Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.09 – 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0.06 0.05 0 0.12 – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Endosome 0 0 0 0 0.06 0.08 0 0 0 0 0 0 0 0 0.05 0 0 – 0 0 0 0 0 0
Golgi 0 0.1 0.17 0.13 0.12 0 0 0 0 0 0 0 0.21 0.42 0.21 0.48 0.5 – 0 0.08 0.06 0 0.09 0.06
Mitochondria 0.97 0.89 0.83 0.9 0.8 0.79 0.89 0.85 0.9 0.9 0.93 0.93 0.88 0.78 0.76 0.07 0 – 0.87 0.8 0.81 0.71 0.72 0.74
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0.06 0 0 0 0 0.08 0.06 0.08 0.07 0.07 0.08 0.06 0.05 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0.05 0.09 0.07 0.12 0.07 0.08 0.06 0.07 0 0 0.07 0.06 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 – 0 0 0.05 0.1 0.07 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0 0.12 0.1 0 0.1 0.09 0.07 0.05 0 0.05 0 0.06 0.06 0.08 0.15 0.24 0.21 – 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 5 7 4 1 0 1 8 0 0 1 0 1 1 0 3 1 – 3 7 3 4 6 5
Bud 3 2 2 6 6 1 12 21 12 9 6 13 0 1 0 1 0 – 0 0 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 1 1 4 3 4 0 3 2 0 0 0 1 0 – – – – – – –
Cell Periphery 4 12 9 6 4 2 4 7 3 5 1 1 2 4 7 3 3 – 0 0 0 0 0 1
Cytoplasm 2 1 7 8 20 15 12 15 4 11 9 7 2 9 9 0 4 – 0 0 0 0 0 0
Endoplasmic Reticulum 1 4 3 0 1 2 0 1 1 0 1 4 2 4 4 0 1 – 0 1 0 0 1 2
Endosome 1 1 5 5 20 26 10 12 3 0 0 3 2 3 10 1 0 – 3 7 8 6 8 11
Golgi 0 23 37 29 43 6 1 2 0 0 0 2 25 65 41 14 17 – 8 20 13 5 23 16
Mitochondria 193 209 179 205 288 267 418 409 325 265 293 307 106 121 148 2 1 – 171 197 190 97 178 202
Nucleus 0 1 1 1 1 1 4 9 9 2 7 1 0 0 0 0 0 – 0 0 0 0 0 1
Nuclear Periphery 12 8 9 7 13 28 30 37 26 21 25 20 6 4 4 1 1 – 0 0 1 0 2 2
Nucleolus 9 4 6 2 9 18 44 33 42 20 24 20 8 2 5 2 2 – 0 1 0 3 0 2
Peroxisomes 0 3 4 5 9 7 6 12 7 2 1 1 1 4 9 1 1 – 7 4 10 13 17 8
SpindlePole 0 1 3 2 6 8 10 17 5 2 1 1 2 0 2 1 1 – 0 0 1 0 0 0
Vac/Vac Membrane 4 29 21 10 36 30 33 22 16 14 13 20 7 13 30 7 7 – 0 5 2 3 4 12
Unique Cell Count 199 234 215 229 358 336 470 479 361 294 314 330 120 155 196 29 34 197 248 235 138 247 272
Labelled Cell Count 229 303 293 290 458 412 589 609 457 352 385 402 164 231 269 37 39 197 248 235 138 247 272


