Standard name
Human Ortholog
Description Mitochondrial intermembrane space protein of unknown function
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.97 0.98 0.96 0.94 0.81 0.78 – 0.59 0.56 0.58 0.55 0.98 0.97 0.99 0.9 0.84 0.89 0.92 0.88 0.78 0.92 0.82 0.68
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0.08 0 0 0 0 0 0 0.06
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.07 0.12 0.14 – 0.51 0.48 0.44 0.48 0 0 0 0.06 0 0 0 0 0.11 0 0.07 0.09
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.06 0.08 – 0 0.06 0.11 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 1 4 1 – 2 0 0 2 0 0 0 0 3 1 1 5 5 0 1 5
Bud 0 0 0 1 4 3 3 – 11 9 9 11 1 0 0 0 2 0 1 7 3 1 3 5
Bud Neck 0 1 3 1 2 1 2 – 0 0 1 0 0 0 0 0 0 0 0 5 2 1 2 2
Bud Site 0 0 0 0 0 0 1 – 0 0 2 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 3 7 6 2 5 3 – 5 4 4 5 4 8 3 8 15 7 1 1 2 0 0 1
Cytoplasm 299 618 321 234 234 295 318 – 227 184 199 168 293 334 409 300 286 280 307 563 271 305 216 230
Endoplasmic Reticulum 1 5 0 1 1 10 5 – 10 1 3 6 3 2 0 12 13 20 0 2 2 1 1 3
Endosome 1 1 1 0 2 15 12 – 7 7 12 6 2 1 1 15 27 14 3 7 3 2 5 21
Golgi 2 0 2 0 0 0 0 – 0 0 0 0 0 0 1 6 4 1 1 1 1 0 1 3
Mitochondria 2 16 1 9 18 43 55 – 194 158 150 145 0 0 0 19 14 4 7 25 38 10 18 31
Nucleus 0 1 1 0 0 4 3 – 1 5 4 2 0 1 0 2 2 2 0 0 0 2 0 2
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 1 1 1 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 1 4 6 0 0 0 0 0 0
Peroxisomes 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1
SpindlePole 0 1 2 2 0 2 7 – 5 4 1 1 0 3 0 4 15 11 1 5 5 2 4 14
Vac/Vac Membrane 5 6 1 3 5 20 34 – 15 19 38 11 1 4 1 7 15 10 3 8 1 2 2 4
Unique Cell Count 307 638 327 243 248 363 406 382 330 343 305 298 343 412 332 342 315 334 643 347 332 264 336
Labelled Cell Count 310 653 339 258 269 402 444 477 392 423 357 305 354 416 374 400 357 334 643 347 332 264 336


Ambiguous, Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 4.6 5.7 5.6 5.1 5.4 5.3 – 4.5 4.8 5.0 4.9 6.2 6.4 6.0 6.4 8.2 7.9 6.0 6.6 7.0
Std Deviation (1e-4) 0.6 1.0 1.0 1.6 1.2 1.0 1.2 – 0.8 1.0 0.9 1.0 1.0 1.5 1.1 1.4 1.6 1.6 1.2 1.7 2.0
Intensity Change (Log2) – – – -0.0 -0.15 -0.06 -0.09 – -0.34 -0.22 -0.18 -0.21 0.12 0.18 0.09 0.18 0.53 0.49 0.09 0.22 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – 3.5 2.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7888 1.3969 1.1667 0.7294 1.0859 1.1741 5.7061 5.7747 5.9242 6.0787 6.1814 6.0104 2.6237 2.9237 2.7925 2.88 2.8996 3.044
Actin 0.0003 0.0021 0.0005 0.002 0.0012 0.0002 0.0377 0.0042 0.0165 0.0131 0.0142 0.0162 0.0308 0.0009 0.0234 0.0264 0.0216 0.0039
Bud 0.0011 0.0048 0.0005 0.0036 0.0002 0.0001 0.0032 0.006 0.0025 0.0027 0.0061 0.002 0.0037 0.0172 0.0012 0.0009 0.0013 0.0001
