Standard name | |
---|---|
Human Ortholog | |
Description | Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | 8 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 2 | 2 | 2 | 1 | 0 | 2 | 4 | 2 | 1 | 1 | 2 | 1 | 4 | 9 |
Bud | 6 | 1 | 3 | 5 | 5 | 8 | 10 | 9 | 12 | 8 | 8 | 4 | 0 | 1 | 2 | 5 | 6 | 4 | 0 | 1 | 0 | 0 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 5 |
Bud Site | 2 | 0 | 0 | 0 | 0 | 0 | 4 | 3 | 5 | 4 | 4 | 3 | 0 | 0 | 0 | 4 | 2 | 1 | – | – | – | – | – | – |
Cell Periphery | 3 | 2 | 2 | 7 | 4 | 6 | 6 | 2 | 5 | 2 | 3 | 3 | 4 | 4 | 6 | 9 | 12 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 2 | 4 | 4 | 7 | 9 | 8 | 11 | 4 | 9 | 8 | 6 | 4 | 5 | 3 | 2 | 2 | 2 | 0 | 0 | 1 | 0 | 0 | 0 |
Endoplasmic Reticulum | 3 | 2 | 1 | 0 | 0 | 3 | 0 | 2 | 2 | 2 | 0 | 1 | 4 | 5 | 11 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Endosome | 3 | 0 | 5 | 4 | 48 | 19 | 14 | 10 | 5 | 3 | 0 | 6 | 9 | 17 | 30 | 3 | 6 | 5 | 13 | 11 | 3 | 7 | 14 | 24 |
Golgi | 0 | 1 | 10 | 20 | 13 | 3 | 0 | 2 | 1 | 2 | 0 | 2 | 39 | 58 | 56 | 24 | 35 | 11 | 3 | 8 | 2 | 6 | 16 | 15 |
Mitochondria | 282 | 122 | 71 | 169 | 246 | 302 | 405 | 312 | 400 | 456 | 329 | 335 | 172 | 156 | 165 | 3 | 8 | 8 | 242 | 104 | 91 | 114 | 212 | 250 |
Nucleus | 2 | 4 | 1 | 0 | 1 | 7 | 7 | 13 | 6 | 10 | 0 | 2 | 1 | 0 | 2 | 1 | 1 | 1 | 0 | 2 | 0 | 0 | 2 | 1 |
Nuclear Periphery | 23 | 1 | 0 | 4 | 9 | 22 | 35 | 32 | 44 | 27 | 24 | 31 | 16 | 11 | 14 | 0 | 1 | 1 | 7 | 5 | 0 | 1 | 3 | 7 |
Nucleolus | 16 | 2 | 2 | 2 | 5 | 10 | 26 | 27 | 25 | 30 | 30 | 20 | 4 | 6 | 6 | 1 | 5 | 5 | 1 | 0 | 0 | 0 | 5 | 3 |
Peroxisomes | 4 | 0 | 2 | 6 | 20 | 6 | 3 | 4 | 4 | 5 | 0 | 2 | 2 | 6 | 17 | 7 | 11 | 30 | 12 | 2 | 1 | 10 | 30 | 29 |
SpindlePole | 4 | 0 | 5 | 3 | 16 | 11 | 10 | 11 | 7 | 15 | 3 | 8 | 5 | 3 | 6 | 3 | 8 | 7 | 2 | 1 | 0 | 0 | 2 | 6 |
Vac/Vac Membrane | 26 | 22 | 12 | 8 | 46 | 40 | 36 | 21 | 16 | 40 | 18 | 26 | 34 | 57 | 41 | 8 | 8 | 17 | 2 | 7 | 4 | 3 | 10 | 22 |
Unique Cell Count | 295 | 134 | 97 | 185 | 309 | 357 | 452 | 379 | 422 | 504 | 348 | 372 | 206 | 238 | 248 | 59 | 91 | 83 | 292 | 151 | 111 | 150 | 308 | 384 |
Labelled Cell Count | 377 | 161 | 126 | 232 | 420 | 446 | 564 | 462 | 537 | 613 | 429 | 451 | 296 | 330 | 359 | 73 | 111 | 100 | 292 | 151 | 111 | 150 | 308 | 384 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.1 | 7.0 | 6.7 | 6.9 | 6.7 | 5.8 | 5.4 | 6.2 | 5.4 | 5.8 | 5.2 | 5.9 | 7.7 | 7.7 | 7.3 | 8.0 | 9.2 | 8.7 | 6.8 | 7.6 | 7.6 |