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 10.9 8.9 8.6 8.8 7.6 7.2 7.1 6.1 6.4 5.9 6.4 8.9 9.2 9.2 10.7 10.9 – 9.1 9.4 9.7
Std Deviation (1e-4) 1.3 1.8 1.3 1.6 1.6 1.9 1.8 1.7 1.5 1.9 1.6 1.6 1.8 1.3 1.8 2.5 2.4 – 1.8 1.8 1.9
Intensity Change (Log2) – – – -0.06 -0.02 -0.24 -0.31 -0.33 -0.55 -0.48 -0.6 -0.47 -0.0 0.05 0.05 0.27 0.29 – 0.03 0.08 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud 0 0 0 0 2.4 1.8 0 0 2.1 0 0 0 0 0 –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Cell Periphery -0.9 0 0 0 0 0 -1.7 0 0 0 -0.8 -0.3 0 0 –
Cytoplasm 0.1 1.3 0.7 -0.5 -0.1 0 0.3 -0.3 -0.8 0 1.2 0.7 0 0 –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Endosome 0 1.9 2.7 0 0.1 0 0 0 0 0 0 1.5 0 0 –
Golgi -1.3 -1.7 -6.6 -9.0 -8.9 -8.1 -7.4 -7.6 -7.3 0.8 5.3 1.0 3.9 4.3 –
Mitochondria 1.9 -0.8 -1.1 2.1 0.7 2.4 2.3 3.7 3.6 1.3 -1.3 -1.9 -8.8 -9.7 –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Nuclear Periphery -0.6 -0.3 1.9 1.2 1.7 1.5 1.4 1.7 1.0 0.3 -0.8 -1.2 0 0 –
Nucleolus 0 -0.2 1.4 3.1 2.2 3.7 2.0 2.4 1.8 1.7 0 -0.2 0 0 –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1.6 0 0 –
SpindlePole 0 0 0 0 1.6 0 0 0 0 0 0 0 0 0 –
Vacuole -2.2 0.1 -0.3 -1.2 -2.6 -2.5 -2.2 -2.6 -1.6 -1.2 -0.5 1.7 0 0 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.4253 8.6533 8.4551 7.9215 7.0388 8.8321 7.0768 7.376 7.2556 6.6314 6.3361 7.3205 6.9828 7.1377 6.4984 5.882 6.1603 6.4063
Actin 0.0168 0.0103 0.0062 0.0002 0.0188 0.0003 0.0109 0.0023 0.0075 0.0029 0.0213 0.0004 0.0299 0.0046 0.012 0.0003 0.0017 0.0064
Bud 0.0002 0.0001 0.0001 0.0005 0.0003 0 0.0001 0.0007 0.0002 0.0001 0.0006 0 0.0004 0.0006 0.0002 0 0.0001 0.001
Bud Neck 0.0004 0.0001 0 0.0002 0.0003 0 0.0003 0.0001 0.0012 0 0.0002 0 0.0016 0.0002 0.0008 0 0.0001 0.0001
Bud Periphery 0.0005 0.0002 0.0001 0.002 0.0007 0 0.0003 0.001 0.0004 0.0001 0.0013 0 0.0007 0.0008 0.0003 0.0001 0.0002 0.0018
Bud Site 0.0011 0.0006 0.0004 0.0004 0.0017 0 0.0008 0.0061 0.002 0.0001 0.0008 0 0.0042 0.0042 0.0012 0 0.0003 0.0007
Cell Periphery 0.0002 0.0001 0.0002 0.0013 0.0002 0 0.0001 0.0002 0.0003 0.0001 0.0001 0 0.0004 0.0002 0.0001 0 0 0.0002
Cytoplasm 0.0009 0 0.0001 0 0.0004 0 0.0005 0.0001 0.0039 0.0003 0.0003 0 0.0018 0.0001 0.0003 0 0.0001 0.0003
Cytoplasmic Foci 0.0089 0.0037 0.0031 0.0014 0.0052 0.0002 0.0061 0.0027 0.009 0.007 0.01 0.0005 0.0122 0.0034 0.0078 0.0077 0.0052 0.0008
Eisosomes 0.0005 0.0002 0.0002 0.0001 0.0004 0.0001 0.0004 0.0001 0.0002 0.0001 0.0003 0.0001 0.0007 0.0002 0.0003 0.0001 0.0001 0.0002
Endoplasmic Reticulum 0.0009 0 0 0 0.0018 0 0.0003 0 0.002 0.004 0.0004 0 0.0023 0.0003 0.0002 0 0.0001 0.0001
Endosome 0.0121 0.0046 0.0019 0.0012 0.0095 0.0014 0.0091 0.0057 0.0165 0.0146 0.0127 0.003 0.0175 0.0098 0.0045 0.002 0.0128 0.0031
Golgi 0.0126 0.0151 0.0037 0.0009 0.0086 0.0112 0.0115 0.0185 0.0153 0.0261 0.0304 0.0091 0.0232 0.0225 0.0154 0.0034 0.0171 0.0144
Lipid Particles 0.0287 0.0098 0.0094 0.0159 0.0235 0.0052 0.0179 0.0088 0.0203 0.0214 0.0108 0.0031 0.0199 0.0114 0.0067 0.0031 0.0042 0.0049
Mitochondria 0.8667 0.9288 0.9678 0.9361 0.8998 0.9755 0.9163 0.9351 0.8927 0.9022 0.8941 0.9723 0.8509 0.9256 0.9332 0.9738 0.953 0.949
None 0.0032 0 0.0001 0.0001 0.0011 0 0.0004 0 0.003 0.0003 0.0002 0 0.0011 0.0001 0.0013 0 0 0.0002
Nuclear Periphery 0.0019 0.0001 0 0 0.003 0 0.0006 0.0001 0.0012 0.0115 0.0002 0.0001 0.0038 0.0011 0.0003 0 0 0.0003
Nucleolus 0.0009 0.0001 0.0003 0.0045 0.0039 0 0.0007 0.0005 0.003 0.0002 0.0001 0 0.0012 0.0009 0.0014 0.0001 0 0.003
Nucleus 0.0006 0 0.0001 0.0008 0.0015 0 0.0003 0.0001 0.0008 0.001 0.0001 0 0.0008 0.0006 0.0003 0 0.0001 0.0042
Peroxisomes 0.0334 0.0171 0.0037 0.0127 0.0129 0.004 0.0175 0.0132 0.0137 0.0027 0.0127 0.008 0.0156 0.0058 0.0114 0.0089 0.0039 0.0037
Punctate Nuclear 0.0029 0 0.0005 0.0001 0.0028 0 0.0015 0 0.0008 0.0004 0.0002 0 0.0017 0.0002 0.0005 0 0.0001 0.0002
Vacuole 0.0013 0.0009 0.0006 0.016 0.0011 0.0002 0.0016 0.0015 0.0035 0.0014 0.0012 0.0004 0.0032 0.0029 0.0006 0.0002 0.0004 0.002
Vacuole Periphery 0.0053 0.0083 0.0013 0.0057 0.0027 0.0017 0.0028 0.003 0.0025 0.0035 0.0019 0.0029 0.0069 0.0047 0.0012 0.0004 0.0005 0.0034