Bud Neck 0.0001 0.0003 0.0003 0.0006 0.0002 0.0004 0.0033 0.0005 0.0015 0.0045 0.0005 0.0036 0.0056 0.0002 0.0008 0.0015 0.0104 0.0005
Bud Periphery 0.0005 0.0009 0.0005 0.003 0.0003 0.0001 0.0066 0.0049 0.0038 0.0043 0.0089 0.0041 0.0045 0.005 0.0016 0.0015 0.0031 0.0003
Bud Site 0.0005 0.0119 0.0021 0.0031 0.0001 0.0001 0.0048 0.0292 0.0093 0.0029 0.0026 0.0005 0.0111 0.0152 0.0082 0.0021 0.0065 0.0004
Cell Periphery 0.0003 0.0003 0.0002 0.0002 0.0001 0.0001 0.0006 0.0005 0.0004 0.0002 0.0001 0.0001 0.0008 0.0005 0.0003 0.0002 0.0005 0.0001
Cytoplasm 0.4447 0.5933 0.5622 0.6099 0.6829 0.6202 0.4189 0.5811 0.5147 0.5825 0.5939 0.5969 0.3024 0.5663 0.4817 0.38 0.3616 0.5182
Cytoplasmic Foci 0.0142 0.0119 0.0169 0.0257 0.0139 0.0168 0.023 0.0138 0.0188 0.0188 0.0255 0.0176 0.0259 0.0073 0.0135 0.031 0.0093 0.007
Eisosomes 0.0001 0.0002 0.0002 0 0 0 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0003 0.0001 0.0002 0.0002 0.0003 0.0001
Endoplasmic Reticulum 0.0041 0.0028 0.0046 0.0037 0.0025 0.0041 0.0061 0.0021 0.0041 0.002 0.0023 0.0028 0.0038 0.0045 0.0045 0.0041 0.0035 0.0016
Endosome 0.0075 0.0087 0.0151 0.0258 0.0193 0.013 0.0231 0.0097 0.009 0.0182 0.0112 0.0102 0.0119 0.0024 0.0078 0.0194 0.0158 0.0033
Golgi 0.0004 0.0007 0.0009 0.0015 0.0004 0.0006 0.004 0.0011 0.0013 0.0068 0.0012 0.0036 0.0057 0.0003 0.0056 0.0025 0.0057 0.0029
Lipid Particles 0.0006 0.0003 0.0009 0.0036 0.0002 0.0002 0.0025 0.0004 0.0013 0.0029 0.0034 0.0007 0.0099 0.0004 0.0018 0.0056 0.0075 0.0022
Mitochondria 0.0019 0.0005 0.0011 0.0012 0.0003 0.002 0.0039 0.0006 0.0011 0.0019 0.0014 0.002 0.0027 0.0004 0.0049 0.0012 0.0019 0.0063
None 0.5176 0.3532 0.3851 0.294 0.2745 0.3356 0.4291 0.3338 0.3967 0.3214 0.2743 0.3213 0.5465 0.3705 0.4346 0.5067 0.5187 0.4261
Nuclear Periphery 0.0009 0.0008 0.0019 0.0035 0.0005 0.0016 0.0067 0.0011 0.0025 0.0009 0.0089 0.0031 0.004 0.0016 0.0016 0.0009 0.0084 0.0053
Nucleolus 0.0005 0.0002 0.0002 0.0008 0 0.0001 0.001 0.0005 0.0006 0.0002 0.0012 0.0004 0.0013 0.0004 0.0004 0.0004 0.0016 0.0032
Nucleus 0.0015 0.0024 0.002 0.006 0.0007 0.0013 0.0054 0.0036 0.0037 0.0024 0.0198 0.0026 0.0065 0.0026 0.0015 0.002 0.004 0.0113
Peroxisomes 0.0002 0.0002 0.0002 0.0006 0.0001 0.0001 0.0029 0.0003 0.0046 0.0079 0.002 0.0043 0.0117 0.0001 0.0021 0.0047 0.0033 0.0025
Punctate Nuclear 0.0004 0.0009 0.0009 0.0025 0.0002 0.0005 0.0104 0.0022 0.0035 0.0012 0.0188 0.0059 0.0032 0.0015 0.0022 0.003 0.012 0.0034
Vacuole 0.002 0.0032 0.0033 0.0073 0.0023 0.0024 0.0052 0.0036 0.0034 0.0043 0.003 0.0015 0.0062 0.0023 0.002 0.005 0.0022 0.001
Vacuole Periphery 0.0003 0.0004 0.0006 0.0014 0.0003 0.0006 0.0014 0.0005 0.0006 0.0009 0.0009 0.0006 0.0015 0.0003 0.0004 0.0007 0.0007 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.8107 18.194 17.3581 20.7666 13.1941 21.7943 21.3525 32.3704 36.1088 23.8051