Std Deviation (1e-4) | 0.7 | 1.2 | 2.2 | 1.8 | 1.4 | 1.3 | 1.2 | 1.3 | 1.1 | 1.2 | 1.2 | 1.3 | 1.2 | 1.2 | 1.1 | 1.5 | 1.8 | 1.9 | 1.4 | 1.7 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.03 | -0.01 | -0.21 | -0.32 | -0.13 | -0.31 | -0.23 | -0.38 | -0.18 | 0.19 | 0.19 | 0.12 | 0.24 | 0.45 | 0.36 | 0.01 | 0.18 | 0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.1 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 2.6 | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.7 | 1.9 | 0 | 0.4 | 0 |
Golgi | 0.1 | -2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.9 | 2.9 | 2.6 | 4.5 | 4.5 | 0.6 |
Mitochondria | 4.1 | 1.3 | 2.6 | 4.3 | 2.0 | 6.6 | 4.7 | 6.2 | 4.3 | 2.1 | -1.4 | -1.2 | -8.3 | -8.9 | -8.6 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 2.8 | 3.0 | 3.3 | 0 | 2.7 | 2.9 | 2.8 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1.9 | 1.5 | 1.6 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 1.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.8 | 0 | 2.7 | 6.0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.0 | 0.9 |
Vacuole | -2.5 | 0.6 | -0.3 | -1.4 | -2.4 | -3.4 | -1.4 | -2.5 | -1.7 | 0.9 | 2.4 | 1.0 | 0.2 | -0.8 | 1.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.5292 | 6.8648 | 6.7595 | 6.7648 | 5.7748 | 7.0333 | 6.8033 | 7.1929 | 7.0693 | 6.1383 | 5.705 | 6.6073 | 6.213 | 5.8929 | 5.651 | 5.3945 | 5.7693 | 5.7517 |
Actin | 0.0093 | 0.0098 | 0.0097 | 0.0383 | 0.0015 | 0.0011 | 0.0112 | 0.003 | 0.0059 | 0.0459 | 0.0236 | 0.0007 | 0.0286 | 0.0118 | 0.0107 | 0.0015 | 0.023 | 0.0019 |
Bud | 0.0003 | 0.0005 | 0.0002 | 0.0004 | 0.0001 | 0 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0013 | 0 | 0.0005 | 0.0013 | 0.0001 | 0.0001 | 0.0006 | 0 |
Bud Neck | 0.0043 | 0.0004 | 0.0001 | 0.0026 | 0 | 0.0001 | 0.0008 | 0.0001 | 0.0002 | 0.0011 | 0.0005 | 0 | 0.0008 | 0.0002 | 0.0001 | 0.0004 | 0.0004 | 0.0001 |
Bud Periphery | 0.0005 | 0.001 | 0.0003 | 0.0008 | 0.0002 | 0.0001 | 0.0008 | 0.0004 | 0.0003 | 0.0004 | 0.003 | 0.0001 | 0.0008 | 0.0015 | 0.0002 | 0.0003 | 0.0013 | 0.0001 |
Bud Site | 0.0038 | 0.0013 | 0.0012 | 0.0028 | 0.0001 | 0.0001 | 0.0015 | 0.0004 | 0.0004 | 0.0011 | 0.0053 | 0 | 0.0036 | 0.0014 | 0.0003 | 0.0012 | 0.0028 | 0 |
Cell Periphery | 0.0003 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0 |