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 30.4835 19.656 15.9116 20.0687 23.3252 14.9836 23.036 23.1692 19.4143 24.177
Translational Efficiency 0.7686 1.2241 1.0948 0.8734 0.8482 2.0222 1.4335 1.2339 1.6761 1.4888

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
37 667 2849 1760 1664 1760 332 1354 1701 2427 3181 3114

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 932.14 1045.25 1303.49 1317.64 958.91 1122.70 1434.00 1277.78 958.33 1101.41 1317.11 1300.31
Standard Deviation 238.85 180.25 266.95 237.82 163.93 191.10 216.16 207.09 165.97 191.33 265.13 225.84
Intensity Change Log 2 — 0.165229 0.483761 0.499338 — 0.227505 0.580578 0.414172 — 0.197144 0.533666 0.456781

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.037383 0.006811 0.002179 0.004603 0.001340 0.003583 0.004110 0.003502 0.002124 0.004470 0.002381 0.004125
Bud Neck 0.001379 0.002329 0.000439 0.001072 0.000752 0.000466 0.012079 0.002935 0.000766 0.000978 0.001654 0.001882
Bud Site 0.002677 0.002494 0.001024 0.003839 0.001887 0.000579 0.012100 0.013344 0.001904 0.001106 0.002180 0.007972
Cell Periphery 0.000191 0.000179 0.000240 0.000385 0.000137 0.000077 0.000322 0.000402 0.000138 0.000105 0.000249 0.000392
Cytoplasm 0.011902 0.000421 0.000165 0.000506 0.000607 0.000239 0.001229 0.001990 0.000853 0.000289 0.000276 0.001151
Cytoplasmic Foci 0.062321 0.019386 0.004229 0.009749 0.007824 0.010526 0.044637 0.016531 0.009009 0.012961 0.008446 0.012698
Eisosomes 0.000141 0.000259 0.000297 0.000219 0.000060 0.000120 0.000178 0.000118 0.000062 0.000158 0.000285 0.000175
Endoplasmic Reticulum 0.006341 0.000218 0.000094 0.000736 0.000116 0.000123 0.000476 0.001003 0.000251 0.000149 0.000134 0.000852
Endosome 0.029761 0.014006 0.001849 0.007465 0.006849 0.008502 0.039760 0.023207 0.007347 0.010015 0.005805 0.014310
Golgi 0.083770 0.036064 0.009490 0.040125 0.013391 0.034346 0.039184 0.065040 0.014922 0.034818 0.012589 0.050958
Lipid Particles 0.020201 0.006548 0.001776 0.002708 0.003450 0.003084 0.008960 0.004929 0.003815 0.004036 0.002526 0.003674
Mitochondria 0.592384* 0.875373* 0.964277* 0.894980* 0.945815* 0.921374* 0.714467* 0.795005* 0.938127* 0.908732* 0.938204* 0.851510*
Mitotic Spindle 0.003551 0.001342 0.000997 0.003706 0.001455 0.001020 0.036561 0.009340 0.001501 0.001109 0.004709 0.006156
None 0.019276 0.000481 0.001071 0.001285 0.000402 0.000385 0.000834 0.001259 0.000813 0.000411 0.001046 0.001274
Nuclear Periphery 0.000587 0.000069 0.000274 0.000910 0.000118 0.000103 0.003640 0.001431 0.000128 0.000094 0.000625 0.001137
Nuclear Periphery Foci 0.015115 0.000138 0.000115 0.000693 0.000158 0.000120 0.002202 0.001057 0.000483 0.000125 0.000333 0.000851
Nucleolus 0.003909 0.000059 0.000115 0.000115 0.000151 0.000062 0.001053 0.000273 0.000233 0.000061 0.000213 0.000184
Nucleus 0.052932 0.000036 0.000028 0.000058 0.000093 0.000032 0.000398 0.000170 0.001243 0.000033 0.000067 0.000107
Peroxisomes 0.032724 0.028291 0.003481 0.003709 0.010242 0.010295 0.034820 0.006524 0.010731 0.015241 0.006752 0.004933
Vacuole 0.021197 0.003227 0.002701 0.008126 0.002279 0.002293 0.023920 0.023878 0.002690 0.002550 0.004915 0.014975
Vacuole Periphery 0.002256 0.002265 0.005159 0.015012 0.002875 0.002671 0.019071 0.028061 0.002862 0.002559 0.006611 0.020686

