3.45 3.16 -4.06 -1.71 9.38 6.11 8.69 -4.43
Golgi -0.82 3.87 1.87 2.79 -2.04 0.29 3.07 1.36 1.53 -1.96 -0.75 3.33 1.37 2.93 -2.86
Lipid Particles 3.07 4.00 4.27 3.02 4.72 7.15 7.93 7.36 0.32 -4.72 5.06 8.54 8.58 3.12 0.22
Mitochondria 1.08 2.50 2.51 3.77 0.15 0.08 2.17 1.64 2.95 -1.24 -0.05 2.50 2.26 5.07 -1.14
Mitotic Spindle -2.29 -0.38 -6.73 -4.39 -7.14 -5.90 -6.20 -9.15 -3.29 -5.22 -7.25 -6.52 -13.32 -5.13 -7.69
None -3.20 -3.40 -7.55 -2.73 -5.33 2.50 -0.64 -0.69 -4.91 -0.20 1.89 -0.29 -1.49 -5.51 -2.54
Nuclear Periphery -0.15 -16.66 -14.96 -20.50 7.87 0.86 -22.70 -19.22 -18.80 -1.06 0.82 -27.06 -24.83 -26.00 4.35
Nuclear Periphery Foci -1.92 -4.69 -2.53 -0.74 3.04 5.49 -5.16 -2.64 -12.84 3.38 2.10 -6.11 -3.40 -8.66 3.88
Nucleolus 0.78 3.73 3.64 5.61 -0.66 0.27 5.75 3.07 2.85 -2.24 0.07 6.15 4.63 5.74 -2.72
Nucleus 2.79 -17.82 -0.57 -10.73 35.36 6.75 -29.97 -6.84 -14.27 23.36 9.40 -36.79 -3.39 -16.48 40.24
Peroxisomes 3.00 4.31 4.33 6.98 0.57 -0.61 3.73 3.49 6.86 -1.21 -0.38 4.89 4.81 9.87 -0.71
Vacuole -0.78 -3.90 -11.39 -17.59 -8.47 -5.57 -13.08 -27.95 -24.52 -17.79 -7.94 -14.87 -35.51 -30.32 -20.22
Vacuole Periphery 0.79 3.03 1.83 2.07 -2.53 -0.59 3.89 -0.55 -0.21 -2.74 -1.70 4.49 -0.29 1.11 -3.82
Standard name
Human Ortholog
Description Conserved nuclear protein that interacts with GTP-Gsp1p; stimulates nucleotide release from Gsp1p; involved in nuclear protein import; nucleotide release is inhibited by Yrb1p
Localization
Cell Percentages cytoplasm (70%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Mog1

Mog1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mog1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available