Cytoplasm | 0.0033 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0015 | 0.0001 | 0.0009 | 0.0001 | 0.0006 | 0 | 0.001 | 0.004 | 0.0002 | 0.0012 | 0.0001 | 0.0078 |
Cytoplasmic Foci | 0.0144 | 0.0034 | 0.009 | 0.0218 | 0.002 | 0.0033 | 0.0186 | 0.0026 | 0.0021 | 0.0024 | 0.005 | 0.0004 | 0.0097 | 0.0114 | 0.0024 | 0.0016 | 0.0022 | 0.0008 |
Eisosomes | 0.0003 | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0005 | 0.0004 | 0.0001 | 0.0007 | 0.0006 | 0.0004 | 0.0001 | 0.0004 | 0.0001 |
Endoplasmic Reticulum | 0.0018 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0052 | 0.0003 | 0.0007 | 0.0004 | 0.0012 | 0 | 0.0011 | 0.0002 | 0.0001 | 0.0086 | 0.0001 | 0.0008 |
Endosome | 0.0295 | 0.0167 | 0.0058 | 0.0079 | 0.0055 | 0.0083 | 0.0228 | 0.0257 | 0.0108 | 0.0077 | 0.0271 | 0.0044 | 0.0196 | 0.0127 | 0.0037 | 0.0112 | 0.0092 | 0.0047 |
Golgi | 0.0212 | 0.055 | 0.0168 | 0.0123 | 0.0101 | 0.017 | 0.0266 | 0.035 | 0.0196 | 0.0129 | 0.0117 | 0.018 | 0.0239 | 0.049 | 0.0148 | 0.0041 | 0.0155 | 0.0269 |
Lipid Particles | 0.027 | 0.0025 | 0.0134 | 0.0125 | 0.0048 | 0.0037 | 0.0261 | 0.0059 | 0.0074 | 0.0068 | 0.0188 | 0.0012 | 0.0399 | 0.0073 | 0.0068 | 0.0047 | 0.0043 | 0.0084 |
Mitochondria | 0.8482 | 0.8862 | 0.9287 | 0.8892 | 0.9338 | 0.9508 | 0.8414 | 0.9083 | 0.921 | 0.9152 | 0.857 | 0.9715 | 0.8102 | 0.8695 | 0.9469 | 0.9417 | 0.9268 | 0.9424 |
None | 0.0009 | 0 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.005 | 0.0001 | 0.0018 | 0.0001 | 0.0025 | 0 | 0.0009 | 0.0001 | 0.0004 | 0.0003 | 0.0001 | 0 |
Nuclear Periphery | 0.0049 | 0.0001 | 0.0002 | 0.0001 | 0.031 | 0.0011 | 0.0046 | 0.0003 | 0.0024 | 0.0002 | 0.009 | 0 | 0.0022 | 0.0001 | 0.0001 | 0.0077 | 0.0004 | 0.0001 |
Nucleolus | 0.0017 | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0006 | 0.0001 | 0.0004 | 0.0001 | 0.0013 | 0 | 0.007 | 0.0001 | 0.0001 | 0.0004 | 0.0011 | 0 |
Nucleus | 0.0035 | 0.0003 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0.0011 | 0.0001 | 0.0008 | 0.0001 | 0.0015 | 0 | 0.0012 | 0.0001 | 0.0001 | 0.0008 | 0.0009 | 0 |
Peroxisomes | 0.0151 | 0.014 | 0.0117 | 0.0067 | 0.0045 | 0.0059 | 0.0163 | 0.0029 | 0.0056 | 0.0029 | 0.014 | 0.0013 | 0.0239 | 0.0216 | 0.0089 | 0.0072 | 0.0067 | 0.0027 |
Punctate Nuclear | 0.0021 | 0 | 0.0002 | 0.0002 | 0.0031 | 0.0001 | 0.0087 | 0 | 0.0016 | 0.0002 | 0.0052 | 0 | 0.0012 | 0.0003 | 0.001 | 0.0001 | 0.0002 | 0 |