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.28 1.48 1.38 1.42 -2.81 -3.51 -2.94 -2.70 0.07 0.52 -2.87 -0.37 -2.46 0.44 -2.69
Bud Neck -0.99 1.54 0.50 1.76 -3.95 0.99 -6.70 -4.55 -5.86 5.30 -0.64 -2.73 -3.51 -3.24 -0.87
Bud Site 0.12 1.20 -0.77 -1.06 -3.26 2.03 -3.83 -5.92 -6.90 -0.43 1.17 -0.40 -5.53 -7.02 -5.81
Cell Periphery 0.26 -0.64 -2.12 -2.45 -1.43 1.98 -3.69 -4.87 -6.91 -1.31 1.09 -1.77 -4.48 -5.72 -1.93
Cytoplasm 2.03 2.07 2.02 -0.80 -3.03 1.41 -1.52 -2.17 -2.94 -1.19 2.04 2.06 -0.75 -3.24 -3.25
Cytoplasmic Foci 2.19 2.99 2.70 3.83 -5.45 -2.31 -8.71 -5.59 -3.89 6.42 -3.09 0.49 -3.09 0.16 -4.30
Eisosomes -1.75 -1.86 -1.54 0.75 1.07 -7.04 -6.46 -4.38 0.11 2.77 -6.29 -3.52 -8.29 -0.86 1.70
Endoplasmic Reticulum 1.31 1.34 1.20 -2.01 -2.54 -0.23 -6.12 -4.94 -4.91 -2.84 0.96 1.11 -3.10 -4.30 -4.41
Endosome 1.74 3.14 2.50 3.60 -8.00 -1.32 -7.78 -8.10 -7.25 3.63 -2.25 1.50 -5.63 -3.64 -8.24
Golgi 1.78 2.81 1.63 -0.89 -9.99 -7.34 -5.82 -11.74 -6.30 -4.36 -7.86 1.31 -12.54 -5.03 -14.51
Lipid Particles 1.51 2.07 1.97 2.16 -1.97 0.38 -2.44 -1.24 -1.77 1.75 -0.22 1.47 0.13 0.45 -2.09
Mitochondria -3.85 -5.07 -4.13 -1.74 12.09 4.01 12.75 16.12 13.18 -4.06 4.75 -0.03 13.29 8.96 15.22
Mitotic Spindle 1.53 1.79 -0.15 -3.50 -4.19 0.66 -6.27 -5.51 -5.91 4.73 0.67 -3.87 -5.58 -6.75 -1.59
None 1.00 0.97 0.96 -4.96 -1.36 0.53 -5.94 -2.57 -2.63 -1.25 0.98 -0.49 -0.96 -5.10 -1.32
Nuclear Periphery 1.40 0.84 -0.71 -3.92 -2.73 0.48 -3.74 -4.61 -4.67 2.24 1.20 -3.77 -5.76 -6.00 -2.37
Nuclear Periphery Foci 1.24 1.25 1.20 -2.27 -2.37 0.80 -4.15 -3.63 -3.81 2.04 1.34 0.58 -1.11 -4.16 -2.87
Nucleolus 1.85 1.83 1.83 -3.17 0.02 1.77 -3.68 -1.74 -3.64 3.14 2.58 0.32 0.72 -4.17 0.64
Nucleus 1.79 1.79 1.79 -2.18 -2.67 1.96 -2.74 -1.54 -3.08 1.92 1.83 1.78 1.72 -3.41 -1.79
Peroxisomes 0.31 2.29 2.28 5.79 -0.27 -0.02 -4.17 2.12 2.23 4.88 -2.11 2.37 3.68 6.29 1.89
Vacuole 1.58 1.63 1.21 -3.70 -5.20 -0.02 -6.62 -10.87 -10.97 -0.07 0.32 -3.00 -10.68 -11.55 -8.98
Vacuole Periphery 0.01 -3.03 -8.97 -10.09 -7.30 0.42 -6.13 -13.20 -13.44 -2.81 0.68 -5.25 -15.72 -16.58 -11.47
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit
Localization
Cell Percentages mitochondrion (98%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Mrpl35

Mrpl35


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrpl35-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available