Translational Efficiency 1.3055 0.63 1.1303 1.1902 1.3114 1.4089 1.2647 0.828 1.2579 1.1868

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
639 903 190 1466 1412 2045 2298 728 2051 2948 2488 2194

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 645.14 766.80 993.07 878.67 693.15 820.54 881.89 863.73 678.19 804.08 890.38 873.71
Standard Deviation 76.81 93.09 127.78 129.34 91.05 93.08 108.70 140.25 89.67 96.32 114.16 133.25
Intensity Change Log 2 — 0.249238 0.622283 0.445709 — 0.243406 0.347431 0.317413 — 0.246220 0.486469 0.380685

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000194 0.000368 0.000211 0.001348 0.000115 0.000192 0.000089 0.000308 0.000140 0.000246 0.000098 0.001003
Bud Neck 0.025901 0.029763 0.001826 0.009682 0.005886 0.040594 0.002616 0.003834 0.012122 0.037276 0.002556 0.007741
Bud Site 0.009894 0.030275 0.006565 0.036893 0.005056 0.015931 0.010152 0.023040 0.006563 0.020325 0.009878 0.032297
Cell Periphery 0.000323 0.000152 0.000240 0.000135 0.000136 0.000141 0.000046 0.000092 0.000195 0.000144 0.000061 0.000120
Cytoplasm 0.438698* 0.421046* 0.509342* 0.462956* 0.636741* 0.472876* 0.787873* 0.681759* 0.575040* 0.457000* 0.766602* 0.535558*
Cytoplasmic Foci 0.337957* 0.283258* 0.095433 0.064051 0.179420 0.237291* 0.027713 0.071705 0.228813* 0.251371* 0.032885 0.066591
Eisosomes 0.000417 0.000103 0.000119 0.000061 0.000084 0.000146 0.000013 0.000024 0.000188 0.000133 0.000021 0.000049
Endoplasmic Reticulum 0.000956 0.000789 0.002058 0.001365 0.000960 0.000606 0.000307 0.000524 0.000959 0.000662 0.000441 0.001086
Endosome 0.017007 0.043722 0.015173 0.021706 0.008968 0.026900 0.002147 0.005848 0.011473 0.032053 0.003142 0.016444
Golgi 0.007446 0.009976 0.000269 0.007015 0.001446 0.005773 0.000173 0.004258 0.003315 0.007060 0.000180 0.006100
Lipid Particles 0.013051 0.004614 0.003549 0.002652 0.005186 0.005944 0.000309 0.000805 0.007636 0.005537 0.000556 0.002039
Mitochondria 0.018767 0.011071 0.000200 0.011931 0.001534 0.009538 0.000216 0.000194 0.006903 0.010008 0.000215 0.008037
Mitotic Spindle 0.000126 0.003621 0.001383 0.080075 0.000590 0.004589 0.015244 0.027036 0.000445 0.004292 0.014185 0.062476
None 0.003799 0.005047 0.001204 0.004951 0.005341 0.002866 0.004472 0.002047 0.004861 0.003534 0.004222 0.003987
Nuclear Periphery 0.000321 0.000189 0.000737 0.001413 0.000250 0.000308 0.000240 0.000221 0.000272 0.000271 0.000278 0.001017
Nuclear Periphery Foci 0.000228 0.000485 0.005701 0.001914 0.000862 0.000192 0.000899 0.002743 0.000664 0.000282 0.001266 0.002189
Nucleolus 0.000950 0.001041 0.000625 0.000877 0.000716 0.001154 0.000180 0.000263 0.000789 0.001119 0.000214 0.000673
Nucleus 0.077500 0.036363 0.124435 0.058852 0.105133 0.073775 0.087701 0.050048 0.096524 0.062315 0.090506 0.055931