Vacuole | 0.0044 | 0.0047 | 0.0005 | 0.0013 | 0.0004 | 0.0025 | 0.0016 | 0.0063 | 0.0052 | 0.0005 | 0.0061 | 0.0003 | 0.0097 | 0.0015 | 0.0007 | 0.0046 | 0.0015 | 0.0004 |
Vacuole Periphery | 0.0034 | 0.003 | 0.001 | 0.0016 | 0.0014 | 0.0051 | 0.0046 | 0.008 | 0.0122 | 0.0011 | 0.0044 | 0.0017 | 0.013 | 0.0052 | 0.0022 | 0.002 | 0.0023 | 0.0025 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 23.9177 | 19.0053 | 21.8593 | 26.7883 | 33.0777 | 21.2484 | 28.8244 | 29.506 | 28.942 | 30.6876 |
Translational Efficiency | 1.2255 | 1.7131 | 1.39 | 1.2228 | 0.9256 | 1.6775 | 1.2724 | 1.2245 | 1.3762 | 1.3985 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1847 | 311 | 2621 | 1633 | 1593 | 1669 | 962 | 1203 | 3440 | 1980 | 3583 | 2836 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 854.97 | 990.91 | 1159.99 | 1130.48 | 854.48 | 891.33 | 1199.65 | 1183.92 | 854.74 | 906.97 | 1170.64 | 1153.15 |
Standard Deviation | 116.66 | 157.95 | 243.31 | 203.93 | 129.61 | 137.31 | 206.38 | 172.07 | 122.83 | 145.34 | 234.63 | 192.88 |
Intensity Change Log 2 | — | 0.212880 | 0.440167 | 0.402990 | — | 0.060913 | 0.489495 | 0.470453 | — | 0.138918 | 0.465035 | 0.437106 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000720 | 0.004459 | 0.005153 | 0.008940 | 0.001249 | 0.003521 | 0.003378 | 0.008577 | 0.000965 | 0.003668 | 0.004677 | 0.008786 |
Bud Neck | 0.001341 | 0.002250 | 0.001247 | 0.000951 | 0.000620 | 0.002265 | 0.007319 | 0.002379 | 0.001007 | 0.002263 | 0.002877 | 0.001557 |
Bud Site | 0.003976 | 0.006369 | 0.003895 | 0.003447 | 0.001789 | 0.004423 | 0.014737 | 0.012751 | 0.002963 | 0.004729 | 0.006806 | 0.007394 |
Cell Periphery | 0.000254 | 0.000764 | 0.001026 | 0.000815 | 0.000254 | 0.000245 | 0.000549 | 0.000676 | 0.000254 | 0.000327 | 0.000897 | 0.000756 |
Cytoplasm | 0.001003 | 0.001016 | 0.000502 | 0.001088 | 0.000492 | 0.001865 | 0.003545 | 0.002333 | 0.000766 | 0.001732 | 0.001319 | 0.001616 |
Cytoplasmic Foci | 0.012637 | 0.042978 | 0.006031 | 0.005447 | 0.017698 | 0.028366 | 0.023353 | 0.015051 | 0.014981 | 0.030661 | 0.010682 | 0.009521 |
Eisosomes | 0.000035 | 0.000116 | 0.000133 | 0.000184 | 0.000039 | 0.000070 | 0.000136 | 0.000156 | 0.000037 | 0.000077 | 0.000134 | 0.000172 |
Endoplasmic Reticulum | 0.000248 | 0.000383 | 0.000207 | 0.000501 | 0.000223 | 0.000381 | 0.000355 | 0.000444 | 0.000236 | 0.000381 | 0.000247 | 0.000477 |