Peroxisomes 0.007671 0.012913 0.000471 0.010441 0.001501 0.007639 0.000865 0.004300 0.003423 0.009255 0.000835 0.008403
Vacuole 0.036060 0.100158 0.229743* 0.219075* 0.039171 0.090685 0.058350 0.119571 0.038202 0.093587 0.071439 0.186058
Vacuole Periphery 0.002735 0.005045 0.000714 0.002607 0.000902 0.002862 0.000396 0.001378 0.001473 0.003531 0.000421 0.002199

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.20 -1.69 -2.80 -2.03 -2.52 -2.08 0.10 -5.78 -3.59 -6.60 -3.74 0.76 -3.46 -2.77 -3.57
Bud Neck -1.02 9.11 6.31 8.40 -7.40 -16.29 5.01 2.14 17.07 -2.48 -13.54 9.74 4.62 17.60 -6.42
Bud Site -7.65 1.21 -7.73 0.38 -7.88 -7.24 -2.80 -6.50 -2.14 -4.88 -10.34 -1.73 -11.22 -3.10 -9.23
Cell Periphery 5.52 3.00 7.89 3.07 6.10 -0.31 10.84 5.32 4.93 -5.47 3.85 12.68 8.46 4.92 -4.64
Cytoplasm 1.22 -2.30 0.60 -0.73 2.76 18.42 -12.58 3.32 -9.73 12.04 15.13 -18.98 10.07 -3.30 28.12
Cytoplasmic Foci 4.81 22.52 30.75 28.34 3.27 -8.73 30.91 13.28 21.89 -11.13 -3.68 41.63 31.19 39.57 -12.73
Eisosomes 6.77 6.48 7.95 9.02 4.61 -7.10 18.25 13.68 14.64 -8.51 3.44 11.04 9.61 14.96 -8.90
Endoplasmic Reticulum 0.65 -3.83 -0.26 -1.68 4.50 2.71 5.48 3.52 0.38 -2.71 2.47 3.84 0.69 -2.12 -3.93
Endosome -8.58 1.39 1.30 9.86 -0.30 -11.80 8.96 3.94 14.58 -4.84 -14.39 11.25 -0.01 13.65 -9.60
Golgi -1.75 7.59 3.50 5.30 -3.97 -8.45 10.07 -0.64 2.89 -1.77 -6.64 10.04 0.62 5.32 -4.33
Lipid Particles 5.35 6.24 7.15 4.51 2.20 -1.23 10.73 8.29 10.47 -7.25 3.23 12.37 9.85 10.24 -10.23
Mitochondria 2.38 6.42 4.29 2.80 -3.96 -7.87 4.55 4.75 9.60 0.94 -2.42 7.12 2.66 5.39 -3.92
Mitotic Spindle -3.37 -2.24 -15.58 -14.78 -15.27 -3.85 -8.04 -7.69 -6.87 -3.95 -4.89 -8.16 -17.21 -16.12 -11.90
None -0.55 1.81 0.28 0.95 -2.39 2.83 1.75 4.45 1.11 3.89 1.49 1.46 2.11 0.67 1.12
Nuclear Periphery 1.78 -5.22 -4.88 -5.75 -2.36 -0.97 -4.37 -5.13 -2.54 -2.03 0.01 -4.63 -5.69 -5.63 -4.68
Nuclear Periphery Foci -4.23 -8.95 -7.89 -7.13 4.41 3.36 -1.62 -5.63 -6.82 -5.26 2.72 -5.54 -8.51 -10.20 -5.87
Nucleolus -0.10 2.34 -1.80 -1.84 -3.11 -0.75 2.55 1.05 2.96 -2.47 -0.74 3.49 -1.30 -0.96 -4.16
Nucleus 8.90 -3.45 4.32 -6.13 5.38 7.83 0.74 10.75 4.88 11.52 10.90 -1.94 12.29 2.35 15.29
Peroxisomes -3.55 9.89 0.40 3.54 -6.85 -11.16 2.55 -2.40 3.72 -3.21 -9.78 7.86 -3.35 3.58 -7.02
Vacuole -12.73 -13.05 -32.08 -20.70 -0.53 -15.39 -14.92 -17.37 -10.15 -10.25 -19.87 -18.90 -36.09 -24.66 -21.89
Vacuole Periphery -3.45 4.91 3.34 6.57 -1.98 -6.37 3.10 0.54 4.62 -1.03 -6.94 5.40 1.77 7.52 -2.85
Standard name
Human Ortholog
Description Mitochondrial intermembrane space protein of unknown function
Localization
Cell Percentages cytoplasm (71%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Mpm1

Mpm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mpm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available