Endosome | 0.038325 | 0.056143 | 0.007177 | 0.007318 | 0.032571 | 0.050004 | 0.035887 | 0.018488 | 0.035660 | 0.050968 | 0.014885 | 0.012056 |
Golgi | 0.023945 | 0.053845 | 0.015776 | 0.021528 | 0.020592 | 0.045848 | 0.027624 | 0.046784 | 0.022393 | 0.047104 | 0.018957 | 0.032242 |
Lipid Particles | 0.004976 | 0.006204 | 0.001395 | 0.001770 | 0.006400 | 0.006314 | 0.006780 | 0.000677 | 0.005635 | 0.006296 | 0.002841 | 0.001306 |
Mitochondria | 0.873910* | 0.738894* | 0.936977* | 0.922714* | 0.882602* | 0.807825* | 0.808608* | 0.836712* | 0.877935* | 0.796998* | 0.902511* | 0.886233* |
Mitotic Spindle | 0.002796 | 0.011396 | 0.001952 | 0.002659 | 0.002589 | 0.004539 | 0.010819 | 0.010819 | 0.002700 | 0.005616 | 0.004333 | 0.006120 |
None | 0.000165 | 0.000277 | 0.000644 | 0.000557 | 0.000165 | 0.000220 | 0.001237 | 0.000530 | 0.000165 | 0.000229 | 0.000803 | 0.000546 |
Nuclear Periphery | 0.000315 | 0.000167 | 0.000235 | 0.000108 | 0.000170 | 0.000122 | 0.001115 | 0.000479 | 0.000248 | 0.000129 | 0.000471 | 0.000265 |
Nuclear Periphery Foci | 0.000411 | 0.000264 | 0.000161 | 0.000116 | 0.000187 | 0.000390 | 0.000972 | 0.000358 | 0.000307 | 0.000370 | 0.000378 | 0.000219 |
Nucleolus | 0.000122 | 0.000362 | 0.000169 | 0.000057 | 0.000043 | 0.000396 | 0.000702 | 0.000273 | 0.000085 | 0.000390 | 0.000312 | 0.000149 |
Nucleus | 0.000204 | 0.000182 | 0.000139 | 0.000041 | 0.000036 | 0.000561 | 0.001188 | 0.000610 | 0.000126 | 0.000502 | 0.000421 | 0.000283 |
Peroxisomes | 0.017302 | 0.054417 | 0.005661 | 0.011832 | 0.022714 | 0.030733 | 0.019789 | 0.016583 | 0.019808 | 0.034453 | 0.009454 | 0.013848 |
Vacuole | 0.006903 | 0.011575 | 0.005089 | 0.003436 | 0.003587 | 0.006003 | 0.018700 | 0.012605 | 0.005367 | 0.006879 | 0.008744 | 0.007325 |
Vacuole Periphery | 0.010414 | 0.007937 | 0.006429 | 0.006490 | 0.005979 | 0.005909 | 0.013208 | 0.012712 | 0.008360 | 0.006228 | 0.008250 | 0.009129 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.69 | -6.20 | -6.30 | -2.38 | -2.58 | -3.27 | -2.38 | -4.77 | -3.04 | -2.96 | -4.42 | -6.44 | -7.86 | -4.44 | -3.62 |
Bud Neck | -1.73 | 0.27 | 1.26 | 2.84 | 1.52 | -2.56 | -5.54 | -3.45 | -0.28 | 3.65 | -2.23 | -4.92 | -2.09 | 1.11 | 3.03 |
Bud Site | -1.29 | 0.07 | 0.54 | 1.59 | 0.51 | -2.98 | -5.38 | -5.39 | -4.11 | 0.55 | -2.30 | -4.24 | -4.38 | -2.53 | -0.58 |
Cell Periphery | -1.00 | -2.16 | -3.88 | -0.10 | 0.55 | 0.10 | -2.28 | -2.09 | -2.32 | -0.59 | -0.79 | -2.43 | -4.17 | -3.08 | 0.49 |
Cytoplasm | -0.14 | 1.60 | -0.22 | -0.13 | -1.93 | -2.71 | -3.07 | -3.11 | -0.71 | 0.97 | -2.21 | -1.70 | -2.59 | 0.18 | -0.80 |
Cytoplasmic Foci | -5.43 | 5.53 | 6.02 | 6.76 | 0.65 | -4.80 | -2.22 | 1.29 | 5.98 | 3.23 | -8.34 | 3.74 | 4.87 | 11.60 | 1.14 |
Eisosomes | -3.22 | -7.92 | -10.70 | -2.33 | -2.80 | -7.26 | -5.13 | -2.18 | -1.59 | -0.34 | -7.74 | -9.35 | -5.62 | -3.86 | -1.47 |
Endoplasmic Reticulum | -1.34 | 0.92 | -1.78 | -0.74 | -2.10 | -1.25 | -2.03 | -2.78 | -0.53 | -1.23 | -1.40 | -0.31 | -2.71 | -0.79 | -2.68 |
Endosome | -2.74 | 12.78 | 12.41 | 7.98 | -0.13 | -5.02 | -0.89 | 5.24 | 10.44 | 5.21 | -5.23 | 10.94 | 12.96 | 15.26 | 2.29 |
Golgi | -4.63 | 3.94 | 0.94 | 4.92 | -2.41 | -8.57 | -2.56 | -6.79 | -0.20 | -4.48 | -9.46 | 2.16 | -4.45 | 4.88 | -5.94 |
Lipid Particles | -0.62 | 3.60 | 2.91 | 2.30 | -0.73 | 0.05 | -0.28 | 5.37 | 4.99 | 6.23 | -0.55 | 3.59 | 5.57 | 4.83 | 3.74 |
Mitochondria | 6.85 | -9.75 | -6.69 | -9.40 | 2.48 | 7.91 | 6.55 | 4.71 | -2.58 | -2.20 | 10.15 | -4.44 | -1.30 | -10.90 | 2.89 |
Mitotic Spindle | -3.23 | 1.59 | 0.19 | 3.26 | -1.13 | -2.77 | -4.64 | -5.09 | -3.81 | -0.10 | -4.14 | -2.76 | -4.35 | -0.63 | -2.05 |
None | -3.45 | -4.29 | -12.01 | -5.84 | 0.75 | -1.19 | -2.90 | -6.80 | -4.52 | 1.88 | -1.69 | -4.96 | -12.83 | -6.57 | 1.94 |
Nuclear Periphery | 0.87 | 0.45 | 1.23 | 1.91 | 2.24 | 1.49 | -3.08 | -1.54 | -1.79 | 1.73 | 1.29 | -1.74 | -0.14 | -1.57 | 1.64 |
Nuclear Periphery Foci | 0.58 | 1.27 | 1.49 | 2.66 | 1.35 | -1.32 | -3.73 | -1.95 | 0.05 | 2.57 | -0.41 | -0.60 | 0.68 | 1.08 | 2.05 |
Nucleolus | -1.00 | -0.80 | 1.33 | 1.12 | 2.68 | -2.09 | -3.87 | -2.45 | 0.58 | 2.14 | -2.11 | -3.81 | -1.38 | 1.67 | 2.32 |
Nucleus | -0.42 | 0.47 | 1.73 | 1.23 | 1.34 | -2.36 | -2.46 | -1.92 | -0.33 | 0.83 | -2.01 | -2.05 | -1.26 | 0.83 | 0.52 |
Peroxisomes | -4.77 | 5.66 | 2.15 | 5.51 | -3.43 | -2.44 | 0.90 | 1.95 | 4.22 | 1.05 | -5.24 | 6.26 | 3.01 | 7.48 | -2.77 |
Vacuole | -1.50 | 2.10 | 3.91 | 2.64 | 2.20 | -3.17 | -8.51 | -5.65 | -3.96 | 2.57 | -1.81 | -4.64 | -2.37 | -0.48 | 1.44 |
Vacuole Periphery | 1.36 | 3.72 | 3.33 | 0.80 | -0.06 | 0.07 | -5.56 | -5.07 | -5.08 | 0.30 | 2.60 | 0.13 | -0.89 | -3.21 | -1.02 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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